C16orf82

Summary

C16orf82 (chromosome 16 open reading frame 82, HGNC:30755) is a protein-coding gene on chromosome 16p12.1, encoding Protein TNT (Q7Z2V1).

At a glance

• GWAS associations: 8
• Clinical variants (ClinVar): 11 total
• MANE Select transcript: NM_001145545

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:30755
Approved symbol	C16orf82
Name	chromosome 16 open reading frame 82
Location	16p12.1
Locus type	gene with protein product
Status	Approved
Aliases	TNT
Ensembl gene	ENSG00000234186
Ensembl biotype	protein_coding
Entrez	162083

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 protein_coding

ENST00000505035

RefSeq mRNA: 1 — MANE Select: NM_001145545 NM_001145545

Canonical transcript exons

ENST00000505035 — 1 exons

Exon	Start	End
ENSE00002066194	27066927	27069166

Expression profiles

Bgee: expression breadth broad, 62 present calls, max score 98.09.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 0.8030 / max 811.0508, expressed in 14 samples.

FANTOM5 promoters (4 alternative TSS)

Promoter ID	TPM avg	Samples expressed
153291	0.4324	6
153292	0.3340	8
153290	0.0316	3
153293	0.0050	1

Top tissues by expression

215 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
left testis	UBERON:0004533	98.09	gold quality
right testis	UBERON:0004534	97.87	gold quality
sperm	CL:0000019	97.45	gold quality
testis	UBERON:0000473	95.08	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	89.38	gold quality
adult organism	UBERON:0007023	87.38	gold quality
pancreatic ductal cell	CL:0002079	76.53	silver quality
epithelial cell of pancreas	CL:0000083	76.47	gold quality
buccal mucosa cell	CL:0002336	75.65	silver quality
cardiac muscle of right atrium	UBERON:0003379	73.68	gold quality
left ventricle myocardium	UBERON:0006566	73.52	gold quality
skeletal muscle tissue of rectus abdominis	UBERON:0004511	69.17	gold quality
upper arm skin	UBERON:0004263	68.84	gold quality
kidney epithelium	UBERON:0004819	67.88	gold quality
myocardium	UBERON:0002349	65.87	gold quality
vastus lateralis	UBERON:0001379	62.58	gold quality
quadriceps femoris	UBERON:0001377	62.44	gold quality
gingival epithelium	UBERON:0001949	62.10	gold quality
deltoid	UBERON:0001476	61.54	gold quality
nasal cavity epithelium	UBERON:0005384	61.53	gold quality
vena cava	UBERON:0004087	61.45	gold quality
ileal mucosa	UBERON:0000331	60.87	silver quality
cauda epididymis	UBERON:0004360	60.84	gold quality
gingiva	UBERON:0001828	59.44	gold quality
trabecular bone tissue	UBERON:0002483	59.38	gold quality
superficial temporal artery	UBERON:0001614	58.40	gold quality
tibialis anterior	UBERON:0001385	58.25	silver quality
biceps brachii	UBERON:0001507	57.50	gold quality
layer of synovial tissue	UBERON:0007616	57.10	gold quality
skeletal muscle tissue of biceps brachii	UBERON:0004502	56.35	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-GEOD-134144	yes	32.68
E-ANND-3	no	1.48

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

67 targeting C16orf82, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3134	100.00	66.43	777
HSA-MIR-4776-3P	100.00	68.73	1340
HSA-MIR-4534	99.99	66.58	1907
HSA-MIR-27A-3P	99.98	72.13	2955
HSA-MIR-27B-3P	99.98	72.13	2955
HSA-MIR-9985	99.98	72.11	2939
HSA-MIR-3148	99.97	75.06	6478
HSA-MIR-6778-3P	99.96	67.29	2693
HSA-MIR-8082	99.95	67.27	1170
HSA-MIR-651-3P	99.94	73.48	5177
HSA-MIR-6721-5P	99.93	68.92	2981
HSA-MIR-10395-5P	99.86	67.35	676
HSA-MIR-221-3P	99.86	71.56	1329
HSA-MIR-222-3P	99.86	71.35	1337
HSA-MIR-2052	99.79	69.37	2031
HSA-MIR-187-5P	99.74	70.26	1404
HSA-MIR-378G	99.71	64.90	1106
HSA-MIR-29B-2-5P	99.67	68.98	1726
HSA-MIR-6762-3P	99.66	66.94	1188
HSA-MIR-3685	99.62	68.83	1621
HSA-MIR-4310	99.59	68.84	2527
HSA-MIR-7112-5P	99.59	65.76	104
HSA-MIR-182-3P	99.57	67.57	825
HSA-MIR-2392	99.43	67.50	708
HSA-MIR-19A-5P	99.36	66.93	1675
HSA-MIR-19B-1-5P	99.36	67.07	1669
HSA-MIR-19B-2-5P	99.36	67.07	1669
HSA-MIR-3064-5P	99.26	66.13	1497
HSA-MIR-3085-3P	99.26	66.16	1490
HSA-MIR-6504-5P	99.26	65.95	1487

Cross-species orthologs

0 orthologs

Protein

Protein identifiers

Protein TNT — Q7Z2V1 (reviewed: Q7Z2V1)

All UniProt accessions (1): A0A5F9ZHF3

UniProt curated annotations — full annotation on UniProt →

Tissue specificity. Preferentially expressed in teratocarcinoma rather than in normal testis.

RefSeq proteins (1): NP_001139017* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR031520	DUF4694	Family

Pfam: PF15765

UniProt features (8 total): compositionally biased region 6, chain 1, region of interest 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q7Z2V1-F1	57.55	0.00

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 10 (showing top): chr16p12, MIR651_3P, MIR5701, MIR5089_5P, MIR6762_3P, MIR380_5P, GSE15659_NONSUPPRESSIVE_TCELL_VS_ACTIVATED_TREG_UP, GSE17974_IL4_AND_ANTI_IL12_VS_UNTREATED_0.5H_ACT_CD4_TCELL_DN, HE_LIM_SUN_FETAL_LUNG_C7_PROLIFERATING_SCHWANN_CELL, GSE21546_UNSTIM_VS_ANTI_CD3_STIM_SAP1A_KO_DP_THYMOCYTES_DN

GO Biological Process (0):

GO Molecular Function (0):

GO Cellular Component (0):

Protein interactions and networks

STRING

36 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
C16orf82	ETHE1	O95571	348
C16orf82	ACTN2	P35609	322
C16orf82	LYSMD3	Q7Z3D4	320
C16orf82	SCAF8	Q9UPN6	271
C16orf82	TIMM10B	Q9Y5J6	270
C16orf82	TNNT1	P13805	269
C16orf82	APIP	Q96GX9	232
C16orf82	TIMM10	P62072	217
C16orf82	SDF4	Q9BRK5	207
C16orf82	CITED4	Q96RK1	194
C16orf82	UBE2H	P37286	191
C16orf82	CLK4	Q9HAZ1	183
C16orf82	MYF5	P13349	166
C16orf82	MYOD1	P15172	164
C16orf82	CDC14A	Q9UNH5	162

IntAct

0 interactions, top by confidence:

BioGRID (1): C16orf82 (Cross-Linking-MS (XL-MS))

ESM2 similar proteins: A0JNH1, A6NCI8, A6NGY1, A6NKB5, A6X8Z5, D3Z1D3, E9Q0C6, E9Q286, E9Q3S4, E9Q7D5, M0RD54, O14513, O88466, O94854, P46013, Q05860, Q0VAV2, Q0VF49, Q12774, Q1RMX6, Q2M3C7, Q32LI3, Q3MHH3, Q3MJ40, Q3URK3, Q3V0A6, Q49AJ0, Q4V7J0, Q5DU28, Q5F2C3, Q5VV67, Q66H53, Q68DA7, Q6NZN1, Q711Q0, Q7TSG5, Q7Z2V1, Q80VY2, Q80W69, Q86TB3

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

11 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	9
Likely benign	2
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

0 predictions. Top by Δscore:

AlphaMissense

1012 scored. Top likely-pathogenic:

dbSNP variants (sampled 300 via entrez): RS1000118879 (16:27067912 A>G), RS1000446759 (16:27069189 C>T), RS1002062646 (16:27068665 C>T), RS1003415294 (16:27066846 A>C), RS1003679959 (16:27067865 C>T), RS1005015688 (16:27069625 T>C), RS1006306670 (16:27066222 C>A,G), RS1006687320 (16:27066374 C>T), RS1007534416 (16:27067923 T>C), RS1008225665 (16:27068997 C>T), RS1008672921 (16:27068801 C>T), RS1008975478 (16:27067896 A>G), RS1009149736 (16:27068696 C>T), RS1009732816 (16:27069334 C>T), RS1010100962 (16:27067964 C>T)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

8 associations (top):

Study	Trait	p-value
GCST002701_1	Verbal declarative memory	3.000000e-08
GCST002701_16	Verbal declarative memory	1.000000e-07
GCST004311_1	Facial morphology (factor 7, width of cartilaginous portion of nose)	2.000000e-08
GCST006427_52	Depression in smokers	5.000000e-06
GCST008152_155	Weight	6.000000e-06
GCST008157_21	Body fat mass	8.000000e-06
GCST009391_787	Metabolite levels	4.000000e-07
GCST009798_67	Asthma	5.000000e-11

EFO canonical traits (4, from GWAS)

EFO ID	Trait name
EFO:0004874	memory performance
EFO:0006805	word list delayed recall measurement
EFO:0004338	body weight
EFO:0010395	sphingomyelin 22:0 measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

3 total (human), top 3 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
sodium arsenite	increases expression	1
Benzo(a)pyrene	decreases methylation	1
Valproic Acid	increases methylation	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.