CRISP2

Summary

CRISP2 (cysteine rich secretory protein 2, HGNC:12024) is a protein-coding gene on chromosome 6p12.3, encoding Cysteine-rich secretory protein 2 (P16562). May regulate some ion channels’ activity and thereby regulate calcium fluxes during sperm capacitation.

Predicted to be involved in sexual reproduction. Predicted to be located in extracellular region. Predicted to be active in extracellular space.

Source: NCBI Gene 7180 — RefSeq curated summary.

At a glance

• GWAS associations: 2
• Clinical variants (ClinVar): 39 total
• MANE Select transcript: NM_003296

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:12024
Approved symbol	CRISP2
Name	cysteine rich secretory protein 2
Location	6p12.3
Locus type	gene with protein product
Status	Approved
Aliases	CRISP-2, CT36
Ensembl gene	ENSG00000124490
Ensembl biotype	protein_coding
OMIM	187430
Entrez	7180

Gene structure

Transcript identifiers

Ensembl transcripts: 8 — 8 protein_coding

ENST00000339139, ENST00000616725, ENST00000618917, ENST00000856936, ENST00000856937, ENST00000856938, ENST00000856939, ENST00000856940

RefSeq mRNA: 6 — MANE Select: NM_003296 NM_001142407, NM_001142408, NM_001142417, NM_001142435, NM_001261822, NM_003296

CCDS: CCDS4928

Canonical transcript exons

ENST00000339139 — 10 exons

Exon	Start	End
ENSE00001138788	49695836	49695924
ENSE00001138795	49697860	49697957
ENSE00001138811	49699804	49699891
ENSE00001368027	49713475	49713543
ENSE00001375729	49692359	49692900
ENSE00001378296	49712501	49712604
ENSE00001381380	49711285	49711321
ENSE00001383687	49709131	49709205
ENSE00002444967	49698362	49698507
ENSE00002472351	49700668	49700784

Expression profiles

Bgee: expression breadth ubiquitous, 163 present calls, max score 99.99.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 0.9553 / max 714.0675, expressed in 39 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
73917	0.7336	29
73918	0.2217	19

Top tissues by expression

281 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
sperm	CL:0000019	99.99	gold quality
male germ cell	CL:0000015	99.76	gold quality
adult organism	UBERON:0007023	99.25	gold quality
left testis	UBERON:0004533	99.05	gold quality
right testis	UBERON:0004534	98.98	gold quality
right uterine tube	UBERON:0001302	97.76	gold quality
testis	UBERON:0000473	96.26	gold quality
pancreatic ductal cell	CL:0002079	92.48	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	91.09	gold quality
bronchial epithelial cell	CL:0002328	90.74	gold quality
epithelium of bronchus	UBERON:0002031	89.81	gold quality
bronchus	UBERON:0002185	88.94	gold quality
lower esophagus mucosa	UBERON:0035834	87.99	gold quality
epithelial cell of pancreas	CL:0000083	83.98	silver quality
minor salivary gland	UBERON:0001830	79.78	gold quality
caput epididymis	UBERON:0004358	79.62	gold quality
gall bladder	UBERON:0002110	79.61	gold quality
saliva-secreting gland	UBERON:0001044	79.00	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	78.75	gold quality
bone marrow	UBERON:0002371	75.99	gold quality
mouth mucosa	UBERON:0003729	75.52	gold quality
cauda epididymis	UBERON:0004360	74.57	gold quality
trabecular bone tissue	UBERON:0002483	74.53	gold quality
seminal vesicle	UBERON:0000998	73.69	gold quality
fallopian tube	UBERON:0003889	72.85	gold quality
oviduct epithelium	UBERON:0004804	71.70	gold quality
nasal cavity mucosa	UBERON:0001826	71.36	gold quality
choroid plexus epithelium	UBERON:0003911	70.39	gold quality
corpus epididymis	UBERON:0004359	69.67	gold quality
parotid gland	UBERON:0001831	68.90	silver quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 2.

Experiment	Marker?	Max mean expression
E-GEOD-134144	yes	12207.97
E-HCAD-38	yes	5996.75
E-ANND-3	no	3.24

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

38 targeting CRISP2, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-6870-5P	99.99	68.55	2115
HSA-LET-7F-2-3P	99.98	70.98	2588
HSA-MIR-1185-1-3P	99.98	71.04	2593
HSA-MIR-1185-2-3P	99.98	71.04	2593
HSA-MIR-27A-3P	99.98	72.13	2955
HSA-MIR-27B-3P	99.98	72.13	2955
HSA-MIR-9985	99.98	72.11	2939
HSA-MIR-4723-5P	99.97	68.70	2034
HSA-MIR-5698	99.97	68.49	2029
HSA-MIR-7111-5P	99.97	68.48	2062
HSA-MIR-548AJ-3P	99.96	73.38	5345
HSA-MIR-548X-3P	99.96	73.38	5345
HSA-MIR-767-5P	99.95	70.85	993
HSA-MIR-548J-3P	99.94	72.61	4881
HSA-MIR-6835-3P	99.93	70.49	2904
HSA-MIR-4778-3P	99.93	70.40	1818
HSA-MIR-548AE-3P	99.93	72.66	4867
HSA-MIR-548AH-3P	99.93	72.54	4872
HSA-MIR-548AM-3P	99.93	72.54	4872
HSA-MIR-548AQ-3P	99.93	72.66	4867
HSA-MIR-8062	99.88	68.43	995
HSA-MIR-582-5P	99.47	70.79	2635
HSA-MIR-501-3P	99.33	66.12	651
HSA-MIR-502-3P	99.33	66.12	651
HSA-MIR-491-3P	98.88	68.86	1224
HSA-MIR-487A-5P	98.85	69.37	993
HSA-MIR-487B-5P	98.85	69.48	987
HSA-MIR-7155-5P	98.65	66.14	1290
HSA-MIR-4700-5P	98.63	67.43	1915
HSA-MIR-4317	98.49	67.09	987

Literature-anchored findings (GeneRIF, showing 15)

• TPX1 is a component of the sperm acrosome that remains associated with sperm after capacitation and acrosome reaction , and is relevant for sperm-oocyte interaction. (PMID:15734896)
• The protein level and localization of TPX1 were altered in patients with spermatogenic arrest and in infertile men with oligoasthenoteratospermia syndrome. (PMID:16759931)
• Polymorphisms within the CRISP2 gene have no significant association with male infertility. (PMID:18550510)
• Additional transcription changes likely associated with Th2-like eosinophilic inflammation were prominent and included decreased CRISP2&3. (PMID:20625511)
• The down-regulation of CRISP2 mRNA and protein expressions in the sperm of asthenospermia patients may be closely related with decreased sperm motility. (PMID:21485539)
• CRISP2 rescues the phenotype of yeast mutants lacking Pry function and purified CRISP2 binds cholesterol in vitro, indicating that lipid binding is a conserved function of the CAP superfamily proteins. (PMID:23027975)
• Study shows the re-association of sperm acrosomal CRISP2 at the EqS during the acrosome reaction. Data showed that F-actin is not involved in the relocalization of CRISP2. Found that basic,but not acidic, conditions can solubilize CRISP2 from sperm cells. (PMID:23661501)
• Preliminary insight into the mechanism leading to the reduced CRISP2 expression in asthenozoospermia, offering a potential therapeutic target for treating male infertility or for male contraception. (PMID:25505194)
• Crisp2 genetic defects in sperm from humans and mice result in male infertility. (PMID:26786179)
• Results identified CRISP2 as a potential binding protein of PSP94 from human sperm. (PMID:27161017)
• low expression of CRISP2 protein rather than its mRNA was observed in the ejaculated spermatozoa from asthenoteratozoospermic patients as compared with normozoospermic males (PMID:27517483)
• The integrated network analysis identified BRCA1 and CRISP2 differentially methylated regions (DMR) as the most central disease-associated DNA methylation biomarkers. Moreover, methylation changes at BRCA1 and CRISP2 specific CpG sites were consistently associated with subclinical atherosclerosis measures. (PMID:28698603)
• DNA methylation profiling identifies epigenetic differences between early versus late stages of diabetic chronic kidney disease. (PMID:33146725)
• Discovery and validation of novel biomarkers for detection of cervical cancer. (PMID:33624385)
• CRISP2, CATSPER1 and PATE1 Expression in Human Asthenozoospermic Semen. (PMID:34440724)

Cross-species orthologs

46 orthologs

Organism	Symbol	Gene ID
mus_musculus	Crisp2	ENSMUSG00000023930
rattus_norvegicus	Crisp2	ENSRNOG00000013225
drosophila_melanogaster	Ag5r	FBGN0015010
drosophila_melanogaster	Ag5r2	FBGN0020508
drosophila_melanogaster	CG9400	FBGN0030562
drosophila_melanogaster	CG10651	FBGN0032853
drosophila_melanogaster	CG9822	FBGN0034623
drosophila_melanogaster	CG17974	FBGN0034624
drosophila_melanogaster	CG3640	FBGN0035042
drosophila_melanogaster	CG8072	FBGN0036070
drosophila_melanogaster	CG6628	FBGN0036072
drosophila_melanogaster	scpr-C	FBGN0037879
drosophila_melanogaster	scpr-B	FBGN0037888
drosophila_melanogaster	scpr-A	FBGN0037889
drosophila_melanogaster	CG8483	FBGN0038126
drosophila_melanogaster	CG30486	FBGN0050486
drosophila_melanogaster	antr	FBGN0050488
drosophila_melanogaster	CG31286	FBGN0051286
drosophila_melanogaster	CG32313	FBGN0052313
drosophila_melanogaster	CG32679	FBGN0052679
drosophila_melanogaster	CG34002	FBGN0054002
drosophila_melanogaster	CG17575	FBGN0250842
drosophila_melanogaster	CG42564	FBGN0260766
drosophila_melanogaster	CG42780	FBGN0261848
drosophila_melanogaster	CG43775	FBGN0264297
drosophila_melanogaster	CG43776	FBGN0264298
drosophila_melanogaster	CG43777	FBGN0264299
caenorhabditis_elegans		WBGENE00004742
caenorhabditis_elegans		WBGENE00007397
caenorhabditis_elegans		WBGENE00008027
caenorhabditis_elegans		WBGENE00008028
caenorhabditis_elegans		WBGENE00008029
caenorhabditis_elegans		WBGENE00008030
caenorhabditis_elegans		WBGENE00008625
caenorhabditis_elegans		WBGENE00009891
caenorhabditis_elegans		WBGENE00009895
caenorhabditis_elegans		WBGENE00009896
caenorhabditis_elegans		WBGENE00012816
caenorhabditis_elegans		WBGENE00013971
caenorhabditis_elegans		WBGENE00013972
caenorhabditis_elegans		WBGENE00015246
caenorhabditis_elegans		WBGENE00017055
caenorhabditis_elegans		WBGENE00017183
caenorhabditis_elegans		WBGENE00019178
caenorhabditis_elegans		WBGENE00019179
caenorhabditis_elegans		WBGENE00021780

Paralogs (13): CRISP3 (ENSG00000096006), R3HDML (ENSG00000101074), CRISPLD2 (ENSG00000103196), CRISPLD1 (ENSG00000121005), GLIPR2 (ENSG00000122694), CRISP1 (ENSG00000124812), PI15 (ENSG00000137558), GLIPR1 (ENSG00000139278), CLEC18B (ENSG00000140839), CLEC18A (ENSG00000157322), CLEC18C (ENSG00000157335), GLIPR1L1 (ENSG00000173401), GLIPR1L2 (ENSG00000180481)

Protein

Protein identifiers

Cysteine-rich secretory protein 2 — P16562 (reviewed: P16562)

Alternative names: Cancer/testis antigen 36, Testis-specific protein TPX-1

All UniProt accessions (2): A0A024RD74, P16562

UniProt curated annotations — full annotation on UniProt →

Function. May regulate some ion channels’ activity and thereby regulate calcium fluxes during sperm capacitation.

Subunit / interactions. Interacts with NSUN4 isoform 3.

Subcellular location. Secreted.

Tissue specificity. Testis and epididymis.

Similarity. Belongs to the CRISP family.

Isoforms (2)

UniProt ID	Names	Canonical?
P16562-1	1	yes
P16562-2	2

RefSeq proteins (6): NP_001135879, NP_001135880, NP_001135889, NP_001135907, NP_001248751, NP_003287* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001283	CRISP-related	Family
IPR003582	ShKT_dom	Domain
IPR013871	Cysteine_rich_secretory	Domain
IPR014044	CAP_dom	Domain
IPR018244	Allrgn_V5/Tpx1_CS	Conserved_site
IPR034117	SCP_CRISP	Domain
IPR035940	CAP_sf	Homologous_superfamily
IPR042076	Crisp-like_dom	Homologous_superfamily

Pfam: PF00188, PF08562

UniProt features (18 total): disulfide bond 5, sequence conflict 2, strand 2, helix 2, domain 2, signal peptide 1, chain 1, sequence variant 1, turn 1, splice variant 1

Structure

Experimental structures (PDB)

1 structures.

PDB	Method	Resolution (Å)
2CQ7	SOLUTION NMR

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-P16562-F1	93.33	0.88

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Disulfide bonds (5): 189–196, 192–201, 205–238, 214–232, 223–236

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 76 (showing top): GSE45365_CD8A_DC_VS_CD11B_DC_IFNAR_KO_UP, ACEVEDO_LIVER_CANCER_WITH_H3K27ME3_UP, OSWALD_HEMATOPOIETIC_STEM_CELL_IN_COLLAGEN_GEL_UP, chr6p12, GNF2_CCNA1, MORF_EPHA7, MORF_RAB3A, MORF_BMPR2, MORF_DCC, WEBER_METHYLATED_HCP_IN_FIBROBLAST_DN, LEE_LIVER_CANCER_MYC_TGFA_DN, WEBER_METHYLATED_HCP_IN_SPERM_DN, YOSHIMURA_MAPK8_TARGETS_UP, BRUINS_UVC_RESPONSE_VIA_TP53_GROUP_B, BRUINS_UVC_RESPONSE_MIDDLE

GO Biological Process (0):

GO Molecular Function (0):

GO Cellular Component (2): obsolete extracellular space (GO:0005615), extracellular region (GO:0005576)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	1

Protein interactions and networks

STRING

1296 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
CRISP2	PGK2	P07205	813
CRISP2	CATSPER1	Q8NEC5	751
CRISP2	PLB1	Q6P1J6	655
CRISP2	IL4I1	Q96RQ9	653
CRISP2	LTA4H	P09960	589
CRISP2	GGNBP2	Q9H3C7	579
CRISP2	IZUMO1	Q8IYV9	563
CRISP2	PATE1	Q8WXA2	562
CRISP2	AKAP4	Q5JQC9	542
CRISP2	PRM2	P04554	528
CRISP2	TMEM37	Q8WXS4	527
CRISP2	PLA2G2A	P14555	515
CRISP2	IZUMO3	Q5VZ72	493
CRISP2	PLCZ1	Q86YW0	492
CRISP2	PKDREJ	Q9NTG1	491

IntAct

5 interactions, top by confidence:

A	B	Type	Score
CRISP2	TUBA4A	psi-mi:“MI:0914”(association)	0.530
CREB3	CRISP2	psi-mi:“MI:0915”(physical association)	0.370
CRISP2	HSPA5	psi-mi:“MI:0914”(association)	0.350
CRISP2	LRP5	psi-mi:“MI:0914”(association)	0.350

BioGRID (51): TACC3 (Affinity Capture-MS), CTSF (Affinity Capture-MS), CNTNAP3 (Affinity Capture-MS), NLGN2 (Affinity Capture-MS), TUBA4A (Affinity Capture-MS), CRISP2 (Affinity Capture-MS), HSPA5 (Affinity Capture-MS), TUBA4A (Affinity Capture-MS), NLGN2 (Affinity Capture-MS), TACC3 (Affinity Capture-MS), CNTNAP3 (Affinity Capture-MS), PLXNA1 (Affinity Capture-MS), PCSK6 (Affinity Capture-MS), CELSR1 (Affinity Capture-MS), SEMA3B (Affinity Capture-MS)

ESM2 similar proteins: A0A0D3QS98, A0A0D3QS99, A2A5I3, O19010, O19011, O42596, P01137, P04202, P04629, P07200, P09533, P16562, P17246, P18341, P35739, P50414, P54108, P54831, P57110, Q01974, Q38HS2, Q3KPV7, Q3UFB7, Q505J3, Q5R7Y0, Q5T4F7, Q60477, Q658N2, Q6UWX4, Q7T141, Q7TSQ1, Q80XH4, Q8BG58, Q91009, Q99JR5, Q9CXM0, Q9D2G9, Q9EQT5, Q9GZM7, Q9H3Y0

Diamond homologs: A0A182GL09, A0A1S4EWW7, A0A218QX58, A1BQQ5, A6MFK9, A6QLZ7, A8S6B6, A9QQ26, A9YME1, B2MVK7, B9URJ1, C0ITL3, D4B327, D4P2Y4, F8J2D4, G3CJR9, O19010, O43692, P0CB15, P0DMB9, P0DMT4, P0DPU0, P0DPU1, P0DPU2, P0DPU5, P0DPV2, P0DSI3, P10736, P10737, P12020, P16562, P16563, P35759, P35760, P35778, P35779, P35780, P35781, P35782, P35783

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

39 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	34
Likely benign	4
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1071 predictions. Top by Δscore:

Variant	Effect	Δscore
6:49695922:CCA:C	acceptor_gain	1.0000
6:49695923:CAC:C	acceptor_gain	1.0000
6:49695925:C:CC	acceptor_gain	1.0000
6:49698356:TCTTA:T	donor_loss	1.0000
6:49698357:CTTAC:C	donor_loss	1.0000
6:49698358:TTACC:T	donor_loss	1.0000
6:49698359:TACC:T	donor_loss	1.0000
6:49698360:A:AT	donor_loss	1.0000
6:49698506:ACCT:A	acceptor_loss	1.0000
6:49698508:CTA:C	acceptor_loss	1.0000
6:49698509:T:C	acceptor_loss	1.0000
6:49699799:CATA:C	donor_gain	1.0000
6:49699802:A:AC	donor_gain	1.0000
6:49699803:C:CC	donor_gain	1.0000
6:49700004:C:CT	donor_gain	1.0000
6:49700005:T:TT	donor_gain	1.0000
6:49700652:CAGCA:C	donor_gain	1.0000
6:49700667:CCAT:C	donor_gain	1.0000
6:49700783:TC:T	acceptor_gain	1.0000
6:49700784:CC:C	acceptor_gain	1.0000
6:49700784:CCTAA:C	acceptor_loss	1.0000
6:49700785:C:CC	acceptor_gain	1.0000
6:49700785:CTA:C	acceptor_loss	1.0000
6:49700786:T:A	acceptor_loss	1.0000
6:49712499:A:AC	donor_gain	1.0000
6:49712500:C:CA	donor_gain	1.0000
6:49712500:CT:C	donor_gain	1.0000
6:49712500:CTA:C	donor_gain	1.0000
6:49712500:CTAA:C	donor_gain	1.0000
6:49712500:CTAAT:C	donor_gain	1.0000

AlphaMissense

1600 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
6:49692810:C:G	C232S	0.996
6:49692811:A:T	C232S	0.996
6:49699886:C:A	W63C	0.996
6:49699886:C:G	W63C	0.996
6:49700707:C:A	R48S	0.996
6:49700707:C:G	R48S	0.996
6:49700708:C:G	R48T	0.996
6:49697926:C:G	C150S	0.995
6:49697927:A:T	C150S	0.995
6:49698416:A:C	F121L	0.995
6:49698416:A:T	F121L	0.995
6:49698418:A:G	F121L	0.995
6:49698458:C:A	W107C	0.995
6:49698458:C:G	W107C	0.995
6:49699842:C:G	C78S	0.995
6:49699843:A:T	C78S	0.995
6:49700708:C:A	R48M	0.995
6:49692864:C:G	C214S	0.994
6:49692865:A:T	C214S	0.994
6:49695838:C:G	C201S	0.994
6:49695839:A:T	C201S	0.994
6:49697875:C:T	C167Y	0.994
6:49697876:A:G	C167R	0.994
6:49697926:C:T	C150Y	0.994
6:49698460:A:G	W107R	0.994
6:49698460:A:T	W107R	0.994
6:49698488:A:C	N97K	0.994
6:49698488:A:T	N97K	0.994
6:49697874:G:C	C167W	0.993
6:49697875:C:G	C167S	0.993

dbSNP variants (sampled 300 via entrez): RS1000005963 (6:49690796 T>C), RS1000032616 (6:49695432 C>T), RS1000067331 (6:49690130 A>G), RS1000128221 (6:49696358 A>G), RS1000220330 (6:49715263 T>C), RS1000251738 (6:49696501 G>T), RS1000384550 (6:49709384 C>T), RS1000389362 (6:49679272 C>A), RS1000420013 (6:49690317 C>A,T), RS1000435915 (6:49713293 G>A), RS1000682289 (6:49700974 T>C), RS1000709541 (6:49714882 C>A,G,T), RS1000726978 (6:49708036 A>G), RS1000860059 (6:49684613 T>G), RS1000879091 (6:49714805 T>C)

Disease associations

OMIM: gene MIM:187430 | disease phenotypes:

GenCC curated gene-disease

Mondo (1): megacolon (MONDO:0001273)

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

2 associations (top):

Study	Trait	p-value
GCST003102_6	Bone mineral density (hip) and age at menarche	9.000000e-06
GCST006585_1873	Blood protein levels	5.000000e-107

EFO canonical traits (2, from GWAS)

EFO ID	Trait name
EFO:0004703	age at menarche
EFO:0007702	hip bone mineral density

MeSH disease descriptors (1)

Descriptor	Name	Tree numbers
D008531	Megacolon	C06.405.469.158.701

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

14 total (human), top 14 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
arsenite	increases methylation	1
pentanal	increases expression	1
PKF115-584	affects expression	1
Decitabine	increases expression	1
Arsenic	affects methylation	1
Atrazine	increases expression	1
Benzo(a)pyrene	affects methylation	1
Cadmium	decreases expression, increases abundance	1
Doxorubicin	decreases expression	1
Tobacco Smoke Pollution	decreases expression	1
Valproic Acid	decreases methylation	1
Aflatoxin B1	decreases methylation	1
Lithium Chloride	decreases expression	1
Cadmium Chloride	decreases expression, increases abundance	1

Clinical trials (associated diseases)

2 trials via MONDO — disease-level, not drug-specific.

Trial	Phase	Status	Title
NCT04340856	Not specified	COMPLETED	Retrospective, Uncontrolled Cohort Study on the Therapy of Chronic Megalon
NCT07470892	Not specified	NOT_YET_RECRUITING	Preoperative Fish Oil PN and Prognosis After Constipation Surgery