DNAJC25

Summary

DNAJC25 (DnaJ heat shock protein family (Hsp40) member C25, HGNC:34187) is a protein-coding gene on chromosome 9q31.3, encoding DnaJ homolog subfamily C member 25 (Q9H1X3).

Predicted to be involved in protein folding. Predicted to be located in membrane. Predicted to be active in endoplasmic reticulum membrane.

Source: NCBI Gene 548645 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 16 total
• MANE Select transcript: NM_001015882

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:34187
Approved symbol	DNAJC25
Name	DnaJ heat shock protein family (Hsp40) member C25
Location	9q31.3
Locus type	gene with protein product
Status	Approved
Aliases	bA16L21.2.1
Ensembl gene	ENSG00000059769
Ensembl biotype	protein_coding
Entrez	548645

Gene structure

Transcript identifiers

Ensembl transcripts: 6 — 4 protein_coding, 2 nonsense_mediated_decay

ENST00000313525, ENST00000447096, ENST00000463589, ENST00000905074, ENST00000905075, ENST00000905076

RefSeq mRNA: 1 — MANE Select: NM_001015882 NM_001015882

CCDS: CCDS43862

Canonical transcript exons

ENST00000313525 — 4 exons

Exon	Start	End
ENSE00001519553	111631334	111631743
ENSE00003543457	111653100	111654345
ENSE00003636179	111647107	111647259
ENSE00003670574	111649453	111649923

Expression profiles

Bgee: expression breadth ubiquitous, 256 present calls, max score 96.32.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 7.0045 / max 348.3202, expressed in 1707 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
98016	8.3549	1797
98017	7.0045	1707

Top tissues by expression

259 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
epithelial cell of pancreas	CL:0000083	96.32	gold quality
pancreatic ductal cell	CL:0002079	92.42	gold quality
right lobe of liver	UBERON:0001114	90.78	gold quality
liver	UBERON:0002107	89.81	gold quality
cartilage tissue	UBERON:0002418	87.36	gold quality
germinal epithelium of ovary	UBERON:0001304	86.33	gold quality
left adrenal gland	UBERON:0001234	84.78	gold quality
left ventricle myocardium	UBERON:0006566	84.41	silver quality
left adrenal gland cortex	UBERON:0035825	84.35	gold quality
right adrenal gland cortex	UBERON:0035827	84.08	gold quality
kidney epithelium	UBERON:0004819	84.02	silver quality
calcaneal tendon	UBERON:0003701	84.00	gold quality
right adrenal gland	UBERON:0001233	83.83	gold quality
adrenal cortex	UBERON:0001235	83.76	gold quality
adrenal gland	UBERON:0002369	83.50	gold quality
islet of Langerhans	UBERON:0000006	82.10	gold quality
tibia	UBERON:0000979	81.32	gold quality
stromal cell of endometrium	CL:0002255	80.99	gold quality
parietal pleura	UBERON:0002400	80.97	gold quality
pigmented layer of retina	UBERON:0001782	80.79	gold quality
ileal mucosa	UBERON:0000331	80.62	gold quality
omental fat pad	UBERON:0010414	80.46	gold quality
peritoneum	UBERON:0002358	80.43	gold quality
adrenal tissue	UBERON:0018303	80.34	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	80.05	gold quality
adipose tissue of abdominal region	UBERON:0007808	80.02	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	80.00	gold quality
pancreas	UBERON:0001264	79.74	gold quality
lower esophagus mucosa	UBERON:0035834	79.41	gold quality
adult mammalian kidney	UBERON:0000082	79.08	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	4.39

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

91 targeting DNAJC25, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-340-5P	100.00	72.50	4437
HSA-MIR-3163	100.00	77.23	8605
HSA-MIR-3924	100.00	72.09	2394
HSA-MIR-656-3P	100.00	72.15	2788
HSA-MIR-6758-5P	100.00	66.21	1470
HSA-MIR-5692B	100.00	71.32	2622
HSA-MIR-5692C	100.00	71.32	2622
HSA-MIR-6856-5P	100.00	65.47	1298
HSA-MIR-5692A	100.00	74.40	6850
HSA-MIR-4789-3P	99.99	70.75	2484
HSA-MIR-4282	99.99	75.36	6408
HSA-MIR-4531	99.99	69.70	3181
HSA-MIR-499A-5P	99.98	70.79	1323
HSA-MIR-568	99.98	69.86	2084
HSA-MIR-548AT-5P	99.96	70.83	2666
HSA-MIR-570-3P	99.96	72.41	4910
HSA-MIR-5680	99.91	69.83	3421
HSA-MIR-3671	99.90	73.04	3897
HSA-MIR-374A-5P	99.90	71.34	2923
HSA-MIR-374B-5P	99.90	69.98	2734
HSA-MIR-8087	99.90	69.55	1351
HSA-MIR-576-5P	99.84	70.46	2582
HSA-MIR-4799-5P	99.82	70.60	2663
HSA-MIR-1299	99.77	71.24	2389
HSA-MIR-4517	99.76	69.19	1867
HSA-MIR-7856-5P	99.75	69.99	2901
HSA-MIR-1976	99.74	65.48	1127
HSA-MIR-4306	99.72	70.50	3630
HSA-MIR-4699-3P	99.71	70.15	3142
HSA-MIR-580-3P	99.67	69.23	1841

Cross-species orthologs

8 orthologs

Organism	Symbol	Gene ID
danio_rerio	dnajc25	ENSDARG00000067613
mus_musculus	Dnajc25	ENSMUSG00000070972
rattus_norvegicus	Dnajc25	ENSRNOG00000015340
drosophila_melanogaster	CG7872	FBGN0030658
caenorhabditis_elegans		WBGENE00001020
caenorhabditis_elegans		WBGENE00001025
caenorhabditis_elegans	dnj-28	WBGENE00001046
caenorhabditis_elegans		WBGENE00008122

Paralogs (20): DNAJC11 (ENSG00000007923), DNAJC10 (ENSG00000077232), DNAJC5 (ENSG00000101152), DNAJC3 (ENSG00000102580), DNAJC17 (ENSG00000104129), DNAJC2 (ENSG00000105821), DNAJC12 (ENSG00000108176), DNAJC4 (ENSG00000110011), DNAJC16 (ENSG00000116138), DNAJC14 (ENSG00000135392), DNAJC1 (ENSG00000136770), DNAJC13 (ENSG00000138246), DNAJC5B (ENSG00000147570), DNAJC5G (ENSG00000163793), DNAJC7 (ENSG00000168259), DNAJC21 (ENSG00000168724), DNAJC18 (ENSG00000170464), DNAJC24 (ENSG00000170946), DNAJC30 (ENSG00000176410), DNAJC9 (ENSG00000213551)

Protein

Protein identifiers

DnaJ homolog subfamily C member 25 — Q9H1X3 (reviewed: Q9H1X3)

All UniProt accessions (3): Q9H1X3, F2Z3D1, J3QR79

UniProt curated annotations — full annotation on UniProt →

Subcellular location. Membrane.

Similarity. Belongs to the DNAJC25 family.

Isoforms (3)

UniProt ID	Names	Canonical?
Q9H1X3-1	1	yes
Q9H1X3-2	2
Q9H1X3-3	3

RefSeq proteins (1): NP_001015882* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001623	DnaJ_domain	Domain
IPR036869	J_dom_sf	Homologous_superfamily
IPR044632	DNAJC25-like	Family

Pfam: PF00226

UniProt features (8 total): transmembrane region 3, splice variant 3, chain 1, domain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9H1X3-F1	83.63	0.54

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 90 (showing top): GOBP_PROTEIN_MATURATION, GOBP_PROTEIN_FOLDING, ACTTTAT_MIR1425P, GOCC_NUCLEAR_OUTER_MEMBRANE_ENDOPLASMIC_RETICULUM_MEMBRANE_NETWORK, GOCC_ORGANELLE_SUBCOMPARTMENT, KOINUMA_TARGETS_OF_SMAD2_OR_SMAD3, ALKBH3_TARGET_GENES, DACH1_TARGET_GENES, DIDO1_TARGET_GENES, DROSHA_TARGET_GENES, E2F2_TARGET_GENES, FOXN3_TARGET_GENES, GLI4_TARGET_GENES, KLF7_TARGET_GENES, NKX2_3_TARGET_GENES

GO Biological Process (1): protein folding (GO:0006457)

GO Molecular Function (0):

GO Cellular Component (2): endoplasmic reticulum membrane (GO:0005789), membrane (GO:0016020)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular process	1
protein maturation	1
organelle membrane	1
nuclear outer membrane-endoplasmic reticulum membrane network	1
endoplasmic reticulum subcompartment	1
cellular anatomical structure	1

Protein interactions and networks

STRING

2001 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
DNAJC25	DNAJC9	Q8WXX5	639
DNAJC25	DNAJC8	O75937	585
DNAJC25	DNAJC27	Q9NZQ0	550
DNAJC25	DNAJC12	Q9UKB3	541
DNAJC25	HSPA4	P34932	535
DNAJC25	DNAJC28	Q9NX36	524
DNAJC25	DNAJC22	Q8N4W6	511
DNAJC25	DNAJC15	Q9Y5T4	497
DNAJC25	DNAJC2	Q99543	449
DNAJC25	FBXO36	Q8NEA4	428
DNAJC25	CTXN1	P60606	420
DNAJC25	DNAJC7	Q99615	415
DNAJC25	DNAJC13	O75165	402
DNAJC25	SPATS1	Q496A3	400
DNAJC25	PIH1D2	Q8WWB5	398

IntAct

11 interactions, top by confidence:

A	B	Type	Score
CXCR4	TMEM120B	psi-mi:“MI:0914”(association)	0.530
Sesn2	CASTOR2	psi-mi:“MI:0914”(association)	0.350
M		psi-mi:“MI:0914”(association)	0.350
RAMP2	GXYLT2	psi-mi:“MI:0914”(association)	0.350
CMTM5	TMEM120B	psi-mi:“MI:0914”(association)	0.350
DNAJC25	BRCC3	psi-mi:“MI:0914”(association)	0.350
DNAJC25	TUBAL3	psi-mi:“MI:0914”(association)	0.350
TMEM169	PTGES3L-AARSD1	psi-mi:“MI:0914”(association)	0.350
SLC15A3	GXYLT2	psi-mi:“MI:0914”(association)	0.350
SLC39A14	ESYT2	psi-mi:“MI:0914”(association)	0.350

BioGRID (314): DNAJC25 (Affinity Capture-MS), DNAJC25 (Proximity Label-MS), DNAJC25 (Proximity Label-MS), HAX1 (Affinity Capture-MS), NOP56 (Affinity Capture-MS), YME1L1 (Affinity Capture-MS), PGRMC1 (Affinity Capture-MS), SEC61B (Affinity Capture-MS), DNMT1 (Affinity Capture-MS), EMD (Affinity Capture-MS), PPP6R1 (Affinity Capture-MS), SPG20 (Affinity Capture-MS), SIRT1 (Affinity Capture-MS), RPL13A (Affinity Capture-MS), SRRM2 (Affinity Capture-MS)

ESM2 similar proteins: A1A4P4, A1CH36, A2ALW5, A5AAL8, B0BN56, O42911, O70279, P0C2B7, P58468, P83565, Q0CLE8, Q1ECT8, Q290P4, Q2GVC2, Q3SZ86, Q4G0I0, Q4V7Q1, Q5B4U6, Q5BJW9, Q5RFR4, Q5XJW2, Q61733, Q6RUT7, Q753F1, Q7SDU5, Q7SHR9, Q80ZS3, Q86TS9, Q8BGX2, Q8BK72, Q8CHP5, Q8SPE7, Q8TAE8, Q8VD26, Q8WUQ7, Q96AN5, Q96DF8, Q9BRP8, Q9BSF4, Q9BYN8

Diamond homologs: A0LJ41, A1V9Q3, A1WAR7, A1WX30, A2ALW5, A3DF24, A4IR30, A5ITA7, A5WBF8, A6QHC2, A6U251, A7GT07, A7X2Y0, A7Z6W0, A8FFD1, A8Z4B8, A9KE65, A9NDK6, A9VHU0, B0JW23, B0VQ00, B1HUD0, B1WVR2, B1Y787, B1YKT0, B2GBQ6, B2I2G6, B2J3J3, B3EE31, B7GV08, B7HPL2, B7I2B2, B7JN38, B8CXL0, B8FUN3, B9DNJ9, B9IY80, C1ESK7, C3L5R6, C3P8L9

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

16 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	16
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1015 predictions. Top by Δscore:

Variant	Effect	Δscore
9:111631740:CAAGG:C	donor_loss	1.0000
9:111631741:AAGGT:A	donor_loss	1.0000
9:111631742:AGG:A	donor_loss	1.0000
9:111631743:GGT:G	donor_loss	1.0000
9:111631744:G:GA	donor_loss	1.0000
9:111631745:T:A	donor_loss	1.0000
9:111631745:T:G	donor_loss	1.0000
9:111632592:G:GT	donor_gain	1.0000
9:111647101:TTACA:T	acceptor_loss	1.0000
9:111647102:TACA:T	acceptor_loss	1.0000
9:111647103:A:AG	acceptor_gain	1.0000
9:111647104:CA:C	acceptor_loss	1.0000
9:111647104:CAGGA:C	acceptor_loss	1.0000
9:111647105:A:AG	acceptor_gain	1.0000
9:111647105:AG:A	acceptor_gain	1.0000
9:111647105:AGG:A	acceptor_loss	1.0000
9:111647106:G:GA	acceptor_gain	1.0000
9:111647106:G:GG	acceptor_gain	1.0000
9:111647106:GG:G	acceptor_gain	1.0000
9:111647106:GGAT:G	acceptor_gain	1.0000
9:111647258:AGG:A	donor_loss	1.0000
9:111647259:GGT:G	donor_loss	1.0000
9:111647260:G:GA	donor_loss	1.0000
9:111647260:G:GG	donor_gain	1.0000
9:111647261:T:G	donor_loss	1.0000
9:111649451:A:AG	acceptor_gain	1.0000
9:111649452:G:GT	acceptor_gain	1.0000
9:111649452:GT:G	acceptor_gain	1.0000
9:111649452:GTT:G	acceptor_gain	1.0000
9:111649615:G:GT	donor_gain	1.0000

AlphaMissense

2347 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
9:111631739:T:A	L111H	0.999
9:111631708:T:C	F101L	0.998
9:111631710:C:A	F101L	0.998
9:111631710:C:G	F101L	0.998
9:111647120:G:C	R117P	0.998
9:111647233:A:C	S155R	0.998
9:111647235:C:A	S155R	0.998
9:111647235:C:G	S155R	0.998
9:111649517:G:C	R185P	0.998
9:111631636:C:G	H77D	0.997
9:111631727:C:A	A107D	0.997
9:111631739:T:C	L111P	0.997
9:111649525:G:C	A188P	0.997
9:111653174:A:C	R345S	0.997
9:111653174:A:T	R345S	0.997
9:111631612:T:G	Y69D	0.996
9:111631625:C:A	A73D	0.996
9:111649538:C:A	A192D	0.996
9:111649903:T:A	W314R	0.996
9:111649903:T:C	W314R	0.996
9:111653173:G:C	R345T	0.996
9:111631555:T:C	C50R	0.995
9:111631557:C:G	C50W	0.995
9:111631638:C:A	H77Q	0.995
9:111631638:C:G	H77Q	0.995
9:111631709:T:G	F101C	0.995
9:111631720:G:C	A105P	0.995
9:111649526:C:A	A188D	0.995
9:111653171:G:C	R344S	0.995
9:111653171:G:T	R344S	0.995

dbSNP variants (sampled 300 via entrez): RS1000459009 (9:111634178 G>A), RS1000810321 (9:111634482 G>A,C), RS1000841848 (9:111654599 G>A,T), RS1000843696 (9:111653449 T>G), RS1000915842 (9:111647010 T>C), RS1001120179 (9:111647534 C>T), RS1001205123 (9:111637945 T>C), RS1001210309 (9:111646770 C>T), RS1001346544 (9:111643433 C>A,T), RS1001357782 (9:111646006 G>T), RS1001397442 (9:111643134 C>T), RS1001429589 (9:111646423 T>C), RS1001981861 (9:111634892 A>G), RS1002172500 (9:111648375 C>T), RS1002300412 (9:111642436 A>C)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

21 total (human), top 21 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
sodium arsenite	increases abundance, increases expression	2
Cyclosporine	increases expression	2
GSK-J4	increases expression	1
triphenyl phosphate	affects expression	1
glycidyl methacrylate	decreases expression	1
zinc chromate	increases abundance, increases expression	1
chromium hexavalent ion	increases abundance, increases expression	1
2-palmitoylglycerol	increases expression	1
K 7174	increases expression	1
abrine	increases expression	1
PCI 5002	affects cotreatment, increases expression	1
Air Pollutants	decreases expression, increases abundance	1
Arsenic	increases abundance, increases expression	1
Benzo(a)pyrene	decreases expression	1
Thiram	increases expression	1
Tobacco Smoke Pollution	increases expression	1
Tunicamycin	increases expression	1
Valproic Acid	increases expression	1
Zinc	affects cotreatment, increases expression	1
Aflatoxin B1	decreases expression, decreases methylation	1
Particulate Matter	decreases expression, increases abundance	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.