DCAF7

Summary

DCAF7 (DDB1 and CUL4 associated factor 7, HGNC:30915) is a protein-coding gene on chromosome 17q23.3, encoding DDB1- and CUL4-associated factor 7 (P61962). Involved in craniofacial development. It is a selective cancer dependency (DepMap: 66.2% of cell lines).

This gene encodes a protein with multiple WD40 repeats which facilitate protein-protein interactions and thereby enable the assembly of multiprotein complexes. This protein has been shown to function as a scaffold protein for protein complexes involved in kinase signaling. This highly conserved gene is present in eukaryotic plants, fungi, and animals. The ortholog of this gene was first identified in plants as a key regulator of anthocyanin biosynthesis and flower pigmentation. Alternative splicing results in multiple transcript variants.

Source: NCBI Gene 10238 — RefSeq curated summary.

At a glance

• GWAS associations: 2
• Clinical variants (ClinVar): 21 total
• Druggable target: yes
• Cancer dependency (DepMap): dependent in 66.2% of screened cell lines
• MANE Select transcript: NM_005828

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:30915
Approved symbol	DCAF7
Name	DDB1 and CUL4 associated factor 7
Location	17q23.3
Locus type	gene with protein product
Status	Approved
Aliases	HAN11, SWAN-1
Ensembl gene	ENSG00000136485
Ensembl biotype	protein_coding
OMIM	605973
Entrez	10238

Gene structure

Transcript identifiers

Ensembl transcripts: 19 — 9 protein_coding, 6 nonsense_mediated_decay, 4 retained_intron

ENST00000415273, ENST00000431926, ENST00000580091, ENST00000582274, ENST00000614556, ENST00000615512, ENST00000686337, ENST00000686787, ENST00000688437, ENST00000688721, ENST00000688888, ENST00000688972, ENST00000690417, ENST00000690481, ENST00000691063, ENST00000691458, ENST00000692877, ENST00000693226, ENST00000693510

RefSeq mRNA: 1 — MANE Select: NM_005828 NM_005828

CCDS: CCDS74127

Canonical transcript exons

ENST00000614556 — 7 exons

Exon	Start	End
ENSE00001758950	63589000	63594266
ENSE00001781109	63579825	63579943
ENSE00003463995	63585211	63585328
ENSE00003555369	63579337	63579448
ENSE00003602780	63578470	63578628
ENSE00003729760	63550477	63550815
ENSE00003743456	63583502	63583711

Expression profiles

Bgee: expression breadth ubiquitous, 285 present calls, max score 97.27.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 59.5984 / max 309.8919, expressed in 1828 samples.

FANTOM5 promoters (4 alternative TSS)

Promoter ID	TPM avg	Samples expressed
162200	40.2534	1825
162202	16.1010	1780
162201	2.8543	1298
162203	0.3897	255

Top tissues by expression

294 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
ventricular zone	UBERON:0003053	97.27	gold quality
ganglionic eminence	UBERON:0004023	96.95	gold quality
oocyte	CL:0000023	96.78	gold quality
cortical plate	UBERON:0005343	96.39	gold quality
secondary oocyte	CL:0000655	95.62	gold quality
renal medulla	UBERON:0000362	95.19	gold quality
cardia of stomach	UBERON:0001162	94.38	gold quality
trabecular bone tissue	UBERON:0002483	94.31	gold quality
lower lobe of lung	UBERON:0008949	93.94	gold quality
embryo	UBERON:0000922	93.82	gold quality
ventral tegmental area	UBERON:0002691	93.82	gold quality
inferior vagus X ganglion	UBERON:0005363	93.80	gold quality
subthalamic nucleus	UBERON:0001906	93.76	gold quality
mucosa of sigmoid colon	UBERON:0004993	93.73	gold quality
pylorus	UBERON:0001166	93.66	gold quality
urethra	UBERON:0000057	93.36	gold quality
stromal cell of endometrium	CL:0002255	93.21	gold quality
superior vestibular nucleus	UBERON:0007227	93.19	gold quality
nipple	UBERON:0002030	93.16	gold quality
colonic mucosa	UBERON:0000317	93.08	gold quality
islet of Langerhans	UBERON:0000006	92.88	gold quality
trigeminal ganglion	UBERON:0001675	92.83	gold quality
pigmented layer of retina	UBERON:0001782	92.77	gold quality
parietal lobe	UBERON:0001872	92.61	gold quality
postcentral gyrus	UBERON:0002581	92.58	gold quality
caput epididymis	UBERON:0004358	92.57	gold quality
medial globus pallidus	UBERON:0002477	92.54	gold quality
globus pallidus	UBERON:0001875	92.53	gold quality
monocyte	CL:0000576	92.48	gold quality
corpus epididymis	UBERON:0004359	92.47	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-CURD-112	no	2.11
E-ANND-3	no	0.00

Regulation

Is transcription factor: yes

Downstream targets (CollecTRI)

1 targets.

Target	Regulation
GLI1	Repression

miRNA regulators (miRDB)

318 targeting DCAF7, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-LET-7A-3P	100.00	74.03	3932
HSA-LET-7B-3P	100.00	74.08	3913
HSA-LET-7F-1-3P	100.00	74.02	3928
HSA-MIR-98-3P	100.00	74.08	3907
HSA-MIR-29A-3P	100.00	73.11	1835
HSA-MIR-29B-3P	100.00	73.18	1833
HSA-MIR-29C-3P	100.00	73.15	1833
HSA-MIR-5193	100.00	67.26	1744
HSA-MIR-6876-5P	100.00	67.68	2126
HSA-MIR-8485	100.00	77.57	4731
HSA-MIR-513A-5P	100.00	69.77	2465
HSA-MIR-4476	100.00	68.18	2030
HSA-MIR-4692	100.00	67.32	2066
HSA-MIR-5011-5P	100.00	83.46	5820
HSA-MIR-5692B	100.00	71.32	2622
HSA-MIR-5692C	100.00	71.32	2622
HSA-MIR-5196-5P	100.00	67.98	2761
HSA-MIR-4747-5P	100.00	67.90	2681
HSA-MIR-432-3P	100.00	67.86	705
HSA-MIR-1277-5P	100.00	73.95	5056
HSA-MIR-188-3P	100.00	68.76	1240
HSA-MIR-3064-3P	100.00	70.09	1254
HSA-MIR-6748-5P	100.00	65.81	1057
HSA-MIR-4282	99.99	75.36	6408
HSA-MIR-34A-5P	99.99	71.21	1784
HSA-MIR-449A	99.99	71.05	1776
HSA-MIR-6077	99.99	68.04	2299
HSA-MIR-4514	99.99	67.10	1870
HSA-MIR-6870-5P	99.99	68.55	2115
HSA-MIR-520D-5P	99.98	73.34	4883

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 66.2% of screened cell lines.

Literature-anchored findings (GeneRIF, showing 9)

• that AN11 may be a physiological regulator of GLI1 transcriptional activity (PMID:16887337)
• Han11 was required to allow coupling of MEKK1 to DYRK1 and HIPK2. (PMID:20940704)
• DYRK1A binds specifically to WDR68 in cells; the binding, but not the phosphorylation event, induces the nuclear translocation of WDR68. (PMID:21777625)
• results demonstrate that the molecular chaperone TRiC/CCT is essential for correct protein folding, DYRK1A binding, and nuclear accumulation of WDR68. (PMID:25342745)
• Immunoprecipitation and pulldown experiments identified DCAF7 as an adaptor for the association of the adenovirus E1A protein with DYRK1A and HIPK2 (PMID:27307198)
• knockdown of DCAF7 reduced the degradation of DNA ligase I in response to inhibition of proliferation and replacement of ubiquitylated lysine residues reduced the in vitro ubiquitylation of DNA ligase I by Cul4-DDB1 and DCAF7. In contrast, a different E3 ubiquitin ligase regulates FEN-1 turnover. (PMID:27573245)
• DCAF7/WDR68 is required for normal levels of DYRK1A and DYRK1B (PMID:30496304)
• DCAF7 is a novel regulator of ERCC1-XPF protein level and cellular nucleotide excision repair activity. (PMID:31493872)
• MEN1 Degradation Induced by Neddylation and the CUL4B-DCAF7 Axis Promotes Pancreatic Neuroendocrine Tumor Progression. (PMID:36939378)

Cross-species orthologs

6 orthologs

Organism	Symbol	Gene ID
danio_rerio	dcaf7	ENSDARG00000054355
mus_musculus	Dcaf7	ENSMUSG00000049354
rattus_norvegicus	Dcaf7	ENSRNOG00000042245
drosophila_melanogaster	wap	FBGN0266848
caenorhabditis_elegans		WBGENE00009976
caenorhabditis_elegans		WBGENE00009977

Protein

Protein identifiers

DDB1- and CUL4-associated factor 7 — P61962 (reviewed: P61962)

Alternative names: WD repeat-containing protein 68, WD repeat-containing protein An11 homolog

All UniProt accessions (10): A0A087WWI6, A0A8I5KQ63, A0A8I5KRG1, A0A8I5KT41, A0A8I5KTU5, A0A8I5KX45, A0A8I5KXF0, A0A8I5KY66, A0A8I5QKQ1, P61962

UniProt curated annotations — full annotation on UniProt →

Function. Involved in craniofacial development. Acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway function differs between the first and second arches. Associates with DIAPH1 and controls GLI1 transcriptional activity. Could be involved in normal and disease skin development. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.

Subunit / interactions. Interacts with DYRK1A, DYRK1B and DIAPH1. Interacts with DDB1. Interacts with ZNF703. Interacts with human adenovirus 5 E1A protein.

Subcellular location. Cytoplasm. Nucleus.

Pathway. Protein modification; protein ubiquitination.

Similarity. Belongs to the WD repeat DCAF7 family.

Isoforms (2)

UniProt ID	Names	Canonical?
P61962-1	1	yes
P61962-2	2

RefSeq proteins (1): NP_005819* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001680	WD40_rpt	Repeat
IPR015943	WD40/YVTN_repeat-like_dom_sf	Homologous_superfamily
IPR019775	WD40_repeat_CS	Conserved_site
IPR036322	WD40_repeat_dom_sf	Homologous_superfamily
IPR045159	DCAF7-like	Family

Pfam: PF00400

UniProt features (9 total): repeat 7, chain 1, splice variant 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-P61962-F1	93.35	0.87

Function

Pathways and Gene Ontology

Reactome pathways

2 pathways

ID	Pathway
R-HSA-390471	Association of TriC/CCT with target proteins during biosynthesis
R-HSA-8951664	Neddylation

MSigDB gene sets: 236 (showing top): TGGTGCT_MIR29A_MIR29B_MIR29C, AGGAAGC_MIR5163P, ACTACCT_MIR196A_MIR196B, TGCTGCT_MIR15A_MIR16_MIR15B_MIR195_MIR424_MIR497, BROWNE_HCMV_INFECTION_16HR_UP, MODULE_16, ACTGCAG_MIR173P, AATGGAG_MIR136, ATGTTAA_MIR302C, PATIL_LIVER_CANCER, PUJANA_CHEK2_PCC_NETWORK, BLALOCK_ALZHEIMERS_DISEASE_UP, MODULE_213, TGACATY_UNKNOWN, GOBP_POST_TRANSLATIONAL_PROTEIN_MODIFICATION

GO Biological Process (1): protein ubiquitination (GO:0016567)

GO Molecular Function (2): protein-macromolecule adaptor activity (GO:0030674), protein binding (GO:0005515)

GO Cellular Component (8): nucleus (GO:0005634), nucleoplasm (GO:0005654), cytoplasm (GO:0005737), cytosol (GO:0005829), nuclear matrix (GO:0016363), nuclear body (GO:0016604), protein-containing complex (GO:0032991), Cul4-RING E3 ubiquitin ligase complex (GO:0080008)

Reactome top-level categories

Rollup of top-2 pathways:

Category	Pathways
Chaperonin-mediated protein folding	1
Post-translational protein modification	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	4
nuclear lumen	2
protein modification by small protein conjugation	1
protein binding	1
molecular adaptor activity	1
binding	1
intracellular membrane-bounded organelle	1
intracellular anatomical structure	1
cytoplasm	1
nucleoplasm	1
intracellular membraneless organelle	1
cellular_component	1
cullin-RING ubiquitin ligase complex	1

Protein interactions and networks

STRING

1528 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
DCAF7	PAX6	P26367	993
DCAF7	DYRK1A	Q13627	938
DCAF7	DDB1	Q16531	888
DCAF7	DYRK1B	Q9Y463	863
DCAF7	NCOR2	Q9Y618	787
DCAF7	CUL4A	Q13619	777
DCAF7	CUL4B	Q13620	776
DCAF7	PHB2	Q99623	758
DCAF7	MAP3K1	Q13233	716
DCAF7	DYRK4	Q9NR20	699
DCAF7	HIPK2	Q9H2X6	692
DCAF7	P4HTM	Q9NXG6	651
DCAF7	RAD54L2	Q9Y4B4	617
DCAF7	YAF2	Q8IY57	578
DCAF7	LIN52	Q52LA3	559

IntAct

431 interactions, top by confidence:

A	B	Type	Score
CUL2	VHL	psi-mi:“MI:0914”(association)	0.940
NEDD8	UBE2M	psi-mi:“MI:0914”(association)	0.940
DYRK1A	DCAF7	psi-mi:“MI:0915”(physical association)	0.940
RYBP	CSNK2A2	psi-mi:“MI:0914”(association)	0.900
YWHAH	ABLIM1	psi-mi:“MI:0914”(association)	0.800
CTBP1	ZEB2	psi-mi:“MI:0914”(association)	0.800
PLK1	SPAG9	psi-mi:“MI:0914”(association)	0.790
CUL4B	COPS2	psi-mi:“MI:0914”(association)	0.790
TUBG1	TUBG1	psi-mi:“MI:2364”(proximity)	0.760
NS	PIK3R2	psi-mi:“MI:0914”(association)	0.750
rep	GTF2F2	psi-mi:“MI:0914”(association)	0.730
CFTR	ESYT2	psi-mi:“MI:2364”(proximity)	0.710
HTT	DCAF7	psi-mi:“MI:0915”(physical association)	0.670
DYRK1A	RB1	psi-mi:“MI:0914”(association)	0.670
YAF2	E2F6	psi-mi:“MI:0914”(association)	0.640
YWHAG	BLTP3B	psi-mi:“MI:0914”(association)	0.640
PARD3	PRKCI	psi-mi:“MI:0914”(association)	0.620
DCAF7	PFDN6	psi-mi:“MI:2364”(proximity)	0.570
DCAF7	PFDN6	psi-mi:“MI:0914”(association)	0.570
YWHAH	BLTP3B	psi-mi:“MI:0914”(association)	0.570

BioGRID (994): DCAF7 (Affinity Capture-MS), DCAF7 (Affinity Capture-MS), CCT2 (Affinity Capture-MS), TCP1 (Affinity Capture-MS), CCT3 (Affinity Capture-MS), CCT4 (Affinity Capture-MS), CCT5 (Affinity Capture-MS), CCT6A (Affinity Capture-MS), CCT7 (Affinity Capture-MS), CCT8 (Affinity Capture-MS), DYRK1A (Affinity Capture-MS), MAP3K1 (Affinity Capture-MS), TCP1 (Affinity Capture-Western), CCT2 (Affinity Capture-Western), DCAF7 (Affinity Capture-Western)

ESM2 similar proteins: A7YY75, C1BK83, O60907, O89050, P17427, P18484, P61962, P61963, P97834, Q05048, Q08211, Q1JP79, Q28141, Q28D01, Q4FZW5, Q4R8H1, Q58E77, Q5BJQ6, Q5R874, Q5R8K2, Q5RAN6, Q5RB35, Q5U4Y8, Q5ZHN3, Q5ZMV7, Q640J6, Q6GL39, Q6GNF1, Q6GPC6, Q6NV31, Q6TGU2, Q6UXN9, Q7SZM9, Q8BFQ4, Q8BHJ5, Q8C6G8, Q8R2U0, Q92747, Q96EE3, Q99LC2

Diamond homologs: O74763, P61962, P61963, Q12523, Q38960, Q552R1, Q5I0B9, Q676U5, Q9FN19, Q9LPV9, Q9XGN1, Q09028, Q3MHL3, Q5RF92, Q60972, Q9W7I5, F1LTR1, O14021, O22466, O22467, O22468, O22469, O22607, O93377, O94244, O94423, P0CS36, P0CS37, P26309, P39984, P90916, P90917, Q10G81, Q16576, Q24572, Q28D01, Q2UA71, Q2YDS1, Q3B7M6, Q3SWX8

SIGNOR signaling

3 interactions.

A	Effect	B	Mechanism
DCAF7	“down-regulates quantity by repression”	GLI1	“transcriptional regulation”
DCAF7	“up-regulates activity”	DYRK1A	binding
DCAF7	“up-regulates activity”	DYRK1B	binding

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 201 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
SARS-CoV-1 targets host intracellular signalling and regulatory pathways	5	24.2×	4e-04
RHO GTPases activate PKNs	5	11.4×	3e-03
RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known	5	10.8×	3e-03
Aggrephagy	6	10.7×	1e-03
Deactivation of the beta-catenin transactivating complex	6	10.1×	1e-03
Regulation of PLK1 Activity at G2/M Transition	10	9.1×	1e-04
Loss of Nlp from mitotic centrosomes	8	9.1×	4e-04
Loss of proteins required for interphase microtubule organization from the centrosome	8	9.1×	4e-04

GO biological processes:

GO term	Partners	Fold	FDR
mitotic spindle organization	7	10.9×	1e-03
G1/S transition of mitotic cell cycle	7	8.0×	6e-03
anatomical structure morphogenesis	8	6.4×	7e-03
microtubule cytoskeleton organization	9	6.2×	4e-03
protein phosphorylation	16	6.2×	1e-05
transcription by RNA polymerase II	11	4.4×	7e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

21 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	8
Likely benign	0
Benign	3

Top pathogenic / likely-pathogenic (0)

SpliceAI

1180 predictions. Top by Δscore:

Variant	Effect	Δscore
17:63550812:CAAG:C	donor_loss	1.0000
17:63550813:AAG:A	donor_loss	1.0000
17:63550816:G:GA	donor_loss	1.0000
17:63579819:TTGCA:T	acceptor_loss	1.0000
17:63579820:TGCA:T	acceptor_loss	1.0000
17:63579821:GCAG:G	acceptor_loss	1.0000
17:63579822:CAG:C	acceptor_loss	1.0000
17:63579823:A:AG	acceptor_gain	1.0000
17:63579824:G:GG	acceptor_gain	1.0000
17:63579939:AAGAG:A	donor_gain	1.0000
17:63579940:AGAG:A	donor_gain	1.0000
17:63579941:GAG:G	donor_gain	1.0000
17:63579941:GAGG:G	donor_gain	1.0000
17:63579942:AGGTA:A	donor_loss	1.0000
17:63579944:G:GG	donor_gain	1.0000
17:63579944:GTAA:G	donor_loss	1.0000
17:63579945:T:G	donor_loss	1.0000
17:63585324:TGCAG:T	donor_loss	1.0000
17:63585326:CAGG:C	donor_loss	1.0000
17:63585327:AGGTA:A	donor_loss	1.0000
17:63585328:GGT:G	donor_loss	1.0000
17:63585330:T:G	donor_loss	1.0000
17:63589165:GAGT:G	donor_gain	1.0000
17:63589167:GT:G	donor_gain	1.0000
17:63589169:GT:G	donor_gain	1.0000
17:63550796:TGGAG:T	donor_gain	0.9900
17:63550797:GGAGG:G	donor_gain	0.9900
17:63550800:G:GT	donor_gain	0.9900
17:63579331:CTTCA:C	acceptor_loss	0.9900
17:63579332:TTCA:T	acceptor_loss	0.9900

AlphaMissense

2258 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
17:63550747:T:A	W24R	1.000
17:63550747:T:C	W24R	1.000
17:63550781:C:A	A35E	1.000
17:63550786:G:C	G37R	1.000
17:63550787:G:A	G37D	1.000
17:63550789:A:C	S38R	1.000
17:63550790:G:T	S38I	1.000
17:63550791:C:A	S38R	1.000
17:63550791:C:G	S38R	1.000
17:63550812:C:A	N45K	1.000
17:63550812:C:G	N45K	1.000
17:63578471:T:A	V47D	1.000
17:63578530:C:G	H67D	1.000
17:63578536:T:C	Y69H	1.000
17:63578536:T:G	Y69D	1.000
17:63578540:C:A	P70H	1.000
17:63578557:T:A	W76R	1.000
17:63578557:T:C	W76R	1.000
17:63578597:C:A	A89E	1.000
17:63578600:C:A	T90K	1.000
17:63578602:A:C	S91R	1.000
17:63578604:C:A	S91R	1.000
17:63578604:C:G	S91R	1.000
17:63578605:G:C	G92R	1.000
17:63578606:G:A	G92D	1.000
17:63578608:G:C	D93H	1.000
17:63578609:A:T	D93V	1.000
17:63578615:T:A	L95H	1.000
17:63578615:T:C	L95P	1.000
17:63578617:C:A	R96S	1.000

dbSNP variants (sampled 300 via entrez): RS1000031491 (17:63548881 A>G), RS1000083419 (17:63549102 G>A), RS1000239143 (17:63573829 G>A), RS1000293113 (17:63586612 A>C), RS1000311420 (17:63586376 G>A,T), RS1000426288 (17:63580048 C>G), RS1000578174 (17:63572121 C>A,T), RS1000631864 (17:63572589 G>A), RS1000645707 (17:63584706 G>C), RS1000657073 (17:63585043 G>A), RS1000813091 (17:63560335 T>A,C), RS1000845777 (17:63580169 A>G), RS1000862050 (17:63554043 A>G), RS1000872464 (17:63549018 C>T), RS1000884815 (17:63592274 G>A)

Disease associations

OMIM: gene MIM:605973 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

2 associations (top):

Study	Trait	p-value
GCST000175_9	Height	7.000000e-07
GCST90020028_1438	Hip circumference adjusted for BMI	5.000000e-13

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0008039	BMI-adjusted hip circumference

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067213 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

4 potent at pChembl≥5 of 4 total, top 4 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
6.78	Kd	165.1	nM	CHEMBL5653589
6.74	ED50	184.2	nM	CHEMBL5653589
6.00	Kd	1006	nM	CHEMBL3752910
5.95	ED50	1123	nM	CHEMBL3752910

PubChem BioAssay actives

2 with measured affinity, of 4 total; 2 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2148199: Binding affinity to human DCAF7 incubated for 45 mins by Kinobead based pull down assay	kd	0.1651	uM
4-methyl-3-[(1-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2148199: Binding affinity to human DCAF7 incubated for 45 mins by Kinobead based pull down assay	kd	1.0064	uM

CTD chemical–gene interactions

36 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, increases expression, decreases expression	2
Arsenic	affects expression, affects methylation	2
Nickel	decreases expression, increases expression	2
Valproic Acid	decreases expression, affects expression	2
methylmercuric chloride	increases expression	1
triphenyl phosphate	affects expression	1
bisphenol A	affects cotreatment, increases methylation	1
salinomycin	decreases expression	1
trichostatin A	affects cotreatment, decreases expression	1
tetrahydropalmatine	decreases expression	1
sodium arsenite	increases expression	1
beta-methylcholine	affects expression	1
di-n-butylphosphoric acid	affects expression	1
torcetrapib	increases expression	1
belinostat	increases expression	1
ICG 001	decreases expression	1
dorsomorphin	affects cotreatment, decreases expression	1
eprenetapopt	affects expression, affects reaction	1
LDN 193189	affects cotreatment, increases expression	1
4-(4-((5-(4,5-dimethyl-2-nitrophenyl)-2-furanyl)methylene)-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-1-yl)benzoic acid	decreases expression	1
Fulvestrant	affects cotreatment, increases methylation	1
Vorinostat	decreases expression	1
Air Pollutants	affects expression, increases abundance	1
Air Pollutants, Occupational	decreases expression	1
Benzo(a)pyrene	increases methylation	1
Enzyme Inhibitors	decreases activity, increases O-linked glycosylation	1
Hydrogen Peroxide	affects expression	1
Ivermectin	decreases expression	1
Lead	affects methylation	1
Ozone	affects expression, increases abundance	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5651241	Binding	Binding affinity to human DCAF7 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.