DCUN1D4

Summary

DCUN1D4 (defective in cullin neddylation 1 domain containing 4, HGNC:28998) is a protein-coding gene on chromosome 4q12, encoding DCN1-like protein 4 (Q92564). Contributes to the neddylation of all cullins by transferring NEDD8 from N-terminally acetylated NEDD8-conjugating E2s enzyme to different cullin C-terminal domain-RBX complexes which are necessary for the activation of cullin-RING E3 ubiquitin ligases (CRLs).

Enables cullin family protein binding activity. Involved in positive regulation of protein neddylation. Located in nucleus.

Source: NCBI Gene 23142 — RefSeq curated summary.

At a glance

• GWAS associations: 4
• Clinical variants (ClinVar): 38 total
• Druggable target: yes
• MANE Select transcript: NM_001040402

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:28998
Approved symbol	DCUN1D4
Name	defective in cullin neddylation 1 domain containing 4
Location	4q12
Locus type	gene with protein product
Status	Approved
Aliases	KIAA0276
Ensembl gene	ENSG00000109184
Ensembl biotype	protein_coding
OMIM	612977
Entrez	23142

Gene structure

Transcript identifiers

Ensembl transcripts: 28 — 11 protein_coding, 7 nonsense_mediated_decay, 7 protein_coding_CDS_not_defined, 3 retained_intron

ENST00000334635, ENST00000381441, ENST00000451288, ENST00000477560, ENST00000502930, ENST00000504113, ENST00000504661, ENST00000504923, ENST00000505403, ENST00000505634, ENST00000506673, ENST00000507659, ENST00000507741, ENST00000508257, ENST00000509068, ENST00000509376, ENST00000510518, ENST00000510587, ENST00000510808, ENST00000511675, ENST00000512199, ENST00000513800, ENST00000893398, ENST00000893399, ENST00000927742, ENST00000927743, ENST00000960096, ENST00000960097

RefSeq mRNA: 4 — MANE Select: NM_001040402 NM_001040402, NM_001287755, NM_001287757, NM_015115

CCDS: CCDS33982, CCDS3487, CCDS75123

Canonical transcript exons

ENST00000334635 — 11 exons

Exon	Start	End
ENSE00003503148	51863670	51863709
ENSE00003508164	51874271	51874385
ENSE00003522712	51913529	51916837
ENSE00003523283	51886568	51886638
ENSE00003577884	51913290	51913392
ENSE00003587175	51843153	51843267
ENSE00003611116	51899270	51899378
ENSE00003648100	51877763	51877854
ENSE00003651554	51863437	51863507
ENSE00003681637	51911070	51911174
ENSE00003789645	51891760	51891851

Expression profiles

Bgee: expression breadth ubiquitous, 290 present calls, max score 98.11.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 25.7649 / max 169.1686, expressed in 1807 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
47604	25.7649	1807

Top tissues by expression

295 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
calcaneal tendon	UBERON:0003701	98.11	gold quality
colonic epithelium	UBERON:0000397	95.44	gold quality
germinal epithelium of ovary	UBERON:0001304	94.62	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	93.64	gold quality
left ovary	UBERON:0002119	93.35	gold quality
right ovary	UBERON:0002118	93.12	gold quality
cauda epididymis	UBERON:0004360	93.05	gold quality
mucosa of stomach	UBERON:0001199	92.88	gold quality
popliteal artery	UBERON:0002250	92.88	gold quality
tibial artery	UBERON:0007610	92.87	gold quality
stromal cell of endometrium	CL:0002255	92.68	gold quality
blood vessel layer	UBERON:0004797	92.55	gold quality
lower esophagus muscularis layer	UBERON:0035833	92.55	gold quality
descending thoracic aorta	UBERON:0002345	92.53	gold quality
lower esophagus	UBERON:0013473	92.53	gold quality
right adrenal gland cortex	UBERON:0035827	92.43	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	92.41	gold quality
tendon	UBERON:0000043	92.40	gold quality
ovary	UBERON:0000992	92.40	gold quality
aorta	UBERON:0000947	92.39	gold quality
left testis	UBERON:0004533	92.29	gold quality
right adrenal gland	UBERON:0001233	92.05	gold quality
right testis	UBERON:0004534	92.05	gold quality
urinary bladder	UBERON:0001255	92.01	gold quality
testis	UBERON:0000473	91.95	gold quality
right coronary artery	UBERON:0001625	91.95	gold quality
adenohypophysis	UBERON:0002196	91.94	gold quality
tibial nerve	UBERON:0001323	91.91	gold quality
left adrenal gland cortex	UBERON:0035825	91.89	gold quality
sperm	CL:0000019	91.88	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-MTAB-6379	no	1316.61
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

289 targeting DCUN1D4, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-LET-7A-3P	100.00	74.03	3932
HSA-LET-7B-3P	100.00	74.08	3913
HSA-LET-7F-1-3P	100.00	74.02	3928
HSA-MIR-98-3P	100.00	74.08	3907
HSA-MIR-513A-5P	100.00	69.77	2465
HSA-MIR-3064-3P	100.00	70.09	1254
HSA-MIR-3613-3P	100.00	76.36	7965
HSA-MIR-5692A	100.00	74.40	6850
HSA-MIR-29A-3P	100.00	73.11	1835
HSA-MIR-29B-3P	100.00	73.18	1833
HSA-MIR-29C-3P	100.00	73.15	1833
HSA-MIR-9-5P	100.00	72.28	2361
HSA-MIR-3163	100.00	77.23	8605
HSA-MIR-3689D	100.00	66.14	1181
HSA-MIR-6851-5P	100.00	65.63	1294
HSA-MIR-3162-3P	100.00	65.37	363
HSA-MIR-4789-3P	99.99	70.75	2484
HSA-MIR-186-5P	99.99	70.83	3707
HSA-MIR-4282	99.99	75.36	6408
HSA-MIR-548AW	99.99	72.57	3559
HSA-MIR-4789-5P	99.98	70.76	2721
HSA-MIR-27A-3P	99.98	72.13	2955
HSA-MIR-27B-3P	99.98	72.13	2955
HSA-MIR-9985	99.98	72.11	2939
HSA-LET-7F-2-3P	99.98	70.98	2588
HSA-MIR-1185-1-3P	99.98	71.04	2593
HSA-MIR-1185-2-3P	99.98	71.04	2593
HSA-MIR-4775	99.98	75.00	6394
HSA-MIR-12136	99.98	72.81	5713
HSA-MIR-548P	99.98	72.25	3784

Cross-species orthologs

6 orthologs

Organism	Symbol	Gene ID
danio_rerio	dcun1d4	ENSDARG00000092394
mus_musculus	Dcun1d4	ENSMUSG00000051674
rattus_norvegicus	Dcun1d4	ENSRNOG00000002152
drosophila_melanogaster	SCCRO	FBGN0036510
drosophila_melanogaster	SCCRO4	FBGN0036967
caenorhabditis_elegans		WBGENE00010428

Paralogs (4): DCUN1D1 (ENSG00000043093), DCUN1D5 (ENSG00000137692), DCUN1D2 (ENSG00000150401), DCUN1D3 (ENSG00000188215)

Protein

Protein identifiers

DCN1-like protein 4 — Q92564 (reviewed: Q92564)

Alternative names: DCUN1 domain-containing protein 4, Defective in cullin neddylation protein 1-like protein 4

All UniProt accessions (9): Q92564, D6RB33, D6RCB7, D6RDV8, D6RGX7, D6RIY3, H0Y907, H0Y9C5, H0YA35

UniProt curated annotations — full annotation on UniProt →

Function. Contributes to the neddylation of all cullins by transferring NEDD8 from N-terminally acetylated NEDD8-conjugating E2s enzyme to different cullin C-terminal domain-RBX complexes which are necessary for the activation of cullin-RING E3 ubiquitin ligases (CRLs).

Subunit / interactions. Interacts (via the DCUN1 domain) with the unneddylated cullins: interacts with CUL1, CUL2, CUL3, CUL4A, CUL4B and CUL5; these interactions promote the cullin neddylation and the identity of the cullin dictates the affinity of the interaction. Interacts with RBX1 and RNF7. Interacts with CAND1; this interaction is bridged by cullins such as CUL3 and strongly inhibits the neddylation of CUL3. These CAND-cullin-DCNL complexes can only be neddylated in the presence of a substrate adapter. Interacts (via DCUN1 domain) with UBE2M (N-terminally acetylated form) and probably with UBE2F (N-terminally acetylated form).

Subcellular location. Nucleus.

Domain organisation. The DCUN1 domain, also known as PONY domain, mediates the interaction with different cullins. The DCUN1 domain mediates the interaction with the N-terminally acetylated NEDD8-conjugating E2s enzyme leading to the NEDD8 transfer from N-terminally acetylated NEDD8-conjugating E2s enzyme to different cullin C-terminal domain-RBX complexes; the neddylation efficiency correlates with the DCUN1D5-cullin and DCUN1D5-E2 interaction affinities.

Isoforms (3)

UniProt ID	Names	Canonical?
Q92564-1	1	yes
Q92564-2	2
Q92564-3	3

RefSeq proteins (4): NP_001035492, NP_001274684, NP_001274686, NP_055930 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR005176	PONY_dom	Domain
IPR014764	DCN-prot	Family
IPR042460	DCN1-like_PONY	Homologous_superfamily

Pfam: PF03556

UniProt features (27 total): helix 12, strand 3, mutagenesis site 3, turn 2, splice variant 2, chain 1, domain 1, region of interest 1, compositionally biased region 1, cross-link 1

Structure

Experimental structures (PDB)

1 structures.

PDB	Method	Resolution (Å)
5V89	X-RAY DIFFRACTION	1.55

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q92564-F1	81.39	0.65

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (1): 95

Mutagenesis-validated functional residues (3):

Position	Phenotype
250	does not affect localization at nucleus; when associated with r-274 and a-280.
274	does not affect localization at nucleus; when associated with a-250 and a-280.
280	does not affect localization at nucleus; when associated with a-250 and r-274.

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

ID	Pathway
R-HSA-8951664	Neddylation

MSigDB gene sets: 275 (showing top): TGGTGCT_MIR29A_MIR29B_MIR29C, GCM_MAP4K4, MULLIGHAN_NPM1_SIGNATURE_3_UP, RODRIGUES_THYROID_CARCINOMA_POORLY_DIFFERENTIATED_UP, TGCTGCT_MIR15A_MIR16_MIR15B_MIR195_MIR424_MIR497, RORA1_01, AMIT_EGF_RESPONSE_60_HELA, ATACCTC_MIR202, GOBP_PROTEIN_NEDDYLATION, TGACCTY_ERR1_Q2, CACCAGC_MIR138, USF_C, SP1_Q2_01, SCHUHMACHER_MYC_TARGETS_UP, AAACCAC_MIR140

GO Biological Process (2): protein neddylation (GO:0045116), positive regulation of protein neddylation (GO:2000436)

GO Molecular Function (4): ubiquitin conjugating enzyme binding (GO:0031624), ubiquitin-like protein binding (GO:0032182), cullin family protein binding (GO:0097602), protein binding (GO:0005515)

GO Cellular Component (2): ubiquitin ligase complex (GO:0000151), nucleus (GO:0005634)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
Post-translational protein modification	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
protein binding	2
protein modification by small protein conjugation	1
protein neddylation	1
positive regulation of protein modification by small protein conjugation or removal	1
regulation of protein neddylation	1
ubiquitin-like protein conjugating enzyme binding	1
binding	1
intracellular protein-containing complex	1
transferase complex	1
intracellular membrane-bounded organelle	1

Protein interactions and networks

STRING

889 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
DCUN1D4	CWH43	Q9H720	581
DCUN1D4	OCIAD1	Q9NX40	547
DCUN1D4	OCIAD2	Q56VL3	507
DCUN1D4	FBXL13	Q8NEE6	497
DCUN1D4	CUL3	Q13618	477
DCUN1D4	LRRC66	Q68CR7	475
DCUN1D4	SDAD1	Q9NVU7	473
DCUN1D4	COPS2	P61201	468
DCUN1D4	SPATA18	Q8TC71	466
DCUN1D4	DCUN1D1	Q96GG9	450
DCUN1D4	FBXL3	Q9UKT7	442
DCUN1D4	FRYL	O94915	434
DCUN1D4	ARHGAP24	Q8N264	417
DCUN1D4	A0A087WY85	A0A087WY85	412
DCUN1D4	NFXL1	Q6ZNB6	407

IntAct

10 interactions, top by confidence:

A	B	Type	Score
DCUN1D4		psi-mi:“MI:0915”(physical association)	0.490
DDX21	MED19	psi-mi:“MI:2364”(proximity)	0.480
ECE1	DCUN1D4	psi-mi:“MI:0915”(physical association)	0.370
DCUN1D4	ARL4C	psi-mi:“MI:0914”(association)	0.350
DCUN1D4	AFP	psi-mi:“MI:0914”(association)	0.350
NPM1	SBNO1	psi-mi:“MI:2364”(proximity)	0.270
VAV2	DCUN1D4	psi-mi:“MI:0915”(physical association)	0.000

BioGRID (54): CUL1 (Biochemical Activity), CUL2 (Biochemical Activity), CUL3 (Biochemical Activity), CUL4A (Biochemical Activity), CUL4B (Biochemical Activity), CUL5 (Biochemical Activity), DCUN1D4 (Co-fractionation), TOP3A (Co-fractionation), DCUN1D4 (Affinity Capture-MS), CUL1 (Affinity Capture-MS), CUL2 (Affinity Capture-MS), CUL3 (Affinity Capture-MS), CUL5 (Affinity Capture-MS), CAND1 (Affinity Capture-MS), RBX1 (Affinity Capture-MS)

ESM2 similar proteins: A4D161, B1AR13, D3ZV31, E1BBQ2, E1BGQ2, F1N2W9, O42148, O42224, O70524, O95453, P0C7P0, P56726, P97698, Q2T9V7, Q3UGM2, Q497H0, Q56K12, Q5RC51, Q5T6S3, Q5U2M7, Q5XII8, Q5ZIA0, Q60769, Q66J85, Q6DDJ3, Q6DJB3, Q6ZN54, Q7T0P6, Q7Z2E3, Q8BKW4, Q8BYN3, Q8CCA0, Q8IWR0, Q8K214, Q8K2I9, Q8NE86, Q8NFZ0, Q8TDW4, Q92564, Q96GD3

Diamond homologs: A4IHK8, Q1RMX9, Q4V8B2, Q54GP1, Q5ADL9, Q5E9V1, Q5PPL2, Q5R9G1, Q5RDF9, Q5RHX6, Q5ZKU1, Q60YT5, Q6C0B6, Q6DFA1, Q6PH85, Q86JM4, Q8BZJ7, Q8CCA0, Q8IWE4, Q8K0V2, Q8T8S1, Q92564, Q96GG9, Q9BTE7, Q9CXV9, Q9QZ73, Q9U3C8, Q9VUQ8, Q9VWB1, Q5AWS1, Q8WZK4, Q9MBG8, Q4PF67, Q12395, Q750Y3, P0CN06, P0CN07, Q6FJR2

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

38 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	23
Likely benign	1
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

2547 predictions. Top by Δscore:

Variant	Effect	Δscore
4:51843264:GTCA:G	donor_gain	1.0000
4:51843268:G:GG	donor_gain	1.0000
4:51863434:A:AG	acceptor_gain	1.0000
4:51863435:A:G	acceptor_gain	1.0000
4:51863436:G:GA	acceptor_gain	1.0000
4:51863436:GA:G	acceptor_gain	1.0000
4:51863436:GAT:G	acceptor_gain	1.0000
4:51863436:GATT:G	acceptor_gain	1.0000
4:51863436:GATTT:G	acceptor_gain	1.0000
4:51863503:AGCTG:A	donor_gain	1.0000
4:51863504:GCTG:G	donor_gain	1.0000
4:51863504:GCTGG:G	donor_gain	1.0000
4:51863505:CTG:C	donor_gain	1.0000
4:51863505:CTGG:C	donor_loss	1.0000
4:51863507:GGT:G	donor_loss	1.0000
4:51863508:G:GG	donor_gain	1.0000
4:51863508:G:T	donor_loss	1.0000
4:51863509:T:G	donor_loss	1.0000
4:51874270:GGAA:G	acceptor_gain	1.0000
4:51874382:A:G	donor_gain	1.0000
4:51877754:A:AG	acceptor_gain	1.0000
4:51877755:A:G	acceptor_gain	1.0000
4:51877761:A:AG	acceptor_gain	1.0000
4:51877761:AGCAT:A	acceptor_gain	1.0000
4:51877762:G:GA	acceptor_gain	1.0000
4:51877762:GC:G	acceptor_gain	1.0000
4:51877762:GCA:G	acceptor_gain	1.0000
4:51877762:GCAT:G	acceptor_gain	1.0000
4:51877762:GCATG:G	acceptor_gain	1.0000
4:51877851:GCAG:G	donor_gain	1.0000

AlphaMissense

1953 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
4:51877829:C:G	C106W	1.000
4:51877836:T:A	W109R	1.000
4:51877836:T:C	W109R	1.000
4:51886607:T:C	F128S	1.000
4:51886609:T:C	C129R	1.000
4:51886610:G:A	C129Y	1.000
4:51886611:T:G	C129W	1.000
4:51886631:C:A	P136Q	1.000
4:51891770:T:A	L142H	1.000
4:51891770:T:C	L142P	1.000
4:51891779:C:A	A145D	1.000
4:51891794:C:A	A150E	1.000
4:51891805:G:C	G154R	1.000
4:51891806:G:A	G154D	1.000
4:51891812:T:C	F156S	1.000
4:51891826:T:A	W161R	1.000
4:51891826:T:C	W161R	1.000
4:51891827:G:C	W161S	1.000
4:51891828:G:C	W161C	1.000
4:51891828:G:T	W161C	1.000
4:51891835:G:A	G164R	1.000
4:51891835:G:C	G164R	1.000
4:51891836:G:A	G164E	1.000
4:51899290:T:C	L176P	1.000
4:51899352:T:G	Y197D	1.000
4:51899361:G:C	A200P	1.000
4:51899362:C:A	A200E	1.000
4:51899364:T:C	F201L	1.000
4:51899366:T:A	F201L	1.000
4:51899366:T:G	F201L	1.000

dbSNP variants (sampled 300 via entrez): RS1000039344 (4:51839338 G>A,T), RS1000043555 (4:51879269 T>G), RS1000111279 (4:51914646 G>A,T), RS1000149939 (4:51884917 G>A,T), RS1000163884 (4:51915016 G>A), RS1000214392 (4:51833139 T>C), RS1000227149 (4:51898585 A>G), RS1000258977 (4:51902584 C>G,T), RS1000372477 (4:51874562 C>T), RS1000379244 (4:51851133 G>T), RS1000500826 (4:51897486 C>G,T), RS1000508924 (4:51884628 C>T), RS1000517338 (4:51901617 C>G,T), RS1000569997 (4:51901892 G>A,T), RS1000589095 (4:51857463 C>G,T)

Disease associations

OMIM: gene MIM:612977 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

4 associations (top):

Study	Trait	p-value
GCST006088_36	Familial squamous cell lung carcinoma	4.000000e-07
GCST008058_1	Estimated glomerular filtration rate	4.000000e-10
GCST008059_248	Estimated glomerular filtration rate	2.000000e-10
GCST008163_485	Height	1.000000e-06

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0006953	family history of lung cancer

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL4295914 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

2 potent at pChembl≥5 of 3 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
6.09	Ki	807	nM	CHEMBL4592844
6.09	Ki	810	nM	CHEMBL4592844

PubChem BioAssay actives

2 with measured affinity, of 6 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-(4-methylphenyl)-2-prop-2-ynylsulfanyl-6-(1,3-thiazol-2-ylsulfanyl)pyrimidine-5-carbonitrile	1599586: Binding affinity to N-terminal GST tagged recombinant DCN4 (unknown origin) (102 to 292 residues) expressed in Escherichia coli BL21(DE3) assessed as reduction in DCN1/FAM-labelled N-terminal acetylated UBE2M (1 to 21 residues) protein-protein interaction after 30 mins by HTRF assay	ki	0.8070	uM

CTD chemical–gene interactions

41 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Valproic Acid	affects cotreatment, decreases expression, affects expression, increases methylation	7
trichostatin A	affects cotreatment, decreases expression	3
potassium chromate(VI)	affects cotreatment, decreases expression	2
Phenylmercuric Acetate	affects cotreatment, decreases expression	2
aristolochic acid I	decreases expression	1
FR900359	decreases phosphorylation	1
dicrotophos	decreases expression	1
methylmercuric chloride	decreases expression	1
triphenyl phosphate	affects expression	1
salinomycin	decreases expression	1
beta-lapachone	decreases expression	1
sodium arsenite	decreases expression	1
epigallocatechin gallate	decreases expression, affects cotreatment	1
chromium hexavalent ion	decreases expression	1
CGP 52608	affects binding, increases reaction	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, decreases expression	1
dorsomorphin	affects cotreatment, decreases expression	1
bisphenol S	affects cotreatment, increases expression	1
jinfukang	decreases expression	1
picoxystrobin	increases expression	1
PCI 5002	affects cotreatment, increases expression	1
Temozolomide	decreases expression	1
Zoledronic Acid	increases expression	1
Vorinostat	increases expression	1
Panobinostat	affects cotreatment, decreases expression	1
Acetaminophen	decreases expression	1
Dexamethasone	increases expression, affects cotreatment	1
Indomethacin	affects cotreatment, increases expression	1
Methyl Methanesulfonate	decreases expression	1
Phthalic Acids	increases methylation	1

ChEMBL screening assays

5 unique, capped per target: 5 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL4222551	Binding	Inhibition of human DCN4-mediated cullin neddylation assessed as reduction in transfer of fluorescein-5-maleimide-tagged NEDD8 from N-terminally acetylated human UBE2M to CUL2-CTD at 50 uM measured for 1 min by pulse-chase assay	Piperidinyl Ureas Chemically Control Defective in Cullin Neddylation 1 (DCN1)-Mediated Cullin Neddylation. — J Med Chem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.