DDX59

Summary

DDX59 (DEAD-box helicase 59, HGNC:25360) is a protein-coding gene on chromosome 1q32.1, encoding Probable ATP-dependent RNA helicase DDX59 (Q5T1V6). It is a common-essential gene (DepMap: required in 92.6% of cancer cell lines).

Predicted to enable RNA helicase activity and mRNA binding activity. Predicted to be located in cytoplasm; membrane; and nucleus. Implicated in orofaciodigital syndrome V.

Source: NCBI Gene 83479 — RefSeq curated summary.

At a glance

• Gene–disease (curated): orofaciodigital syndrome V (Strong, GenCC)
• GWAS associations: 4
• Clinical variants (ClinVar): 181 total — 5 pathogenic, 3 likely-pathogenic
• Phenotypes (HPO): 60
• Druggable target: yes
• Cancer dependency (DepMap): dependent in 92.6% of screened cell lines (common-essential)
• MANE Select transcript: NM_001031725

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:25360
Approved symbol	DDX59
Name	DEAD-box helicase 59
Location	1q32.1
Locus type	gene with protein product
Status	Approved
Aliases	DKFZP564B1023, ZNHIT5
Ensembl gene	ENSG00000118197
Ensembl biotype	protein_coding
OMIM	615464
Entrez	83479

Gene structure

Transcript identifiers

Ensembl transcripts: 26 — 26 protein_coding

ENST00000331314, ENST00000413408, ENST00000429498, ENST00000433235, ENST00000436897, ENST00000447706, ENST00000452560, ENST00000453944, ENST00000886515, ENST00000886516, ENST00000886517, ENST00000886518, ENST00000936158, ENST00000936159, ENST00000936160, ENST00000936161, ENST00000936162, ENST00000958770, ENST00000958771, ENST00000958772, ENST00000958773, ENST00000958774, ENST00000958775, ENST00000958776, ENST00000958777, ENST00000958778

RefSeq mRNA: 9 — MANE Select: NM_001031725 NM_001031725, NM_001320181, NM_001320182, NM_001349799, NM_001349800, NM_001349801, NM_001349802, NM_001349803, NM_001349804

CCDS: CCDS30964

Canonical transcript exons

ENST00000331314 — 8 exons

Exon	Start	End
ENSE00000796420	200648439	200648567
ENSE00000796421	200649074	200649226
ENSE00001827363	200669767	200669907
ENSE00001909196	200644037	200644517
ENSE00002284446	200659027	200659116
ENSE00002349402	200663919	200664086
ENSE00002707839	200665937	200666751
ENSE00003489109	200650425	200650676

Expression profiles

Bgee: expression breadth ubiquitous, 256 present calls, max score 99.36.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 9.6743 / max 119.1715, expressed in 1769 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
16590	9.4673	1763
16589	0.2070	78

Top tissues by expression

256 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
buccal mucosa cell	CL:0002336	99.36	gold quality
pancreatic ductal cell	CL:0002079	99.24	gold quality
tendon of biceps brachii	UBERON:0008188	98.64	gold quality
oocyte	CL:0000023	98.07	gold quality
secondary oocyte	CL:0000655	98.02	gold quality
cardia of stomach	UBERON:0001162	95.94	gold quality
endothelial cell	CL:0000115	95.85	silver quality
mucosa of paranasal sinus	UBERON:0005030	95.82	gold quality
renal medulla	UBERON:0000362	95.31	gold quality
kidney epithelium	UBERON:0004819	95.31	silver quality
nipple	UBERON:0002030	95.08	gold quality
ventral tegmental area	UBERON:0002691	95.08	gold quality
lateral globus pallidus	UBERON:0002476	94.98	silver quality
superficial temporal artery	UBERON:0001614	94.71	gold quality
subthalamic nucleus	UBERON:0001906	94.62	gold quality
pylorus	UBERON:0001166	94.60	gold quality
globus pallidus	UBERON:0001875	94.45	gold quality
superior surface of tongue	UBERON:0007371	94.45	gold quality
tendon	UBERON:0000043	94.38	gold quality
dorsal plus ventral thalamus	UBERON:0001897	94.38	gold quality
upper arm skin	UBERON:0004263	94.38	gold quality
inferior vagus X ganglion	UBERON:0005363	94.29	gold quality
medial globus pallidus	UBERON:0002477	94.26	gold quality
substantia nigra pars reticulata	UBERON:0001966	94.22	gold quality
trachea	UBERON:0003126	94.16	gold quality
medulla oblongata	UBERON:0001896	94.15	gold quality
vena cava	UBERON:0004087	93.89	gold quality
superior vestibular nucleus	UBERON:0007227	93.81	gold quality
lateral nuclear group of thalamus	UBERON:0002736	93.78	gold quality
pericardium	UBERON:0002407	93.75	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	5.00
E-GEOD-110499	no	494.38

Regulation

Is transcription factor: no

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 92.6% of screened cell lines, common-essential.

Literature-anchored findings (GeneRIF, showing 5)

• These data strongly implicate DDX59 RNA helicase family member in the pathogenesis of orofaciodigital syndrome. (PMID:23972372)
• The novel DDX59 mutation is associated with oral-facial-digital syndrome. (PMID:28711741)
• Analysis of human brain gene expression provides evidence that DDX59 is enriched in oligodendrocytes and might act within pathways of leukoencephalopathies-associated genes. (PMID:29127725)
• Study presents evidence that DDX59 protein can be upregulated by MEK/ERK/MAP kinase pathway downstream of EGFR and Ras signaling. Increased expression of DDX59 mediates the tumorigenecity of EGFR and Ras in lung cancer. (PMID:29133145)
• MicroRNA-628-5p inhibits cell proliferation in glioma by targeting DDX59. (PMID:31111544)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	ddx59	ENSDARG00000022177
mus_musculus	Ddx59	ENSMUSG00000026404
rattus_norvegicus	Ddx59	ENSRNOG00000042451

Paralogs (38): DDX20 (ENSG00000064703), DDX3Y (ENSG00000067048), DDX1 (ENSG00000079785), DDX43 (ENSG00000080007), DDX18 (ENSG00000088205), DDX24 (ENSG00000089737), DDX17 (ENSG00000100201), DDX49 (ENSG00000105671), DDX50 (ENSG00000107625), DDX5 (ENSG00000108654), DDX25 (ENSG00000109832), DDX6 (ENSG00000110367), DDX55 (ENSG00000111364), DDX54 (ENSG00000123064), DDX39A (ENSG00000123136), DDX27 (ENSG00000124228), DDX31 (ENSG00000125485), DDX56 (ENSG00000136271), EIF4A3 (ENSG00000141543), DDX46 (ENSG00000145833), DDX4 (ENSG00000152670), EIF4A2 (ENSG00000156976), DDX19B (ENSG00000157349), EIF4A1 (ENSG00000161960), DDX21 (ENSG00000165732), DDX19A (ENSG00000168872), TDRD12 (ENSG00000173809), DDX23 (ENSG00000174243), DDX10 (ENSG00000178105), DDX28 (ENSG00000182810), DDX41 (ENSG00000183258), DDX53 (ENSG00000184735), DDX51 (ENSG00000185163), DDX42 (ENSG00000198231), DDX39B (ENSG00000198563), DDX47 (ENSG00000213782), DDX3X (ENSG00000215301), DDX52 (ENSG00000278053)

Protein

Protein identifiers

Probable ATP-dependent RNA helicase DDX59 — Q5T1V6 (reviewed: Q5T1V6)

Alternative names: DEAD box protein 59, Zinc finger HIT domain-containing protein 5

All UniProt accessions (7): B7ZBU3, B7ZBU4, Q5T1V6, H0Y6Y1, H0Y7Z8, Q5T1V5, Q5T1W1

UniProt curated annotations — full annotation on UniProt →

Subunit / interactions. Interacts (via HIT-type zinc finger) with the RUVBL1/RUVBL2 complex in the presence of ADP.

Subcellular location. Cytoplasm. Nucleus.

Tissue specificity. Expressed in fibroblasts (at protein level).

Disease relevance. Orofaciodigital syndrome 5 (OFD5) [MIM:174300] A form of orofaciodigital syndrome, a group of heterogeneous disorders characterized by malformations of the oral cavity, face and digits, and associated phenotypic abnormalities that lead to the delineation of various subtypes. OFD5 patients show the core features of cleft palate, lobulated tongue, and polydactyly. Additional features include frontal bossing and intellectual disability. The disease is caused by variants affecting the gene represented in this entry.

Domain organisation. The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Similarity. Belongs to the DEAD box helicase family. DDX59 subfamily.

Isoforms (2)

UniProt ID	Names	Canonical?
Q5T1V6-1	1	yes
Q5T1V6-2	2

RefSeq proteins (9): NP_001026895, NP_001307110, NP_001307111, NP_001336728, NP_001336729, NP_001336730, NP_001336731, NP_001336732, NP_001336733 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001650	Helicase_C-like	Domain
IPR007529	Znf_HIT	Domain
IPR011545	DEAD/DEAH_box_helicase_dom	Domain
IPR014001	Helicase_ATP-bd	Domain
IPR014014	RNA_helicase_DEAD_Q_motif	Domain
IPR027417	P-loop_NTPase	Homologous_superfamily
IPR050079	DEAD_box_RNA_helicase	Family

Pfam: PF00270, PF00271, PF04438

Catalyzed reactions (Rhea), 1 shown:

• ATP + H2O = ADP + phosphate + H(+) (RHEA:13065)

UniProt features (26 total): sequence variant 5, modified residue 4, strand 4, domain 2, region of interest 2, short sequence motif 2, chain 1, cross-link 1, splice variant 1, helix 1, zinc finger region 1, compositionally biased region 1, binding site 1

Structure

Experimental structures (PDB)

1 structures.

PDB	Method	Resolution (Å)
2YQP	SOLUTION NMR

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q5T1V6-F1	77.71	0.46

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (1): 247–254

Post-translational modifications (5): 64, 76, 156, 160, 26

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 243 (showing top): PYEON_CANCER_HEAD_AND_NECK_VS_CERVICAL_UP, IVANOVA_HEMATOPOIESIS_EARLY_PROGENITOR, KRIGE_RESPONSE_TO_TOSEDOSTAT_24HR_UP, GOMF_MRNA_BINDING, GOMF_HYDROLASE_ACTIVITY_ACTING_ON_ACID_ANHYDRIDES, LIU_COMMON_CANCER_GENES, GSE13762_CTRL_VS_125_VITAMIND_DAY5_DC_DN, GOMF_ATP_HYDROLYSIS_ACTIVITY, GOMF_ADENYL_NUCLEOTIDE_BINDING, GOMF_ISOMERASE_ACTIVITY, BRUINS_UVC_RESPONSE_LATE, ZWANG_CLASS_1_TRANSIENTLY_INDUCED_BY_EGF, GOMF_CATALYTIC_ACTIVITY_ACTING_ON_RNA, GOMF_ATP_DEPENDENT_ACTIVITY_ACTING_ON_RNA, BARX1_TARGET_GENES

GO Biological Process (0):

GO Molecular Function (11): RNA helicase activity (GO:0003724), mRNA binding (GO:0003729), ATP binding (GO:0005524), zinc ion binding (GO:0008270), ATP hydrolysis activity (GO:0016887), nucleotide binding (GO:0000166), nucleic acid binding (GO:0003676), RNA binding (GO:0003723), helicase activity (GO:0004386), hydrolase activity (GO:0016787), metal ion binding (GO:0046872)

GO Cellular Component (3): nucleus (GO:0005634), cytoplasm (GO:0005737), membrane (GO:0016020)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
ATP-dependent activity	2
cellular anatomical structure	2
helicase activity	1
ATP-dependent activity, acting on RNA	1
catalytic activity, acting on RNA	1
RNA binding	1
adenyl ribonucleotide binding	1
purine ribonucleoside triphosphate binding	1
transition metal ion binding	1
ribonucleoside triphosphate phosphatase activity	1
nucleoside phosphate binding	1
heterocyclic compound binding	1
binding	1
nucleic acid binding	1
nucleic acid conformation isomerase activity	1
catalytic activity, acting on a nucleic acid	1
catalytic activity	1
cation binding	1
intracellular membrane-bounded organelle	1
intracellular anatomical structure	1

Protein interactions and networks

STRING

2571 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
DDX59	TBC1D32	Q96NH3	571
DDX59	TCTN3	Q6NUS6	570
DDX59	CPLANE1	Q9H799	543
DDX59	DHX30	Q7L2E3	534
DDX59	OFD1	O75665	497
DDX59	TMEM216	Q9P0N5	477
DDX59	TMEM231	Q9H6L2	473
DDX59	INO80E	Q8NBZ0	471
DDX59	CAMSAP2	Q08AD1	460
DDX59	DHX34	Q14147	459
DDX59	SCLT1	Q96NL6	456
DDX59	MRPL20	Q9BYC9	454
DDX59	C5orf52	A6NGY3	448
DDX59	C2CD3	Q4AC94	448
DDX59	DHX16	O60231	426

IntAct

8 interactions, top by confidence:

A	B	Type	Score
DDX59	ARG2	psi-mi:“MI:0915”(physical association)	0.400
FKBP5	IFT56	psi-mi:“MI:0914”(association)	0.350
LMNB2	SPOP	psi-mi:“MI:0914”(association)	0.350
HSD17B14	E2F6	psi-mi:“MI:0914”(association)	0.350
MTPN	PLCG1	psi-mi:“MI:0914”(association)	0.350
H2BC10	SMCHD1	psi-mi:“MI:2364”(proximity)	0.270

BioGRID (27): DDX59 (Affinity Capture-RNA), DDX59 (Affinity Capture-RNA), DDX59 (Proximity Label-MS), DDX59 (Affinity Capture-MS), HSP90AA1 (Affinity Capture-MS), HSP90AB1 (Affinity Capture-MS), HSPA8 (Affinity Capture-MS), STIP1 (Affinity Capture-MS), DDX59 (Affinity Capture-MS), DDX59 (Negative Genetic), DDX59 (Affinity Capture-RNA), DDX59 (Negative Genetic), DDX59 (Proximity Label-MS), DDX59 (Affinity Capture-MS), DDX59 (Affinity Capture-MS)

ESM2 similar proteins: A2VD92, A5D7C1, A5DIX5, A5E1N2, A6ZU15, O16102, O74393, P23394, P45818, P54823, Q07886, Q09775, Q0DBS1, Q0IHV9, Q0IIK5, Q10202, Q19614, Q4R7L5, Q55CP6, Q5NVJ8, Q5T1V6, Q5XH91, Q641Y8, Q6AZV7, Q6C024, Q6CDS6, Q6CKI1, Q6FM43, Q7FGZ2, Q84T03, Q86TM3, Q8GXD6, Q90WU3, Q91VN6, Q91VR5, Q92499, Q9C551, Q9DBN9, Q9DF35, Q9FLB0

Diamond homologs: A1CH78, A2QI25, A3LQ01, A5DQS0, A5DZE6, A5F3R4, A6SEH9, A6ZP47, A7EJY3, A7TKR8, A8WK63, C3LQR1, C6DHF5, C8V8H4, D4ACP5, O09053, O13370, O13792, O13983, O18017, O34748, O88700, O93530, O94761, O94762, P06634, P0CQ88, P0CQ89, P15043, P35187, P40724, P46063, P50729, P54132, P71359, P73421, Q09811, Q0CLJ6, Q0ILZ4, Q0JM17

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

181 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	5
Likely pathogenic	3
Uncertain significance	90
Likely benign	42
Benign	21

Top pathogenic / likely-pathogenic (8)

Variant ID	HGVS	Classification
1332918	NM_001031725.6(DDX59):c.1859G>T (p.Ter620Leu)	Pathogenic
2008692	NM_001031725.6(DDX59):c.454C>T (p.Gln152Ter)	Pathogenic
4689396	NC_000001.10:g.(?200613164)(200613646_200617566)del	Pathogenic
591043	NM_001031725.6(DDX59):c.1136del (p.Gln379fs)	Pathogenic
801603	NM_001031725.6(DDX59):c.185del (p.Phe62fs)	Pathogenic
1299500	NM_001031725.6(DDX59):c.1597-6T>G	Likely pathogenic
2769652	NM_001031725.6(DDX59):c.804+1G>A	Likely pathogenic
800916	NM_001031725.6(DDX59):c.1648AAT[2] (p.Asn552del)	Likely pathogenic

SpliceAI

1345 predictions. Top by Δscore:

Variant	Effect	Δscore
1:200649088:T:A	donor_gain	1.0000
1:200650423:A:AC	donor_gain	1.0000
1:200650424:C:CC	donor_gain	1.0000
1:200659025:A:AC	donor_gain	1.0000
1:200659026:C:CC	donor_gain	1.0000
1:200659035:C:CT	donor_gain	1.0000
1:200659036:T:TT	donor_gain	1.0000
1:200659113:TAAC:T	acceptor_gain	1.0000
1:200659114:AACC:A	acceptor_loss	1.0000
1:200659115:ACC:A	acceptor_loss	1.0000
1:200659116:CCTAA:C	acceptor_loss	1.0000
1:200659117:C:CC	acceptor_gain	1.0000
1:200659118:T:C	acceptor_loss	1.0000
1:200663913:GCTTA:G	donor_loss	1.0000
1:200663914:CTTA:C	donor_loss	1.0000
1:200663915:TTAC:T	donor_loss	1.0000
1:200663916:TACCT:T	donor_loss	1.0000
1:200663917:A:C	donor_loss	1.0000
1:200664083:TGCT:T	acceptor_gain	1.0000
1:200664087:C:CC	acceptor_gain	1.0000
1:200664097:G:C	acceptor_gain	1.0000
1:200665932:CTTA:C	donor_loss	1.0000
1:200665934:TAC:T	donor_loss	1.0000
1:200665935:A:AC	donor_gain	1.0000
1:200665935:A:AT	donor_loss	1.0000
1:200665936:C:CC	donor_gain	1.0000
1:200665936:CCT:C	donor_gain	1.0000
1:200666748:TATT:T	acceptor_gain	1.0000
1:200648573:C:CT	acceptor_gain	0.9900
1:200648574:A:T	acceptor_gain	0.9900

AlphaMissense

4078 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
1:200648532:G:C	S501R	0.999
1:200648532:G:T	S501R	0.999
1:200648534:T:G	S501R	0.999
1:200644481:C:G	A545P	0.998
1:200648482:A:T	V518D	0.998
1:200649195:A:T	V449E	0.998
1:200666357:A:C	S128R	0.998
1:200666357:A:T	S128R	0.998
1:200666359:T:G	S128R	0.998
1:200666419:A:G	C108R	0.998
1:200644390:A:G	L575S	0.997
1:200644480:G:T	A545E	0.997
1:200644509:T:A	R535S	0.997
1:200644509:T:G	R535S	0.997
1:200649154:C:G	A463P	0.997
1:200649165:A:G	L459P	0.997
1:200650452:T:A	K429N	0.997
1:200650452:T:G	K429N	0.997
1:200650472:A:G	W423R	0.997
1:200650472:A:T	W423R	0.997
1:200659106:G:T	A328E	0.997
1:200666350:A:G	C131R	0.997
1:200666362:A:G	C127R	0.997
1:200644510:C:G	R535T	0.996
1:200648536:A:T	V500E	0.996
1:200649162:A:G	L460S	0.996
1:200649172:C:G	A457P	0.996
1:200650650:A:C	F363L	0.996
1:200650650:A:T	F363L	0.996
1:200650652:A:G	F363L	0.996

dbSNP variants (sampled 300 via entrez): RS1000012685 (1:200664889 G>A), RS1000098125 (1:200669701 G>C), RS1000319232 (1:200646530 A>G), RS1000576953 (1:200654420 GA>G,GAA), RS1000655336 (1:200645207 A>G), RS1000690704 (1:200648275 C>T), RS1000833960 (1:200651818 C>T), RS1000979853 (1:200652236 T>C), RS1001099290 (1:200657674 G>A), RS1001228787 (1:200648650 A>G,T), RS1001291630 (1:200669295 C>A,G), RS1001484035 (1:200667858 T>A,C,G), RS1001534133 (1:200646187 A>G), RS1001616708 (1:200652827 T>C), RS1001668982 (1:200653064 G>A)

Disease associations

OMIM: gene MIM:615464 | disease phenotypes: MIM:174300

GenCC curated gene-disease

Disease	Classification	Inheritance
orofaciodigital syndrome V	Strong	Autosomal recessive

Mondo (1): orofaciodigital syndrome V (MONDO:0008267)

Orphanet (1): Orofaciodigital syndrome type 5 (Orphanet:2919)

HPO phenotypes

60 total (30 of 60 shown, HPO-id order):

HPO	Term
HP:0000007	Autosomal recessive inheritance
HP:0000085	Horseshoe kidney
HP:0000161	Median cleft upper lip
HP:0000175	Cleft palate
HP:0000180	Lobulated tongue
HP:0000185	Cleft soft palate
HP:0000190	Abnormal oral frenulum morphology
HP:0000191	Accessory oral frenulum
HP:0000193	Bifid uvula
HP:0000218	High palate
HP:0000219	Thin upper lip vermilion
HP:0000252	Microcephaly
HP:0000288	Abnormality of the philtrum
HP:0000303	Mandibular prognathia
HP:0000316	Hypertelorism
HP:0000324	Facial asymmetry
HP:0000365	Hearing impairment
HP:0000369	Low-set ears
HP:0000494	Downslanted palpebral fissures
HP:0000574	Thick eyebrow
HP:0000588	Optic disc coloboma
HP:0000668	Hypodontia
HP:0000689	Dental malocclusion
HP:0001162	Postaxial hand polydactyly
HP:0001212	Prominent fingertip pads
HP:0001249	Intellectual disability
HP:0001263	Global developmental delay
HP:0001274	Agenesis of corpus callosum
HP:0001629	Ventricular septal defect
HP:0001636	Tetralogy of Fallot

GWAS associations

4 associations (top):

Study	Trait	p-value
GCST005195_41	Coronary artery disease	2.000000e-08
GCST005196_191	Coronary artery disease	4.000000e-09
GCST010002_373	Refractive error	1.000000e-52
GCST012490_338	Femur bone mineral density x serum urate levels interaction	2.000000e-09

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0004531	urate measurement

MeSH disease descriptors (1)

Descriptor	Name	Tree numbers
C557819	Orofaciodigital syndrome 5 (supp.)

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL5465317 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

30 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Valproic Acid	affects expression, decreases expression	6
Cyclosporine	increases expression	3
aristolochic acid I	decreases expression	1
FR900359	increases phosphorylation	1
dicrotophos	decreases expression	1
triphenyl phosphate	affects expression	1
titanium dioxide	increases methylation	1
dimethylselenide	increases expression, increases oxidation	1
beta-lapachone	increases expression	1
tris(1,3-dichloro-2-propyl)phosphate	increases expression	1
sodium arsenite	increases stability	1
coumarin	affects phosphorylation	1
beta-methylcholine	affects expression	1
avobenzone	increases expression	1
K 7174	increases expression	1
Benzo(a)pyrene	increases methylation	1
Caffeine	affects phosphorylation	1
Cisplatin	increases expression	1
Dimethyl Sulfoxide	increases expression	1
Doxorubicin	decreases expression	1
Enzyme Inhibitors	decreases activity, increases O-linked glycosylation	1
Formaldehyde	increases expression	1
Methyl Methanesulfonate	increases expression	1
Ozone	increases expression, increases oxidation	1
Quercetin	decreases phosphorylation	1
Silicon Dioxide	decreases expression, increases methylation	1
Smoke	decreases expression	1
Tretinoin	decreases expression	1
Aflatoxin B1	decreases methylation	1
Hydroxyl Radical	increases expression, increases oxidation	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5391273	Binding	Inhibition of C-terminal 6xHis-tagged human recombinant DDX59 transformed in Escherichia coli BL21(DE3)pLysS cells	Development of small molecule inhibitors targeting RNA helicase DHX33 as anti-cancer agents. — Bioorg Med Chem Lett

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.