ART5

Summary

ART5 (ADP-ribosyltransferase 5, HGNC:24049) is a protein-coding gene on chromosome 11p15.4, encoding Ecto-ADP-ribosyltransferase 5 (Q96L15).

The protein encoded by this gene belongs to the ARG-specific ADP-ribosyltransferase family. Proteins in this family regulate the function of target proteins by attaching ADP-ribose to specific amino acid residues in their target proteins. The mouse homolog lacks a glycosylphosphatidylinositol-anchor signal sequence and is predicted to be a secretory enzyme. Several transcripts encoding different isoforms have been found for this gene.

Source: NCBI Gene 116969 — RefSeq curated summary.

At a glance

• GWAS associations: 3
• Clinical variants (ClinVar): 66 total
• MANE Select transcript: NM_053017

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:24049
Approved symbol	ART5
Name	ADP-ribosyltransferase 5
Location	11p15.4
Locus type	gene with protein product
Status	Approved
Aliases	ARTC5
Ensembl gene	ENSG00000167311
Ensembl biotype	protein_coding
OMIM	610625
Entrez	116969

Gene structure

Transcript identifiers

Ensembl transcripts: 12 — 12 protein_coding

ENST00000359918, ENST00000397067, ENST00000397068, ENST00000425767, ENST00000453353, ENST00000904765, ENST00000931796, ENST00000931797, ENST00000931798, ENST00000931799, ENST00000931800, ENST00000931801

RefSeq mRNA: 3 — MANE Select: NM_053017 NM_001079536, NM_001297668, NM_053017

CCDS: CCDS73242, CCDS7743

Canonical transcript exons

ENST00000397068 — 4 exons

Exon	Start	End
ENSE00001112017	3639642	3640371
ENSE00001112022	3639003	3639035
ENSE00001402403	3638512	3638793
ENSE00001953691	3641806	3641992

Expression profiles

Bgee: expression breadth ubiquitous, 144 present calls, max score 87.16.

FANTOM5 (CAGE): breadth broad, TPM avg 0.9051 / max 58.4681, expressed in 257 samples.

FANTOM5 promoters (6 alternative TSS)

Promoter ID	TPM avg	Samples expressed
118294	0.3600	93
118295	0.2546	140
118299	0.1816	83
118298	0.0796	30
118297	0.0238	7
118296	0.0055	3

Top tissues by expression

245 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
left testis	UBERON:0004533	87.16	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	86.82	gold quality
right testis	UBERON:0004534	86.25	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	85.09	gold quality
testis	UBERON:0000473	83.95	gold quality
hindlimb stylopod muscle	UBERON:0004252	80.47	gold quality
gastrocnemius	UBERON:0001388	80.03	gold quality
muscle of leg	UBERON:0001383	79.48	gold quality
left ovary	UBERON:0002119	75.51	gold quality
right ovary	UBERON:0002118	73.99	gold quality
apex of heart	UBERON:0002098	73.97	gold quality
ectocervix	UBERON:0012249	71.26	gold quality
endocervix	UBERON:0000458	70.46	gold quality
right lobe of thyroid gland	UBERON:0001119	69.81	gold quality
ovary	UBERON:0000992	69.50	gold quality
body of uterus	UBERON:0009853	68.97	gold quality
oocyte	CL:0000023	67.81	silver quality
left lobe of thyroid gland	UBERON:0001120	67.63	gold quality
thyroid gland	UBERON:0002046	66.37	gold quality
sperm	CL:0000019	66.23	gold quality
stromal cell of endometrium	CL:0002255	64.47	gold quality
uterine cervix	UBERON:0000002	63.93	gold quality
skeletal muscle tissue	UBERON:0001134	63.79	gold quality
muscle tissue	UBERON:0002385	63.45	gold quality
heart left ventricle	UBERON:0002084	63.14	gold quality
trabecular bone tissue	UBERON:0002483	62.75	gold quality
cardiac ventricle	UBERON:0002082	62.36	gold quality
gall bladder	UBERON:0002110	61.86	gold quality
vagina	UBERON:0000996	61.59	gold quality
myocardium	UBERON:0002349	61.14	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	1.12

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

5 targeting ART5, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-95-5P	99.89	72.17	3973
HSA-MIR-6763-5P	99.76	64.68	1767
HSA-MIR-3150A-3P	99.76	64.44	1640
HSA-MIR-6762-3P	99.66	66.94	1188
HSA-MIR-502-5P	98.77	66.51	906

Literature-anchored findings (GeneRIF, showing 1)

• LncFALEC recruits ART5/PARP1 and promotes castration-resistant prostate cancer through enhancing PARP1-meditated self PARylation. (PMID:36913068)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	si:ch211-145b13.6	ENSDARG00000069998
mus_musculus	Art5	ENSMUSG00000070424
rattus_norvegicus	Art5	ENSRNOG00000020242

Paralogs (3): ART4 (ENSG00000111339), ART1 (ENSG00000129744), ART3 (ENSG00000156219)

Protein

Protein identifiers

Ecto-ADP-ribosyltransferase 5 — Q96L15 (reviewed: Q96L15)

Alternative names: ADP-ribosyltransferase C2 and C3 toxin-like 5, Mono(ADP-ribosyl)transferase 5, NAD(P)(+)–arginine ADP-ribosyltransferase 5

All UniProt accessions (3): Q96L15, H7C1W0, H7C3U2

UniProt curated annotations — full annotation on UniProt →

Subcellular location. Secreted.

Similarity. Belongs to the Arg-specific ADP-ribosyltransferase family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q96L15-1	1	yes
Q96L15-2	2

RefSeq proteins (3): NP_001073004, NP_001284597, NP_443750* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000768	ART	Family
IPR050999	ADP-ribosyltransferase_ARG	Family

Pfam: PF01129

Catalyzed reactions (Rhea), 1 shown:

• L-arginyl-[protein] + NAD(+) = N(omega)-(ADP-D-ribosyl)-L-arginyl-[protein] + nicotinamide + H(+) (RHEA:19149)

UniProt features (18 total): binding site 4, glycosylation site 3, active site 3, splice variant 2, signal peptide 1, chain 1, disulfide bond 1, sequence variant 1, sequence conflict 1, domain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q96L15-F1	89.07	0.83

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Catalytic / active sites (3): 160; 183; 221

Ligand- & substrate-binding residues (4): 99; 160; 180; 214

Disulfide bonds (1): 42–258

Glycosylation sites (3): 101, 196, 250

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 62 (showing top): DARWICHE_SKIN_TUMOR_PROMOTER_UP, DARWICHE_PAPILLOMA_RISK_LOW_UP, DARWICHE_PAPILLOMA_RISK_HIGH_UP, DARWICHE_SQUAMOUS_CELL_CARCINOMA_DN, MEF2_02, MEF2_Q6_01, CTAWWWATA_RSRFC4_Q2, MEF2_03, GOMF_GLYCOSYLTRANSFERASE_ACTIVITY, GOMF_PENTOSYLTRANSFERASE_ACTIVITY, GOMF_TRANSFERASE_ACTIVITY_TRANSFERRING_PHOSPHORUS_CONTAINING_GROUPS, MIKKELSEN_IPS_LCP_WITH_H3K4ME3, MIKKELSEN_ES_LCP_WITH_H3K4ME3, MARTENS_TRETINOIN_RESPONSE_UP, BRIDEAU_IMPRINTED_GENES

GO Biological Process (0):

GO Molecular Function (6): NAD+ poly-ADP-ribosyltransferase activity (GO:0003950), nucleotidyltransferase activity (GO:0016779), NAD+-protein-arginine ADP-ribosyltransferase activity (GO:0106274), protein binding (GO:0005515), transferase activity (GO:0016740), glycosyltransferase activity (GO:0016757)

GO Cellular Component (1): extracellular region (GO:0005576)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
pentosyltransferase activity	1
transferase activity, transferring phosphorus-containing groups	1
NAD+-protein mono-ADP-ribosyltransferase activity	1
binding	1
catalytic activity	1
transferase activity	1
cellular anatomical structure	1

Protein interactions and networks

STRING

384 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
ART5	PARG	Q86W56	881
ART5	ADPRH	P54922	616
ART5	MACROD1	Q9BQ69	575
ART5	MACROD2	A1Z1Q3	543
ART5	PARP15	Q460N3	488
ART5	ITGA7	Q13683	450
ART5	PARP6	Q2NL67	431
ART5	ZDBF2	Q9HCK1	429
ART5	PARP10	Q53GL7	426
ART5	PARP8	Q8N3A8	417
ART5	CTXND1	A0A1B0GTU2	413
ART5	TRIM72	Q6ZMU5	411
ART5	ADPRS	Q9NX46	404
ART5	CHRNA10	Q9GZZ6	401
ART5	PARP9	Q8IXQ6	401

IntAct

58 interactions, top by confidence:

A	B	Type	Score
ART5	PGRMC2	psi-mi:“MI:0915”(physical association)	0.560
C1orf54	EXTL3	psi-mi:“MI:0914”(association)	0.530
ATG16L1		psi-mi:“MI:0914”(association)	0.350
KLK1	CRLF1	psi-mi:“MI:0914”(association)	0.350
MFAP4	CRLF1	psi-mi:“MI:0914”(association)	0.350
PDGFRA	GXYLT2	psi-mi:“MI:0914”(association)	0.350
CLEC12B	GXYLT2	psi-mi:“MI:0914”(association)	0.350
RLN1	RTL8C	psi-mi:“MI:0914”(association)	0.350
SCGB2A2	RTL8C	psi-mi:“MI:0914”(association)	0.350
CST9L	QSOX1	psi-mi:“MI:0914”(association)	0.350
LCN6	COCH	psi-mi:“MI:0914”(association)	0.350
SPX	ERI3	psi-mi:“MI:0914”(association)	0.350
PON2	ENTPD6	psi-mi:“MI:0914”(association)	0.350
LCN9	C1QL1	psi-mi:“MI:0914”(association)	0.350
BTNL2	TMEM131L	psi-mi:“MI:0914”(association)	0.350
SFTPC	TMEM131L	psi-mi:“MI:0914”(association)	0.350
LY86	TMEM131L	psi-mi:“MI:0914”(association)	0.350
IL5RA	POTEF	psi-mi:“MI:0914”(association)	0.350
ALPG	POTEF	psi-mi:“MI:0914”(association)	0.350
LYZL2	POTEF	psi-mi:“MI:0914”(association)	0.350
CFC1	POTEF	psi-mi:“MI:0914”(association)	0.350
IGLL5	POTEF	psi-mi:“MI:0914”(association)	0.350
ISLR		psi-mi:“MI:0914”(association)	0.350
NAAA	HAX1	psi-mi:“MI:0914”(association)	0.350
MFAP4	QSOX1	psi-mi:“MI:0914”(association)	0.350
DPEP2	QSOX1	psi-mi:“MI:0914”(association)	0.350
PDGFRA	QSOX1	psi-mi:“MI:0914”(association)	0.350
ELSPBP1	QSOX1	psi-mi:“MI:0914”(association)	0.350
PRG2	QSOX1	psi-mi:“MI:0914”(association)	0.350
DNASE1L1	QSOX1	psi-mi:“MI:0914”(association)	0.350

BioGRID (51): ART5 (Affinity Capture-MS), ART5 (Two-hybrid), ART5 (Affinity Capture-MS), ART5 (Affinity Capture-MS), ART5 (Affinity Capture-MS), ART5 (Affinity Capture-MS), ART5 (Affinity Capture-MS), ART5 (Affinity Capture-MS), ART5 (Affinity Capture-MS), ART5 (Affinity Capture-MS), ART5 (Affinity Capture-MS), ART5 (Affinity Capture-MS), ART5 (Affinity Capture-MS), ART5 (Affinity Capture-MS), ART5 (Affinity Capture-MS)

ESM2 similar proteins: D4AB34, G3V8H4, G7PWZ3, O08717, O88839, O88959, P17490, P17981, P17982, P20974, P43021, P52961, P55806, P55807, P58166, P70352, Q03515, Q04997, Q0V8J4, Q0VC22, Q13444, Q16671, Q28616, Q2KJ51, Q2TNK5, Q49KI5, Q5XHY4, Q60935, Q62893, Q6MG64, Q76MJ5, Q80XL1, Q8IZF5, Q8K592, Q92080, Q93070, Q95NE0, Q96L15, Q96S42, Q99988

Diamond homologs: O35975, P17981, P17982, P20974, P52961, P55806, P55807, P70352, Q03515, Q0VC22, Q13508, Q5XHY4, Q60935, Q8R2G4, Q92080, Q93070, Q95NE0, Q96L15, Q9CRA0

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 78 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Metabolism of carbohydrates and carbohydrate derivatives	5	12.3×	7e-03
Neutrophil degranulation	9	4.2×	9e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

66 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	53
Likely benign	3
Benign	2

Top pathogenic / likely-pathogenic (0)

SpliceAI

664 predictions. Top by Δscore:

Variant	Effect	Δscore
11:3639991:G:GA	donor_gain	0.9900
11:3640104:C:CA	donor_gain	0.9900
11:3640262:T:TA	donor_gain	0.9900
11:3640367:TGGGC:T	acceptor_gain	0.9900
11:3640368:GGGCC:G	acceptor_gain	0.9900
11:3640370:GC:G	acceptor_gain	0.9900
11:3640371:CC:C	acceptor_gain	0.9900
11:3640372:C:CA	acceptor_loss	0.9900
11:3640372:C:CC	acceptor_gain	0.9900
11:3640373:T:C	acceptor_loss	0.9900
11:3640380:C:CT	acceptor_gain	0.9900
11:3640381:A:T	acceptor_gain	0.9900
11:3641801:GTTA:G	donor_loss	0.9900
11:3641802:TTAC:T	donor_loss	0.9900
11:3641803:TA:T	donor_loss	0.9900
11:3641804:A:AT	donor_loss	0.9900
11:3641805:CCT:C	donor_loss	0.9900
11:3642079:T:TA	donor_gain	0.9900
11:3642107:T:A	donor_gain	0.9900
11:3642176:A:AC	donor_gain	0.9900
11:3642177:C:CC	donor_gain	0.9900
11:3640368:GGGC:G	acceptor_gain	0.9800
11:3641808:G:A	donor_gain	0.9800
11:3642084:AGAG:A	donor_gain	0.9800
11:3639957:C:CT	donor_gain	0.9700
11:3640369:GGC:G	acceptor_gain	0.9700
11:3640369:GGCC:G	acceptor_gain	0.9700
11:3640370:GCCTG:G	acceptor_gain	0.9700
11:3640374:G:C	acceptor_gain	0.9700
11:3640374:G:GC	acceptor_gain	0.9700

AlphaMissense

1880 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
11:3639877:G:C	S184R	0.978
11:3639877:G:T	S184R	0.978
11:3639879:T:G	S184R	0.978
11:3639847:A:C	F194L	0.974
11:3639847:A:T	F194L	0.974
11:3639849:A:G	F194L	0.974
11:3639739:A:C	F230L	0.972
11:3639739:A:T	F230L	0.972
11:3639741:A:G	F230L	0.972
11:3640036:C:A	K131N	0.967
11:3640036:C:G	K131N	0.967
11:3640024:G:C	F135L	0.966
11:3640024:G:T	F135L	0.966
11:3640026:A:G	F135L	0.966
11:3639661:A:C	F256L	0.961
11:3639661:A:T	F256L	0.961
11:3639663:A:G	F256L	0.961
11:3640045:A:C	F128L	0.957
11:3640045:A:T	F128L	0.957
11:3640047:A:G	F128L	0.957
11:3639886:A:C	F181L	0.955
11:3639886:A:T	F181L	0.955
11:3639888:A:G	F181L	0.955
11:3640039:G:C	F130L	0.955
11:3640039:G:T	F130L	0.955
11:3640041:A:G	F130L	0.955
11:3640225:C:A	W68C	0.951
11:3640225:C:G	W68C	0.951
11:3640324:A:C	F35L	0.951
11:3640324:A:T	F35L	0.951

dbSNP variants (sampled 300 via entrez): RS1000176092 (11:3640829 C>T), RS1000611646 (11:3641035 G>A,T), RS1001035551 (11:3638999 T>C), RS1001514578 (11:3643931 A>G,T), RS1001559551 (11:3643645 G>A), RS1002004094 (11:3639608 A>C,G), RS1002246842 (11:3643230 A>G,T), RS1002249090 (11:3643529 AAAGAAAAAAG>A), RS1002746217 (11:3642050 A>G), RS1003174851 (11:3644327 G>C,T), RS1003522827 (11:3642444 A>G), RS1003963670 (11:3642531 C>A), RS1004981603 (11:3642296 C>A,G,T), RS1005014646 (11:3642401 C>T), RS1005149872 (11:3644298 A>G)

Disease associations

OMIM: gene MIM:610625 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

3 associations (top):

Study	Trait	p-value
GCST010725_20	Malaria	4.000000e-69
GCST010725_33	Malaria	2.000000e-67
GCST010725_51	Malaria	1.000000e-55

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

19 total (human), top 19 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Benzo(a)pyrene	affects methylation, increases methylation	2
Phenylmercuric Acetate	affects cotreatment, decreases expression	2
Valproic Acid	affects expression, increases methylation	2
di-n-octyl phthalate	increases methylation	1
decabromobiphenyl ether	affects expression	1
butyraldehyde	increases expression	1
benzo(e)pyrene	increases methylation	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	decreases expression, affects cotreatment	1
dorsomorphin	affects cotreatment, decreases expression	1
(+)-JQ1 compound	increases expression	1
Sunitinib	decreases expression	1
Carbamazepine	affects expression	1
Cytarabine	decreases expression	1
Diazinon	increases methylation	1
Estradiol	decreases expression	1
Methapyrilene	increases methylation	1
Silicon Dioxide	affects expression	1
Cadmium Chloride	decreases expression	1
Copper Sulfate	decreases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.