Developmental and epileptic encephalopathy, 12

disease
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Also known as DEE12developmental and epileptic encephalopathy 12early infantile epileptic encephalopathy 12early infantile epileptic encephalopathy caused by mutation in PLCB1EIEE12epileptic encephalopathy, early infantile, 12epileptic encephalopathy, early infantile, type 12PLCB1 early infantile epileptic encephalopathy

Summary

Developmental and epileptic encephalopathy, 12 (MONDO:0013389) is a disease caused by PLCB1 (GenCC Strong), with 7 cohort genes.

At a glance

  • Causal gene: PLCB1 (GenCC Strong)
  • Cohort genes: 7
  • ClinVar variants: 1,948

Clinical features

No curated clinical features (Orphanet) for this disease.

Identifiers

Disease identifiers

FieldValue
Canonical namedevelopmental and epileptic encephalopathy, 12
Mondo IDMONDO:0013389
OMIM613722
DOIDDOID:0080459
UMLSC3150988
MedGen462338
GARD0013318
Is cancer (heuristic)no

Also known as: DEE12 · developmental and epileptic encephalopathy 12 · developmental and epileptic encephalopathy, 12 · early infantile epileptic encephalopathy 12 · early infantile epileptic encephalopathy caused by mutation in PLCB1 · EIEE12 · epileptic encephalopathy, early infantile, 12 · epileptic encephalopathy, early infantile, type 12 · PLCB1 early infantile epileptic encephalopathy

Data availability: 1,948 ClinVar variants · 4 GenCC gene-disease records.

Disease family

Classification path: disease › human disease › disease by body system or component › nervous system disordercentral nervous system disorderbrain disorderepilepsyepilepsy syndrome › neonatal epilepsy syndrome › malignant migrating partial seizures of infancydevelopmental and epileptic encephalopathy, 12

Related subtypes (2): developmental and epileptic encephalopathy, 16, developmental and epileptic encephalopathy, 34

Genetics & variants

GWAS landscape

No GWAS associations recorded — common-variant (GWAS) studies don’t cover this disease (typical for Mendelian / rare diseases). See the curated gene cohort and Mendelian overlap below.

Variant details and genetic-evidence tiers

ClinVar germline variants

600 retrieved; paginated sample, class counts are floors:

256 likely benign, 240 uncertain significance, 30 benign/likely benign, 25 conflicting classifications of pathogenicity, 19 benign, 17 pathogenic, 8 likely pathogenic, 5 pathogenic/likely pathogenic

ClinVarVariant (HGVS)GeneClassificationReview
1070278NC_000020.10:g.(?8608931)(8639361_?)delPLCB1Pathogeniccriteria provided, single submitter
1435065NM_015192.4(PLCB1):c.2666del (p.Lys889fs)PLCB1Pathogeniccriteria provided, single submitter
1451701NM_015192.4(PLCB1):c.967del (p.Ser323fs)PLCB1Pathogeniccriteria provided, single submitter
1456484NC_000020.10:g.(?8639165)(8698515_?)delPLCB1Pathogeniccriteria provided, single submitter
1069274NM_007254.4(PNKP):c.984del (p.Leu329fs)PNKPPathogeniccriteria provided, single submitter
1071624NM_007254.4(PNKP):c.318G>A (p.Trp106Ter)PNKPPathogeniccriteria provided, single submitter
1071794NM_007254.4(PNKP):c.1059C>A (p.Cys353Ter)PNKPPathogeniccriteria provided, single submitter
1074930NC_000019.9:g.(?50364485)(50370481_?)delPNKPPathogeniccriteria provided, single submitter
1075417NM_007254.4(PNKP):c.1299-2A>GPNKPPathogeniccriteria provided, single submitter
1323477NM_007254.4(PNKP):c.1386+1G>CPNKPPathogenic/Likely pathogeniccriteria provided, multiple submitters, no conflicts
1371267NM_007254.4(PNKP):c.1311T>A (p.Cys437Ter)PNKPPathogeniccriteria provided, single submitter
1382516NM_007254.4(PNKP):c.1066G>T (p.Glu356Ter)PNKPPathogenic/Likely pathogeniccriteria provided, multiple submitters, no conflicts
1412103NM_007254.4(PNKP):c.1390C>T (p.Arg464Ter)PNKPPathogeniccriteria provided, single submitter
1419965NM_007254.4(PNKP):c.808C>T (p.Gln270Ter)PNKPPathogeniccriteria provided, single submitter
1452109NM_007254.4(PNKP):c.1448+2T>APNKPPathogeniccriteria provided, single submitter
1452440NM_007254.4(PNKP):c.1543dup (p.Tyr515fs)PNKPPathogeniccriteria provided, single submitter
1453217NM_007254.4(PNKP):c.756_760del (p.Thr253fs)PNKPPathogeniccriteria provided, single submitter
1453676NM_007254.4(PNKP):c.721G>T (p.Glu241Ter)PNKPPathogenic/Likely pathogeniccriteria provided, multiple submitters, no conflicts
1455443NM_007254.4(PNKP):c.1270_1283dup (p.Ala429fs)PNKPPathogeniccriteria provided, single submitter
1455884NM_007254.4(PNKP):c.1395_1396del (p.Glu465fs)PNKPPathogenic/Likely pathogeniccriteria provided, multiple submitters, no conflicts
1460226NM_007254.4(PNKP):c.847del (p.Asp283fs)PNKPPathogeniccriteria provided, single submitter
159788NM_007254.4(PNKP):c.1295_1298+6delPNKPPathogenic/Likely pathogeniccriteria provided, multiple submitters, no conflicts
1481396NM_015192.4(PLCB1):c.465-2A>CPLCB1Likely pathogeniccriteria provided, single submitter
1486443NM_015192.4(PLCB1):c.2208+1G>APLCB1Likely pathogeniccriteria provided, single submitter
1507516NM_015192.4(PLCB1):c.1168-1G>APLCB1Likely pathogeniccriteria provided, single submitter
1068053NM_007254.4(PNKP):c.199-2A>GPNKPLikely pathogeniccriteria provided, single submitter
1467112NM_007254.4(PNKP):c.1298+1G>APNKPLikely pathogeniccriteria provided, single submitter
1497182NM_007254.4(PNKP):c.865+1G>APNKPLikely pathogeniccriteria provided, multiple submitters, no conflicts
1520587NM_007254.4(PNKP):c.1387-2A>GPNKPLikely pathogeniccriteria provided, single submitter
1522783NM_007254.4(PNKP):c.636+1G>CPNKPLikely pathogeniccriteria provided, single submitter

Genes & proteins

Mendelian disease overlap and somatic drivers

GenCC: 6 · Orphanet: 14 · OMIM-shared: 0 · Dual-evidence (GWAS+Mendelian): 0

GenCC gene–disease validity (cohort genes)

the Disease column is the GenCC-asserted condition — a cohort gene’s strongest validity may be for a related predisposition syndrome.

GeneClassificationInheritanceDiseaseRecords
PLCB1StrongAutosomal recessivedevelopmental and epileptic encephalopathy, 126

Orphanet rare-disease linkage (cohort genes)

GeneOrphanet IDRare disease
PLCB1Orphanet:293181Epilepsy of infancy with migrating focal seizures
PLCB1Orphanet:697160Infantile epileptic spasms syndrome
SCN2AOrphanet:140927Self-limited neonatal-infantile epilepsy
SCN2AOrphanet:1934Early infantile developmental and epileptic encephalopathy
SCN2AOrphanet:2131Alternating hemiplegia of childhood
SCN2AOrphanet:293181Epilepsy of infancy with migrating focal seizures
SCN2AOrphanet:306Self-limited infantile epilepsy
SCN2AOrphanet:33069Dravet syndrome
SCN2AOrphanet:36387Genetic epilepsy with febrile seizure plus
SCN2AOrphanet:697160Infantile epileptic spasms syndrome
PLCB4Orphanet:137888Auriculocondylar syndrome
PNKPOrphanet:101101Charcot-Marie-Tooth disease type 2B2
PNKPOrphanet:1934Early infantile developmental and epileptic encephalopathy
PNKPOrphanet:459033Ataxia-oculomotor apraxia type 4

Cohort genes → proteins

7 cohort genes, 6 distinct canonical proteins.

Evidence partition

SubsetGenes
multi_evidence7

Cohort genes (full)

SymbolHGNCEnsemblUniProtNameEvidence
PLCB1HGNC:15917ENSG00000182621Q9NQ661-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1gencc,clinvar
SCN2AHGNC:10588ENSG00000136531Q99250Sodium channel protein type 2 subunit alphaclinvar
ALDH16A1HGNC:28114ENSG00000161618Q8IZ83Aldehyde dehydrogenase family 16 member A1clinvar
AKT1S1HGNC:28426ENSG00000204673Q96B36Proline-rich AKT1 substrate 1clinvar
PTOV1-AS2HGNC:51284ENSG00000269352PTOV1 antisense RNA 2clinvar
PLCB4HGNC:9059ENSG00000101333Q151471-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4clinvar
PNKPHGNC:9154ENSG00000039650Q96T60Bifunctional polynucleotide phosphatase/kinaseclinvar

Cohort function summary

Lead sentence per gene, UniProt-curated.

SymbolProtein nameFunction (lead sentence)
PLCB11-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1Catalyzes the hydrolysis of 1-phosphatidylinositol 4,5-bisphosphate into diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) and mediates intracellular signaling downstream of G protein-coupled receptors.
SCN2ASodium channel protein type 2 subunit alphaMediates the voltage-dependent sodium ion permeability of excitable membranes.
AKT1S1Proline-rich AKT1 substrate 1Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and…
PLCB41-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4Activated phosphatidylinositol-specific phospholipase C enzymes catalyze the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) involved in G-protein coupled receptor signaling pathways.
PNKPBifunctional polynucleotide phosphatase/kinasePlays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways.

Protein-family classification

Druggable: 4 · Difficult: 0 · Unknown: 3 · Druggable fraction: 0.57

Family distribution

Cohort families vs a genome-wide background (hypergeometric, BH-FDR; fold = observed/expected). Counts kept; sorted by enrichment, so the catch-all Other/Unknown bucket no longer leads.

FamilyGenesFoldFDR
Ion channel115.9×0.147
Phosphatase112.0×0.147
Enzyme (other)23.4×0.147
Other/Unknown30.8×0.858

Per-gene assignment

SymbolFamilyDruggable?ECInterPro (top 3)
PLCB1Enzyme (other)yes3.1.4.11C2_dom, PLipase_C_PInositol-sp_X_dom, PI-PLC_fam
SCN2AIon channelyesIQ_motif_EF-hand-BS, Na_channel_asu, Ion_trans_dom
ALDH16A1Other/UnknownnoAldehyde_DH, Aldehyde_DH_dom, Ald_DH/histidinol_DH
AKT1S1Other/UnknownnoAKT1S1, PRAS_NT
PTOV1-AS2Other/Unknownno
PLCB4Enzyme (other)yes3.1.4.11C2_dom, PLipase_C_PInositol-sp_X_dom, PI-PLC_fam
PNKPPhosphataseyes2.7.1.78HAD-SF_hydro_IIIA, PNKP, Polynucleotide_phosphatase

Expression context

Cohort genes with no expression data: 0.

6 cohort genes are a single-cell marker in ≥1 SCXA experiment.

Breadth distribution (Bgee present_calls)

BucketGenes
narrow (1-5 tissues)0
moderate (6-20)0
broad (>20)7
unknown0

Top tissues across cohort

TissueCohort genes
granulocyte3
Brodmann (1909) area 232
parotid gland2
right uterine tube2
endothelial cell1
superior frontal gyrus1
cerebellar vermis1
middle temporal gyrus1
mucosa of transverse colon1
spleen1
gastrocnemius1
hindlimb stylopod muscle1
muscle of leg1
lateral nuclear group of thalamus1
sural nerve1
right adrenal gland cortex1

Per-gene tissue summary (top 30)

SymbolBgee breadthFANTOM5 breadthSCXATop tissues
PLCB1278ubiquitousmarkerendothelial cell, Brodmann (1909) area 23, superior frontal gyrus
SCN2A187broadmarkermiddle temporal gyrus, Brodmann (1909) area 23, cerebellar vermis
ALDH16A1212ubiquitousmarkergranulocyte, spleen, mucosa of transverse colon
AKT1S1222ubiquitousmarkergastrocnemius, hindlimb stylopod muscle, muscle of leg
PTOV1-AS2247yesgranulocyte, parotid gland, right uterine tube
PLCB4273ubiquitousmarkerparotid gland, lateral nuclear group of thalamus, sural nerve
PNKP259ubiquitousmarkerright uterine tube, granulocyte, right adrenal gland cortex

Protein interactions among cohort

Intra-cohort edges: 1.

Hub genes (top 10 by interactor count)

SymbolInteractor count
ALDH16A13,339
SCN2A2,810
PNKP2,445
PLCB12,235
AKT1S11,894
PLCB41,595
PTOV1-AS20

Intra-cohort edges

ABSources
PLCB1PNKPstring_interaction

Structural data

PDB: 3 · AlphaFold-only: 3 · No structure: 1

Cohort genes with PDB structures (top 30)

SymbolUniProtPDB entries
SCN2AQ992505
AKT1S1Q96B364
PNKPQ96T602

AlphaFold-only cohort genes (top 30 by pLDDT)

SymbolUniProtpLDDT
ALDH16A1Q8IZ8391.58
PLCB4Q1514786.03
PLCB1Q9NQ6684.55

Function

Pathway analysis

Distinct Reactome pathways touched by cohort: 34. Enrichment computed across 7 evidence-associated genes (5 with Reactome annotation).

Pathways by enrichment

Over-representation of cohort genes vs the genome-wide background (hypergeometric test, Benjamini-Hochberg FDR; fold = observed/expected over 5 annotated cohort genes). Counts and members are kept as ground-truth; sorted by enrichment.

PathwayCohort genesFoldFDRSample cohort genes
Synthesis of IP3 and IP4 in the cytosol2169.2×0.002PLCB1, PLCB4
PLC beta mediated events2106.2×0.002PLCB1, PLCB4
APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway1326.3×0.024PNKP
Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion1285.5×0.024PLCB1
G alpha (q) signalling events222.9×0.024PLCB1, PLCB4
Acetylcholine regulates insulin secretion1228.4×0.025PLCB1
AKT phosphorylates targets in the cytosol1163.1×0.030AKT1S1
G beta:gamma signalling through PLC beta1114.2×0.031PLCB1
Presynaptic function of Kainate receptors1108.8×0.031PLCB1
mTORC1-mediated signalling195.2×0.031AKT1S1
Constitutive Signaling by AKT1 E17K in Cancer184.6×0.031AKT1S1
Cellular response to heat stress178.8×0.031AKT1S1
PI3K/AKT Signaling in Cancer173.7×0.031AKT1S1
Interaction between L1 and Ankyrins173.7×0.031SCN2A
Phase 0 - rapid depolarisation169.2×0.031SCN2A
Sensory perception of taste167.2×0.031SCN2A
HSF1-dependent transactivation163.4×0.031AKT1S1
Sensory perception of sweet, bitter, and umami (glutamate) taste155.7×0.033SCN2A
MTOR signalling153.1×0.033AKT1S1
Ca2+ pathway135.7×0.047PLCB1
L1CAM interactions124.0×0.066SCN2A
Cardiac conduction121.8×0.070SCN2A
Sensory Perception119.0×0.076SCN2A
Intracellular signaling by second messengers118.3×0.076AKT1S1
Muscle contraction115.4×0.086SCN2A
PIP3 activates AKT signaling113.4×0.095AKT1S1
Diseases of signal transduction by growth factor receptors and second messengers111.4×0.107AKT1S1
Axon guidance19.0×0.129SCN2A
Nervous system development18.6×0.130SCN2A
Cellular responses to stress17.4×0.146AKT1S1

GO biological processes by enrichment

Over-representation of cohort genes vs the genome-wide background (hypergeometric test, Benjamini-Hochberg FDR; fold = observed/expected over 5 annotated cohort genes). Counts and members are kept as ground-truth; sorted by enrichment.

GO termCohort genesFoldFDRSample cohort genes
phosphatidylinositol metabolic process2354.8×7e-04PLCB1, PLCB4
phosphatidylinositol-mediated signaling2280.9×7e-04PLCB1, PLCB4
release of sequestered calcium ion into cytosol2137.6×0.002PLCB1, PLCB4
phospholipase C-activating endothelin receptor signaling pathway13370.4×0.003PLCB4
cellular response to glyceraldehyde13370.4×0.003PLCB1
negative regulation of monocyte extravasation13370.4×0.003PLCB1
memory273.3×0.003PLCB1, SCN2A
intrinsic apoptotic signaling pathway in response to osmotic stress11685.2×0.004SCN2A
phosphatidylinositol catabolic process11685.2×0.004PLCB1
activation of meiosis involved in egg activation11685.2×0.004PLCB1
cellular response to fluoride11685.2×0.004PLCB1
regulation of retrograde trans-synaptic signaling by endocanabinoid11123.5×0.005PLCB1
inositol trisphosphate metabolic process1842.6×0.006PLCB1
positive regulation of developmental growth1842.6×0.006PLCB1
regulation of fertilization1561.7×0.008PLCB1
cellular response to ionomycin1561.7×0.008PLCB1
phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway1421.3×0.008PLCB1
positive regulation of glycoprotein biosynthetic process1421.3×0.008PLCB1
cellular response to vasopressin1421.3×0.008PLCB1
interleukin-12-mediated signaling pathway1374.5×0.008PLCB1
regulation of establishment of endothelial barrier1374.5×0.008PLCB1
ligand-gated ion channel signaling pathway1374.5×0.008PLCB1
interleukin-15-mediated signaling pathway1337.0×0.009PLCB1
negative regulation of cell size1337.0×0.009AKT1S1
positive regulation of acrosome reaction1306.4×0.009PLCB1
response to radiation1240.7×0.011PNKP
base-excision repair, gap-filling1224.7×0.011PNKP
positive regulation of embryonic development1224.7×0.011PLCB1
G protein-coupled acetylcholine receptor signaling pathway1210.7×0.011PLCB1
neurotrophin TRK receptor signaling pathway1210.7×0.011AKT1S1

Therapeutics

Drug target analysis

Approved (phase 4): 1 · Phase ≥3: 1 · Phased (≥1): 1 · Undrugged: 6

Druggability breadth: 5 of 7 evidence-associated genes (71%) have a ChEMBL target (buckets above are over the deeply-mined display cohort).

Genes with an approved drug

The molecule shown is one approved compound that hits the gene — not necessarily a drug of choice or one indicated for this disease.

SymbolExample approved molecule
SCN2ABEPRIDIL

Top cohort targets by molecule count

SymbolMoleculesMax phase
SCN2A994
PLCB100
ALDH16A100
AKT1S100
PTOV1-AS200
PLCB400
PNKP00

Drugs targeting cohort genes (top 30)

MoleculeMax phaseTargets in cohort
BEPRIDIL4SCN2A
DIBUCAINE4SCN2A
ARTICAINE4SCN2A
BUPIVACAINE4SCN2A
IMIPRAMINE4SCN2A
DROPERIDOL4SCN2A
DICYCLOMINE4SCN2A
TETRABENAZINE4SCN2A
PHENIRAMINE4SCN2A
PRILOCAINE4SCN2A
PROPOXYCAINE4SCN2A
PROPARACAINE4SCN2A
HEXYLCAINE4SCN2A
PRAMOXINE4SCN2A
BENOXINATE4SCN2A
QUINIDINE4SCN2A
FELODIPINE4SCN2A
PHENYTOIN4SCN2A
QUININE4SCN2A
NISOLDIPINE4SCN2A
NIFEDIPINE4SCN2A
PRAZOSIN4SCN2A
DILTIAZEM4SCN2A
PRENYLAMINE4SCN2A
COCAINE4SCN2A
TRIFLUOPERAZINE4SCN2A
CINNARIZINE4SCN2A
THIORIDAZINE4SCN2A
ETIDOCAINE4SCN2A
CHLORPHENIRAMINE4SCN2A

Bioactivity and enzyme data

Enzyme cohort genes (≥1 EC): 3.

Cohort genes with ChEMBL bioactivity (full, sorted by assay count)

SymbolAssaysType breakdown
SCN2A203Binding:172, Functional:20, ADMET:10, Toxicity:1
AKT1S114Binding:14
PLCB111Binding:9, Functional:2
PNKP8Binding:8
ALDH16A13Binding:3

Cohort enzymes (BRENDA EC)

SymbolEC numbersNames
PLCB13.1.4.11phosphoinositide phospholipase C
PLCB43.1.4.11phosphoinositide phospholipase C
PNKP2.7.1.78, 3.1.3.32polynucleotide 5’-hydroxyl-kinase, polynucleotide 3’-phosphatase

Cohort genes with high screening signal

≥100 ChEMBL assays — a studied-ness signal; see Therapeutics for approved-drug status.

SymbolChEMBL assays
SCN2A203

Pharmacogenomics

Cohort genes with a PharmGKB record: 6; with CPIC/DPWG dosing guidelines: 0.

No cohort gene has a CPIC/DPWG genotype-guided dosing guideline (PharmGKB).

Chemical tractability of cohort targets

30 approved/phased compounds have measured bioactivity against a cohort gene (and aren’t yet in disease-level trials). This is a research / tractability signal, NOT a therapeutic recommendation — a bioactivity row often reflects off-target or screening binding (e.g. promiscuous kinase inhibitors against a cohort kinase), implying no disease mechanism.

CompoundMax phaseCohort target (bioactivity)
BEPRIDIL4SCN2A
DIBUCAINE4SCN2A
ARTICAINE4SCN2A
BUPIVACAINE4SCN2A
IMIPRAMINE4SCN2A
DROPERIDOL4SCN2A
DICYCLOMINE4SCN2A
TETRABENAZINE4SCN2A
PHENIRAMINE4SCN2A
PRILOCAINE4SCN2A
PROPOXYCAINE4SCN2A
PROPARACAINE4SCN2A
HEXYLCAINE4SCN2A
PRAMOXINE4SCN2A
BENOXINATE4SCN2A
QUINIDINE4SCN2A
FELODIPINE4SCN2A
PHENYTOIN4SCN2A
QUININE4SCN2A
NISOLDIPINE4SCN2A
NIFEDIPINE4SCN2A
PRAZOSIN4SCN2A
DILTIAZEM4SCN2A
PRENYLAMINE4SCN2A
COCAINE4SCN2A
TRIFLUOPERAZINE4SCN2A
CINNARIZINE4SCN2A
THIORIDAZINE4SCN2A
ETIDOCAINE4SCN2A
CHLORPHENIRAMINE4SCN2A

Druggability pyramid

Cohort genes binned by druggability tier (high → low):

TierDefinitionGenesSymbols
AApproved (phase 4 drug)1SCN2A
BPhased (≥1) drug, not yet approved0
CDruggable family + PDB, no drug1PNKP
DDruggable family + AlphaFold only, no drug2PLCB1, PLCB4
EDifficult family or no structure, no drug3ALDH16A1, AKT1S1, PTOV1-AS2

Undrugged target profiles

6 cohort genes are undrugged. Ranked by ‘starting-point quality’ (assay depth + drugged-partner adjacency).

SymbolChEMBL assaysDrugged partners (top 3)
PLCB111
ALDH16A13
AKT1S114
PTOV1-AS20
PLCB40
PNKP8

Clinical trials & evidence

Clinical trials

Clinical trials: 0.