Glasdegib

drug
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Also known as PF-04449913PF-4449913

Summary

Glasdegib (CHEMBL2043437) is an approved small-molecule SMO receptor antagonist (ATC L01XJ03) targeting SMO; indicated across 7 conditions including neoplasm and acute myeloid leukemia.

At a glance

  • Status: Approved (max clinical phase 4)
  • Modality: Small molecule
  • ATC class: L01XJ03
  • Targets: 1 (SMO)
  • Indications: 7 conditions
  • Clinical trials: 25
  • Chemistry: 374.4 Da · C21H22N6O

Identifiers

Drug identity and classification

FieldValue
ChEMBL IDCHEMBL2043437
NameGlasdegib
TypeSmall molecule
Max phase4
FDA approvedyes
PubChem CID25166913
ChEBICHEBI:145428
ATCL01XJ03
Molecular formulaC21H22N6O
Molecular weight374.4
InChIKeySFNSLLSYNZWZQG-VQIMIIECSA-N

SMILES: CN1CC[C@H](C[C@@H]1C2=NC3=CC=CC=C3N2)NC(=O)NC4=CC=C(C=C4)C#N

IUPAC name: 1-[(2R,4R)-2-(1H-benzimidazol-2-yl)-1-methylpiperidin-4-yl]-3-(4-cyanophenyl)urea

ChEBI definition: A member of the class of benzimidazoles that is 1H-benzimidazole which is substituted by a (2R,4S)-4-{[(4-cyanophenyl)carbamoyl]amino}-1-methylpiperidin-2-yl group at position 2. It is a hedgehog signalling pathway inhibitor that acts by binding to Smoothened (SMO) receptors and blocking signal transduction (IC50 = 5 nM). It is used in combination with low-dose cytarabine, for the treatment of newly-diagnosed acute myeloid leukemia (AML) in adult patients (aged ≥ 75 years), or who have medical conditions that prevent the use of standard chemotherapy.

Pharmacological roles (ChEBI): SMO receptor antagonist, Hedgehog signaling pathway inhibitor, antineoplastic agent.

Also known as: Glasdegib, PF-04449913, PF-4449913, GLASDEGIB

Parent form; salt/anhydrous children: CHEMBL2043440, CHEMBL4297534

Patent coverage: 513 distinct patent families (1,290 SureChEMBL compound mentions), from 2 matched compound structure(s). One matched structure accounts for 1,245 (97%) of the total. Mentions count patents naming the compound (not distinct inventions), so promiscuous / reference molecules inflate the mention figure — families are the dedup metric.

Targets

Targets

Primary targets (GtoPdb curated mechanism): the Cancer dependency column is the DepMap CRISPR fitness signal (% of screened cell lines dependent on the target).

GeneTargetActionpAffinityCancer dependencyUniProt
SMOSMOAntagonist8.3Q99835

Broader ChEMBL bioactivity targets: 1 (assay-derived). Sample: Protein smoothened.

Bioactivity

ChEMBL activities: 1 potent at pChembl ≥ 5 of 1 total. Top 30 by potency (10 = 0.1 nM, 6 = 1 µM):

TargetpChemblTypeValueUnitActivity ID
P567268.3IC505nMCHEMBL_ACT_10923808

Target pathways

Aggregated over 1 target gene(s): SMO.

Top Reactome pathways

12 total, by targets touching each:

PathwayTargetsGenes
Signal Transduction1SMO
Organelle biogenesis and maintenance1SMO
Signaling by GPCR1SMO
Class B/2 (Secretin family receptors)1SMO
GPCR ligand binding1SMO
Signaling by Hedgehog1SMO
Hedgehog ‘off’ state1SMO
Cilium Assembly1SMO
Cargo trafficking to the periciliary membrane1SMO
BBSome-mediated cargo-targeting to cilium1SMO
Hedgehog ‘on’ state1SMO
Activation of SMO1SMO

Dominant GO biological processes

GO termTargets
negative regulation of transcription by RNA polymerase II1
vasculogenesis1
osteoblast differentiation1
in utero embryonic development1
cell fate specification1
neural crest cell migration1
negative regulation of protein phosphorylation1
heart looping1
positive regulation of neuroblast proliferation1
positive regulation of mesenchymal cell proliferation1
determination of left/right asymmetry in lateral mesoderm1
type B pancreatic cell development1
protein import into nucleus1
apoptotic process1
smoothened signaling pathway1

Indications & clinical

Indications

7 indications (2 at ChEMBL trial phase 4). Phase below is the highest clinical-trial phase recorded for this drug against each disease — not the molecule’s overall approval status (that is in the Summary).

IndicationTrial phaseMONDOEFO
neoplasm4MONDO:0005070EFO:0000616
acute myeloid leukemia3MONDO:0018874EFO:0000222
soft tissue sarcoma3MONDO:0018078EFO:1001968
myelodysplastic syndrome1MONDO:0018881EFO:0000198
glioblastoma1MONDO:0018177EFO:0000519

2 further indication records had no mapped disease name (EFO/MeSH-only) or were duplicates, and are omitted.

Clinical trials

Total trials: 25.

Phase distribution

PhaseTrials
PHASE19
PHASE27
PHASE34
PHASE1/PHASE24
Not specified1

Top trials by phase / activity

NCTPhaseStatusTitle
NCT03416179PHASE3COMPLETEDA Study Evaluating Intensive Chemotherapy With or Without Glasdegib or Azacitidine With or Without Glasdegib In Patients With Previously Untreated Acute Myeloid Leukemia
NCT03784014PHASE3COMPLETEDMolecular Profiling of Advanced Soft-tissue Sarcomas
NCT04093505PHASE3TERMINATEDGemtuzumab Ozogamicin in Induction and Glasdegib in Postremission Therapy in Patients With AML (Acute Myeloid Leukemia)
NCT04842604PHASE3COMPLETEDContinuation Study of B1371019(NCT03416179) and B1371012(NCT02367456) Evaluating Azacitidine With Or Without Glasdegib In Patients With Previously Untreated AML, MDS or CMML
NCT04231851PHASE2ACTIVE_NOT_RECRUITINGCPX-351 and Glasdegib for Newly Diagnosed Acute Myelogenous Leukemia With MDS Related Changes or Therapy-related Acute Myeloid Leukemia
NCT01546038PHASE2COMPLETEDA Study To Evaluate PF-04449913 With Chemotherapy In Patients With Acute Myeloid Leukemia or Myelodysplastic Syndrome
NCT01841333PHASE2COMPLETEDPF-04449913 For Patients With Acute Myeloid Leukemia at High Risk of Relapse After Donor Stem Cell Transplant
NCT01842646PHASE2COMPLETEDPhase II Hedgehog Inhibitor for Myelodysplastic Syndrome (MDS)
NCT02226172PHASE2TERMINATEDSingle-Agent Glasdegib In Patients With Myelofibrosis Previously Treated With Ruxolitinib
NCT03226418PHASE2COMPLETEDGeriatric Assessment & Genetic Profiling to Personalize Therapy in Older Adults With Acute Myeloid Leukemia
NCT03390296PHASE1/PHASE2COMPLETEDOX40, Venetoclax, Avelumab, Glasdegib, Gemtuzumab Ozogamicin, and Azacitidine in Relapsed or Refractory Acute Myeloid Leukemia
NCT03415867PHASE1/PHASE2UNKNOWNGlasdegib in Refractory Patients With Sclerotic Chronic Graft-Versus-Host Disease
NCT03466450PHASE1/PHASE2COMPLETEDGlasdegib (PF-04449913) With Temozolomide Newly Diagnosed Glioblastoma
NCT04051996PHASE2TERMINATEDGLAD-AML - Glasdegib (Pf-04449913) With Two Standard Decitabine Regimens for Older Patients With Poor-risk Acute Myeloid Leukemia
NCT04111497PHASE1/PHASE2TERMINATEDGlasdegib for Chronic Graft-Versus-Host Disease
NCT00953758PHASE1COMPLETEDA Study Of PF-04449913 In Select Hematologic Malignancies
NCT01286467PHASE1COMPLETEDA Study Of PF-04449913 Administered Alone In Select Solid Tumors
NCT01749085PHASE1COMPLETEDStudy To Determine The Effect Of Food And Strong CYP3A4 Enzyme Inhibitor On PF-04449913 Drug Levels
NCT02038777PHASE1COMPLETEDA Study Of PF-04449913 In Japanese Patients With Select Hematologic Malignancies
NCT02110342PHASE1COMPLETEDA Study To Understand How Radiolabelled PF-04449913 Is Taken Up By The Body, Broken Down And Then Removed From The Body
NCT02367456PHASE1COMPLETEDA Combination Study of PF-04449913 (Glasdegib) and Azacitidine In Untreated MDS, AML and CMML Patients
NCT02430545PHASE1COMPLETEDUnderstanding The Effect Of A Strong CYP3A4 Inducer On Glasdegib Pharmacokinetics
NCT03130556PHASE1COMPLETEDA Study Of Glasdegib In Healthy Volunteers To Establish The Bioequivalence Of The Phase 2 Formulation To The ICH Formulation
NCT03270878PHASE1COMPLETEDGlasdegib Absolute Bioavailability Study
NCT04230564Not specifiedWITHDRAWNAcute Myeloid Leukemia Real World Treatment Patterns

Clinical evidence (CIViC)

No CIViC predictive evidence (expected for non-precision-medicine drugs).

Pharmacology

Pharmacogenomics

No CPIC/DPWG dosing guideline or drug-level clinical/variant annotations in PharmGKB for this molecule.

Molecules sharing ≥1 of this drug’s curated primary targets, merged from two biobtree sources and ranked by shared-target count, then clinical phase: ChEMBL clinical-stage candidates (development phase ≥2) and PubChem drug-class bioactivity (approved / known drugs acting on the target). Deduplicated by drug name; the drug’s own salt forms are excluded. Note: for a drug with few primary targets a shared-target match can reflect off-target / promiscuous binding rather than the same therapeutic mechanism — the phase ordering surfaces bona-fide therapeutics first.

10 molecules share ≥1 primary target. Top 10 by shared-target count:

MoleculeSourceStatusShared targets
INFIGRATINIBChEMBL + PubChemPhase 4 (approved)SMO
SONIDEGIBChEMBL + PubChemPhase 4 (approved)SMO
VISMODEGIBChEMBL + PubChemPhase 4 (approved)SMO
LINIFANIBChEMBLPhase 3SMO
PATIDEGIBChEMBLPhase 3SMO
CEP-32496ChEMBLPhase 2SMO
FORETINIBChEMBLPhase 2SMO
TALADEGIBChEMBLPhase 2SMO
cholecalciferolPubChemApprovedSMO
ErgocalciferolPubChemApprovedSMO