CT45A5

Summary

CT45A5 (cancer/testis antigen family 45 member A5, HGNC:33270) is a protein-coding gene on chromosome Xq26.3, encoding Cancer/testis antigen family 45 member A5 (P0DMU8).

This gene represents one of a cluster of several similar genes located on the q arm of chromosome X. The genes in this cluster encode members of the cancer/testis (CT) family of antigens, and are distinct from other CT antigens. These antigens are thought to be novel therapeutic targets for human cancers. Alternative splicing results in multiple transcript variants.

Source: NCBI Gene 441521 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 6 total
• MANE Select transcript: NM_001007551

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:33270
Approved symbol	CT45A5
Name	cancer/testis antigen family 45 member A5
Location	Xq26.3
Locus type	gene with protein product
Status	Approved
Aliases	CT45-5, CT45.5
Ensembl gene	ENSG00000228836
Ensembl biotype	protein_coding
OMIM	300796
Entrez	441521

Gene structure

Transcript identifiers

Ensembl transcripts: 3 — 3 protein_coding

ENST00000487941, ENST00000617203, ENST00000698999

RefSeq mRNA: 2 — MANE Select: NM_001007551 NM_001007551, NM_001172288

CCDS: CCDS35406

Canonical transcript exons

ENST00000698999 — 5 exons

Exon	Start	End
ENSE00003635472	135781561	135781735
ENSE00003742622	135778918	135779011
ENSE00003749904	135779393	135779641
ENSE00003975371	135785237	135785503
ENSE00003975372	135775852	135777693

Expression profiles

Bgee: expression breadth broad, 45 present calls, max score 86.17.

Top tissues by expression

107 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	86.17	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	84.17	gold quality
right testis	UBERON:0004534	62.69	gold quality
testis	UBERON:0000473	62.48	gold quality
nucleus accumbens	UBERON:0001882	61.47	gold quality
left testis	UBERON:0004533	59.47	gold quality
hypothalamus	UBERON:0001898	57.79	gold quality
caudate nucleus	UBERON:0001873	57.16	gold quality
putamen	UBERON:0001874	53.12	gold quality
primary visual cortex	UBERON:0002436	50.41	gold quality
amygdala	UBERON:0001876	48.82	gold quality
temporal lobe	UBERON:0001871	48.54	gold quality
substantia nigra	UBERON:0002038	47.27	gold quality
Ammon’s horn	UBERON:0001954	47.08	gold quality
adenohypophysis	UBERON:0002196	47.00	gold quality
pituitary gland	UBERON:0000007	46.81	gold quality
prefrontal cortex	UBERON:0000451	46.68	gold quality
brain	UBERON:0000955	43.93	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	43.52	gold quality
stromal cell of endometrium	CL:0002255	40.87	silver quality
frontal cortex	UBERON:0001870	39.68	gold quality
cerebral cortex	UBERON:0000956	39.46	gold quality
sural nerve	UBERON:0015488	38.47	gold quality
anterior cingulate cortex	UBERON:0009835	37.57	gold quality
colonic epithelium	UBERON:0000397	37.20	gold quality
ventricular zone	UBERON:0003053	36.48	gold quality
cortical plate	UBERON:0005343	36.47	gold quality
superior frontal gyrus	UBERON:0002661	36.44	silver quality
bone marrow cell	CL:0002092	36.16	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	35.91	gold quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 2.

Experiment	Marker?	Max mean expression
E-HCAD-5	yes	332.16
E-MTAB-6308	yes	229.68
E-ANND-3	no	0.10

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): CTCF

miRNA regulators (miRDB)

27 targeting CT45A5, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-450A-1-3P	100.00	69.33	1837
HSA-MIR-4673	100.00	66.64	1490
HSA-MIR-6833-3P	100.00	70.63	3197
HSA-MIR-4768-5P	100.00	69.49	2861
HSA-MIR-4455	100.00	65.48	1587
HSA-MIR-4645-5P	99.98	65.81	1284
HSA-MIR-607	99.97	73.62	5593
HSA-MIR-3688-3P	99.97	72.02	2834
HSA-MIR-3671	99.90	73.04	3897
HSA-MIR-221-3P	99.86	71.56	1329
HSA-MIR-222-3P	99.86	71.35	1337
HSA-MIR-3133	99.81	70.92	3506
HSA-MIR-4668-5P	99.79	70.58	3782
HSA-MIR-586	99.65	70.40	2051
HSA-MIR-6716-5P	99.56	68.62	1244
HSA-MIR-4506	99.34	67.47	526
HSA-MIR-5006-5P	98.79	66.92	1246
HSA-MIR-501-5P	98.77	68.88	1328
HSA-MIR-4755-3P	98.77	65.59	1915
HSA-MIR-5572	98.55	65.84	970
HSA-MIR-4518	98.12	66.82	1030
HSA-MIR-6819-5P	97.96	66.59	1071
HSA-MIR-3151-3P	97.80	66.16	479
HSA-MIR-6737-5P	97.75	66.54	1044
HSA-MIR-1266-5P	97.71	66.92	1052
HSA-MIR-6812-5P	97.56	65.39	1059
HSA-MIR-3127-5P	97.52	65.24	786

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
mus_musculus	Ct45a	ENSMUSG00000064016
rattus_norvegicus	Ct45a9	ENSRNOG00000048059
drosophila_melanogaster	IntS6	FBGN0261383
caenorhabditis_elegans		WBGENE00000994

Paralogs (11): INTS6 (ENSG00000102786), INTS6L (ENSG00000165359), SAGE1 (ENSG00000181433), CT45A1 (ENSG00000268940), CT45A3 (ENSG00000269096), CT45A10 (ENSG00000269586), CT45A9 (ENSG00000270946), CT45A2 (ENSG00000271449), CT45A7 (ENSG00000273696), CT45A8 (ENSG00000278085), CT45A6 (ENSG00000278289)

Protein

Protein identifiers

Cancer/testis antigen family 45 member A5 — P0DMU8 (reviewed: P0DMU8)

Alternative names: Cancer/testis antigen 45-5, Cancer/testis antigen 45A5

All UniProt accessions (1): P0DMU8

UniProt curated annotations — full annotation on UniProt →

Subcellular location. Nucleus.

Tissue specificity. Testis specific. Expressed in cancer cell lines.

Similarity. Belongs to the CT45 family.

RefSeq proteins (2): NP_001007552, NP_001165759 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR029307	INT_SG_DDX_CT_C	Domain
IPR051113	Integrator_subunit6	Family

Pfam: PF15300

UniProt features (8 total): sequence conflict 4, region of interest 2, chain 1, compositionally biased region 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-P0DMU8-F1	70.29	0.23

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 11 (showing top): GSE45365_NK_CELL_VS_CD8A_DC_MCMV_INFECTION_DN, chrXq26, MIR450A_1_3P, MIR501_5P, HE_LIM_SUN_FETAL_LUNG_C0_MYOFIBROBLAST_1_CELL, HE_LIM_SUN_FETAL_LUNG_C0_MYOFIBROBLAST_2_CELL, PULVER_FOREY_PERTURB_ACCUMULATION_G1_S, PULVER_FOREY_PERTURB_ATTRITION_S, GSE21678_WT_VS_FOXO1_FOXO3_KO_TREG_UP, GSE25088_CTRL_VS_IL4_STIM_STAT6_KO_MACROPHAGE_UP, GSE41867_DAY8_VS_DAY15_LCMV_ARMSTRONG_EFFECTOR_CD8_TCELL_UP

GO Biological Process (0):

GO Molecular Function (0):

GO Cellular Component (1): nucleus (GO:0005634)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
intracellular membrane-bounded organelle	1

Protein interactions and networks

STRING

280 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
CT45A5	GAGE2A	Q6NT46	400
CT45A5	MAGEA10	P43363	396
CT45A5	GAGE12B	A1L429	390
CT45A5	NANOGNB	Q7Z5D8	372
CT45A5	ZNF525	Q8N782	368
CT45A5	CT47A11	Q5JQC4	348
CT45A5	MAGEB2	O15479	329
CT45A5	GLB1L2	Q8IW92	323
CT45A5	PNLIPRP3	Q17RR3	320
CT45A5	VCX3B	Q9H321	320
CT45A5	FAM180B	Q6P0A1	315
CT45A5	PNMA5	Q96PV4	310
CT45A5	CSAG2	Q9Y5P2	303
CT45A5	OR51B5	Q9H339	299
CT45A5	VCX2	Q9H322	297

IntAct

0 interactions, top by confidence:

ESM2 similar proteins: A0FKI7, A2YX04, A5WUN7, A5WV69, A7J1T2, C5DZR8, E9Q555, F4J6F6, O43310, O48767, P0DMS1, P0DMU7, P0DMU8, P0DMU9, P0DMV0, P0DMV1, P0DMV2, Q0V9S3, Q12912, Q14693, Q1LXZ9, Q2NKQ1, Q2PFD7, Q3TEL6, Q502L1, Q5DJT8, Q5R7V3, Q5T8D3, Q5XG73, Q621Z7, Q640U0, Q641E3, Q6IRN6, Q6Z8M8, Q700C2, Q758T8, Q80Y56, Q811D2, Q8AV28, Q8BLN6

Diamond homologs: A6NJ88, P0DMU7, P0DMU8, P0DMU9, P0DMV0, P0DMV1, P0DMV2, Q2TAF4, Q5DJT8, Q5HYN5, Q5JSJ4, Q5U4W6, Q6PCM2, Q7SYD9, Q8BND4, Q8NHU0, Q9NXZ1, Q9UL03, Q54Z23, Q9W485

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

6 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	3
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

780 predictions. Top by Δscore:

Variant	Effect	Δscore
X:135778914:T:G	donor_loss	1.0000
X:135778915:G:A	donor_loss	1.0000
X:135778915:G:GG	donor_gain	1.0000
X:135778915:G:T	donor_loss	1.0000
X:135778917:AGG:A	donor_loss	1.0000
X:135778920:GCAAG:G	donor_gain	1.0000
X:135778926:G:GT	donor_gain	1.0000
X:135779010:G:GT	acceptor_gain	1.0000
X:135779010:GA:G	acceptor_gain	1.0000
X:135779010:GAA:G	acceptor_gain	1.0000
X:135779010:GAAT:G	acceptor_gain	1.0000
X:135779010:GAATA:G	acceptor_gain	1.0000
X:135779011:A:AG	acceptor_gain	1.0000
X:135779011:AG:A	acceptor_loss	1.0000
X:135779012:CAG:C	acceptor_loss	1.0000
X:135779013:TCAG:T	acceptor_loss	1.0000
X:135779016:T:G	acceptor_gain	1.0000
X:135779017:A:AG	acceptor_gain	1.0000
X:135779018:C:G	acceptor_gain	1.0000
X:135779019:A:AG	acceptor_gain	1.0000
X:135779020:A:AG	acceptor_gain	1.0000
X:135779389:T:G	donor_loss	1.0000
X:135779390:G:GG	donor_gain	1.0000
X:135779390:GTAA:G	donor_loss	1.0000
X:135779391:AG:A	donor_loss	1.0000
X:135779391:AGTA:A	donor_loss	1.0000
X:135779392:AA:A	donor_gain	1.0000
X:135779392:AAG:A	donor_loss	1.0000
X:135779393:AAAGT:A	donor_loss	1.0000
X:135779640:GA:G	acceptor_gain	1.0000

AlphaMissense

0 scored. Top likely-pathogenic:

dbSNP variants (sampled 300 via entrez): RS112350322 (X:135777935 G>A), RS112497928 (X:135778002 C>T), RS112671790 (X:135781466 C>A), RS113232188 (X:135781566 T>G), RS1171976471 (X:135777274 C>A,T), RS1173670118 (X:135784197 G>C), RS1176378203 (X:135777342 C>G,T), RS1184645255 (X:135786660 G>GA), RS1187735203 (X:135777015 C>T), RS1194199495 (X:135777299 T>C), RS1202719745 (X:135777089 T>G), RS1207461356 (X:135777574 C>T), RS1211662164 (X:135786682 A>G), RS1212958851 (X:135776835 C>G,T), RS1214169454 (X:135777053 G>A)

Disease associations

OMIM: gene MIM:300796 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

14 total (human), top 14 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
methylmercuric chloride	increases expression	1
propionaldehyde	increases expression	1
bisphenol A	affects cotreatment, decreases methylation	1
sodium arsenite	increases expression	1
butyraldehyde	increases expression	1
pentanal	increases expression	1
Bortezomib	increases expression	1
Temozolomide	increases expression	1
Fulvestrant	affects cotreatment, decreases methylation	1
Aldehydes	increases expression	1
Cadmium	decreases expression	1
Oxygen	increases expression	1
Tretinoin	decreases expression	1
Valproic Acid	increases methylation	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.