APMAP

Summary

APMAP (adipocyte plasma membrane associated protein, HGNC:13238) is a protein-coding gene on chromosome 20p11.21, encoding Adipocyte plasma membrane-associated protein (Q9HDC9). Exhibits strong arylesterase activity with beta-naphthyl acetate and phenyl acetate.

Enables arylesterase activity. Located in cell surface and membrane.

Source: NCBI Gene 57136 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 95 total
• Druggable target: yes
• MANE Select transcript: NM_020531

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:13238
Approved symbol	APMAP
Name	adipocyte plasma membrane associated protein
Location	20p11.21
Locus type	gene with protein product
Status	Approved
Aliases	BSCv
Ensembl gene	ENSG00000101474
Ensembl biotype	protein_coding
OMIM	615884
Entrez	57136

Gene structure

Transcript identifiers

Ensembl transcripts: 12 — 12 protein_coding

ENST00000217456, ENST00000451442, ENST00000881535, ENST00000881536, ENST00000881537, ENST00000881538, ENST00000881539, ENST00000881540, ENST00000932672, ENST00000932673, ENST00000932674, ENST00000932675

RefSeq mRNA: 1 — MANE Select: NM_020531 NM_020531

CCDS: CCDS13166

Canonical transcript exons

ENST00000217456 — 9 exons

Exon	Start	End
ENSE00000660965	24970197	24970371
ENSE00000660966	24971460	24971576
ENSE00000660967	24973645	24973737
ENSE00000660968	24978767	24978882
ENSE00000660969	24983903	24984019
ENSE00000859489	24969526	24969660
ENSE00000859490	24992594	24992751
ENSE00001129010	24968892	24969084
ENSE00001635368	24962925	24964022

Expression profiles

Bgee: expression breadth ubiquitous, 295 present calls, max score 99.13.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 57.5974 / max 2260.9711, expressed in 1823 samples.

FANTOM5 promoters (8 alternative TSS)

Promoter ID	TPM avg	Samples expressed
186780	55.2830	1821
186781	1.7370	1168
186776	0.1874	43
186778	0.1177	38
186779	0.0876	28
186774	0.0820	23
186775	0.0554	17
186777	0.0473	19

Top tissues by expression

296 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
cerebellar vermis	UBERON:0004720	99.13	gold quality
right lobe of liver	UBERON:0001114	98.40	gold quality
cerebellar cortex	UBERON:0002129	98.28	gold quality
cerebellar hemisphere	UBERON:0002245	98.27	gold quality
granulocyte	CL:0000094	98.24	gold quality
middle temporal gyrus	UBERON:0002771	98.23	gold quality
cerebellum	UBERON:0002037	98.21	gold quality
right hemisphere of cerebellum	UBERON:0014890	98.06	gold quality
right lobe of thyroid gland	UBERON:0001119	97.99	gold quality
left lobe of thyroid gland	UBERON:0001120	97.83	gold quality
liver	UBERON:0002107	97.79	gold quality
thyroid gland	UBERON:0002046	97.74	gold quality
blood	UBERON:0000178	97.67	gold quality
Brodmann (1909) area 23	UBERON:0013554	97.67	gold quality
bone marrow	UBERON:0002371	97.42	gold quality
upper leg skin	UBERON:0004262	97.23	gold quality
endothelial cell	CL:0000115	97.17	gold quality
bone element	UBERON:0001474	97.02	gold quality
islet of Langerhans	UBERON:0000006	96.80	gold quality
adipose tissue of abdominal region	UBERON:0007808	96.69	gold quality
omental fat pad	UBERON:0010414	96.65	gold quality
peritoneum	UBERON:0002358	96.63	gold quality
adenohypophysis	UBERON:0002196	96.61	gold quality
pituitary gland	UBERON:0000007	96.52	gold quality
adipose tissue	UBERON:0001013	96.39	gold quality
superior frontal gyrus	UBERON:0002661	96.33	gold quality
prefrontal cortex	UBERON:0000451	96.17	gold quality
postcentral gyrus	UBERON:0002581	96.15	gold quality
stromal cell of endometrium	CL:0002255	96.14	gold quality
connective tissue	UBERON:0002384	96.11	gold quality

Single-cell (SCXA)

Detected in 5 experiment(s), a significant marker in 5.

Experiment	Marker?	Max mean expression
E-HCAD-4	yes	94.37
E-HCAD-1	yes	85.31
E-CURD-122	yes	44.34
E-ANND-3	yes	14.93
E-MTAB-9801	yes	9.03

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): PPARG

miRNA regulators (miRDB)

22 targeting APMAP, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-574-5P	100.00	66.01	989
HSA-MIR-190A-3P	100.00	80.35	5520
HSA-MIR-5011-5P	100.00	83.46	5820
HSA-MIR-520G-5P	99.99	66.76	658
HSA-MIR-3119	99.92	71.34	2390
HSA-MIR-8063	99.91	69.76	3146
HSA-MIR-3120-3P	99.54	70.28	2669
HSA-MIR-642A-5P	99.51	65.10	1152
HSA-MIR-4643	99.49	67.63	1791
HSA-MIR-3123	99.47	67.15	2693
HSA-MIR-4326	98.97	67.63	962
HSA-MIR-603	98.58	68.28	1603
HSA-MIR-374C-3P	98.47	67.93	451
HSA-MIR-3138	98.41	67.53	744
HSA-MIR-660-3P	98.14	66.04	1434
HSA-MIR-211-3P	98.14	66.77	1052
HSA-MIR-4518	98.12	66.82	1030
HSA-MIR-943	97.81	64.42	694
HSA-MIR-1266-5P	97.71	66.92	1052
HSA-MIR-764	94.16	64.85	656
HSA-MIR-6767-3P	93.99	66.01	204
HSA-MIR-4281	92.91	63.60	271

Literature-anchored findings (GeneRIF, showing 7)

• characterization of the gene product of open reading frame 3 encoded at human chromosome 20 (C20orf3), which represents a member of the lactonohydrolase super family (PMID:18513186)
• C20orf3 mapping at chromosome 20p11 is associated with hepatic-specific metastasis in patients with colorectal cancer. (PMID:22267596)
• APMAP is an endogenous suppressor of Abeta production in the brain. (PMID:25180020)
• These data suggest that APMAP is a novel regulator of extracellular matrix components, and establish that APMAP is a potential target to mitigate obesity-associated insulin resistance. (PMID:28559441)
• results suggest that APMAP functions as a modulator promoting human cytomegalovirus (HCMV) infection in multiple cell types and is an important player in the complex HCMV infection mechanism (PMID:31356650)
• Adipocyte Plasma Membrane Protein (APMAP) promotes JC Virus (JCPyV) infection in human glial cells. (PMID:32838939)
• Inter-cellular CRISPR screens reveal regulators of cancer cell phagocytosis. (PMID:34497417)

Cross-species orthologs

8 orthologs

Organism	Symbol	Gene ID
danio_rerio	apmap	ENSDARG00000017422
mus_musculus	Apmap	ENSMUSG00000033096
rattus_norvegicus	Apmap	ENSRNOG00000006795
drosophila_melanogaster	Hmu	FBGN0015737
drosophila_melanogaster	Ssl2	FBGN0041780
caenorhabditis_elegans		WBGENE00007438
caenorhabditis_elegans		WBGENE00010197
caenorhabditis_elegans		WBGENE00011739

Protein

Protein identifiers

Adipocyte plasma membrane-associated protein — Q9HDC9 (reviewed: Q9HDC9)

Alternative names: Protein BSCv

All UniProt accessions (2): Q9HDC9, H0Y512

UniProt curated annotations — full annotation on UniProt →

Function. Exhibits strong arylesterase activity with beta-naphthyl acetate and phenyl acetate. May play a role in adipocyte differentiation.

Subcellular location. Membrane.

Tissue specificity. Liver, glomerular and tubular structures of the kidney, endothelial cells, arterial wall and pancreatic islets of Langerhans (at protein level). Found ubiquitously in adult as well as in embryonic tissues. In adult tissue, the highest expression is found in the liver, placenta and heart. Found on the cell surface of monocytes. In embryonic tissue, the highest expression levels is found in the liver and the kidney.

Similarity. Belongs to the strictosidine synthase family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q9HDC9-1	1	yes
Q9HDC9-2	2

RefSeq proteins (1): NP_065392* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR011042	6-blade_b-propeller_TolB-like	Homologous_superfamily
IPR018119	Strictosidine_synth_cons-reg	Domain

Pfam: PF03088, PF20067

UniProt features (19 total): sequence conflict 4, sequence variant 3, splice variant 2, topological domain 2, modified residue 2, glycosylation site 2, initiator methionine 1, chain 1, transmembrane region 1, region of interest 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9HDC9-F1	91.60	0.84

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (2): 2, 19

Glycosylation sites (2): 160, 196

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 132 (showing top): YAO_TEMPORAL_RESPONSE_TO_PROGESTERONE_CLUSTER_10, MODULE_151, GRAESSMANN_APOPTOSIS_BY_SERUM_DEPRIVATION_UP, GOCC_CELL_SURFACE, WEI_MYCN_TARGETS_WITH_E_BOX, DEURIG_T_CELL_PROLYMPHOCYTIC_LEUKEMIA_DN, ROZANOV_MMP14_TARGETS_UP, GATA6_01, DODD_NASOPHARYNGEAL_CARCINOMA_UP, GCM_NF2, GRYDER_PAX3FOXO1_ENHANCERS_IN_TADS, BURTON_ADIPOGENESIS_6, MODULE_114, TGGAAA_NFAT_Q4_01, GOMF_HYDROLASE_ACTIVITY_ACTING_ON_ESTER_BONDS

GO Biological Process (0):

GO Molecular Function (2): arylesterase activity (GO:0004064), protein binding (GO:0005515)

GO Cellular Component (2): cell surface (GO:0009986), membrane (GO:0016020)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	2
carboxylic ester hydrolase activity	1
binding	1

Protein interactions and networks

STRING

2912 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
APMAP	PTGFRN	Q9P2B2	546
APMAP	THNSL1	Q8IYQ7	477
APMAP	LOXL1	Q08397	475
APMAP	APP	P05067	468
APMAP	ZNF841	Q6ZN19	400
APMAP	IGSF3	O75054	394
APMAP	COL5A2	P05997	390
APMAP	SRP54	P13624	390
APMAP	TUFM	P49411	382
APMAP	CS	O75390	373
APMAP	TPI1	P00938	369
APMAP	PECR	Q9BY49	362
APMAP	ZNF548	Q8NEK5	360
APMAP	SPOPL	Q6IQ16	356
APMAP	PTPRC	P08575	353
APMAP	TSHR	P16473	353

IntAct

80 interactions, top by confidence:

A	B	Type	Score
TSPAN15	ADAM10	psi-mi:“MI:0914”(association)	0.840
CFTR	ESYT2	psi-mi:“MI:2364”(proximity)	0.710
CFTR	ESYT2	psi-mi:“MI:0914”(association)	0.710
FAM209A	APMAP	psi-mi:“MI:0915”(physical association)	0.560
sseJ	AGPS	psi-mi:“MI:0914”(association)	0.460
SRPK1	APMAP	psi-mi:“MI:0217”(phosphorylation reaction)	0.440
TCOF1	APMAP	psi-mi:“MI:0915”(physical association)	0.400
	GNAT3	psi-mi:“MI:0915”(physical association)	0.400
	TK2	psi-mi:“MI:0915”(physical association)	0.400
APMAP	SMAD3	psi-mi:“MI:0915”(physical association)	0.370
	ESYT2	psi-mi:“MI:0914”(association)	0.350
E5	ESYT2	psi-mi:“MI:0914”(association)	0.350
	PGRMC1	psi-mi:“MI:0914”(association)	0.350
	HAX1	psi-mi:“MI:0914”(association)	0.350
INTU	PSMD12	psi-mi:“MI:0914”(association)	0.350
ADAM10	TSPAN9	psi-mi:“MI:0914”(association)	0.350
APBB1	SSPOP	psi-mi:“MI:0914”(association)	0.350
CD177	MYO1G	psi-mi:“MI:0914”(association)	0.350
TEX101	GGT3P	psi-mi:“MI:0914”(association)	0.350
APMAP		psi-mi:“MI:0914”(association)	0.350
TAGLN	LOC392647	psi-mi:“MI:0914”(association)	0.350
SLC16A11	ESYT2	psi-mi:“MI:0914”(association)	0.350

BioGRID (101): APMAP (Affinity Capture-RNA), APMAP (Affinity Capture-RNA), APMAP (Affinity Capture-MS), APMAP (Co-fractionation), APMAP (Co-fractionation), CANX (Co-fractionation), CLGN (Co-fractionation), HNRNPDL (Co-fractionation), APMAP (Affinity Capture-MS), APMAP (Proximity Label-MS), INTU (Affinity Capture-MS), APMAP (Affinity Capture-RNA), APMAP (Affinity Capture-MS), APMAP (Affinity Capture-MS), APMAP (Affinity Capture-MS)

ESM2 similar proteins: A0A2R8QP51, A4IG53, B5X3B2, F1N2K1, F4ZGF2, O95497, P27169, P27170, P52430, P54832, P55159, P70665, P82450, P98192, Q08C93, Q15165, Q15166, Q1LWG4, Q2TAA5, Q3T0E5, Q3TZM9, Q4V3D9, Q58CQ9, Q58DS7, Q5I047, Q5R748, Q5R7Z6, Q5RFU0, Q5ZIF1, Q5ZK82, Q62086, Q62087, Q68FP2, Q6AXM8, Q6P9A2, Q7TP48, Q803F5, Q8K1B9, Q90678, Q90952

Diamond homologs: B5X3B2, F4IJZ6, P18417, P68174, P68175, P94111, Q3T0E5, Q4V3D9, Q5ZIF1, Q7TP48, Q803F5, Q8VWF6, Q9D7N9, Q9HDC9, Q9M1B4, Q9M1J6, Q9M1J7, Q9SD05, Q9SLG8, Q9VB46, P92976, Q9CAZ7, Q9SD04, Q9SD07

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 103 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Signaling by Interleukins	8	7.5×	6e-03

GO biological processes:

GO term	Partners	Fold	FDR
intra-Golgi vesicle-mediated transport	5	29.6×	3e-04
positive regulation of miRNA transcription	6	19.6×	3e-04
retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum	5	18.9×	2e-03
hematopoietic progenitor cell differentiation	5	13.3×	8e-03
response to xenobiotic stimulus	8	6.2×	8e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

95 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	79
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1607 predictions. Top by Δscore:

Variant	Effect	Δscore
20:24969522:ACACC:A	donor_loss	1.0000
20:24969523:CACCT:C	donor_loss	1.0000
20:24969524:A:T	donor_loss	1.0000
20:24969525:C:CG	donor_loss	1.0000
20:24969532:AT:A	donor_gain	1.0000
20:24969657:CAGG:C	acceptor_gain	1.0000
20:24969661:C:CC	acceptor_gain	1.0000
20:24970279:T:TA	donor_gain	1.0000
20:24970287:T:TA	donor_gain	1.0000
20:24970379:T:C	acceptor_gain	1.0000
20:24970379:T:TC	acceptor_gain	1.0000
20:24971454:ACAT:A	donor_loss	1.0000
20:24971456:ATACG:A	donor_loss	1.0000
20:24971457:TACGT:T	donor_loss	1.0000
20:24971458:A:AC	donor_gain	1.0000
20:24971458:A:T	donor_loss	1.0000
20:24971459:C:CA	donor_loss	1.0000
20:24971459:C:CC	donor_gain	1.0000
20:24971459:CGTTT:C	donor_gain	1.0000
20:24971474:A:AC	donor_gain	1.0000
20:24971475:C:CC	donor_gain	1.0000
20:24971477:T:TA	donor_gain	1.0000
20:24973643:A:AC	donor_gain	1.0000
20:24973644:C:CC	donor_gain	1.0000
20:24969656:GCAGG:G	acceptor_gain	0.9900
20:24969657:CAGGC:C	acceptor_gain	0.9900
20:24969658:AGGC:A	acceptor_loss	0.9900
20:24969659:GG:G	acceptor_gain	0.9900
20:24969660:GC:G	acceptor_loss	0.9900
20:24969661:C:CA	acceptor_loss	0.9900

AlphaMissense

2716 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
20:24970197:C:G	R238P	0.997
20:24970256:G:C	S218R	0.997
20:24970256:G:T	S218R	0.997
20:24970258:T:G	S218R	0.997
20:24963897:G:C	S389R	0.995
20:24963897:G:T	S389R	0.995
20:24963899:T:G	S389R	0.995
20:24969025:C:T	G303E	0.994
20:24970201:C:A	G237W	0.994
20:24970260:G:A	S217F	0.994
20:24968963:G:T	R324S	0.993
20:24970200:C:T	G237E	0.992
20:24970260:G:T	S217Y	0.992
20:24969562:A:T	V271D	0.991
20:24969532:A:T	I281K	0.990
20:24969600:G:C	F258L	0.990
20:24969600:G:T	F258L	0.990
20:24969602:A:G	F258L	0.990
20:24970302:A:G	L203P	0.990
20:24971546:C:G	R151T	0.989
20:24963898:C:A	S389I	0.988
20:24970273:A:C	Y213D	0.988
20:24970307:A:C	N201K	0.988
20:24970307:A:T	N201K	0.988
20:24963936:G:C	S376R	0.987
20:24963936:G:T	S376R	0.987
20:24963938:T:G	S376R	0.987
20:24968962:C:G	R324P	0.987
20:24969007:C:G	R309P	0.987
20:24969592:C:T	G261E	0.987

dbSNP variants (sampled 300 via entrez): RS1000053456 (20:24970756 C>T), RS1000158229 (20:24970914 C>T), RS1000232392 (20:24966442 G>C), RS1000263669 (20:24966670 C>G,T), RS1000272313 (20:24982763 G>C), RS1000281051 (20:24994594 G>A), RS1000324029 (20:24982521 C>A,T), RS1000354652 (20:24965000 C>G), RS1000517152 (20:24984080 T>G), RS1000523543 (20:24988671 CT>C), RS1000645733 (20:24978529 G>A), RS1000763617 (20:24972360 T>C), RS1000894856 (20:24988972 C>G,T), RS1001002847 (20:24990463 A>G), RS1001060345 (20:24972475 G>A)

Disease associations

OMIM: gene MIM:615884 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST006585_990	Blood protein levels	2.000000e-19

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067357 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
7.15	Kd	70.13	nM	CHEMBL5653589
7.15	ED50	70.13	nM	CHEMBL5653589

PubChem BioAssay actives

1 with measured affinity, of 2 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2147875: Binding affinity to human APMAP incubated for 45 mins by Kinobead based pull down assay	kd	0.0701	uM

CTD chemical–gene interactions

44 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	affects expression, decreases expression, increases expression	5
bisphenol F	increases expression, affects cotreatment, decreases expression	2
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	increases expression, affects cotreatment	2
Acetaminophen	decreases expression, increases expression	2
Valproic Acid	affects expression, increases expression	2
Cadmium Chloride	decreases expression, increases abundance, increases expression	2
2,4,6-tribromophenol	decreases expression	1
methyleugenol	decreases expression	1
triphenyl phosphate	affects expression	1
pyrogallol 1,3-dimethyl ether	affects cotreatment, affects localization, increases expression	1
sodium arsenite	decreases expression	1
tetrabromobisphenol A	decreases expression	1
benzo(e)pyrene	increases methylation	1
nickel sulfate	decreases expression	1
2,3,5-(triglutathion-S-yl)hydroquinone	increases ADP-ribosylation	1
nutlin 3	affects cotreatment, increases secretion	1
ICG 001	increases expression	1
bisphenol B	increases expression	1
abrine	decreases expression	1
2,2’,4,4’-tetrabromodiphenyl ether	decreases expression	1
pentabrominated diphenyl ether 100	decreases expression	1
hexabrominated diphenyl ether 153	decreases expression	1
bisphenol S	increases expression	1
LDN 193189	affects cotreatment, increases expression	1
bisphenol AF	increases expression	1
Temozolomide	increases expression	1
Benzo(a)pyrene	increases methylation	1
Cadmium	increases expression, increases abundance	1
Dactinomycin	affects cotreatment, increases secretion	1
Dexamethasone	affects cotreatment, decreases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5650917	Binding	Binding affinity to human APMAP incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Cellosaurus cell lines

3 cell lines: 3 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_E1QK	HAP1 APMAP (-) 1	Cancer cell line	Male
CVCL_E1QL	HAP1 APMAP (-) 2	Cancer cell line	Male
CVCL_E1QM	HAP1 APMAP (-) 3	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.