DCAF10

Summary

DCAF10 (DDB1 and CUL4 associated factor 10, HGNC:23686) is a protein-coding gene on chromosome 9p13.2, encoding DDB1- and CUL4-associated factor 10 (Q5QP82). May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.

Predicted to be involved in protein ubiquitination. Part of Cul4-RING E3 ubiquitin ligase complex.

Source: NCBI Gene 79269 — RefSeq curated summary.

At a glance

• GWAS associations: 3
• Clinical variants (ClinVar): 57 total
• MANE Select transcript: NM_024345

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:23686
Approved symbol	DCAF10
Name	DDB1 and CUL4 associated factor 10
Location	9p13.2
Locus type	gene with protein product
Status	Approved
Aliases	MGC10765, FLJ23201
Ensembl gene	ENSG00000122741
Ensembl biotype	protein_coding
OMIM	620295
Entrez	79269

Gene structure

Transcript identifiers

Ensembl transcripts: 9 — 6 protein_coding, 3 protein_coding_CDS_not_defined

ENST00000242323, ENST00000377724, ENST00000461549, ENST00000478453, ENST00000483167, ENST00000878417, ENST00000878418, ENST00000878419, ENST00000878420

RefSeq mRNA: 2 — MANE Select: NM_024345 NM_001286810, NM_024345

CCDS: CCDS6613, CCDS75835

Canonical transcript exons

ENST00000377724 — 7 exons

Exon	Start	End
ENSE00001418498	37819288	37819401
ENSE00001433066	37842089	37842286
ENSE00001836891	37800797	37801405
ENSE00003462136	37854780	37854982
ENSE00003514965	37861140	37867664
ENSE00003516403	37857241	37857351
ENSE00003658919	37860048	37860193

Expression profiles

Bgee: expression breadth ubiquitous, 281 present calls, max score 98.37.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 18.4996 / max 112.1127, expressed in 1809 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
96747	18.4996	1809

Top tissues by expression

292 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
sperm	CL:0000019	98.37	gold quality
male germ cell	CL:0000015	94.16	gold quality
left testis	UBERON:0004533	92.99	gold quality
adrenal tissue	UBERON:0018303	92.41	gold quality
right testis	UBERON:0004534	92.35	gold quality
stromal cell of endometrium	CL:0002255	91.73	gold quality
testis	UBERON:0000473	91.38	gold quality
mucosa of paranasal sinus	UBERON:0005030	90.57	gold quality
islet of Langerhans	UBERON:0000006	90.46	gold quality
lower esophagus mucosa	UBERON:0035834	90.01	gold quality
gastrocnemius	UBERON:0001388	88.46	gold quality
cartilage tissue	UBERON:0002418	88.27	gold quality
muscle of leg	UBERON:0001383	88.01	gold quality
right adrenal gland	UBERON:0001233	87.94	gold quality
adrenal gland	UBERON:0002369	87.52	gold quality
right adrenal gland cortex	UBERON:0035827	87.35	gold quality
adult organism	UBERON:0007023	87.28	gold quality
left adrenal gland	UBERON:0001234	87.27	gold quality
left adrenal gland cortex	UBERON:0035825	87.00	gold quality
adrenal cortex	UBERON:0001235	86.48	gold quality
rectum	UBERON:0001052	86.46	gold quality
ganglionic eminence	UBERON:0004023	86.43	gold quality
cortical plate	UBERON:0005343	86.31	gold quality
pancreas	UBERON:0001264	86.26	gold quality
hindlimb stylopod muscle	UBERON:0004252	85.95	gold quality
skeletal muscle organ	UBERON:0014892	85.69	gold quality
muscle organ	UBERON:0001630	85.68	gold quality
esophagus mucosa	UBERON:0002469	85.35	gold quality
body of pancreas	UBERON:0001150	85.24	gold quality
mammary duct	UBERON:0001765	85.13	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 2.

Experiment	Marker?	Max mean expression
E-MTAB-6819	yes	3995.96
E-ANND-3	yes	9.26

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

288 targeting DCAF10, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-518D-5P	100.00	67.51	979
HSA-MIR-518E-5P	100.00	67.66	954
HSA-MIR-518F-5P	100.00	67.51	979
HSA-MIR-519A-5P	100.00	67.66	954
HSA-MIR-519B-5P	100.00	67.66	954
HSA-MIR-519C-5P	100.00	67.66	954
HSA-MIR-520C-5P	100.00	67.51	979
HSA-MIR-522-5P	100.00	67.66	954
HSA-MIR-523-5P	100.00	67.66	954
HSA-MIR-526A-5P	100.00	67.51	979
HSA-MIR-5692A	100.00	74.40	6850
HSA-MIR-6758-5P	100.00	66.21	1470
HSA-MIR-6867-5P	100.00	82.21	3464
HSA-MIR-6856-5P	100.00	65.47	1298
HSA-MIR-4668-3P	100.00	68.74	2635
HSA-MIR-126-5P	100.00	72.71	3180
HSA-MIR-4795-3P	100.00	74.62	4024
HSA-MIR-4510	100.00	66.60	2050
HSA-MIR-6127	100.00	66.76	2188
HSA-MIR-6129	100.00	66.46	2080
HSA-MIR-6130	100.00	66.69	2012
HSA-MIR-6133	100.00	66.48	2064
HSA-MIR-190A-3P	100.00	80.35	5520
HSA-MIR-3689D	100.00	66.14	1181
HSA-MIR-6851-5P	100.00	65.63	1294
HSA-MIR-4283	100.00	66.42	2097
HSA-MIR-1252-5P	100.00	69.80	2774
HSA-MIR-4476	100.00	68.18	2030
HSA-MIR-6876-5P	100.00	67.68	2126
HSA-MIR-4673	100.00	66.64	1490

Literature-anchored findings (GeneRIF, showing 1)

• Adenovirus E1A binding to DCAF10 targets proteasomal degradation of RUVBL1/2 AAA+ ATPases required for quaternary assembly of multiprotein machines, innate immunity, and responses to metabolic stress. (PMID:37962355)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	wdr32	ENSDARG00000029600
mus_musculus	Dcaf10	ENSMUSG00000035572
rattus_norvegicus	Dcaf10	ENSRNOG00000011655
drosophila_melanogaster	CG1523	FBGN0039601

Protein

Protein identifiers

DDB1- and CUL4-associated factor 10 — Q5QP82 (reviewed: Q5QP82)

Alternative names: WD repeat-containing protein 32

All UniProt accessions (2): A0A0A0MQT4, Q5QP82

UniProt curated annotations — full annotation on UniProt →

Function. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.

Subunit / interactions. Interacts with DDB1.

Pathway. Protein modification; protein ubiquitination.

Similarity. Belongs to the WD repeat DCAF10 family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q5QP82-1	1	yes
Q5QP82-2	2

RefSeq proteins (2): NP_001273739, NP_077321* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001680	WD40_rpt	Repeat
IPR015943	WD40/YVTN_repeat-like_dom_sf	Homologous_superfamily
IPR036322	WD40_repeat_dom_sf	Homologous_superfamily
IPR039085	DCA10	Family

Pfam: PF00400

UniProt features (24 total): repeat 7, modified residue 6, compositionally biased region 4, sequence conflict 3, region of interest 2, chain 1, splice variant 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q5QP82-F1	70.93	0.44

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (6): 53, 63, 89, 92, 134, 349

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-8951664	Neddylation

MSigDB gene sets: 156 (showing top): GSE45365_CD8A_DC_VS_CD11B_DC_IFNAR_KO_MCMV_INFECTION_UP, GSE18804_SPLEEN_MACROPHAGE_VS_BRAIN_TUMORAL_MACROPHAGE_DN, CGGAARNGGCNG_UNKNOWN, IVANOVA_HEMATOPOIESIS_MATURE_CELL, SMID_BREAST_CANCER_LUMINAL_B_UP, chr9p13, MODULE_480, GOBP_POST_TRANSLATIONAL_PROTEIN_MODIFICATION, NAKAMURA_TUMOR_ZONE_PERIPHERAL_VS_CENTRAL_DN, MODULE_427, GOCC_TRANSFERASE_COMPLEX, MODULE_192, GOCC_CUL4_RING_E3_UBIQUITIN_LIGASE_COMPLEX, GOCC_CULLIN_RING_UBIQUITIN_LIGASE_COMPLEX, SCGGAAGY_ELK1_02

GO Biological Process (1): protein ubiquitination (GO:0016567)

GO Molecular Function (1): protein binding (GO:0005515)

GO Cellular Component (2): nucleoplasm (GO:0005654), Cul4-RING E3 ubiquitin ligase complex (GO:0080008)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
Post-translational protein modification	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
protein modification by small protein conjugation	1
binding	1
nuclear lumen	1
cellular anatomical structure	1
cullin-RING ubiquitin ligase complex	1

Protein interactions and networks

STRING

1152 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
DCAF10	C17orf75	Q9HAS0	573
DCAF10	C1GALT1C1L	P0DN25	555
DCAF10	DCAF5	Q96JK2	533
DCAF10	CYP20A1	Q6UW02	514
DCAF10	DCAF4	Q8WV16	509
DCAF10	NUDT12	Q9BQG2	502
DCAF10	KLHDC10	Q6PID8	491
DCAF10	DYNC2H1	Q8NCM8	479
DCAF10	CIAO1	O76071	476
DCAF10	FKBP14	Q9NWM8	474
DCAF10	CACNG8	Q8WXS5	474
DCAF10	SYNJ2BP	P57105	461
DCAF10	FRMPD1	Q5SYB0	458
DCAF10	RAB3IP	Q96QF0	437
DCAF10	SAYSD1	Q9NPB0	431

IntAct

119 interactions, top by confidence:

A	B	Type	Score
CUL4B	COPS2	psi-mi:“MI:0914”(association)	0.790
COPS6	RHOBTB1	psi-mi:“MI:0914”(association)	0.730
PPP2R2C	PPP2R1A	psi-mi:“MI:0914”(association)	0.730
CUL4A	COPS2	psi-mi:“MI:0914”(association)	0.640
SLX4	ERCC1	psi-mi:“MI:0914”(association)	0.640
EIF3I	EIF3CL	psi-mi:“MI:0914”(association)	0.640
PPP2R2C	TCP1	psi-mi:“MI:0914”(association)	0.640
CCT3	TXNDC9	psi-mi:“MI:0914”(association)	0.640
EIF3I	EIF3F	psi-mi:“MI:0914”(association)	0.640
NDUFAF4	NDUFS8	psi-mi:“MI:0914”(association)	0.640
SPG11	AP5Z1	psi-mi:“MI:0914”(association)	0.620
DCAF10	LHX4	psi-mi:“MI:0915”(physical association)	0.560
DCAF10	DNAJA2	psi-mi:“MI:0915”(physical association)	0.560
TCIRG1	AP3D1	psi-mi:“MI:0914”(association)	0.530
TNFRSF13B	TNFRSF10B	psi-mi:“MI:0914”(association)	0.530
SPINK4	PLXNA2	psi-mi:“MI:0914”(association)	0.530
ZMAT5	DENND4B	psi-mi:“MI:0914”(association)	0.530
TRUB2	HSPD1	psi-mi:“MI:0914”(association)	0.530
STK16	FLG	psi-mi:“MI:0914”(association)	0.530
SLAIN1	HSPA9	psi-mi:“MI:0914”(association)	0.530
GNAZ	MTHFR	psi-mi:“MI:0914”(association)	0.530
DIRAS3	MTIF2	psi-mi:“MI:0914”(association)	0.530
PPIL2	YWHAH	psi-mi:“MI:0914”(association)	0.530
FBXL19	MED19	psi-mi:“MI:0914”(association)	0.530
HSPB8	VWA8	psi-mi:“MI:0914”(association)	0.530
YJU2B	RCCD1	psi-mi:“MI:0914”(association)	0.530
STK16	UNC119B	psi-mi:“MI:0914”(association)	0.530

BioGRID (268): DCAF10 (Affinity Capture-MS), DCAF10 (Affinity Capture-MS), DCAF10 (Affinity Capture-MS), DCAF10 (Affinity Capture-MS), DCAF10 (Affinity Capture-MS), DCAF10 (Affinity Capture-MS), DCAF10 (Affinity Capture-MS), DCAF10 (Affinity Capture-MS), DCAF10 (Affinity Capture-MS), DCAF10 (Biochemical Activity), DCAF10 (Affinity Capture-MS), DCAF10 (Affinity Capture-MS), DCAF10 (Affinity Capture-MS), DCAF10 (Affinity Capture-MS), DCAF10 (Affinity Capture-MS)

ESM2 similar proteins: A2AKB9, A2RRH5, A2RRU4, A2SXS5, A6QM06, O60346, O88559, P30291, P97260, Q08BB3, Q0P5I0, Q12770, Q3B7L5, Q3MHH0, Q3UHE1, Q4R3J7, Q5E9I8, Q5FW06, Q5M9G8, Q5MNU5, Q5QP82, Q5R7H5, Q5T6F0, Q5VW00, Q5ZJL7, Q63ZP7, Q69Z89, Q6AX81, Q6GQT6, Q6NS60, Q6NWH1, Q6P809, Q6ZWB6, Q8AVS9, Q8BGW4, Q8BGZ3, Q8CHE4, Q8NHY2, Q8QZS3, Q8TEB1

Diamond homologs: A2AKB9, B4JSW8, B4M4W4, C7Z6H2, O14021, Q4ICM0, Q4PSE4, Q5FW06, Q5QP82, Q5RHI5, Q6NWH1, Q8AVS9, Q9VAT2, A7RHG8, B2AEZ5, B2B766, B4JPT9, B9WD30, C4YPI7, C5MJE8, O22466, O22467, O22468, O22469, O93377, O94244, P0CS36, P0CS37, P39984, P90916, Q03897, Q09028, Q10G81, Q16576, Q24572, Q2HBX6, Q2UA71, Q3MHL3, Q3SWX8, Q4I7L0

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 154 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
DNA Damage Recognition in GG-NER	8	23.6×	8e-07
Formation of TC-NER Pre-Incision Complex	6	13.1×	2e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

57 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	49
Likely benign	3
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

2159 predictions. Top by Δscore:

Variant	Effect	Δscore
9:37819287:GGTCA:G	acceptor_gain	1.0000
9:37860191:G:GT	donor_gain	1.0000
9:37861138:A:AG	acceptor_gain	1.0000
9:37861139:G:GG	acceptor_gain	1.0000
9:37861139:GT:G	acceptor_gain	1.0000
9:37861139:GTGT:G	acceptor_gain	1.0000
9:37861139:GTGTA:G	acceptor_gain	1.0000
9:37801401:GACGG:G	donor_gain	0.9900
9:37801404:GG:G	donor_gain	0.9900
9:37801404:GGGTA:G	donor_loss	0.9900
9:37801405:GG:G	donor_gain	0.9900
9:37801406:GTAA:G	donor_loss	0.9900
9:37801407:T:TC	donor_loss	0.9900
9:37831490:AGTT:A	donor_gain	0.9900
9:37842171:G:GG	donor_gain	0.9900
9:37857239:A:G	acceptor_gain	0.9900
9:37860047:GGA:G	acceptor_gain	0.9900
9:37860299:GGCA:G	donor_gain	0.9900
9:37861136:CTAGT:C	acceptor_loss	0.9900
9:37861138:AGT:A	acceptor_gain	0.9900
9:37861139:G:GT	acceptor_loss	0.9900
9:37861139:GTG:G	acceptor_gain	0.9900
9:37801406:G:GG	donor_gain	0.9800
9:37819397:ATCAG:A	donor_loss	0.9800
9:37819398:TCAG:T	donor_loss	0.9800
9:37819399:CAG:C	donor_loss	0.9800
9:37819400:AG:A	donor_loss	0.9800
9:37819401:GGTT:G	donor_loss	0.9800
9:37819402:G:GC	donor_loss	0.9800
9:37819403:T:A	donor_loss	0.9800

AlphaMissense

3572 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
9:37801380:T:C	F172L	1.000
9:37801382:C:A	F172L	1.000
9:37801382:C:G	F172L	1.000
9:37801392:T:G	Y176D	1.000
9:37801404:G:A	G180R	1.000
9:37801404:G:C	G180R	1.000
9:37801404:G:T	G180W	1.000
9:37801405:G:A	G180E	1.000
9:37801405:G:T	G180V	1.000
9:37819296:T:C	L183P	1.000
9:37819326:T:C	L193P	1.000
9:37819329:T:C	L194P	1.000
9:37819393:T:A	N215K	1.000
9:37819393:T:G	N215K	1.000
9:37842090:T:C	F219L	1.000
9:37842091:T:C	F219S	1.000
9:37842092:T:A	F219L	1.000
9:37842092:T:G	F219L	1.000
9:37842109:T:C	F225S	1.000
9:37842112:C:A	A226D	1.000
9:37842119:C:G	C228W	1.000
9:37842120:T:C	S229P	1.000
9:37842121:C:A	S229Y	1.000
9:37842121:C:T	S229F	1.000
9:37842123:G:C	D230H	1.000
9:37842124:A:C	D230A	1.000
9:37842124:A:G	D230G	1.000
9:37842124:A:T	D230V	1.000
9:37842126:G:C	D231H	1.000
9:37842126:G:T	D231Y	1.000

dbSNP variants (sampled 300 via entrez): RS1000019794 (9:37831873 G>A), RS1000111989 (9:37865634 G>C), RS1000160190 (9:37810982 G>A,C), RS1000172251 (9:37840220 A>G), RS1000179746 (9:37818642 C>T), RS1000204844 (9:37840619 G>A), RS1000221404 (9:37824305 C>T), RS1000313758 (9:37802759 A>G), RS1000452317 (9:37848421 A>G), RS1000504888 (9:37863422 A>C), RS1000526253 (9:37822156 A>C,G), RS1000609190 (9:37855070 A>G), RS1000695952 (9:37836954 A>G), RS1000697103 (9:37819741 T>C,G), RS1000758227 (9:37811824 G>A)

Disease associations

OMIM: gene MIM:620295 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

3 associations (top):

Study	Trait	p-value
GCST003772_13	Loneliness (linear analysis)	2.000000e-06
GCST90002385_381	High light scatter reticulocyte count	3.000000e-09
GCST90002405_457	Reticulocyte count	2.000000e-09

EFO canonical traits (2, from GWAS)

EFO ID	Trait name
EFO:0007865	loneliness measurement
EFO:0007986	reticulocyte count

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

25 total (human), top 25 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Valproic Acid	increases expression	4
Air Pollutants	increases expression, decreases expression, increases abundance	2
Particulate Matter	decreases expression, increases abundance, increases expression	2
GSK-J4	increases expression	1
FR900359	affects phosphorylation	1
methylmercuric chloride	decreases expression	1
triphenyl phosphate	affects expression	1
trichostatin A	increases expression	1
tris(1,3-dichloro-2-propyl)phosphate	increases expression	1
butyraldehyde	decreases expression	1
coumarin	increases phosphorylation	1
ICG 001	increases expression	1
abrine	increases expression	1
jinfukang	decreases expression	1
Sunitinib	increases expression	1
Leflunomide	increases expression	1
Atrazine	increases expression	1
Caffeine	decreases phosphorylation	1
Carbamazepine	affects expression	1
Doxorubicin	decreases expression	1
Ivermectin	decreases expression	1
Tobacco Smoke Pollution	increases expression	1
Cyclosporine	increases expression	1
Antirheumatic Agents	increases expression	1
Cadmium Chloride	decreases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.