BHMT2

Summary

BHMT2 (betaine–homocysteine S-methyltransferase 2, HGNC:1048) is a protein-coding gene on chromosome 5q14.1, encoding S-methylmethionine–homocysteine S-methyltransferase BHMT2 (Q9H2M3). Involved in the regulation of homocysteine metabolism.

Homocysteine is a sulfur-containing amino acid that plays a crucial role in methylation reactions. Transfer of the methyl group from betaine to homocysteine creates methionine, which donates the methyl group to methylate DNA, proteins, lipids, and other intracellular metabolites. The protein encoded by this gene is one of two methyl transferases that can catalyze the transfer of the methyl group from betaine to homocysteine. Anomalies in homocysteine metabolism have been implicated in disorders ranging from vascular disease to neural tube birth defects such as spina bifida. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Source: NCBI Gene 23743 — RefSeq curated summary.

At a glance

• GWAS associations: 17
• Clinical variants (ClinVar): 69 total
• Druggable target: yes
• MANE Select transcript: NM_017614

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:1048
Approved symbol	BHMT2
Name	betaine–homocysteine S-methyltransferase 2
Location	5q14.1
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000132840
Ensembl biotype	protein_coding
OMIM	605932
Entrez	23743

Gene structure

Transcript identifiers

Ensembl transcripts: 17 — 13 protein_coding, 3 retained_intron, 1 nonsense_mediated_decay

ENST00000255192, ENST00000518666, ENST00000518758, ENST00000519743, ENST00000521567, ENST00000523046, ENST00000523472, ENST00000896177, ENST00000896178, ENST00000896179, ENST00000896180, ENST00000896181, ENST00000896182, ENST00000896183, ENST00000896184, ENST00000896185, ENST00000896186

RefSeq mRNA: 2 — MANE Select: NM_017614 NM_001178005, NM_017614

CCDS: CCDS4045, CCDS54871

Canonical transcript exons

ENST00000255192 — 8 exons

Exon	Start	End
ENSE00000971653	79083192	79083374
ENSE00002115751	79088493	79090069
ENSE00002505369	79083628	79083856
ENSE00003561740	79077480	79077612
ENSE00003561989	79069767	79069815
ENSE00003640609	79080687	79080878
ENSE00003684127	79082809	79082956
ENSE00003694684	79079369	79079460

Expression profiles

Bgee: expression breadth ubiquitous, 237 present calls, max score 99.16.

FANTOM5 (CAGE): breadth broad, TPM avg 4.6244 / max 515.7169, expressed in 596 samples.

FANTOM5 promoters (4 alternative TSS)

Promoter ID	TPM avg	Samples expressed
57230	3.1159	417
57229	0.7740	321
57227	0.6726	336
57228	0.0619	32

Top tissues by expression

286 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
renal medulla	UBERON:0000362	99.16	gold quality
right lobe of liver	UBERON:0001114	99.11	gold quality
liver	UBERON:0002107	98.67	gold quality
adult mammalian kidney	UBERON:0000082	98.63	gold quality
metanephros cortex	UBERON:0010533	97.95	gold quality
nephron tubule	UBERON:0001231	97.50	gold quality
kidney epithelium	UBERON:0004819	96.52	gold quality
adult organism	UBERON:0007023	96.32	gold quality
kidney	UBERON:0002113	96.11	gold quality
lower esophagus muscularis layer	UBERON:0035833	95.58	gold quality
lower esophagus	UBERON:0013473	95.52	gold quality
caput epididymis	UBERON:0004358	95.23	gold quality
renal glomerulus	UBERON:0000074	93.98	gold quality
mucosa of stomach	UBERON:0001199	93.51	gold quality
right lobe of thyroid gland	UBERON:0001119	93.48	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	93.47	gold quality
metanephric glomerulus	UBERON:0004736	93.45	gold quality
descending thoracic aorta	UBERON:0002345	93.43	gold quality
cortex of kidney	UBERON:0001225	92.85	gold quality
left lobe of thyroid gland	UBERON:0001120	92.71	gold quality
corpus epididymis	UBERON:0004359	92.28	gold quality
tibial nerve	UBERON:0001323	91.60	gold quality
right adrenal gland cortex	UBERON:0035827	91.54	gold quality
thyroid gland	UBERON:0002046	91.48	gold quality
subcutaneous adipose tissue	UBERON:0002190	91.38	gold quality
metanephros	UBERON:0000081	91.15	gold quality
stromal cell of endometrium	CL:0002255	90.76	gold quality
adipose tissue	UBERON:0001013	90.75	gold quality
thoracic aorta	UBERON:0001515	90.70	gold quality
left adrenal gland	UBERON:0001234	90.58	gold quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 2.

Experiment	Marker?	Max mean expression
E-CURD-119	yes	46.15
E-CURD-135	no	1573.07
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

55 targeting BHMT2, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3064-3P	100.00	70.09	1254
HSA-MIR-548C-3P	99.99	74.01	7587
HSA-MIR-4531	99.99	69.70	3181
HSA-MIR-568	99.98	69.86	2084
HSA-MIR-6778-3P	99.96	67.29	2693
HSA-MIR-3605-5P	99.96	67.12	932
HSA-MIR-205-3P	99.92	69.92	3165
HSA-MIR-627-3P	99.90	71.42	3316
HSA-MIR-153-5P	99.89	73.86	6317
HSA-MIR-4492	99.87	68.25	3611
HSA-MIR-5582-3P	99.86	72.48	4221
HSA-MIR-3121-3P	99.82	71.96	3630
HSA-MIR-548M	99.70	68.87	1749
HSA-MIR-548BA	99.69	69.14	1514
HSA-MIR-3202	99.66	67.70	2737
HSA-MIR-6132	99.60	65.83	1554
HSA-MIR-6836-5P	99.60	65.62	1538
HSA-MIR-7152-5P	99.60	69.33	2094
HSA-MIR-762	99.58	66.61	1994
HSA-MIR-4498	99.47	67.42	2360
HSA-MIR-6165	99.44	67.12	1389
HSA-MIR-501-3P	99.33	66.12	651
HSA-MIR-502-3P	99.33	66.12	651
HSA-MIR-5190	99.15	67.76	1234
HSA-MIR-6510-5P	99.14	66.59	1081
HSA-MIR-1253	99.12	67.08	1688
HSA-MIR-5001-5P	99.05	66.76	1972
HSA-MIR-6506-5P	99.04	65.66	1386
HSA-MIR-7151-3P	99.04	69.72	2370
HSA-MIR-511-5P	98.97	70.94	2268

Literature-anchored findings (GeneRIF, showing 10)

• Hyperhomocysteinemia, a risk factor for coronary diseases, can be caused by genetic mutations in BHMT2 metabolism. (PMID:12818402)
• Betaine-homocysteine S-methyltransferase-2 is an S-methylmethionine-homocysteine methyltransferase. (PMID:18230605)
• Common genetic variation in BHMT and BHMT2 and functionally characterized BHMT SNPs. (PMID:18457970)
• gene-gene interaction analysis revealed a significant epistatic interaction of BHMT2 (rs673752), PEMT (rs12325817), and PCYT1A (rs712012) with maternal NCL/P susceptibility. (PMID:20662904)
• No significant level of association was found with cleft lip with or without cleft palate and BHMT2 variants. (PMID:21564312)
• Multiple SNPs in BHMT and BHMT2 were identified to be associated with the occurrence of infant obstructive heart defects and interaction effects with maternal use of folic acid supplements. (PMID:25846410)
• The faster evolutionary rate of BHMT2 overall suggests that selective constraints were reduced relative to BHMT. (PMID:26213999)
• A three-way interaction among maternal and fetal variants in BHMT2, GSTP1 and GPX3 contribute to congenital heart defects (PMID:26612412)
• Our study suggested markers in BHMT/BHMT2 and DMGDH might affect the risk of NSCL/P through pairwise interaction. (PMID:29356306)
• Association of Maternal Betaine-Homocysteine Methyltransferase (BHMT) and BHMT2 Genes Polymorphisms with Congenital Heart Disease in Offspring. (PMID:35835902)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	bhmt	ENSDARG00000013430
mus_musculus	Bhmt2	ENSMUSG00000042118
rattus_norvegicus	Bhmt2	ENSRNOG00000040120

Paralogs (4): MTR (ENSG00000116984), UROD (ENSG00000126088), BHMT (ENSG00000145692), MTHFR (ENSG00000177000)

Protein

Protein identifiers

S-methylmethionine–homocysteine S-methyltransferase BHMT2 — Q9H2M3 (reviewed: Q9H2M3)

Alternative names: Betaine–homocysteine S-methyltransferase 2

All UniProt accessions (3): E5RGH5, E5RH96, Q9H2M3

UniProt curated annotations — full annotation on UniProt →

Function. Involved in the regulation of homocysteine metabolism. Converts homocysteine to methionine using S-methylmethionine (SMM) as a methyl donor.

Subunit / interactions. Homotetramer. May interact with PRNP.

Tissue specificity. Expressed in liver and kidney and at reduced levels in the brain, heart, and skeletal muscle.

Cofactor. Binds 1 zinc ion per subunit.

Pathway. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.

Isoforms (2)

UniProt ID	Names	Canonical?
Q9H2M3-1	1	yes
Q9H2M3-2	2

RefSeq proteins (2): NP_001171476, NP_060084* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR003726	HCY_dom	Domain
IPR017226	BHMT-like	Family
IPR036589	HCY_dom_sf	Homologous_superfamily
IPR051524	BHMT	Family

Pfam: PF02574

Catalyzed reactions (Rhea), 1 shown:

• S-methyl-L-methionine + L-homocysteine = 2 L-methionine + H(+) (RHEA:26337)

UniProt features (7 total): binding site 3, chain 1, domain 1, modified residue 1, splice variant 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9H2M3-F1	95.82	0.92

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (3): 208; 290; 291

Post-translational modifications (1): 321

Function

Pathways and Gene Ontology

Reactome pathways

3 pathways

ID	Pathway
R-HSA-1614635	Sulfur amino acid metabolism
R-HSA-1430728	Metabolism
R-HSA-71291	Metabolism of amino acids and derivatives

MSigDB gene sets: 106 (showing top): GOBP_ALPHA_AMINO_ACID_METABOLIC_PROCESS, GOBP_AMINO_ACID_BIOSYNTHETIC_PROCESS, GOBP_ASPARTATE_FAMILY_AMINO_ACID_BIOSYNTHETIC_PROCESS, GOBP_ORGANIC_ACID_BIOSYNTHETIC_PROCESS, GOBP_SMALL_MOLECULE_BIOSYNTHETIC_PROCESS, GOBP_SULFUR_COMPOUND_BIOSYNTHETIC_PROCESS, GOBP_S_ADENOSYLMETHIONINE_METABOLIC_PROCESS, SHETH_LIVER_CANCER_VS_TXNIP_LOSS_PAM4, BLALOCK_ALZHEIMERS_DISEASE_UP, GOBP_METHIONINE_BIOSYNTHETIC_PROCESS, GOBP_METHIONINE_METABOLIC_PROCESS, GOBP_ORGANIC_ACID_METABOLIC_PROCESS, GOBP_AMINO_ACID_BETAINE_METABOLIC_PROCESS, GOBP_SULFUR_AMINO_ACID_METABOLIC_PROCESS, GOBP_METHYLATION

GO Biological Process (6): methylation (GO:0032259), S-methylmethionine metabolic process (GO:0033477), S-adenosylmethionine metabolic process (GO:0046500), L-methionine salvage (GO:0071267), modified amino acid metabolic process (GO:0006575), obsolete methionine biosynthetic process (GO:0009086)

GO Molecular Function (6): zinc ion binding (GO:0008270), S-methylmethionine-homocysteine S-methyltransferase activity (GO:0061627), methyltransferase activity (GO:0008168), S-methyltransferase activity (GO:0008172), transferase activity (GO:0016740), metal ion binding (GO:0046872)

GO Cellular Component (2): cytosol (GO:0005829), extracellular exosome (GO:0070062)

Reactome top-level categories

Rollup of top-2 pathways:

Category	Pathways
Metabolism of amino acids and derivatives	1
Metabolism	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
metabolic process	2
sulfur compound metabolic process	2
modified amino acid metabolic process	1
amino acid salvage	1
L-methionine biosynthetic process	1
transition metal ion binding	1
S-methyltransferase activity	1
transferase activity, transferring one-carbon groups	1
methyltransferase activity	1
catalytic activity	1
cation binding	1
cytoplasm	1
cellular anatomical structure	1
extracellular vesicle	1

Protein interactions and networks

STRING

1274 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
BHMT2	DMGDH	Q9UI17	814
BHMT2	MTRR	Q9UBK8	795
BHMT2	MTHFR	P42898	672
BHMT2	AHCY	P23526	661
BHMT2	P0DN79	P0DN79	659
BHMT2	H7C2H4	H7C2H4	658
BHMT2	MAT1A	Q00266	636
BHMT2	SHMT1	P34896	625
BHMT2	MTHFD1	P11586	622
BHMT2	AHCYL2	Q96HN2	559
BHMT2	GNMT	Q14749	542
BHMT2	CTH	P32929	534
BHMT2	TCN2	P20062	527
BHMT2	MTR	Q99707	525
BHMT2	ENOPH1	Q9UHY7	524

IntAct

6 interactions, top by confidence:

A	B	Type	Score
BHMT2	INPPL1	psi-mi:“MI:0914”(association)	0.530
BHMT	BHMT2	psi-mi:“MI:0914”(association)	0.530
BHMT2	GAPDHS	psi-mi:“MI:0915”(physical association)	0.400

BioGRID (13): BHMT (Affinity Capture-MS), INPPL1 (Affinity Capture-MS), TBCE (Affinity Capture-MS), BHMT2 (Affinity Capture-MS), TBCE (Affinity Capture-MS), INPPL1 (Affinity Capture-MS), BHMT2 (Affinity Capture-MS), INPPL1 (Affinity Capture-MS), BHMT2 (Affinity Capture-MS), BIRC7 (Affinity Capture-MS), TBCE (Affinity Capture-MS), GAPDHS (Affinity Capture-MS), APP (Reconstituted Complex)

ESM2 similar proteins: A0A1S4BZI5, A7M6E7, A7M6E8, B4G0F3, B8BKI7, B9SQI7, C6JS30, F4JGR5, F4JP46, F4JVN6, O04015, O04226, O65361, O80574, O95394, P11172, P31754, P32296, P69060, Q10P67, Q10S55, Q28EN2, Q2R483, Q32LQ4, Q3SZM5, Q53JY8, Q5R514, Q5R6R5, Q5RAK7, Q5RF32, Q5XGM3, Q6P6M7, Q6STH5, Q8GSJ1, Q8LAX0, Q8LB01, Q8NFW8, Q90WG6, Q91WS4, Q94A08

Diamond homologs: O06745, O09171, O33259, O33465, P13009, P37586, Q49775, Q55786, Q5M8Z0, Q5RF32, Q95332, Q9AJQ8, Q9H2M3, O35490, Q32LQ4, Q5I597, Q5RFG2, Q5XGM3, Q68FT5, Q91WS4, Q93088

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

69 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	59
Likely benign	3
Benign	1

Top pathogenic / likely-pathogenic (0)

SpliceAI

1120 predictions. Top by Δscore:

Variant	Effect	Δscore
5:79069813:AAGGT:A	donor_loss	1.0000
5:79069814:AGGTG:A	donor_loss	1.0000
5:79069815:GGTG:G	donor_loss	1.0000
5:79069816:GTG:G	donor_loss	1.0000
5:79079342:A:AG	acceptor_gain	1.0000
5:79083188:ACAG:A	acceptor_gain	1.0000
5:79083188:ACAGG:A	acceptor_gain	1.0000
5:79069812:GAAG:G	donor_gain	0.9900
5:79069817:T:G	donor_loss	0.9900
5:79079342:AAT:A	acceptor_gain	0.9900
5:79079343:A:G	acceptor_gain	0.9900
5:79079344:T:G	acceptor_gain	0.9900
5:79079349:A:AG	acceptor_gain	0.9900
5:79079350:A:G	acceptor_gain	0.9900
5:79083186:GCACA:G	acceptor_loss	0.9900
5:79083187:CACAG:C	acceptor_loss	0.9900
5:79083189:C:G	acceptor_gain	0.9900
5:79083189:CA:C	acceptor_loss	0.9900
5:79083190:A:AC	acceptor_loss	0.9900
5:79083190:A:AG	acceptor_gain	0.9900
5:79083190:AG:A	acceptor_gain	0.9900
5:79083190:AGG:A	acceptor_gain	0.9900
5:79083190:AGGG:A	acceptor_gain	0.9900
5:79083191:G:GG	acceptor_gain	0.9900
5:79083191:GG:G	acceptor_gain	0.9900
5:79083191:GGG:G	acceptor_gain	0.9900
5:79083191:GGGG:G	acceptor_gain	0.9900
5:79083263:G:GT	donor_gain	0.9900
5:79083266:GGTCT:G	donor_gain	0.9900
5:79069816:G:GG	donor_gain	0.9800

AlphaMissense

2378 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
5:79077528:A:C	S28R	0.985
5:79077530:C:A	S28R	0.985
5:79077530:C:G	S28R	0.985
5:79079369:T:A	V56D	0.978
5:79079428:T:C	F76L	0.974
5:79079430:T:A	F76L	0.974
5:79079430:T:G	F76L	0.974
5:79079398:G:C	A66P	0.967
5:79079411:T:A	V70D	0.966
5:79079389:T:C	F63L	0.965
5:79079391:C:A	F63L	0.965
5:79079391:C:G	F63L	0.965
5:79080709:C:A	A94D	0.965
5:79083712:G:A	G289E	0.961
5:79083743:G:C	R299S	0.961
5:79083743:G:T	R299S	0.961
5:79083744:G:C	A300P	0.961
5:79080757:C:A	A110E	0.960
5:79079378:T:C	L59P	0.959
5:79083214:C:A	N207K	0.958
5:79083214:C:G	N207K	0.958
5:79077520:G:A	G25E	0.955
5:79083236:A:C	S215R	0.955
5:79083238:C:A	S215R	0.955
5:79083238:C:G	S215R	0.955
5:79083653:A:C	R269S	0.955
5:79083653:A:T	R269S	0.955
5:79088511:G:C	W343C	0.955
5:79088511:G:T	W343C	0.955
5:79083760:T:C	L305P	0.954

dbSNP variants (sampled 300 via entrez): RS1000004663 (5:79071837 A>C,T), RS1000092160 (5:79076776 C>T), RS1000144343 (5:79076453 G>C,T), RS1000241362 (5:79071343 A>G), RS1000287640 (5:79084025 A>G), RS1000303490 (5:79083492 T>G), RS1000581075 (5:79082060 G>A), RS1000888109 (5:79082563 G>A), RS1001057054 (5:79076048 G>A), RS1001657569 (5:79087730 G>A), RS1001757983 (5:79075092 G>A), RS1001810317 (5:79074729 C>A,T), RS1002090090 (5:79073533 G>A), RS1002141602 (5:79087943 G>C), RS1002270042 (5:79068974 T>C)

Disease associations

OMIM: gene MIM:605932 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

17 associations (top):

Study	Trait	p-value
GCST001852_5	Metabolite levels	2.000000e-09
GCST002039_10	Blood trace element (Se levels)	8.000000e-12
GCST002039_2	Blood trace element (Se levels)	9.000000e-28
GCST002670_1	Blood and toenail selenium levels	2.000000e-39
GCST002670_10	Blood and toenail selenium levels	8.000000e-20
GCST002670_2	Blood and toenail selenium levels	6.000000e-11
GCST002670_7	Blood and toenail selenium levels	2.000000e-15
GCST002670_8	Blood and toenail selenium levels	2.000000e-11
GCST002670_9	Blood and toenail selenium levels	1.000000e-36
GCST002671_15	Toenail selenium levels	3.000000e-13
GCST002671_16	Toenail selenium levels	5.000000e-06
GCST002671_5	Toenail selenium levels	1.000000e-16
GCST002671_6	Toenail selenium levels	2.000000e-11
GCST003358_1	Betaine levels in individuals undergoing cardiac evaluation	7.000000e-13
GCST006997_1	Cerebrospinal fluid t-tau levels in mild cognitive impairment	2.000000e-07
GCST009391_144	Metabolite levels	7.000000e-33
GCST012020_108	Serum metabolite levels	2.000000e-19

EFO canonical traits (4, from GWAS)

EFO ID	Trait name
EFO:0004471	insulin sensitivity measurement
EFO:0007787	plasma betaine measurement
EFO:0004760	t-tau measurement
EFO:0010476	dimethylglycine measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (2): CHEMBL2163167 (SINGLE PROTEIN), CHEMBL3391661 (PROTEIN FAMILY)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
7.11	Ki	77	nM	CHEMBL2164724
5.44	IC50	3600	nM	CHEMBL2164724

PubChem BioAssay actives

2 with measured affinity, of 11 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
2-amino-7-[(3S)-3-amino-3-carboxypropyl]sulfanyl-5-methylheptanoic acid	697235: Competitive inhibition of recombinant human BHMT2 expressed in Escherichia coli BL31(DE3) by Dixon plot method in presence of D,L-homocysteine	ki	0.0770	uM

CTD chemical–gene interactions

22 total (human), top 22 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Benzo(a)pyrene	decreases expression, decreases methylation, increases expression, increases methylation	5
sodium arsenite	increases expression	2
Acetaminophen	decreases expression, increases expression	2
Silicon Dioxide	decreases expression	2
Cyclosporine	increases expression, decreases expression	2
Aflatoxin B1	affects expression, decreases methylation	2
testosterone enanthate	affects expression	1
pirinixic acid	affects binding, increases activity, increases expression	1
bisphenol A	increases expression	1
ethyl-p-hydroxybenzoate	increases expression	1
bisphenol S	affects cotreatment, decreases expression	1
Dexamethasone	affects cotreatment, decreases expression	1
Diethylhexyl Phthalate	decreases expression	1
Indomethacin	decreases expression, affects cotreatment	1
Methionine	decreases reaction, increases metabolic processing	1
Quercetin	decreases expression	1
S-Adenosylmethionine	decreases reaction, increases metabolic processing	1
Valproic Acid	decreases expression	1
Vitamin U	increases metabolic processing, decreases reaction	1
1-Methyl-3-isobutylxanthine	affects cotreatment, decreases expression	1
Okadaic Acid	decreases expression	1
Copper Sulfate	increases expression	1

ChEMBL screening assays

3 unique, capped per target: 3 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL2166486	Binding	Inhibition of recombinant human BHMT2 expressed in Escherichia coli BL31(DE3) at 20 uM	Double-headed sulfur-linked amino acids as first inhibitors for betaine-homocysteine S-methyltransferase 2. — J Med Chem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.