CROT

Summary

CROT (carnitine O-octanoyltransferase, HGNC:2366) is a protein-coding gene on chromosome 7q21.12, encoding Peroxisomal carnitine O-octanoyltransferase (Q9UKG9). Beta-oxidation of fatty acids.

This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.

Source: NCBI Gene 54677 — RefSeq curated summary.

At a glance

• GWAS associations: 2
• Clinical variants (ClinVar): 39 total
• Druggable target: yes
• MANE Select transcript: NM_021151

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:2366
Approved symbol	CROT
Name	carnitine O-octanoyltransferase
Location	7q21.12
Locus type	gene with protein product
Status	Approved
Aliases	COT
Ensembl gene	ENSG00000005469
Ensembl biotype	protein_coding
OMIM	606090
Entrez	54677

Gene structure

Transcript identifiers

Ensembl transcripts: 11 — 8 protein_coding, 3 retained_intron

ENST00000331536, ENST00000412227, ENST00000419147, ENST00000442291, ENST00000469785, ENST00000479014, ENST00000488850, ENST00000881399, ENST00000881400, ENST00000881401, ENST00000947266

RefSeq mRNA: 3 — MANE Select: NM_021151 NM_001143935, NM_001243745, NM_021151

CCDS: CCDS47634, CCDS5604, CCDS59062

Canonical transcript exons

ENST00000331536 — 18 exons

Exon	Start	End
ENSE00000703802	87392948	87393067
ENSE00000703806	87392730	87392823
ENSE00000703810	87392566	87392644
ENSE00000703814	87391589	87391712
ENSE00000703817	87382413	87382543
ENSE00000703819	87382074	87382181
ENSE00000703829	87375828	87375953
ENSE00000703832	87375632	87375725
ENSE00000703835	87369376	87369484
ENSE00000703841	87361390	87361571
ENSE00000703844	87359206	87359330
ENSE00000877143	87349048	87349183
ENSE00001016681	87346340	87346430
ENSE00001318633	87398524	87399794
ENSE00001913179	87345664	87345767
ENSE00003472071	87381910	87381993
ENSE00003526250	87361728	87361852
ENSE00003682267	87377349	87377450

Expression profiles

Bgee: expression breadth ubiquitous, 287 present calls, max score 95.66.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 6.9320 / max 185.4348, expressed in 1642 samples.

FANTOM5 promoters (4 alternative TSS)

Promoter ID	TPM avg	Samples expressed
79393	3.8449	1526
79394	1.8295	878
79395	0.8436	387
79396	0.4140	192

Top tissues by expression

295 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
esophagus squamous epithelium	UBERON:0006920	95.66	gold quality
cervix squamous epithelium	UBERON:0006922	95.35	gold quality
seminal vesicle	UBERON:0000998	95.33	gold quality
jejunal mucosa	UBERON:0000399	95.27	gold quality
mucosa of urinary bladder	UBERON:0001259	94.79	gold quality
epithelium of esophagus	UBERON:0001976	94.74	gold quality
squamous epithelium	UBERON:0006914	94.73	gold quality
upper leg skin	UBERON:0004262	94.22	gold quality
duodenum	UBERON:0002114	93.35	gold quality
tongue squamous epithelium	UBERON:0006919	92.88	gold quality
palpebral conjunctiva	UBERON:0001812	92.78	gold quality
urethra	UBERON:0000057	92.73	gold quality
hair follicle	UBERON:0002073	92.52	gold quality
gingival epithelium	UBERON:0001949	92.15	gold quality
cervix epithelium	UBERON:0004801	92.01	gold quality
gingiva	UBERON:0001828	91.78	gold quality
epithelium of nasopharynx	UBERON:0001951	91.43	gold quality
left lobe of thyroid gland	UBERON:0001120	91.36	gold quality
right lobe of thyroid gland	UBERON:0001119	91.35	gold quality
germinal epithelium of ovary	UBERON:0001304	91.26	gold quality
jejunum	UBERON:0002115	91.26	gold quality
choroid plexus epithelium	UBERON:0003911	91.19	gold quality
mammalian vulva	UBERON:0000997	91.13	gold quality
thyroid gland	UBERON:0002046	90.89	gold quality
corpus epididymis	UBERON:0004359	90.31	gold quality
cauda epididymis	UBERON:0004360	90.19	gold quality
oral cavity	UBERON:0000167	90.16	gold quality
skin of hip	UBERON:0001554	89.83	gold quality
urinary bladder	UBERON:0001255	89.73	gold quality
parietal pleura	UBERON:0002400	89.73	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

79 targeting CROT, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-6867-5P	100.00	82.21	3464
HSA-MIR-6758-5P	100.00	66.21	1470
HSA-MIR-6856-5P	100.00	65.47	1298
HSA-MIR-33A-5P	99.99	68.62	1055
HSA-MIR-33B-5P	99.99	68.58	1062
HSA-MIR-513B-5P	99.99	69.96	2150
HSA-MIR-5696	99.98	72.36	4487
HSA-MIR-302E	99.96	70.74	2669
HSA-MIR-548AJ-3P	99.96	73.38	5345
HSA-MIR-548X-3P	99.96	73.38	5345
HSA-MIR-548J-3P	99.94	72.61	4881
HSA-MIR-548AE-3P	99.93	72.66	4867
HSA-MIR-548AH-3P	99.93	72.54	4872
HSA-MIR-548AM-3P	99.93	72.54	4872
HSA-MIR-548AQ-3P	99.93	72.66	4867
HSA-MIR-7-1-3P	99.91	71.53	4384
HSA-MIR-7-2-3P	99.91	71.40	4394
HSA-MIR-6809-3P	99.91	71.45	3814
HSA-MIR-106A-5P	99.90	73.94	2683
HSA-MIR-4753-3P	99.90	71.03	3786
HSA-MIR-302A-3P	99.89	71.23	1777
HSA-MIR-302B-3P	99.89	71.23	1777
HSA-MIR-302C-3P	99.89	71.20	1778
HSA-MIR-302D-3P	99.89	71.25	1777
HSA-MIR-17-5P	99.89	73.83	2665
HSA-MIR-153-5P	99.89	73.86	6317
HSA-MIR-106B-5P	99.88	74.72	2795
HSA-MIR-20A-5P	99.88	74.76	2769
HSA-MIR-526B-3P	99.88	74.06	2587
HSA-MIR-20B-5P	99.88	74.01	2621

Literature-anchored findings (GeneRIF, showing 6)

• CROT encodes carnitine octanoyltransferase, an enzyme known from rat studies to act on products of peroxisomal fatty acid oxidation. Medium-chain acylcarnitines are thought to be transported from peroxisomes to mitochondria. (PMID:11001805)
• Human CPT1A, CPT1B, CPT2, CROT and CRAT are known to encode active carnitine acyltransferases. Earlier pfam annotations refer to the non-existing compound CARNITATE. In 2000 this has been changed to CARNITINE. (PMID:11001805)
• Selected ‘Tpl2/Cot-YL ribozyme’ efficiently cleaves its target sequence in cis and in trans; furthermore, the ribozyme efficiently cleaves a longer target sequence of 54 nucleotides in trans, as well as the full-length mRNA. (PMID:19054068)
• These results suggest that CROT activity, by controlling the peroxisomal amount of medium chain acyls, may control the peroxisomal oxidative pathway. (PMID:21619872)
• CROT (Carnitine O-Octanoyltransferase) Is a Novel Contributing Factor in Vascular Calcification via Promoting Fatty Acid Metabolism and Mitochondrial Dysfunction. (PMID:33356393)
• Carnitine octanoyltransferase is important for the assimilation of exogenous acetyl-L-carnitine into acetyl-CoA in mammalian cells. (PMID:36587768)

Cross-species orthologs

6 orthologs

Organism	Symbol	Gene ID
danio_rerio	crot	ENSDARG00000040352
mus_musculus	Crot	ENSMUSG00000003623
rattus_norvegicus	Crot	ENSRNOG00000006779
drosophila_melanogaster	CROT	FBGN0039543
caenorhabditis_elegans		WBGENE00009622
caenorhabditis_elegans		WBGENE00011850

Paralogs (6): CHAT (ENSG00000070748), CRAT (ENSG00000095321), CPT1A (ENSG00000110090), CPT2 (ENSG00000157184), CPT1C (ENSG00000169169), CPT1B (ENSG00000205560)

Protein

Protein identifiers

Peroxisomal carnitine O-octanoyltransferase — Q9UKG9 (reviewed: Q9UKG9)

All UniProt accessions (2): Q9UKG9, C9J3D7

UniProt curated annotations — full annotation on UniProt →

Function. Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. Converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl-CoA, to its corresponding carnitine ester.

Subunit / interactions. Monomer.

Subcellular location. Peroxisome.

Pathway. Lipid metabolism; fatty acid beta-oxidation.

Similarity. Belongs to the carnitine/choline acetyltransferase family.

Isoforms (3)

UniProt ID	Names	Canonical?
Q9UKG9-1	1	yes
Q9UKG9-2	2
Q9UKG9-3	3

RefSeq proteins (3): NP_001137407, NP_001230674, NP_066974* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000542	Carn_acyl_trans	Family
IPR023213	CAT-like_dom_sf	Homologous_superfamily
IPR039551	Cho/carn_acyl_trans_1_2	Domain
IPR042231	Cho/carn_acyl_trans_2	Homologous_superfamily
IPR042572	Carn_acyl_trans_N	Homologous_superfamily

Pfam: PF00755

Enzyme classification (BRENDA):

• EC 2.3.1.137 — carnitine O-octanoyltransferase (BRENDA: 12 organisms, 103 substrates, 23 inhibitors, 63 Km, 1 kcat entries)

Substrate kinetics (BRENDA)

19 substrates with measured Km, best-characterized 15. Km ranges are aggregated across organisms/conditions.

Substrate	Km (mM)	Measurements
DECANOYL-COA	0.0002–0.794	21
L-CARNITINE	0.0948–160	16
PALMITOYL-COA	0.0005–0.6	5
ACETYL-COA	0.077–16	3
ACYL-COA	0.002–0.034	3
OCTANOYL-COA	0.015–0.024	2
ACETYL-L-CARNITINE	0.783	1
BUTYRYL-COA	0.058	1
CHOLINE	143	1
COA	0.0074	1
COASH	0.11	1
DECANOYL-L-CARNITINE	8.4	1
HEXADECANOYL-L-CARNITINE	0.036	1
HEXANOYL-COA	0.055	1
L-ACYLCARNITINE	0.0074	1

Catalyzed reactions (Rhea), 2 shown:

• octanoyl-CoA + (R)-carnitine = O-octanoyl-(R)-carnitine + CoA (RHEA:17177)
• 4,8-dimethylnonanoyl-CoA + (R)-carnitine = O-4,8-dimethylnonanoyl-(R)-carnitine + CoA (RHEA:44860)

UniProt features (20 total): modified residue 5, binding site 5, splice variant 3, sequence variant 2, sequence conflict 2, chain 1, short sequence motif 1, active site 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9UKG9-F1	97.22	0.98

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Catalytic / active sites (1): 327 (proton acceptor)

Ligand- & substrate-binding residues (5): 406; 410–417; 439; 441; 452

Post-translational modifications (5): 57, 406, 406, 1, 40

Function

Pathways and Gene Ontology

Reactome pathways

2 pathways

ID	Pathway
R-HSA-389887	Beta-oxidation of pristanoyl-CoA
R-HSA-9033241	Peroxisomal protein import

MSigDB gene sets: 234 (showing top): GSE45365_HEALTHY_VS_MCMV_INFECTION_CD8_TCELL_IFNAR_KO_UP, GSE45365_NK_CELL_VS_CD8A_DC_MCMV_INFECTION_DN, GOBP_LIPID_MODIFICATION, GOBP_FATTY_ACID_CATABOLIC_PROCESS, LEE_NEURAL_CREST_STEM_CELL_DN, GOBP_COENZYME_A_METABOLIC_PROCESS, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_UP, GRAESSMANN_RESPONSE_TO_MC_AND_DOXORUBICIN_UP, GOBP_ORGANOPHOSPHATE_METABOLIC_PROCESS, GOBP_MONOCARBOXYLIC_ACID_METABOLIC_PROCESS, GOBP_ORGANIC_ACID_TRANSPORT, GOBP_NUCLEOBASE_CONTAINING_SMALL_MOLECULE_METABOLIC_PROCESS, GOBP_GENERATION_OF_PRECURSOR_METABOLITES_AND_ENERGY, GOBP_FATTY_ACID_BETA_OXIDATION_USING_ACYL_COA_OXIDASE, NF1_Q6_01

GO Biological Process (9): generation of precursor metabolites and energy (GO:0006091), fatty acid metabolic process (GO:0006631), fatty acid beta-oxidation (GO:0006635), carnitine metabolic process (GO:0009437), fatty acid transport (GO:0015908), coenzyme A metabolic process (GO:0015936), fatty acid beta-oxidation using acyl-CoA oxidase (GO:0033540), medium-chain fatty acid metabolic process (GO:0051791), lipid metabolic process (GO:0006629)

GO Molecular Function (4): carnitine O-octanoyltransferase activity (GO:0008458), protein binding (GO:0005515), transferase activity (GO:0016740), acyltransferase activity (GO:0016746)

GO Cellular Component (3): peroxisome (GO:0005777), peroxisomal matrix (GO:0005782), cytosol (GO:0005829)

Reactome top-level categories

Rollup of top-2 pathways:

Category	Pathways
Peroxisomal lipid metabolism	1
Protein localization	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
metabolic process	1
lipid metabolic process	1
monocarboxylic acid metabolic process	1
fatty acid catabolic process	1
fatty acid ligase activity	1
fatty acid oxidation	1
amino-acid betaine metabolic process	1
lipid transport	1
monocarboxylic acid transport	1
nucleoside phosphate metabolic process	1
sulfur compound metabolic process	1
purine-containing compound metabolic process	1
fatty acid beta-oxidation	1
fatty acid metabolic process	1
primary metabolic process	1
carnitine O-acyltransferase activity	1
binding	1
catalytic activity	1
transferase activity	1
microbody	1
peroxisome	1
microbody lumen	1
cytoplasm	1
cellular anatomical structure	1

Protein interactions and networks

STRING

917 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
CROT	HADHB	P55084	777
CROT	HADH	Q16836	706
CROT	SREBF2	Q12772	622
CROT	ABCA1	O95477	614
CROT	ECHS1	P30084	593
CROT	NPC1	O15118	570
CROT	SREBF1	P36956	567
CROT	ABCG1	P45844	554
CROT	ACAA1	P09110	542
CROT	RUNDC3B	Q96NL0	538
CROT	PRKAA1	Q13131	529
CROT	ATP8B1	O43520	497
CROT	ACOX3	O15254	487
CROT	ECH1	Q13011	483
CROT	SLC25A20	O43772	483

IntAct

9 interactions, top by confidence:

A	B	Type	Score
ALB	CNOT1	psi-mi:“MI:0914”(association)	0.350
CROT	FBP1	psi-mi:“MI:0914”(association)	0.350
CD36	TTMP	psi-mi:“MI:0914”(association)	0.350
GAS2L2	ANKRD17	psi-mi:“MI:0914”(association)	0.350
CROT	KLHDC4	psi-mi:“MI:0914”(association)	0.350
FECH	POTEF	psi-mi:“MI:0914”(association)	0.350
CAT	VWA8	psi-mi:“MI:2364”(proximity)	0.270

BioGRID (25): VWA9 (Affinity Capture-MS), EIF2AK4 (Affinity Capture-MS), KLHDC4 (Affinity Capture-MS), USP25 (Affinity Capture-MS), CROT (Affinity Capture-MS), KLHDC4 (Affinity Capture-MS), USP25 (Affinity Capture-MS), FBP1 (Affinity Capture-MS), EIF2AK4 (Affinity Capture-MS), GATAD2B (Affinity Capture-MS), CROT (Proximity Label-MS), CROT (Proximity Label-MS), CROT (Affinity Capture-MS), CROT (Affinity Capture-MS), USP25 (Affinity Capture-MS)

ESM2 similar proteins: A2RTX5, A2Z8S0, B2ZGJ1, F1LN46, O19094, P07668, P11035, P11466, P13222, P17569, P18886, P23786, P28329, P32198, P32738, P32756, P32796, P36859, P43155, P47934, P49696, P50416, P52825, P52826, P56523, P80235, P97742, Q03059, Q0V9S0, Q2KJB7, Q58DK1, Q5U3U3, Q60HG9, Q63704, Q68Y62, Q6P4X5, Q704S8, Q75G68, Q7ZXE1, Q80VY9

Diamond homologs: B2ZGJ1, O19094, P11466, P13222, P18886, P32198, P32738, P43155, P47934, P52825, P52826, Q704S8, Q9DC50, Q9UKG9, P28329, P50416, P97742, Q03059, Q8HY46, Q92523, P23786, P80235, Q2KJB7, Q5U3U3, Q60HG9, Q6P4X5, Q7ZXE1, F1LN46, P07668, P32756, P32796, Q58DK1, Q63704, Q68Y62, Q90YJ9, Q924X2, Q8BGD5, Q8TCG5, Q00614

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

39 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	30
Likely benign	1
Benign	1

Top pathogenic / likely-pathogenic (0)

SpliceAI

2437 predictions. Top by Δscore:

Variant	Effect	Δscore
7:87346338:A:AG	acceptor_gain	1.0000
7:87346339:G:GG	acceptor_gain	1.0000
7:87346427:ACTGG:A	donor_loss	1.0000
7:87346429:TGGTA:T	donor_loss	1.0000
7:87346430:GGT:G	donor_loss	1.0000
7:87346431:G:GA	donor_loss	1.0000
7:87346432:T:G	donor_loss	1.0000
7:87349043:TTTA:T	acceptor_loss	1.0000
7:87349044:TTAGG:T	acceptor_loss	1.0000
7:87349045:TAGG:T	acceptor_loss	1.0000
7:87349046:A:AG	acceptor_gain	1.0000
7:87349046:AG:A	acceptor_gain	1.0000
7:87349047:G:GC	acceptor_loss	1.0000
7:87349047:G:GG	acceptor_gain	1.0000
7:87349047:GG:G	acceptor_gain	1.0000
7:87349180:TCAGG:T	donor_loss	1.0000
7:87349184:G:GA	donor_loss	1.0000
7:87349184:G:GG	donor_gain	1.0000
7:87349185:T:G	donor_loss	1.0000
7:87359204:A:AG	acceptor_gain	1.0000
7:87359205:G:GG	acceptor_gain	1.0000
7:87359205:GT:G	acceptor_gain	1.0000
7:87359317:G:GT	donor_gain	1.0000
7:87359318:A:T	donor_gain	1.0000
7:87359323:GAAAT:G	donor_gain	1.0000
7:87359327:T:G	donor_gain	1.0000
7:87359327:T:TG	donor_gain	1.0000
7:87361501:G:GT	donor_gain	1.0000
7:87361502:A:T	donor_gain	1.0000
7:87361523:G:GT	donor_gain	1.0000

AlphaMissense

4027 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
7:87377388:T:A	W306R	0.997
7:87377388:T:C	W306R	0.997
7:87391638:C:A	R451S	0.997
7:87391639:G:C	R451P	0.997
7:87392979:A:C	S544R	0.997
7:87392981:T:A	S544R	0.997
7:87392981:T:G	S544R	0.997
7:87375832:G:C	R252P	0.996
7:87391680:T:A	W465R	0.996
7:87391680:T:C	W465R	0.996
7:87359328:T:A	W80R	0.995
7:87359328:T:C	W80R	0.995
7:87375637:T:C	L221P	0.995
7:87375717:T:A	W248R	0.995
7:87375717:T:C	W248R	0.995
7:87375891:A:C	S272R	0.995
7:87375893:T:A	S272R	0.995
7:87375893:T:G	S272R	0.995
7:87361402:T:A	W85R	0.994
7:87361402:T:C	W85R	0.994
7:87361825:G:C	D174H	0.994
7:87377394:G:C	D308H	0.994
7:87381923:A:T	D331V	0.994
7:87391636:G:A	G450D	0.994
7:87369424:G:C	R199P	0.993
7:87377390:G:C	W306C	0.993
7:87377390:G:T	W306C	0.993
7:87391609:C:T	T441I	0.993
7:87392980:G:T	S544I	0.993
7:87381912:T:A	H327Q	0.992

dbSNP variants (sampled 300 via entrez): RS1000088915 (7:87389713 T>A,C), RS1000158745 (7:87358338 C>T), RS1000198256 (7:87368314 T>A), RS1000198773 (7:87377287 A>G), RS1000468452 (7:87346069 G>A), RS1000541873 (7:87369995 A>G), RS1000651115 (7:87350477 G>A), RS1000654141 (7:87376930 G>A), RS1000719203 (7:87351905 C>T), RS1000792315 (7:87375226 A>G), RS1000804986 (7:87345216 G>A,C), RS1000849009 (7:87389448 AG>A), RS1000893678 (7:87370236 G>A), RS1000927458 (7:87349988 T>C), RS1000934366 (7:87396909 T>C)

Disease associations

OMIM: gene MIM:606090 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

2 associations (top):

Study	Trait	p-value
GCST004748_126	Lung cancer	1.000000e-06
GCST006585_1202	Blood protein levels	2.000000e-13

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL2206 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

58 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Valproic Acid	affects expression, decreases methylation, increases expression	5
Cyclosporine	decreases expression	4
trichostatin A	affects cotreatment, decreases expression, increases expression	3
bisphenol A	increases methylation, decreases expression	2
sodium arsenite	decreases expression, affects cotreatment, increases abundance, increases expression	2
Air Pollutants	decreases expression, increases abundance	2
Arsenic	increases methylation, affects cotreatment, increases abundance, increases expression	2
Phenylmercuric Acetate	affects cotreatment, increases expression	2
Aflatoxin B1	decreases methylation, increases expression	2
2,4,6-tribromophenol	increases expression	1
methylmercuric chloride	decreases expression	1
triphenyl phosphate	affects expression	1
glycidyl methacrylate	decreases expression	1
decabromobiphenyl ether	increases expression	1
tris(2-butoxyethyl) phosphate	affects expression	1
perfluorooctanoic acid	increases expression	1
manganese chloride	affects cotreatment, increases abundance, increases expression	1
potassium chromate(VI)	decreases expression, affects cotreatment	1
S-(1,2-dichlorovinyl)cysteine	affects response to substance, increases expression	1
epigallocatechin gallate	affects cotreatment, decreases expression	1
di-n-butylphosphoric acid	affects expression	1
CGP 52608	affects binding, increases reaction	1
perfluoro-n-nonanoic acid	decreases expression	1
entinostat	decreases expression, affects cotreatment	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, decreases expression, increases expression	1
abrine	decreases expression	1
2,2’,4,4’-tetrabromodiphenyl ether	decreases expression	1
dorsomorphin	affects cotreatment, decreases expression, increases expression	1
pentabrominated diphenyl ether 100	decreases expression	1
jinfukang	affects cotreatment, increases expression	1

ChEMBL screening assays

4 unique, capped per target: 4 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5658344	Binding	Inhibition of COT (unknown origin) at 100 nM relative to control	Selective JAK3 Inhibitors with a Covalent Reversible Binding Mode Targeting a New Induced Fit Binding Pocket. — Cell Chem Biol

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.