DNAJC22

Summary

DNAJC22 (DnaJ heat shock protein family (Hsp40) member C22, HGNC:25802) is a protein-coding gene on chromosome 12q13.12, encoding DnaJ homolog subfamily C member 22 (Q8N4W6). May function as a co-chaperone.

Predicted to be active in membrane.

Source: NCBI Gene 79962 — RefSeq curated summary.

At a glance

• GWAS associations: 6
• Clinical variants (ClinVar): 66 total
• MANE Select transcript: NM_001304944

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:25802
Approved symbol	DNAJC22
Name	DnaJ heat shock protein family (Hsp40) member C22
Location	12q13.12
Locus type	gene with protein product
Status	Approved
Aliases	wus, FLJ13236
Ensembl gene	ENSG00000178401
Ensembl biotype	protein_coding
Entrez	79962

Gene structure

Transcript identifiers

Ensembl transcripts: 8 — 5 protein_coding, 1 retained_intron, 1 protein_coding_CDS_not_defined, 1 nonsense_mediated_decay

ENST00000395069, ENST00000549441, ENST00000551153, ENST00000552651, ENST00000647553, ENST00000851339, ENST00000851340, ENST00000851341

RefSeq mRNA: 2 — MANE Select: NM_001304944 NM_001304944, NM_024902

CCDS: CCDS8785

Canonical transcript exons

ENST00000549441 — 4 exons

Exon	Start	End
ENSE00001264564	49348766	49349712
ENSE00001520484	49351317	49353690
ENSE00002330642	49347644	49348105
ENSE00002367086	49346888	49347551

Expression profiles

Bgee: expression breadth ubiquitous, 238 present calls, max score 94.90.

FANTOM5 (CAGE): breadth broad, TPM avg 1.9728 / max 39.2197, expressed in 673 samples.

FANTOM5 promoters (4 alternative TSS)

Promoter ID	TPM avg	Samples expressed
125329	1.5095	605
125328	0.1999	121
125327	0.1965	113
125330	0.0669	27

Top tissues by expression

280 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
right lobe of liver	UBERON:0001114	94.90	gold quality
liver	UBERON:0002107	91.00	gold quality
heart right ventricle	UBERON:0002080	90.69	silver quality
jejunal mucosa	UBERON:0000399	89.82	gold quality
body of tongue	UBERON:0011876	89.72	silver quality
lateral nuclear group of thalamus	UBERON:0002736	89.56	silver quality
skeletal muscle tissue of rectus abdominis	UBERON:0004511	89.40	silver quality
vena cava	UBERON:0004087	89.28	silver quality
pons	UBERON:0000988	88.95	gold quality
subthalamic nucleus	UBERON:0001906	88.69	gold quality
cardia of stomach	UBERON:0001162	88.66	silver quality
duodenum	UBERON:0002114	88.61	gold quality
pylorus	UBERON:0001166	88.14	gold quality
inferior vagus X ganglion	UBERON:0005363	88.00	silver quality
jejunum	UBERON:0002115	87.97	gold quality
ventral tegmental area	UBERON:0002691	87.74	silver quality
lateral globus pallidus	UBERON:0002476	87.33	gold quality
pharyngeal mucosa	UBERON:0000355	87.20	silver quality
pancreatic ductal cell	CL:0002079	86.98	silver quality
mucosa of transverse colon	UBERON:0004991	86.95	gold quality
tongue	UBERON:0001723	86.74	silver quality
pericardium	UBERON:0002407	86.60	silver quality
superior vestibular nucleus	UBERON:0007227	86.49	silver quality
substantia nigra pars reticulata	UBERON:0001966	86.41	gold quality
superior surface of tongue	UBERON:0007371	86.41	silver quality
endometrium epithelium	UBERON:0004811	86.20	gold quality
substantia nigra pars compacta	UBERON:0001965	86.19	silver quality
gall bladder	UBERON:0002110	85.86	gold quality
renal medulla	UBERON:0000362	85.77	gold quality
saphenous vein	UBERON:0007318	85.18	silver quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	4.50

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

83 targeting DNAJC22, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-4425	100.00	67.59	1049
HSA-MIR-3163	100.00	77.23	8605
HSA-MIR-5011-5P	100.00	83.46	5820
HSA-MIR-6798-5P	100.00	65.77	699
HSA-MIR-5692B	100.00	71.32	2622
HSA-MIR-5692C	100.00	71.32	2622
HSA-MIR-150-5P	99.99	66.69	1976
HSA-MIR-4531	99.99	69.70	3181
HSA-MIR-4775	99.98	75.00	6394
HSA-MIR-302C-5P	99.97	72.56	3642
HSA-MIR-3658	99.96	73.87	4379
HSA-MIR-302E	99.96	70.74	2669
HSA-MIR-6778-3P	99.96	67.29	2693
HSA-MIR-3912-5P	99.95	66.11	925
HSA-MIR-374A-5P	99.90	71.34	2923
HSA-MIR-374B-5P	99.90	69.98	2734
HSA-MIR-124-3P	99.89	73.74	3043
HSA-MIR-506-3P	99.89	73.55	3057
HSA-MIR-9902	99.89	69.15	2250
HSA-MIR-7845-5P	99.88	64.88	771
HSA-MIR-576-5P	99.84	70.46	2582
HSA-MIR-6756-5P	99.82	67.97	2466
HSA-MIR-4694-3P	99.79	69.53	2640
HSA-MIR-4713-5P	99.78	67.80	1794
HSA-MIR-3913-5P	99.78	67.26	968
HSA-MIR-2116-3P	99.74	64.32	889
HSA-MIR-6745	99.74	65.33	1321
HSA-MIR-4524A-3P	99.72	66.85	2406
HSA-MIR-6766-5P	99.68	67.70	2325
HSA-MIR-466	99.67	70.85	2863

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	dnajc22	ENSDARG00000037067
mus_musculus	Dnajc22	ENSMUSG00000038009
rattus_norvegicus	Dnajc22	ENSRNOG00000053498
drosophila_melanogaster	wus	FBGN0030805

Protein

Protein identifiers

DnaJ homolog subfamily C member 22 — Q8N4W6 (reviewed: Q8N4W6)

All UniProt accessions (1): Q8N4W6

UniProt curated annotations — full annotation on UniProt →

Function. May function as a co-chaperone.

Subcellular location. Membrane.

RefSeq proteins (2): NP_001291873, NP_079178 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001623	DnaJ_domain	Domain
IPR007829	TM2	Domain
IPR036869	J_dom_sf	Homologous_superfamily

Pfam: PF00226, PF05154

UniProt features (10 total): transmembrane region 7, domain 2, chain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q8N4W6-F1	81.46	0.39

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 122 (showing top): MORF_ITGA2, WANG_CLIM2_TARGETS_UP, BENPORATH_ES_WITH_H3K27ME3, NKX25_02, LUCAS_HNF4A_TARGETS_UP, MORF_RAD51L3, MODULE_205, MORF_CTSB, YY1_02, ROZANOV_MMP14_TARGETS_UP, MORF_IL4, MORF_PRKCA, HNF4_DR1_Q3, MORF_THPO, MORF_ATF2

GO Biological Process (0):

GO Molecular Function (1): protein binding (GO:0005515)

GO Cellular Component (1): membrane (GO:0016020)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
binding	1
cellular anatomical structure	1

Protein interactions and networks

STRING

1671 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
DNAJC22	DNAJC18	Q9H819	559
DNAJC22	DNAJC28	Q9NX36	558
DNAJC22	DNAJC9	Q8WXX5	552
DNAJC22	DNAJC27	Q9NZQ0	545
DNAJC22	DNAJC24	Q6P3W2	540
DNAJC22	DNAJC25	Q9H1X3	511
DNAJC22	LBX1	P52954	510
DNAJC22	DNAJC8	O75937	495
DNAJC22	LMBR1L	Q6UX01	490
DNAJC22	DNAJB13	P59910	472
DNAJC22	DNAJC4	Q9NNZ3	463
DNAJC22	DNAJC13	O75165	448
DNAJC22	DNAJC1	Q96KC8	448
DNAJC22	DNAJC2	Q99543	446
DNAJC22	SEPTIN3	Q9UH03	445

IntAct

6 interactions, top by confidence:

A	B	Type	Score
MEOX2	DNAJC22	psi-mi:“MI:0915”(physical association)	0.560
DNAJC22	UNC13B	psi-mi:“MI:0914”(association)	0.350
LYPD3	NEMP1	psi-mi:“MI:0914”(association)	0.350
DNAJC22	MEOX2	psi-mi:“MI:0915”(physical association)	0.000

BioGRID (39): DNAJC22 (Two-hybrid), ENSA (Affinity Capture-MS), RPN1 (Affinity Capture-MS), SDHA (Affinity Capture-MS), RANBP3 (Affinity Capture-MS), CMAS (Affinity Capture-MS), LARP7 (Affinity Capture-MS), TRIM13 (Proximity Label-MS), CKAP4 (Proximity Label-MS), ADCY9 (Proximity Label-MS), TMEM199 (Proximity Label-MS), ECH1 (Proximity Label-MS), EMD (Proximity Label-MS), STT3B (Proximity Label-MS), EMC1 (Proximity Label-MS)

ESM2 similar proteins: A9ULE9, B0K025, B1X3F0, E2RKN8, F1LXS7, O06752, O24301, O28116, O29246, O30092, P18033, P27709, P29574, P31924, P49036, P55522, P61165, P61166, P64445, P64446, P64447, P86218, Q01390, Q17QW0, Q28IL7, Q2HJ69, Q2KID7, Q32P84, Q3AKY6, Q3AYG7, Q43009, Q57938, Q5M9B7, Q5PR00, Q5RBD7, Q5ZJR3, Q5ZLR7, Q6GLZ9, Q6GNY6, Q6PBS6

Diamond homologs: A0A0P0VG31, A5IIT4, A9ULE9, B1LCI2, B7IFE0, B9KAB9, Q17QW0, Q54YM7, Q5PR00, Q5RBD7, Q8CHS2, Q8N4W6, Q97BG9, Q9GPR3, Q9HJ83, Q9WZV3, A0Q1R3, A4XKA5, A5EYE5, A6LJ63, A9VHU0, B0SHT0, B0SRF0, B2TLZ8, B2V2I6, B7HCT9, B7HPL2, B7IYG6, B7JN38, B9E6X0, B9IY80, B9MJZ0, C1ESK7, C3L5R6, C3P8L9, O35723, O54946, O75190, O94625, P30725

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

66 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	56
Likely benign	3
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

749 predictions. Top by Δscore:

Variant	Effect	Δscore
12:49347198:G:GT	donor_gain	1.0000
12:49347199:A:T	donor_gain	1.0000
12:49349713:GTAA:G	donor_loss	1.0000
12:49351312:A:AG	acceptor_gain	1.0000
12:49351312:ATAAG:A	acceptor_gain	1.0000
12:49351313:T:G	acceptor_gain	1.0000
12:49351314:A:AG	acceptor_gain	1.0000
12:49351314:AAG:A	acceptor_gain	1.0000
12:49351315:A:G	acceptor_gain	1.0000
12:49347549:GAGG:G	donor_loss	0.9900
12:49347550:AGGT:A	donor_loss	0.9900
12:49347552:GTGAG:G	donor_loss	0.9900
12:49347553:T:A	donor_loss	0.9900
12:49348872:G:GT	donor_gain	0.9900
12:49349593:C:A	acceptor_gain	0.9900
12:49349714:T:G	donor_loss	0.9900
12:49351313:TAA:T	acceptor_loss	0.9900
12:49351315:A:AC	acceptor_loss	0.9900
12:49351316:G:A	acceptor_gain	0.9900
12:49351316:G:GG	acceptor_gain	0.9900
12:49351316:GG:G	acceptor_loss	0.9900
12:49347116:G:GT	donor_gain	0.9800
12:49347552:G:GG	donor_gain	0.9800
12:49347557:G:GT	donor_gain	0.9800
12:49347558:G:T	donor_gain	0.9800
12:49348764:AG:A	acceptor_gain	0.9800
12:49348765:GG:G	acceptor_gain	0.9800
12:49348834:C:T	donor_gain	0.9800
12:49348837:G:GG	donor_gain	0.9800
12:49349713:G:GG	donor_gain	0.9800

AlphaMissense

0 scored. Top likely-pathogenic:

dbSNP variants (sampled 300 via entrez): RS1000174322 (12:49346525 C>G), RS1000204610 (12:49347873 A>C), RS1000278259 (12:49348186 C>A,T), RS1000796956 (12:49351339 C>A,T), RS1001275416 (12:49347298 C>G), RS1001569756 (12:49353823 C>T), RS1001580795 (12:49347560 G>A), RS1001793811 (12:49352762 A>G), RS1001857595 (12:49347837 C>T), RS1002305202 (12:49348666 A>T), RS1002336416 (12:49348999 C>A,G,T), RS1003117365 (12:49346723 T>C), RS1003282586 (12:49349858 G>C), RS1003598785 (12:49350165 C>T), RS1004148071 (12:49348115 C>T)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

6 associations (top):

Study	Trait	p-value
GCST008075_191	HDL cholesterol levels x alcohol consumption (regular vs non-regular drinkers) interaction (2df)	3.000000e-08
GCST008075_73	HDL cholesterol levels x alcohol consumption (regular vs non-regular drinkers) interaction (2df)	1.000000e-08
GCST008084_175	HDL cholesterol levels x alcohol consumption (drinkers vs non-drinkers) interaction (2df)	2.000000e-07
GCST008084_97	HDL cholesterol levels x alcohol consumption (drinkers vs non-drinkers) interaction (2df)	3.000000e-08
GCST008085_171	HDL cholesterol levels in current drinkers	4.000000e-08
GCST008085_93	HDL cholesterol levels in current drinkers	2.000000e-08

EFO canonical traits (2, from GWAS)

EFO ID	Trait name
EFO:0004612	high density lipoprotein cholesterol measurement
EFO:0004329	alcohol drinking

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

26 total (human), top 26 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Tetrachlorodibenzodioxin	affects expression, increases expression	3
sodium arsenite	increases expression, increases abundance	2
Cyclosporine	decreases expression	2
pirinixic acid	affects binding, decreases expression, increases activity	1
2-methyl-4-isothiazolin-3-one	increases expression	1
manganese chloride	increases abundance, increases expression	1
abrine	decreases expression	1
(+)-JQ1 compound	decreases expression	1
Vorinostat	decreases expression	1
Acetaminophen	decreases expression	1
Arsenic	increases abundance, increases expression	1
Benzo(a)pyrene	affects methylation, decreases methylation	1
Estradiol	decreases expression	1
Formaldehyde	increases expression	1
Hydrogen Peroxide	affects expression	1
Manganese	increases abundance, increases expression	1
Niclosamide	increases expression	1
Rotenone	decreases expression	1
Silicon Dioxide	decreases expression	1
Tobacco Smoke Pollution	decreases expression	1
Triclosan	increases expression	1
Valproic Acid	decreases expression	1
1-Methyl-4-phenylpyridinium	decreases expression	1
Cadmium Chloride	decreases expression	1
Copper Sulfate	decreases expression	1
Particulate Matter	increases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.