BCAR4

Summary

BCAR4 (breast cancer anti-estrogen resistance 4, HGNC:22170) is a long non-coding RNA gene on chromosome 16p13.13.

This gene produces a spliced long non-coding RNA (lncRNA) that has been implicated in breast cancer metastasis. It was originally identified in a screen for genes responsible for the development of resistance to anti-estrogens in breast cancer cells. It is thought that release of CCL21 enables this lncRNA to bind to the SNIP1 and PNUTS transcription factors, thereby activating a non-canonical GLI-dependent hedgehog signaling pathway that promotes cancer cell migration and invasion. A similar gene in cow expresses a protein in mature oocytes and preimplantation embryos. Alternatively spliced transcript variants have been identified.

Source: NCBI Gene 400500 — RefSeq curated summary.

At a glance

• Gene type: non-coding (lncRNA) — no protein product; not a drug target. Variant/disease associations are omitted (they would be positional, from an overlapping protein-coding gene).

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:22170
Approved symbol	BCAR4
Name	breast cancer anti-estrogen resistance 4
Location	16p13.13
Locus type	RNA, long non-coding
Status	Approved
Ensembl gene	ENSG00000262117
Ensembl biotype	lncRNA
OMIM	613746
Entrez	400500
RNAcentral	URS000075E333 — lncRNA, 1314 nt, 1 organism(s)

Gene structure

Transcript identifiers

Ensembl transcripts: 41 — 41 lncRNA

ENST00000571158, ENST00000571259, ENST00000573037, ENST00000573319, ENST00000574028, ENST00000577041, ENST00000615574, ENST00000725303, ENST00000725304, ENST00000725305, ENST00000725306, ENST00000725307, ENST00000725308, ENST00000725309, ENST00000725310, ENST00000725311, ENST00000725312, ENST00000725313, ENST00000725314, ENST00000725315, ENST00000725316, ENST00000725317, ENST00000725318, ENST00000725319, ENST00000725320, ENST00000725321, ENST00000725322, ENST00000725323, ENST00000725324, ENST00000725325, ENST00000725326, ENST00000725327, ENST00000725328, ENST00000725329, ENST00000725330, ENST00000725331, ENST00000725332, ENST00000725333, ENST00000725334, ENST00000725335, ENST00000725336

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

ENST00000571158 — 4 exons

Exon	Start	End
ENSE00002634757	11828322	11828391
ENSE00002683374	11821638	11821755
ENSE00004048796	11828699	11828868
ENSE00004048805	11819830	11820297

Expression profiles

Bgee: expression breadth ubiquitous, 117 present calls, max score 99.95.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 0.3423 / max 152.9764, expressed in 20 samples.

FANTOM5 promoters (3 alternative TSS)

Promoter ID	TPM avg	Samples expressed
156316	0.2952	17
156315	0.0270	10
156314	0.0201	8

Top tissues by expression

223 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
secondary oocyte	CL:0000655	99.95	gold quality
oocyte	CL:0000023	99.86	gold quality
placenta	UBERON:0001987	94.17	gold quality
decidua	UBERON:0002450	87.61	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	81.59	silver quality
tibialis anterior	UBERON:0001385	66.08	silver quality
buccal mucosa cell	CL:0002336	60.35	silver quality
stromal cell of endometrium	CL:0002255	59.85	gold quality
monocyte	CL:0000576	59.67	gold quality
pancreatic ductal cell	CL:0002079	58.91	silver quality
ileal mucosa	UBERON:0000331	58.48	silver quality
deltoid	UBERON:0001476	55.62	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	55.33	gold quality
epithelial cell of pancreas	CL:0000083	54.52	gold quality
gall bladder	UBERON:0002110	54.40	gold quality
islet of Langerhans	UBERON:0000006	54.38	gold quality
cardiac muscle of right atrium	UBERON:0003379	54.34	gold quality
left ventricle myocardium	UBERON:0006566	54.23	gold quality
kidney epithelium	UBERON:0004819	53.93	gold quality
upper arm skin	UBERON:0004263	53.52	gold quality
muscle of leg	UBERON:0001383	53.44	gold quality
sural nerve	UBERON:0015488	52.91	silver quality
gastrocnemius	UBERON:0001388	52.87	gold quality
right lobe of liver	UBERON:0001114	52.19	gold quality
female reproductive system	UBERON:0000474	51.08	gold quality
myocardium	UBERON:0002349	50.25	gold quality
pancreas	UBERON:0001264	50.16	gold quality
prefrontal cortex	UBERON:0000451	50.02	gold quality
left adrenal gland cortex	UBERON:0035825	49.93	gold quality
smooth muscle tissue	UBERON:0001135	49.64	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	2.93

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 24)

• findings showed showed that ectopic expression of BCAR4 caused 4-hydroxy-tamoxifen resistance and anchorage-independent cell growth in ZR-75-1 breast cancer cells and that the intact open reading frame was required for its function (PMID:16778085)
• BCAR4 induces tamoxifen resistance in breast cancer cell lines by activating ERBB2 signaling. (PMID:20859285)
• BCAR4 may have clinical relevance for tumor aggressiveness and tamoxifen resistance. (PMID:20859285)
• BCAR4 is a strong transforming gene causing estrogen-independent growth and antiestrogen resistance, and induces tumor formation in vivo. Due to its restricted expression, BCAR4 may be a good target for treating antiestrogen-resistant breast cancer. (PMID:21506106)
• Study reports a role for lncRNA BCAR4 in breast cancer metastasis that is mediated by chemokine-induced binding of BCAR4 to two transcription factors with extended regulatory consequences; this mechanism activates a noncanonical Hedgehog/GLI2 transcriptional program that promotes cell migration. (PMID:25416949)
• lncRNAs, like BCAR4, play roles in the regulation of Hedgehog/GLI 2 signal transduction pathways in cancer cells, which further broadens the scope of known lncRNA functions and is therapeutic target for the treatment of human cancers and other diseases. (PMID:26016563)
• Study shows that BCAR4 is highly expressed in breast cancer tissues, and encodes a functional protein driving the proliferation of endocrine-resistant breast cancer cells. (PMID:26317614)
• Overexpression of long non-coding RNA BCAR4 is associated with osteosarcoma progression. (PMID:27460090)
• upregulation of Long non-coding RNA BCAR4 expression plays a promoting role in the malignant progression of non-small cell lung cancer. (PMID:28077810)
• BCAR4 promoted chondrosarcoma cell proliferation and migration through activation of mTOR signaling pathway, and thus contributed to chondrosarcoma progression. (PMID:28399646)
• BCAR4 promotes the invasion and metastasis of non-small cell lung cancer cells via regulating epithelial-mesenchymal transition (PMID:28537678)
• long noncoding RNA breast cancer anti-estrogen resistance 4 (BCAR4) is required for YAP-dependent glycolysis. (PMID:28963395)
• The cooperative mechanism of lncRNA BCAR4 and GLI2 might provide a new opportunity for treating NSCLC. (PMID:29615150)
• High BCAR4 expression is associated with cervical cancer. (PMID:29937186)
• high BCAR4 expression was associated with clinical stage (PMID:30537165)
• lncRNA BCAR4 sponges miR3703p to promote bladder cancer progression via Wnt signaling. (PMID:31894304)
• LncRNA BCAR4 promotes liver cancer progression by upregulating ANAPC11 expression through sponging miR1261. (PMID:32319544)
• Long non-coding RNA BCAR4 accelerates cell proliferation and suppresses cell apoptosis in gastric cancer via regulating MAPK/ERK signaling. (PMID:32329841)
• Silence of lncRNA BCAR4 alleviates the deterioration of osteoporosis. (PMID:32572903)
• Variant in BCAR4 gene correlated with the breast cancer susceptibility and mRNA expression of lncRNA BCAR4 in Chinese Han population. (PMID:33113070)
• A fusion of CD63-BCAR4 identified in lung adenocarcinoma promotes tumorigenicity and metastasis. (PMID:33204025)
• Long non-coding RNA BCAR4 aggravated proliferation and migration in esophageal squamous cell carcinoma by negatively regulating p53/p21 signaling pathway. (PMID:33602031)
• The role of long non-coding RNA BCAR4 in human cancers. (PMID:34041673)
• Long Non-Coding RNA BCAR4 Promotes Oxaliplatin Resistance in Colorectal Cancer by Modulating miR-484-3p/RAB5C Expression. (PMID:36657426)

Cross-species orthologs

0 orthologs

Protein

Non-coding RNA — no protein product; not a drug target.

Function

No curated pathway, Gene-Ontology, or interaction data.

Disease & clinical

No curated disease, variant, or cancer-driver associations.

Drugs & pharmacology

No drug or pharmacology data — not an established drug target.