DSTN

Summary

DSTN (destrin, actin depolymerizing factor, HGNC:15750) is a protein-coding gene on chromosome 20p12.1, encoding Destrin (P60981). Actin-depolymerizing protein. It is a selective cancer dependency (DepMap: 10.2% of cell lines).

The product of this gene belongs to the actin-binding proteins ADF family. This family of proteins is responsible for enhancing the turnover rate of actin in vivo. This gene encodes the actin depolymerizing protein that severs actin filaments (F-actin) and binds to actin monomers (G-actin). Two transcript variants encoding distinct isoforms have been identified for this gene.

Source: NCBI Gene 11034 — RefSeq curated summary.

At a glance

• GWAS associations: 2
• Clinical variants (ClinVar): 17 total
• Druggable target: yes
• Cancer dependency (DepMap): dependent in 10.2% of screened cell lines
• MANE Select transcript: NM_006870

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:15750
Approved symbol	DSTN
Name	destrin, actin depolymerizing factor
Location	20p12.1
Locus type	gene with protein product
Status	Approved
Aliases	ADF, ACTDP
Ensembl gene	ENSG00000125868
Ensembl biotype	protein_coding
OMIM	609114
Entrez	11034

Gene structure

Transcript identifiers

Ensembl transcripts: 7 — 6 protein_coding, 1 nonsense_mediated_decay

ENST00000246069, ENST00000449141, ENST00000474024, ENST00000888381, ENST00000925115, ENST00000925116, ENST00000955166

RefSeq mRNA: 2 — MANE Select: NM_006870 NM_001011546, NM_006870

CCDS: CCDS13127, CCDS46580

Canonical transcript exons

ENST00000246069 — 4 exons

Exon	Start	End
ENSE00001710202	17570075	17570211
ENSE00003459031	17607037	17609919
ENSE00003493706	17600738	17601045
ENSE00003702589	17604555	17604631

Expression profiles

Bgee: expression breadth ubiquitous, 304 present calls, max score 99.97.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 287.5698 / max 2127.3404, expressed in 1825 samples.

FANTOM5 promoters (6 alternative TSS)

Promoter ID	TPM avg	Samples expressed
183652	273.7708	1825
183653	9.4144	1631
183649	3.1281	1071
183651	0.5376	244
183650	0.3805	198
183648	0.3385	203

Top tissues by expression

304 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
blood vessel layer	UBERON:0004797	99.97	gold quality
saphenous vein	UBERON:0007318	99.97	gold quality
urethra	UBERON:0000057	99.95	gold quality
popliteal artery	UBERON:0002250	99.93	gold quality
tibial artery	UBERON:0007610	99.93	gold quality
right coronary artery	UBERON:0001625	99.92	gold quality
artery	UBERON:0001637	99.92	gold quality
aorta	UBERON:0000947	99.91	gold quality
descending thoracic aorta	UBERON:0002345	99.90	gold quality
vena cava	UBERON:0004087	99.90	gold quality
ascending aorta	UBERON:0001496	99.88	gold quality
thoracic aorta	UBERON:0001515	99.88	gold quality
superficial temporal artery	UBERON:0001614	99.87	gold quality
cauda epididymis	UBERON:0004360	99.86	gold quality
lower esophagus muscularis layer	UBERON:0035833	99.85	gold quality
seminal vesicle	UBERON:0000998	99.84	gold quality
coronary artery	UBERON:0001621	99.84	gold quality
lower esophagus	UBERON:0013473	99.84	gold quality
left coronary artery	UBERON:0001626	99.83	gold quality
esophagogastric junction muscularis propria	UBERON:0035841	99.81	gold quality
bronchial epithelial cell	CL:0002328	99.80	gold quality
mucosa of stomach	UBERON:0001199	99.80	gold quality
lower lobe of lung	UBERON:0008949	99.80	gold quality
pons	UBERON:0000988	99.79	gold quality
adult organism	UBERON:0007023	99.79	gold quality
smooth muscle tissue	UBERON:0001135	99.77	gold quality
cardia of stomach	UBERON:0001162	99.77	gold quality
muscle layer of sigmoid colon	UBERON:0035805	99.76	gold quality
penis	UBERON:0000989	99.74	gold quality
pylorus	UBERON:0001166	99.73	gold quality

Single-cell (SCXA)

Detected in 29 experiment(s), a significant marker in 25.

Experiment	Marker?	Max mean expression
E-CURD-126	yes	6642.58
E-HCAD-36	yes	5332.53
E-MTAB-10287	yes	5048.57
E-MTAB-10885	yes	4601.43
E-MTAB-8410	yes	4509.24
E-MTAB-8322	yes	4158.30
E-HCAD-13	yes	3075.68
E-MTAB-8381	yes	2944.65
E-MTAB-8142	yes	2721.45
E-MTAB-9906	yes	2218.02
E-HCAD-1	yes	211.24
E-GEOD-134144	yes	34.79
E-GEOD-137537	yes	31.31
E-MTAB-6678	yes	28.74
E-CURD-46	yes	28.26

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): ZNF331

miRNA regulators (miRDB)

71 targeting DSTN, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-4668-3P	100.00	68.74	2635
HSA-MIR-656-3P	100.00	72.15	2788
HSA-MIR-186-5P	99.99	70.83	3707
HSA-MIR-1250-3P	99.96	70.04	4038
HSA-MIR-1236-3P	99.94	68.04	1695
HSA-MIR-335-3P	99.93	73.36	4958
HSA-MIR-3143	99.93	71.96	3104
HSA-MIR-10527-5P	99.91	72.28	3754
HSA-MIR-4753-3P	99.90	71.03	3786
HSA-MIR-95-5P	99.89	72.17	3973
HSA-MIR-7162-3P	99.89	68.16	1682
HSA-MIR-153-5P	99.89	73.86	6317
HSA-MIR-5582-3P	99.86	72.48	4221
HSA-MIR-6079	99.84	68.54	1170
HSA-MIR-6875-3P	99.82	70.26	2983
HSA-MIR-3133	99.81	70.92	3506
HSA-MIR-4760-5P	99.80	69.88	1619
HSA-MIR-448	99.79	72.37	2103
HSA-MIR-548AJ-5P	99.78	71.12	3085
HSA-MIR-548F-5P	99.78	71.02	3093
HSA-MIR-548G-5P	99.78	71.12	3085
HSA-MIR-548X-5P	99.78	71.12	3085
HSA-MIR-3680-3P	99.75	72.51	3095
HSA-MIR-6885-3P	99.75	70.36	3187
HSA-MIR-466	99.67	70.85	2863
HSA-MIR-4804-3P	99.65	67.78	866
HSA-MIR-8061	99.63	69.44	1411
HSA-MIR-885-5P	99.59	68.59	879
HSA-MIR-4276	99.56	67.66	2514
HSA-MIR-451B	99.55	68.28	1380

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 10.2% of screened cell lines.

Literature-anchored findings (GeneRIF, showing 15)

• differences in actin binding by human ADF and cofilin (PMID:11812157)
• Important sequence differences between actin-depolymerizing factor/cofilin were correlated with unique structural determinants in the F-actin-binding site to account for differences in biochemical activities of the two proteins. (PMID:14627701)
• downregulated following UV exposure in epidermis (PMID:15816841)
• In the absence of any crystal structures of ADF or cofilin in complex with actin, these studies provide further information about the binding sites on F-actin for these important actin regulatory proteins. (PMID:17196218)
• destrin is a significant regulator of various processes important for invasive phenotype of human colon cancer Isreco1 cells whereas cofilin-1 may be involved in only a subset of them (PMID:17583572)
• The results of this study suggested that temporally regulated ADF/cofilin activities function in postsynaptic modifications of receptor number and spine size during synaptic plasticity. (PMID:20835250)
• Changes in the expression of cytoskeletal regulatory proteins such as LIMK and cofilin may play a role in weakening thoracic aortic medial tissue, as a precondition to thoracic aortic dissection. (PMID:20873970)
• The ADF/cofilin1-dependent severing of actin filaments exposes and promotes the activation of SPCA1, which pumps Ca(2+) into the lumen of the TGN for the sorting of the class of secretory cargo that binds Ca(2+). (PMID:21571222)
• Destrin is upregulated in nerve-invasive pancreatic cancer cells and its expression might be related to perineural invasiveness (PMID:22898637)
• analysis of human Cof1, Cof2, and ADF effects on actin filament severing and turnover (PMID:26996939)
• Destrin Contributes to Lung Adenocarcinoma Progression by Activating Wnt/beta-Catenin Signaling Pathway. (PMID:32878967)
• Positive natural selection of N6-methyladenosine on the RNAs of processed pseudogenes. (PMID:34120636)
• ADF and cofilin-1 collaborate to promote cortical actin flow and the leader bleb-based migration of confined cells. (PMID:34169836)
• The actin depolymerizing factor destrin serves as a negative feedback inhibitor of smooth muscle cell differentiation. (PMID:34559579)
• DSTN Hypomethylation Promotes Radiotherapy Resistance of Rectal Cancer by Activating the Wnt/beta-Catenin Signaling Pathway. (PMID:37019366)

Cross-species orthologs

7 orthologs

Organism	Symbol	Gene ID
mus_musculus	Dstn	ENSMUSG00000015932
rattus_norvegicus	Dstn	ENSRNOG00000005924
rattus_norvegicus		ENSRNOG00000073635
drosophila_melanogaster	tsr	FBGN0011726
drosophila_melanogaster	CG6873	FBGN0030951
caenorhabditis_elegans		WBGENE00006794
caenorhabditis_elegans		WBGENE00302980

Paralogs (2): CFL2 (ENSG00000165410), CFL1 (ENSG00000172757)

Protein

Protein identifiers

Destrin — P60981 (reviewed: P60981)

Alternative names: Actin-depolymerizing factor

All UniProt accessions (3): P60981, F6RFD5, V9HWA6

UniProt curated annotations — full annotation on UniProt →

Function. Actin-depolymerizing protein. Severs actin filaments (F-actin) and binds to actin monomers (G-actin). Acts in a pH-independent manner.

Tissue specificity. Widely distributed in various tissues.

Post-translational modifications. ISGylated.

Similarity. Belongs to the actin-binding proteins ADF family.

Isoforms (2)

UniProt ID	Names	Canonical?
P60981-1	1	yes
P60981-2	2

RefSeq proteins (2): NP_001011546, NP_006861* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR002108	ADF-H	Domain
IPR017904	ADF/Cofilin	Family
IPR029006	ADF-H/Gelsolin-like_dom_sf	Homologous_superfamily

Pfam: PF00241

UniProt features (9 total): modified residue 3, initiator methionine 1, chain 1, domain 1, short sequence motif 1, splice variant 1, sequence variant 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-P60981-F1	87.03	0.28

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (3): 2, 3, 19

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 267 (showing top): FREAC2_01, GRUETZMANN_PANCREATIC_CANCER_DN, AREB6_03, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_UP, HSIAO_HOUSEKEEPING_GENES, GOBP_POSITIVE_REGULATION_OF_PROTEIN_CONTAINING_COMPLEX_DISASSEMBLY, GOBP_POSITIVE_REGULATION_OF_ORGANELLE_ORGANIZATION, GOLDRATH_ANTIGEN_RESPONSE, SRF_Q5_01, GOBP_REGULATION_OF_ACTIN_FILAMENT_BASED_PROCESS, SENESE_HDAC1_AND_HDAC2_TARGETS_DN, GOBP_REGULATION_OF_ANATOMICAL_STRUCTURE_SIZE, PAPASPYRIDONOS_UNSTABLE_ATEROSCLEROTIC_PLAQUE_DN, SRF_C, LI_WILMS_TUMOR_VS_FETAL_KIDNEY_1_UP

GO Biological Process (6): actin polymerization or depolymerization (GO:0008154), actin filament depolymerization (GO:0030042), actin filament fragmentation (GO:0030043), positive regulation of actin filament depolymerization (GO:0030836), cell motility (GO:0048870), actin filament severing (GO:0051014)

GO Molecular Function (3): actin filament binding (GO:0051015), actin binding (GO:0003779), protein binding (GO:0005515)

GO Cellular Component (7): cytoplasm (GO:0005737), actin cytoskeleton (GO:0015629), cortical actin cytoskeleton (GO:0030864), extracellular exosome (GO:0070062), presynapse (GO:0098793), postsynapse (GO:0098794), glutamatergic synapse (GO:0098978)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	3
synapse	3
actin filament depolymerization	2
actin filament organization	1
actin polymerization or depolymerization	1
protein depolymerization	1
regulation of actin filament depolymerization	1
positive regulation of cytoskeleton organization	1
positive regulation of protein depolymerization	1
positive regulation of supramolecular fiber organization	1
cellular process	1
actin filament-based process	1
actin binding	1
protein-containing complex binding	1
cytoskeletal protein binding	1
binding	1
intracellular anatomical structure	1
cytoskeleton	1
actin cytoskeleton	1
cortical cytoskeleton	1
extracellular vesicle	1

Protein interactions and networks

STRING

0 interactions, top by confidence (×1000):

IntAct

105 interactions, top by confidence:

A	B	Type	Score
DSTN	ACTB	psi-mi:“MI:0915”(physical association)	0.900
ACTB	DSTN	psi-mi:“MI:0915”(physical association)	0.900
AKR7A3	AKR7A2	psi-mi:“MI:0914”(association)	0.890
DSTN	ACTG1	psi-mi:“MI:0915”(physical association)	0.790
ACTG1	DSTN	psi-mi:“MI:0915”(physical association)	0.790
CFTR	DSTN	psi-mi:“MI:0915”(physical association)	0.740
TAX1BP1	DSTN	psi-mi:“MI:0915”(physical association)	0.720
DSTN	TAX1BP1	psi-mi:“MI:0915”(physical association)	0.720
CFTR	ESYT2	psi-mi:“MI:0914”(association)	0.710
CFL1	CAP2	psi-mi:“MI:0914”(association)	0.640
CFTR	HAX1	psi-mi:“MI:0914”(association)	0.610
DSTN	PS1TP5BP1	psi-mi:“MI:0915”(physical association)	0.560

BioGRID (244): DSTN (Two-hybrid), DSTN (Two-hybrid), DSTN (Two-hybrid), DSTN (Two-hybrid), DSTN (Affinity Capture-MS), DSTN (Two-hybrid), DSTN (Two-hybrid), ABAT (Co-fractionation), ALDH4A1 (Co-fractionation), DSTN (Co-fractionation), DSTN (Co-fractionation), DSTN (Co-fractionation), DSTN (Co-fractionation), DSTN (Co-fractionation), DSTN (Co-fractionation)

ESM2 similar proteins: O60234, O88600, O95757, P10668, P18359, P18760, P21566, P23514, P23528, P34932, P45591, P45592, P45593, P45594, P45695, P48722, P54577, P60981, P60982, P60983, P60984, Q03048, Q148F1, Q2TFN9, Q4I963, Q4KM49, Q4R5C0, Q56JZ9, Q5E9D5, Q5E9F7, Q5G6V9, Q5R6P6, Q5R8T5, Q5RDM4, Q5U4Y2, Q5XHH8, Q5ZJ08, Q61316, Q63228, Q6B7M7

Diamond homologs: C4LVG4, O49606, P0CM06, P0CM07, P0DJ26, P0DJ27, P10668, P18359, P18760, P23528, P30174, P30175, P37167, P45592, P45594, P46251, P60981, P60982, P78929, P86293, Q03048, Q0D744, Q0DLA3, Q17A58, Q2QLT8, Q337A5, Q39250, Q39251, Q41764, Q43694, Q4I963, Q4P6E9, Q4R5C0, Q54R65, Q570Y6, Q5E9D5, Q5E9F7, Q5I082, Q5ZM35, Q640W2

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
TESK2	“down-regulates activity”	DSTN	phosphorylation

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 77 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Signaling by VEGF	5	22.0×	1e-03
Regulation of actin dynamics for phagocytic cup formation	5	18.4×	1e-03
Leishmania infection	5	16.3×	1e-03
Parasitic Infection Pathways	5	16.3×	1e-03
Translocation of SLC2A4 (GLUT4) to the plasma membrane	5	15.4×	1e-03
VEGFA-VEGFR2 Pathway	5	13.9×	1e-03
MAPK family signaling cascades	5	10.3×	3e-03
Diseases of signal transduction by growth factor receptors and second messengers	7	8.0×	1e-03

GO biological processes:

GO term	Partners	Fold	FDR
Ras protein signal transduction	5	15.3×	3e-03
transforming growth factor beta receptor signaling pathway	6	14.2×	2e-03
axonogenesis	5	12.0×	6e-03
positive regulation of cell migration	8	7.4×	3e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

17 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	12
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1007 predictions. Top by Δscore:

Variant	Effect	Δscore
20:17601043:GTG:G	donor_gain	1.0000
20:17601045:GGTA:G	donor_loss	1.0000
20:17601046:GT:G	donor_loss	1.0000
20:17570208:GATG:G	donor_gain	0.9900
20:17570209:ATGG:A	donor_loss	0.9900
20:17570210:TG:T	donor_gain	0.9900
20:17570210:TGGT:T	donor_loss	0.9900
20:17570211:GG:G	donor_gain	0.9900
20:17570212:G:GG	donor_gain	0.9900
20:17570212:G:T	donor_loss	0.9900
20:17570213:TGA:T	donor_loss	0.9900
20:17570214:GAG:G	donor_loss	0.9900
20:17590477:ATGGG:A	acceptor_gain	0.9900
20:17600733:CATA:C	acceptor_loss	0.9900
20:17600735:TAGGC:T	acceptor_loss	0.9900
20:17600736:A:AG	acceptor_gain	0.9900
20:17600736:A:G	acceptor_loss	0.9900
20:17600737:G:GG	acceptor_gain	0.9900
20:17600737:G:GT	acceptor_loss	0.9900
20:17601023:G:GT	donor_gain	0.9900
20:17601046:G:GG	donor_gain	0.9900
20:17601047:T:G	donor_loss	0.9900
20:17601048:AA:A	donor_loss	0.9900
20:17604552:TAGG:T	acceptor_loss	0.9900
20:17604553:A:AG	acceptor_gain	0.9900
20:17604554:G:GA	acceptor_loss	0.9900
20:17604554:G:GG	acceptor_gain	0.9900
20:17604542:T:G	acceptor_gain	0.9800
20:17604553:AG:A	acceptor_gain	0.9800
20:17604554:GG:G	acceptor_gain	0.9800

AlphaMissense

1096 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
20:17600744:G:A	G4R	1.000
20:17600744:G:C	G4R	1.000
20:17600745:G:A	G4E	1.000
20:17600978:T:C	Y82H	1.000
20:17600999:T:C	F89L	1.000
20:17601001:T:A	F89L	1.000
20:17601001:T:G	F89L	1.000
20:17601044:T:A	W104R	1.000
20:17601044:T:C	W104R	1.000
20:17604585:A:C	K114N	1.000
20:17604585:A:T	K114N	1.000
20:17604587:T:A	M115K	1.000
20:17604587:T:C	M115T	1.000
20:17604587:T:G	M115R	1.000
20:17604602:C:T	S120F	1.000
20:17600745:G:T	G4V	0.999
20:17600777:T:C	F15L	0.999
20:17600779:T:A	F15L	0.999
20:17600779:T:G	F15L	0.999
20:17600791:A:C	K19N	0.999
20:17600791:A:T	K19N	0.999
20:17600830:A:C	R32S	0.999
20:17600830:A:T	R32S	0.999
20:17600836:G:C	K34N	0.999
20:17600836:G:T	K34N	0.999
20:17600841:T:A	V36D	0.999
20:17600847:T:C	F38S	0.999
20:17600874:T:A	I47N	0.999
20:17600874:T:C	I47T	0.999
20:17600874:T:G	I47S	0.999

dbSNP variants (sampled 300 via entrez): RS1000011717 (20:17605741 A>G), RS1000110313 (20:17579970 T>A), RS1000141934 (20:17589147 C>A,T), RS1000269101 (20:17586238 C>G), RS1000420569 (20:17593186 G>C), RS1000497831 (20:17609931 CTG>C), RS1000523595 (20:17600153 T>C), RS1000611030 (20:17573407 G>A,T), RS1000700560 (20:17606623 G>A), RS1000808552 (20:17585933 G>A), RS1000809018 (20:17568960 G>A), RS1000815338 (20:17606415 G>A), RS1000825712 (20:17579389 A>G), RS1000943079 (20:17568686 C>A,G,T), RS1001112318 (20:17581392 G>A)

Disease associations

OMIM: gene MIM:609114 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

2 associations (top):

Study	Trait	p-value
GCST001872_12	Presence of antiphospholipid antibodies	6.000000e-06
GCST005196_242	Coronary artery disease	1.000000e-06

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL5725005 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
5.76	Kd	1744	nM	CHEMBL5653589
5.76	ED50	1744	nM	CHEMBL5653589

PubChem BioAssay actives

1 with measured affinity, of 8 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2148266: Binding affinity to human DSTN incubated for 45 mins by Kinobead based pull down assay	kd	1.7440	uM

CTD chemical–gene interactions

53 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Valproic Acid	affects cotreatment, increases expression, decreases expression	4
sodium arsenite	decreases expression, affects cotreatment	3
Smoke	increases expression, decreases expression	2
Tetrachlorodibenzodioxin	increases expression	2
aristolochic acid I	decreases expression	1
FR900359	increases phosphorylation	1
TAK-243	increases sumoylation	1
methylmercuric chloride	decreases expression	1
triphenyl phosphate	affects expression	1
bisphenol A	increases expression	1
lead acetate	affects cotreatment, decreases expression	1
sodium arsenate	decreases expression	1
pyrogallol 1,3-dimethyl ether	decreases expression, increases expression, affects cotreatment, affects localization	1
kojic acid	increases expression	1
arsenite	increases reaction, affects binding	1
nickel sulfate	decreases expression	1
N,N,N’,N’-tetrakis(2-pyridylmethyl)ethylenediamine	increases expression	1
perfluorooctane sulfonic acid	decreases expression	1
CGP 52608	affects binding, increases reaction	1
chloropicrin	increases expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, increases expression	1
nutlin 3	affects cotreatment, increases secretion	1
ICG 001	decreases expression	1
bromovanin	decreases expression	1
dorsomorphin	affects cotreatment, increases expression	1
bisphenol S	increases expression	1
jinfukang	decreases expression	1
bisphenol AF	increases expression	1
Resveratrol	affects expression	1
Sunitinib	increases expression	1

ChEMBL screening assays

7 unique, capped per target: 7 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5651308	Binding	Binding affinity to human DSTN incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.