CEBPZ

Summary

CEBPZ (CCAAT enhancer binding protein zeta, HGNC:24218) is a protein-coding gene on chromosome 2p22.2, encoding CCAAT/enhancer-binding protein zeta (Q03701). Stimulates transcription from the HSP70 promoter. It is a common-essential gene (DepMap: required in 93.3% of cancer cell lines).

This gene belongs to the CBF/Mak21 family. The encoded protein plays a role in cellular response to environmental stimuli through a transcriptional process that involves heat shock factors, conserved DNA elements (heat shock elements or HSEs) and CCAAT boxes. The protein acts as a DNA-binding transcriptional activator and regulates the heat-shock protein 70 (HSP70) promoter in a CCAAT-dependent manner. The protein is also involved in cell growth and differentiation, particularly, hematopoietic differentiation.

Source: NCBI Gene 10153 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 196 total
• GTEx eGene: eQTL in 46 tissues, sQTL in 1 (q < 0.05)
• Cancer dependency (DepMap): dependent in 93.3% of screened cell lines (common-essential)
• Constraint (gnomAD): pLI 0.00 (LoF-tolerant), LOEUF 0.92 (unconstrained)
• Transcription factor: yes — 20 downstream targets (CollecTRI)
• MANE Select transcript: NM_005760
• Interaction hub: 1,977 STRING partners (top 8% of genes corpus-wide)
• Notable: 196 clinical variants but no curated precision-oncology (CIViC) evidence yet

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:24218
Approved symbol	CEBPZ
Name	CCAAT enhancer binding protein zeta
Location	2p22.2
Locus type	gene with protein product
Status	Approved
Aliases	CBF2, CTF2, HSP-CBF
Ensembl gene	ENSG00000115816
Ensembl biotype	protein_coding
OMIM	612828
Entrez	10153

Gene structure

Transcript identifiers

Ensembl transcripts: 16 — 10 protein_coding, 5 nonsense_mediated_decay, 1 retained_intron

MANE Select: ENST00000234170

Transcript support level (Ensembl TSL; 1 = fully mRNA-supported): TSL 1: 1, TSL 2: 1, TSL 3: 1

ENST00000234170, ENST00000446769, ENST00000489306, ENST00000898578, ENST00000898579, ENST00000938546, ENST00000938547, ENST00000938548, ENST00000994133, ENST00001086106, ENST00001086107, ENST00001086109, ENST00001115050, ENST00001119818, ENST00001127289, ENST00001145026

RefSeq mRNA: 1 — MANE Select: NM_005760 NM_005760

CCDS: CCDS1787

Canonical transcript exons

ENST00000234170 — 16 exons

Exon	Start	End
ENSE00000747278	37211843	37212039
ENSE00000747280	37212335	37212392
ENSE00000747281	37213864	37213961
ENSE00000747282	37214886	37214952
ENSE00000747283	37216140	37216208
ENSE00000747285	37216316	37216418
ENSE00000747287	37216984	37217037
ENSE00000747288	37220385	37220473
ENSE00000747289	37222380	37222563
ENSE00000747290	37223170	37223401
ENSE00000747292	37227544	37229036
ENSE00001282630	37231412	37231596
ENSE00001390774	37201612	37201903
ENSE00003502984	37202950	37203008
ENSE00003616242	37202784	37202865
ENSE00003671204	37210999	37211082

Expression profiles

Bgee: expression breadth ubiquitous, 293 present calls, max score 97.56.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 40.1153 / max 997.1908, expressed in 1802 samples.

Highest expression (Bgee): calcaneal tendon, tendon, tendon of biceps brachii, ventricular zone, adrenal tissue.

Genetic regulation across tissues (GTEx eQTL / sQTL)

CEBPZ is an eGene in GTEx v10 — its expression is genetically regulated (eQTL) in 46 tissues and its splicing (sQTL) in 1 (q < 0.05). Strongest associations first; slope = normalized effect of the lead variant’s alternative allele.

showing 20 of 47 significant tissue × QTL associations:

Tissue	QTL	q-value	Slope	Lead variant
Cells EBV-transformed lymphocytes	eqtl	5.4e-45	-0.51	2:37231568:G:A
Nerve Tibial	eqtl	9.7e-44	-0.30	2:37231568:G:A
Cells Cultured fibroblasts	eqtl	2.9e-39	-0.20	2:37231568:G:A
Thyroid	eqtl	5.1e-37	-0.28	2:37231568:G:A
Skin Sun Exposed Lower leg	eqtl	6.3e-37	-0.24	2:37299694:A:C
Testis	eqtl	9.5e-33	-0.29	2:37231568:G:A
Adipose Subcutaneous	eqtl	2.6e-30	-0.26	2:37231568:G:A
Skin Not Sun Exposed Suprapubic	eqtl	1.5e-27	-0.22	2:37239539:A:G
Esophagus Mucosa	eqtl	1.7e-27	-0.19	2:37231568:G:A
Breast Mammary Tissue	eqtl	6.4e-25	-0.28	2:37231568:G:A
Muscle Skeletal	eqtl	3.8e-23	-0.16	2:37231568:G:A
Artery Tibial	eqtl	3.8e-23	-0.23	2:37231568:G:A
Lung	eqtl	8.5e-22	-0.21	2:37272146:T:C
Adipose Visceral Omentum	eqtl	1.7e-21	-0.17	2:37233557:G:A
Esophagus Muscularis	eqtl	1.4e-19	-0.18	2:37231568:G:A
Colon Sigmoid	eqtl	1.8e-17	-0.22	2:37227075:C:G
Brain Spinal cord cervical c-1	eqtl	3.0e-15	-0.48	2:37294707:G:C
Pancreas	eqtl	1.6e-14	-0.25	2:37231568:G:A
Esophagus Gastroesophageal Junction	eqtl	2.0e-14	-0.19	2:37239539:A:G
Ovary	eqtl	1.5e-13	-0.31	2:37231568:G:A

FANTOM5 promoters

1 alternative TSS:

Promoter ID	TPM avg	Samples expressed
27773	40.1153	1802

Top tissues by expression

showing 100 of 295 tissues by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
calcaneal tendon	UBERON:0003701	97.56	gold quality
tendon	UBERON:0000043	97.38	gold quality
tendon of biceps brachii	UBERON:0008188	97.28	gold quality
ventricular zone	UBERON:0003053	95.32	gold quality
adrenal tissue	UBERON:0018303	95.24	gold quality
medial globus pallidus	UBERON:0002477	94.79	gold quality
left testis	UBERON:0004533	94.12	gold quality
gastrocnemius	UBERON:0001388	94.02	gold quality
testis	UBERON:0000473	93.97	gold quality
right testis	UBERON:0004534	93.88	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	93.72	gold quality
colonic epithelium	UBERON:0000397	93.63	gold quality
pericardium	UBERON:0002407	93.40	gold quality
ganglionic eminence	UBERON:0004023	93.38	gold quality
body of pancreas	UBERON:0001150	93.32	gold quality
islet of Langerhans	UBERON:0000006	93.29	gold quality
muscle of leg	UBERON:0001383	93.28	gold quality
skeletal muscle tissue of rectus abdominis	UBERON:0004511	93.25	gold quality
parietal pleura	UBERON:0002400	93.11	gold quality
cervix squamous epithelium	UBERON:0006922	93.04	gold quality
right adrenal gland cortex	UBERON:0035827	93.01	gold quality
endothelial cell	CL:0000115	92.97	gold quality
adrenal cortex	UBERON:0001235	92.89	gold quality
cranial nerve II	UBERON:0000941	92.85	gold quality
adrenal gland	UBERON:0002369	92.85	gold quality
globus pallidus	UBERON:0001875	92.83	gold quality
pancreas	UBERON:0001264	92.81	gold quality
hindlimb stylopod muscle	UBERON:0004252	92.80	gold quality
left adrenal gland	UBERON:0001234	92.77	gold quality
mucosa of transverse colon	UBERON:0004991	92.75	gold quality
right adrenal gland	UBERON:0001233	92.72	gold quality
left adrenal gland cortex	UBERON:0035825	92.71	gold quality
pleura	UBERON:0000977	92.51	gold quality
muscle organ	UBERON:0001630	92.39	gold quality
visceral pleura	UBERON:0002401	92.30	gold quality
cortical plate	UBERON:0005343	92.17	gold quality
amniotic fluid	UBERON:0000173	92.13	gold quality
ovary	UBERON:0000992	92.02	gold quality
endometrium	UBERON:0001295	91.88	gold quality
left ovary	UBERON:0002119	91.86	gold quality
left uterine tube	UBERON:0001303	91.83	gold quality
mucosa of sigmoid colon	UBERON:0004993	91.83	gold quality
adenohypophysis	UBERON:0002196	91.82	gold quality
right ovary	UBERON:0002118	91.69	gold quality
sigmoid colon	UBERON:0001159	91.65	gold quality
adipose tissue of abdominal region	UBERON:0007808	91.64	gold quality
peritoneum	UBERON:0002358	91.63	gold quality
omental fat pad	UBERON:0010414	91.62	gold quality
muscle layer of sigmoid colon	UBERON:0035805	91.62	gold quality
cartilage tissue	UBERON:0002418	91.38	gold quality
right lung	UBERON:0002167	91.35	gold quality
biceps brachii	UBERON:0001507	91.25	gold quality
monocyte	CL:0000576	91.23	gold quality
connective tissue	UBERON:0002384	91.21	gold quality
blood vessel layer	UBERON:0004797	91.14	gold quality
adipose tissue	UBERON:0001013	91.09	gold quality
tibial nerve	UBERON:0001323	91.07	gold quality
tonsil	UBERON:0002372	91.05	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	91.04	gold quality
mononuclear cell	CL:0000842	90.96	gold quality
colonic mucosa	UBERON:0000317	90.96	gold quality
ectocervix	UBERON:0012249	90.95	gold quality
leukocyte	CL:0000738	90.86	gold quality
upper lobe of left lung	UBERON:0008952	90.81	gold quality
cauda epididymis	UBERON:0004360	90.80	gold quality
colon	UBERON:0001155	90.78	gold quality
large intestine	UBERON:0000059	90.70	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	90.69	gold quality
choroid plexus epithelium	UBERON:0003911	90.68	gold quality
embryo	UBERON:0000922	90.62	gold quality
uterus	UBERON:0000995	90.62	gold quality
rectum	UBERON:0001052	90.58	gold quality
esophagus squamous epithelium	UBERON:0006920	90.58	gold quality
upper lobe of lung	UBERON:0008948	90.58	gold quality
female reproductive system	UBERON:0000474	90.56	gold quality
parotid gland	UBERON:0001831	90.55	gold quality
pituitary gland	UBERON:0000007	90.53	gold quality
esophagus mucosa	UBERON:0002469	90.52	gold quality
mucosa of paranasal sinus	UBERON:0005030	90.51	gold quality
intestine	UBERON:0000160	90.48	gold quality
superior surface of tongue	UBERON:0007371	90.48	gold quality
tibial artery	UBERON:0007610	90.48	gold quality
popliteal artery	UBERON:0002250	90.46	gold quality
subcutaneous adipose tissue	UBERON:0002190	90.43	gold quality
esophagus	UBERON:0001043	90.40	gold quality
right lobe of liver	UBERON:0001114	90.39	gold quality
pigmented layer of retina	UBERON:0001782	90.36	gold quality
transverse colon	UBERON:0001157	90.30	gold quality
small intestine Peyer’s patch	UBERON:0003454	90.28	gold quality
saphenous vein	UBERON:0007318	90.28	gold quality
skeletal muscle tissue	UBERON:0001134	90.25	gold quality
body of uterus	UBERON:0009853	90.24	gold quality
prostate gland	UBERON:0002367	90.22	gold quality
vagina	UBERON:0000996	90.20	gold quality
trabecular bone tissue	UBERON:0002483	90.17	gold quality
lower esophagus muscularis layer	UBERON:0035833	90.16	gold quality
oral cavity	UBERON:0000167	90.15	gold quality
deltoid	UBERON:0001476	90.13	silver quality
lower esophagus	UBERON:0013473	90.12	gold quality
vena cava	UBERON:0004087	90.08	silver quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	10.20
E-MTAB-4850	no	673.52
E-CURD-135	no	480.50

HPA expression

RNA tissue specificity: Low tissue specificity

showing 60 of 76 HPA tissues / cells by nTPM:

Tissue / cell	Axis	nTPM
Testis	tissue	35.9
Bone marrow	tissue	29.5
Corpus callosum	tissue	28.1
Adrenal gland	tissue	26.3
Midbrain	tissue	25.3
Liver	tissue	25.3
Thalamus	tissue	25.2
Spinal cord	tissue	25.2
Urinary bladder	tissue	24.8
Breast	tissue	24.5
Pons	tissue	24.4
Superior colliculi	tissue	24.4
Putamen	tissue	23.8
Medulla oblongata	tissue	23.8
Kidney	tissue	23.7
Choroid plexus	tissue	23.3
Hippocampal formation	tissue	23.3
Lymph node	tissue	23.0
Tonsil	tissue	22.9
Tongue	tissue	22.7
Retina	tissue	22.6
Substantia nigra	tissue	22.6
Thymus	tissue	22.4
Cerebellum	tissue	22.1
Ovary	tissue	21.8
Placenta	tissue	21.6
Periaqueductal gray	tissue	21.2
Septum	tissue	21.1
Parathyroid gland	tissue	21.0
Thyroid gland	tissue	20.9
Ventral forebrain	tissue	20.5
Cerebral cortex	tissue	20.4
Skeletal muscle	tissue	20.4
Hypothalamus	tissue	20.4
Somatosensory cortex	tissue	20.4
Occipital cortex	tissue	20.3
Nucleus accumbens	tissue	20.1
Postcentral gyrus	tissue	19.9
Olfactory bulb	tissue	19.8
Adipose tissue	tissue	19.7
Pancreas	tissue	19.5
Esophagus	tissue	19.2
Locus coeruleus	tissue	19.1
Entorhinal cortex	tissue	19.0
Rectum	tissue	18.8
Endometrium 1	tissue	18.8
Epididymis	tissue	18.8
Basal ganglia	tissue	18.8
Spleen	tissue	18.7
Prostate	tissue	18.5
Prefrontal cortex	tissue	18.4
Colon	tissue	18.3
Motor cortex	tissue	18.3
Lung	tissue	18.2
Retrosplenial and cingulate cortex	tissue	18.1
Appendix	tissue	17.9
Temporal cortex	tissue	17.9
Retrosplenial cortex	tissue	17.6
Small intestine	tissue	17.0
Cervix	tissue	16.9

Brain sub-regions (HPA Brain Atlas): detected in 189; highest nTPM — Cerebellar cortex (31.1), Flocculonodular lobe (29.1), Vermis (28.8), White matter (28.1), Corpus callosum, splenium (white matter) (27.0).

Regulation

Is transcription factor: yes

Downstream targets (CollecTRI)

20 targets:

Target	Regulation
ALOX12
APC	Activation
CDKN1A
COL10A1
COL11A1	Repression
COL1A2	Unknown
COL5A3	Activation
CP	Unknown
CYBB
ERVW-1
FAS
GZMB	Unknown
HSPA4	Activation
IL3
MLH1	Repression
PDLIM7	Activation
SOX9	Unknown
THBS1
TP73
TPM1

JASPAR motifs

Motif	Name	Family
UN0800.1	CEBPZ	CEBP-related

Upstream regulators (CollecTRI, top): JUND, RBPJ, TCF3

miRNA regulators (miRDB)

Targeting CEBPZ by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific).

18 miRNAs:

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3613-3P	100.00	76.36	7965
HSA-MIR-520D-5P	99.98	73.34	4883
HSA-MIR-524-5P	99.98	73.43	4882
HSA-MIR-548P	99.98	72.25	3784
HSA-MIR-590-3P	99.96	74.34	6478
HSA-MIR-545-3P	99.95	70.74	2783
HSA-MIR-1-3P	99.93	72.35	1914
HSA-MIR-206	99.93	72.50	1893
HSA-MIR-613	99.91	71.50	1710
HSA-MIR-556-3P	99.74	68.75	1203
HSA-MIR-3120-3P	99.54	70.28	2669
HSA-MIR-6128	99.33	67.83	1581
HSA-MIR-10399-5P	99.17	69.87	2610
HSA-MIR-6504-3P	99.17	69.31	2891
HSA-MIR-181A-2-3P	98.91	67.60	1168
HSA-MIR-6509-3P	98.32	67.33	1343
HSA-MIR-5694	97.06	67.70	682
HSA-MIR-758-5P	93.99	64.46	534

Functional genomics

gnomAD constraint: pLI 3.64e-14, LOEUF 0.923, missense Z -1.63 — population loss-of-function intolerance (pLI ≥ 0.9 = LoF-intolerant; lower LOEUF = more constrained). gnomAD

Regional missense constraint (gnomAD RMC): no regional variation along ENST00000234170 — one region, missense o/e 1.17. DepMap (CRISPR cell-line fitness): dependent in 93.3% of screened cell lines, common-essential.

Most-dependent cell lines (DepMap gene-effect, lower = stronger; ≤ −0.5 shown): PA-TU-8988T (-1.818), OCI-AML2 (-1.745), NOMO-1 (-1.666), C32 (-1.664), G-402 (-1.64), SCC-25 (-1.632), SF539 (-1.57), TM-87 (-1.56), NCI-H1792 (-1.544), KE-97 (-1.499), KP-4 (-1.499), U-87 MG (-1.44), M059K (-1.422), BHT-101 (-1.361), OPM-2 (-1.359).

Literature-anchored findings (GeneRIF, showing 1)

• Mouse and Human CCAAT binding factors are discussed in this citation. (PMID:8604343)

Cross-species orthologs

6 orthologs

Organism	Symbol	Gene ID
danio_rerio	cebpz	ENSDARG00000045857
danio_rerio	si:ch1073-412h12.3	ENSDARG00000094486
mus_musculus	Cebpz	ENSMUSG00000024081
rattus_norvegicus	Cebpz	ENSRNOG00000005087
drosophila_melanogaster	Noc1	FBGN0036124
caenorhabditis_elegans		WBGENE00009084

Mouse-model phenotypes

Observed phenotypes of the mouse ortholog (MGI:109386) from MGI (Alliance of Genome Resources) — knockout/mutant phenotypes, ranked by annotation count. 1 distinct phenotype terms.

Mouse phenotype (MP)	Records
increased total body fat amount	1

Additional cross-species homologs

Sequence homologs in species beyond Ensembl Compara’s model organisms, by Diamond local protein alignment (% sequence identity; best hit per species). Lower-identity rows are more distant homologs. The matched accessions’ gene symbols aren’t in biobtree yet, so each row shows the UniProt accession.

Organism	UniProt	Identity	Method
Saccharomyces cerevisiae S288C	Q12176	41.1%	Diamond
Schizosaccharomyces pombe 972h-	O36021	40.1%	Diamond
Thermochaetoides thermophila DSM 1495	G0SEQ5	38.8%	Diamond
Arabidopsis thaliana	F4IDC2	33.8%	Diamond

Genomic neighbors (locus context)

Genes flanking this one on the chromosome (NCBI/Entrez gene neighborhood). Positional context only — some neighbors share regulatory elements (bidirectional promoters, antisense/overlapping transcripts), but chromosomal adjacency is not itself a functional relationship (see Interactions for that).

Symbol	Type	Entrez
CEBPZOS	protein-coding	100505876
PRKD3	protein-coding	23683
GPATCH11	protein-coding	253635
SULT6B1	protein-coding	391365
NDUFAF7	protein-coding	55471
EIF2AK2	protein-coding	5610
PRKD3-DT	ncRNA	101929542
QPCT	protein-coding	25797
HEATR5B	protein-coding	54497
STRN	protein-coding	6801

Protein

Protein identifiers

CCAAT/enhancer-binding protein zeta — Q03701 (reviewed: Q03701)

Alternative names: CCAAT-box-binding transcription factor

All UniProt accessions (2): C9J9W7, Q03701

UniProt curated annotations — full annotation on UniProt →

Function. Stimulates transcription from the HSP70 promoter.

Subcellular location. Nucleus.

Similarity. Belongs to the CBF/MAK21 family.

RefSeq proteins (1): NP_005751* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR005612	CCAAT-binding_factor	Domain
IPR016024	ARM-type_fold	Homologous_superfamily
IPR040155	CEBPZ/Mak21-like	Family

Pfam: PF03914

UniProt features (24 total): modified residue 7, compositionally biased region 6, region of interest 5, sequence variant 4, chain 1, sequence conflict 1

Structure

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q03701-F1	67.34	0.15

Human Protein Atlas

Protein class: Predicted intracellular proteins, Essential proteins

Protein evidence: Evidence at protein level

Subcellular location (HPA, imaging): nucleoplasm; vesicles

Antibody reliability (HPA): IH uncertain · IF supported

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (7)

Type	Positions
Phosphoserine	113, 629, 835, 959, 973, 978
N6-acetyllysine	695

Function

Pathways and Gene Ontology

Reactome pathways

0 curated (TAS) of 0; 0 disease pathways. Curated-first; evidence = TAS (manually curated) vs IEA (electronic inference).

MSigDB gene sets: 154 (showing top): GSE45365_NK_CELL_VS_CD8A_DC_MCMV_INFECTION_DN, GSE18804_SPLEEN_MACROPHAGE_VS_TUMORAL_MACROPHAGE_UP, RODRIGUES_THYROID_CARCINOMA_ANAPLASTIC_UP, RODRIGUES_THYROID_CARCINOMA_POORLY_DIFFERENTIATED_UP, MODULE_255, MODULE_317, MORF_HDAC2, PUJANA_CHEK2_PCC_NETWORK, SCHUHMACHER_MYC_TARGETS_UP, MUELLER_PLURINET, DEURIG_T_CELL_PROLYMPHOCYTIC_LEUKEMIA_DN, MORF_RFC4, ACATTCC_MIR1_MIR206, MORF_PRKDC, GARY_CD5_TARGETS_DN

GO Biological Process (2): DNA-templated transcription (GO:0006351), positive regulation of transcription by RNA polymerase II (GO:0045944)

GO Molecular Function (3): transcription coactivator activity (GO:0003713), RNA binding (GO:0003723), protein binding (GO:0005515)

GO Cellular Component (3): nucleus (GO:0005634), nucleoplasm (GO:0005654), CCAAT-binding factor complex (GO:0016602)

GO top-level categories

12 GO namespaces:

Category	Terms
positive regulation of DNA-templated transcription	2
gene expression	1
RNA biosynthetic process	1
regulation of transcription by RNA polymerase II	1
transcription by RNA polymerase II	1
transcription coregulator activity	1
nucleic acid binding	1
binding	1
intracellular membrane-bounded organelle	1
nuclear lumen	1
cellular anatomical structure	1
RNA polymerase II transcription regulator complex	1

Experimentally-supported GO annotations

GO terms backed by direct experimental evidence (ECO experimental codes) — 5 of 8 annotations. The rest are phylogenetic / computational / electronic (IEA).

5 experimentally-supported GO terms:

GO term	NS	Evidence
positive regulation of transcription by RNA polymerase II	BP	direct assay
nucleoplasm	CC	direct assay
nucleus	CC	direct assay
RNA binding	MF	high-throughput assay
transcription coactivator activity	MF	genetic interaction

Protein interactions and networks

STRING

showing 15 of 1,977 interactions by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
CEBPZ	RBPJ	Q06330	908
CEBPZ	CDC42EP3	Q9UKI2	768
CEBPZ	SKP1	P34991	750
CEBPZ	CETN1	Q12798	735
CEBPZ	CTF1	Q16619	715
CEBPZ	PDCD11	Q14690	695
CEBPZ	RPH3AL	Q9UNE2	654
CEBPZ	NSA2	O95478	593
CEBPZ	GNL2	Q13823	585
CEBPZ	WDR12	Q9GZL7	585
CEBPZ	WDR74	Q6RFH5	576
CEBPZ	URB1	O60287	559
CEBPZ	MYB	P10242	538
CEBPZ	ICE1	Q9Y2F5	538
CEBPZ	CDH2	P19022	534

IntAct

Human interaction partners of CEBPZ in IntAct, one row per partner (best MIscore; records = supporting IntAct interaction records).

20 partners by confidence:

Partner	Interaction type	MIscore	Records
GSK3B	physical association	0.550	1
PRKRA	physical association	0.550	1
TP53	physical association	0.520	2
MFHAS1	direct interaction	0.440	1
TP73	physical association	0.400	2
PROX1	physical association	0.400	1
SF3B2	physical association	0.400	1
TMF1	physical association	0.400	1
HMOX2	physical association	0.370	1
LAMTOR3	physical association	0.370	1
LTB4R2	physical association	0.370	1
LZTR1	physical association	0.370	1
PCBD1	physical association	0.370	1
RPS6KA6	physical association	0.370	1
RXRG	physical association	0.370	1
STAT6	physical association	0.370	1
UBE3A	physical association	0.370	1
YY1AP1	association	0.350	2
CBFB	physical association	0.000	1
MYC	physical association	0.000	1

BioGRID (331 interactions; top human partners by supporting records): NOC2L (Co-fractionation; 4 records), EGFR (Affinity Capture-MS; 3 records), FMR1NB (Affinity Capture-MS; 3 records), NPM1 (Affinity Capture-MS; 3 records), RRP8 (Affinity Capture-MS; 3 records), THAP7 (Affinity Capture-MS; 3 records), VAPB (Affinity Capture-MS; 3 records), WDR46 (Affinity Capture-MS; 3 records), ADARB1 (Affinity Capture-MS; 2 records), ANLN (Affinity Capture-MS; 2 records), APEX1 (Affinity Capture-RNA; 2 records), CCDC59 (Affinity Capture-MS; 2 records), EBNA1BP2 (Co-fractionation; 2 records), FBL (Proximity Label-MS; 2 records), FGF3 (Affinity Capture-MS; 2 records)

Disease & clinical

Genetic variants

Variant evidence for CEBPZ: 196 ClinVar records (0 pathogenic / likely pathogenic); 1 GWAS association.

ClinVar

196 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	163
Likely benign	12
Benign	2

SpliceAI

Top 10 splice-impact sites by Δscore (adjacent positions with the same effect merged into one range; 39 predictions of 1,200):

Position(s)	Effect	Δscore	Sites
2:37202782–37202783	donor_loss	1.0000	4
2:37202866–37202867	acceptor_loss	1.0000	4
2:37202867–37202869	acceptor_gain	1.0000	2
2:37202948–37202949	donor_loss	1.0000	5
2:37203009–37203010	acceptor_loss	1.0000	3
2:37211842	donor_loss	1.0000	2
2:37212040–37212041	acceptor_loss	1.0000	4
2:37214884–37214885	donor_loss	1.0000	5
2:37214953–37214954	acceptor_loss	1.0000	5
2:37216138–37216139	donor_loss	1.0000	5

AlphaMissense

1,456 missense substitutions predicted likely pathogenic (of 7,109 scored), across 426 residues. Top 20 hotspot residues, ranked by the number of likely-pathogenic substitutions (of those reachable by a single-nucleotide change), then by mean score:

Residue	Likely-pathogenic substitutions	Mean am_pathogenicity	Max am_pathogenicity
M416	9	0.838	0.972
D246	8	0.973	0.992
D577	8	0.962	0.991
F755	8	0.962	0.998
H724	8	0.957	0.995
D749	8	0.947	0.982
D393	8	0.943	0.989
F759	8	0.937	0.994
F469	8	0.915	0.976
D54	8	0.893	0.953
D757	8	0.893	0.972
H413	8	0.888	0.985
H722	8	0.862	0.964
F307	8	0.785	0.937
W1015	7	0.970	0.993
F730	7	0.956	0.981
F512	7	0.955	0.981
R589	7	0.951	0.994
W234	7	0.943	0.989
E715	7	0.942	0.981

HPA cancer prognostics

RNA cancer specificity: Low cancer specificity

Human Protein Atlas survival analysis — favorable = higher expression predicts better survival.

5 prognostic cancer types:

Cancer	Prognostic	p-value
Kidney Renal Clear Cell Carcinoma (TCGA)	favorable	1.61e-07
Pancreatic Adenocarcinoma (validation)	unfavorable	4.28e-05
Rectum Adenocarcinoma (TCGA)	favorable	0.000248
Liver Hepatocellular Carcinoma (validation)	unfavorable	0.000326
Liver Hepatocellular Carcinoma (TCGA)	unfavorable	0.000955

Disease associations

OMIM: gene MIM:612828 | disease phenotypes:

GWAS associations

1 associations:

Study	Trait	p-value
GCST002149_12	Schizophrenia	7e-9

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067231 (SINGLE PROTEIN)

PharmGKB: 1 entry (CPIC guideline: no)

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
5.49	Kd	3243	nM	CHEMBL3752910
5.49	ED50	3243	nM	CHEMBL3752910

PubChem BioAssay actives

1 with measured affinity, of 2 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(1-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2149838: Binding affinity to human CEBPZ incubated for 45 mins by Kinobead based pull down assay	kd	3.2431	uM

CTD chemical–gene interactions

35 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
entinostat	affects cotreatment, increases expression	2
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, decreases expression, increases expression	2
Estradiol	increases expression, affects cotreatment, decreases expression	2
Tretinoin	decreases expression, increases expression	2
Valproic Acid	decreases expression	2
Cyclosporine	increases expression	2
aristolochic acid I	decreases expression	1
FR900359	increases phosphorylation	1
TAK-243	decreases sumoylation	1
dicrotophos	decreases expression	1
triphenyl phosphate	affects expression	1
bisphenol A	decreases expression	1
dorsomorphin	affects cotreatment, increases expression	1
jinfukang	decreases expression	1
LDN 193189	affects cotreatment, decreases expression	1
Temozolomide	increases expression	1
Arsenic Trioxide	increases expression	1
Air Pollutants	decreases expression, increases abundance	1
Camptothecin	decreases expression	1
Daunorubicin	decreases expression	1
Dichlorodiphenyl Dichloroethylene	increases expression	1
Bucladesine	affects cotreatment, decreases expression	1
Dinitrochlorobenzene	affects binding	1
Etoposide	decreases expression	1
Ivermectin	decreases expression	1
Polychlorinated Biphenyls	affects expression	1
Ribonucleotides	affects binding	1
Tetrachlorodibenzodioxin	decreases expression	1
Tobacco Smoke Pollution	increases expression	1
Aflatoxin M1	decreases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5652880	Binding	Binding affinity to human CEBPZ incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — *ChemMedChem*

Clinical trials (associated diseases)

Via MONDO — disease-level, not drug-specific.

Predicted chemistry — Sugi Predict

The predicted patent-compound landscape for CEBPZ across ~30M SureChEMBL compounds — explore on Sugi Predict.