BYSL

Summary

BYSL (bystin like, HGNC:1157) is a protein-coding gene on chromosome 6p21.1, encoding Bystin (Q13895). Required for processing of 20S pre-rRNA precursor and biogenesis of 40S ribosomal subunits. It is a common-essential gene (DepMap: required in 99.8% of cancer cell lines).

Bystin is expressed as a 2-kb major transcript and a 3.6-kb minor transcript in SNG-M cells and in human trophoblastic teratocarcinoma HT-H cells. Protein binding assays determined that bystin binds directly to trophinin and tastin, and that binding is enhanced when cytokeratins 8 and 18 are present. Immunocytochemistry of HT-H cells showed that bystin colocalizes with trophinin, tastin, and the cytokeratins, suggesting that these molecules form a complex in trophectoderm cells at the time of implantation. Using immunohistochemistry it was determined that trophinin and bystin are found in the placenta from the sixth week of pregnancy. Both proteins were localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10, the levels of trophinin, tastin, and bystin decreased and then disappeared from placental villi.

Source: NCBI Gene 705 — RefSeq curated summary.

At a glance

• GWAS associations: 11
• Clinical variants (ClinVar): 108 total
• Druggable target: yes
• Cancer dependency (DepMap): dependent in 99.8% of screened cell lines (common-essential)
• MANE Select transcript: NM_004053

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:1157
Approved symbol	BYSL
Name	bystin like
Location	6p21.1
Locus type	gene with protein product
Status	Approved
Aliases	Enp1
Ensembl gene	ENSG00000112578
Ensembl biotype	protein_coding
OMIM	603871
Entrez	705

Gene structure

Transcript identifiers

Ensembl transcripts: 10 — 6 protein_coding, 2 nonsense_mediated_decay, 2 retained_intron

ENST00000230340, ENST00000372996, ENST00000475702, ENST00000489290, ENST00000494032, ENST00000715726, ENST00000920331, ENST00000920332, ENST00000920333, ENST00000920334

RefSeq mRNA: 1 — MANE Select: NM_004053 NM_004053

CCDS: CCDS34450

Canonical transcript exons

ENST00000230340 — 7 exons

Exon	Start	End
ENSE00001812166	41930132	41930270
ENSE00003471874	41931396	41931556
ENSE00003596859	41931728	41931830
ENSE00003614422	41927374	41927536
ENSE00003652226	41930635	41930768
ENSE00004027708	41932361	41933046
ENSE00004027709	41921499	41921830

Expression profiles

Bgee: expression breadth ubiquitous, 187 present calls, max score 91.74.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 27.2451 / max 248.2774, expressed in 1803 samples.

FANTOM5 promoters (3 alternative TSS)

Promoter ID	TPM avg	Samples expressed
67779	23.0911	1784
67780	3.7698	1551
67778	0.3842	161

Top tissues by expression

276 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	91.74	gold quality
gastrocnemius	UBERON:0001388	88.41	gold quality
muscle of leg	UBERON:0001383	87.16	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	86.23	gold quality
islet of Langerhans	UBERON:0000006	85.32	gold quality
muscle organ	UBERON:0001630	85.30	gold quality
embryo	UBERON:0000922	84.96	gold quality
ganglionic eminence	UBERON:0004023	84.87	gold quality
right adrenal gland	UBERON:0001233	84.59	gold quality
stromal cell of endometrium	CL:0002255	84.42	gold quality
left adrenal gland	UBERON:0001234	84.29	gold quality
cortical plate	UBERON:0005343	84.22	gold quality
tongue squamous epithelium	UBERON:0006919	84.11	gold quality
right adrenal gland cortex	UBERON:0035827	84.05	gold quality
left adrenal gland cortex	UBERON:0035825	84.03	gold quality
cartilage tissue	UBERON:0002418	84.02	gold quality
hindlimb stylopod muscle	UBERON:0004252	84.00	gold quality
ventricular zone	UBERON:0003053	83.71	gold quality
mucosa of transverse colon	UBERON:0004991	83.17	gold quality
adrenal gland	UBERON:0002369	82.99	gold quality
endometrium epithelium	UBERON:0004811	82.98	silver quality
smooth muscle tissue	UBERON:0001135	82.95	gold quality
adrenal cortex	UBERON:0001235	82.70	gold quality
left uterine tube	UBERON:0001303	82.67	gold quality
esophagus mucosa	UBERON:0002469	82.41	gold quality
apex of heart	UBERON:0002098	82.31	gold quality
triceps brachii	UBERON:0001509	81.95	gold quality
gluteal muscle	UBERON:0002000	81.93	silver quality
lower esophagus mucosa	UBERON:0035834	81.68	gold quality
heart left ventricle	UBERON:0002084	81.52	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	3.49
E-MTAB-3929	no	383.42

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

14 targeting BYSL, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3667-3P	99.99	67.17	1636
HSA-MIR-6077	99.99	68.04	2299
HSA-MIR-9-3P	99.96	70.88	2068
HSA-MIR-95-5P	99.89	72.17	3973
HSA-MIR-4731-5P	99.89	67.23	2537
HSA-MIR-6716-5P	99.56	68.62	1244
HSA-MIR-6733-3P	99.54	67.80	1281
HSA-MIR-6827-5P	98.46	64.88	1256
HSA-MIR-146B-3P	97.83	65.29	782
HSA-MIR-3157-5P	97.41	67.61	998
HSA-MIR-6748-3P	97.20	65.66	836
HSA-MIR-6854-5P	96.77	65.96	848
HSA-MIR-621	96.76	66.89	371
HSA-MIR-4512	95.26	63.08	371

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 99.8% of screened cell lines, common-essential.

Literature-anchored findings (GeneRIF, showing 6)

• These results suggest that, while bystin may play multiple roles in mammalian cells, a conserved function is to facilitate ribosome biogenesis required for cell growth. (PMID:17381424)
• review of the roles of bystin in embryo implantation, assembly of ribosomes, and as a target of c-MYC (PMID:17917702)
• bystin is necessary for efficient cleavage of the internal transcribed spacer 1 at site 2 and for further processing of the pre-rRNA. (PMID:20805244)
• BYSL contributes to tumor growth by cooperating with the mTORC2 complex in gliomas. (PMID:33628587)
• DDX10 and BYSL as the potential targets of chondrosarcoma and glioma. (PMID:34797290)
• Increased Expression and Prognostic Significance of BYSL in Melanoma. (PMID:38980088)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	bysl	ENSDARG00000001057
mus_musculus	Bysl	ENSMUSG00000023988
rattus_norvegicus	Bysl	ENSRNOG00000049121
drosophila_melanogaster	bys	FBGN0010292
caenorhabditis_elegans		WBGENE00000276

Protein

Protein identifiers

Bystin — Q13895 (reviewed: Q13895)

All UniProt accessions (3): Q13895, F8WBL2, H7BY94

UniProt curated annotations — full annotation on UniProt →

Function. Required for processing of 20S pre-rRNA precursor and biogenesis of 40S ribosomal subunits. May be required for trophinin-dependent regulation of cell adhesion during implantation of human embryos.

Subunit / interactions. Binds trophinin, tastin and cytokeratins.

Subcellular location. Cytoplasm. Nucleus. Nucleolus.

Tissue specificity. Found in the placenta from the sixth week of pregnancy. Was localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10, the level decreased and then disappeared from placental villi.

Miscellaneous. HeLa cells lacking BYSL show a delay in the processing of the 18S rRNA component of the 40S ribosomal subunit. HT-H cells lacking BYSL show trophinin-independent signaling through ERBB4.

Similarity. Belongs to the bystin family.

RefSeq proteins (1): NP_004044* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR007955	Bystin	Family

Pfam: PF05291

UniProt features (37 total): helix 21, modified residue 6, sequence variant 2, sequence conflict 2, turn 2, chain 1, region of interest 1, compositionally biased region 1, strand 1

Structure

Experimental structures (PDB)

13 structures.

PDB	Method	Resolution (Å)
7MQA	ELECTRON MICROSCOPY	2.7
7WTU	ELECTRON MICROSCOPY	3
7WTZ	ELECTRON MICROSCOPY	3
7WTT	ELECTRON MICROSCOPY	3.1
7WTX	ELECTRON MICROSCOPY	3.1
7WTW	ELECTRON MICROSCOPY	3.2
7WU0	ELECTRON MICROSCOPY	3.3
7WTV	ELECTRON MICROSCOPY	3.5
6G18	ELECTRON MICROSCOPY	3.6
7MQ8	ELECTRON MICROSCOPY	3.6
7MQ9	ELECTRON MICROSCOPY	3.87
6G4S	ELECTRON MICROSCOPY	4
6G4W	ELECTRON MICROSCOPY	4.5

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q13895-F1	78.24	0.50

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (6): 40, 55, 98, 156, 167, 414

Function

Pathways and Gene Ontology

Reactome pathways

4 pathways

ID	Pathway
R-HSA-6791226	Major pathway of rRNA processing in the nucleolus and cytosol
R-HSA-72312	rRNA processing
R-HSA-8868773	rRNA processing in the nucleus and cytosol
R-HSA-8953854	Metabolism of RNA

MSigDB gene sets: 210 (showing top): GSE45365_NK_CELL_VS_CD11B_DC_DN, GOBP_RIBOSOME_BIOGENESIS, GOBP_EMBRYO_DEVELOPMENT_ENDING_IN_BIRTH_OR_EGG_HATCHING, BASSO_B_LYMPHOCYTE_NETWORK, GOBP_MATURATION_OF_SSU_RRNA, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_DN, GRAESSMANN_RESPONSE_TO_MC_AND_DOXORUBICIN_DN, GOBP_BLASTOCYST_FORMATION, WEI_MYCN_TARGETS_WITH_E_BOX, MUELLER_PLURINET, GOBP_IN_UTERO_EMBRYONIC_DEVELOPMENT, GOBP_RIBOSOMAL_SMALL_SUBUNIT_BIOGENESIS, GOBP_MATURATION_OF_SSU_RRNA_FROM_TRICISTRONIC_RRNA_TRANSCRIPT_SSU_RRNA_5_8S_RRNA_LSU_RRNA, ENGELMANN_CANCER_PROGENITORS_UP, GOBP_BLASTOCYST_DEVELOPMENT

GO Biological Process (8): maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) (GO:0000462), trophectodermal cell differentiation (GO:0001829), rRNA processing (GO:0006364), ribosome biogenesis (GO:0042254), stem cell proliferation (GO:0072089), regulation of protein localization to nucleolus (GO:1904749), in utero embryonic development (GO:0001701), blastocyst formation (GO:0001825)

GO Molecular Function (3): RNA binding (GO:0003723), snoRNA binding (GO:0030515), protein binding (GO:0005515)

GO Cellular Component (9): nucleoplasm (GO:0005654), chromosome (GO:0005694), nucleolus (GO:0005730), cytoplasm (GO:0005737), cytosol (GO:0005829), membrane (GO:0016020), preribosome, small subunit precursor (GO:0030688), apical part of cell (GO:0045177), nucleus (GO:0005634)

Reactome top-level categories

Rollup of top-3 pathways:

Category	Pathways
rRNA processing in the nucleus and cytosol	1
Metabolism of RNA	1
rRNA processing	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	5
nuclear lumen	2
intracellular membraneless organelle	2
maturation of SSU-rRNA	1
blastocyst formation	1
cell differentiation	1
RNA processing	1
rRNA metabolic process	1
ribosome biogenesis	1
ribonucleoprotein complex biogenesis	1
cell population proliferation	1
stem cell division	1
regulation of protein localization to nucleus	1
protein localization to nucleolus	1
chordate embryonic development	1
blastocyst development	1
anatomical structure formation involved in morphogenesis	1
nucleic acid binding	1
RNA binding	1
binding	1
intracellular anatomical structure	1
cytoplasm	1
preribosome	1
intracellular membrane-bounded organelle	1

Protein interactions and networks

STRING

2650 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
BYSL	TRO	Q12816	999
BYSL	TROAP	Q12815	998
BYSL	LTV1	Q96GA3	991
BYSL	TSR1	Q2NL82	963
BYSL	RIOK2	Q9BVS4	942
BYSL	RPS3	P23396	923
BYSL	PNO1	Q9NRX1	905
BYSL	NOB1	Q9ULX3	902
BYSL	RRP12	Q5JTH9	872
BYSL	NOP14	P78316	863
BYSL	RIOK1	Q9BRS2	841
BYSL	DDX49	Q9Y6V7	823
BYSL	PDCD11	Q14690	812
BYSL	NAT10	Q9H0A0	811
BYSL	EMG1	Q92979	806

IntAct

773 interactions, top by confidence:

A	B	Type	Score
BYSL	LTV1	psi-mi:“MI:0915”(physical association)	0.870
LTV1	BYSL	psi-mi:“MI:0915”(physical association)	0.870
BYSL	COIL	psi-mi:“MI:0915”(physical association)	0.840
BYSL	MRFAP1L1	psi-mi:“MI:0915”(physical association)	0.840
MRFAP1L1	BYSL	psi-mi:“MI:0915”(physical association)	0.840
BYSL	TRIM37	psi-mi:“MI:0915”(physical association)	0.780
TRAF4	BYSL	psi-mi:“MI:0915”(physical association)	0.780
BYSL	TRAF4	psi-mi:“MI:0915”(physical association)	0.780
BYSL	PNMA2	psi-mi:“MI:0915”(physical association)	0.780
CCDC102B	BYSL	psi-mi:“MI:0915”(physical association)	0.720
NECAB2	BYSL	psi-mi:“MI:0915”(physical association)	0.720
BYSL	ZBTB14	psi-mi:“MI:0915”(physical association)	0.720
ZBTB14	BYSL	psi-mi:“MI:0915”(physical association)	0.720
STX11	BYSL	psi-mi:“MI:0915”(physical association)	0.720
BYSL	TRIM27	psi-mi:“MI:0915”(physical association)	0.720
BYSL	EMD	psi-mi:“MI:0915”(physical association)	0.720
FXR2	BYSL	psi-mi:“MI:0915”(physical association)	0.720

BioGRID (555): BYSL (Two-hybrid), PHC2 (Two-hybrid), EMD (Two-hybrid), EPS8 (Two-hybrid), GOLGA2 (Two-hybrid), KRT31 (Two-hybrid), MID1 (Two-hybrid), TRIM37 (Two-hybrid), TRIM27 (Two-hybrid), SMN2 (Two-hybrid), TRIP6 (Two-hybrid), UBE2H (Two-hybrid), ZBTB14 (Two-hybrid), AIMP2 (Two-hybrid), COIL (Two-hybrid)

ESM2 similar proteins: A0A097I2D0, A0A1W2PP81, A0A1W2PPE2, A0A1W2PPH5, A0A1W2PPL8, A0A1W2PPW3, A0A1W2PQ09, A0A1W2PR64, A0A1W2PRV1, A6NLC8, F4HR03, O54825, O75461, P0C1H6, P0CV38, P0DMV1, P0DMV2, P0DW11, P0DW12, P0DW13, P0DW14, P40914, P49585, P49906, P81195, Q0MTC0, Q13895, Q15544, Q2N2K6, Q3SZB8, Q5DJT8, Q5RA91, Q5U1X0, Q6CER9, Q6RG77, Q6XL73, Q75DE4, Q7XHR2, Q7Z2G1, Q80WL2

Diamond homologs: A7S7F2, A9UNU6, O54825, O60071, P38333, P51406, Q13895, Q20932, Q54IS0, Q5E9N0, Q80WL2, Q8RWS4

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 59 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Keratinization	5	11.1×	9e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

108 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	88
Likely benign	11
Benign	2

Top pathogenic / likely-pathogenic (0)

SpliceAI

767 predictions. Top by Δscore:

Variant	Effect	Δscore
6:41921829:GG:G	donor_gain	1.0000
6:41921830:GG:G	donor_gain	1.0000
6:41927370:CTA:C	acceptor_loss	1.0000
6:41927371:TAGGT:T	acceptor_loss	1.0000
6:41927372:A:AG	acceptor_gain	1.0000
6:41927372:AGGT:A	acceptor_loss	1.0000
6:41927373:G:GG	acceptor_gain	1.0000
6:41927373:G:GT	acceptor_loss	1.0000
6:41927536:GGT:G	donor_loss	1.0000
6:41927537:G:GA	donor_loss	1.0000
6:41927538:T:G	donor_loss	1.0000
6:41930627:A:AG	acceptor_gain	1.0000
6:41930628:T:G	acceptor_gain	1.0000
6:41930631:CTAG:C	acceptor_loss	1.0000
6:41930633:A:AG	acceptor_gain	1.0000
6:41930633:AG:A	acceptor_gain	1.0000
6:41930634:G:GG	acceptor_gain	1.0000
6:41930634:GG:G	acceptor_gain	1.0000
6:41930634:GGT:G	acceptor_gain	1.0000
6:41930634:GGTAT:G	acceptor_gain	1.0000
6:41930765:CCAG:C	donor_loss	1.0000
6:41930767:AGGT:A	donor_loss	1.0000
6:41930768:GGTAG:G	donor_loss	1.0000
6:41930769:GTAGA:G	donor_loss	1.0000
6:41930770:T:G	donor_loss	1.0000
6:41931394:A:AG	acceptor_gain	1.0000
6:41931394:A:AT	acceptor_loss	1.0000
6:41931394:AG:A	acceptor_gain	1.0000
6:41931395:G:GG	acceptor_gain	1.0000
6:41931395:GG:G	acceptor_gain	1.0000

AlphaMissense

2817 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
6:41930701:T:A	W213R	1.000
6:41930701:T:C	W213R	1.000
6:41930703:G:C	W213C	1.000
6:41930703:G:T	W213C	1.000
6:41930737:T:A	W225R	1.000
6:41930737:T:C	W225R	1.000
6:41931556:G:A	G289R	1.000
6:41931556:G:C	G289R	1.000
6:41931556:G:T	G289W	1.000
6:41931728:G:A	G289E	1.000
6:41931775:G:C	A305P	1.000
6:41921735:T:C	I58T	0.999
6:41930152:T:A	I151N	0.999
6:41930657:G:A	G198E	0.999
6:41930670:G:C	K202N	0.999
6:41930670:G:T	K202N	0.999
6:41930677:A:G	K205E	0.999
6:41930714:T:A	L217H	0.999
6:41930714:T:C	L217P	0.999
6:41930739:G:C	W225C	0.999
6:41930739:G:T	W225C	0.999
6:41930750:C:A	A229D	0.999
6:41930762:C:A	A233D	0.999
6:41931413:T:C	L241P	0.999
6:41931428:C:A	A246D	0.999
6:41931436:T:C	F249L	0.999
6:41931437:T:C	F249S	0.999
6:41931438:C:A	F249L	0.999
6:41931438:C:G	F249L	0.999
6:41931446:T:C	L252P	0.999

dbSNP variants (sampled 300 via entrez): RS1000020550 (6:41907062 C>A), RS1000020825 (6:41929266 T>G), RS1000053120 (6:41907386 C>A,G,T), RS1000067246 (6:41917979 T>C), RS1000087810 (6:41914761 C>T), RS1000135353 (6:41916654 C>T), RS1000205243 (6:41911209 G>T), RS1000250025 (6:41914444 G>A), RS1000383840 (6:41921265 C>G), RS1000477092 (6:41927228 G>C), RS1000490405 (6:41913041 T>C,G), RS1000847759 (6:41926867 G>A), RS1000919211 (6:41922701 C>A), RS1001041665 (6:41908876 A>G), RS1001236599 (6:41918301 C>T)

Disease associations

OMIM: gene MIM:603871 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

11 associations (top):

Study	Trait	p-value
GCST000498_6	Hematological parameters	7.000000e-19
GCST000503_2	Mean corpuscular volume	1.000000e-31
GCST000504_1	Mean corpuscular hemoglobin	8.000000e-20
GCST000585_11	Mean corpuscular volume	4.000000e-27
GCST000587_12	Mean corpuscular hemoglobin	2.000000e-20
GCST000588_5	Red blood cell count	1.000000e-10
GCST001781_8	Mean corpuscular volume	9.000000e-08
GCST002541_21	Menarche (age at onset)	1.000000e-12
GCST003993_12	Menarche (age at onset)	5.000000e-08
GCST009391_1889	Metabolite levels	6.000000e-07
GCST90002394_155	Monocyte percentage of white cells	1.000000e-13

EFO canonical traits (6, from GWAS)

EFO ID	Trait name
EFO:0004527	mean corpuscular hemoglobin
EFO:0004509	hemoglobin measurement
EFO:0004305	erythrocyte count
EFO:0004703	age at menarche
EFO:0010414	triacylglycerol 52:2 measurement
EFO:0007989	monocyte percentage of leukocytes

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067430 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
5.49	Kd	3263	nM	CHEMBL5653589
5.49	ED50	3263	nM	CHEMBL5653589

PubChem BioAssay actives

1 with measured affinity, of 2 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2147965: Binding affinity to human BYSL incubated for 45 mins by Kinobead based pull down assay	kd	3.2627	uM

CTD chemical–gene interactions

40 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Aflatoxin B1	decreases expression, increases expression, affects expression, affects cotreatment	4
sodium arsenite	affects binding, increases reaction, decreases expression, increases abundance, increases expression	3
Benzo(a)pyrene	affects methylation, increases expression	3
bisphenol A	decreases expression, affects cotreatment, increases methylation	2
Arsenic	affects methylation, increases abundance, increases expression	2
Estradiol	increases expression	2
Nickel	increases expression	2
Tretinoin	decreases expression	2
Cyclosporine	increases expression	2
aristolochic acid I	increases expression	1
3-((6-(2-methoxyphenyl)pyrimidin-4-yl)amino)phenyl)methane sulfonamide	decreases expression	1
GSK-J4	decreases expression	1
FR900359	increases phosphorylation	1
triphenyl phosphate	affects expression	1
deoxynivalenol	increases expression	1
coumarin	increases phosphorylation	1
di-n-butylphosphoric acid	affects expression	1
glycidamide	increases expression	1
CGP 52608	affects binding, increases reaction	1
perfluoro-n-nonanoic acid	increases expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, decreases expression	1
abrine	increases expression	1
LDN 193189	affects cotreatment, decreases expression	1
theaflavin-3,3’-digallate	affects expression	1
Temozolomide	increases expression	1
Fulvestrant	affects cotreatment, increases methylation	1
Air Pollutants	affects expression, increases abundance	1
Ivermectin	decreases expression	1
Ozone	increases abundance, affects expression	1
Phenobarbital	affects expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5651007	Binding	Binding affinity to human BYSL incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.