ATP4B

Summary

ATP4B (ATPase H+/K+ transporting subunit beta, HGNC:820) is a protein-coding gene on chromosome 13q34, encoding Potassium-transporting ATPase subunit beta (P51164). The beta subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells.

The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase.

Source: NCBI Gene 496 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 63 total — 1 pathogenic
• Druggable target: yes — 5 molecules with ChEMBL bioactivity
• MANE Select transcript: NM_000705

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:820
Approved symbol	ATP4B
Name	ATPase H+/K+ transporting subunit beta
Location	13q34
Locus type	gene with protein product
Status	Approved
Aliases	ATP6B
Ensembl gene	ENSG00000186009
Ensembl biotype	protein_coding
OMIM	137217
Entrez	496

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 protein_coding

ENST00000335288

RefSeq mRNA: 1 — MANE Select: NM_000705 NM_000705

CCDS: CCDS9539

Canonical transcript exons

ENST00000335288 — 7 exons

Exon	Start	End
ENSE00001335684	113650406	113650507
ENSE00001335687	113651671	113651727
ENSE00001335690	113652873	113653072
ENSE00001335693	113653321	113653434
ENSE00001335697	113654814	113654942
ENSE00001335698	113648804	113649535
ENSE00001335703	113658033	113658198

Expression profiles

Bgee: expression breadth broad, 93 present calls, max score 88.30.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 0.2254 / max 410.1016, expressed in 1 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
138364	0.2254	1

Top tissues by expression

121 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
body of stomach	UBERON:0001161	88.30	gold quality
stomach	UBERON:0000945	87.14	gold quality
fundus of stomach	UBERON:0001160	80.95	gold quality
right uterine tube	UBERON:0001302	73.75	gold quality
fallopian tube	UBERON:0003889	60.32	gold quality
right lobe of thyroid gland	UBERON:0001119	54.32	gold quality
left lobe of thyroid gland	UBERON:0001120	53.96	gold quality
thyroid gland	UBERON:0002046	53.47	gold quality
spleen	UBERON:0002106	52.04	gold quality
adult mammalian kidney	UBERON:0000082	50.62	gold quality
endocervix	UBERON:0000458	48.78	gold quality
hypothalamus	UBERON:0001898	48.72	gold quality
kidney	UBERON:0002113	47.25	gold quality
right lung	UBERON:0002167	46.85	gold quality
cortex of kidney	UBERON:0001225	46.20	gold quality
Ammon’s horn	UBERON:0001954	44.74	gold quality
right coronary artery	UBERON:0001625	44.22	gold quality
right adrenal gland	UBERON:0001233	42.85	gold quality
lower esophagus mucosa	UBERON:0035834	42.81	silver quality
body of pancreas	UBERON:0001150	42.58	gold quality
uterine cervix	UBERON:0000002	42.46	gold quality
left uterine tube	UBERON:0001303	41.72	gold quality
ectocervix	UBERON:0012249	41.45	silver quality
right lobe of liver	UBERON:0001114	41.42	silver quality
temporal lobe	UBERON:0001871	41.40	gold quality
colonic epithelium	UBERON:0000397	41.34	gold quality
amygdala	UBERON:0001876	41.18	gold quality
right adrenal gland cortex	UBERON:0035827	40.89	gold quality
esophagus mucosa	UBERON:0002469	40.49	gold quality
caudate nucleus	UBERON:0001873	39.92	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	1.46

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

39 targeting ATP4B, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-1277-5P	100.00	73.95	5056
HSA-MIR-497-5P	99.92	71.83	2674
HSA-MIR-15A-5P	99.90	72.80	2787
HSA-MIR-15B-5P	99.90	72.78	2798
HSA-MIR-16-5P	99.90	72.80	2780
HSA-MIR-195-5P	99.90	72.81	2805
HSA-MIR-548E-5P	99.89	72.73	4486
HSA-MIR-424-5P	99.89	71.90	2641
HSA-MIR-6838-5P	99.89	71.94	2690
HSA-MIR-6857-5P	99.87	65.32	985
HSA-MIR-629-3P	99.85	67.99	1875
HSA-MIR-4447	99.85	67.81	2900
HSA-MIR-4713-5P	99.78	67.80	1794
HSA-MIR-7856-5P	99.75	69.99	2901
HSA-MIR-148A-3P	99.74	73.77	1700
HSA-MIR-148B-3P	99.74	73.75	1700
HSA-MIR-152-3P	99.74	73.75	1703
HSA-MIR-2116-3P	99.74	64.32	889
HSA-MIR-3618	99.69	68.57	1012
HSA-MIR-451B	99.55	68.28	1380
HSA-MIR-3616-5P	99.55	67.02	989
HSA-MIR-573	99.55	67.44	955
HSA-MIR-7159-5P	99.53	72.12	2472
HSA-MIR-5007-3P	99.51	68.14	1242
HSA-MIR-548G-3P	99.48	68.67	2159
HSA-MIR-1244	99.33	68.38	832
HSA-MIR-3191-5P	99.24	66.52	1722
HSA-MIR-4477B	99.23	70.49	1733
HSA-MIR-328-5P	99.08	64.65	1000
HSA-MIR-513B-3P	98.76	68.12	1577

Literature-anchored findings (GeneRIF, showing 5)

• Content of the beta1 subunit of sodium potassium pump in resistance trained control subjects was 33% higher in trained compared to untrained leg, and 47% higher in trained compared to untrained leg in diabetics. (PMID:14685860)
• Thus prolonged exhaustive exercise impaired each of the maximal in vitro Na+-K+-ATPase activity, Ca2+ release, and Ca2+ uptake rates. (PMID:15155714)
• Use of fold recognition methods enables the prediction that a C-terminal domain of the beta subunits of Na,K-ATPase and H,K-ATPase has an immunoglobulin-like fold, which resembles cell adhesion molecules. (PMID:19694409)
• Downregulation of ATP4B gene is associated with gastric cancer. (PMID:23317218)
• these findings demonstrated that a decrease in pHi, caused by H+/K+-ATPase inhibition induced by BMT-1, triggered the dysfunction of the mitochondria resulting in the apoptosis of MM cells (PMID:24700195)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
mus_musculus	Atp4b	ENSMUSG00000031449
rattus_norvegicus	Atp4b	ENSRNOG00000018543
drosophila_melanogaster	CG33310	FBGN0053310

Paralogs (4): ATP1B3 (ENSG00000069849), ATP1B4 (ENSG00000101892), ATP1B2 (ENSG00000129244), ATP1B1 (ENSG00000143153)

Protein

Protein identifiers

Potassium-transporting ATPase subunit beta — P51164 (reviewed: P51164)

Alternative names: Gastric H(+)/K(+) ATPase subunit beta, Proton pump beta chain

All UniProt accessions (1): P51164

UniProt curated annotations — full annotation on UniProt →

Function. The beta subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Plays a structural and regulatory role in the assembly and membrane targeting of a functionally active pump. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation of the alpha subunit that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). Interacts with the phosphorylation domain of the alpha subunit and functions as a ratchet, stabilizing the lumenal-open E2 conformation and preventing the reverse reaction of the transport cycle.

Subunit / interactions. The ATPase pump is composed of two subunits: alpha (catalytic) and beta (regulatory). Interacts with alpha subunit ATP12A; this interaction is required for the formation of a functionally active pump and targeting at the plasma membrane. Interacts (via N-terminus) with alpha subunit ATP4A (via the P-domain).

Subcellular location. Apical cell membrane. Cell membrane.

Post-translational modifications. N-glycosylation is necessary for assembly and functional expression of the pump at the plasma membrane.

Domain organisation. The C-terminal lobe folds into an immunoglobulin-like domain and mediates cell adhesion properties.

Similarity. Belongs to the X(+)/potassium ATPases subunit beta family.

RefSeq proteins (1): NP_000696* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000402	Na/K_ATPase_sub_beta	Family
IPR038702	Na/K_ATPase_sub_beta_sf	Homologous_superfamily

Pfam: PF00287

UniProt features (15 total): glycosylation site 7, disulfide bond 3, topological domain 2, chain 1, transmembrane region 1, region of interest 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-P51164-F1	90.68	0.76

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Disulfide bonds (3): 131–152, 162–178, 201–263

Glycosylation sites (7): 193, 222, 99, 103, 130, 146, 161

Function

Pathways and Gene Ontology

Reactome pathways

3 pathways

ID	Pathway
R-HSA-936837	Ion transport by P-type ATPases
R-HSA-382551	Transport of small molecules
R-HSA-983712	Ion channel transport

MSigDB gene sets: 131 (showing top): GOBP_POTASSIUM_ION_TRANSPORT, GOBP_SODIUM_ION_TRANSMEMBRANE_TRANSPORT, GOBP_POTASSIUM_ION_HOMEOSTASIS, MORF_RAD51L3, GOBP_MONOATOMIC_CATION_TRANSPORT, MORF_CTSB, chr13q34, MORF_IL4, GOBP_INTRACELLULAR_POTASSIUM_ION_HOMEOSTASIS, MORF_PRKCA, GOBP_INTRACELLULAR_SODIUM_ION_HOMEOSTASIS, GOBP_RESPONSE_TO_OXYGEN_CONTAINING_COMPOUND, GOBP_SODIUM_ION_HOMEOSTASIS, GOBP_RESPONSE_TO_LIPID, GOMF_PROTON_TRANSMEMBRANE_TRANSPORTER_ACTIVITY

GO Biological Process (12): intracellular sodium ion homeostasis (GO:0006883), cell adhesion (GO:0007155), intracellular potassium ion homeostasis (GO:0030007), response to lipopolysaccharide (GO:0032496), sodium ion export across plasma membrane (GO:0036376), pH reduction (GO:0045851), potassium ion transmembrane transport (GO:0071805), potassium ion import across plasma membrane (GO:1990573), monoatomic ion transport (GO:0006811), potassium ion transport (GO:0006813), sodium ion transport (GO:0006814), proton transmembrane transport (GO:1902600)

GO Molecular Function (4): ATPase activator activity (GO:0001671), P-type potassium:proton transporter activity (GO:0008900), heterocyclic compound binding (GO:1901363), protein binding (GO:0005515)

GO Cellular Component (5): plasma membrane (GO:0005886), potassium:proton exchanging ATPase complex (GO:0005889), sodium:potassium-exchanging ATPase complex (GO:0005890), apical plasma membrane (GO:0016324), membrane (GO:0016020)

Reactome top-level categories

Rollup of top-2 pathways:

Category	Pathways
Ion channel transport	1
Transport of small molecules	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
intracellular monoatomic cation homeostasis	2
monoatomic cation transmembrane transport	2
metal ion transport	2
cation-transporting ATPase complex	2
plasma membrane protein complex	2
sodium ion homeostasis	1
cellular process	1
potassium ion homeostasis	1
response to molecule of bacterial origin	1
response to lipid	1
response to oxygen-containing compound	1
sodium ion transmembrane transport	1
export across plasma membrane	1
regulation of pH	1
potassium ion transport	1
potassium ion transmembrane transport	1
inorganic cation import across plasma membrane	1
transport	1
ATP-dependent activity	1
molecular function activator activity	1
P-type proton-exporting transporter activity	1
P-type potassium transmembrane transporter activity	1
small molecule binding	1
binding	1
membrane	1
cell periphery	1
apical part of cell	1
plasma membrane region	1
cellular anatomical structure	1

Protein interactions and networks

STRING

786 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
ATP4B	ATP4A	P20648	822
ATP4B	ATP12A	P54707	808
ATP4B	ATP7B	P35670	731
ATP4B	CBLIF	P27352	676
ATP4B	CLTC	Q00610	582
ATP4B	PGC	P20142	543
ATP4B	TFF2	Q03403	520
ATP4B	PRKCA	P17252	493
ATP4B	GAST	P01350	492
ATP4B	MUC6	Q6W4X9	461
ATP4B	GKN1	Q9NS71	446
ATP4B	SLC7A1	P30825	436
ATP4B	CP	P00450	425
ATP4B	MUC5AC	P98088	418
ATP4B	CAPN8	A6NHC0	386

IntAct

13 interactions, top by confidence:

A	B	Type	Score
CCNDBP1	ATP4B	psi-mi:“MI:0915”(physical association)	0.560
ATP4B	CCNDBP1	psi-mi:“MI:0915”(physical association)	0.560
ATP4B	ZC4H2	psi-mi:“MI:0915”(physical association)	0.560
GPR42	ATP4B	psi-mi:“MI:0915”(physical association)	0.560
ATP4B	CCT7	psi-mi:“MI:0915”(physical association)	0.400
BPGM	ATP4B	psi-mi:“MI:0915”(physical association)	0.370
AGO3	ATP4B	psi-mi:“MI:0915”(physical association)	0.370
ATP4B	ZC4H2	psi-mi:“MI:0915”(physical association)	0.000
ATP4B	GPR42	psi-mi:“MI:0915”(physical association)	0.000

BioGRID (8): CCNDBP1 (Two-hybrid), ATP4B (Two-hybrid), ATP4B (Two-hybrid), ATP4B (Two-hybrid), CCT7 (Proximity Label-MS), ATP4B (Affinity Capture-MS), AGO3 (Two-hybrid), BPGM (Two-hybrid)

ESM2 similar proteins: A5A6J8, P05026, P05027, P05028, P05029, P06583, P07340, P08251, P13638, P14094, P14231, P14415, P18434, P18597, P18598, P21188, P30715, P30716, P33704, P33879, P43002, P50992, P51164, P51165, P51575, P51577, P54709, Q17QL5, Q202B1, Q24048, Q28030, Q2HZ96, Q4R4V5, Q5E9U1, Q5F362, Q5J583, Q5R6C0, Q5R8S8, Q6AY41, Q8L8W0

Diamond homologs: A5A6J8, A7MB71, P05026, P05027, P05028, P05029, P06583, P07340, P08251, P13638, P14094, P14231, P14415, P18434, P18597, P18598, P21188, P30715, P30716, P33704, P33879, P43002, P50992, P51164, P51165, P54709, P97370, Q202B1, Q24046, Q28030, Q2HZ96, Q3T0C6, Q4R4V5, Q5J583, Q5R8S8, Q60489, Q63377, Q8WMG3, Q99ME6, Q9BDK6

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

63 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	1
Likely pathogenic	0
Uncertain significance	52
Likely benign	6
Benign	0

Top pathogenic / likely-pathogenic (1)

Variant ID	HGVS	Classification
4279224	GRCh37/hg19 13q33.1-34(chr13:104324715-115107733)x1	Pathogenic

SpliceAI

1643 predictions. Top by Δscore:

Variant	Effect	Δscore
13:113649531:TGGGG:T	acceptor_gain	1.0000
13:113649532:GGGG:G	acceptor_gain	1.0000
13:113649534:GG:G	acceptor_gain	1.0000
13:113649536:C:CC	acceptor_gain	1.0000
13:113649536:C:CG	acceptor_loss	1.0000
13:113649541:G:C	acceptor_gain	1.0000
13:113649541:G:GC	acceptor_gain	1.0000
13:113649547:G:GC	acceptor_gain	1.0000
13:113650401:CCTA:C	donor_loss	1.0000
13:113650402:CTA:C	donor_loss	1.0000
13:113650403:TACC:T	donor_loss	1.0000
13:113650404:A:C	donor_loss	1.0000
13:113652903:T:TA	donor_gain	1.0000
13:113653834:A:C	donor_gain	1.0000
13:113654808:GCTTA:G	donor_loss	1.0000
13:113654809:CTTA:C	donor_loss	1.0000
13:113654810:TTA:T	donor_loss	1.0000
13:113654811:TA:T	donor_loss	1.0000
13:113654813:C:CT	donor_loss	1.0000
13:113654938:CCACA:C	acceptor_gain	1.0000
13:113654939:CACA:C	acceptor_gain	1.0000
13:113654939:CACAC:C	acceptor_gain	1.0000
13:113654940:ACA:A	acceptor_gain	1.0000
13:113654941:CA:C	acceptor_gain	1.0000
13:113654941:CAC:C	acceptor_gain	1.0000
13:113654942:ACT:A	acceptor_loss	1.0000
13:113654943:C:CA	acceptor_loss	1.0000
13:113654943:C:CC	acceptor_gain	1.0000
13:113654944:T:G	acceptor_loss	1.0000
13:113654947:G:T	acceptor_gain	1.0000

AlphaMissense

1927 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
13:113649462:C:G	C263S	0.999
13:113649463:A:T	C263S	0.999
13:113649463:A:G	C263R	0.998
13:113652973:C:G	C152S	0.998
13:113652974:A:T	C152S	0.998
13:113654932:G:C	S41R	0.998
13:113654932:G:T	S41R	0.998
13:113654934:T:G	S41R	0.998
13:113649396:A:G	F285S	0.997
13:113649462:C:T	C263Y	0.997
13:113652966:G:C	F154L	0.997
13:113652966:G:T	F154L	0.997
13:113652968:A:G	F154L	0.997
13:113651681:C:G	C201S	0.996
13:113651682:A:T	C201S	0.996
13:113652973:C:T	C152Y	0.996
13:113649409:C:A	G281W	0.995
13:113649461:G:C	C263W	0.995
13:113650430:G:C	F230L	0.995
13:113650430:G:T	F230L	0.995
13:113650432:A:G	F230L	0.995
13:113651681:C:T	C201Y	0.995
13:113652876:G:C	N184K	0.995
13:113652876:G:T	N184K	0.995
13:113652967:A:C	F154C	0.995
13:113652973:C:A	C152F	0.995
13:113650431:A:C	F230C	0.994
13:113651680:G:C	C201W	0.994
13:113652943:C:G	C162S	0.994
13:113652944:A:T	C162S	0.994

dbSNP variants (sampled 300 via entrez): RS1000017906 (13:113651392 C>A), RS1000160201 (13:113650066 G>C), RS1000400109 (13:113657380 C>T), RS1000439952 (13:113649203 G>A), RS1000660976 (13:113650295 G>A,T), RS1000721186 (13:113658290 G>A), RS1001010245 (13:113649987 A>T), RS1001230708 (13:113653258 A>C,G), RS1001231400 (13:113657185 G>T), RS1001611241 (13:113648738 G>A), RS1001754324 (13:113648433 T>C), RS1002063433 (13:113649117 G>A,T), RS1002122590 (13:113657566 G>A,T), RS1002725411 (13:113656755 A>G), RS1002829903 (13:113652442 C>G,T)

Disease associations

OMIM: gene MIM:137217 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST008839_511	Height	1.000000e-10

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL2095173 (PROTEIN COMPLEX)

Molecules with ChEMBL bioactivity

5 molecules (phase ≥1), by development phase (incl. off-target/promiscuous compounds). Patent mentions across the top 20 by phase: 169,080 (via chembl_molecule»patent_compound — counts attach to the compound, not the gene–compound relationship, so off-target/promiscuous molecules can dominate).

Molecule	Name	Phase	Patents
CHEMBL1502	PANTOPRAZOLE	4	14,689
CHEMBL1503	OMEPRAZOLE	4	52,284
CHEMBL1790041	RANITIDINE	4	30,599
CHEMBL30	CIMETIDINE	4	47,191
CHEMBL480	LANSOPRAZOLE	4	24,317

PharmGKB: 1 entry (VIP=true, CPIC=false)

GtoPdb / IUPHAR curated pharmacology

(IUPHAR/BPS Guide to Pharmacology — expert-curated)

Target class: transporter — H⁺/K⁺-ATPases

ChEMBL bioactivities

249 potent at pChembl≥5 of 306 total, top 50 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
7.70	IC50	20	nM	CHEMBL1202672
7.52	IC50	30	nM	CHEMBL1202661
7.40	IC50	40	nM	RANITIDINE
7.30	IC50	50	nM	CHEMBL1202647
7.30	IC50	50	nM	CHEMBL1202621
7.19	IC50	64	nM	CHEMBL296482
7.19	IC50	65	nM	CHEMBL47529
7.10	IC50	80	nM	CHEMBL1202681
7.10	IC50	80	nM	CHEMBL1202672
7.10	IC50	80	nM	CHEMBL1202647
7.05	IC50	90	nM	CHEMBL1202667
6.96	IC50	110	nM	CHEMBL1202658
6.96	IC50	110	nM	CHEMBL1202621
6.92	IC50	120	nM	CHEMBL1202681
6.92	IC50	120	nM	CHEMBL1202661
6.92	IC50	120	nM	CHEMBL1202663
6.92	IC50	120	nM	CHEMBL1202667
6.89	IC50	130	nM	CHEMBL1202663
6.89	IC50	130	nM	CHEMBL1202659
6.89	IC50	130	nM	CHEMBL293244
6.85	IC50	140	nM	CHEMBL1202607
6.85	IC50	140	nM	CHEMBL1202656
6.82	IC50	150	nM	CHEMBL1202651
6.82	IC50	150	nM	CHEMBL1202654
6.80	IC50	160	nM	CHEMBL1202629
6.77	IC50	170	nM	CHEMBL1202678
6.77	IC50	170	nM	CHEMBL1202671
6.75	IC50	180	nM	CHEMBL1202660
6.75	IC50	180	nM	CHEMBL1202679
6.75	IC50	180	nM	CHEMBL1202655
6.75	IC50	180	nM	CHEMBL1202668
6.70	IC50	200	nM	CHEMBL1202666
6.70	IC50	200	nM	CHEMBL20162
6.68	IC50	210	nM	CHEMBL1202607
6.68	IC50	210	nM	CHEMBL1202655
6.68	IC50	210	nM	CHEMBL1202656
6.66	IC50	220	nM	CHEMBL1202677
6.64	IC50	230	nM	CHEMBL1202642
6.64	IC50	230	nM	CHEMBL1202615
6.64	IC50	230	nM	CHEMBL1202668
6.64	IC50	230	nM	CHEMBL1202673
6.62	IC50	240	nM	CHEMBL1202675
6.62	IC50	240	nM	CHEMBL1202678
6.60	IC50	250	nM	OMEPRAZOLE
6.60	IC50	250	nM	CHEMBL1202643
6.60	IC50	250	nM	CHEMBL1202608
6.60	IC50	250	nM	CHEMBL1202638
6.58	IC50	260	nM	CHEMBL1202666
6.58	IC50	260	nM	CHEMBL1202643
6.58	IC50	260	nM	CHEMBL1202644

PubChem BioAssay actives

251 with measured affinity, of 493 total; 50 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-[4-(3-imidazol-1-ylpropoxy)phenyl]-2-phenyl-1,3-thiazole;hydrate;hydrochloride	167650: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by dibutyryl cyclic adenosine 3’, 5’ -monophosphate (dcAMP)	ic50	0.0200	uM
4-[4-[(E)-2-(1,3-benzothiazol-2-yl)ethenyl]phenoxy]-N,N-dipropylbutan-1-amine;hydrate;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.0300	uM
1-N’-[2-[[5-[(dimethylamino)methyl]furan-2-yl]methylsulfanyl]ethyl]-1-N-methyl-2-nitroethene-1,1-diamine	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.0400	uM
2-phenyl-4-[4-(3-piperidin-1-ylpropoxy)phenyl]-1,3-thiazole;hydrochloride	167650: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by dibutyryl cyclic adenosine 3’, 5’ -monophosphate (dcAMP)	ic50	0.0500	uM
N-butyl-N-[3-[4-[(E)-2-(6-methoxy-1,3-benzoxazol-2-yl)ethenyl]phenoxy]propyl]butan-1-amine;hydrochloride	167650: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by dibutyryl cyclic adenosine 3’, 5’ -monophosphate (dcAMP)	ic50	0.0500	uM
2-(4-methyl-12-phenyl-13-oxa-3,6-diazatricyclo[7.4.0.02,6]trideca-1(9),2,4,7-tetraen-5-yl)acetonitrile	78910: Inhibition of H+/K+ ATPase as reduced acid formation in rabbit gastric glands	ic50	0.0640	uM
2-(2-methyl-8-phenylmethoxyimidazo[1,2-a]pyridin-3-yl)acetonitrile	78910: Inhibition of H+/K+ ATPase as reduced acid formation in rabbit gastric glands	ic50	0.0650	uM
4-[4-[(E)-2-(1,3-benzoxazol-2-yl)ethenyl]phenoxy]-N,N-diethylbutan-1-amine;hydrate;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.0800	uM
N,N-diethyl-3-[4-(2-phenyl-1,3-thiazol-4-yl)phenoxy]propan-1-amine;hydrate;hydrochloride	167650: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by dibutyryl cyclic adenosine 3’, 5’ -monophosphate (dcAMP)	ic50	0.0900	uM
4-[4-[(E)-2-(1,3-benzoxazol-2-yl)ethenyl]phenoxy]-N,N-dipropylbutan-1-amine;hydrochloride	167650: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by dibutyryl cyclic adenosine 3’, 5’ -monophosphate (dcAMP)	ic50	0.1100	uM
N,N-dibutyl-4-[4-(2-phenyl-1,3-thiazol-4-yl)phenoxy]butan-1-amine;hydrochloride	167650: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by dibutyryl cyclic adenosine 3’, 5’ -monophosphate (dcAMP)	ic50	0.1200	uM
2-[2-methyl-8-(2-phenylethyl)imidazo[1,2-a]pyridin-3-yl]acetonitrile	78910: Inhibition of H+/K+ ATPase as reduced acid formation in rabbit gastric glands	ic50	0.1300	uM
2-[(E)-2-[4-(3-imidazol-1-ylpropoxy)phenyl]ethenyl]-1,3-benzothiazole;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.1300	uM
3-[4-[(E)-2-(1,3-benzothiazol-2-yl)ethenyl]phenoxy]-N,N-dipropylpropan-1-amine;hydrate;hydrochloride	167650: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by dibutyryl cyclic adenosine 3’, 5’ -monophosphate (dcAMP)	ic50	0.1400	uM
3-[4-(1,3-benzothiazol-2-yl)phenoxy]-N,N-dipropylpropan-1-amine;hydrate;hydrochloride	167650: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by dibutyryl cyclic adenosine 3’, 5’ -monophosphate (dcAMP)	ic50	0.1400	uM
N-[3-[4-[(E)-2-(1,3-benzoxazol-2-yl)ethenyl]phenoxy]propyl]-N-butylbutan-1-amine;hydrochloride	167650: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by dibutyryl cyclic adenosine 3’, 5’ -monophosphate (dcAMP)	ic50	0.1500	uM
3-[4-[(E)-2-(1,3-benzothiazol-2-yl)ethenyl]phenoxy]-N,N-diethylpropan-1-amine;hydrate;hydrochloride	167650: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by dibutyryl cyclic adenosine 3’, 5’ -monophosphate (dcAMP)	ic50	0.1500	uM
2-[(E)-2-[4-(4-pyrrolidin-1-ylbutoxy)phenyl]ethenyl]-1,3-benzothiazole;hydrate;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.1600	uM
2-[(E)-2-[4-(4-piperidin-1-ylbutoxy)phenyl]ethenyl]-1,3-benzoxazole;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.1700	uM
2-[(E)-2-[4-(4-pyrrolidin-1-ylbutoxy)phenyl]ethenyl]-1,3-benzoxazole;hydrate;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.1700	uM
2-[(E)-2-[4-(3-piperidin-1-ylpropoxy)phenyl]ethenyl]-1,3-benzothiazole;hydrate;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.1800	uM
4-[4-[(E)-2-(1,3-benzothiazol-2-yl)ethenyl]phenoxy]-N,N-diethylbutan-1-amine;hydrate;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.1800	uM
3-[4-(2-phenyl-1,3-thiazol-4-yl)phenoxy]-N,N-dipropylpropan-1-amine;hydrate;hydrochloride	167650: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by dibutyryl cyclic adenosine 3’, 5’ -monophosphate (dcAMP)	ic50	0.1800	uM
4-[4-[(E)-2-(1,3-benzoxazol-2-yl)ethenyl]phenoxy]-N,N-dibutylbutan-1-amine;hydrate;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.1800	uM
2-(1H-benzimidazol-2-ylsulfinylmethyl)-4-methoxy-3,5-dimethylaniline	78730: In vitro inhibitory activity against H+/K+ ATPase prepared from canine fundic mucosa	ic50	0.2000	uM
2-[(E)-2-[4-(4-imidazol-1-ylbutoxy)phenyl]ethenyl]-1,3-benzothiazole;hydrate;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.2000	uM
3-[4-[(E)-2-(1,3-benzoxazol-2-yl)ethenyl]phenoxy]-N,N-dipropylpropan-1-amine;hydrate;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.2200	uM
2-[(E)-2-[4-(5-imidazol-1-ylpentoxy)phenyl]ethenyl]-1,3-benzoxazole;hydrate;hydrochloride	167650: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by dibutyryl cyclic adenosine 3’, 5’ -monophosphate (dcAMP)	ic50	0.2300	uM
N-butyl-N-[3-[4-[2-(4-methoxyphenyl)-1,3-thiazol-4-yl]phenoxy]propyl]butan-1-amine;hydrate;hydrochloride	167650: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by dibutyryl cyclic adenosine 3’, 5’ -monophosphate (dcAMP)	ic50	0.2300	uM
N-butyl-N-[3-[4-(5-methyl-2-phenyl-1,3-thiazol-4-yl)phenoxy]propyl]butan-1-amine;hydrate;hydrochloride	167650: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by dibutyryl cyclic adenosine 3’, 5’ -monophosphate (dcAMP)	ic50	0.2300	uM
N-butyl-N-[3-[4-(5-ethyl-2-phenyl-1,3-thiazol-4-yl)phenoxy]propyl]butan-1-amine;hydrate;hydrochloride	167650: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by dibutyryl cyclic adenosine 3’, 5’ -monophosphate (dcAMP)	ic50	0.2400	uM
6-methoxy-2-[(4-methoxy-3,5-dimethyl-2-pyridinyl)methylsulfinyl]-1H-benzimidazole	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.2500	uM
2-[(E)-2-[4-(3-imidazol-1-ylpropoxy)phenyl]ethenyl]-1,3-benzoxazole;hydrate;hydrochloride	167650: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by dibutyryl cyclic adenosine 3’, 5’ -monophosphate (dcAMP)	ic50	0.2500	uM
N-[3-[4-(1,3-benzothiazol-2-yl)phenoxy]propyl]-N-butylbutan-1-amine;hydrate;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.2500	uM
N-[3-[4-[(E)-2-(1,3-benzothiazol-2-yl)ethenyl]phenoxy]propyl]-N-butylbutan-1-amine;hydrate;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.2500	uM
N-[2-[4-[(E)-2-(1,3-benzoxazol-2-yl)ethenyl]phenoxy]ethyl]-N-butylbutan-1-amine;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.2600	uM
N-butyl-N-[3-[4-[(E)-2-(5-methyl-1,3-benzoxazol-2-yl)ethenyl]phenoxy]propyl]butan-1-amine;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.2600	uM
N-butyl-N-[3-[4-[(E)-2-(6-methoxy-1,3-benzothiazol-2-yl)ethenyl]phenoxy]propyl]butan-1-amine;hydrate;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.2600	uM
N-butyl-N-[3-[4-[2-(3,5-dimethoxyphenyl)-1,3-thiazol-4-yl]phenoxy]propyl]butan-1-amine;hydrate;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.2600	uM
2-[(E)-2-[4-(4-imidazol-1-ylbutoxy)phenyl]ethenyl]-1,3-benzoxazole;hydrate;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.2700	uM
N-butyl-N-[3-[4-[(E)-2-(5-methyl-1,3-benzothiazol-2-yl)ethenyl]phenoxy]propyl]butan-1-amine;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.2900	uM
2-benzyl-1-[4-(3,4-dihydroxyphenyl)-1,3-thiazol-2-yl]guanidine;hydrochloride	78732: Inhibitory activity against dog gastric proton-pump enzyme H+/K+ ATPase	ic50	0.3000	uM
2-methyl-8-phenylmethoxyimidazo[1,2-a]pyridin-3-amine	78910: Inhibition of H+/K+ ATPase as reduced acid formation in rabbit gastric glands	ic50	0.3100	uM
N-butyl-N-[2-[4-(2-phenyl-1,3-thiazol-4-yl)phenoxy]ethyl]butan-1-amine;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.3100	uM
4-[4-(4-imidazol-1-ylbutoxy)phenyl]-2-phenyl-1,3-thiazole;hydrate;hydrochloride	167650: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by dibutyryl cyclic adenosine 3’, 5’ -monophosphate (dcAMP)	ic50	0.3100	uM
3-[4-[(E)-2-(1,3-benzoxazol-2-yl)ethenyl]phenoxy]-N,N-diethylpropan-1-amine;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.3200	uM
4-[4-(3-imidazol-1-ylpropoxy)phenyl]-2-[4-(trifluoromethyl)phenyl]-1,3-thiazole;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.3700	uM
2-phenyl-4-[4-(3-pyrrolidin-1-ylpropoxy)phenyl]-1,3-thiazole;hydrochloride	167652: Antisecretory activity evaluated by the inhibition of 14C -AP uptake in isolated rabbit parietal cells stimulated by exogenous histamine	ic50	0.3900	uM
2-[(4-methoxy-3-methyl-2-pyridinyl)methylsulfinyl]-6-(1,1,2,2-tetrafluoroethoxy)-1H-benzimidazole	78913: In vitro evaluation for the inhibition of H+/K+ ATPase at pH < 3 in the gastric glands of isolated rabbit stomach.	ic50	0.3981	uM
Lansoprazole	78913: In vitro evaluation for the inhibition of H+/K+ ATPase at pH < 3 in the gastric glands of isolated rabbit stomach.	ic50	0.3981	uM

CTD chemical–gene interactions

12 total (human), top 12 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
aristolochic acid I	increases expression	1
aflatoxin B2	decreases methylation	1
Sch 28080	affects cotreatment, decreases reaction, increases uptake	1
Resveratrol	affects cotreatment, decreases expression	1
Benzo(a)pyrene	affects methylation	1
Carmustine	decreases expression	1
Diazinon	increases methylation	1
Formaldehyde	decreases expression	1
Ouabain	affects cotreatment, decreases reaction, increases uptake	1
Plant Extracts	affects cotreatment, decreases expression	1
Rubidium	affects cotreatment, increases uptake, decreases reaction	1
Valproic Acid	increases methylation	1

ChEMBL screening assays

17 unique, capped per target: 13 binding, 4 functional

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL686427	Binding	Inhibitory activity against dog gastric proton-pump enzyme H+/K+ ATPase	Antiulcer agents. 4-substituted 2-guanidinothiazoles: reversible, competitive, and selective inhibitors of gastric H+,K(+)-ATPase. — J Med Chem
CHEMBL765030	Functional	Inhibition of [14C]aminopyrine (AP) accumulation stimulated by dibutyryl cyclic AMP in isolated rabbit parietal cells	Nicotinamide derivatives as a new class of gastric H+/K(+)-ATPase inhibitors. 1. Synthesis and structure-activity relationships of N-substituted 2-(benzhydryl- and benzylsulfinyl)nicotinamides. — J Med Chem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.