DNPEP

Summary

DNPEP (aspartyl aminopeptidase, HGNC:2981) is a protein-coding gene on chromosome 2q35, encoding Aspartyl aminopeptidase (Q9ULA0). Aminopeptidase with specificity towards an acidic amino acid at the N-terminus.

The protein encoded by this gene is an aminopeptidase which prefers acidic amino acids, and specifically favors aspartic acid over glutamic acid. It is thought to be a cytosolic protein involved in general metabolism of intracellular proteins. Several transcript variants encoding different isoforms have been found for this gene.

Source: NCBI Gene 23549 — RefSeq curated summary.

At a glance

• GWAS associations: 4
• Clinical variants (ClinVar): 99 total
• Druggable target: yes
• MANE Select transcript: NM_012100

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:2981
Approved symbol	DNPEP
Name	aspartyl aminopeptidase
Location	2q35
Locus type	gene with protein product
Status	Approved
Aliases	DAP, ASPEP
Ensembl gene	ENSG00000123992
Ensembl biotype	protein_coding
OMIM	611367
Entrez	23549

Gene structure

Transcript identifiers

Ensembl transcripts: 30 — 20 protein_coding, 6 retained_intron, 3 nonsense_mediated_decay, 1 protein_coding_CDS_not_defined

ENST00000273075, ENST00000322176, ENST00000373963, ENST00000373966, ENST00000373972, ENST00000429013, ENST00000430206, ENST00000434339, ENST00000457935, ENST00000460963, ENST00000462779, ENST00000488051, ENST00000488881, ENST00000490371, ENST00000519698, ENST00000519905, ENST00000520694, ENST00000521459, ENST00000522421, ENST00000523282, ENST00000523527, ENST00000851982, ENST00000851983, ENST00000851984, ENST00000851985, ENST00000851986, ENST00000925428, ENST00000964369, ENST00000964370, ENST00000964371

RefSeq mRNA: 8 — MANE Select: NM_012100 NM_001319116, NM_001319117, NM_001319118, NM_001319119, NM_001319120, NM_001319121, NM_001319122, NM_012100

CCDS: CCDS42823, CCDS82574, CCDS86923

Canonical transcript exons

ENST00000273075 — 15 exons

Exon	Start	End
ENSE00001224311	219387759	219387912
ENSE00001771788	219372043	219374342
ENSE00003460921	219385968	219386098
ENSE00003479336	219381979	219382139
ENSE00003489559	219385424	219385531
ENSE00003494903	219386665	219386778
ENSE00003508066	219385631	219385706
ENSE00003511866	219381545	219381584
ENSE00003527259	219386286	219386411
ENSE00003570789	219387070	219387163
ENSE00003579022	219386892	219386980
ENSE00003585229	219374855	219375022
ENSE00003594018	219384366	219384443
ENSE00003651325	219381335	219381436
ENSE00003667122	219383131	219383214

Expression profiles

Bgee: expression breadth ubiquitous, 295 present calls, max score 97.92.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 19.7917 / max 114.2033, expressed in 1808 samples.

FANTOM5 promoters (6 alternative TSS)

Promoter ID	TPM avg	Samples expressed
34078	11.4917	1793
34076	6.0231	1666
34077	1.6437	1209
34074	0.3943	197
34075	0.2314	113
34079	0.0075	3

Top tissues by expression

299 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
left testis	UBERON:0004533	97.92	gold quality
right testis	UBERON:0004534	97.90	gold quality
endocervix	UBERON:0000458	97.57	gold quality
mucosa of stomach	UBERON:0001199	97.49	gold quality
apex of heart	UBERON:0002098	97.30	gold quality
small intestine Peyer’s patch	UBERON:0003454	97.18	gold quality
mucosa of transverse colon	UBERON:0004991	97.03	gold quality
right ovary	UBERON:0002118	96.93	gold quality
left uterine tube	UBERON:0001303	96.91	gold quality
upper lobe of left lung	UBERON:0008952	96.90	gold quality
ectocervix	UBERON:0012249	96.87	gold quality
transverse colon	UBERON:0001157	96.86	gold quality
left ovary	UBERON:0002119	96.78	gold quality
skin of leg	UBERON:0001511	96.70	gold quality
body of uterus	UBERON:0009853	96.69	gold quality
skin of abdomen	UBERON:0001416	96.61	gold quality
small intestine	UBERON:0002108	96.54	gold quality
gastrocnemius	UBERON:0001388	96.51	gold quality
right lung	UBERON:0002167	96.40	gold quality
omental fat pad	UBERON:0010414	96.37	gold quality
body of stomach	UBERON:0001161	96.36	gold quality
peritoneum	UBERON:0002358	96.34	gold quality
lower esophagus mucosa	UBERON:0035834	96.34	gold quality
muscle layer of sigmoid colon	UBERON:0035805	96.32	gold quality
right coronary artery	UBERON:0001625	96.30	gold quality
lower esophagus	UBERON:0013473	96.24	gold quality
lower esophagus muscularis layer	UBERON:0035833	96.24	gold quality
sperm	CL:0000019	96.23	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	96.22	gold quality
metanephros cortex	UBERON:0010533	96.18	gold quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	7.53
E-CURD-112	no	3.95
E-MTAB-5061	no	3.12

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

71 targeting DNPEP, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-656-3P	100.00	72.15	2788
HSA-LET-7A-3P	100.00	74.03	3932
HSA-LET-7B-3P	100.00	74.08	3913
HSA-LET-7F-1-3P	100.00	74.02	3928
HSA-MIR-98-3P	100.00	74.08	3907
HSA-MIR-4533	100.00	69.48	2758
HSA-MIR-8485	100.00	77.57	4731
HSA-MIR-4789-3P	99.99	70.75	2484
HSA-MIR-150-5P	99.99	66.69	1976
HSA-MIR-4282	99.99	75.36	6408
HSA-MIR-4789-5P	99.98	70.76	2721
HSA-MIR-3688-3P	99.97	72.02	2834
HSA-MIR-4666A-3P	99.96	71.71	3434
HSA-MIR-6778-3P	99.96	67.29	2693
HSA-MIR-548AA	99.96	70.64	3753
HSA-MIR-548AP-3P	99.96	70.64	3753
HSA-MIR-548T-3P	99.96	70.64	3753
HSA-MIR-9983-3P	99.94	71.48	3631
HSA-MIR-4302	99.89	67.94	1187
HSA-MIR-95-5P	99.89	72.17	3973
HSA-MIR-129-5P	99.88	70.26	3273
HSA-MIR-4728-5P	99.85	69.39	4718
HSA-MIR-6785-5P	99.82	68.68	4428
HSA-MIR-6515-3P	99.82	68.19	1933
HSA-MIR-2052	99.79	69.37	2031
HSA-MIR-8076	99.78	68.52	1170
HSA-MIR-4524A-3P	99.72	66.85	2406
HSA-MIR-149-3P	99.72	68.22	3963
HSA-MIR-33A-3P	99.70	70.27	3362
HSA-MIR-6883-5P	99.69	68.05	3785

Literature-anchored findings (GeneRIF, showing 4)

• Identification of histidine residues important in the catalysis and structure of aspartyl aminopeptidase (PMID:12413488)
• The crystal structure of human DNPEP complexed with zinc and a substrate analogue aspartate-beta-hydroxamate reveals a dodecameric machinery built by domain-swapped dimers. (PMID:22720794)
• aspartyl aminopeptidase was upregulated in colorectal cancer tissues, and greater activity correlated significantly with the absence of lymph node metastases and with better overall survival (PMID:23948443)
• Aspartyl Aminopeptidase Suppresses Proliferation, Invasion, and Stemness of Breast Cancer Cells via Targeting CD44. (PMID:31228326)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	dnpep	ENSDARG00000022599
mus_musculus	Dnpep	ENSMUSG00000026209
rattus_norvegicus	Dnpep	ENSRNOG00000019772

Protein

Protein identifiers

Aspartyl aminopeptidase — Q9ULA0 (reviewed: Q9ULA0)

All UniProt accessions (14): B9ZVU2, C9J1E2, C9JBE1, C9JRG3, E3W974, E3W979, E5RHR3, E5RIA4, E5RJ35, E7EMB6, E7EPX3, Q9ULA0, E7ETB3, F8WAN0

UniProt curated annotations — full annotation on UniProt →

Function. Aminopeptidase with specificity towards an acidic amino acid at the N-terminus. Likely to play an important role in intracellular protein and peptide metabolism.

Subunit / interactions. Tetrahedron-shaped homododecamer built from six homodimers.

Subcellular location. Cytoplasm.

Tissue specificity. Ubiquitous.

Activity regulation. One of the zinc ions is readily exchangeable with other divalent cations such as manganese, which strongly stimulates the enzymatic activity.

Cofactor. Binds 2 Zn(2+) ions per subunit.

Similarity. Belongs to the peptidase M18 family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q9ULA0-1	1	yes
Q9ULA0-2	2

RefSeq proteins (8): NP_001306045, NP_001306046, NP_001306047, NP_001306048, NP_001306049, NP_001306050, NP_001306051, NP_036232* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001948	Peptidase_M18	Family
IPR023358	Peptidase_M18_dom2	Homologous_superfamily

Pfam: PF02127

Enzyme classification (BRENDA):

• EC 3.4.11.21 — aspartyl aminopeptidase (BRENDA: 22 organisms, 65 substrates, 174 inhibitors, 26 Km, 4 kcat entries)

Substrate kinetics (BRENDA)

13 substrates with measured Km, best-characterized 13. Km ranges are aggregated across organisms/conditions.

Substrate	Km (mM)	Measurements
L-ASP-4-NITROANILIDE	0.092–1.21	4
ASP-4-NITROANILIDE	0.389–5.93	3
GLU-4-NITROANILIDE	0.137–1.05	3
ASP-7-AMIDO-4-METHYLCOUMARIN	0.327–0.385	2
ASP-ALA-PRO-SULFAMETHOXAZOLE	2–2.3	2
GLU-7-AMIDO-4-METHYLCOUMARIN	0.135–0.136	2
L-GLU-4-NITROANILIDE	0.19–25.2	2
ANGIOTENSIN I	0.064	1
ANGIOTENSIN II	0.25	1
ASP-ALA-PRO-NAPHTHYLAMIDE	2	1
ASP-XAA	0.94	1
GLU 2-NAPHTHYLAMIDE	0.35	1
GLU-ALA-PRO-NAPHTHYLAMIDE	8.4	1

UniProt features (61 total): strand 18, helix 16, binding site 14, turn 7, modified residue 2, sequence conflict 2, chain 1, splice variant 1

Structure

Experimental structures (PDB)

1 structures.

PDB	Method	Resolution (Å)
4DYO	X-RAY DIFFRACTION	2.2

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9ULA0-F1	95.95	0.94

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (14): 104; 356; 359; 384; 391; 450; 180; 274; 274; 311; 311; 312 …

Post-translational modifications (2): 213, 1

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 0 (showing top):

GO Biological Process (2): proteolysis (GO:0006508), peptide metabolic process (GO:0006518)

GO Molecular Function (8): aminopeptidase activity (GO:0004177), metallopeptidase activity (GO:0008237), zinc ion binding (GO:0008270), identical protein binding (GO:0042802), protein binding (GO:0005515), peptidase activity (GO:0008233), hydrolase activity (GO:0016787), metal ion binding (GO:0046872)

GO Cellular Component (4): nucleus (GO:0005634), cytoplasm (GO:0005737), cytosol (GO:0005829), blood microparticle (GO:0072562)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	3
protein metabolic process	1
metabolic process	1
exopeptidase activity	1
peptidase activity	1
transition metal ion binding	1
protein binding	1
binding	1
hydrolase activity	1
catalytic activity, acting on a protein	1
catalytic activity	1
cation binding	1
intracellular membrane-bounded organelle	1
intracellular anatomical structure	1
cytoplasm	1
extracellular region	1

Protein interactions and networks

STRING

1491 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
DNPEP	DPP4	P27487	841
DNPEP	RNPEP	Q9H4A4	756
DNPEP	ENPEP	Q07075	711
DNPEP	LAP3	P28838	689
DNPEP	PGPEP1	Q9NXJ5	598
DNPEP	ANPEP	P15144	585
DNPEP	METAP1	P53582	558
DNPEP	LNPEP	Q9UIQ6	557
DNPEP	NPEPPS	P55786	557
DNPEP	PREP	P48147	525
DNPEP	XPNPEP3	Q9NQH7	521
DNPEP	DPP3	Q9NY33	484
DNPEP	LRRC34	Q8IZ02	464
DNPEP	DUS2	Q9NX74	463
DNPEP	XPNPEP1	Q9NQW7	460

IntAct

105 interactions, top by confidence:

A	B	Type	Score
DNPEP	LNX1	psi-mi:“MI:0915”(physical association)	0.780
LNX1	DNPEP	psi-mi:“MI:0915”(physical association)	0.780
MPP1	DNPEP	psi-mi:“MI:0915”(physical association)	0.720
DNPEP	MPP1	psi-mi:“MI:0915”(physical association)	0.720
CFTR	ESYT2	psi-mi:“MI:0914”(association)	0.710
DNPEP	DNPEP	psi-mi:“MI:0915”(physical association)	0.670
MPPED1	TXNDC9	psi-mi:“MI:0914”(association)	0.640
RAB11B	SH3BP5	psi-mi:“MI:0914”(association)	0.640
GPX7	GAK	psi-mi:“MI:0914”(association)	0.640
MGME1	POLG	psi-mi:“MI:0914”(association)	0.640
DNPEP	SCAF8	psi-mi:“MI:0915”(physical association)	0.590
TAE1	DNPEP	psi-mi:“MI:0915”(physical association)	0.560
DNPEP	TAE1	psi-mi:“MI:0915”(physical association)	0.560
RAB11A	SH3BP5	psi-mi:“MI:0914”(association)	0.530

BioGRID (112): DNPEP (Two-hybrid), DNPEP (Two-hybrid), LNX1 (Two-hybrid), DNPEP (Affinity Capture-MS), DNPEP (Affinity Capture-MS), DNPEP (Two-hybrid), ADAMTSL4 (Two-hybrid), DNPEP (Two-hybrid), TPI1 (Co-fractionation), DNPEP (Affinity Capture-MS), DNPEP (Affinity Capture-MS), DNPEP (Affinity Capture-MS), DNPEP (Affinity Capture-MS), DNPEP (Affinity Capture-MS), DNPEP (Affinity Capture-MS)

ESM2 similar proteins: A0AVT1, A0JMS7, A2BP19, A6H630, B0C2K7, B3EK39, B3MF31, B4MNL1, B4P925, B4S3A5, P17812, P46446, P50547, P59830, P70698, P72208, Q10VR3, Q1LXS2, Q1RMS2, Q28C61, Q2NL24, Q3MC79, Q58EM4, Q5BJ91, Q5F3Z1, Q5M876, Q5RBT2, Q5XGC5, Q6AXB1, Q6AYT5, Q6DHI0, Q6DHQ3, Q6DJA3, Q7SYV1, Q7TS68, Q8C7R4, Q8R3P0, Q8YQC1, Q91XE4, Q969U7

Diamond homologs: A0PYH7, A1KGT3, A4VJG1, A4XRN0, A5U0I9, A5W7K3, A6V2H4, B0KTU0, B1J1S3, B7V7X2, B9RAJ0, C1ALD5, C1DQM8, C3K0D7, O36014, O51572, P38821, P59951, P9WHT0, P9WHT1, Q02Q78, Q19087, Q1IDE6, Q2HJH1, Q2UPZ7, Q3KFM3, Q48L80, Q4ZW15, Q50022, Q54M70, Q5RBT2, Q82F74, Q87YC5, Q88M44, Q97LF4, Q9HYZ3, Q9ULA0, Q9XA76, Q9Z2W0, P14904

SIGNOR signaling

2 interactions.

A	Effect	B	Mechanism
PAK5	“down-regulates quantity by destabilization”	DNPEP	phosphorylation
DNPEP	“down-regulates quantity by destabilization”	USP4	cleavage

Disease & clinical

Clinical variants and AI predictions

ClinVar

99 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	80
Likely benign	2
Benign	2

Top pathogenic / likely-pathogenic (0)

SpliceAI

2173 predictions. Top by Δscore:

Variant	Effect	Δscore
2:219374849:TGTTA:T	donor_loss	1.0000
2:219374850:GTTAC:G	donor_loss	1.0000
2:219374851:TTACC:T	donor_loss	1.0000
2:219374852:TACCT:T	donor_loss	1.0000
2:219374853:A:AG	donor_loss	1.0000
2:219374854:CCT:C	donor_loss	1.0000
2:219375021:TC:T	acceptor_gain	1.0000
2:219375021:TCC:T	acceptor_loss	1.0000
2:219375022:CC:C	acceptor_gain	1.0000
2:219375022:CCTA:C	acceptor_loss	1.0000
2:219375023:C:CA	acceptor_loss	1.0000
2:219375023:C:CC	acceptor_gain	1.0000
2:219375024:T:C	acceptor_loss	1.0000
2:219381331:TCAC:T	donor_loss	1.0000
2:219381332:CACC:C	donor_loss	1.0000
2:219381333:A:AG	donor_loss	1.0000
2:219381334:CCTGC:C	donor_loss	1.0000
2:219381434:GCCC:G	acceptor_loss	1.0000
2:219381435:CC:C	acceptor_gain	1.0000
2:219381436:CC:C	acceptor_gain	1.0000
2:219381437:C:CC	acceptor_gain	1.0000
2:219381438:T:A	acceptor_loss	1.0000
2:219381539:TCTCA:T	donor_loss	1.0000
2:219381540:CTCAC:C	donor_loss	1.0000
2:219381541:TCACC:T	donor_loss	1.0000
2:219381542:CA:C	donor_loss	1.0000
2:219381544:C:T	donor_loss	1.0000
2:219383221:C:CT	acceptor_gain	1.0000
2:219383222:A:T	acceptor_gain	1.0000
2:219384441:GACCT:G	acceptor_loss	1.0000

AlphaMissense

3166 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
2:219384397:T:A	D274V	0.999
2:219382009:T:A	D356V	0.998
2:219382014:G:C	S354R	0.998
2:219382014:G:T	S354R	0.998
2:219382016:T:G	S354R	0.998
2:219384396:G:C	D274E	0.998
2:219384396:G:T	D274E	0.998
2:219384397:T:G	D274A	0.998
2:219374901:T:A	E454V	0.997
2:219382008:G:C	D356E	0.997
2:219382008:G:T	D356E	0.997
2:219382009:T:G	D356A	0.997
2:219383135:T:A	E311V	0.997
2:219384381:G:C	C279W	0.997
2:219384393:A:C	N275K	0.997
2:219384393:A:T	N275K	0.997
2:219386330:A:G	W139R	0.997
2:219386330:A:T	W139R	0.997
2:219386940:G:C	F57L	0.997
2:219386940:G:T	F57L	0.997
2:219386942:A:G	F57L	0.997
2:219374904:C:G	R453P	0.996
2:219383134:T:A	E311D	0.996
2:219383134:T:G	E311D	0.996
2:219386320:C:G	R142P	0.996
2:219386915:A:G	W66R	0.996
2:219386915:A:T	W66R	0.996
2:219374991:C:T	G424E	0.995
2:219382010:C:G	D356H	0.995
2:219384398:C:G	D274H	0.995

dbSNP variants (sampled 300 via entrez): RS1000186868 (2:219400070 T>C,G), RS1000288318 (2:219399979 C>G), RS1000337377 (2:219393679 C>T), RS1000361531 (2:219399703 A>G), RS1000384700 (2:219400333 C>T), RS1000389019 (2:219380666 C>G,T), RS1000517941 (2:219392155 A>C,G), RS1000634587 (2:219393749 C>G), RS1001000552 (2:219375433 G>C), RS1001039529 (2:219381726 A>G,T), RS1001167586 (2:219401270 T>C), RS1001231348 (2:219395070 C>T), RS1001354802 (2:219401075 G>A), RS1001510504 (2:219375623 G>A), RS1001596618 (2:219376820 G>A,C)

Disease associations

OMIM: gene MIM:611367 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

4 associations (top):

Study	Trait	p-value
GCST002069_2	Systemic lupus erythematosus and Systemic sclerosis	2.000000e-07
GCST008839_309	Height	2.000000e-09
GCST009733_76	Urinary metabolite levels in chronic kidney disease	4.000000e-11
GCST011742_24	Triglyceride levels in HIV infection	8.000000e-06

EFO canonical traits (2, from GWAS)

EFO ID	Trait name
EFO:0005116	urinary metabolite measurement
EFO:0004530	triglyceride measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (2): CHEMBL2761 (SINGLE PROTEIN), CHEMBL3831223 (PROTEIN FAMILY)

PharmGKB: 1 entry (VIP=true, CPIC=false)

GtoPdb / IUPHAR curated pharmacology

(IUPHAR/BPS Guide to Pharmacology — expert-curated)

Target class: enzyme — M18: Aminopeptidase I

Most potent curated ligand interactions (1 total), top 1:

Ligand	Action	Affinity	Parameter
compound 9 [PMID: 1738140]	Inhibition	6.29	pKi

ChEMBL bioactivities

6 potent at pChembl≥5 of 7 total, top 6 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
6.29	Ki	510	nM	CHEMBL309130
6.00	Ki	1000	nM	CHEMBL143589
5.82	Kd	1530	nM	CHEMBL5653589
5.82	ED50	1530	nM	CHEMBL5653589
5.72	Ki	1900	nM	CHEMBL140846
5.27	Ki	5400	nM	CHEMBL142010

PubChem BioAssay actives

5 with measured affinity, of 19 total; 5 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
(2S,3R)-3-amino-2-hydroxy-N-(3-methylbutyl)-4-phenylbutanamide	35693: Compound was tested for binding affinity towards cytosolic aminopeptidase	ki	0.5100	uM
3-amino-N-(3-methylbutyl)-2-oxo-4-phenylbutanamide	35693: Compound was tested for binding affinity towards cytosolic aminopeptidase	ki	1.0000	uM
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2148256: Binding affinity to human DNPEP incubated for 45 mins by Kinobead based pull down assay	kd	1.5296	uM
(3S)-3-amino-5-methyl-N-(3-methylbutyl)-2-oxohexanamide	35693: Compound was tested for binding affinity towards cytosolic aminopeptidase	ki	1.9000	uM
(2S,3R)-3-amino-2-hydroxy-5-methyl-N-(3-methylbutyl)hexanamide	55259: Compound was tested for binding affinity towards Cytosolic aminopeptidase	ki	5.4000	uM

CTD chemical–gene interactions

34 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
methacrylaldehyde	affects cotreatment, decreases expression, increases oxidation, increases abundance	2
Acrolein	decreases expression, increases oxidation, increases abundance, affects cotreatment	2
Ozone	affects cotreatment, decreases expression, increases oxidation, increases abundance	2
Cadmium Chloride	increases expression	2
alpha-pinene	increases oxidation, increases abundance, affects cotreatment, decreases expression	1
pirinixic acid	increases expression, affects binding, increases activity	1
beta-lapachone	increases expression	1
sodium arsenite	affects expression	1
cobaltous chloride	increases expression	1
potassium chromate(VI)	affects cotreatment, decreases expression	1
epigallocatechin gallate	affects cotreatment, decreases expression	1
perfluorooctane sulfonic acid	increases expression	1
nutlin 3	affects cotreatment, increases secretion	1
ICG 001	increases expression	1
3-(2-hydroxy-4-(2-methylnonan-2-yl)phenyl)cyclohexan-1-ol	increases expression	1
Air Pollutants	affects cotreatment, decreases expression, increases abundance, increases oxidation	1
Atrazine	increases expression	1
Vehicle Emissions	increases abundance, increases expression	1
Benzo(a)pyrene	decreases methylation	1
Bilirubin	decreases expression	1
Dactinomycin	affects cotreatment, increases secretion	1
Doxorubicin	decreases expression	1
Ivermectin	decreases expression	1
Lead	decreases expression	1
Rotenone	decreases expression	1
Selenium	increases expression	1
Smoke	decreases expression	1
Urethane	decreases expression	1
Valproic Acid	decreases expression	1
Cyclosporine	increases expression	1

ChEMBL screening assays

11 unique, capped per target: 10 binding, 1 admet

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL4118714	Binding	Binding affinity to DNPEP in human NCI-H23 cells at 1 uM by mass spectrometry based pull down assay	Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors. — Bioorg Med Chem
CHEMBL4334276	ADMET	Stability in pH 2 HCl assessed as aminopeptidase (unknown origin)-mediated compound hydrolysis by measuring parent compound remaining at 200 uM up to 6 hrs by RP-HPLC analysis	Astratides: Insulin-Modulating, Insecticidal, and Antifungal Cysteine-Rich Peptides from Astragalus membranaceus. — J Nat Prod

Cellosaurus cell lines

3 cell lines: 3 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_D1MA	Abcam K-562 DNPEP KO	Cancer cell line	Female
CVCL_D2IV	Abcam Raji DNPEP KO	Cancer cell line	Male
CVCL_UQ42	Abcam Jurkat DNPEP KO	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.