AUNIP

Summary

AUNIP (aurora kinase A and ninein interacting protein, HGNC:28363) is a protein-coding gene on chromosome 1p36.11, encoding Aurora kinase A- and ninein-interacting protein (Q9H7T9). DNA-binding protein that accumulates at DNA double-strand breaks (DSBs) following DNA damage and promotes DNA resection and homologous recombination.

Enables damaged DNA binding activity. Involved in double-strand break repair via homologous recombination; negative regulation of double-strand break repair via nonhomologous end joining; and spindle organization. Located in centrosome; site of DNA damage; and spindle pole.

Source: NCBI Gene 79000 — RefSeq curated summary.

At a glance

• GWAS associations: 2
• Clinical variants (ClinVar): 62 total
• MANE Select transcript: NM_024037

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:28363
Approved symbol	AUNIP
Name	aurora kinase A and ninein interacting protein
Location	1p36.11
Locus type	gene with protein product
Status	Approved
Aliases	MGC2603, AIBp
Ensembl gene	ENSG00000127423
Ensembl biotype	protein_coding
OMIM	620397
Entrez	79000

Gene structure

Transcript identifiers

Ensembl transcripts: 3 — 2 protein_coding, 1 protein_coding_CDS_not_defined

ENST00000374298, ENST00000481602, ENST00000538789

RefSeq mRNA: 2 — MANE Select: NM_024037 NM_001287490, NM_024037

CCDS: CCDS266, CCDS72731

Canonical transcript exons

ENST00000374298 — 3 exons

Exon	Start	End
ENSE00000872558	25837413	25837554
ENSE00001155512	25834006	25835846
ENSE00001463075	25859280	25859457

Expression profiles

Bgee: expression breadth ubiquitous, 170 present calls, max score 97.62.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 8.7287 / max 107.7758, expressed in 1220 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
11097	8.7287	1220

Top tissues by expression

280 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
oocyte	CL:0000023	97.62	gold quality
secondary oocyte	CL:0000655	96.37	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	91.10	gold quality
buccal mucosa cell	CL:0002336	86.57	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	84.00	gold quality
sperm	CL:0000019	80.91	gold quality
right testis	UBERON:0004534	80.60	gold quality
testis	UBERON:0000473	79.94	gold quality
left testis	UBERON:0004533	79.92	gold quality
male germ cell	CL:0000015	79.34	silver quality
ventricular zone	UBERON:0003053	73.62	gold quality
ganglionic eminence	UBERON:0004023	71.58	gold quality
bone marrow	UBERON:0002371	71.43	gold quality
embryo	UBERON:0000922	71.09	gold quality
bone marrow cell	CL:0002092	70.28	silver quality
stromal cell of endometrium	CL:0002255	66.75	gold quality
prefrontal cortex	UBERON:0000451	66.22	gold quality
trabecular bone tissue	UBERON:0002483	65.37	silver quality
placenta	UBERON:0001987	64.87	gold quality
pancreatic ductal cell	CL:0002079	64.23	silver quality
right adrenal gland cortex	UBERON:0035827	64.03	gold quality
cortical plate	UBERON:0005343	63.87	gold quality
mucosa of transverse colon	UBERON:0004991	63.59	gold quality
right adrenal gland	UBERON:0001233	63.36	gold quality
left adrenal gland	UBERON:0001234	62.40	gold quality
lower esophagus mucosa	UBERON:0035834	62.22	gold quality
esophagus mucosa	UBERON:0002469	61.57	gold quality
primary visual cortex	UBERON:0002436	61.56	gold quality
left adrenal gland cortex	UBERON:0035825	61.45	gold quality
monocyte	CL:0000576	61.12	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	3.61

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

56 targeting AUNIP, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3613-3P	100.00	76.36	7965
HSA-MIR-30A-5P	100.00	76.31	3233
HSA-MIR-30B-5P	100.00	76.29	3248
HSA-MIR-30C-5P	100.00	76.29	3248
HSA-MIR-30D-5P	100.00	76.32	3233
HSA-MIR-30E-5P	100.00	76.32	3242
HSA-MIR-4668-3P	100.00	68.74	2635
HSA-MIR-371B-5P	99.99	75.34	4759
HSA-MIR-6759-5P	99.99	66.54	785
HSA-MIR-373-5P	99.98	75.36	4753
HSA-MIR-616-5P	99.98	75.58	4775
HSA-MIR-520D-5P	99.98	73.34	4883
HSA-MIR-524-5P	99.98	73.43	4882
HSA-MIR-6793-5P	99.97	65.95	758
HSA-MIR-6780A-5P	99.88	66.69	2776
HSA-MIR-4496	99.88	68.89	2236
HSA-MIR-8073	99.86	65.21	1118
HSA-MIR-383-3P	99.85	65.84	1359
HSA-MIR-4420	99.82	70.08	1624
HSA-MIR-181B-2-3P	99.81	70.06	1646
HSA-MIR-181B-3P	99.81	70.06	1646
HSA-MIR-3934-3P	99.76	65.51	1351
HSA-MIR-7856-5P	99.75	69.99	2901
HSA-MIR-4729	99.69	72.18	4233
HSA-MIR-3202	99.66	67.70	2737
HSA-MIR-3175	99.65	66.30	2031
HSA-MIR-3685	99.62	68.83	1621
HSA-MIR-1260A	99.61	66.67	1098
HSA-MIR-1260B	99.61	66.67	1098
HSA-MIR-5004-3P	99.54	68.27	1371

Literature-anchored findings (GeneRIF, showing 2)

• AIBp may not only be required for the dynamic movement of Aurora-A at the centrosomes and spindle apparatus during the cell cycle, but may also be important during brain tumorigenesis. (PMID:20596670)
• AUNIP could serve as a candidate diagnostic and prognostic biomarker for OSCC and suppression of AUNIP may be a potential approach to preventing and treating OSCC. (PMID:31409573)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Aunip	ENSMUSG00000078521
rattus_norvegicus	Aunip	ENSRNOG00000022030

Protein

Protein identifiers

Aurora kinase A- and ninein-interacting protein — Q9H7T9 (reviewed: Q9H7T9)

All UniProt accessions (1): Q9H7T9

UniProt curated annotations — full annotation on UniProt →

Function. DNA-binding protein that accumulates at DNA double-strand breaks (DSBs) following DNA damage and promotes DNA resection and homologous recombination. Serves as a sensor of DNA damage: binds DNA with a strong preference for DNA substrates that mimic structures generated at stalled replication forks, and anchors RBBP8/CtIP to DSB sites to promote DNA end resection and ensuing homologous recombination repair. Inhibits non-homologous end joining (NHEJ). Required for the dynamic movement of AURKA at the centrosomes and spindle apparatus during the cell cycle.

Subunit / interactions. Interacts (via C-terminus) with AURKA (via C-terminus). Interacts (via N-terminus) with NIN; this interaction blocks NIN phosphorylation by both AURKA and GSK3B. Identified in a complex with NIN and AURKA. Interacts with RBBP8/CtIP.

Subcellular location. Nucleus. Chromosome. Cytoplasm. Cytoskeleton. Microtubule organizing center. Centrosome. Spindle pole.

Tissue specificity. Expressed in heart, skeletal muscles, placenta and testis.

Induction. Overexpressed in brain tumors.

Similarity. Belongs to the AUNIP family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q9H7T9-1	1	yes
Q9H7T9-2	2

RefSeq proteins (2): NP_001274419, NP_076942* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR029286	AUNIP	Family

Pfam: PF15334

UniProt features (9 total): region of interest 3, modified residue 2, chain 1, compositionally biased region 1, splice variant 1, sequence variant 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9H7T9-F1	51.65	0.02

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (2): 267, 292

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 116 (showing top): GOBP_REGULATION_OF_DOUBLE_STRAND_BREAK_REPAIR, ELVIDGE_HYPOXIA_DN, GOBP_NEGATIVE_REGULATION_OF_DNA_REPAIR, GOBP_REGULATION_OF_DNA_REPAIR, GOCC_MICROTUBULE_ORGANIZING_CENTER, GOCC_CENTROSOME, GOBP_REGULATION_OF_RESPONSE_TO_STRESS, GOBP_DNA_DAMAGE_RESPONSE, WEST_ADRENOCORTICAL_CARCINOMA_VS_ADENOMA_UP, GOBP_REGULATION_OF_CELLULAR_RESPONSE_TO_STRESS, FISCHER_DREAM_TARGETS, GOBP_RECOMBINATIONAL_REPAIR, GOCC_SPINDLE, GOBP_CELL_CYCLE_PROCESS, GOBP_DNA_METABOLIC_PROCESS

GO Biological Process (5): double-strand break repair via homologous recombination (GO:0000724), spindle organization (GO:0007051), negative regulation of double-strand break repair via nonhomologous end joining (GO:2001033), DNA repair (GO:0006281), DNA damage response (GO:0006974)

GO Molecular Function (3): damaged DNA binding (GO:0003684), DNA binding (GO:0003677), protein binding (GO:0005515)

GO Cellular Component (7): spindle pole (GO:0000922), nucleus (GO:0005634), centrosome (GO:0005813), site of DNA damage (GO:0090734), chromosome (GO:0005694), cytoplasm (GO:0005737), cytoskeleton (GO:0005856)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	3
intracellular membraneless organelle	2
recombinational repair	1
double-strand break repair	1
microtubule cytoskeleton organization	1
cell cycle process	1
double-strand break repair via nonhomologous end joining	1
negative regulation of double-strand break repair	1
regulation of double-strand break repair via nonhomologous end joining	1
DNA metabolic process	1
DNA damage response	1
cellular response to stress	1
DNA binding	1
nucleic acid binding	1
binding	1
spindle	1
intracellular membrane-bounded organelle	1
centriole	1
microtubule organizing center	1
chromosome	1
intracellular anatomical structure	1

Protein interactions and networks

STRING

702 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
AUNIP	DTL	Q9NZJ0	623
AUNIP	LRRC74B	Q6ZQY2	479
AUNIP	ERCC6L	Q2NKX8	477
AUNIP	A0A087WUM3	A0A087WUM3	446
AUNIP	UHRF1	Q96T88	441
AUNIP	ANLN	Q9NQW6	395
AUNIP	AIRIM	Q9NX04	369
AUNIP	C6orf136	Q5SQH8	349
AUNIP	TAX1BP1	Q86VP1	341
AUNIP	ECT2	Q9H8V3	340
AUNIP	KDM8	Q8N371	329
AUNIP	ZFP69	Q49AA0	324
AUNIP	RPA4	Q13156	316
AUNIP	ZNF593	O00488	311
AUNIP	TENT5B	Q96A09	306

IntAct

40 interactions, top by confidence:

A	B	Type	Score
AUNIP	TRIM68	psi-mi:“MI:0915”(physical association)	0.560
C14orf119	AUNIP	psi-mi:“MI:0915”(physical association)	0.560
AUNIP	NXF1	psi-mi:“MI:0915”(physical association)	0.560
PRKAB2	AUNIP	psi-mi:“MI:0915”(physical association)	0.560
AUNIP	C14orf119	psi-mi:“MI:0915”(physical association)	0.560
AUNIP	DR1	psi-mi:“MI:0915”(physical association)	0.560
AUNIP		psi-mi:“MI:0915”(physical association)	0.560
AUNIP	FGFR3	psi-mi:“MI:0915”(physical association)	0.560
AUNIP	GSN	psi-mi:“MI:0915”(physical association)	0.560
AUNIP	HRAS	psi-mi:“MI:0915”(physical association)	0.560
AUNIP	GTF3C3	psi-mi:“MI:0915”(physical association)	0.560
AUNIP	TRIM68	psi-mi:“MI:0914”(association)	0.560
AURKA	WDR62	psi-mi:“MI:0914”(association)	0.530
GRB2	AUNIP	psi-mi:“MI:0915”(physical association)	0.490

BioGRID (25): AUNIP (Affinity Capture-MS), AUNIP (Two-hybrid), AUNIP (Two-hybrid), AUNIP (Affinity Capture-MS), TRIM68 (Affinity Capture-MS), AUNIP (Two-hybrid), AUNIP (Reconstituted Complex), AUNIP (Affinity Capture-Western), AUNIP (Reconstituted Complex), AUNIP (Biochemical Activity), PRKAB2 (Two-hybrid), NXF1 (Two-hybrid), C14orf119 (Two-hybrid), AUNIP (Affinity Capture-MS), AUNIP (Affinity Capture-MS)

ESM2 similar proteins: A0JM80, A0JNH1, A0JNH9, A3KMW7, A6NMK8, A6QNQ6, B0BM16, B1H1S4, B1WC58, B2GUZ2, D3Z987, D3ZF42, E7FAP1, F6SNN2, P23497, P70347, Q0P5X5, Q14B71, Q283Q6, Q29RT4, Q2M2Z5, Q3U0P1, Q3ULM6, Q3UXL4, Q3V089, Q571C7, Q5HZI1, Q5R9I1, Q5RD97, Q5W0B1, Q6GNV6, Q6KAQ7, Q6P1D7, Q6PDM4, Q7TSY8, Q7ZZH7, Q80WQ8, Q80YR6, Q86YC2, Q8BUH8

Diamond homologs: A4IFU8, D3ZUC4, E9Q6Z5, Q9H7T9

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

62 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	54
Likely benign	2
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

593 predictions. Top by Δscore:

Variant	Effect	Δscore
1:25837550:TGTGT:T	acceptor_gain	1.0000
1:25859288:T:A	donor_gain	1.0000
1:25835842:CTTTC:C	acceptor_gain	0.9900
1:25835845:TCCTA:T	acceptor_loss	0.9900
1:25835847:CT:C	acceptor_loss	0.9900
1:25837407:CCATA:C	donor_loss	0.9900
1:25837408:CATA:C	donor_loss	0.9900
1:25837409:ATACC:A	donor_loss	0.9900
1:25837410:TA:T	donor_loss	0.9900
1:25837412:C:G	donor_loss	0.9900
1:25837412:CCTGG:C	donor_gain	0.9900
1:25837551:GTGT:G	acceptor_gain	0.9900
1:25837555:C:CC	acceptor_gain	0.9900
1:25839019:TCA:T	donor_gain	0.9900
1:25846868:T:TA	donor_gain	0.9900
1:25859275:CCCA:C	donor_loss	0.9900
1:25859276:CCA:C	donor_loss	0.9900
1:25859277:CA:C	donor_loss	0.9900
1:25859278:AC:A	donor_loss	0.9900
1:25859287:TTCC:T	donor_gain	0.9900
1:25835843:TTTC:T	acceptor_gain	0.9800
1:25835847:C:CC	acceptor_gain	0.9800
1:25837551:GTGTC:G	acceptor_gain	0.9800
1:25837552:TGT:T	acceptor_gain	0.9800
1:25837552:TGTC:T	acceptor_loss	0.9800
1:25837552:TGTCT:T	acceptor_gain	0.9800
1:25837553:GT:G	acceptor_gain	0.9800
1:25837553:GTCT:G	acceptor_gain	0.9800
1:25837554:TC:T	acceptor_loss	0.9800
1:25837555:C:A	acceptor_loss	0.9800

AlphaMissense

2370 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
1:25859318:A:G	W14R	0.978
1:25859318:A:T	W14R	0.978
1:25835029:G:C	D346E	0.976
1:25835029:G:T	D346E	0.976
1:25859316:C:A	W14C	0.973
1:25859316:C:G	W14C	0.973
1:25835203:G:C	F288L	0.972
1:25835203:G:T	F288L	0.972
1:25835205:A:G	F288L	0.972
1:25835196:C:G	D291H	0.971
1:25835204:A:G	F288S	0.969
1:25835031:C:G	D346H	0.968
1:25835022:C:G	G349R	0.967
1:25835194:A:C	D291E	0.965
1:25835194:A:T	D291E	0.965
1:25835030:T:G	D346A	0.964
1:25859295:C:A	K21N	0.959
1:25859295:C:G	K21N	0.959
1:25835030:T:A	D346V	0.957
1:25835195:T:G	D291A	0.954
1:25859314:A:G	L15P	0.954
1:25835195:T:A	D291V	0.951
1:25835177:A:T	V297D	0.940
1:25835030:T:C	D346G	0.935
1:25835004:G:C	H355D	0.933
1:25835022:C:A	G349C	0.930
1:25837429:G:C	F68L	0.927
1:25837429:G:T	F68L	0.927
1:25837431:A:G	F68L	0.927
1:25859299:A:G	L20P	0.926

dbSNP variants (sampled 300 via entrez): RS1000034638 (1:25838657 C>T), RS1000106488 (1:25854076 T>A), RS1000317190 (1:25859621 C>T), RS1000335090 (1:25851848 C>T), RS1000474336 (1:25833703 G>A,T), RS1000503252 (1:25832922 A>C), RS1000529224 (1:25834098 T>C), RS1000572471 (1:25834127 A>T), RS1000637628 (1:25832672 C>T), RS1000943919 (1:25838497 C>A,G,T), RS1001010989 (1:25838743 A>C,T), RS1001246763 (1:25846399 A>G,T), RS1001794383 (1:25856036 A>G), RS1001868042 (1:25857003 C>A,T), RS1002095286 (1:25857060 G>A)

Disease associations

OMIM: gene MIM:620397 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

2 associations (top):

Study	Trait	p-value
GCST90002383_126	Hematocrit	3.000000e-11
GCST90002384_532	Hemoglobin	6.000000e-12

EFO canonical traits (2, from GWAS)

EFO ID	Trait name
EFO:0004348	hematocrit
EFO:0004509	hemoglobin measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

31 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	decreases expression, decreases methylation	2
Acetaminophen	decreases expression, increases expression	2
Valproic Acid	affects expression, decreases expression	2
3-((6-(2-methoxyphenyl)pyrimidin-4-yl)amino)phenyl)methane sulfonamide	decreases expression	1
afuresertib	decreases expression	1
lasiocarpine	increases expression	1
tris(1,3-dichloro-2-propyl)phosphate	affects expression	1
sodium arsenite	increases expression	1
perfluorooctanoic acid	decreases expression	1
manganese chloride	increases abundance, increases expression	1
benzo(e)pyrene	decreases methylation	1
2-(1H-indazol-4-yl)-6-(4-methanesulfonylpiperazin-1-ylmethyl)-4-morpholin-4-ylthieno(3,2-d)pyrimidine	decreases expression, increases response to substance	1
Temozolomide	increases expression	1
Sunitinib	decreases expression	1
Atrazine	decreases expression	1
Benzo(a)pyrene	increases expression	1
Calcitriol	decreases expression, affects cotreatment	1
Cisplatin	increases expression	1
Dichlorodiphenyl Dichloroethylene	increases expression	1
Estradiol	increases expression	1
Formaldehyde	decreases expression	1
Manganese	increases abundance, increases expression	1
Methapyrilene	decreases methylation	1
Oxygen	decreases expression	1
Testosterone	affects cotreatment, decreases expression	1
Tetrachlorodibenzodioxin	decreases expression	1
Tobacco Smoke Pollution	decreases expression	1
Tretinoin	decreases expression	1
Cyclosporine	increases expression	1
Aflatoxin B1	increases expression	1

Cellosaurus cell lines

2 cell lines: 2 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_E1RL	HAP1 AUNIP (-) 2	Cancer cell line	Male
CVCL_XL83	HAP1 AUNIP (-) 1	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.