BROX

Summary

BROX (BRO1 domain and CAAX motif containing, HGNC:26512) is a protein-coding gene on chromosome 1q41, encoding BRO1 domain-containing protein BROX (Q5VW32). Nuclear envelope-associated factor that is involved in the nuclear envelope ruptures during interphase (NERDI) repair, where it is locally recruited by CHMP5 and reduces cytoskeletal stress through its action on SYN2 to help reseal the ruptured membrane.

Involved in mitotic nuclear membrane reassembly. Located in nuclear envelope.

Source: NCBI Gene 148362 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 56 total
• Druggable target: yes
• MANE Select transcript: NM_144695

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:26512
Approved symbol	BROX
Name	BRO1 domain and CAAX motif containing
Location	1q41
Locus type	gene with protein product
Status	Approved
Aliases	FLJ32421
Ensembl gene	ENSG00000162819
Ensembl biotype	protein_coding
Entrez	148362

Gene structure

Transcript identifiers

Ensembl transcripts: 25 — 21 protein_coding, 4 protein_coding_CDS_not_defined

ENST00000340934, ENST00000426638, ENST00000469236, ENST00000473962, ENST00000489191, ENST00000496267, ENST00000537020, ENST00000539697, ENST00000612948, ENST00000888412, ENST00000888413, ENST00000916810, ENST00000916814, ENST00000916816, ENST00000916817, ENST00000916818, ENST00000962607, ENST00000962608, ENST00000962609, ENST00000962610, ENST00000962611, ENST00000962612, ENST00000962613, ENST00000962614, ENST00000962615

RefSeq mRNA: 6 — MANE Select: NM_144695 NM_001288579, NM_001288580, NM_001288581, NM_001375661, NM_001375758, NM_144695

CCDS: CCDS1534, CCDS73036, CCDS73037

Canonical transcript exons

ENST00000340934 — 13 exons

Exon	Start	End
ENSE00001068953	222722419	222722514
ENSE00001068955	222728743	222728828
ENSE00001068957	222729620	222729701
ENSE00001068958	222727168	222727257
ENSE00001068962	222731357	222731516
ENSE00001068963	222730027	222730177
ENSE00003598268	222725450	222725555
ENSE00003600801	222718925	222719031
ENSE00003612560	222724092	222724164
ENSE00003628196	222715684	222715800
ENSE00003674541	222719263	222719359
ENSE00003841794	222732628	222735187
ENSE00003845726	222712564	222712942

Expression profiles

Bgee: expression breadth ubiquitous, 254 present calls, max score 98.46.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 32.7380 / max 215.3625, expressed in 1818 samples.

FANTOM5 promoters (6 alternative TSS)

Promoter ID	TPM avg	Samples expressed
8724	10.0930	1762
8726	8.2018	1716
8728	7.7353	1704
8727	3.4170	1206
8725	3.1736	1431
8729	0.1172	49

Top tissues by expression

256 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
ileal mucosa	UBERON:0000331	98.46	gold quality
cardiac muscle of right atrium	UBERON:0003379	97.79	gold quality
palpebral conjunctiva	UBERON:0001812	97.73	gold quality
nasal cavity epithelium	UBERON:0005384	97.32	gold quality
upper arm skin	UBERON:0004263	97.24	gold quality
corpus callosum	UBERON:0002336	97.16	gold quality
calcaneal tendon	UBERON:0003701	96.89	gold quality
jejunal mucosa	UBERON:0000399	96.82	gold quality
pons	UBERON:0000988	96.80	gold quality
trigeminal ganglion	UBERON:0001675	96.69	gold quality
mucosa of sigmoid colon	UBERON:0004993	96.65	gold quality
corpus epididymis	UBERON:0004359	96.56	gold quality
left ventricle myocardium	UBERON:0006566	96.53	gold quality
kidney epithelium	UBERON:0004819	96.45	gold quality
mucosa of paranasal sinus	UBERON:0005030	96.40	gold quality
esophagus squamous epithelium	UBERON:0006920	96.40	gold quality
colonic mucosa	UBERON:0000317	96.21	gold quality
tibialis anterior	UBERON:0001385	96.09	gold quality
deltoid	UBERON:0001476	96.06	gold quality
oral cavity	UBERON:0000167	96.03	gold quality
epithelium of nasopharynx	UBERON:0001951	95.94	gold quality
penis	UBERON:0000989	95.93	gold quality
bronchial epithelial cell	CL:0002328	95.84	gold quality
subthalamic nucleus	UBERON:0001906	95.83	gold quality
bronchus	UBERON:0002185	95.71	gold quality
caput epididymis	UBERON:0004358	95.61	gold quality
pharyngeal mucosa	UBERON:0000355	95.55	gold quality
lateral globus pallidus	UBERON:0002476	95.54	gold quality
inferior vagus X ganglion	UBERON:0005363	95.50	gold quality
medulla oblongata	UBERON:0001896	95.46	gold quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-MTAB-6058	no	1549.53
E-MTAB-4850	no	412.24
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

148 targeting BROX, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-5011-5P	100.00	83.46	5820
HSA-MIR-3613-3P	100.00	76.36	7965
HSA-MIR-190A-3P	100.00	80.35	5520
HSA-MIR-4476	100.00	68.18	2030
HSA-MIR-6876-5P	100.00	67.68	2126
HSA-MIR-4533	100.00	69.48	2758
HSA-MIR-3163	100.00	77.23	8605
HSA-MIR-548C-3P	99.99	74.01	7587
HSA-MIR-4531	99.99	69.70	3181
HSA-MIR-4282	99.99	75.36	6408
HSA-MIR-4789-5P	99.98	70.76	2721
HSA-MIR-12136	99.98	72.81	5713
HSA-MIR-3065-5P	99.97	71.56	3281
HSA-MIR-607	99.97	73.62	5593
HSA-MIR-5688	99.96	73.23	4504
HSA-MIR-495-3P	99.96	72.81	4197
HSA-MIR-548AJ-3P	99.96	73.38	5345
HSA-MIR-548X-3P	99.96	73.38	5345
HSA-MIR-1250-3P	99.96	70.04	4038
HSA-MIR-570-3P	99.96	72.41	4910
HSA-MIR-3658	99.96	73.87	4379
HSA-MIR-548AB	99.95	71.31	3488
HSA-MIR-559	99.95	72.28	3609
HSA-MIR-548J-3P	99.94	72.61	4881
HSA-MIR-548A-5P	99.94	71.27	3482
HSA-MIR-548AD-5P	99.94	71.23	3502
HSA-MIR-548AE-5P	99.94	71.23	3502
HSA-MIR-548AK	99.94	71.24	3488
HSA-MIR-548AM-5P	99.94	71.24	3488
HSA-MIR-548AP-5P	99.94	71.14	3489

Literature-anchored findings (GeneRIF, showing 4)

• Results revealed that Strep-Brox(WT) exhibited accumulation in the perinuclear area suggested physiological significance of farnesylation of Brox in its subcellular distribution and efficient interaction with CHMP4s in vivo. (PMID:18190528)
• structural analysis of the Bro1 domain protein BROX and functional analyses of the ALIX Bro1 domain in HIV-1 budding (PMID:22162750)
• homologous domain of human Bro1 domain-containing proteins, Alix and Brox, binds CHMP4B but not STAM2, despite their high structural similarity (PMID:26866605)
• BROX haploinsufficiency in familial nonmedullary thyroid cancer. (PMID:32385852)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	brox	ENSDARG00000013659
mus_musculus	Brox	ENSMUSG00000046836
rattus_norvegicus	Brox	ENSRNOG00000052963
caenorhabditis_elegans		WBGENE00015219

Protein

Protein identifiers

BRO1 domain-containing protein BROX — Q5VW32 (reviewed: Q5VW32)

Alternative names: BRO1 domain- and CAAX motif-containing protein

All UniProt accessions (3): Q5VW32, F5GXQ0, Q5VW33

UniProt curated annotations — full annotation on UniProt →

Function. Nuclear envelope-associated factor that is involved in the nuclear envelope ruptures during interphase (NERDI) repair, where it is locally recruited by CHMP5 and reduces cytoskeletal stress through its action on SYN2 to help reseal the ruptured membrane.

Subunit / interactions. Monomer. Interacts with CHMP4B. Interacts with CHMP5: this interaction allows the recruitment of BROX to cellular membranes. Interacts with SYN2; this interaction promotes SYN2 ubiquitination and facilitates the relaxation of mechanical stress imposed by compressive actin fibers at the rupture site.

Subcellular location. Nucleus membrane.

Post-translational modifications. Farnesylation is required for nuclear envelope localization.

Similarity. Belongs to the BROX family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q5VW32-1	1	yes
Q5VW32-2	2

RefSeq proteins (6): NP_001275508, NP_001275509, NP_001275510, NP_001362590, NP_001362687, NP_653296* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR004328	BRO1_dom	Domain
IPR038499	BRO1_sf	Homologous_superfamily
IPR038898	BROX	Family

Pfam: PF03097

UniProt features (36 total): helix 15, strand 7, mutagenesis site 3, modified residue 2, chain 1, propeptide 1, sequence conflict 1, turn 1, domain 1, region of interest 1, compositionally biased region 1, lipid moiety-binding region 1, splice variant 1

Structure

Experimental structures (PDB)

7 structures.

PDB	Method	Resolution (Å)
3R9M	X-RAY DIFFRACTION	1.95
3ZXP	X-RAY DIFFRACTION	2.5
3UM2	X-RAY DIFFRACTION	2.59
3ULY	X-RAY DIFFRACTION	2.6
3UM1	X-RAY DIFFRACTION	2.71
3UM0	X-RAY DIFFRACTION	3.1
3UM3	X-RAY DIFFRACTION	3.8

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q5VW32-F1	92.15	0.84

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (3): 283, 408, 408

Mutagenesis-validated functional residues (3):

Position	Phenotype
350	loss of syn2 interaction. abolishes brox recruitment to sites of nuclear envelope (ne) rupture. impairs ne resealing. do
408	loss of association with the nuclear envelop. abolishes its recruitment to rupture sites. abolishes its repair function.
204	does not affect syn2 interaction. does not induce syn2 ubiquitination. does not affect recruitment to sites of rupture.

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 92 (showing top): GOBP_NUCLEAR_MEMBRANE_REASSEMBLY, GOBP_MEMBRANE_BIOGENESIS, MAYBURD_RESPONSE_TO_L663536_UP, GOBP_ORGANELLE_FISSION, GOBP_NUCLEUS_ORGANIZATION, GOBP_MITOTIC_NUCLEAR_DIVISION, GOBP_ENDOMEMBRANE_SYSTEM_ORGANIZATION, GOBP_MITOTIC_CELL_CYCLE, GOBP_NUCLEAR_ENVELOPE_ORGANIZATION, GOBP_MEMBRANE_ORGANIZATION, OSMAN_BLADDER_CANCER_DN, GOCC_NUCLEAR_ENVELOPE, GOCC_NUCLEAR_MEMBRANE, GOBP_CELL_CYCLE_PROCESS, GOCC_ORGANELLE_ENVELOPE

GO Biological Process (1): mitotic nuclear membrane reassembly (GO:0007084)

GO Molecular Function (1): protein binding (GO:0005515)

GO Cellular Component (5): nuclear envelope (GO:0005635), nuclear membrane (GO:0031965), extracellular exosome (GO:0070062), nucleus (GO:0005634), membrane (GO:0016020)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
nucleus	2
mitotic cell cycle	1
nuclear membrane reassembly	1
mitotic nuclear membrane organization	1
binding	1
endomembrane system	1
organelle envelope	1
nuclear envelope	1
organelle membrane	1
extracellular vesicle	1
intracellular membrane-bounded organelle	1
cellular anatomical structure	1

Protein interactions and networks

STRING

843 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
BROX	CHMP6	Q96FZ7	590
BROX	CHMP4A	Q9BY43	540
BROX	CHMP5	Q9NZZ3	512
BROX	VPS28	Q9UK41	507
BROX	STAM2	O75886	494
BROX	VPS25	Q9BRG1	480
BROX	CHMP3	Q9Y3E7	466
BROX	A0A140T963	A0A140T963	466
BROX	VTA1	Q9NP79	462
BROX	RHBDD2	Q6NTF9	459
BROX	ANO7	Q6IWH7	459
BROX	SNF8	Q96H20	459
BROX	TSG101	Q99816	458
BROX	HABP2	Q14520	450
BROX	CHMP1A	Q9HD42	446

IntAct

63 interactions, top by confidence:

A	B	Type	Score
BROX	CHMP5	psi-mi:“MI:0407”(direct interaction)	0.610
BROX	CHMP5	psi-mi:“MI:0915”(physical association)	0.610
BROX	CHMP4B	psi-mi:“MI:0407”(direct interaction)	0.610
BROX	CHMP4B	psi-mi:“MI:0915”(physical association)	0.610
BROX	TCEA1	psi-mi:“MI:0914”(association)	0.560
BROX	TCEA1	psi-mi:“MI:0915”(physical association)	0.560
BROX	CHMP4A	psi-mi:“MI:0915”(physical association)	0.400
BROX	CHMP4C	psi-mi:“MI:0915”(physical association)	0.400
BROX	HNRNPCL2	psi-mi:“MI:0915”(physical association)	0.400
	TK2	psi-mi:“MI:0915”(physical association)	0.400
BROX		psi-mi:“MI:0915”(physical association)	0.370
NS3	C15orf61	psi-mi:“MI:0914”(association)	0.350
	SHTN1	psi-mi:“MI:0914”(association)	0.350
	TNFRSF10A	psi-mi:“MI:0914”(association)	0.350
RNF149	CCDC85C	psi-mi:“MI:0914”(association)	0.350
NFYA	NME2P1	psi-mi:“MI:0914”(association)	0.350
ZDHHC6	A2ML1	psi-mi:“MI:0914”(association)	0.350
AIMP1	IGF2BP3	psi-mi:“MI:0914”(association)	0.350
AIMP1	TRAFD1	psi-mi:“MI:0914”(association)	0.350
MAVS	CHMP2A	psi-mi:“MI:0914”(association)	0.350
CACYBP	VPS37C	psi-mi:“MI:0914”(association)	0.350
CACYBP	PSMD11	psi-mi:“MI:0914”(association)	0.350

BioGRID (52): BROX (Co-fractionation), LDHA (Co-fractionation), PDCD6IP (Co-fractionation), SORD (Co-fractionation), TCEA1 (Affinity Capture-MS), CHMP1A (Affinity Capture-MS), IPO9 (Affinity Capture-MS), BROX (Affinity Capture-MS), BROX (Affinity Capture-MS), BROX (Affinity Capture-MS), BROX (Proximity Label-MS), BROX (Affinity Capture-MS), BROX (Affinity Capture-MS), BROX (Affinity Capture-MS), TCEA1 (Affinity Capture-MS)

ESM2 similar proteins: A3KQV2, A4IIL4, O00471, O54921, P48553, P62944, P63009, P63010, P79020, P97878, Q00004, Q0IIZ5, Q1L8F9, Q29RF7, Q3SYS9, Q3TLI0, Q3TPX4, Q498H0, Q4G074, Q4QR29, Q4V8K5, Q4VA53, Q5F3K0, Q5KU05, Q5R6J0, Q5RDD7, Q5VW32, Q5VYK3, Q62018, Q6DDM4, Q6DEU9, Q6IR80, Q6P1I3, Q6PD62, Q6PDI5, Q6TRW4, Q6ZPU9, Q8BMA6, Q8K2Q7, Q8WUM4

Diamond homologs: A3KQV2, A4IIL4, Q22885, Q4V8K5, Q5RDD7, Q5VW32, Q8K2Q7, Q756C5, Q12033, Q6CU63, Q8WZL4

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 59 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Budding and maturation of HIV virion	6	61.2×	1e-07
Endosomal Sorting Complex Required For Transport (ESCRT)	6	55.3×	1e-07
Sealing of the nuclear envelope (NE) by ESCRT-III	5	43.3×	6e-06
Late endosomal microautophagy	5	40.8×	6e-06
HCMV Late Events	5	12.3×	2e-03

GO biological processes:

GO term	Partners	Fold	FDR
nuclear membrane reassembly	6	110.1×	4e-09
late endosome to lysosome transport	5	91.8×	7e-08
viral budding via host ESCRT complex	6	89.2×	6e-09
multivesicular body sorting pathway	5	74.3×	2e-07
midbody abscission	5	67.8×	3e-07
regulation of mitotic spindle assembly	5	67.8×	3e-07
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	6	60.4×	3e-08
multivesicular body assembly	6	58.5×	3e-08

Disease & clinical

Clinical variants and AI predictions

ClinVar

56 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	39
Likely benign	1
Benign	2

Top pathogenic / likely-pathogenic (0)

SpliceAI

1980 predictions. Top by Δscore:

Variant	Effect	Δscore
1:222713668:G:GG	donor_gain	1.0000
1:222713703:GGAA:G	donor_gain	1.0000
1:222713704:G:GT	donor_gain	1.0000
1:222713726:G:GT	donor_gain	1.0000
1:222713726:GAA:G	donor_gain	1.0000
1:222713760:C:G	donor_gain	1.0000
1:222715682:A:AG	acceptor_gain	1.0000
1:222715683:G:GG	acceptor_gain	1.0000
1:222715797:GCAA:G	donor_gain	1.0000
1:222715799:AAGT:A	donor_loss	1.0000
1:222715800:AG:A	donor_loss	1.0000
1:222715801:G:GG	donor_gain	1.0000
1:222715801:GTA:G	donor_loss	1.0000
1:222715802:T:A	donor_loss	1.0000
1:222718921:T:G	acceptor_gain	1.0000
1:222718922:A:AG	acceptor_gain	1.0000
1:222718922:AAGT:A	acceptor_gain	1.0000
1:222718923:A:AG	acceptor_gain	1.0000
1:222718924:G:GG	acceptor_gain	1.0000
1:222718924:GT:G	acceptor_gain	1.0000
1:222719258:TATA:T	acceptor_loss	1.0000
1:222719260:TA:T	acceptor_loss	1.0000
1:222719261:A:AC	acceptor_loss	1.0000
1:222719261:A:AG	acceptor_gain	1.0000
1:222719262:G:GA	acceptor_gain	1.0000
1:222719353:TTCCA:T	donor_gain	1.0000
1:222719356:CAAGG:C	donor_loss	1.0000
1:222719357:AAGG:A	donor_loss	1.0000
1:222719358:AGGTA:A	donor_loss	1.0000
1:222719359:GGTAA:G	donor_loss	1.0000

AlphaMissense

2678 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
1:222719328:T:A	W92R	1.000
1:222719328:T:C	W92R	1.000
1:222728784:T:A	W238R	1.000
1:222728784:T:C	W238R	1.000
1:222715709:T:A	W4R	0.999
1:222715709:T:C	W4R	0.999
1:222715719:G:T	R7M	0.999
1:222715732:A:C	K11N	0.999
1:222715732:A:T	K11N	0.999
1:222724130:T:C	L147P	0.999
1:222724139:C:A	A150E	0.999
1:222724141:G:C	A151P	0.999
1:222725540:G:C	A189P	0.999
1:222725541:C:A	A189D	0.999
1:222725546:G:C	A191P	0.999
1:222725547:C:A	A191D	0.999
1:222727180:G:C	R198P	0.999
1:222727182:G:C	A199P	0.999
1:222727183:C:A	A199E	0.999
1:222727216:C:A	A210D	0.999
1:222727224:G:C	A213P	0.999
1:222729636:G:A	G258D	0.999
1:222729671:G:C	A270P	0.999
1:222729684:T:C	L274P	0.999
1:222729692:G:C	A277P	0.999
1:222730169:T:A	N327K	0.999
1:222730169:T:G	N327K	0.999
1:222730177:T:A	I330N	0.999
1:222730177:T:C	I330T	0.999
1:222731358:T:G	Y331D	0.999

dbSNP variants (sampled 300 via entrez): RS1000095755 (1:222711862 G>A), RS1000323571 (1:222715151 A>C), RS1000553936 (1:222728168 C>T), RS1000718400 (1:222715536 C>T), RS1000882652 (1:222735329 A>G), RS1000915108 (1:222715869 A>G,T), RS1001127741 (1:222731605 A>G), RS1001139450 (1:222727768 A>G,T), RS1001388798 (1:222718374 G>A,T), RS1001450338 (1:222725045 G>A,C), RS1001524508 (1:222712830 C>T), RS1001587791 (1:222718574 T>A), RS1001710497 (1:222734148 T>C), RS1001732869 (1:222715958 C>A,T), RS1001763014 (1:222733725 G>T)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6066473 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

PharmGKB variants

2 variants.

Variant	Genes	Level	Score	#Clin annots	Drugs
rs3748631	AIDA, BROX		0.00	0
rs35762933	AIDA, BROX		0.00	0

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
7.31	Kd	48.97	nM	CHEMBL5653589
7.31	ED50	48.97	nM	CHEMBL5653589

PubChem BioAssay actives

1 with measured affinity, of 2 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2147959: Binding affinity to human BROX incubated for 45 mins by Kinobead based pull down assay	kd	0.0490	uM

CTD chemical–gene interactions

35 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Tobacco Smoke Pollution	affects expression, increases expression	2
GSK-J4	increases expression	1
bisphenol F	affects cotreatment, increases expression	1
triphenyl phosphate	affects expression	1
alpha-pinene	increases oxidation, increases abundance, affects cotreatment	1
bisphenol A	affects cotreatment, increases expression	1
tris(1,3-dichloro-2-propyl)phosphate	increases expression	1
sodium arsenite	increases expression	1
tetrabromobisphenol A	decreases expression	1
resorcinol	decreases expression	1
methacrylaldehyde	affects cotreatment, increases oxidation, increases abundance	1
2,2’,4,4’-tetrabromodiphenyl ether	decreases expression	1
hexabrominated diphenyl ether 153	decreases expression	1
bisphenol S	affects cotreatment, increases expression	1
jinfukang	decreases expression	1
Acrolein	affects cotreatment, increases oxidation, increases abundance	1
Air Pollutants	affects cotreatment, increases abundance, increases oxidation	1
Benzo(a)pyrene	affects methylation	1
Dexamethasone	affects cotreatment, increases expression	1
Doxorubicin	decreases expression	1
Ethyl Methanesulfonate	increases expression	1
Formaldehyde	increases expression	1
Indomethacin	affects cotreatment, increases expression	1
Ivermectin	decreases expression	1
Methyl Methanesulfonate	increases expression	1
Ozone	affects cotreatment, increases oxidation, increases abundance	1
Phthalic Acids	increases methylation	1
Tretinoin	increases expression	1
Urethane	increases expression	1
Valproic Acid	increases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5651001	Binding	Binding affinity to human BROX incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.