CTAGE15

Summary

CTAGE15 (CTAGE family member 15, HGNC:37295) is a protein-coding gene on chromosome 7q35, encoding cTAGE family member 15 (A4D2H0).

Predicted to be involved in endoplasmic reticulum to Golgi vesicle-mediated transport; protein secretion; and vesicle cargo loading. Predicted to be located in membrane. Predicted to be active in endoplasmic reticulum exit site and endoplasmic reticulum membrane.

Source: NCBI Gene 441294 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 52 total
• MANE Select transcript: NM_001008747

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:37295
Approved symbol	CTAGE15
Name	CTAGE family member 15
Location	7q35
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000271079
Ensembl biotype	protein_coding
Entrez	441294

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 protein_coding

ENST00000420911

RefSeq mRNA: 1 — MANE Select: NM_001008747 NM_001008747

CCDS: CCDS64788

Canonical transcript exons

ENST00000420911 — 1 exons

Exon	Start	End
ENSE00003643189	143571801	143574387

Expression profiles

Bgee: expression breadth broad, 88 present calls, max score 78.95.

Top tissues by expression

115 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	78.95	silver quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	76.43	silver quality
left testis	UBERON:0004533	66.73	gold quality
testis	UBERON:0000473	65.67	gold quality
lower esophagus mucosa	UBERON:0035834	63.77	gold quality
duodenum	UBERON:0002114	60.95	gold quality
right testis	UBERON:0004534	59.42	gold quality
mucosa of transverse colon	UBERON:0004991	56.40	gold quality
gall bladder	UBERON:0002110	55.91	gold quality
skin of abdomen	UBERON:0001416	55.05	gold quality
islet of Langerhans	UBERON:0000006	54.34	gold quality
esophagus mucosa	UBERON:0002469	54.25	gold quality
zone of skin	UBERON:0000014	53.01	gold quality
left lobe of thyroid gland	UBERON:0001120	52.69	gold quality
endometrium	UBERON:0001295	52.67	gold quality
metanephros cortex	UBERON:0010533	52.59	gold quality
skin of leg	UBERON:0001511	52.03	gold quality
thyroid gland	UBERON:0002046	51.84	gold quality
mucosa of stomach	UBERON:0001199	51.37	gold quality
right lobe of liver	UBERON:0001114	50.59	gold quality
right uterine tube	UBERON:0001302	49.94	gold quality
pancreas	UBERON:0001264	49.55	gold quality
tonsil	UBERON:0002372	49.52	gold quality
right lobe of thyroid gland	UBERON:0001119	48.33	gold quality
right lung	UBERON:0002167	47.98	gold quality
minor salivary gland	UBERON:0001830	47.59	gold quality
saliva-secreting gland	UBERON:0001044	47.12	gold quality
placenta	UBERON:0001987	46.87	gold quality
body of pancreas	UBERON:0001150	46.62	gold quality
adult mammalian kidney	UBERON:0000082	46.61	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.45

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

12 targeting CTAGE15, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-1200	99.71	70.42	1838
HSA-MIR-4699-3P	99.71	70.15	3142
HSA-MIR-519A-3P	99.67	71.67	1868
HSA-MIR-519B-3P	99.67	71.67	1868
HSA-MIR-519C-3P	99.67	71.67	1870
HSA-MIR-1252-3P	99.55	67.71	2862
HSA-MIR-548G-3P	99.48	68.67	2159
HSA-MIR-548AV-3P	99.43	68.50	1721
HSA-MIR-302A-5P	99.39	68.21	1913
HSA-MIR-4705	99.10	69.10	1091
HSA-MIR-4324	99.04	70.14	1569
HSA-MIR-8055	97.62	66.09	1023

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
mus_musculus	Mia2	ENSMUSG00000021000
rattus_norvegicus	Mia2	ENSRNOG00000005101
drosophila_melanogaster	Tango1	FBGN0286898

Paralogs (10): OTOR (ENSG00000125879), MIA2 (ENSG00000150527), MIA3 (ENSG00000154305), SPZ1 (ENSG00000164299), CTAGE1 (ENSG00000212710), CTAGE9 (ENSG00000236761), MIA (ENSG00000261857), CTAGE6 (ENSG00000271321), CTAGE4 (ENSG00000288784), CTAGE8 (ENSG00000289604)

Protein

Protein identifiers

cTAGE family member 15 — A4D2H0 (reviewed: A4D2H0)

All UniProt accessions (1): A4D2H0

UniProt curated annotations — full annotation on UniProt →

Subcellular location. Membrane.

Similarity. Belongs to the cTAGE family.

RefSeq proteins (1): NP_001008747* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR051500	cTAGE_MIA/OTOR	Family

UniProt features (11 total): sequence conflict 3, region of interest 2, coiled-coil region 2, compositionally biased region 2, chain 1, transmembrane region 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-A4D2H0-F1	69.60	0.44

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 20 (showing top): GOBP_VESICLE_MEDIATED_TRANSPORT, GOBP_ENDOPLASMIC_RETICULUM_TO_GOLGI_VESICLE_MEDIATED_TRANSPORT, GOBP_SECRETION, chr7q35, GOCC_NUCLEAR_OUTER_MEMBRANE_ENDOPLASMIC_RETICULUM_MEMBRANE_NETWORK, GOCC_ORGANELLE_SUBCOMPARTMENT, GOBP_GOLGI_VESICLE_TRANSPORT, GOBP_VESICLE_CARGO_LOADING, GOBP_INTRACELLULAR_TRANSPORT, GOCC_ENDOPLASMIC_RETICULUM_EXIT_SITE, MIR1200, MIR4324, MIR302A_5P, GOBP_ESTABLISHMENT_OF_PROTEIN_LOCALIZATION, FOURATI_BLOOD_TWINRIX_AGE_25_83YO_RESPONDERS_VS_POOR_RESPONDERS_0DY_UP

GO Biological Process (3): endoplasmic reticulum to Golgi vesicle-mediated transport (GO:0006888), protein secretion (GO:0009306), vesicle cargo loading (GO:0035459)

GO Molecular Function (0):

GO Cellular Component (3): endoplasmic reticulum membrane (GO:0005789), endoplasmic reticulum exit site (GO:0070971), membrane (GO:0016020)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	2
cytoplasm	1
intercellular transport	1
intracellular transport	1
Golgi vesicle transport	1
protein transport	1
secretion by cell	1
establishment of protein localization to extracellular region	1
protein localization to extracellular region	1
transport	1
vesicle-mediated transport	1
organelle membrane	1
nuclear outer membrane-endoplasmic reticulum membrane network	1
endoplasmic reticulum subcompartment	1
endoplasmic reticulum	1

Protein interactions and networks

STRING

449 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
CTAGE15	OTOR	Q9NRC9	476
CTAGE15	NDST1	P52848	320
CTAGE15	TMEM131	Q92545	316
CTAGE15	HOXD3	P31249	310
CTAGE15	PREB	Q9HCU5	289
CTAGE15	HOXB3	P14651	276
CTAGE15	NUS1	Q96E22	264
CTAGE15	STX18	Q9P2W9	262
CTAGE15	KLHL12	Q53G59	253
CTAGE15	H2AC14	Q99878	247
CTAGE15	HOXB4	P17483	247
CTAGE15	TRAPPC2	P0DI81	242
CTAGE15	SEC16A	O15027	234
CTAGE15	TRAPPC6A	O75865	223
CTAGE15	CCDC68	Q9H2F9	223

IntAct

2 interactions, top by confidence:

A	B	Type	Score
MIA3	CTAGE15	psi-mi:“MI:0915”(physical association)	0.400

BioGRID (5): MIA3 (Proximity Label-MS), CTAGE15 (Affinity Capture-MS), CTAGE1 (Cross-Linking-MS (XL-MS)), MIA2 (Cross-Linking-MS (XL-MS)), CTAGE15 (Co-fractionation)

ESM2 similar proteins: A0JMQ7, A2BGP7, A4D2H0, A4FU28, B4F7A7, O75665, P0C219, P0CG41, Q05D60, Q15025, Q28BZ7, Q28C41, Q2T9X8, Q32LC2, Q499E4, Q4PJT6, Q4R7I4, Q5FWP9, Q5U3Z6, Q5U465, Q5XIA0, Q5XJA2, Q5ZJA3, Q6AXZ4, Q6NRH3, Q6P926, Q6PCG6, Q7M6Y5, Q80Z25, Q865V0, Q86UF2, Q86W54, Q86XR8, Q86YF9, Q86Z20, Q8BMD2, Q8CEE0, Q8IX94, Q8IYJ2, Q8IYX8

Diamond homologs: A4D2H0, A4FU28, P0CG41, Q86UF2, Q8IX94, Q8IX95, Q91ZV0, Q96PC5, Q96RT6, Q9I8P5, Q9I8P6, Q9JIE3, Q9NRC9, Q16674, Q61865, Q62946

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

52 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	46
Likely benign	6
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

43 predictions. Top by Δscore:

Variant	Effect	Δscore
7:143571949:G:GA	donor_gain	0.5700
7:143571953:T:A	donor_gain	0.4300
7:143571915:A:G	donor_gain	0.4200
7:143571900:A:T	donor_gain	0.3600
7:143573437:A:G	acceptor_gain	0.3500
7:143571948:T:A	donor_gain	0.3300
7:143571948:T:TA	donor_gain	0.3300
7:143573869:T:A	acceptor_gain	0.3200
7:143571946:GGTG:G	donor_gain	0.2700
7:143571947:GTGG:G	donor_gain	0.2700
7:143571901:GA:G	donor_gain	0.2600
7:143571995:T:TA	acceptor_gain	0.2600
7:143573258:A:T	donor_gain	0.2600
7:143574034:AAGGG:A	acceptor_gain	0.2600
7:143574035:AGGGA:A	acceptor_gain	0.2600
7:143573436:A:AG	acceptor_gain	0.2500
7:143573866:T:TA	acceptor_gain	0.2500
7:143573917:A:AG	acceptor_gain	0.2400
7:143573918:G:GG	acceptor_gain	0.2400
7:143571947:GTG:G	donor_gain	0.2300
7:143573447:G:C	acceptor_gain	0.2300
7:143574033:CAAGG:C	acceptor_gain	0.2300
7:143573635:GATGA:G	donor_gain	0.2200
7:143573653:A:AG	acceptor_gain	0.2200
7:143573654:G:GG	acceptor_gain	0.2200
7:143573641:GTTT:G	acceptor_loss	0.2100
7:143573649:CACCA:C	acceptor_loss	0.2100
7:143573650:ACCAG:A	acceptor_loss	0.2100
7:143573651:CCAGG:C	acceptor_loss	0.2100
7:143573652:CA:C	acceptor_loss	0.2100

AlphaMissense

5108 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
7:143572388:G:C	A191P	0.935
7:143572893:T:C	L359P	0.925
7:143572968:T:C	L384P	0.918
7:143572956:T:C	L380P	0.913
7:143573222:G:C	A469P	0.902
7:143572451:G:C	A212P	0.901
7:143571968:G:A	G51R	0.900
7:143571968:G:C	G51R	0.900
7:143572872:T:C	L352P	0.892
7:143573231:G:C	A472P	0.892
7:143572001:A:C	S62R	0.886
7:143572003:T:A	S62R	0.886
7:143572003:T:G	S62R	0.886
7:143571969:G:A	G51E	0.885
7:143573244:T:C	L476P	0.880
7:143572221:T:C	L135P	0.873
7:143572083:A:T	K89I	0.869
7:143572092:T:C	L92P	0.853
7:143572358:T:C	S181P	0.853
7:143572263:T:C	L149P	0.834
7:143573136:T:C	L440P	0.828
7:143573223:C:A	A469D	0.828
7:143572539:T:C	L241P	0.826
7:143573226:G:C	R470P	0.816
7:143572473:T:C	L219P	0.808
7:143573126:G:C	A437P	0.801
7:143572242:T:C	L142P	0.791
7:143572000:A:C	R61S	0.782
7:143572000:A:T	R61S	0.782
7:143572004:T:C	F63L	0.781

dbSNP variants (sampled 300 via entrez): RS1001885401 (7:143571337 C>T), RS1006223709 (7:143571760 C>T), RS1014971387 (7:143571785 T>TA), RS1015023955 (7:143570053 C>A), RS1030941718 (7:143572493 C>A,T), RS1034285030 (7:143571341 C>A), RS1045555016 (7:143573129 A>G), RS1046015126 (7:143571031 T>C,G), RS1054205754 (7:143570892 T>G), RS1055642471 (7:143574781 CACAG>C), RS111236742 (7:143571103 A>C,T), RS111582233 (7:143570666 A>T), RS112032278 (7:143570350 C>A), RS112716988 (7:143574315 G>T), RS1156706289 (7:143570711 T>A,C)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (1): male infertility (MONDO:0005372)

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST004749_16	Lung cancer in ever smokers	5.000000e-06

MeSH disease descriptors (1)

Descriptor	Name	Tree numbers
D007248	Infertility, Male	C12.100.500.430; C12.100.750.700; C12.200.294.430

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

5 total (human), top 5 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
tris(1,3-dichloro-2-propyl)phosphate	increases expression	1
obeticholic acid	increases expression	1
Benzo(a)pyrene	increases methylation	1
Smoke	decreases expression	1
S-Nitrosoglutathione	increases expression	1

Clinical trials (associated diseases)

125 trials via MONDO — disease-level, not drug-specific.

Trial	Phase	Status	Title
NCT02202382	PHASE4	COMPLETED	Effects of Korean Red Ginseng on Male Infertility
NCT02204826	PHASE4	COMPLETED	Effects of Korean Red Ginseng on Semen Parameters in Male Infertility Patients: a Randomized, Placebo-controlled, Double-blind Clinical Study
NCT03802864	PHASE4	COMPLETED	Post-operative Pain Control of Testicular Sperm Extraction Using Liposomal Bupivacaine
NCT06100432	PHASE4	ACTIVE_NOT_RECRUITING	Effect of Eurycoma Longifolia (DLBS5055) and Multivitamins (Vitamin C+Vitamin E+ β-carotene) for Infertile Males
NCT07523022	PHASE4	ENROLLING_BY_INVITATION	Comparison of the Effect of Gonadotropin and Clomiphene Citrate Treatment on Sperm Parameters and the Outcome of Assisted Reproductive Procedures in Subfertile Men Based on the APHRODITE Groups
NCT00975117	PHASE3	COMPLETED	Spermotrend in the Treatment of Male Infertility
NCT01407432	PHASE3	COMPLETED	Impact of Folates in the Care of the Male Infertility
NCT01895816	PHASE3	COMPLETED	Herbal Tonic Fertile Supplement(ZO2C5)
NCT02605070	PHASE3	TERMINATED	Pilot Study on the Effects of FSH Treatment on the Epigenetic Characteristics of Spermatozoa in Infertile Patients With Severe Oligozoospermia
NCT07402759	PHASE3	ACTIVE_NOT_RECRUITING	Impact of tdrd9 Gene Mutations in the Therapeutic Response to L-carnitine in Oligoasthenozoospermic Men
NCT01880086	PHASE2	COMPLETED	Clomiphene Citrate for the Treatment of Low Testosterone Associated With Chronic Opioid Pain Medication Administration
NCT02061384	PHASE2	COMPLETED	RA-2 13-cis Retinoic Acid (Isotretinoin)
NCT02421887	PHASE2	COMPLETED	Males, Antioxidants, and Infertility Trial
NCT05200663	PHASE2	UNKNOWN	Efficacy Comparison of Tamoxifen and Tamoxifen With Antioxidants on Semen Quality of Male With Idiopathic Infertility
NCT05290558	PHASE2	ACTIVE_NOT_RECRUITING	The Therapeutic Effects of Bu Shen Yi Jing Pill on Semen Quality in Sub Fertile Males: a Randomized Controlled Trial
NCT06091969	PHASE2	NOT_YET_RECRUITING	Supplementation for Male Subfertility
NCT01595308	PHASE1	COMPLETED	A Pilot Study to Evaluate the Effect of Pomegranate Juice on Semen Parameters in Healthy Male Volunteers
NCT02122211	PHASE1	COMPLETED	Choline Dehydrogenase and Sperm Function: Effects of Betaine
NCT02575924	PHASE1	UNKNOWN	Influence of Culture Media on Clinical Outcomes in Poor Responders or Severe Male Infertility
NCT01304927	PHASE2/PHASE3	COMPLETED	Vitamin D Supplementation and Male Infertility: The CBG-study a Randomized Clinical Trial
NCT02349945	PHASE2/PHASE3	COMPLETED	FSH Receptor Polymorphism p.N680S and Efficacy of FSH Therapy
NCT05222841	PHASE2/PHASE3	COMPLETED	The Effectiveness of Spermotrend Food Supplement in the Treatment of Male Infertility
NCT05616598	PHASE2/PHASE3	COMPLETED	Effect of New Oral Treatment for Hepatitis C Virus on Seminal Parameters
NCT02025270	PHASE1/PHASE2	COMPLETED	MSCs For Treatment of Azoospermic Patients
NCT04541459	EARLY_PHASE1	UNKNOWN	Validation of New Devices Against Ambient Electromagnetic Radiation
NCT05792813	EARLY_PHASE1	UNKNOWN	Efficacy and Safety of Linggui Yangyuan Paste in Patients With Male Infertility
NCT06188936	EARLY_PHASE1	COMPLETED	Home Semen Analysis Tests As a Screening Tool for Fertility Patients
NCT00012480	Not specified	COMPLETED	Effect of Environmental Exposures on the Egg Fertilizing Ability of Human Sperm
NCT00044369	Not specified	COMPLETED	Role of the Toxic Metal Cadmium in the Mechanism Producing Infertility With a Varicocele
NCT00119925	Not specified	UNKNOWN	‘SPRING’-Study: Subfertility Guidelines: Patient Related Implementation in the Netherlands Among Gynaecologists
NCT00178516	Not specified	COMPLETED	Vitamin E and Male Infertility
NCT00315029	Not specified	COMPLETED	Patient-Centered Implementation Trial for Single Embryo Transfer
NCT00341120	Not specified	COMPLETED	Genetic Causes of Male Infertility
NCT00481403	Not specified	COMPLETED	Study of Sperm Molecular Factors Implicated in Male Fertility
NCT00548977	Not specified	COMPLETED	Genetic Studies Spermatogenic Failure
NCT00596739	Not specified	COMPLETED	A Study of the Pre- and Post-operative Semen Analyses and Reproductive Hormone Levels of Men Undergoing Weight-reduction Surgery
NCT00756561	Not specified	COMPLETED	HOP-2A - Intratesticular Hormone Levels
NCT00961558	Not specified	TERMINATED	Canadian Varicocelectomy Initiative (CVI): Effects on Male Fertility and Testicular Function of Varicocelectomy
NCT01075334	Not specified	UNKNOWN	Is a Carnitine Based Food Supplement (PorimoreTM) for Infertile Men Superior to Folate and Zinc With Regard to Pregnancy Rates in Intrauterine Insemination Cycles?
NCT01178463	Not specified	UNKNOWN	Spermatogonial Stem Cells in Azoospermic Patients: a Comparison Between Obstructive and Non-obstructive Azoospermia