ATP5PD

Summary

ATP5PD (ATP synthase peripheral stalk subunit d, HGNC:845) is a protein-coding gene on chromosome 17q25.1, encoding ATP synthase peripheral stalk subunit d, mitochondrial (O75947). Subunit d, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is a selective cancer dependency (DepMap: 21.9% of cell lines).

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15.

Source: NCBI Gene 10476 — RefSeq curated summary.

At a glance

• GWAS associations: 2
• Clinical variants (ClinVar): 29 total
• Druggable target: yes
• Cancer dependency (DepMap): dependent in 21.9% of screened cell lines
• MANE Select transcript: NM_006356

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:845
Approved symbol	ATP5PD
Name	ATP synthase peripheral stalk subunit d
Location	17q25.1
Locus type	gene with protein product
Status	Approved
Aliases	ATPQ, ATP5JD
Ensembl gene	ENSG00000167863
Ensembl biotype	protein_coding
OMIM	618121
Entrez	10476

Gene structure

Transcript identifiers

Ensembl transcripts: 20 — 17 protein_coding, 2 retained_intron, 1 nonsense_mediated_decay

ENST00000301587, ENST00000344546, ENST00000538432, ENST00000580649, ENST00000582341, ENST00000909788, ENST00000909789, ENST00000918881, ENST00000918882, ENST00000918883, ENST00000918884, ENST00000918885, ENST00000918886, ENST00000918887, ENST00000918888, ENST00000918889, ENST00000918890, ENST00000918891, ENST00000918892, ENST00000918893

RefSeq mRNA: 2 — MANE Select: NM_006356 NM_001003785, NM_006356

CCDS: CCDS11712, CCDS32727

Canonical transcript exons

ENST00000301587 — 6 exons

Exon	Start	End
ENSE00001117770	75042181	75042277
ENSE00001117773	75040092	75040163
ENSE00001389954	75042529	75042659
ENSE00002695382	75046925	75046969
ENSE00003476451	75038863	75039063
ENSE00003599114	75039209	75039271

Expression profiles

Bgee: expression breadth ubiquitous, 298 present calls, max score 99.72.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 235.1540 / max 6581.4593, expressed in 1828 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
168031	235.1540	1828

Top tissues by expression

298 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
heart right ventricle	UBERON:0002080	99.72	gold quality
myocardium	UBERON:0002349	99.65	gold quality
left ventricle myocardium	UBERON:0006566	99.58	gold quality
cardiac atrium	UBERON:0002081	99.57	gold quality
cardiac ventricle	UBERON:0002082	99.57	gold quality
heart left ventricle	UBERON:0002084	99.57	gold quality
skeletal muscle tissue of biceps brachii	UBERON:0004502	99.57	gold quality
right atrium auricular region	UBERON:0006631	99.57	gold quality
biceps brachii	UBERON:0001507	99.56	gold quality
cardiac muscle of right atrium	UBERON:0003379	99.55	gold quality
apex of heart	UBERON:0002098	99.54	gold quality
body of tongue	UBERON:0011876	99.53	gold quality
heart	UBERON:0000948	99.51	gold quality
skeletal muscle tissue of rectus abdominis	UBERON:0004511	99.47	gold quality
gastrocnemius	UBERON:0001388	99.44	gold quality
hindlimb stylopod muscle	UBERON:0004252	99.44	gold quality
upper leg skin	UBERON:0004262	99.44	gold quality
pharyngeal mucosa	UBERON:0000355	99.43	gold quality
muscle of leg	UBERON:0001383	99.43	gold quality
tibialis anterior	UBERON:0001385	99.43	gold quality
triceps brachii	UBERON:0001509	99.41	gold quality
muscle organ	UBERON:0001630	99.39	gold quality
gluteal muscle	UBERON:0002000	99.39	gold quality
vena cava	UBERON:0004087	99.39	gold quality
skeletal muscle organ	UBERON:0014892	99.39	gold quality
mucosa of sigmoid colon	UBERON:0004993	99.37	gold quality
mammalian vulva	UBERON:0000997	99.35	gold quality
diaphragm	UBERON:0001103	99.35	gold quality
tongue	UBERON:0001723	99.35	gold quality
oral cavity	UBERON:0000167	99.34	gold quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-MTAB-9388	no	1328.44
E-HCAD-31	no	1.88
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): RBPJ

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 21.9% of screened cell lines.

Literature-anchored findings (GeneRIF, showing 5)

• This protein has been found differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia. (PMID:19110265)
• By merging results of a meta-GWAS, results in the CHARGE consortium data sets and an in vivo genotyping comprising 4501 individuals, detected a novel locus ATP5H/KCTD2 associated with Alzheimer’s disease risk (PMID:23857120)
• The Network analyses identified ATP5H expression in temporal cortex in patient with late-onset Alzheimer’s disease. (PMID:28242297)
• ATP5H loss in the tumor is strongly linked to failure of therapy, disease progression, and poor survival in patients with cancer. (PMID:30124467)
• Meta-analysis of brain samples of individuals with schizophrenia detects down-regulation of multiple ATP synthase encoding genes in both females and males. (PMID:36640659)

Cross-species orthologs

6 orthologs

Organism	Symbol	Gene ID
danio_rerio	atp5pd	ENSDARG00000098355
mus_musculus	Atp5pd	ENSMUSG00000034566
rattus_norvegicus	Atp5pd	ENSRNOG00000003626
rattus_norvegicus		ENSRNOG00000030158
drosophila_melanogaster	knon	FBGN0285943
caenorhabditis_elegans		WBGENE00007385

Protein

Protein identifiers

ATP synthase peripheral stalk subunit d, mitochondrial — O75947 (reviewed: O75947)

Alternative names: ATP synthase peripheral stalk subunit d

All UniProt accessions (2): O75947, F5H608

UniProt curated annotations — full annotation on UniProt →

Function. Subunit d, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. Part of the complex F(0) domain. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.

Subunit / interactions. Component of the ATP synthase complex composed at least of ATP5F1A/subunit alpha, ATP5F1B/subunit beta, ATP5MC1/subunit c (homooctamer), MT-ATP6/subunit a, MT-ATP8/subunit 8, ATP5ME/subunit e, ATP5MF/subunit f, ATP5MG/subunit g, ATP5MK/subunit k, ATP5MJ/subunit j, ATP5F1C/subunit gamma, ATP5F1D/subunit delta, ATP5F1E/subunit epsilon, ATP5PF/subunit F6, ATP5PB/subunit b, ATP5PD/subunit d, ATP5PO/subunit OSCP. ATP synthase complex consists of a soluble F(1) head domain (subunits alpha(3) and beta(3)) - the catalytic core - and a membrane F(0) domain - the membrane proton channel (subunits c, a, 8, e, f, g, k and j). These two domains are linked by a central stalk (subunits gamma, delta, and epsilon) rotating inside the F1 region and a stationary peripheral stalk (subunits F6, b, d, and OSCP). Interacts with FLVCR2; this interaction occurs in the absence of heme and is disrupted upon heme binding.

Subcellular location. Mitochondrion. Mitochondrion inner membrane.

Similarity. Belongs to the ATPase d subunit family.

Isoforms (2)

UniProt ID	Names	Canonical?
O75947-1	1	yes
O75947-2	2

RefSeq proteins (2): NP_001003785, NP_006347* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR008689	ATP_synth_F0_dsu_mt	Family
IPR036228	ATP_synth_F0_dsu_sf_mt	Homologous_superfamily

Pfam: PF05873

UniProt features (30 total): modified residue 15, helix 9, turn 2, initiator methionine 1, chain 1, splice variant 1, strand 1

Structure

Experimental structures (PDB)

14 structures.

PDB	Method	Resolution (Å)
8H9S	ELECTRON MICROSCOPY	2.53
8H9U	ELECTRON MICROSCOPY	2.61
8H9F	ELECTRON MICROSCOPY	2.69
8H9T	ELECTRON MICROSCOPY	2.77
8KI3	ELECTRON MICROSCOPY	2.89
8H9G	ELECTRON MICROSCOPY	2.95
8H9M	ELECTRON MICROSCOPY	3
8H9V	ELECTRON MICROSCOPY	3.02
8KHF	ELECTRON MICROSCOPY	3.13
8H9J	ELECTRON MICROSCOPY	3.26
8H9Q	ELECTRON MICROSCOPY	3.47
8H9K	ELECTRON MICROSCOPY	3.51
8H9N	ELECTRON MICROSCOPY	3.56
8H9R	ELECTRON MICROSCOPY	3.97

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-O75947-F1	87.11	0.45

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (15): 95, 95, 117, 144, 144, 149, 149, 2, 32, 63, 72, 78, 78, 85, 85

Function

Pathways and Gene Ontology

Reactome pathways

8 pathways

ID	Pathway
R-HSA-163210	Formation of ATP by chemiosmotic coupling
R-HSA-8949613	Cristae formation
R-HSA-9837999	Mitochondrial protein degradation
R-HSA-1428517	Aerobic respiration and respiratory electron transport
R-HSA-1430728	Metabolism
R-HSA-1592230	Mitochondrial biogenesis
R-HSA-1852241	Organelle biogenesis and maintenance
R-HSA-392499	Metabolism of proteins

MSigDB gene sets: 175 (showing top): STARK_PREFRONTAL_CORTEX_22Q11_DELETION_DN, GOBP_ORGANOPHOSPHATE_METABOLIC_PROCESS, GOBP_NUCLEOSIDE_PHOSPHATE_BIOSYNTHETIC_PROCESS, CAGCTG_AP4_Q5, GOBP_ORGANOPHOSPHATE_BIOSYNTHETIC_PROCESS, YY1_Q6, REACTOME_FORMATION_OF_ATP_BY_CHEMIOSMOTIC_COUPLING, GOBP_CARBOHYDRATE_DERIVATIVE_METABOLIC_PROCESS, GOBP_MONOATOMIC_CATION_TRANSPORT, GOBP_NUCLEOBASE_CONTAINING_SMALL_MOLECULE_METABOLIC_PROCESS, GOBP_GENERATION_OF_PRECURSOR_METABOLITES_AND_ENERGY, MORF_SKP1A, MARTINEZ_RB1_TARGETS_DN, GOBP_ATP_BIOSYNTHETIC_PROCESS, GOBP_OXIDATIVE_PHOSPHORYLATION

GO Biological Process (4): proton motive force-driven ATP synthesis (GO:0015986), proton motive force-driven mitochondrial ATP synthesis (GO:0042776), monoatomic ion transport (GO:0006811), proton transmembrane transport (GO:1902600)

GO Molecular Function (3): proton transmembrane transporter activity (GO:0015078), protein binding (GO:0005515), proton-transporting ATP synthase activity, rotational mechanism (GO:0046933)

GO Cellular Component (4): mitochondrion (GO:0005739), mitochondrial inner membrane (GO:0005743), proton-transporting ATP synthase complex (GO:0045259), membrane (GO:0016020)

Reactome top-level categories

Rollup of top-5 pathways:

Category	Pathways
Aerobic respiration and respiratory electron transport	1
Mitochondrial biogenesis	1
Metabolism of proteins	1
Metabolism	1
Organelle biogenesis and maintenance	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
proton motive force-driven ATP synthesis	2
ATP biosynthetic process	1
mitochondrion	1
oxidative phosphorylation	1
transport	1
monoatomic cation transmembrane transport	1
monoatomic cation transmembrane transporter activity	1
proton transmembrane transport	1
binding	1
proton channel activity	1
ligase activity	1
cytoplasm	1
intracellular membrane-bounded organelle	1
organelle inner membrane	1
mitochondrial membrane	1
proton-transporting two-sector ATPase complex	1
cation channel complex	1
respiratory chain complex	1
catalytic complex	1
cellular anatomical structure	1

Protein interactions and networks

STRING

2737 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
ATP5PD	ATP5PO	P48047	907
ATP5PD	ATP5PF	P18859	874
ATP5PD	ATP5PB	P24539	864
ATP5PD	ATP5F1D	P30049	847
ATP5PD	SLC25A19	Q9HC21	845
ATP5PD	ATP5F1B	P06576	838
ATP5PD	ATP5F1A	P25705	832
ATP5PD	ATP5MG	O75964	820
ATP5PD	ATP5F1C	P36542	816
ATP5PD	ATP5MF	P56134	810
ATP5PD	ATP5F1E	P56381	793
ATP5PD	ATP5ME	P56385	791
ATP5PD	ATP5MC3	P48201	783
ATP5PD	COX5A	P20674	775
ATP5PD	KCTD2	Q14681	701

IntAct

188 interactions, top by confidence:

A	B	Type	Score
MED4	MED19	psi-mi:“MI:0914”(association)	0.900
ATP5PD	ATP5PB	psi-mi:“MI:0915”(physical association)	0.760
ATP5PB	ATP5PD	psi-mi:“MI:0915”(physical association)	0.760
NDUFS3	NDUFS8	psi-mi:“MI:0914”(association)	0.730
PRELID3B	TRIAP1	psi-mi:“MI:0914”(association)	0.710
CFTR	ESYT2	psi-mi:“MI:0914”(association)	0.710
ATP5PD	ATP5PF	psi-mi:“MI:0915”(physical association)	0.670
ATP5F1B	ATP5PD	psi-mi:“MI:0915”(physical association)	0.670
ATP5F1B	ATP5PD	psi-mi:“MI:0914”(association)	0.670
ATP5PF	ATP5PD	psi-mi:“MI:0914”(association)	0.670
ATP5PB	SLC19A2	psi-mi:“MI:0914”(association)	0.640
CFTR	HAX1	psi-mi:“MI:0914”(association)	0.610
STK4	STRN	psi-mi:“MI:0914”(association)	0.610
CANX	PGRMC1	psi-mi:“MI:0914”(association)	0.570
ATP5F1D	NDUFB5	psi-mi:“MI:0914”(association)	0.530
ATP5ME	SLC19A2	psi-mi:“MI:0914”(association)	0.530
ATP5PF	SLC19A2	psi-mi:“MI:0914”(association)	0.530
ATP5F1B	SCAMP2	psi-mi:“MI:0914”(association)	0.530
UNC93B1	GPR89A	psi-mi:“MI:0914”(association)	0.530
STOM	EI24	psi-mi:“MI:0914”(association)	0.510
vpu	SCAMP3	psi-mi:“MI:0914”(association)	0.460
TNFAIP3	LRRIQ3	psi-mi:“MI:0914”(association)	0.420
BRD1	ATP5PD	psi-mi:“MI:0915”(physical association)	0.400

BioGRID (350): ATP5H (Affinity Capture-MS), ATP5H (Affinity Capture-MS), ATP5H (Affinity Capture-MS), ATP5D (Co-fractionation), ATP5F1 (Co-fractionation), ATP5H (Co-fractionation), ATP5H (Co-fractionation), ATP5H (Co-fractionation), ATP5H (Co-fractionation), ATP5H (Co-fractionation), ATP5H (Co-fractionation), ATP5I (Co-fractionation), ATP5O (Co-fractionation), CHCHD2 (Co-fractionation), CISD1 (Co-fractionation)

ESM2 similar proteins: A1CXH2, A2QI68, A3LNG8, A3LQD9, A5D9S8, A5DM93, A5DSD2, A5DTG8, A5DY61, B0Y5Z6, B2WBQ6, B6QHK6, B8MJK3, B9WLF1, C0HK60, C4YLH0, C5E325, C5M3V6, C7YIH6, O13350, O42911, O75947, O94373, O94390, P05626, P0C2C8, P13620, P30902, P31399, P32453, P34748, P40513, P42116, P87127, Q02204, Q0V4H8, Q19126, Q20053, Q22505, Q24251

Diamond homologs: O75947, P13620, P31399, Q24251, Q9DCX2, P0C2C8

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
ATP5PD	“form complex”	“ATP synthase”	binding

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 200 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Formation of ATP by chemiosmotic coupling	7	30.3×	4e-07
Cristae formation	7	18.4×	8e-06
Mitochondrial protein import	11	14.0×	1e-07
Mitochondrial biogenesis	8	10.2×	9e-05
Mitochondrial protein degradation	11	9.5×	2e-06
Aerobic respiration and respiratory electron transport	13	8.7×	5e-07
SLC-mediated transmembrane transport	11	4.9×	1e-03

GO biological processes:

GO term	Partners	Fold	FDR
proton motive force-driven ATP synthesis	7	32.5×	6e-07
proton motive force-driven mitochondrial ATP synthesis	12	18.3×	2e-09

Disease & clinical

Clinical variants and AI predictions

ClinVar

29 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	23
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

832 predictions. Top by Δscore:

Variant	Effect	Δscore
17:75039061:CAT:C	acceptor_gain	1.0000
17:75039064:C:CC	acceptor_gain	1.0000
17:75039064:C:T	acceptor_loss	1.0000
17:75039065:T:A	acceptor_loss	1.0000
17:75039671:C:CT	donor_gain	1.0000
17:75039672:T:TT	donor_gain	1.0000
17:75040090:A:AC	donor_gain	1.0000
17:75040091:C:CC	donor_gain	1.0000
17:75040091:CAT:C	donor_gain	1.0000
17:75040164:C:CC	acceptor_gain	1.0000
17:75042178:CA:C	donor_loss	1.0000
17:75042179:A:AC	donor_gain	1.0000
17:75042179:A:C	donor_loss	1.0000
17:75042180:C:CC	donor_gain	1.0000
17:75042180:CCTT:C	donor_gain	1.0000
17:75042273:CCAAC:C	acceptor_gain	1.0000
17:75042274:CAAC:C	acceptor_gain	1.0000
17:75042274:CAACC:C	acceptor_gain	1.0000
17:75042275:AAC:A	acceptor_gain	1.0000
17:75042276:AC:A	acceptor_gain	1.0000
17:75042277:CC:C	acceptor_gain	1.0000
17:75042278:C:CC	acceptor_gain	1.0000
17:75042278:CTG:C	acceptor_loss	1.0000
17:75042279:T:G	acceptor_loss	1.0000
17:75042283:C:CT	acceptor_gain	1.0000
17:75042283:C:T	acceptor_gain	1.0000
17:75042285:C:CT	acceptor_gain	1.0000
17:75042286:A:T	acceptor_gain	1.0000
17:75039060:CCAT:C	acceptor_gain	0.9900
17:75039061:CATC:C	acceptor_gain	0.9900

AlphaMissense

1067 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
17:75038961:A:G	W153R	0.984
17:75038961:A:T	W153R	0.984
17:75038959:C:A	W153C	0.977
17:75038959:C:G	W153C	0.977
17:75038998:G:C	F140L	0.974
17:75038998:G:T	F140L	0.974
17:75039000:A:G	F140L	0.974
17:75042238:C:A	W54C	0.971
17:75042238:C:G	W54C	0.971
17:75042240:A:G	W54R	0.971
17:75042240:A:T	W54R	0.971
17:75042612:C:A	W13C	0.961
17:75042612:C:G	W13C	0.961
17:75042614:A:G	W13R	0.958
17:75042614:A:T	W13R	0.958
17:75039029:T:G	Q130P	0.957
17:75042559:A:G	L31P	0.954
17:75042239:C:G	W54S	0.953
17:75040161:A:C	F74L	0.952
17:75040161:A:T	F74L	0.952
17:75040163:A:G	F74L	0.952
17:75038958:G:A	P154S	0.942
17:75039025:C:A	M131I	0.942
17:75039025:C:G	M131I	0.942
17:75039025:C:T	M131I	0.942
17:75038955:G:C	H155D	0.940
17:75038960:C:G	W153S	0.935
17:75042245:A:T	I52N	0.933
17:75042538:A:G	L38P	0.933
17:75042555:T:A	K32N	0.932

dbSNP variants (sampled 300 via entrez): RS1000204754 (17:75043094 C>T), RS1000486566 (17:75042836 G>C), RS1000537240 (17:75044945 G>T), RS1001093033 (17:75040817 C>T), RS1001435267 (17:75038567 G>A), RS1001616314 (17:75042287 A>C,G), RS1001897919 (17:75043625 A>C,T), RS1001924246 (17:75040576 T>C), RS1001958847 (17:75048652 C>T), RS1002433252 (17:75046519 C>A,G,T), RS1003031027 (17:75045372 T>G), RS1003341394 (17:75042718 A>G), RS1003443607 (17:75041204 C>T), RS1003535688 (17:75048699 G>T), RS1003901145 (17:75046483 C>A,T)

Disease associations

OMIM: gene MIM:618121 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

2 associations (top):

Study	Trait	p-value
GCST003423_1	Alzheimer’s disease or small vessel stroke	2.000000e-08
GCST008103_113	Bipolar disorder	4.000000e-06

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:1001504	small vessel stroke

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6066999 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

GtoPdb / IUPHAR curated pharmacology

(IUPHAR/BPS Guide to Pharmacology — expert-curated)

Target class: transporter — F-type ATPase

ChEMBL bioactivities

4 potent at pChembl≥5 of 4 total, top 4 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
7.25	Kd	55.73	nM	CHEMBL5653589
7.25	ED50	55.73	nM	CHEMBL5653589
7.14	Kd	72.67	nM	CHEMBL3752910
7.14	ED50	72.67	nM	CHEMBL3752910

PubChem BioAssay actives

2 with measured affinity, of 4 total; 2 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2147917: Binding affinity to human ATP5H incubated for 45 mins by Kinobead based pull down assay	kd	0.0557	uM
4-methyl-3-[(1-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2147917: Binding affinity to human ATP5H incubated for 45 mins by Kinobead based pull down assay	kd	0.0727	uM

CTD chemical–gene interactions

47 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	affects expression, increases expression	3
Doxorubicin	affects expression, decreases expression	2
dicrotophos	decreases expression	1
triphenyl phosphate	affects expression	1
deoxynivalenol	decreases expression	1
O,O-diethyl O-3,5,6-trichloro-2-pyridyl phosphate	affects expression, affects response to substance	1
arsenite	affects binding, increases reaction	1
sodium arsenite	decreases expression, increases abundance	1
3-methyladenine	affects cotreatment, decreases expression, decreases reaction	1
benzo(e)pyrene	increases methylation	1
1-nitropyrene	decreases expression, affects cotreatment, decreases reaction	1
diallyl trisulfide	decreases expression, increases expression	1
microcystin RR	decreases expression	1
perfluorooctane sulfonic acid	decreases expression	1
chloropicrin	increases expression	1
corosolic acid	decreases expression	1
bisphenol S	increases expression	1
bisphenol AF	increases expression	1
Ethanol	affects cotreatment, decreases expression, increases abundance	1
Arsenic	increases abundance, decreases expression	1
Atrazine	decreases expression	1
Vehicle Emissions	decreases expression	1
Benzo(a)pyrene	affects methylation	1
Diuron	decreases expression	1
Dopamine	increases expression	1
Drugs, Chinese Herbal	decreases expression	1
Estradiol	decreases expression	1
Gasoline	affects cotreatment, decreases expression, increases abundance	1
Hydrogen Peroxide	decreases expression	1
Isoniazid	increases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5650959	Binding	Binding affinity to human ATP5H incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.