BCL2L13

Summary

BCL2L13 (BCL2 like 13, HGNC:17164) is a protein-coding gene on chromosome 22q11.21, encoding Bcl-2-like protein 13 (Q9BXK5). May promote the activation of caspase-3 and apoptosis.

This gene encodes a mitochondrially-localized protein with conserved B-cell lymphoma 2 homology motifs. Overexpression of the encoded protein results in apoptosis. Alternatively spliced transcript variants have been observed for this gene.

Source: NCBI Gene 23786 — RefSeq curated summary.

At a glance

• GWAS associations: 13
• Clinical variants (ClinVar): 97 total
• Druggable target: yes
• MANE Select transcript: NM_015367

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:17164
Approved symbol	BCL2L13
Name	BCL2 like 13
Location	22q11.21
Locus type	gene with protein product
Status	Approved
Aliases	MIL1, BCL-RAMBO
Ensembl gene	ENSG00000099968
Ensembl biotype	protein_coding
OMIM	619822
Entrez	23786

Gene structure

Transcript identifiers

Ensembl transcripts: 25 — 19 protein_coding, 4 protein_coding_CDS_not_defined, 2 nonsense_mediated_decay

ENST00000317582, ENST00000337612, ENST00000355028, ENST00000399777, ENST00000399781, ENST00000399782, ENST00000464649, ENST00000479296, ENST00000485631, ENST00000493680, ENST00000498133, ENST00000538149, ENST00000611738, ENST00000616863, ENST00000618481, ENST00000870789, ENST00000870790, ENST00000870791, ENST00000870792, ENST00000870793, ENST00000870794, ENST00000870795, ENST00000917411, ENST00000941948, ENST00000941949

RefSeq mRNA: 11 — MANE Select: NM_015367 NM_001270726, NM_001270727, NM_001270728, NM_001270729, NM_001270730, NM_001270731, NM_001270732, NM_001270734, NM_001270735, NM_001363824, NM_015367

CCDS: CCDS13746, CCDS59447, CCDS74810, CCDS74812, CCDS74813, CCDS74814, CCDS86995

Canonical transcript exons

ENST00000317582 — 7 exons

Exon	Start	End
ENSE00001845131	17638757	17638886
ENSE00003497481	17696141	17696210
ENSE00003591214	17688986	17689142
ENSE00003626534	17726677	17730855
ENSE00003693073	17683214	17683321
ENSE00003715933	17702243	17702386
ENSE00003723905	17655662	17655832

Expression profiles

Bgee: expression breadth ubiquitous, 287 present calls, max score 97.55.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 30.3167 / max 354.0669, expressed in 1817 samples.

FANTOM5 promoters (7 alternative TSS)

Promoter ID	TPM avg	Samples expressed
190985	16.6148	1808
190990	6.0487	1189
190987	3.4348	1462
190984	2.0402	1064
190989	1.1390	545
190986	0.9429	572
190991	0.0963	8

Top tissues by expression

288 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
secondary oocyte	CL:0000655	97.55	gold quality
oocyte	CL:0000023	96.51	gold quality
gastrocnemius	UBERON:0001388	96.47	gold quality
middle temporal gyrus	UBERON:0002771	96.29	gold quality
muscle of leg	UBERON:0001383	96.25	gold quality
hindlimb stylopod muscle	UBERON:0004252	95.53	gold quality
muscle organ	UBERON:0001630	95.45	gold quality
gluteal muscle	UBERON:0002000	95.05	gold quality
skeletal muscle tissue of rectus abdominis	UBERON:0004511	94.80	gold quality
islet of Langerhans	UBERON:0000006	94.50	gold quality
skeletal muscle tissue	UBERON:0001134	94.41	gold quality
Brodmann (1909) area 23	UBERON:0013554	94.38	gold quality
tibialis anterior	UBERON:0001385	94.25	gold quality
deltoid	UBERON:0001476	94.09	gold quality
diaphragm	UBERON:0001103	94.02	gold quality
adrenal tissue	UBERON:0018303	94.02	gold quality
substantia nigra pars compacta	UBERON:0001965	93.45	gold quality
palpebral conjunctiva	UBERON:0001812	93.27	gold quality
muscle tissue	UBERON:0002385	93.17	gold quality
adrenal gland	UBERON:0002369	93.10	gold quality
left adrenal gland	UBERON:0001234	93.00	gold quality
quadriceps femoris	UBERON:0001377	93.00	gold quality
vastus lateralis	UBERON:0001379	92.98	gold quality
skeletal muscle tissue of biceps brachii	UBERON:0004502	92.96	gold quality
right adrenal gland cortex	UBERON:0035827	92.95	gold quality
right adrenal gland	UBERON:0001233	92.91	gold quality
substantia nigra pars reticulata	UBERON:0001966	92.88	gold quality
heart left ventricle	UBERON:0002084	92.73	gold quality
biceps brachii	UBERON:0001507	92.68	gold quality
calcaneal tendon	UBERON:0003701	92.67	gold quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 3.

Experiment	Marker?	Max mean expression
E-MTAB-7316	yes	35.70
E-GEOD-137537	yes	14.42
E-ANND-3	yes	5.50

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): FOXO1, ZIC1

miRNA regulators (miRDB)

112 targeting BCL2L13, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-4795-3P	100.00	74.62	4024
HSA-MIR-4533	100.00	69.48	2758
HSA-MIR-3667-3P	99.99	67.17	1636
HSA-MIR-548C-3P	99.99	74.01	7587
HSA-MIR-10401-5P	99.99	65.79	948
HSA-MIR-6870-5P	99.99	68.55	2115
HSA-MIR-3148	99.97	75.06	6478
HSA-MIR-4723-5P	99.97	68.70	2034
HSA-MIR-5698	99.97	68.49	2029
HSA-MIR-7111-5P	99.97	68.48	2062
HSA-MIR-570-3P	99.96	72.41	4910
HSA-MIR-141-3P	99.94	72.79	2421
HSA-MIR-200A-3P	99.94	72.68	2420
HSA-MIR-6835-3P	99.93	70.49	2904
HSA-MIR-6721-5P	99.93	68.92	2981
HSA-MIR-589-3P	99.91	69.62	2088
HSA-MIR-627-3P	99.90	71.42	3316
HSA-MIR-124-3P	99.89	73.74	3043
HSA-MIR-506-3P	99.89	73.55	3057
HSA-MIR-3919	99.87	69.45	2489
HSA-MIR-4492	99.87	68.25	3611
HSA-MIR-182-5P	99.87	74.03	2589
HSA-MIR-5003-3P	99.85	69.29	2517
HSA-MIR-6875-3P	99.82	70.26	2983
HSA-MIR-6842-5P	99.80	67.54	1587
HSA-MIR-7110-5P	99.80	67.84	1712
HSA-MIR-4668-5P	99.79	70.58	3782
HSA-MIR-4319	99.76	69.83	2586
HSA-MIR-199A-3P	99.75	70.48	929
HSA-MIR-199B-3P	99.75	70.48	929

Literature-anchored findings (GeneRIF, showing 14)

• Our data support the speculation that the Alu element insertion during the splicing process may play an important role in the generation of protein diversity in primate cells by a yet uncharacterized mechanism (PMID:12527362)
• SidF contributes to apoptosis resistance in L. pneumophila-infected cells by specifically interacting with and neutralizing the effects of BNIP3 and Bcl-rambo, two proapoptotic members of Bcl2 protein family. (PMID:17360363)
• investigated the expression of three apoptosis related genes, BCL2L13, CASP8AP2, and Livin, as well as their prognostic significance, in a retrospective study of 90 pediatric ALL patients diagnosed between 1996 and 2007 in Taiwan (PMID:20109966)
• Bcl-rambo is a pro-apoptotic member of the Bcl-2 family, inducing the permeability transition via interaction with the adenine nucleotide translocator (ANT). (PMID:22921587)
• Bcl2L13 binds to proapoptotic ceramide synthases 2 (CerS2) and 6 (CerS6) via a unique C-terminal 250-aa sequence located between its Bcl-2 homology and membrane anchor domains and blocks homo- and heteromeric CerS2/6 complex formation and activity. (PMID:24706805)
• This study showed that Bcl-2-like protein 13 (Bcl2-L-13) induces mitochondrial fragmentation and mitophagy in mammalian cells. (PMID:26146385)
• Ectopically expressed in Drosophila Bcl-rambo localizes to mitochondria, induces apoptosis and atrophied wing, split thorax, and rough eye phenotypes. (PMID:27348811)
• Bcl2-L-13 recruits the ULK1 complex to process mitophagy. (PMID:30625316)
• Human Bcl-rambo and Voltage-dependent anion channels cooperatively promote the activation of effector caspases in human cultured cells. (PMID:31102594)
• Long non-coding RNA SNHG15 is a competing endogenous RNA of miR-141-3p that prevents osteoarthritis progression by upregulating BCL2L13 expression. (PMID:32247266)
• Selective binding of mitophagy receptor protein Bcl-rambo to LC3/GABARAP family proteins. (PMID:32828302)
• LncRNA SNHG14 is beneficial to oxygen glucose deprivation/reoxygenation-induced neuro-2a cell injury via mir-98-5p sequestration-caused BCL2L13 upregulation. (PMID:35678981)
• The BCL-2 family protein BCL-RAMBO interacts and cooperates with GRP75 to promote its apoptosis signaling pathway. (PMID:37640805)
• The role of BCL2L13 in glioblastoma: turning a need into a target. (PMID:37988705)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	bcl2l13	ENSDARG00000062370
danio_rerio	si:ch211-214j24.14	ENSDARG00000096037
mus_musculus	Bcl2l13	ENSMUSG00000009112
rattus_norvegicus	Bcl2l13	ENSRNOG00000012394

Protein

Protein identifiers

Bcl-2-like protein 13 — Q9BXK5 (reviewed: Q9BXK5)

Alternative names: Bcl-rambo, Protein Mil1

All UniProt accessions (9): Q9BXK5, A0A087WTL4, A0A087WW80, A0A087WX97, A0A0A0MR90, E9PDD6, F2Z2C3, F6VJB4, H0Y3R5

UniProt curated annotations — full annotation on UniProt →

Function. May promote the activation of caspase-3 and apoptosis.

Subunit / interactions. Monomer.

Subcellular location. Mitochondrion membrane. Nucleus Nucleus.

Tissue specificity. Ubiquitous, with the highest levels of expression in heart, placenta and pancreas.

Similarity. Belongs to the Bcl-2 family.

Isoforms (3)

UniProt ID	Names	Canonical?
Q9BXK5-1	2	yes
Q9BXK5-2	1
Q9BXK5-4	3

RefSeq proteins (11): NP_001257655, NP_001257656, NP_001257657, NP_001257658, NP_001257659, NP_001257660, NP_001257661, NP_001257663, NP_001257664, NP_001350753, NP_056182* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR002475	Bcl2-like	Family
IPR036834	Bcl-2-like_sf	Homologous_superfamily
IPR042398	BCL2L13	Family
IPR046371	Bcl-2_BH1-3	Domain

Pfam: PF00452

UniProt features (34 total): modified residue 12, short sequence motif 4, repeat 4, sequence variant 4, splice variant 3, sequence conflict 2, region of interest 2, chain 1, transmembrane region 1, compositionally biased region 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9BXK5-F1	65.27	0.32

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (12): 38, 259, 261, 303, 326, 371, 375, 410, 420, 426, 429, 444

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 256 (showing top): GOBP_CARDIAC_CHAMBER_DEVELOPMENT, GOBP_NUCLEOSIDE_DIPHOSPHATE_METABOLIC_PROCESS, GOBP_MUSCLE_TISSUE_DEVELOPMENT, GOBP_CARDIAC_LEFT_VENTRICLE_MORPHOGENESIS, MODULE_255, GOBP_CARDIAC_CHAMBER_MORPHOGENESIS, ACEVEDO_NORMAL_TISSUE_ADJACENT_TO_LIVER_TUMOR_DN, GOBP_CARBOHYDRATE_DERIVATIVE_CATABOLIC_PROCESS, MODULE_317, GOBP_MONOSACCHARIDE_CATABOLIC_PROCESS, CREBP1_Q2, GOBP_ORGANOPHOSPHATE_METABOLIC_PROCESS, TGACCTY_ERR1_Q2, GOBP_NUCLEOSIDE_PHOSPHATE_BIOSYNTHETIC_PROCESS, GOBP_MONOCARBOXYLIC_ACID_METABOLIC_PROCESS

GO Biological Process (7): mitophagy (GO:0000423), oxidative phosphorylation (GO:0006119), apoptotic process (GO:0006915), mitochondrion organization (GO:0007005), regulation of apoptotic process (GO:0042981), fat cell differentiation (GO:0045444), canonical glycolysis (GO:0061621)

GO Molecular Function (2): cysteine-type endopeptidase activator activity involved in apoptotic process (GO:0008656), protein binding (GO:0005515)

GO Cellular Component (4): nucleus (GO:0005634), mitochondrion (GO:0005739), membrane (GO:0016020), mitochondrial membrane (GO:0031966)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
intracellular membrane-bounded organelle	2
autophagy of mitochondrion	1
macroautophagy	1
aerobic respiration	1
proton motive force-driven ATP synthesis	1
programmed cell death	1
apoptotic signaling pathway	1
execution phase of apoptosis	1
organelle organization	1
apoptotic process	1
regulation of programmed cell death	1
cell differentiation	1
glucokinase activity	1
glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity	1
glucose catabolic process	1
glycolytic process through glucose-6-phosphate	1
peptidase activator activity involved in apoptotic process	1
cysteine-type endopeptidase regulator activity involved in apoptotic process	1
binding	1
cytoplasm	1
cellular anatomical structure	1
mitochondrion	1
mitochondrial envelope	1
organelle membrane	1

Protein interactions and networks

STRING

1024 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
BCL2L13	GABARAP	O95166	932
BCL2L13	FUNDC1	Q8IVP5	893
BCL2L13	BNIP3	Q12983	822
BCL2L13	BNIP3L	O60238	817
BCL2L13	DNM1L	O00429	810
BCL2L13	FKBP8	Q14318	809
BCL2L13	CD300C	Q08708	768
BCL2L13	CERS2	Q96G23	701
BCL2L13	F5GZY7	F5GZY7	693
BCL2L13	PHB2	Q99623	688
BCL2L13	CALCOCO2	Q13137	685
BCL2L13	OPTN	Q96CV9	669
BCL2L13	AMBRA1	Q9C0C7	665
BCL2L13	GABARAPL2	P60520	662
BCL2L13	NBR1	Q14596	645

IntAct

241 interactions, top by confidence:

A	B	Type	Score
TMEM86B	BCL2L13	psi-mi:“MI:0915”(physical association)	0.720
PLN	BCL2L13	psi-mi:“MI:0915”(physical association)	0.720
PLP1	BCL2L13	psi-mi:“MI:0915”(physical association)	0.720
GABARAPL2	BCL2L13	psi-mi:“MI:0915”(physical association)	0.670
sidF	BCL2L13	psi-mi:“MI:0915”(physical association)	0.590
sidF	BCL2L13	psi-mi:“MI:0407”(direct interaction)	0.590
BCL2L13	SOD1	psi-mi:“MI:0915”(physical association)	0.590
	BCL2L13	psi-mi:“MI:0915”(physical association)	0.560
BCL2L13	VAMP4	psi-mi:“MI:0915”(physical association)	0.560
BCL2L13	VAMP3	psi-mi:“MI:0915”(physical association)	0.560
BCL2L13	BET1	psi-mi:“MI:0915”(physical association)	0.560
BCL2L13	TMEM128	psi-mi:“MI:0915”(physical association)	0.560
TMUB2	BCL2L13	psi-mi:“MI:0915”(physical association)	0.560
BCL2L13	NINJ2	psi-mi:“MI:0915”(physical association)	0.560

BioGRID (167): BCL2L13 (Two-hybrid), BCL2L13 (Two-hybrid), BCL2L13 (Two-hybrid), BCL2L13 (Two-hybrid), BCL2L13 (Two-hybrid), BCL2L13 (Two-hybrid), PLLP (Two-hybrid), TMUB2 (Two-hybrid), TMEM128 (Two-hybrid), TMEM86B (Two-hybrid), BCL2L13 (Two-hybrid), BCL2L13 (Two-hybrid), SOD1 (Affinity Capture-MS), BCL2L13 (Affinity Capture-MS), BCL2L13 (Two-hybrid)

ESM2 similar proteins: A1A4V9, A2A8U2, A4IFI1, A8E4X8, B0BMZ6, F1R7R1, G5E8P0, O75161, P12755, P59017, P59240, P85299, P97432, Q14DQ1, Q2HJA5, Q3B7M3, Q3U0L2, Q3ZBK7, Q3ZK22, Q53GS7, Q569K6, Q58DT5, Q5FVG6, Q5RAS2, Q5SNT2, Q5T7N3, Q5XI52, Q60698, Q6NZQ0, Q80U62, Q812A5, Q8C0R7, Q8C190, Q8CC12, Q8IWY9, Q8IYY4, Q8N9B5, Q8NFW9, Q8R1F1, Q8R322

Diamond homologs: P59017, Q9BXK5

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

97 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	74
Likely benign	8
Benign	2

Top pathogenic / likely-pathogenic (0)

SpliceAI

1823 predictions. Top by Δscore:

Variant	Effect	Δscore
22:17655658:TTAG:T	acceptor_loss	1.0000
22:17655659:TAGG:T	acceptor_loss	1.0000
22:17655660:A:AG	acceptor_gain	1.0000
22:17655660:AGGTT:A	acceptor_loss	1.0000
22:17655661:G:GC	acceptor_loss	1.0000
22:17655661:G:GG	acceptor_gain	1.0000
22:17655828:CCAAG:C	donor_loss	1.0000
22:17655829:CAAGG:C	donor_loss	1.0000
22:17655830:AAGG:A	donor_loss	1.0000
22:17655831:AGGTA:A	donor_loss	1.0000
22:17655832:GGTA:G	donor_loss	1.0000
22:17655834:T:G	donor_loss	1.0000
22:17683209:TTCA:T	acceptor_loss	1.0000
22:17683211:CAGG:C	acceptor_loss	1.0000
22:17683212:A:AG	acceptor_gain	1.0000
22:17683212:AG:A	acceptor_gain	1.0000
22:17683212:AGG:A	acceptor_gain	1.0000
22:17683212:AGGG:A	acceptor_gain	1.0000
22:17683213:G:A	acceptor_gain	1.0000
22:17683213:G:GA	acceptor_gain	1.0000
22:17683213:GGG:G	acceptor_gain	1.0000
22:17683213:GGGG:G	acceptor_gain	1.0000
22:17683213:GGGGT:G	acceptor_gain	1.0000
22:17683317:TGAAG:T	donor_loss	1.0000
22:17683319:AAGGT:A	donor_loss	1.0000
22:17683320:AGG:A	donor_loss	1.0000
22:17683321:GG:G	donor_loss	1.0000
22:17683323:T:G	donor_loss	1.0000
22:17689139:GCCA:G	donor_gain	1.0000
22:17689143:G:GG	donor_gain	1.0000

AlphaMissense

3126 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
22:17702384:T:A	W200R	0.997
22:17702384:T:C	W200R	0.997
22:17726979:C:A	N301K	0.997
22:17726979:C:G	N301K	0.997
22:17726902:T:A	W276R	0.995
22:17726902:T:C	W276R	0.995
22:17726976:C:A	N300K	0.995
22:17726976:C:G	N300K	0.995
22:17689028:T:C	F91S	0.994
22:17702386:G:C	W200C	0.994
22:17702386:G:T	W200C	0.994
22:17726968:A:C	S298R	0.994
22:17726970:T:A	S298R	0.994
22:17726970:T:G	S298R	0.994
22:17726988:T:A	N304K	0.994
22:17726988:T:G	N304K	0.994
22:17689003:T:C	F83L	0.993
22:17689005:T:A	F83L	0.993
22:17689005:T:G	F83L	0.993
22:17655764:T:A	V18D	0.992
22:17726904:G:C	W276C	0.992
22:17726904:G:T	W276C	0.992
22:17655767:T:A	V19D	0.991
22:17689025:T:A	V90E	0.991
22:17689027:T:C	F91L	0.991
22:17689029:C:A	F91L	0.991
22:17689029:C:G	F91L	0.991
22:17726975:A:T	N300I	0.991
22:17655742:T:C	F11L	0.990
22:17655744:T:A	F11L	0.990

dbSNP variants (sampled 300 via entrez): RS1000000965 (22:17630228 A>AC), RS1000031146 (22:17694116 A>T), RS1000046453 (22:17687927 C>A,T), RS1000047683 (22:17650143 T>A), RS1000110328 (22:17655531 T>A), RS1000111151 (22:17642038 A>G), RS1000111639 (22:17674586 A>G), RS1000114120 (22:17685381 A>T), RS1000187602 (22:17701631 A>C), RS1000189542 (22:17723648 C>T), RS1000191738 (22:17671661 C>A), RS1000196439 (22:17634474 C>T), RS1000278044 (22:17688107 C>T), RS1000311178 (22:17634190 T>G), RS1000311934 (22:17671494 G>A)

Disease associations

OMIM: gene MIM:619822 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

13 associations (top):

Study	Trait	p-value
GCST004613_95	Sum neutrophil eosinophil counts	3.000000e-09
GCST004614_53	Granulocyte count	3.000000e-09
GCST004626_164	Myeloid white cell count	6.000000e-10
GCST004629_51	Neutrophil count	8.000000e-09
GCST005925_4	Baseline cortisol levels in response to low dose short synacthen test in corticosteroid treated asthma	2.000000e-08
GCST006102_5	Interleukin-10 levels	2.000000e-09
GCST010083_357	Hemoglobin levels	1.000000e-08
GCST90002381_439	Eosinophil count	2.000000e-12
GCST90002389_434	Lymphocyte percentage of white cells	1.000000e-16
GCST90002393_480	Monocyte count	3.000000e-09
GCST90002398_72	Neutrophil count	6.000000e-24
GCST90002399_98	Neutrophil percentage of white cells	3.000000e-12
GCST90002407_188	White blood cell count	1.000000e-20

EFO canonical traits (10, from GWAS)

EFO ID	Trait name
EFO:0004833	neutrophil count
EFO:0004842	eosinophil count
EFO:0007987	granulocyte count
EFO:0005843	cortisol measurement
EFO:0009175	response to synacthen
EFO:0004750	interleukin 10 measurement
EFO:0004509	hemoglobin measurement
EFO:0007993	lymphocyte percentage of leukocytes
EFO:0005091	monocyte count
EFO:0007990	neutrophil percentage of leukocytes

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067078 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
6.58	Kd	261.9	nM	CHEMBL5653589
6.58	ED50	261.9	nM	CHEMBL5653589

PubChem BioAssay actives

1 with measured affinity, of 2 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2147944: Binding affinity to human BCL2L13 incubated for 45 mins by Kinobead based pull down assay	kd	0.2619	uM

CTD chemical–gene interactions

51 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	decreases expression, increases expression, affects cotreatment	3
GSK-J4	decreases expression	1
FR900359	affects phosphorylation	1
bisphenol F	affects cotreatment, increases expression	1
triphenyl phosphate	affects expression	1
deoxynivalenol	increases expression	1
potassium perchlorate	decreases expression	1
sodium arsenate	decreases expression	1
sodium arsenite	decreases expression	1
coumarin	affects phosphorylation	1
CGP 52608	affects binding, increases reaction	1
deguelin	decreases expression	1
fenpyroximate	decreases expression	1
4-chloro-N-((4-(1,1-dimethylethyl)phenyl)methyl)-3-ethyl-1-methyl-1H-pyrazole-5-carboxamide	decreases expression	1
oxidized-L-alpha-1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine	affects expression, increases reaction	1
pyrimidifen	decreases expression	1
abrine	increases expression	1
pyrachlostrobin	decreases expression	1
bisphenol S	increases expression	1
picoxystrobin	decreases expression	1
duvelisib	increases expression	1
Resveratrol	affects cotreatment, increases expression	1
Acetaminophen	increases expression	1
Antimycin A	decreases expression	1
Atrazine	decreases expression	1
Vehicle Emissions	affects expression, increases reaction	1
Azacitidine	increases expression	1
Cannabidiol	decreases expression	1
Dexamethasone	affects cotreatment, increases expression	1
Diazinon	increases methylation	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5650986	Binding	Binding affinity to human BCL2L13 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Cellosaurus cell lines

1 cell lines: 1 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_D7KX	Ubigene A-549 BCL2L13 KO	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.