CHCHD6

Summary

CHCHD6 (coiled-coil-helix-coiled-coil-helix domain containing 6, HGNC:28184) is a protein-coding gene on chromosome 3q21.3, encoding MICOS complex subunit MIC25 (Q9BRQ6). Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.

Involved in DNA damage response and cristae formation. Located in cytosol and mitochondrial inner membrane. Part of MICOS complex.

Source: NCBI Gene 84303 — RefSeq curated summary.

At a glance

• GWAS associations: 4
• Clinical variants (ClinVar): 52 total
• Druggable target: yes
• MANE Select transcript: NM_032343

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:28184
Approved symbol	CHCHD6
Name	coiled-coil-helix-coiled-coil-helix domain containing 6
Location	3q21.3
Locus type	gene with protein product
Status	Approved
Aliases	MGC13016, PPP1R23, CHCM1, Mic25, MICOS25
Ensembl gene	ENSG00000159685
Ensembl biotype	protein_coding
OMIM	615634
Entrez	84303

Gene structure

Transcript identifiers

Ensembl transcripts: 12 — 7 protein_coding, 4 protein_coding_CDS_not_defined, 1 nonsense_mediated_decay

ENST00000290913, ENST00000503119, ENST00000504973, ENST00000508789, ENST00000510044, ENST00000513253, ENST00000514908, ENST00000515867, ENST00000870585, ENST00000870586, ENST00000921880, ENST00000921881

RefSeq mRNA: 2 — MANE Select: NM_032343 NM_001320610, NM_032343

CCDS: CCDS3041

Canonical transcript exons

ENST00000290913 — 8 exons

Exon	Start	End
ENSE00002077922	126704240	126704399
ENSE00003468511	126733078	126733222
ENSE00003474275	126852647	126852730
ENSE00003475348	126914680	126914750
ENSE00003501376	126730561	126730630
ENSE00003523464	126960196	126960420
ENSE00003572878	126957416	126957551
ENSE00003595543	126727078	126727186

Expression profiles

Bgee: expression breadth ubiquitous, 211 present calls, max score 94.76.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 12.8329 / max 108.1893, expressed in 1764 samples.

FANTOM5 promoters (9 alternative TSS)

Promoter ID	TPM avg	Samples expressed
38362	11.5035	1742
38366	0.7032	102
38367	0.2423	79
38368	0.0901	48
38365	0.0794	45
38369	0.0773	46
202916	0.0695	43
38371	0.0343	14
38370	0.0333	17

Top tissues by expression

249 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
left testis	UBERON:0004533	94.76	gold quality
right testis	UBERON:0004534	94.32	gold quality
cortical plate	UBERON:0005343	94.27	gold quality
prefrontal cortex	UBERON:0000451	94.12	gold quality
anterior cingulate cortex	UBERON:0009835	94.02	gold quality
right frontal lobe	UBERON:0002810	93.11	gold quality
Brodmann (1909) area 9	UBERON:0013540	92.86	gold quality
testis	UBERON:0000473	92.84	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	92.09	gold quality
amygdala	UBERON:0001876	91.58	gold quality
neocortex	UBERON:0001950	91.57	gold quality
frontal cortex	UBERON:0001870	91.53	gold quality
nucleus accumbens	UBERON:0001882	91.21	gold quality
hypothalamus	UBERON:0001898	90.67	gold quality
Ammon’s horn	UBERON:0001954	90.63	gold quality
cerebral cortex	UBERON:0000956	90.60	gold quality
sural nerve	UBERON:0015488	89.87	gold quality
caudate nucleus	UBERON:0001873	89.69	gold quality
forebrain	UBERON:0001890	89.20	gold quality
putamen	UBERON:0001874	89.19	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	88.86	gold quality
ganglionic eminence	UBERON:0004023	88.58	gold quality
brain	UBERON:0000955	88.26	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	87.98	gold quality
tibial nerve	UBERON:0001323	87.83	gold quality
right uterine tube	UBERON:0001302	87.61	gold quality
substantia nigra	UBERON:0002038	87.35	gold quality
spinal cord	UBERON:0002240	87.01	gold quality
temporal lobe	UBERON:0001871	86.99	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	86.43	gold quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 3.

Experiment	Marker?	Max mean expression
E-GEOD-135922	yes	25.32
E-MTAB-8142	yes	18.52
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

11 targeting CHCHD6, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-570-3P	99.96	72.41	4910
HSA-MIR-6716-5P	99.56	68.62	1244
HSA-MIR-1207-5P	99.49	69.11	2983
HSA-MIR-1324	99.46	66.57	1302
HSA-MIR-4685-5P	99.25	65.99	1563
HSA-MIR-6837-5P	99.25	65.47	1632
HSA-MIR-4763-3P	99.10	67.83	2649
HSA-MIR-5006-5P	98.79	66.92	1246
HSA-MIR-4686	98.77	66.87	964
HSA-MIR-5088-3P	98.29	66.63	1310
HSA-MIR-6890-3P	97.50	65.71	997

Literature-anchored findings (GeneRIF, showing 4)

• anti-mitofilin mAb immunocaptures its target protein in association with six other proteins, metaxins 1 and 2, SAM50, CHCHD3, CHCHD6 and DnaJC11, respectively (PMID:17624330)
• CHCM1/CHCHD6, novel mitochondrial protein linked to regulation of mitofilin and mitochondrial cristae morphology. (PMID:22228767)
• mitochondria exhibited lower cristae density in CHCHD6-knockout cells. CHCHD6 directly interacted with Sam50. (PMID:26530328)
• A CHCHD6-APP axis connects amyloid and mitochondrial pathology in Alzheimer’s disease. (PMID:36104602)

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	chchd6a	ENSDARG00000003206
danio_rerio	chchd6b	ENSDARG00000045978
mus_musculus	Chchd6	ENSMUSG00000030086
rattus_norvegicus	Chchd6	ENSRNOG00000060248
caenorhabditis_elegans		WBGENE00010942

Paralogs (1): CHCHD3 (ENSG00000106554)

Protein

Protein identifiers

MICOS complex subunit MIC25 — Q9BRQ6 (reviewed: Q9BRQ6)

Alternative names: Coiled-coil-helix cristae morphology protein 1, Coiled-coil-helix-coiled-coil-helix domain-containing protein 6

All UniProt accessions (4): Q9BRQ6, H0Y922, J3QTA6, J3QTB2

UniProt curated annotations — full annotation on UniProt →

Function. Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.

Subunit / interactions. Component of the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MICOS10/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and MICOS13/MIC13. This complex was also known under the names MINOS or MitOS complex. The MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2 (together described as components of the mitochondrial outer membrane sorting assembly machinery (SAM) complex) and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9. The MICOS and SAM complexes together with DNAJC11 are part of a large protein complex spanning both membranes termed the mitochondrial intermembrane space bridging (MIB) complex. Interacts with DISC1. Interacts with DISC1. Interacts with IMMT/MIC60. (Microbial infection) Interacts with human cytomegalovirus protein UL37 isoform vMIA; this interaction rewires mitochondria by engaging the conserved MICOS complex.

Subcellular location. Mitochondrion inner membrane. Mitochondrion.

Induction. Down-regulated following genotoxic stress.

Similarity. Belongs to the MICOS complex subunit Mic19 family. Metazoan Mic25 subfamily.

RefSeq proteins (2): NP_001307539, NP_115719* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR007964	MIC19/MIC25	Family
IPR042860	MIC25	Family

Pfam: PF05300

UniProt features (14 total): disulfide bond 2, region of interest 2, short sequence motif 2, modified residue 2, initiator methionine 1, chain 1, lipid moiety-binding region 1, sequence variant 1, domain 1, coiled-coil region 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9BRQ6-F1	80.29	0.50

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (3): 2, 13, 31

Disulfide bonds (2): 197–228, 207–218

Function

Pathways and Gene Ontology

Reactome pathways

3 pathways

ID	Pathway
R-HSA-8949613	Cristae formation
R-HSA-1592230	Mitochondrial biogenesis
R-HSA-1852241	Organelle biogenesis and maintenance

MSigDB gene sets: 94 (showing top): GSE18804_BRAIN_VS_COLON_TUMORAL_MACROPHAGE_UP, GOBP_CRISTAE_FORMATION, GOBP_INNER_MITOCHONDRIAL_MEMBRANE_ORGANIZATION, MODULE_256, MODULE_480, GOBP_DNA_DAMAGE_RESPONSE, GOCC_MITOCHONDRIAL_ENVELOPE, chr3q21, GOBP_MEMBRANE_ORGANIZATION, MODULE_333, MODULE_427, BOYLAN_MULTIPLE_MYELOMA_C_D_UP, GOCC_ORGANELLE_MEMBRANE_CONTACT_SITE, GOCC_OUTER_MITOCHONDRIAL_MEMBRANE_PROTEIN_COMPLEX, GOCC_ORGANELLE_INNER_MEMBRANE

GO Biological Process (3): DNA damage response (GO:0006974), inner mitochondrial membrane organization (GO:0007007), cristae formation (GO:0042407)

GO Molecular Function (1): protein binding (GO:0005515)

GO Cellular Component (8): SAM complex (GO:0001401), mitochondrion (GO:0005739), mitochondrial inner membrane (GO:0005743), cytosol (GO:0005829), mitochondrial crista junction (GO:0044284), MICOS complex (GO:0061617), MIB complex (GO:0140275), membrane (GO:0016020)

Reactome top-level categories

Rollup of top-2 pathways:

Category	Pathways
Mitochondrial biogenesis	1
Organelle biogenesis and maintenance	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cytoplasm	2
cellular anatomical structure	2
inner mitochondrial membrane protein complex	2
cellular response to stress	1
mitochondrial membrane organization	1
inner mitochondrial membrane organization	1
binding	1
mitochondrial outer membrane translocase complex	1
intracellular membrane-bounded organelle	1
organelle inner membrane	1
mitochondrial membrane	1
mitochondrial inner membrane	1
organelle membrane contact site	1

Protein interactions and networks

STRING

688 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
CHCHD6	IMMT	Q16891	994
CHCHD6	APOOL	Q6UXV4	993
CHCHD6	APOO	Q9BUR5	993
CHCHD6	CHCHD3	Q9NX63	983
CHCHD6	MICOS10	Q5TGZ0	982
CHCHD6	MICOS13	Q5XKP0	982
CHCHD6	CHCHD5	Q9BSY4	830
CHCHD6	MTX1	Q13505	827
CHCHD6	SAMM50	Q9Y512	822
CHCHD6	MTX2	O75431	770
CHCHD6	CHCHD10	Q8WYQ3	757
CHCHD6	DNAJC11	Q9NVH1	705
CHCHD6	MTX3	Q5HYI7	513
CHCHD6	TIMM22	Q9Y584	462
CHCHD6	TOMM40	O96008	451

IntAct

102 interactions, top by confidence:

A	B	Type	Score
IMMT	MTX2	psi-mi:“MI:0914”(association)	0.730
CTDSP1	CTDSPL	psi-mi:“MI:0914”(association)	0.640
NIPAL1	ESYT2	psi-mi:“MI:0914”(association)	0.640
SLC1A1	AGPAT2	psi-mi:“MI:0914”(association)	0.640
CHCHD10	IMMT	psi-mi:“MI:0915”(physical association)	0.600
CHCHD3	CHCHD6	psi-mi:“MI:0915”(physical association)	0.560
CHCHD6	SAMM50	psi-mi:“MI:0915”(physical association)	0.560
CLEC5A	TSPAN6	psi-mi:“MI:0914”(association)	0.530
APOOL	MTX2	psi-mi:“MI:0914”(association)	0.530
APLNR	METTL15	psi-mi:“MI:0914”(association)	0.530
SLC22A16	APBA3	psi-mi:“MI:0914”(association)	0.530
TRAK2	OGT	psi-mi:“MI:0914”(association)	0.530
TRAK1	MTX2	psi-mi:“MI:0914”(association)	0.530
CHCHD6	CHCHD10	psi-mi:“MI:2364”(proximity)	0.470
CHCHD6	PPP1CA	psi-mi:“MI:0407”(direct interaction)	0.440
HTRA2	HAX1	psi-mi:“MI:2364”(proximity)	0.420
Erh	BCLAF3	psi-mi:“MI:0915”(physical association)	0.400
CHCHD6	ZER1	psi-mi:“MI:0915”(physical association)	0.400
Bmpr1a	PLEKHG3	psi-mi:“MI:0914”(association)	0.350
Tnpo1	CCHCR1	psi-mi:“MI:0914”(association)	0.350
Rmdn3	DERL1	psi-mi:“MI:0914”(association)	0.350
RAB7A		psi-mi:“MI:0914”(association)	0.350
Aifm1	HMGB1	psi-mi:“MI:0914”(association)	0.350
MTX2		psi-mi:“MI:0914”(association)	0.350
Mtx2	NRDC	psi-mi:“MI:0914”(association)	0.350
Tmed2		psi-mi:“MI:0914”(association)	0.350
VAPA		psi-mi:“MI:0914”(association)	0.350

BioGRID (109): CHCHD6 (Affinity Capture-MS), CHCHD2 (Co-fractionation), NDUFA8 (Co-fractionation), CHCHD6 (Affinity Capture-MS), CHCHD6 (Affinity Capture-MS), CHCHD6 (Affinity Capture-MS), CHCHD6 (Affinity Capture-MS), CHCHD6 (Affinity Capture-MS), CHCHD6 (Affinity Capture-MS), CHCHD6 (Affinity Capture-MS), CHCHD6 (Affinity Capture-MS), CHCHD6 (Affinity Capture-MS), CHCHD6 (Affinity Capture-MS), CHCHD6 (Affinity Capture-MS), CHCHD6 (Affinity Capture-MS)

ESM2 similar proteins: A1A4P9, A4QNG1, B1WB06, B8JLV7, F4HVW5, F4IRM4, F4J264, F4JTI1, K7TQE3, O48781, O65573, O80462, O81893, O82387, Q0IY07, Q0WPN7, Q3EBL9, Q3KPR1, Q3ZBK3, Q5BK13, Q5M7V7, Q5M856, Q5MK23, Q5MK24, Q5RHZ2, Q6BDI9, Q6E7H0, Q7X6P3, Q7ZYB4, Q84JN1, Q84M47, Q84WF5, Q8IYF3, Q8L7N4, Q8LBH4, Q8RXH2, Q8S9J3, Q8W032, Q8W4F0, Q94BM7

Diamond homologs: D4A7N1, Q32L35, Q5FVV3, Q5U509, Q63ZW2, Q6AX41, Q91VN4, Q9BRQ6, Q9CRB9, Q5E9D3, Q9NX63

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 121 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Cristae formation	6	30.5×	1e-05
Mitochondrial biogenesis	7	17.3×	2e-05
Organelle biogenesis and maintenance	7	6.8×	6e-03

GO biological processes:

GO term	Partners	Fold	FDR
cristae formation	6	60.2×	2e-07
inner mitochondrial membrane organization	7	56.2×	2e-08

Disease & clinical

Clinical variants and AI predictions

ClinVar

52 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	39
Likely benign	6
Benign	1

Top pathogenic / likely-pathogenic (0)

SpliceAI

2803 predictions. Top by Δscore:

Variant	Effect	Δscore
3:126704367:G:GT	donor_gain	1.0000
3:126704398:GG:G	donor_gain	1.0000
3:126704399:GG:G	donor_gain	1.0000
3:126727067:T:TA	acceptor_gain	1.0000
3:126727073:CTTA:C	acceptor_loss	1.0000
3:126727075:TAGCT:T	acceptor_loss	1.0000
3:126727076:A:AG	acceptor_gain	1.0000
3:126727076:AG:A	acceptor_loss	1.0000
3:126727076:AGCT:A	acceptor_gain	1.0000
3:126727077:G:GT	acceptor_gain	1.0000
3:126727077:GC:G	acceptor_gain	1.0000
3:126727077:GCT:G	acceptor_gain	1.0000
3:126727077:GCTG:G	acceptor_gain	1.0000
3:126727077:GCTGT:G	acceptor_gain	1.0000
3:126727182:CAAAG:C	donor_loss	1.0000
3:126727183:AAAGG:A	donor_loss	1.0000
3:126727184:AAGGT:A	donor_loss	1.0000
3:126727187:G:GA	donor_loss	1.0000
3:126727188:T:G	donor_loss	1.0000
3:126730559:A:AG	acceptor_gain	1.0000
3:126730560:G:GA	acceptor_gain	1.0000
3:126730628:GAG:G	donor_gain	1.0000
3:126733074:A:AG	acceptor_gain	1.0000
3:126733074:ACAG:A	acceptor_gain	1.0000
3:126733075:C:G	acceptor_gain	1.0000
3:126733075:CA:C	acceptor_loss	1.0000
3:126733076:A:AC	acceptor_loss	1.0000
3:126733076:AG:A	acceptor_gain	1.0000
3:126733077:GG:G	acceptor_gain	1.0000
3:126733077:GGT:G	acceptor_gain	1.0000

AlphaMissense

1527 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
3:126957522:T:G	Y225D	0.992
3:126727103:T:C	M38T	0.991
3:126704352:T:C	F14L	0.990
3:126704354:C:A	F14L	0.990
3:126704354:C:G	F14L	0.990
3:126957501:T:A	C218S	0.990
3:126957502:G:C	C218S	0.990
3:126957438:T:C	C197R	0.987
3:126957438:T:A	C197S	0.986
3:126957439:G:C	C197S	0.986
3:126957501:T:C	C218R	0.986
3:126957460:T:C	I204T	0.984
3:126727079:T:A	L30Q	0.983
3:126914722:G:C	A180P	0.982
3:126957503:C:G	C218W	0.982
3:126704391:G:C	G27R	0.981
3:126957440:C:G	C197W	0.981
3:126957439:G:A	C197Y	0.980
3:126957502:G:A	C218Y	0.980
3:126957522:T:A	Y225N	0.980
3:126914713:T:C	F177L	0.979
3:126914714:T:C	F177S	0.979
3:126914715:C:A	F177L	0.979
3:126914715:C:G	F177L	0.979
3:126957460:T:G	I204S	0.978
3:126957468:T:A	C207S	0.977
3:126957468:T:C	C207R	0.977
3:126957469:G:C	C207S	0.977
3:126914725:G:C	A181P	0.975
3:126957470:C:G	C207W	0.975

dbSNP variants (sampled 300 via entrez): RS1000020815 (3:126938955 T>C), RS1000030632 (3:126814623 A>G), RS1000047658 (3:126914761 G>A,T), RS1000070554 (3:126940752 C>T), RS1000090400 (3:126741248 G>A,T), RS1000094687 (3:126787720 T>A), RS1000103595 (3:126768463 A>AT), RS1000118480 (3:126934018 C>CT), RS1000121440 (3:126741055 A>C,T), RS1000122579 (3:126952357 C>T), RS1000128477 (3:126891895 G>A), RS1000137788 (3:126768175 A>G,T), RS1000163286 (3:126738173 G>T), RS1000189835 (3:126909140 G>A,T), RS1000208348 (3:126801955 G>A,C)

Disease associations

OMIM: gene MIM:615634 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

4 associations (top):

Study	Trait	p-value
GCST004860_115	Alcoholic chronic pancreatitis	4.000000e-06
GCST004860_138	Alcoholic chronic pancreatitis	2.000000e-06
GCST008545_2	Diabetic maculopathy with decreased visual acuity in type 2 diabetes	7.000000e-07
GCST011011_27	Youthful appearance (self-reported)	1.000000e-51

EFO canonical traits (2, from GWAS)

EFO ID	Trait name
EFO:0008385	visual acuity measurement
EFO:0010133	diabetic maculopathy

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL4105993 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
7.24	Kd	57.61	nM	CHEMBL5653589
7.24	ED50	57.61	nM	CHEMBL5653589

PubChem BioAssay actives

1 with measured affinity, of 4 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2148066: Binding affinity to human CHCHD6 incubated for 45 mins by Kinobead based pull down assay	kd	0.0576	uM

CTD chemical–gene interactions

30 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Benzo(a)pyrene	affects methylation, decreases expression, decreases methylation	4
Cisplatin	affects cotreatment, decreases expression	3
Valproic Acid	affects expression, decreases methylation	2
aristolochic acid I	decreases expression	1
alpha-pinene	increases oxidation, increases abundance, affects cotreatment	1
bisphenol A	affects cotreatment, decreases methylation	1
mono-(2-ethylhexyl)phthalate	decreases expression	1
sodium arsenite	decreases expression	1
benzo(e)pyrene	increases methylation	1
aflatoxin B2	increases methylation	1
methacrylaldehyde	affects cotreatment, increases oxidation, increases abundance	1
CGP 52608	affects binding, increases reaction	1
jinfukang	affects cotreatment, decreases expression	1
Temozolomide	decreases expression	1
Pioglitazone	decreases expression	1
Fulvestrant	decreases methylation, affects cotreatment	1
Acrolein	affects cotreatment, increases oxidation, increases abundance	1
Air Pollutants	increases abundance, increases oxidation, affects cotreatment	1
Hydrogen Peroxide	affects expression	1
Lead	affects expression	1
Methapyrilene	increases methylation	1
Mustard Gas	decreases expression	1
Ozone	affects cotreatment, increases oxidation, increases abundance	1
Tobacco Smoke Pollution	decreases expression	1
Urethane	decreases expression	1
Cyclosporine	increases expression	1
Aflatoxin B1	increases methylation	1
Okadaic Acid	decreases expression	1
beta-Naphthoflavone	decreases expression	1
Volatile Organic Compounds	affects cotreatment, increases oxidation	1

ChEMBL screening assays

2 unique, capped per target: 2 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL4012555	Binding	Binding affinity to MICOS complex subunit MIC25 in human INA-6 cells after 3 hrs by nanoLC-MS/MS method	Ugi Reaction-Derived α-Acyl Aminocarboxamides Bind to Phosphatidylinositol 3-Kinase-Related Kinases, Inhibit HSF1-Dependent Heat Shock Response, and Induce Apoptosis in Multiple Myeloma Cells. — J Med Chem

Cellosaurus cell lines

3 cell lines: 2 cancer cell line, 1 transformed cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B2UA	Abcam HEK293T CHCHD6 KO	Transformed cell line	Female
CVCL_SI83	HAP1 CHCHD6 (-) 1	Cancer cell line	Male
CVCL_SI84	HAP1 CHCHD6 (-) 2	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.