DCANP1

Summary

DCANP1 (dendritic cell associated nuclear protein 1, HGNC:24459) is a protein-coding gene on chromosome 5q31.1, encoding Dendritic cell nuclear protein 1 (Q8TF63). Binds with and transactivates the corticotropin-releasing hormone (CRH) promoter.

This intronless gene is specifically expressed in dendritic cells (DCs), which are potent antigen-presenting cells involved in activating naive T cells to initiate antigen-specific immune response. The encoded protein is localized mainly in the perinucleus. One of the alleles (A/T) of this gene, that causes premature translation termination at aa 117, has been associated with an increased prevalence of major depression in humans.

Source: NCBI Gene 140947 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 45 total
• MANE Select transcript: NM_130848

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:24459
Approved symbol	DCANP1
Name	dendritic cell associated nuclear protein 1
Location	5q31.1
Locus type	gene with protein product
Status	Approved
Aliases	DCNP1
Ensembl gene	ENSG00000251380
Ensembl biotype	protein_coding
OMIM	609710
Entrez	140947

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 protein_coding

ENST00000503143

RefSeq mRNA: 1 — MANE Select: NM_130848 NM_130848

CCDS: CCDS4186

Canonical transcript exons

ENST00000503143 — 1 exons

Exon	Start	End
ENSE00002043461	135444214	135447348

Expression profiles

Bgee: expression breadth broad, 37 present calls, max score 88.75.

Top tissues by expression

114 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	88.75	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	77.58	silver quality
granulocyte	CL:0000094	62.92	gold quality
mucosa of transverse colon	UBERON:0004991	57.10	gold quality
bone marrow cell	CL:0002092	50.60	gold quality
lymph node	UBERON:0000029	49.28	gold quality
bone marrow	UBERON:0002371	46.34	silver quality
leukocyte	CL:0000738	46.01	gold quality
monocyte	CL:0000576	45.59	gold quality
spleen	UBERON:0002106	45.20	gold quality
colonic epithelium	UBERON:0000397	41.28	gold quality
blood	UBERON:0000178	40.34	gold quality
ventricular zone	UBERON:0003053	39.92	gold quality
skeletal muscle tissue	UBERON:0001134	39.40	gold quality
gall bladder	UBERON:0002110	38.07	gold quality
small intestine Peyer’s patch	UBERON:0003454	37.64	gold quality
muscle tissue	UBERON:0002385	37.62	gold quality
ganglionic eminence	UBERON:0004023	37.18	gold quality
lower esophagus mucosa	UBERON:0035834	37.04	gold quality
small intestine	UBERON:0002108	36.79	gold quality
cortical plate	UBERON:0005343	36.47	gold quality
prefrontal cortex	UBERON:0000451	35.81	gold quality
hindlimb stylopod muscle	UBERON:0004252	35.21	gold quality
rectum	UBERON:0001052	34.80	silver quality
placenta	UBERON:0001987	34.63	gold quality
skin of leg	UBERON:0001511	34.40	gold quality
zone of skin	UBERON:0000014	34.25	gold quality
skin of abdomen	UBERON:0001416	33.56	silver quality
transverse colon	UBERON:0001157	33.33	gold quality
esophagus mucosa	UBERON:0002469	33.32	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.58

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

68 targeting DCANP1, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-432-3P	100.00	67.86	705
HSA-MIR-12118	100.00	65.88	1270
HSA-MIR-6077	99.99	68.04	2299
HSA-MIR-6891-5P	99.98	66.53	1372
HSA-MIR-3173-3P	99.98	66.49	1217
HSA-MIR-23A-3P	99.95	74.24	3163
HSA-MIR-23B-3P	99.95	74.24	3163
HSA-MIR-23C	99.95	73.92	3192
HSA-MIR-4492	99.87	68.25	3611
HSA-MIR-3919	99.87	69.45	2489
HSA-MIR-3180-5P	99.82	69.12	2422
HSA-MIR-548AG	99.77	69.25	1492
HSA-MIR-4306	99.72	70.50	3630
HSA-MIR-548M	99.70	68.87	1749
HSA-MIR-548AI	99.69	69.24	1494
HSA-MIR-548BA	99.69	69.14	1514
HSA-MIR-570-5P	99.69	69.24	1494
HSA-MIR-4527	99.66	67.43	714
HSA-MIR-567	99.63	68.57	1219
HSA-MIR-4516	99.61	67.78	3390
HSA-MIR-7152-5P	99.60	69.33	2094
HSA-MIR-24-3P	99.59	69.97	1934
HSA-MIR-762	99.58	66.61	1994
HSA-MIR-4687-3P	99.48	66.41	968
HSA-MIR-4498	99.47	67.42	2360
HSA-MIR-122B-5P	99.46	70.81	1457
HSA-MIR-6722-3P	99.45	67.62	1919
HSA-MIR-4318	99.38	66.94	1505
HSA-MIR-520A-5P	99.35	66.72	1632
HSA-MIR-525-5P	99.35	66.85	1615

Literature-anchored findings (GeneRIF, showing 6)

• molecular cloning and immunocytochemical analysis (PMID:11798177)
• Marker rs12520799, in dendritic cell nuclear protein 1 on chromosome 5, yielded a significant association with lifetime major depression. (PMID:16189510)
• the genetic polymorphism of DCNP1 may influence production of specific IgE by altering DC (Dendritic Cells)functions in the mite allergen presenting and/or processing. (PMID:17460725)
• We propose that full-length dendritic cell nucleus protein-1 may play a role in the pathogenesis of depressive disorders by enhancing corticotropin-releasing hormone expression in the hypothalamic paraventricular nucleus. (PMID:20693543)
• In the Han Chinese population, common DCNP1 polymorphisms are unlikely to be important in the genetic susceptibility to MDD. (PMID:23619526)
• Our study reveals a connection between the major depression candidate protein DCNP1, circadian system and melatonin biosynthesis, which may contribute to the pathogenesis of depression. (PMID:29219947)

Cross-species orthologs

0 orthologs

Protein

Protein identifiers

Dendritic cell nuclear protein 1 — Q8TF63 (reviewed: Q8TF63)

Alternative names: Dendritic cell nuclear protein-1, Dendritic cell-associated nuclear protein

All UniProt accessions (1): Q8TF63

UniProt curated annotations — full annotation on UniProt →

Function. Binds with and transactivates the corticotropin-releasing hormone (CRH) promoter.

Subcellular location. Nucleus. Cytoplasm.

Tissue specificity. Expressed in neurons of the paraventricular nucleus, thalamus and occipital cortex and in glial cells (at protein level). Predominantly expressed in dendritic cells. Detected in brain and skeletal muscle. Highly expressed in mature dendritic cells and at a lower level in immature dendritic cells. Expressed in paraventricular nucleus, supraoptic nucleus and nucleus basalis of Meynert. Strongly expressed in paraventricular nucleus of depressed patients compared to controls. Not expressed in monocytes and B-cells.

RefSeq proteins (1): NP_570900* (*=MANE)

Domains & families (InterPro)

UniProt features (10 total): compositionally biased region 4, region of interest 3, sequence variant 2, chain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q8TF63-F1	36.60	0.00

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 121 (showing top): GOBP_DIGESTION, GOBP_COGNITION, GOBP_AXIS_SPECIFICATION, GOBP_BEHAVIOR, GOBP_SKELETAL_SYSTEM_DEVELOPMENT, MODULE_255, GOBP_EMBRYONIC_AXIS_SPECIFICATION, MODULE_317, GOBP_CRANIAL_NERVE_MORPHOGENESIS, GOBP_CRANIAL_NERVE_DEVELOPMENT, GOBP_NEUROMUSCULAR_PROCESS_CONTROLLING_BALANCE, GOBP_ANIMAL_ORGAN_MORPHOGENESIS, GOBP_BONE_DEVELOPMENT, GOBP_NEGATIVE_REGULATION_OF_MULTICELLULAR_ORGANISMAL_PROCESS, GOBP_NEGATIVE_REGULATION_OF_TRANSPORT

GO Biological Process (19): thorax and anterior abdomen determination (GO:0007356), trigeminal nerve development (GO:0021559), vestibulocochlear nerve formation (GO:0021650), peristalsis (GO:0030432), auditory behavior (GO:0031223), genitalia morphogenesis (GO:0035112), inner ear morphogenesis (GO:0042472), regulation of muscle organ development (GO:0048634), genitalia development (GO:0048806), inner ear development (GO:0048839), neuromuscular process controlling balance (GO:0050885), mastication (GO:0071626), cochlea development (GO:0090102), cochlea morphogenesis (GO:0090103), craniofacial suture morphogenesis (GO:0097094), learned vocalization behavior (GO:0098583), negative regulation of relaxation of muscle (GO:1901078), negative regulation of saliva secretion (GO:1905747), hard palate morphogenesis (GO:1905748)

GO Molecular Function (2): DNA binding (GO:0003677), protein binding (GO:0005515)

GO Cellular Component (4): nucleoplasm (GO:0005654), cytoplasm (GO:0005737), nuclear body (GO:0016604), nucleus (GO:0005634)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
embryonic morphogenesis	2
inner ear development	2
anatomical structure development	2
anatomical structure morphogenesis	2
cellular anatomical structure	2
zygotic determination of anterior/posterior axis, embryo	1
anterior/posterior pattern specification	1
cranial nerve development	1
cranial nerve formation	1
vestibulocochlear nerve morphogenesis	1
phasic smooth muscle contraction	1
mechanosensory behavior	1
response to auditory stimulus	1
developmental process involved in reproduction	1
animal organ morphogenesis	1
genitalia development	1
ear morphogenesis	1
muscle organ development	1
regulation of developmental process	1
sex differentiation	1
animal organ development	1
reproductive structure development	1
ear development	1
musculoskeletal movement	1
neuromuscular process	1
digestive system process	1
inner ear morphogenesis	1
cochlea development	1
bone morphogenesis	1
cranial skeletal system development	1
vocalization behavior	1
learned vocalization behavior or vocal learning	1
negative regulation of multicellular organismal process	1
relaxation of muscle	1
regulation of relaxation of muscle	1
saliva secretion	1
regulation of saliva secretion	1
negative regulation of secretion	1
negative regulation of digestive system process	1
nucleic acid binding	1

Protein interactions and networks

STRING

130 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
DCANP1	CD68	P34810	766
DCANP1	ERICH4	A6NGS2	511
DCANP1	TMED6	Q8WW62	348
DCANP1	PGBD5	Q8N414	348
DCANP1	TMPRSS9	Q7Z410	348
DCANP1	SLC38A10	Q9HBR0	306
DCANP1	TBC1D16	Q8TBP0	305
DCANP1	CCDC3	Q9BQI4	305
DCANP1	MRPS18B	Q9Y676	290
DCANP1	NPY	P01303	273
DCANP1	TIFAB	Q6ZNK6	271
DCANP1	MYH15	Q9Y2K3	265
DCANP1	LTV1	Q96GA3	257
DCANP1	TREML4	Q6UXN2	255
DCANP1	INPP5A	Q14642	248

IntAct

22 interactions, top by confidence:

A	B	Type	Score
DCANP1	MDFI	psi-mi:“MI:0915”(physical association)	0.780
MDFI	DCANP1	psi-mi:“MI:0915”(physical association)	0.780
BOLL	DCANP1	psi-mi:“MI:0915”(physical association)	0.560
DCANP1	KRTAP3-3	psi-mi:“MI:0915”(physical association)	0.560
DCANP1	DDX3X	psi-mi:“MI:0915”(physical association)	0.560
	DCANP1	psi-mi:“MI:0915”(physical association)	0.560
DCANP1	IDE	psi-mi:“MI:0914”(association)	0.350
DCANP1	BOLL	psi-mi:“MI:0915”(physical association)	0.000
DCANP1	MDFI	psi-mi:“MI:0915”(physical association)	0.000
DCANP1	KRTAP3-3	psi-mi:“MI:0915”(physical association)	0.000

BioGRID (19): DCANP1 (Two-hybrid), DCANP1 (Two-hybrid), DCANP1 (Two-hybrid), DCANP1 (Two-hybrid), FREM2 (Affinity Capture-MS), FAT1 (Affinity Capture-MS), FAT3 (Affinity Capture-MS), PCDHGA11 (Affinity Capture-MS), FAT4 (Affinity Capture-MS), CELSR2 (Affinity Capture-MS), CELSR3 (Affinity Capture-MS), PCDH20 (Affinity Capture-MS), IDE (Affinity Capture-MS), FZD3 (Affinity Capture-MS), CELSR1 (Affinity Capture-MS)

ESM2 similar proteins: A0A1B0GUT2, A0A3Q1LFG5, A1L4Q6, A2RUQ5, A8MQB3, A8MU10, A8MZ25, B1ANY3, C9JC47, P0C7V0, P0C864, P0CG42, P0CG43, P0DP75, P0DPA3, P0DXC1, P20975, P20977, P24026, P59020, P59021, P87743, Q06250, Q0IIN9, Q52M75, Q5SR53, Q5T036, Q5W150, Q67923, Q6ZUF6, Q6ZUU3, Q71F78, Q7Z4H9, Q8IYB0, Q8JMY5, Q8JMZ5, Q8JN06, Q8N2X6, Q8N5N4, Q8N9X3

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

45 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	39
Likely benign	6
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

407 predictions. Top by Δscore:

Variant	Effect	Δscore
5:135445862:T:C	acceptor_gain	0.9600
5:135444512:C:CC	acceptor_gain	0.9200
5:135444510:TG:T	acceptor_gain	0.9000
5:135446754:T:TA	donor_gain	0.8800
5:135444812:T:C	acceptor_gain	0.8500
5:135446623:AACTG:A	donor_gain	0.8500
5:135444695:T:TC	acceptor_gain	0.8400
5:135446444:ACCCG:A	donor_gain	0.8300
5:135446445:CCCGC:C	donor_gain	0.8300
5:135446624:A:C	donor_gain	0.8200
5:135445862:T:TC	acceptor_gain	0.8100
5:135447099:C:CT	acceptor_gain	0.7900
5:135444508:GATGC:G	acceptor_loss	0.7600
5:135444509:ATG:A	acceptor_loss	0.7600
5:135444511:GCTGA:G	acceptor_loss	0.7600
5:135444512:CTGAG:C	acceptor_loss	0.7600
5:135444513:T:C	acceptor_loss	0.7600
5:135445862:T:TG	acceptor_gain	0.7600
5:135444514:G:C	acceptor_loss	0.7400
5:135444884:ACT:A	donor_gain	0.7400
5:135444885:CTC:C	donor_gain	0.7400
5:135444507:GGATG:G	acceptor_gain	0.7300
5:135444508:GATG:G	acceptor_gain	0.7200
5:135445708:T:TA	donor_gain	0.7200
5:135444692:CGGT:C	acceptor_gain	0.7100
5:135444695:T:C	acceptor_gain	0.7000
5:135445007:C:CT	acceptor_gain	0.7000
5:135445861:CT:C	acceptor_gain	0.7000
5:135446448:G:A	donor_gain	0.6700
5:135446445:CCCG:C	donor_gain	0.6600

AlphaMissense

1559 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
5:135446509:G:C	F200L	0.942
5:135446509:G:T	F200L	0.942
5:135446511:A:G	F200L	0.942
5:135446701:G:C	F136L	0.928
5:135446701:G:T	F136L	0.928
5:135446703:A:G	F136L	0.928
5:135446632:A:C	F159L	0.925
5:135446632:A:T	F159L	0.925
5:135446634:A:G	F159L	0.925
5:135446845:G:C	F88L	0.893
5:135446845:G:T	F88L	0.893
5:135446847:A:G	F88L	0.893
5:135446629:C:A	K160N	0.886
5:135446629:C:G	K160N	0.886
5:135446686:G:C	F141L	0.862
5:135446686:G:T	F141L	0.862
5:135446688:A:G	F141L	0.862
5:135446500:C:A	W203C	0.831
5:135446500:C:G	W203C	0.831
5:135446502:A:G	W203R	0.821
5:135446502:A:T	W203R	0.821
5:135446702:A:G	F136S	0.742
5:135446630:T:A	K160M	0.739
5:135446407:G:C	S234R	0.708
5:135446407:G:T	S234R	0.708
5:135446409:T:G	S234R	0.708
5:135446506:C:A	R201S	0.708
5:135446506:C:G	R201S	0.708
5:135446587:A:C	S174R	0.691
5:135446587:A:T	S174R	0.691

dbSNP variants (sampled 300 via entrez): RS1000476115 (5:135447971 C>G), RS1000516626 (5:135446157 AC>A), RS1001559869 (5:135445565 C>A,G,T), RS1001893830 (5:135444594 C>G,T), RS1002367668 (5:135449199 C>A,T), RS1002412686 (5:135449003 C>T), RS1002580919 (5:135446638 T>A,C,G), RS1004116240 (5:135444308 A>C,G), RS1004961209 (5:135449185 C>T), RS1005400636 (5:135449034 A>G,T), RS1005743564 (5:135445362 T>C), RS1005875475 (5:135448476 T>G), RS10059928 (5:135445014 G>C,T), RS1006000519 (5:135448063 T>C), RS1006028332 (5:135444083 T>C)

Disease associations

OMIM: gene MIM:609710 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST007192_1	Coronary artery aneurysm in Kawasaki disease	2.000000e-07

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

7 total (human), top 7 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
(+)-JQ1 compound	decreases expression	1
Arsenic	affects methylation	1
Benzo(a)pyrene	affects methylation	1
Iron	decreases expression	1
Nickel	increases expression	1
Tobacco Smoke Pollution	decreases expression	1
Antirheumatic Agents	decreases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.