DCTPP1

Summary

DCTPP1 (dCTP pyrophosphatase 1, HGNC:28777) is a protein-coding gene on chromosome 16p11.2, encoding dCTP pyrophosphatase 1 (Q9H773). Hydrolyzes deoxynucleoside triphosphates (dNTPs) to the corresponding nucleoside monophosphates.

The protein encoded by this gene is dCTP pyrophosphatase, which converts dCTP to dCMP and inorganic pyrophosphate. The encoded protein also displays weak activity against dTTP and dATP, but none against dGTP. This protein may be responsible for eliminating excess dCTP after DNA synthesis and may prevent overmethylation of CpG islands. Three transcript variants, one protein-coding and the other two non-protein coding, have been found for this gene.

Source: NCBI Gene 79077 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 33 total
• Druggable target: yes — 4 molecules with ChEMBL bioactivity
• MANE Select transcript: NM_024096

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:28777
Approved symbol	DCTPP1
Name	dCTP pyrophosphatase 1
Location	16p11.2
Locus type	gene with protein product
Status	Approved
Aliases	MGC5627, RS21C6, CDA03, XTP3TPA
Ensembl gene	ENSG00000179958
Ensembl biotype	protein_coding
OMIM	615840
Entrez	79077

Gene structure

Transcript identifiers

Ensembl transcripts: 6 — 6 protein_coding

ENST00000319285, ENST00000565758, ENST00000567983, ENST00000568434, ENST00000568973, ENST00000678016

RefSeq mRNA: 1 — MANE Select: NM_024096 NM_024096

CCDS: CCDS10680

Canonical transcript exons

ENST00000319285 — 3 exons

Exon	Start	End
ENSE00001220331	30423615	30424533
ENSE00001220335	30429880	30430030
ENSE00003488830	30429057	30429167

Expression profiles

Bgee: expression breadth ubiquitous, 279 present calls, max score 97.54.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 38.5668 / max 284.8384, expressed in 1798 samples.

FANTOM5 promoters (4 alternative TSS)

Promoter ID	TPM avg	Samples expressed
157054	37.4268	1795
157053	1.1172	612
157052	0.0154	3
157051	0.0074	2

Top tissues by expression

286 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
mucosa of transverse colon	UBERON:0004991	97.54	gold quality
rectum	UBERON:0001052	95.39	gold quality
parotid gland	UBERON:0001831	95.11	gold quality
cervix squamous epithelium	UBERON:0006922	95.01	silver quality
oocyte	CL:0000023	94.64	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	94.53	gold quality
colonic mucosa	UBERON:0000317	94.46	gold quality
mucosa of sigmoid colon	UBERON:0004993	94.32	gold quality
endothelial cell	CL:0000115	93.88	gold quality
nasal cavity epithelium	UBERON:0005384	93.61	gold quality
hair follicle	UBERON:0002073	92.93	silver quality
pancreatic ductal cell	CL:0002079	92.58	silver quality
ganglionic eminence	UBERON:0004023	92.48	gold quality
embryo	UBERON:0000922	92.42	gold quality
gingival epithelium	UBERON:0001949	92.36	gold quality
transverse colon	UBERON:0001157	92.25	gold quality
nephron tubule	UBERON:0001231	92.20	gold quality
cortical plate	UBERON:0005343	92.20	gold quality
penis	UBERON:0000989	92.05	gold quality
islet of Langerhans	UBERON:0000006	91.91	gold quality
body of tongue	UBERON:0011876	91.76	gold quality
gingiva	UBERON:0001828	91.44	gold quality
epithelium of nasopharynx	UBERON:0001951	91.41	silver quality
pharyngeal mucosa	UBERON:0000355	91.37	gold quality
heart right ventricle	UBERON:0002080	91.26	gold quality
metanephric glomerulus	UBERON:0004736	91.26	gold quality
ventricular zone	UBERON:0003053	91.20	gold quality
ileal mucosa	UBERON:0000331	91.06	gold quality
pons	UBERON:0000988	91.05	gold quality
apex of heart	UBERON:0002098	91.03	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 2.

Experiment	Marker?	Max mean expression
E-CURD-112	yes	10.49
E-ANND-3	yes	7.57

Regulation

Is transcription factor: yes

Downstream targets (CollecTRI)

1 targets.

Target	Regulation
ABCB1	Activation

miRNA regulators (miRDB)

17 targeting DCTPP1, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-6721-5P	99.93	68.92	2981
HSA-MIR-6508-5P	99.92	70.67	2465
HSA-MIR-4319	99.76	69.83	2586
HSA-MIR-7161-5P	99.68	68.92	1592
HSA-MIR-548U	99.65	67.78	1463
HSA-MIR-26A-1-3P	99.64	66.81	788
HSA-MIR-26A-2-3P	99.64	66.82	786
HSA-MIR-4261	99.59	70.30	3415
HSA-MIR-4742-5P	98.89	68.41	1542
HSA-MIR-513B-3P	98.76	68.12	1577
HSA-MIR-6811-5P	97.98	64.96	848
HSA-MIR-3173-5P	97.35	65.82	1282
HSA-MIR-6799-3P	97.35	65.60	1302
HSA-MIR-4327	97.21	67.71	676
HSA-MIR-3192-5P	96.98	65.76	1926
HSA-MIR-4529-5P	96.74	65.77	569
HSA-MIR-193A-5P	95.70	65.33	613

Literature-anchored findings (GeneRIF, showing 8)

• Human dCTP pyrophosphatase 1 binds and is inhibited by the small molecule triptolide in vitro. (PMID:21671327)
• DCTPP1 has a central role in the balance of dCTP and the metabolism of deoxycytidine analogues. (PMID:24467396)
• Hypermethylation of CpG islands in MDR1 gene promoter region is obvious in DCTPP1-knockdown BGC-823 gastric cancer (GC) cells. (PMID:27612427)
• DCTPP1 may play an important role in PCa progression associated with high autophagy (PMID:29874556)
• Chemical Genetics Reveals a Role of dCTP Pyrophosphatase 1 in Wnt Signaling. (PMID:31173446)
• This study identifies a central role for DCTPP1 in the homeostasis of dCTP, dTTP and dUTP. (PMID:31377845)
• Silencing DSCAM-AS1 suppresses the growth and invasion of ER-positive breast cancer cells by downregulating both DCTPP1 and QPRT. (PMID:32716908)
• Expression of human dCTP pyrophosphatase 1 (DCTPP1) and its association with cisplatin resistance characteristics in ovarian cancer. (PMID:38686496)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	dctpp1	ENSDARG00000054334
mus_musculus	Dctpp1	ENSMUSG00000042462
rattus_norvegicus	Dctpp1	ENSRNOG00000017850

Protein

Protein identifiers

dCTP pyrophosphatase 1 — Q9H773 (reviewed: Q9H773)

Alternative names: Deoxycytidine-triphosphatase 1, RS21C6, XTP3-transactivated gene A protein

All UniProt accessions (3): Q9H773, H3BPN2, H3BSA6

UniProt curated annotations — full annotation on UniProt →

Function. Hydrolyzes deoxynucleoside triphosphates (dNTPs) to the corresponding nucleoside monophosphates. Has a strong preference for dCTP and its analogs including 5-iodo-dCTP and 5-methyl-dCTP for which it may even have a higher efficiency. May protect DNA or RNA against the incorporation of these genotoxic nucleotide analogs through their catabolism.

Subunit / interactions. Homotetramer.

Subcellular location. Mitochondrion. Nucleus. Cytoplasm. Cytosol.

Activity regulation. Inhibited by the reaction end product PPi. Inhibited by dCDP. Inhibited by triptolide.

Cofactor. Probably binds two or three Mg(2+) ions per subunit.

Induction. Up-regulated by an increase in cellular dCTP pool.

RefSeq proteins (1): NP_077001* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR025984	DCTPP	Family
IPR052555	dCTP_Pyrophosphatase	Family

Pfam: PF12643

Enzyme classification (BRENDA):

• EC 3.6.1.12 — dCTP diphosphatase (BRENDA: 5 organisms, 25 substrates, 173 inhibitors, 35 Km, 33 kcat entries)

Substrate kinetics (BRENDA)

15 substrates with measured Km, best-characterized 15. Km ranges are aggregated across organisms/conditions.

Substrate	Km (mM)	Measurements
DCTP	0.019–0.411	6
5-METHYL-DCTP	0.0213–0.156	5
5-IODO-DCTP	0.0039–0.239	4
5-BROMO-DCTP	0.0217–0.109	3
DATP	0.0793–0.293	3
DTTP	0.065–0.407	3
DUTP	0.24–0.484	2
2-CHLORO-DATP	0.107	1
2-HYDROXY-DATP	0.169	1
5-CHLORO-DCTP	0.05	1
5-FLUORO-DUTP	0.034	1
5-FORMYL-DCTP	0.0092	1
5-HYDROXY-DCTP	0.164	1
5-HYDROXYMETHYL-DCTP	0.0629	1
CTP	0.529	1

Catalyzed reactions (Rhea), 1 shown:

• dCTP + H2O = dCMP + diphosphate + H(+) (RHEA:22636)

UniProt features (23 total): binding site 8, helix 6, modified residue 4, region of interest 2, initiator methionine 1, chain 1, mutagenesis site 1

Structure

Experimental structures (PDB)

1 structures.

PDB	Method	Resolution (Å)
7MU5	X-RAY DIFFRACTION	2.2

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9H773-F1	85.40	0.71

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (8): 98; 102; 38; 47–51; 63; 66; 73; 95

Post-translational modifications (4): 2, 2, 12, 85

Mutagenesis-validated functional residues (1):

Position	Phenotype
63	loss of dctp diphosphatase activity.

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-499943	Interconversion of nucleotide di- and triphosphates

MSigDB gene sets: 243 (showing top): E2F_Q4, E2F_Q4_01, BUYTAERT_PHOTODYNAMIC_THERAPY_STRESS_DN, E2F4DP1_01, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_DN, GRAESSMANN_RESPONSE_TO_MC_AND_DOXORUBICIN_DN, GOBP_CARBOHYDRATE_DERIVATIVE_CATABOLIC_PROCESS, GOBP_ORGANOPHOSPHATE_METABOLIC_PROCESS, GOBP_CARBOHYDRATE_DERIVATIVE_METABOLIC_PROCESS, GOBP_NUCLEOBASE_CONTAINING_SMALL_MOLECULE_METABOLIC_PROCESS, GOBP_PYRIMIDINE_CONTAINING_COMPOUND_CATABOLIC_PROCESS, WEI_MYCN_TARGETS_WITH_E_BOX, MUELLER_PLURINET, GOBP_PYRIMIDINE_NUCLEOTIDE_METABOLIC_PROCESS, E2F1DP1_01

GO Biological Process (3): dCTP catabolic process (GO:0006253), nucleoside triphosphate catabolic process (GO:0009143), DNA protection (GO:0042262)

GO Molecular Function (10): magnesium ion binding (GO:0000287), pyrimidine deoxyribonucleotide binding (GO:0032556), identical protein binding (GO:0042802), nucleoside triphosphate diphosphatase activity (GO:0047429), dCTP diphosphatase activity (GO:0047840), nucleotide binding (GO:0000166), protein binding (GO:0005515), pyrophosphatase activity (GO:0016462), hydrolase activity (GO:0016787), metal ion binding (GO:0046872)

GO Cellular Component (5): nucleus (GO:0005634), nucleoplasm (GO:0005654), mitochondrion (GO:0005739), cytosol (GO:0005829), cytoplasm (GO:0005737)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
Metabolism of nucleotides	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	3
intracellular membrane-bounded organelle	2
cytoplasm	2
deoxyribonucleoside triphosphate catabolic process	1
pyrimidine deoxyribonucleoside triphosphate catabolic process	1
pyrimidine deoxyribonucleotide catabolic process	1
dCTP metabolic process	1
nucleoside triphosphate metabolic process	1
nucleoside phosphate catabolic process	1
DNA metabolic process	1
cellular response to stress	1
metal ion binding	1
pyrimidine nucleotide binding	1
carbohydrate derivative binding	1
protein binding	1
pyrophosphatase activity	1
nucleoside triphosphate diphosphatase activity	1
nucleoside phosphate binding	1
heterocyclic compound binding	1
binding	1
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	1
catalytic activity	1
cation binding	1
nuclear lumen	1
intracellular anatomical structure	1

Protein interactions and networks

STRING

1718 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
DCTPP1	DCTD	P32321	598
DCTPP1	LZIC	Q8WZA0	493
DCTPP1	ARPP19	P56211	469
DCTPP1	ITPA	Q9BY32	467
DCTPP1	ZNF354A	O60765	454
DCTPP1	UBAC2	Q8NBM4	435
DCTPP1	SNRPF	P62306	429
DCTPP1	CMTM3	Q96MX0	427
DCTPP1	RAB30	Q15771	418
DCTPP1	DUT	P33316	415
DCTPP1	ZNF841	Q6ZN19	413
DCTPP1	TADA3	O75528	402
DCTPP1	SETDB2	Q96T68	398
DCTPP1	DMAP1	Q9NPF5	398
DCTPP1	TM4SF1	P30408	395

IntAct

86 interactions, top by confidence:

A	B	Type	Score
DCTPP1	DCTPP1	psi-mi:“MI:0915”(physical association)	0.800
DCTPP1	NTAQ1	psi-mi:“MI:0915”(physical association)	0.740
NTAQ1	DCTPP1	psi-mi:“MI:0915”(physical association)	0.740
CFTR	ESYT2	psi-mi:“MI:0914”(association)	0.710
DCTPP1	CEP192	psi-mi:“MI:0915”(physical association)	0.590
SDCBP	DCTPP1	psi-mi:“MI:0915”(physical association)	0.560
DCTPP1	SDCBP	psi-mi:“MI:0915”(physical association)	0.560
GCD7	DCTPP1	psi-mi:“MI:0915”(physical association)	0.560
DCTPP1	PRE5	psi-mi:“MI:0915”(physical association)	0.560
DCTPP1	GCD7	psi-mi:“MI:0915”(physical association)	0.560
DCTPP1	DDIT4L	psi-mi:“MI:0915”(physical association)	0.560
DDIT4L	DCTPP1	psi-mi:“MI:0915”(physical association)	0.560
DCTPP1	SPG21	psi-mi:“MI:0915”(physical association)	0.560
LNX1	DCTPP1	psi-mi:“MI:0915”(physical association)	0.560
PSMA1	DCTPP1	psi-mi:“MI:0915”(physical association)	0.560
	NHERF1	psi-mi:“MI:0914”(association)	0.530
MPHOSPH6	ZFC3H1	psi-mi:“MI:0914”(association)	0.530
TUSC2	HSPA8	psi-mi:“MI:0914”(association)	0.530

BioGRID (81): DCTPP1 (Two-hybrid), DCTPP1 (Two-hybrid), DCTPP1 (Affinity Capture-RNA), DCTPP1 (Two-hybrid), DCTPP1 (Two-hybrid), DCTPP1 (Two-hybrid), DCTPP1 (Affinity Capture-MS), DCTPP1 (Affinity Capture-MS), DCTPP1 (Affinity Capture-MS), DCTPP1 (Affinity Capture-MS), DCTPP1 (Proximity Label-MS), DCTPP1 (Affinity Capture-MS), DCTPP1 (Affinity Capture-MS), DCTPP1 (Affinity Capture-MS), DCTPP1 (Affinity Capture-MS)

ESM2 similar proteins: A0A2L0V161, A0A7U3TBV3, A0PMQ6, A1TL08, A1W446, A1WFS9, A2S756, A2SE11, A5FYE6, B2HSB3, B8GKC9, C5CQK2, P0A5H2, P42979, P55389, P60539, P67443, P74755, P9WKW8, P9WKW9, P9WMC2, P9WMC3, P9WMU4, P9WMU5, Q12FC5, Q21U89, Q2KTS9, Q2RNA8, Q2SUB0, Q2YAV2, Q32KY6, Q3SWF2, Q50178, Q5FTN2, Q7NLB5, Q7TV28, Q7U635, Q7URL1, Q7VSY4, Q7W2X7

Diamond homologs: Q32KY6, Q91VC0, Q9H773, Q9QY93

SIGNOR signaling

4 interactions.

A	Effect	B	Mechanism
6-(3,4-Dimethoxyphenyl)-3-(furan-2-yl)-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole	“down-regulates activity”	DCTPP1	“chemical inhibition”
DCTPP1	up-regulates	Autophagy
DCTPP1	“up-regulates quantity by expression”	ABCB1	“transcriptional regulation”
DCTPP1	“up-regulates quantity by expression”	“dCMP 3’-end residue”	“chemical modification”

Disease & clinical

Clinical variants and AI predictions

ClinVar

33 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	30
Likely benign	3
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

418 predictions. Top by Δscore:

Variant	Effect	Δscore
16:30424530:CTGA:C	acceptor_gain	1.0000
16:30424531:TGA:T	acceptor_gain	1.0000
16:30424534:C:A	acceptor_loss	1.0000
16:30424534:C:CC	acceptor_gain	1.0000
16:30429081:T:TA	donor_gain	1.0000
16:30429082:C:A	donor_gain	1.0000
16:30429165:CGG:C	acceptor_gain	1.0000
16:30429168:C:CC	acceptor_gain	1.0000
16:30429874:GCTTA:G	donor_loss	1.0000
16:30429875:CTTA:C	donor_loss	1.0000
16:30429878:A:AC	donor_gain	1.0000
16:30429879:C:CT	donor_gain	1.0000
16:30429879:CATGT:C	donor_gain	1.0000
16:30424532:GA:G	acceptor_gain	0.9900
16:30424541:C:CT	acceptor_gain	0.9900
16:30424542:A:T	acceptor_gain	0.9900
16:30429051:ACT:A	donor_loss	0.9900
16:30429053:TCA:T	donor_loss	0.9900
16:30429054:C:CG	donor_loss	0.9900
16:30429055:A:AC	donor_gain	0.9900
16:30429055:A:C	donor_loss	0.9900
16:30429056:C:CC	donor_gain	0.9900
16:30429056:C:CG	donor_loss	0.9900
16:30429056:CA:C	donor_gain	0.9900
16:30429056:CAAG:C	donor_gain	0.9900
16:30429056:CAAGA:C	donor_gain	0.9900
16:30429163:GGCGG:G	acceptor_gain	0.9900
16:30429164:GCGG:G	acceptor_gain	0.9900
16:30429165:CGGC:C	acceptor_gain	0.9900
16:30429166:GG:G	acceptor_gain	0.9900

AlphaMissense

1089 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
16:30424384:T:A	K121I	0.994
16:30429146:A:C	F41L	0.994
16:30429146:A:T	F41L	0.994
16:30429148:A:G	F41L	0.994
16:30424383:T:A	K121N	0.990
16:30424383:T:G	K121N	0.990
16:30429119:G:C	F50L	0.990
16:30429119:G:T	F50L	0.990
16:30429121:A:G	F50L	0.990
16:30429128:C:A	W47C	0.985
16:30429128:C:G	W47C	0.985
16:30424527:C:A	W73C	0.982
16:30424527:C:G	W73C	0.982
16:30429147:A:C	F41C	0.982
16:30424453:T:A	D98V	0.981
16:30424452:G:C	D98E	0.980
16:30424452:G:T	D98E	0.980
16:30424455:A:C	S97R	0.979
16:30424455:A:T	S97R	0.979
16:30424457:T:G	S97R	0.979
16:30424529:A:G	W73R	0.978
16:30424529:A:T	W73R	0.978
16:30429147:A:G	F41S	0.975
16:30424456:C:A	S97I	0.974
16:30429906:G:C	F25L	0.974
16:30429906:G:T	F25L	0.974
16:30429908:A:G	F25L	0.974
16:30429067:C:G	A68P	0.971
16:30424533:A:C	F71L	0.970
16:30424533:A:T	F71L	0.970

dbSNP variants (sampled 300 via entrez): RS1000009593 (16:30431556 G>A), RS1000158976 (16:30427911 G>A), RS1000478071 (16:30429580 C>T), RS1000712503 (16:30427551 C>T), RS1001386470 (16:30428276 C>T), RS1002024258 (16:30428532 G>A), RS1002791841 (16:30426324 T>A,C), RS1003292693 (16:30430030 C>T), RS1003546988 (16:30425972 C>T), RS1003817896 (16:30425482 C>T), RS1004216245 (16:30430127 T>C), RS1004459671 (16:30430278 G>A,C,T), RS1004593524 (16:30423400 A>C), RS1004915681 (16:30425762 A>C), RS1005893446 (16:30431594 G>A,C)

Disease associations

OMIM: gene MIM:615840 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST008647_28	Urinary sodium excretion	5.000000e-12

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0009282	sodium measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL3769292 (SINGLE PROTEIN)

Molecules with ChEMBL bioactivity

4 molecules (phase ≥1), by development phase (incl. off-target/promiscuous compounds). Patent mentions across the top 20 by phase: 111,440 (via chembl_molecule»patent_compound — counts attach to the compound, not the gene–compound relationship, so off-target/promiscuous molecules can dominate).

Molecule	Name	Phase	Patents
CHEMBL553	ERLOTINIB	4	108,300
CHEMBL463763	TRIPTOLIDE	3	258
CHEMBL564829	MILCICLIB	2	821
CHEMBL494089	GSK-690693	1	2,061

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

180 potent at pChembl≥5 of 186 total, top 50 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
8.00	IC50	10	nM	TRIPTOLIDE
7.72	IC50	19	nM	CHEMBL3771346
7.72	IC50	19	nM	CHEMBL1578365
7.72	IC50	19	nM	CHEMBL4072033
7.70	IC50	20	nM	CHEMBL4065181
7.68	IC50	21	nM	CHEMBL3771039
7.60	IC50	25	nM	CHEMBL4082508
7.57	IC50	27	nM	CHEMBL3769835
7.54	Kd	28.53	nM	CHEMBL5653589
7.52	ED50	30.47	nM	CHEMBL5653589
7.47	IC50	34	nM	CHEMBL3769727
7.47	IC50	34	nM	CHEMBL4060634
7.44	IC50	36	nM	CHEMBL4075978
7.42	IC50	38	nM	CHEMBL4101470
7.39	IC50	41	nM	CHEMBL3771227
7.39	IC50	41	nM	CHEMBL4091797
7.34	IC50	46	nM	CHEMBL3771237
7.34	IC50	46	nM	CHEMBL3770446
7.33	IC50	47	nM	CHEMBL3771105
7.31	IC50	49	nM	CHEMBL3769687
7.29	IC50	51	nM	CHEMBL4098065
7.24	IC50	57	nM	CHEMBL3771138
7.19	IC50	65	nM	CHEMBL4061657
7.14	IC50	73	nM	CHEMBL3769653
7.13	IC50	74	nM	CHEMBL3770789
7.12	IC50	75	nM	CHEMBL3770659
7.10	IC50	79	nM	CHEMBL1519124
7.09	IC50	82	nM	CHEMBL3770096
7.07	IC50	85	nM	CHEMBL3770930
7.02	IC50	95	nM	CHEMBL3770808
7.02	IC50	96	nM	CHEMBL3770403
7.02	IC50	95	nM	CHEMBL4065981
6.88	IC50	133	nM	CHEMBL3770193
6.84	IC50	144	nM	CHEMBL4078660
6.79	IC50	164	nM	CHEMBL4086793
6.78	IC50	165	nM	CHEMBL3770701
6.77	IC50	170	nM	CHEMBL4086506
6.77	IC50	170	nM	CHEMBL4065129
6.75	IC50	177	nM	CHEMBL4065061
6.73	IC50	185	nM	CHEMBL3770766
6.72	IC50	190	nM	CHEMBL3770307
6.72	IC50	190	nM	CHEMBL4095068
6.72	IC50	190	nM	CHEMBL4060941
6.70	IC50	200	nM	CHEMBL4062129
6.69	IC50	205	nM	CHEMBL3771117
6.69	IC50	206	nM	CHEMBL3771148
6.69	IC50	204	nM	CHEMBL4104690
6.69	IC50	204	nM	CHEMBL4102676
6.68	IC50	208	nM	CHEMBL4066177
6.66	IC50	220	nM	CHEMBL4069727

PubChem BioAssay actives

178 with measured affinity, of 576 total; 50 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
(1S,2S,4S,5S,7R,8R,9S,11S,13S)-8-hydroxy-1-methyl-7-propan-2-yl-3,6,10,16-tetraoxaheptacyclo[11.7.0.02,4.02,9.05,7.09,11.014,18]icos-14(18)-en-17-one	2075675: Inhibition of DCTPP1 (unknown origin)	ic50	0.0100	uM
5,6-dichloro-2-methyl-1-[(4-methylphenyl)methyl]-4-nitrobenzimidazole	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.0190	uM
6-[4-(3-chloro-2-methylphenyl)sulfonylpiperazin-1-yl]-N-(thiophen-2-ylmethyl)pyridazine-3-carboxamide	1448206: Inhibition of human full length N-terminal His-tagged dCTPase expressed in Escherichia coli BL21(DE3) using dCTP as substrate by HTS-based malachite green assay	ic50	0.0190	uM
6-(3,4-dimethoxyphenyl)-3-(furan-2-yl)-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole	1440777: Inhibition of recombinant human N-terminal His-tagged dCTPase 1 expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by malachite green reagent based assay	ic50	0.0190	uM
6-(3,4-dimethoxyphenyl)-3-thiophen-2-yl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole	1440777: Inhibition of recombinant human N-terminal His-tagged dCTPase 1 expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by malachite green reagent based assay	ic50	0.0200	uM
4-[(5,6-dichloro-2-methyl-4-nitrobenzimidazol-1-yl)methyl]phenol	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.0210	uM
6-[4-[(2-chloro-3-pyridinyl)sulfonyl]piperazin-1-yl]-N-[(4-fluorophenyl)methyl]pyridazine-3-carboxamide	1448206: Inhibition of human full length N-terminal His-tagged dCTPase expressed in Escherichia coli BL21(DE3) using dCTP as substrate by HTS-based malachite green assay	ic50	0.0250	uM
[4-[(5,6-dichloro-2-cyclopropyl-4-nitrobenzimidazol-1-yl)methyl]phenyl]boronic acid	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.0270	uM
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2148205: Binding affinity to human DCTPP1 incubated for 45 mins by Kinobead based pull down assay	kd	0.0285	uM
[4-[(5,6-dichloro-2-methyl-4-nitrobenzimidazol-1-yl)methyl]phenyl] acetate	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.0340	uM
6-[4-(3-chloro-2-methylphenyl)sulfonylpiperazin-1-yl]-N-prop-2-ynylpyridazine-3-carboxamide	1448206: Inhibition of human full length N-terminal His-tagged dCTPase expressed in Escherichia coli BL21(DE3) using dCTP as substrate by HTS-based malachite green assay	ic50	0.0340	uM
N-[(4-fluorophenyl)methyl]-6-[4-[(2-methyl-3-pyridinyl)sulfonyl]piperazin-1-yl]pyridazine-3-carboxamide	1448206: Inhibition of human full length N-terminal His-tagged dCTPase expressed in Escherichia coli BL21(DE3) using dCTP as substrate by HTS-based malachite green assay	ic50	0.0360	uM
benzyl 6-[4-(2-methylphenyl)sulfonylpiperazin-1-yl]pyridazine-3-carboxylate	1448206: Inhibition of human full length N-terminal His-tagged dCTPase expressed in Escherichia coli BL21(DE3) using dCTP as substrate by HTS-based malachite green assay	ic50	0.0380	uM
5,6-dichloro-1-[(4-methoxyphenyl)methyl]-2-methyl-4-nitrobenzimidazole	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.0410	uM
prop-2-ynyl 6-[4-[2-(4-hydroxypiperidin-1-yl)phenyl]sulfonylpiperazin-1-yl]pyridazine-3-carboxylate	1448206: Inhibition of human full length N-terminal His-tagged dCTPase expressed in Escherichia coli BL21(DE3) using dCTP as substrate by HTS-based malachite green assay	ic50	0.0410	uM
[3-[(5,6-dichloro-2-methyl-4-nitrobenzimidazol-1-yl)methyl]phenyl]boronic acid	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.0460	uM
[4-[(5,6-dichloro-2-methyl-4-nitrobenzimidazol-1-yl)methyl]phenyl]boronic acid	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.0460	uM
2-[4-[(5,6-dichloro-2-methyl-4-nitrobenzimidazol-1-yl)methyl]phenyl]-6-methyl-1,3,6,2-dioxazaborocane-4,8-dione	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.0470	uM
1-benzyl-5,6-dichloro-2-methyl-4-nitrobenzimidazole	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.0490	uM
6-[4-(2-methylphenyl)sulfonylpiperazin-1-yl]-N-(thiophen-2-ylmethyl)pyridazine-3-carboxamide	1448206: Inhibition of human full length N-terminal His-tagged dCTPase expressed in Escherichia coli BL21(DE3) using dCTP as substrate by HTS-based malachite green assay	ic50	0.0510	uM
1-[(4-methoxyphenyl)methyl]-2,5,6-trimethyl-4-nitrobenzimidazole	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.0570	uM
N-[(4-fluorophenyl)methyl]-6-[4-(2-methylphenyl)sulfonylpiperazin-1-yl]pyridazine-3-carboxamide	1448206: Inhibition of human full length N-terminal His-tagged dCTPase expressed in Escherichia coli BL21(DE3) using dCTP as substrate by HTS-based malachite green assay	ic50	0.0650	uM
4-[(5,6-dichloro-2-methyl-4-nitrobenzimidazol-1-yl)methyl]benzoic acid	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.0730	uM
1-(benzenesulfonyl)-5,6-dichloro-2-methyl-4-nitrobenzimidazole	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.0740	uM
5,6-dichloro-2-methyl-4-nitro-1-[(4-pyrazol-1-ylphenyl)methyl]benzimidazole	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.0750	uM
6-(3,4-dimethoxyphenyl)-3-pyridin-2-yl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole	1440777: Inhibition of recombinant human N-terminal His-tagged dCTPase 1 expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by malachite green reagent based assay	ic50	0.0790	uM
[2-[(5,6-dichloro-2-methyl-4-nitrobenzimidazol-1-yl)methyl]phenyl]boronic acid	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.0820	uM
5,6-dichloro-2-methyl-4-nitro-1-phenylbenzimidazole	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.0850	uM
methyl 4-[(5,6-dichloro-2-methyl-4-nitrobenzimidazol-1-yl)methyl]benzoate	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.0950	uM
6-naphthalen-2-yl-3-pyridin-2-yl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole	1440777: Inhibition of recombinant human N-terminal His-tagged dCTPase 1 expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by malachite green reagent based assay	ic50	0.0950	uM
(5,6-dichloro-2-methyl-4-nitrobenzimidazol-1-yl)-phenylmethanone	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.0960	uM
4-[(5,6-dichloro-2-methyl-4-nitrobenzimidazol-1-yl)methyl]benzaldehyde	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.1330	uM
3-(furan-2-yl)-6-(4,5,6,7-tetrahydro-1-benzothiophen-3-yl)-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole	1440777: Inhibition of recombinant human N-terminal His-tagged dCTPase 1 expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by malachite green reagent based assay	ic50	0.1440	uM
6-(4-bromophenyl)-3-pyridin-2-yl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole	1440777: Inhibition of recombinant human N-terminal His-tagged dCTPase 1 expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by malachite green reagent based assay	ic50	0.1640	uM
4-[(5,6-dichloro-2-methyl-4-nitrobenzimidazol-1-yl)methyl]benzonitrile	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.1650	uM
N-benzyl-6-[4-(2-methylphenyl)sulfonylpiperazin-1-yl]pyridazine-3-carboxamide	1448206: Inhibition of human full length N-terminal His-tagged dCTPase expressed in Escherichia coli BL21(DE3) using dCTP as substrate by HTS-based malachite green assay	ic50	0.1700	uM
6-pyrazin-2-yl-3-thiophen-2-yl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole	1440777: Inhibition of recombinant human N-terminal His-tagged dCTPase 1 expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by malachite green reagent based assay	ic50	0.1700	uM
3-(furan-2-yl)-6-quinolin-2-yl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole	1440777: Inhibition of recombinant human N-terminal His-tagged dCTPase 1 expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by malachite green reagent based assay	ic50	0.1770	uM
5,6-dichloro-1-[(6-chloro-3-pyridinyl)methyl]-2-methyl-4-nitrobenzimidazole	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.1850	uM
5,6-dichloro-1-[2-(3,5-dimethyl-1H-pyrazol-4-yl)ethyl]-2-methyl-4-nitrobenzimidazole	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.1900	uM
6-(1,3-benzodioxol-5-yl)-3-pyridin-2-yl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole	1440777: Inhibition of recombinant human N-terminal His-tagged dCTPase 1 expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by malachite green reagent based assay	ic50	0.1900	uM
3-(4-chlorophenyl)-2,5-dimethyl-6-propyl-1H-pyrazolo[1,5-a]pyrimidin-7-one	1461534: Inhibition of full-length recombinant human dCTPase at by microtiter plate-adapted malachite green assay	ic50	0.1900	uM
4,7,8-trimethyl-6-[(4-methylphenyl)methyl]purino[7,8-a]imidazole-1,3-dione	1461534: Inhibition of full-length recombinant human dCTPase at by microtiter plate-adapted malachite green assay	ic50	0.2000	uM
6-naphthalen-1-yl-3-pyridin-2-yl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole	1440777: Inhibition of recombinant human N-terminal His-tagged dCTPase 1 expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by malachite green reagent based assay	ic50	0.2040	uM
6-(4-chlorophenyl)-3-pyridin-2-yl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole	1440777: Inhibition of recombinant human N-terminal His-tagged dCTPase 1 expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by malachite green reagent based assay	ic50	0.2040	uM
[4-[2-(5,6-dichloro-2-methyl-4-nitrobenzimidazol-1-yl)ethyl]phenyl]boronic acid	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.2050	uM
[4-[[4-nitro-2-(trifluoromethyl)benzimidazol-1-yl]methyl]phenyl]boronic acid	1281050: Inhibition of human recombinant full length dCTPase expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by HTS-adapted Malachite Green assay	ic50	0.2060	uM
3-(2-fluorophenyl)-6-(4-methoxyphenyl)-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole	1440777: Inhibition of recombinant human N-terminal His-tagged dCTPase 1 expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by malachite green reagent based assay	ic50	0.2080	uM
6-[(E)-3-(4-chlorophenyl)prop-2-enyl]-3-pyridin-2-yl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole	1440777: Inhibition of recombinant human N-terminal His-tagged dCTPase 1 expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by malachite green reagent based assay	ic50	0.2200	uM
6-(3,4-dimethylphenyl)-3-pyridin-2-yl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole	1440777: Inhibition of recombinant human N-terminal His-tagged dCTPase 1 expressed in Escherichia coli BL21(DE3)pLysS using dCTP as substrate after 1 hr by malachite green reagent based assay	ic50	0.2270	uM

CTD chemical–gene interactions

60 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
sodium arsenite	increases abundance, increases expression, decreases expression, affects cotreatment	4
Valproic Acid	affects cotreatment, increases expression, affects expression	4
bisphenol A	affects expression, decreases expression	2
(+)-JQ1 compound	decreases expression	2
Tobacco Smoke Pollution	affects expression, decreases expression	2
Particulate Matter	decreases expression, increases abundance	2
GSK-J4	decreases expression	1
cinobufagin	decreases expression	1
arsenite	affects binding, increases reaction	1
tris(1,3-dichloro-2-propyl)phosphate	decreases expression	1
cobaltous chloride	decreases expression	1
zinc chromate	decreases expression, increases abundance	1
manganese chloride	affects cotreatment, increases abundance, increases expression	1
nickel sulfate	decreases expression	1
di-n-butylphosphoric acid	affects expression	1
chromium hexavalent ion	decreases expression, increases abundance	1
CGP 52608	affects binding, increases reaction	1
chloropicrin	decreases expression	1
K 7174	decreases expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, increases expression	1
erucylphospho-N,N,N-trimethylpropylammonium	decreases expression	1
dorsomorphin	increases expression, affects cotreatment	1
(4-amino-1,4-dihydro-3-(2-pyridyl)-5-thioxo-1,2,4-triazole)copper(II)	decreases expression	1
2-(1H-indazol-4-yl)-6-(4-methanesulfonylpiperazin-1-ylmethyl)-4-morpholin-4-ylthieno(3,2-d)pyrimidine	decreases expression, increases response to substance	1
NSC 689534	affects binding, decreases expression	1
MT19c compound	decreases expression	1
Temozolomide	increases expression	1
Sunitinib	decreases expression	1
Acetaminophen	decreases expression	1
Air Pollutants	decreases expression, increases abundance	1

ChEMBL screening assays

30 unique, capped per target: 30 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL3772452	Binding	Reversible inhibition of His-tagged recombinant human dCTPase using dCTP as substrate assessed as remaining activity at 10 times IC50 preincubated for 1 hr followed by 100 fold dilution with substrate measured after 10 to 15 mins by jump di	Discovery of the First Potent and Selective Inhibitors of Human dCTP Pyrophosphatase 1. — J Med Chem

Cellosaurus cell lines

3 cell lines: 2 cancer cell line, 1 transformed cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B2VR	Abcam HEK293T DCTPP1 KO	Transformed cell line	Female
CVCL_SK51	HAP1 DCTPP1 (-) 1	Cancer cell line	Male
CVCL_SK52	HAP1 DCTPP1 (-) 2	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.