ADAM32

Summary

ADAM32 (ADAM metallopeptidase domain 32, HGNC:15479) is a protein-coding gene on chromosome 8p11.22, encoding Disintegrin and metalloproteinase domain-containing protein 32 (Q8TC27). May play a role in sperm development and fertilization This is a non-catalytic metalloprotease-like protein.

This gene encodes a member of the disintegrin family of membrane-anchored proteins that play a role in diverse biological processes such as brain development, fertilization, tumor development and inflammation. This gene is predominantly expressed in the testis. The encoded protein undergoes proteolytic processing to generate a mature polypeptide comprised of an metalloprotease, disintegrin and epidermal growth factor-like domains. This gene is located in a cluster of other disintegrin and metallopeptidase family genes on chromosome 8. Alternative splicing results in multiple transcript variants encoding different isoforms.

Source: NCBI Gene 203102 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 142 total — 1 pathogenic
• MANE Select transcript: NM_145004

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:15479
Approved symbol	ADAM32
Name	ADAM metallopeptidase domain 32
Location	8p11.22
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000197140
Ensembl biotype	protein_coding
OMIM	618602
Entrez	203102

Gene structure

Transcript identifiers

Ensembl transcripts: 16 — 12 protein_coding, 3 retained_intron, 1 protein_coding_CDS_not_defined

ENST00000379907, ENST00000399831, ENST00000437682, ENST00000517416, ENST00000518259, ENST00000519315, ENST00000520691, ENST00000521741, ENST00000522506, ENST00000523400, ENST00000524303, ENST00000864644, ENST00000864645, ENST00000864646, ENST00000953391, ENST00000953392

RefSeq mRNA: 2 — MANE Select: NM_145004 NM_001313994, NM_145004

CCDS: CCDS47846, CCDS83286

Canonical transcript exons

ENST00000379907 — 25 exons

Exon	Start	End
ENSE00001644801	39233899	39234082
ENSE00001718374	39186909	39187045
ENSE00001740627	39281136	39281174
ENSE00001764341	39232027	39232135
ENSE00001768819	39169916	39169997
ENSE00001800049	39211144	39211324
ENSE00002129166	39107734	39107833
ENSE00002709940	39165030	39165196
ENSE00003462199	39223040	39223238
ENSE00003470638	39270876	39270914
ENSE00003498621	39164764	39164835
ENSE00003499791	39283586	39283624
ENSE00003512359	39118086	39118165
ENSE00003563245	39257187	39257343
ENSE00003566150	39151377	39151548
ENSE00003598611	39254414	39254516
ENSE00003603280	39274312	39274350
ENSE00003605948	39136657	39136718
ENSE00003632102	39275828	39275866
ENSE00003651540	39221610	39221702
ENSE00003671566	39147130	39147205
ENSE00003681295	39149791	39149867
ENSE00003682952	39246083	39246166
ENSE00003683769	39160897	39160965
ENSE00003847739	39284793	39284917

Expression profiles

Bgee: expression breadth ubiquitous, 131 present calls, max score 90.40.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 0.0968 / max 73.9779, expressed in 8 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
88583	0.0884	6
205160	0.0084	4

Top tissues by expression

133 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
left testis	UBERON:0004533	90.40	gold quality
right testis	UBERON:0004534	89.80	gold quality
testis	UBERON:0000473	89.44	gold quality
calcaneal tendon	UBERON:0003701	86.80	gold quality
corpus callosum	UBERON:0002336	80.04	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	78.31	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	74.35	gold quality
endometrium	UBERON:0001295	73.36	gold quality
body of pancreas	UBERON:0001150	71.68	gold quality
superior frontal gyrus	UBERON:0002661	71.39	gold quality
sural nerve	UBERON:0015488	70.88	gold quality
adrenal tissue	UBERON:0018303	70.78	gold quality
colonic epithelium	UBERON:0000397	69.81	gold quality
thoracic mammary gland	UBERON:0005200	69.62	gold quality
primary visual cortex	UBERON:0002436	68.74	gold quality
ascending aorta	UBERON:0001496	68.46	gold quality
thoracic aorta	UBERON:0001515	68.37	gold quality
ventricular zone	UBERON:0003053	67.86	gold quality
subcutaneous adipose tissue	UBERON:0002190	67.64	gold quality
descending thoracic aorta	UBERON:0002345	67.53	gold quality
left adrenal gland cortex	UBERON:0035825	67.53	gold quality
left adrenal gland	UBERON:0001234	67.16	gold quality
adrenal gland	UBERON:0002369	67.16	gold quality
pancreas	UBERON:0001264	66.93	gold quality
rectum	UBERON:0001052	66.73	gold quality
hypothalamus	UBERON:0001898	66.42	gold quality
tibial artery	UBERON:0007610	66.41	gold quality
popliteal artery	UBERON:0002250	66.38	gold quality
right adrenal gland cortex	UBERON:0035827	66.33	gold quality
mucosa of stomach	UBERON:0001199	66.30	gold quality

Single-cell (SCXA)

Detected in 4 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-MTAB-7249	no	281.20
E-GEOD-124858	no	164.56
E-MTAB-7381	no	145.59
E-ANND-3	no	2.04

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

6 targeting ADAM32, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-1252-5P	100.00	69.80	2774
HSA-MIR-5696	99.98	72.36	4487
HSA-MIR-579-3P	99.86	71.66	3628
HSA-MIR-664B-3P	99.84	71.65	3590
HSA-MIR-6125	95.17	67.26	91
HSA-MIR-6808-3P	94.13	65.24	516

Cross-species orthologs

9 orthologs

Organism	Symbol	Gene ID
danio_rerio	adam10b	ENSDARG00000015502
danio_rerio	adam10a	ENSDARG00000053468
danio_rerio	adam9b	ENSDARG00000057138
mus_musculus	Adam32	ENSMUSG00000037437
rattus_norvegicus	Adam32	ENSRNOG00000025728
drosophila_melanogaster	mmd	FBGN0259110
drosophila_melanogaster	kuz	FBGN0259984
caenorhabditis_elegans	sup-17	WBGENE00006324
caenorhabditis_elegans		WBGENE00006804

Paralogs (20): ADAM22 (ENSG00000008277), ADAM28 (ENSG00000042980), ADAM7 (ENSG00000069206), ADAM11 (ENSG00000073670), ADAM2 (ENSG00000104755), ADAM23 (ENSG00000114948), ADAM20 (ENSG00000134007), ADAMDEC1 (ENSG00000134028), ADAM30 (ENSG00000134249), ADAM19 (ENSG00000135074), ADAM10 (ENSG00000137845), ADAM21 (ENSG00000139985), ADAM15 (ENSG00000143537), ADAM12 (ENSG00000148848), ADAM33 (ENSG00000149451), ADAM8 (ENSG00000151651), ADAM17 (ENSG00000151694), ADAM29 (ENSG00000168594), ADAM9 (ENSG00000168615), ADAM18 (ENSG00000168619)

Protein

Protein identifiers

Disintegrin and metalloproteinase domain-containing protein 32 — Q8TC27 (reviewed: Q8TC27)

All UniProt accessions (7): E3W988, E5RGK7, E5RJE6, E5RJY7, E7EPX8, E7ER82, Q8TC27

UniProt curated annotations — full annotation on UniProt →

Function. May play a role in sperm development and fertilization This is a non-catalytic metalloprotease-like protein.

Subcellular location. Membrane.

Tissue specificity. Testis specific.

RefSeq proteins (2): NP_001300923, NP_659441* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000742	EGF	Domain
IPR001590	Peptidase_M12B	Domain
IPR001762	Disintegrin_dom	Domain
IPR002870	Peptidase_M12B_N	Domain
IPR006586	ADAM_Cys-rich	Domain
IPR018358	Disintegrin_CS	Conserved_site
IPR024079	MetalloPept_cat_dom_sf	Homologous_superfamily
IPR034027	Reprolysin_adamalysin	Domain
IPR036436	Disintegrin_dom_sf	Homologous_superfamily

Pfam: PF00200, PF01421, PF01562, PF08516, PF23106

UniProt features (30 total): disulfide bond 7, sequence variant 6, glycosylation site 4, domain 3, compositionally biased region 2, topological domain 2, signal peptide 1, propeptide 1, modified residue 1, chain 1, transmembrane region 1, region of interest 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q8TC27-F1	76.71	0.44

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (1): 17

Disulfide bonds (7): 295–378, 337–362, 339–344, 450–471, 626–636, 630–642, 644–653

Glycosylation sites (4): 39, 125, 465, 598

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 57 (showing top): GOBP_SINGLE_FERTILIZATION, GOMF_METALLOPEPTIDASE_ACTIVITY, GOCC_CELL_SURFACE, GOBP_REPRODUCTIVE_SYSTEM_DEVELOPMENT, GOBP_SPERM_EGG_RECOGNITION, GOBP_SEX_DIFFERENTIATION, GOBP_DEVELOPMENTAL_PROCESS_INVOLVED_IN_REPRODUCTION, GOBP_FERTILIZATION, GOBP_CELL_CELL_RECOGNITION, GOBP_BINDING_OF_SPERM_TO_ZONA_PELLUCIDA, GOBP_MALE_SEX_DIFFERENTIATION, GOBP_DEVELOPMENT_OF_PRIMARY_SEXUAL_CHARACTERISTICS, GOCC_MOTILE_CILIUM, GOCC_SPERM_PRINCIPAL_PIECE, GOBP_PROTEOLYSIS

GO Biological Process (4): proteolysis (GO:0006508), cell adhesion (GO:0007155), binding of sperm to zona pellucida (GO:0007339), male gonad development (GO:0008584)

GO Molecular Function (3): metalloendopeptidase activity (GO:0004222), protein binding (GO:0005515), metallopeptidase activity (GO:0008237)

GO Cellular Component (3): plasma membrane (GO:0005886), cell surface (GO:0009986), membrane (GO:0016020)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	2
protein metabolic process	1
cellular process	1
sperm-egg recognition	1
gonad development	1
development of primary male sexual characteristics	1
endopeptidase activity	1
metallopeptidase activity	1
binding	1
peptidase activity	1
membrane	1
cell periphery	1

Protein interactions and networks

STRING

662 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
ADAM32	IZUMO2	Q6UXV1	560
ADAM32	IZUMO4	Q1ZYL8	535
ADAM32	PLEKHA2	Q9HB19	516
ADAM32	TEKTIP1	A6NCJ1	465
ADAM32	ZPBP2	Q6X784	457
ADAM32	TSACC	Q96A04	448
ADAM32	PLPP5	Q8NEB5	447
ADAM32	TEX55	Q96M34	442
ADAM32	C22orf23	Q9BZE7	434
ADAM32	IZUMO3	Q5VZ72	418
ADAM32	CATSPERT	Q53TS8	399
ADAM32	ZAN	Q9Y493	395
ADAM32	HTRA4	P83105	388
ADAM32	CCDC54	Q8NEL0	387
ADAM32	DDHD2	O94830	382

IntAct

9 interactions, top by confidence:

A	B	Type	Score
CLDN8	ADAM32	psi-mi:“MI:0915”(physical association)	0.560
ADAM32	NUFIP2	psi-mi:“MI:0915”(physical association)	0.400
ADAM32	GPR89A	psi-mi:“MI:0914”(association)	0.350
CDH23	SGTA	psi-mi:“MI:0914”(association)	0.350
TACC3	TNC	psi-mi:“MI:0914”(association)	0.350
CLDN8	ADAM32	psi-mi:“MI:0915”(physical association)	0.000

BioGRID (41): ITGA5 (Affinity Capture-MS), STAT5B (Affinity Capture-MS), ASPHD2 (Affinity Capture-MS), LCLAT1 (Affinity Capture-MS), NOTCH3 (Affinity Capture-MS), JAG2 (Affinity Capture-MS), PLXNA2 (Affinity Capture-MS), KIAA0391 (Affinity Capture-MS), ADAMTS1 (Affinity Capture-MS), ADAM9 (Affinity Capture-MS), INTS4 (Affinity Capture-MS), KTN1 (Affinity Capture-MS), GAS6 (Affinity Capture-MS), IQCB1 (Affinity Capture-MS), BCL2L11 (Affinity Capture-MS)

ESM2 similar proteins: A0A292G9J6, A0A8M9PFP2, A1L2K1, A2A863, A7E2Z9, B0S5G3, B5MFE9, F1R520, F7A4A7, F8VQ03, O93449, O94985, P16144, P35447, P53813, P98089, Q08761, Q0V9V5, Q0VCN6, Q28483, Q3ZCN5, Q5R9Q9, Q5RCW9, Q5RD64, Q61592, Q63772, Q64632, Q6DDW2, Q6DFV8, Q6PZE0, Q6Q0N0, Q8BH34, Q8BJD1, Q8CFM6, Q8CIZ8, Q8CJ69, Q8K410, Q8N2E2, Q8N8U9, Q8R553

Diamond homologs: A2CJE4, C5FUK3, D4B1G0, D4DCV9, F8VQ03, O15072, O43184, O77780, O93517, O93518, P0C6R9, P0DJ87, P0DM89, P17497, P31989, P70505, P97776, Q05910, Q0NZX6, Q0NZX7, Q0NZX8, Q0NZY0, Q13443, Q28478, Q28483, Q28660, Q3TTE0, Q4JCS0, Q4JCS1, Q5BK84, Q60411, Q60472, Q60718, Q60813, Q61072, Q61824, Q63202, Q6NVV9, Q801Z4, Q8JIY1

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

142 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	1
Likely pathogenic	0
Uncertain significance	100
Likely benign	17
Benign	0

Top pathogenic / likely-pathogenic (1)

Variant ID	HGVS	Classification
150494	GRCh38/hg38 8p11.23-11.22(chr8:38046362-39172253)x1	Pathogenic

SpliceAI

4179 predictions. Top by Δscore:

Variant	Effect	Δscore
8:39118162:ATAT:A	donor_gain	1.0000
8:39118166:G:GG	donor_gain	1.0000
8:39147123:T:G	acceptor_gain	1.0000
8:39147126:TCAG:T	acceptor_loss	1.0000
8:39147128:A:T	acceptor_loss	1.0000
8:39147129:GAT:G	acceptor_gain	1.0000
8:39147129:GATA:G	acceptor_gain	1.0000
8:39147129:GATAT:G	acceptor_gain	1.0000
8:39147201:TTCAG:T	donor_loss	1.0000
8:39147202:TCAG:T	donor_loss	1.0000
8:39147203:CAG:C	donor_loss	1.0000
8:39147204:AG:A	donor_loss	1.0000
8:39147205:GG:G	donor_loss	1.0000
8:39147206:GTA:G	donor_loss	1.0000
8:39147207:T:A	donor_loss	1.0000
8:39149861:GAC:G	donor_gain	1.0000
8:39151542:TGAAA:T	donor_gain	1.0000
8:39151549:G:GG	donor_gain	1.0000
8:39160895:A:AG	acceptor_gain	1.0000
8:39160896:G:GG	acceptor_gain	1.0000
8:39164762:A:AG	acceptor_gain	1.0000
8:39164762:AGTAT:A	acceptor_gain	1.0000
8:39164763:G:GG	acceptor_gain	1.0000
8:39164763:GTAT:G	acceptor_gain	1.0000
8:39164763:GTATG:G	acceptor_gain	1.0000
8:39164836:G:GG	donor_gain	1.0000
8:39186907:A:AG	acceptor_gain	1.0000
8:39186908:G:GA	acceptor_gain	1.0000
8:39187041:GTTGT:G	donor_gain	1.0000
8:39187046:G:GG	donor_gain	1.0000

AlphaMissense

5194 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
8:39223217:T:A	C502S	1.000
8:39223218:G:C	C502S	1.000
8:39211304:T:A	C405S	0.999
8:39211305:G:C	C405S	0.999
8:39223085:T:A	C458S	0.999
8:39223086:G:C	C458S	0.999
8:39223124:T:A	C471S	0.999
8:39223125:G:C	C471S	0.999
8:39223181:T:A	C490S	0.999
8:39223182:G:C	C490S	0.999
8:39223217:T:C	C502R	0.999
8:39223218:G:A	C502Y	0.999
8:39223218:G:T	C502F	0.999
8:39223219:T:G	C502W	0.999
8:39232133:G:C	W544C	0.999
8:39232133:G:T	W544C	0.999
8:39233910:G:A	C549Y	0.999
8:39233924:T:A	C554S	0.999
8:39233924:T:C	C554R	0.999
8:39233925:G:A	C554Y	0.999
8:39233925:G:C	C554S	0.999
8:39233926:T:G	C554W	0.999
8:39233999:T:A	C579S	0.999
8:39233999:T:C	C579R	0.999
8:39234000:G:A	C579Y	0.999
8:39234000:G:C	C579S	0.999
8:39234001:C:G	C579W	0.999
8:39234068:T:A	C602S	0.999
8:39234068:T:C	C602R	0.999
8:39234069:G:A	C602Y	0.999

dbSNP variants (sampled 300 via entrez): RS1000004010 (8:39195184 C>T), RS1000035586 (8:39279528 C>T), RS1000050758 (8:39238754 A>G), RS1000053634 (8:39187587 T>C), RS1000062824 (8:39244980 T>C), RS1000073021 (8:39262871 AT>A,ATT), RS1000089788 (8:39180332 G>A,T), RS1000107572 (8:39266594 C>A,T), RS1000125567 (8:39200010 C>A), RS1000157532 (8:39139685 A>G,T), RS1000177539 (8:39189537 C>T), RS1000178780 (8:39143962 G>T), RS1000203106 (8:39232901 A>G), RS1000215476 (8:39148785 T>G), RS1000226672 (8:39276385 G>A,T)

Disease associations

OMIM: gene MIM:618602 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST90006920_3	Herpes simplex virus 2 mgG-1 antibody levels	4.000000e-09

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0009350	Anti-herpes simplex virus 2 IgG measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

GtoPdb / IUPHAR curated pharmacology

(IUPHAR/BPS Guide to Pharmacology — expert-curated)

Target class: enzyme — M12: Astacin/Adamalysin

CTD chemical–gene interactions

19 total (human), top 19 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
aristolochic acid I	increases expression	1
tobacco tar	decreases expression, decreases reaction	1
potassium chromate(VI)	decreases expression	1
diallyl disulfide	decreases expression, decreases reaction	1
nickel sulfate	decreases expression	1
S-(1,2-dichlorovinyl)cysteine	affects cotreatment, increases expression	1
abrine	increases expression	1
jinfukang	affects cotreatment, decreases expression	1
theaflavin-3,3’-digallate	affects expression	1
Benzo(a)pyrene	affects methylation	1
Cisplatin	affects cotreatment, decreases expression	1
Doxorubicin	decreases expression	1
Lipopolysaccharides	affects cotreatment, increases expression	1
Malathion	decreases expression	1
Tobacco Smoke Pollution	increases expression	1
Vanadates	increases expression	1
Aflatoxin B1	increases methylation	1
Okadaic Acid	decreases expression	1
S-Nitrosoglutathione	decreases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.