AWAT2

Summary

AWAT2 (acyl-CoA wax alcohol acyltransferase 2, HGNC:23251) is a protein-coding gene on chromosome Xq13.1, encoding Acyl-CoA wax alcohol acyltransferase 2 (Q6E213). Acyltransferase that catalyzes the formation of ester bonds between fatty alcohols and fatty acyl-CoAs to form wax monoesters.

This gene encodes an enzyme belonging to the diacylglycerol acyltransferase family. This enzyme produces wax esters by the esterification of long chain (or wax) alcohols with acyl-CoA-derived fatty acids. It functions in lipid metabolism in the skin, mostly in undifferentiated peripheral sebocytes. This enzyme may also have acyl-CoA:retinol acyltransferase activities, where it catalyzes the synthesis of diacylglycerols and retinyl esters.

Source: NCBI Gene 158835 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 33 total — 1 pathogenic
• Druggable target: yes
• MANE Select transcript: NM_001002254

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:23251
Approved symbol	AWAT2
Name	acyl-CoA wax alcohol acyltransferase 2
Location	Xq13.1
Locus type	gene with protein product
Status	Approved
Aliases	MFAT
Ensembl gene	ENSG00000147160
Ensembl biotype	protein_coding
OMIM	300925
Entrez	158835

Gene structure

Transcript identifiers

Ensembl transcripts: 4 — 2 protein_coding, 1 nonsense_mediated_decay, 1 retained_intron

ENST00000276101, ENST00000440401, ENST00000443056, ENST00000949827

RefSeq mRNA: 1 — MANE Select: NM_001002254 NM_001002254

CCDS: CCDS35320

Canonical transcript exons

ENST00000276101 — 8 exons

Exon	Start	End
ENSE00000979015	70043926	70043996
ENSE00001163136	70044352	70044462
ENSE00001418270	70040542	70041622
ENSE00001463789	70049848	70049938
ENSE00001711291	70043069	70043243
ENSE00001796297	70042187	70042386
ENSE00003520941	70041773	70041962
ENSE00003642001	70043478	70043682

Expression profiles

Bgee: expression breadth broad, 28 present calls, max score 83.04.

Top tissues by expression

112 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
quadriceps femoris	UBERON:0001377	83.04	gold quality
cerebellar vermis	UBERON:0004720	82.54	gold quality
thymus	UBERON:0002370	82.10	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	70.80	gold quality
skin of abdomen	UBERON:0001416	60.98	gold quality
zone of skin	UBERON:0000014	56.51	gold quality
skin of leg	UBERON:0001511	53.13	gold quality
right testis	UBERON:0004534	49.60	gold quality
left testis	UBERON:0004533	47.41	gold quality
testis	UBERON:0000473	46.98	gold quality
sural nerve	UBERON:0015488	43.75	gold quality
right lung	UBERON:0002167	42.34	gold quality
ventricular zone	UBERON:0003053	40.62	silver quality
upper lobe of left lung	UBERON:0008952	38.80	gold quality
calcaneal tendon	UBERON:0003701	38.19	gold quality
bone marrow cell	CL:0002092	38.10	gold quality
lung	UBERON:0002048	37.89	gold quality
lower esophagus mucosa	UBERON:0035834	37.56	gold quality
ganglionic eminence	UBERON:0004023	37.54	silver quality
muscle tissue	UBERON:0002385	37.34	silver quality
colonic epithelium	UBERON:0000397	37.20	gold quality
cortical plate	UBERON:0005343	36.47	gold quality
skeletal muscle tissue	UBERON:0001134	35.28	gold quality
granulocyte	CL:0000094	34.93	gold quality
smooth muscle tissue	UBERON:0001135	34.90	silver quality
vermiform appendix	UBERON:0001154	34.74	gold quality
bone marrow	UBERON:0002371	34.27	silver quality
adrenal tissue	UBERON:0018303	33.36	gold quality
hindlimb stylopod muscle	UBERON:0004252	32.15	gold quality
tonsil	UBERON:0002372	32.13	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	2.13

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

61 targeting AWAT2, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-4283	100.00	66.42	2097
HSA-MIR-3925-3P	100.00	69.95	1237
HSA-MIR-340-5P	100.00	72.50	4437
HSA-MIR-3185	99.99	68.12	1959
HSA-MIR-4531	99.99	69.70	3181
HSA-MIR-185-3P	99.95	67.01	1743
HSA-MIR-3934-3P	99.76	65.51	1351
HSA-MIR-4524A-3P	99.72	66.85	2406
HSA-MIR-6762-3P	99.66	66.94	1188
HSA-MIR-6512-3P	99.65	66.07	1468
HSA-MIR-6720-5P	99.65	66.22	1459
HSA-MIR-1827	99.63	68.57	3265
HSA-MIR-9851-3P	99.63	69.68	1110
HSA-MIR-4516	99.61	67.78	3390
HSA-MIR-6716-5P	99.56	68.62	1244
HSA-MIR-4441	99.49	66.56	3216
HSA-MIR-889-5P	99.41	68.75	1025
HSA-MIR-4316	99.37	65.75	1360
HSA-MIR-593-5P	99.34	69.50	965
HSA-MIR-4506	99.34	67.47	526
HSA-MIR-4721	99.26	66.05	818
HSA-MIR-5584-3P	99.23	68.79	1351
HSA-MIR-1253	99.12	67.08	1688
HSA-MIR-4434	99.10	67.01	1984
HSA-MIR-5703	99.10	67.09	2053
HSA-MIR-4705	99.10	69.10	1091
HSA-MIR-371A-5P	99.08	66.51	1914
HSA-MIR-4717-3P	99.06	66.34	1072
HSA-MIR-670-3P	99.03	68.88	2404
HSA-MIR-922	99.02	67.23	1838

Literature-anchored findings (GeneRIF, showing 3)

• analysis of AWAT1 and AWAT2 substrate specificity and differentiation-specific expression pattern (PMID:15671038)
• MFAT is a multifunctional acyltransferase that likely plays an important role in lipid metabolism in human skin. (PMID:16106050)
• Expression of Acyl-CoA wax-alcohol acyltransferase 2 (AWAT2) by human and rabbit meibomian glands and meibocytes. (PMID:34838721)

Cross-species orthologs

9 orthologs

Organism	Symbol	Gene ID
mus_musculus	Awat2	ENSMUSG00000031220
rattus_norvegicus	Awat2	ENSRNOG00000003030
drosophila_melanogaster	CG1941	FBGN0033214
drosophila_melanogaster	Dgat2	FBGN0033215
drosophila_melanogaster	CG1946	FBGN0033216
caenorhabditis_elegans		WBGENE00010296
caenorhabditis_elegans		WBGENE00019464
caenorhabditis_elegans		WBGENE00020910
caenorhabditis_elegans		WBGENE00021818

Paralogs (6): DGAT2 (ENSG00000062282), MOGAT3 (ENSG00000106384), MOGAT1 (ENSG00000124003), MOGAT2 (ENSG00000166391), DGAT2L6 (ENSG00000184210), AWAT1 (ENSG00000204195)

Protein

Protein identifiers

Acyl-CoA wax alcohol acyltransferase 2 — Q6E213 (reviewed: Q6E213)

Alternative names: 11-cis-specific retinyl-ester synthase, Acyl-CoA retinol O-fatty-acyltransferase, Diacylglycerol O-acyltransferase 2-like protein 4, Diacylglycerol O-acyltransferase candidate 4, Long-chain-alcohol O-fatty-acyltransferase 2, Multifunctional O-acyltransferase, Wax synthase

All UniProt accessions (2): Q6E213, H0YAL0

UniProt curated annotations — full annotation on UniProt →

Function. Acyltransferase that catalyzes the formation of ester bonds between fatty alcohols and fatty acyl-CoAs to form wax monoesters. Shows a preference for medium chain acyl-CoAs from C12 to C16 in length and fatty alcohols shorter than C20, as the acyl donors and acceptors, respectively. Also possesses acyl-CoA retinol acyltransferase (ARAT) activity that preferentially esterifies 11-cis-retinol, a chromophore precursor of bleached opsin pigments in cone cells. Shows higher catalytic efficiency toward 11-cis-retinol versus 9-cis-retinol, 13-cis-retinol, and all-trans-retinol substrates.

Subunit / interactions. Monomer.

Subcellular location. Endoplasmic reticulum membrane.

Tissue specificity. Highly expressed in skin, where it is primarily restricted to undifferentiated peripheral sebocytes. Also expressed at lower level in other tissues except pancreas.

Activity regulation. 11-cis retinoids act as allosteric modulators of acyl-CoA retinol O-fatty-acyltransferase (ARAT) activity by suppressing esterification of 9-cis, 13-cis, or all-trans retinols concurrently increasing the enzyme specificity toward 11-cis isomer.

Similarity. Belongs to the diacylglycerol acyltransferase family.

RefSeq proteins (1): NP_001002254* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR007130	DAGAT	Family

Pfam: PF03982

Enzyme classification (BRENDA):

• EC 2.3.1.75 — long-chain-alcohol O-fatty-acyltransferase (BRENDA: 24 organisms, 473 substrates, 10 inhibitors, 48 Km, 37 kcat entries)

Substrate kinetics (BRENDA)

17 substrates with measured Km, best-characterized 15. Km ranges are aggregated across organisms/conditions.

Substrate	Km (mM)	Measurements
PALMITOYL-COA	0.0031–0.029	6
ARACHIDONOYL-COA	0.2263–0.2972	4
DECANOYL-COA	0.001–0.0176	4
EICOSAPENTAENOYL-COA	0.1016–0.1312	4
LAUROYL-COA	0.0001–0.0259	4
MYRISTOYL-COA	0.0039–0.0185	4
OCTADECATRIENOYL-COA	0.2809–0.2847	4
OCTANOYL-COA	0.0123–0.0299	4
STEAROYL-COA	0.0192–0.0759	4
11-CIS-RETINOL	0.0262	1
13-CIS-RETINOL	0.0352	1
9-CIS-RETINOL	0.0238	1
ALL-TRANS-RETINOL	0.0236	1
HEXADECANOL	0.044	1
HEXAN-1-OL	2.4	1

Catalyzed reactions (Rhea), 12 shown:

• an acyl-CoA + a 1,2-diacyl-sn-glycerol = a triacyl-sn-glycerol + CoA (RHEA:10868)
• all-trans-retinol + an acyl-CoA = an all-trans-retinyl ester + CoA (RHEA:11488)
• a 2-acylglycerol + an acyl-CoA = a 1,2-diacyl-sn-glycerol + CoA (RHEA:32947)
• 1-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoyl-CoA = 1,2-di-(9Z-octadecenoyl)-glycerol + CoA (RHEA:37915)
• 2-(9Z-octadecenoyl)-glycerol + hexadecanoyl-CoA = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CoA (RHEA:38071)
• 1,2-di-(9Z-octadecenoyl)-sn-glycerol + hexadecanoyl-CoA = 1,2-di-(9Z)-octadecenoyl-3-hexadecanoyl-sn-glycerol + CoA (RHEA:38163)
• hexadecan-1-ol + hexadecanoyl-CoA = hexadecyl hexadecanoate + CoA (RHEA:38167)
• hexadecane-1,2-diol + hexadecanoyl-CoA = 2-hydroxyhexadecyl hexadecanoate + CoA (RHEA:38171)
• all-trans-retinol + hexadecanoyl-CoA = all-trans-retinyl hexadecanoate + CoA (RHEA:38175)
• 1,2-di-(9Z-octadecenoyl)-sn-glycerol + (9Z)-octadecenoyl-CoA = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA (RHEA:38219)
• hexadecan-1-ol + (9Z)-octadecenoyl-CoA = hexadecyl (9Z)-octadecenoate + CoA (RHEA:38227)
• (9Z)-hexadecen-1-ol + (9Z)-octadecenoyl-CoA = 1-O-(9Z)-hexadecenyl (9Z)-octadecenoate + CoA (RHEA:38231)

UniProt features (4 total): transmembrane region 3, chain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q6E213-F1	94.34	0.86

Function

Pathways and Gene Ontology

Reactome pathways

10 pathways

ID	Pathway
R-HSA-1482883	Acyl chain remodeling of DAG and TAG
R-HSA-2187335	The retinoid cycle in cones (daylight vision)
R-HSA-9640463	Wax biosynthesis
R-HSA-1430728	Metabolism
R-HSA-1483206	Glycerophospholipid biosynthesis
R-HSA-1483257	Phospholipid metabolism
R-HSA-2187338	Visual phototransduction
R-HSA-556833	Metabolism of lipids
R-HSA-8848584	Wax and plasmalogen biosynthesis
R-HSA-9709957	Sensory Perception

MSigDB gene sets: 50 (showing top): GOBP_REGULATION_OF_HORMONE_LEVELS, GOBP_RETINOL_METABOLIC_PROCESS, ACEVEDO_LIVER_CANCER_WITH_H3K27ME3_UP, GOBP_GLYCEROLIPID_METABOLIC_PROCESS, GOBP_GLYCEROLIPID_BIOSYNTHETIC_PROCESS, GOBP_NEUTRAL_LIPID_BIOSYNTHETIC_PROCESS, GOBP_LIPID_METABOLIC_PROCESS, GOBP_NEUTRAL_LIPID_METABOLIC_PROCESS, GOBP_LIPID_BIOSYNTHETIC_PROCESS, GOBP_FATTY_ACID_DERIVATIVE_BIOSYNTHETIC_PROCESS, KEGG_GLYCEROLIPID_METABOLISM, GOBP_FATTY_ACID_DERIVATIVE_METABOLIC_PROCESS, GOBP_ISOPRENOID_METABOLIC_PROCESS, GOBP_PRIMARY_ALCOHOL_METABOLIC_PROCESS, GOBP_ALCOHOL_METABOLIC_PROCESS

GO Biological Process (6): retinoid metabolic process (GO:0001523), lipid metabolic process (GO:0006629), monoacylglycerol biosynthetic process (GO:0006640), wax biosynthetic process (GO:0010025), acylglycerol acyl-chain remodeling (GO:0036155), retinol metabolic process (GO:0042572)

GO Molecular Function (7): 2-acylglycerol O-acyltransferase activity (GO:0003846), diacylglycerol O-acyltransferase activity (GO:0004144), long-chain-alcohol O-fatty-acyltransferase activity (GO:0047196), retinol O-fatty-acyltransferase activity (GO:0050252), obsolete O-acyltransferase activity (GO:0008374), transferase activity (GO:0016740), acyltransferase activity (GO:0016746)

GO Cellular Component (3): endoplasmic reticulum membrane (GO:0005789), endoplasmic reticulum (GO:0005783), membrane (GO:0016020)

Reactome top-level categories

Rollup of top-7 pathways:

Category	Pathways
Metabolism of lipids	2
Glycerophospholipid biosynthesis	1
Visual phototransduction	1
Wax and plasmalogen biosynthesis	1
Phospholipid metabolism	1
Sensory Perception	1
Metabolism	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
acylglycerol O-acyltransferase activity	2
acyltransferase activity, transferring groups other than amino-acyl groups	2
diterpenoid metabolic process	1
primary metabolic process	1
monoacylglycerol metabolic process	1
acylglycerol biosynthetic process	1
wax metabolic process	1
fatty acid derivative biosynthetic process	1
acylglycerol metabolic process	1
retinoid metabolic process	1
primary alcohol metabolic process	1
hormone metabolic process	1
olefinic compound metabolic process	1
retinol metabolic process	1
catalytic activity	1
transferase activity	1
organelle membrane	1
nuclear outer membrane-endoplasmic reticulum membrane network	1
endoplasmic reticulum subcompartment	1
cytoplasm	1
endomembrane system	1
intracellular membrane-bounded organelle	1
cellular anatomical structure	1

Protein interactions and networks

STRING

590 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
AWAT2	ICAM1	P05362	892
AWAT2	ENY2	Q9NPA8	821
AWAT2	ZDHHC4	Q9NPG8	802
AWAT2	NSL1	Q96IY1	727
AWAT2	CMC2	Q9NRP2	666
AWAT2	PROCR	Q9UNN8	634
AWAT2	NXT2	Q9NPJ8	542
AWAT2	CD36	P16671	531
AWAT2	SCARB2	Q14108	528
AWAT2	SCARB1	Q8WTV0	524
AWAT2	FBXO8	Q9NRD0	523
AWAT2	FAM162A	Q96A26	507
AWAT2	ALB	P02768	458
AWAT2	FAR2	Q96K12	458
AWAT2	PECAM1	P16284	457

IntAct

2 interactions, top by confidence:

A	B	Type	Score
AWAT2	HPCAL1	psi-mi:“MI:0914”(association)	0.350

BioGRID (4): AWAT2 (Positive Genetic), UBE3A (Affinity Capture-MS), HERC3 (Affinity Capture-MS), HPCAL1 (Affinity Capture-MS)

ESM2 similar proteins: A0A1B0GVX0, A2ADU8, A2ADU9, A2Y075, A6QP72, O82232, P34655, P55017, P55018, P56508, P59158, P68178, P68179, Q0DKW8, Q0VBW2, Q22701, Q3ZCB2, Q53GD3, Q54RZ2, Q5REK4, Q5RJI2, Q5TYP8, Q66I68, Q6DK93, Q6DK99, Q6E1M8, Q6E213, Q6GMG8, Q6GZQ0, Q6NUC1, Q6P828, Q6PCW6, Q6ZPD8, Q8H5T6, Q8NG11, Q8QZY6, Q8S5M8, Q91VA1, Q9ARD5, Q9BYD5

Diamond homologs: A2ADU8, A2ADU9, A6QP72, K7K424, O74850, Q08650, Q28C88, Q2KHS5, Q3KPP4, Q3SYC2, Q4V9F0, Q54GC1, Q58HT5, Q5FVP8, Q5M7F4, Q5M8H5, Q6E1M8, Q6E213, Q6P342, Q6PAZ3, Q6ZPD8, Q70VZ7, Q70VZ8, Q75BY0, Q80W94, Q86VF5, Q91ZV4, Q96PD6, Q96PD7, Q96UY1, Q96UY2, Q9ASU1, Q9DCV3, A1A442, Q9ZVN2

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

33 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	1
Likely pathogenic	0
Uncertain significance	25
Likely benign	3
Benign	0

Top pathogenic / likely-pathogenic (1)

Variant ID	HGVS	Classification
1526803	GRCh37/hg19 Xq11.1-21.1(chrX:61877278-79123671)	Pathogenic

SpliceAI

1124 predictions. Top by Δscore:

Variant	Effect	Δscore
X:70041959:CCGA:C	acceptor_gain	1.0000
X:70041960:CGAC:C	acceptor_gain	1.0000
X:70041963:C:CC	acceptor_gain	1.0000
X:70041967:C:CT	acceptor_gain	1.0000
X:70041968:A:T	acceptor_gain	1.0000
X:70041976:CAG:C	acceptor_gain	1.0000
X:70041977:A:T	acceptor_gain	1.0000
X:70041978:G:C	acceptor_gain	1.0000
X:70041978:G:GC	acceptor_gain	1.0000
X:70042181:ACTC:A	donor_loss	1.0000
X:70042182:CT:C	donor_loss	1.0000
X:70042183:TCAC:T	donor_loss	1.0000
X:70042184:CACC:C	donor_loss	1.0000
X:70042185:A:AC	donor_gain	1.0000
X:70042186:C:CC	donor_gain	1.0000
X:70042186:C:CG	donor_loss	1.0000
X:70042319:C:CT	acceptor_gain	1.0000
X:70042358:C:CT	acceptor_gain	1.0000
X:70042358:C:T	acceptor_gain	1.0000
X:70042383:CACC:C	acceptor_gain	1.0000
X:70042385:CC:C	acceptor_gain	1.0000
X:70042386:CC:C	acceptor_gain	1.0000
X:70042387:C:CC	acceptor_gain	1.0000
X:70043241:CCC:C	acceptor_gain	1.0000
X:70043242:CCC:C	acceptor_gain	1.0000
X:70043683:C:CC	acceptor_gain	1.0000
X:70044346:GCTTA:G	donor_loss	1.0000
X:70044347:CTTAC:C	donor_loss	1.0000
X:70044348:TTA:T	donor_loss	1.0000
X:70044349:TACC:T	donor_loss	1.0000

AlphaMissense

2180 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
X:70041865:A:C	F315L	0.972
X:70041865:A:T	F315L	0.972
X:70041867:A:G	F315L	0.972
X:70042359:A:C	F225L	0.970
X:70042359:A:T	F225L	0.970
X:70042361:A:G	F225L	0.970
X:70043092:A:C	F208L	0.969
X:70043092:A:T	F208L	0.969
X:70043094:A:G	F208L	0.969
X:70041853:C:A	K319N	0.963
X:70041853:C:G	K319N	0.963
X:70043498:C:A	R151I	0.961
X:70043935:G:C	F86L	0.956
X:70043935:G:T	F86L	0.956
X:70043937:A:G	F86L	0.956
X:70043497:T:A	R151S	0.953
X:70043497:T:G	R151S	0.953
X:70041962:A:T	V283D	0.947
X:70043557:A:C	F131L	0.941
X:70043557:A:T	F131L	0.941
X:70043559:A:G	F131L	0.941
X:70043569:G:C	F127L	0.941
X:70043569:G:T	F127L	0.941
X:70043571:A:G	F127L	0.941
X:70042213:G:T	P274H	0.940
X:70044385:A:G	W55R	0.940
X:70044385:A:T	W55R	0.940
X:70041878:A:G	L311P	0.938
X:70043498:C:G	R151T	0.938
X:70042367:A:C	Y223D	0.937

dbSNP variants (sampled 300 via entrez): RS1000110599 (X:70051476 A>AC), RS1000279198 (X:70041475 C>T), RS1000405795 (X:70050187 G>A), RS1000797425 (X:70041131 G>A), RS1001570251 (X:70048407 T>G), RS1001786052 (X:70042237 G>A), RS1001957712 (X:70050728 C>T), RS1002308976 (X:70045492 G>A), RS1002915408 (X:70043862 C>A), RS1002961269 (X:70046742 T>G), RS1003213524 (X:70044249 C>A), RS1003777779 (X:70042756 G>A), RS1004189924 (X:70040149 A>G,T), RS1004244049 (X:70040667 G>A), RS1004370168 (X:70048818 G>A)

Disease associations

OMIM: gene MIM:300925 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL2375204 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

9 total (human), top 9 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	decreases methylation	1
Atrazine	increases expression	1
Benzo(a)pyrene	decreases methylation, increases methylation	1
Cadmium	decreases expression, increases abundance	1
Endosulfan	decreases expression	1
Phthalic Acids	decreases expression	1
Smoke	increases expression	1
Aflatoxin B1	increases methylation	1
Cadmium Chloride	decreases expression, increases abundance	1

ChEMBL screening assays

3 unique, capped per target: 3 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL2378804	Binding	Inhibition of human recombinant AWAT2 expressed in insect cell membrane using cetyl alcohol and [14C]oleoyl coenzyme A as substrate after 10 to 15 mins by liquid scintillation counting	Identification and design of a novel series of MGAT2 inhibitors. — Bioorg Med Chem Lett

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.