ATP5MF

Summary

ATP5MF (ATP synthase membrane subunit f, HGNC:848) is a protein-coding gene on chromosome 7q22.1, encoding ATP synthase F(0) complex subunit f, mitochondrial (P56134). Subunit f, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is a common-essential gene (DepMap: required in 99.7% of cancer cell lines).

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The catalytic portion of mitochondrial ATP synthase consists of five different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and single representatives of the gamma, delta, and epsilon subunits. The proton channel likely has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the f subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has multiple pseudogenes. Naturally occurring read-through transcription also exists between this gene and the downstream pentatricopeptide repeat domain 1 (PTCD1) gene.

Source: NCBI Gene 9551 — RefSeq curated summary.

At a glance

• GWAS associations: 7
• Clinical variants (ClinVar): 12 total
• Druggable target: yes
• Cancer dependency (DepMap): dependent in 99.7% of screened cell lines (common-essential)
• MANE Select transcript: NM_004889

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:848
Approved symbol	ATP5MF
Name	ATP synthase membrane subunit f
Location	7q22.1
Locus type	gene with protein product
Status	Approved
Aliases	ATP5JL
Ensembl gene	ENSG00000241468
Ensembl biotype	protein_coding
OMIM	619792
Entrez	9551

Gene structure

Transcript identifiers

Ensembl transcripts: 21 — 15 protein_coding, 4 retained_intron, 1 nonsense_mediated_decay, 1 protein_coding_CDS_not_defined

ENST00000292475, ENST00000359832, ENST00000394186, ENST00000414062, ENST00000449683, ENST00000466753, ENST00000481899, ENST00000485011, ENST00000488775, ENST00000491560, ENST00000523680, ENST00000524321, ENST00000889604, ENST00000889605, ENST00000889606, ENST00000915553, ENST00000915554, ENST00000915555, ENST00000915556, ENST00000915557, ENST00000956684

RefSeq mRNA: 4 — MANE Select: NM_004889 NM_001003713, NM_001003714, NM_001039178, NM_004889

CCDS: CCDS34692, CCDS47653, CCDS47654, CCDS5665

Canonical transcript exons

ENST00000292475 — 4 exons

Exon	Start	End
ENSE00003462758	99459147	99459263
ENSE00003663375	99460086	99460193
ENSE00003903070	99458195	99458355
ENSE00003904043	99466111	99466167

Expression profiles

Bgee: expression breadth ubiquitous, 295 present calls, max score 99.59.

FANTOM5 (CAGE): breadth broad, TPM avg 0.8358 / max 16.2662, expressed in 513 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
85107	206.6924	1828
85106	0.8358	513

Top tissues by expression

295 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
apex of heart	UBERON:0002098	99.59	gold quality
right testis	UBERON:0004534	99.56	gold quality
left testis	UBERON:0004533	99.55	gold quality
mucosa of transverse colon	UBERON:0004991	99.54	gold quality
endometrium epithelium	UBERON:0004811	99.51	gold quality
right frontal lobe	UBERON:0002810	99.50	gold quality
hindlimb stylopod muscle	UBERON:0004252	99.50	gold quality
right atrium auricular region	UBERON:0006631	99.47	gold quality
prefrontal cortex	UBERON:0000451	99.46	gold quality
heart left ventricle	UBERON:0002084	99.45	gold quality
metanephros cortex	UBERON:0010533	99.45	gold quality
ganglionic eminence	UBERON:0004023	99.44	gold quality
cardiac ventricle	UBERON:0002082	99.42	gold quality
adenohypophysis	UBERON:0002196	99.42	gold quality
right adrenal gland	UBERON:0001233	99.40	gold quality
gastrocnemius	UBERON:0001388	99.38	gold quality
left adrenal gland	UBERON:0001234	99.37	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	99.37	gold quality
left adrenal gland cortex	UBERON:0035825	99.37	gold quality
right adrenal gland cortex	UBERON:0035827	99.37	gold quality
islet of Langerhans	UBERON:0000006	99.36	gold quality
cortical plate	UBERON:0005343	99.36	gold quality
right hemisphere of cerebellum	UBERON:0014890	99.35	gold quality
rectum	UBERON:0001052	99.34	gold quality
pituitary gland	UBERON:0000007	99.33	gold quality
cingulate cortex	UBERON:0003027	99.33	gold quality
ventricular zone	UBERON:0003053	99.33	gold quality
heart	UBERON:0000948	99.32	gold quality
cardiac atrium	UBERON:0002081	99.31	gold quality
anterior cingulate cortex	UBERON:0009835	99.31	gold quality

Single-cell (SCXA)

Detected in 10 experiment(s), a significant marker in 7.

Experiment	Marker?	Max mean expression
E-GEOD-89232	yes	2186.27
E-HCAD-4	yes	142.21
E-MTAB-8410	yes	46.58
E-GEOD-84465	yes	26.51
E-MTAB-7316	yes	7.66
E-MTAB-10042	yes	7.43
E-MTAB-10596	no	1347.82
E-MTAB-7303	no	1216.04
E-GEOD-70580	no	607.78
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

9 targeting ATP5MF, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-651-5P	99.64	68.49	1104
HSA-MIR-488-3P	99.61	68.79	1731
HSA-MIR-4291	99.20	68.88	2969
HSA-MIR-4326	98.97	67.63	962
HSA-MIR-4664-5P	98.17	65.07	1020
HSA-MIR-342-5P	97.25	64.10	817
HSA-MIR-3201	97.16	65.42	1044
HSA-MIR-4791	96.51	67.76	659
HSA-MIR-891A-5P	93.24	65.86	104

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 99.7% of screened cell lines, common-essential.

Literature-anchored findings (GeneRIF, showing 2)

• The new role of F1Fo ATP synthase in mitochondria-mediated neurodegeneration and neuroprotection. (PMID:32653453)
• The F1Fo-ATPase inhibitor, IF1, is a critical regulator of energy metabolism in cancer cells. (PMID:33929490)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
mus_musculus	Atp5mf	ENSMUSG00000038690
drosophila_melanogaster	ATPsynF	FBGN0035032
caenorhabditis_elegans		WBGENE00011273

Protein

Protein identifiers

ATP synthase F(0) complex subunit f, mitochondrial — P56134 (reviewed: P56134)

Alternative names: ATP synthase membrane subunit f

All UniProt accessions (3): P56134, C9JU26, E5RKA0

UniProt curated annotations — full annotation on UniProt →

Function. Subunit f, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. Part of the complex F(0) domain.

Subunit / interactions. Component of the ATP synthase complex composed at least of ATP5F1A/subunit alpha, ATP5F1B/subunit beta, ATP5MC1/subunit c (homooctamer), MT-ATP6/subunit a, MT-ATP8/subunit 8, ATP5ME/subunit e, ATP5MF/subunit f, ATP5MG/subunit g, ATP5MK/subunit k, ATP5MJ/subunit j, ATP5F1C/subunit gamma, ATP5F1D/subunit delta, ATP5F1E/subunit epsilon, ATP5PF/subunit F6, ATP5PB/subunit b, ATP5PD/subunit d, ATP5PO/subunit OSCP. ATP synthase complex consists of a soluble F(1) head domain (subunits alpha(3) and beta(3)) - the catalytic core - and a membrane F(0) domain - the membrane proton channel (subunits c, a, 8, e, f, g, k and j). These two domains are linked by a central stalk (subunits gamma, delta, and epsilon) rotating inside the F1 region and a stationary peripheral stalk (subunits F6, b, d, and OSCP).

Subcellular location. Mitochondrion. Mitochondrion inner membrane.

Similarity. Belongs to the ATPase F chain family.

Isoforms (4)

UniProt ID	Names	Canonical?
P56134-1	1	yes
P56134-2	2
P56134-3	3
P56134-4	4

RefSeq proteins (4): NP_001003713, NP_001003714, NP_001034267, NP_004880* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR019344	F1F0-ATPsyn_F_prd	Family

Pfam: PF10206

UniProt features (16 total): helix 5, modified residue 3, splice variant 2, initiator methionine 1, chain 1, strand 1, transmembrane region 1, sequence conflict 1, turn 1

Structure

Experimental structures (PDB)

10 structures.

PDB	Method	Resolution (Å)
8H9S	ELECTRON MICROSCOPY	2.53
8H9U	ELECTRON MICROSCOPY	2.61
8H9F	ELECTRON MICROSCOPY	2.69
8H9T	ELECTRON MICROSCOPY	2.77
8KI3	ELECTRON MICROSCOPY	2.89
8H9M	ELECTRON MICROSCOPY	3
8H9V	ELECTRON MICROSCOPY	3.02
8KHF	ELECTRON MICROSCOPY	3.13
8H9J	ELECTRON MICROSCOPY	3.26
8H9Q	ELECTRON MICROSCOPY	3.47

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-P56134-F1	75.31	0.09

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (3): 2, 3, 22

Function

Pathways and Gene Ontology

Reactome pathways

6 pathways

ID	Pathway
R-HSA-163210	Formation of ATP by chemiosmotic coupling
R-HSA-8949613	Cristae formation
R-HSA-1428517	Aerobic respiration and respiratory electron transport
R-HSA-1430728	Metabolism
R-HSA-1592230	Mitochondrial biogenesis
R-HSA-1852241	Organelle biogenesis and maintenance

MSigDB gene sets: 230 (showing top): MORF_MBD4, MORF_RAB5A, RORA1_01, MODULE_151, STARK_PREFRONTAL_CORTEX_22Q11_DELETION_DN, MORF_RAD21, GOBP_ORGANOPHOSPHATE_METABOLIC_PROCESS, GOBP_NUCLEOSIDE_PHOSPHATE_BIOSYNTHETIC_PROCESS, MORF_PSMC2, GOBP_ORGANOPHOSPHATE_BIOSYNTHETIC_PROCESS, HUMMERICH_SKIN_CANCER_PROGRESSION_DN, REACTOME_FORMATION_OF_ATP_BY_CHEMIOSMOTIC_COUPLING, GOBP_CARBOHYDRATE_DERIVATIVE_METABOLIC_PROCESS, GOBP_MONOATOMIC_CATION_TRANSPORT, PUJANA_CHEK2_PCC_NETWORK

GO Biological Process (5): proton motive force-driven ATP synthesis (GO:0015986), proton motive force-driven mitochondrial ATP synthesis (GO:0042776), proton transmembrane transport (GO:1902600), ATP biosynthetic process (GO:0006754), monoatomic ion transport (GO:0006811)

GO Molecular Function (1): proton-transporting ATP synthase activity, rotational mechanism (GO:0046933)

GO Cellular Component (5): mitochondrion (GO:0005739), mitochondrial inner membrane (GO:0005743), nuclear membrane (GO:0031965), proton-transporting ATP synthase complex (GO:0045259), membrane (GO:0016020)

Reactome top-level categories

Rollup of top-4 pathways:

Category	Pathways
Aerobic respiration and respiratory electron transport	1
Mitochondrial biogenesis	1
Metabolism	1
Organelle biogenesis and maintenance	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
proton motive force-driven ATP synthesis	2
ATP biosynthetic process	1
mitochondrion	1
oxidative phosphorylation	1
monoatomic cation transmembrane transport	1
purine ribonucleotide biosynthetic process	1
purine ribonucleoside triphosphate biosynthetic process	1
ATP metabolic process	1
transport	1
proton channel activity	1
ligase activity	1
cytoplasm	1
intracellular membrane-bounded organelle	1
organelle inner membrane	1
mitochondrial membrane	1
nucleus	1
nuclear envelope	1
organelle membrane	1
proton-transporting two-sector ATPase complex	1
cation channel complex	1
respiratory chain complex	1
catalytic complex	1
cellular anatomical structure	1

Protein interactions and networks

STRING

1798 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
ATP5MF	ATP5PB	P24539	874
ATP5MF	ATP5MG	O75964	869
ATP5MF	ATP5ME	P56385	826
ATP5MF	ATP5PD	O75947	810
ATP5MF	ATP5PF	P18859	810
ATP5MF	ATP5PO	P48047	756
ATP5MF	ATP5F1A	P25705	744
ATP5MF	ATP5F1C	P36542	710
ATP5MF	ATP5F1E	P56381	645
ATP5MF	ATP5F1D	P30049	641
ATP5MF	NDUFA6	P56556	626
ATP5MF	ATP5MC1	P05496	573
ATP5MF	NDUFB7	P17568	544
ATP5MF	COX5B	P10606	541
ATP5MF	ATP6V0E1	O15342	539

IntAct

113 interactions, top by confidence:

A	B	Type	Score
STK24	STRN	psi-mi:“MI:0914”(association)	0.870
ATP5PB	ATP5PD	psi-mi:“MI:0915”(physical association)	0.760
CFTR	ESYT2	psi-mi:“MI:2364”(proximity)	0.710
CFTR	ESYT2	psi-mi:“MI:0914”(association)	0.710
UNC93B1	GPR89A	psi-mi:“MI:0914”(association)	0.530
BLK	EEF1E1	psi-mi:“MI:0914”(association)	0.350
Ncstn	DERL1	psi-mi:“MI:0914”(association)	0.350
ARMC6		psi-mi:“MI:0914”(association)	0.350
NAF1	C1orf226	psi-mi:“MI:0914”(association)	0.350
TUBA1C	TCP11L2	psi-mi:“MI:0914”(association)	0.350
MYC	ILVBL	psi-mi:“MI:0914”(association)	0.350
HA	ESYT2	psi-mi:“MI:0914”(association)	0.350
PB1	HAX1	psi-mi:“MI:0914”(association)	0.350
PB2	ESYT2	psi-mi:“MI:0914”(association)	0.350
NS1	HAX1	psi-mi:“MI:0914”(association)	0.350
	HAX1	psi-mi:“MI:0914”(association)	0.350
M2	ESYT2	psi-mi:“MI:0914”(association)	0.350
MME		psi-mi:“MI:0914”(association)	0.350
COX15	SNRPGP15	psi-mi:“MI:0914”(association)	0.350
SOAT1	SNRPGP15	psi-mi:“MI:0914”(association)	0.350
VDAC1	SNRPGP15	psi-mi:“MI:0914”(association)	0.350
Prdm16	ESYT2	psi-mi:“MI:0914”(association)	0.350

BioGRID (151): ATP5J2 (Affinity Capture-MS), ATP5J2 (Co-fractionation), ATP5J2 (Co-fractionation), ATP5J2 (Co-fractionation), ATP5J2 (Affinity Capture-MS), ATP5J2 (Affinity Capture-MS), ATP5J2 (Affinity Capture-MS), ATP5J2 (Affinity Capture-MS), ATP5J2 (Affinity Capture-MS), ATP5J2 (Affinity Capture-MS), ATP5J2 (Affinity Capture-MS), ATP5J2 (Affinity Capture-MS), ATP5J2 (Affinity Capture-MS), ATP5J2 (Affinity Capture-MS), ATP5J2 (Affinity Capture-MS)

ESM2 similar proteins: A2VDV9, A5PJ82, A8MTT3, D3Z9R8, D3ZAF6, D4ACP2, E7EXZ6, G1QDE8, G1S9B8, O00483, P0DO44, P0DO45, P14790, P46269, P46270, P56134, P56135, P56378, P56379, P56392, P80497, Q01321, Q0MQ97, Q0MQ98, Q0MQ99, Q28851, Q28GF4, Q3SZ44, Q3YAJ5, Q4FZG9, Q4R542, Q5R6T5, Q5RCY6, Q5REX0, Q62425, Q6PBH5, Q78RX3, Q7YRK7, Q8BTC1, Q8BTE5

Diamond homologs: D3ZAF6, P56134, P56135, Q28851, Q5R6T5, Q95339, Q22021, Q9W141

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

12 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	12
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

2038 predictions. Top by Δscore:

Variant	Effect	Δscore
7:99450367:GCACG:G	donor_gain	1.0000
7:99450370:CGGT:C	donor_loss	1.0000
7:99450373:T:G	donor_loss	1.0000
7:99450793:GCA:G	donor_gain	1.0000
7:99450796:G:GG	donor_gain	1.0000
7:99452343:A:AG	acceptor_gain	1.0000
7:99452344:T:G	acceptor_gain	1.0000
7:99452351:T:A	acceptor_gain	1.0000
7:99452352:G:A	acceptor_gain	1.0000
7:99452358:T:A	acceptor_gain	1.0000
7:99454136:GGT:G	donor_loss	1.0000
7:99454137:GTG:G	donor_loss	1.0000
7:99459145:A:AC	donor_gain	1.0000
7:99459146:C:CC	donor_gain	1.0000
7:99460085:CCT:C	donor_gain	1.0000
7:99460191:GTA:G	acceptor_gain	1.0000
7:99460192:TA:T	acceptor_gain	1.0000
7:99460194:C:CC	acceptor_gain	1.0000
7:99460200:C:CT	acceptor_gain	1.0000
7:99450275:GGGGA:G	acceptor_gain	0.9900
7:99450372:G:GG	donor_gain	0.9900
7:99450792:TGCA:T	donor_gain	0.9900
7:99450793:GCAG:G	donor_gain	0.9900
7:99450794:CA:C	donor_gain	0.9900
7:99452355:T:TA	acceptor_gain	0.9900
7:99452471:G:GT	donor_gain	0.9900
7:99453669:G:GG	donor_gain	0.9900
7:99453964:A:AG	acceptor_gain	0.9900
7:99453965:G:GG	acceptor_gain	0.9900
7:99453965:GCAAA:G	acceptor_gain	0.9900

AlphaMissense

610 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
7:99460140:A:G	W29R	0.994
7:99460140:A:T	W29R	0.994
7:99459189:A:G	C72R	0.993
7:99460138:C:A	W29C	0.989
7:99460138:C:G	W29C	0.989
7:99459191:G:T	A71E	0.986
7:99459252:A:C	Y51D	0.986
7:99459209:C:T	G65E	0.985
7:99458354:C:A	K86N	0.984
7:99458354:C:G	K86N	0.984
7:99460086:C:G	G47R	0.984
7:99460148:A:G	L26P	0.984
7:99459221:C:T	G61E	0.983
7:99459222:C:G	G61R	0.983
7:99459222:C:T	G61R	0.983
7:99460139:C:G	W29S	0.983
7:99459240:A:G	Y55H	0.982
7:99458346:C:G	R89P	0.981
7:99458332:G:C	H94D	0.980
7:99460148:A:T	L26Q	0.980
7:99459239:T:G	Y55S	0.979
7:99458340:C:G	R91P	0.978
7:99458336:T:A	K92N	0.977
7:99458336:T:G	K92N	0.977
7:99459179:A:C	L75R	0.977
7:99459197:A:T	V69E	0.976
7:99459210:C:G	G65R	0.975
7:99459210:C:T	G65R	0.975
7:99459252:A:G	Y51H	0.974
7:99459263:C:T	G47D	0.974

dbSNP variants (sampled 300 via entrez): RS1000093689 (7:99467355 C>T), RS1000230096 (7:99461027 A>G), RS1000312676 (7:99466647 G>A,T), RS1000325486 (7:99461377 T>C), RS1000363478 (7:99466911 C>G,T), RS1000603314 (7:99460790 A>C,T), RS1001208341 (7:99462052 G>A), RS1001366203 (7:99468142 C>A,T), RS1001645276 (7:99460373 G>T), RS1001867382 (7:99461772 C>T), RS1001981979 (7:99459053 A>C), RS1002165619 (7:99465162 G>T), RS1002228633 (7:99466441 T>C), RS1002289634 (7:99457836 G>A,T), RS1002489377 (7:99463358 T>C,G)

Disease associations

OMIM: gene MIM:619792 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

7 associations (top):

Study	Trait	p-value
GCST004730_2	Facial emotion recognition (sad faces)	3.000000e-06
GCST005144_1	Tacrolimus trough concentration in kidney transplant patients	2.000000e-17
GCST005335_2	Diffuse cutaneous systemic sclerosis	2.000000e-06
GCST006249_46	Serum metabolite levels	4.000000e-21
GCST007327_6	Smoking status (ever vs never smokers)	3.000000e-10
GCST90002389_160	Lymphocyte percentage of white cells	4.000000e-16
GCST90002399_184	Neutrophil percentage of white cells	2.000000e-15

EFO canonical traits (5, from GWAS)

EFO ID	Trait name
EFO:0008329	facial emotion recognition measurement
EFO:0008458	tacrolimus measurement
EFO:0004318	smoking behavior
EFO:0007993	lymphocyte percentage of leukocytes
EFO:0007990	neutrophil percentage of leukocytes

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067082 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

GtoPdb / IUPHAR curated pharmacology

(IUPHAR/BPS Guide to Pharmacology — expert-curated)

Target class: transporter — F-type ATPase

ChEMBL bioactivities

4 potent at pChembl≥5 of 4 total, top 4 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
8.70	Kd	1.997	nM	CHEMBL5653589
8.70	ED50	1.997	nM	CHEMBL5653589
7.68	Kd	20.65	nM	CHEMBL3752910
7.68	ED50	20.65	nM	CHEMBL3752910

PubChem BioAssay actives

2 with measured affinity, of 4 total; 2 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2147919: Binding affinity to human ATP5J2 incubated for 45 mins by Kinobead based pull down assay	kd	0.0020	uM
4-methyl-3-[(1-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2147919: Binding affinity to human ATP5J2 incubated for 45 mins by Kinobead based pull down assay	kd	0.0207	uM

CTD chemical–gene interactions

51 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	increases expression, affects expression, affects cotreatment, decreases methylation, decreases expression	4
sodium arsenite	decreases expression, increases abundance, increases expression	4
Acetaminophen	affects cotreatment, decreases expression	2
Valproic Acid	decreases expression, increases methylation	2
Cyclosporine	decreases expression	2
Particulate Matter	decreases expression, increases abundance	2
aristolochic acid I	increases expression	1
bisphenol F	affects cotreatment, increases expression	1
bufotalin	increases expression	1
triphenyl phosphate	affects expression	1
glycidyl methacrylate	decreases expression	1
O,O-diethyl O-3,5,6-trichloro-2-pyridyl phosphate	affects expression, affects response to substance	1
beta-lapachone	decreases expression	1
tris(1,3-dichloro-2-propyl)phosphate	decreases expression	1
cobaltous chloride	decreases expression	1
chloropicrin	increases expression	1
K 7174	decreases expression	1
ICG 001	decreases expression	1
bisphenol S	affects cotreatment, increases expression	1
Fulvestrant	affects cotreatment, decreases methylation	1
Air Pollutants	decreases expression, increases abundance	1
Arsenic	increases abundance, increases expression	1
Atrazine	decreases expression	1
Caffeine	decreases expression	1
Dexamethasone	affects cotreatment, increases expression	1
Diethylstilbestrol	decreases expression	1
Dimethyl Sulfoxide	affects expression	1
Diuron	decreases expression	1
Doxorubicin	increases expression	1
Estradiol	decreases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5650961	Binding	Binding affinity to human ATP5J2 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Cellosaurus cell lines

1 cell lines: 1 transformed cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B2SH	Abcam HEK293T ATP5MF KO	Transformed cell line	Female

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.