DUSP23

Summary

DUSP23 (dual specificity phosphatase 23, HGNC:21480) is a protein-coding gene on chromosome 1q23.2, encoding Dual specificity protein phosphatase 23 (Q9BVJ7). Protein phosphatase that mediates dephosphorylation of proteins phosphorylated on Tyr and Ser/Thr residues.

Enables protein tyrosine/serine/threonine phosphatase activity. Involved in dephosphorylation. Located in nucleoplasm.

Source: NCBI Gene 54935 — RefSeq curated summary.

At a glance

• GWAS associations: 5
• Clinical variants (ClinVar): 39 total
• Druggable target: yes
• MANE Select transcript: NM_001319658

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:21480
Approved symbol	DUSP23
Name	dual specificity phosphatase 23
Location	1q23.2
Locus type	gene with protein product
Status	Approved
Aliases	FLJ20442, DUSP25
Ensembl gene	ENSG00000158716
Ensembl biotype	protein_coding
OMIM	618361
Entrez	54935

Gene structure

Transcript identifiers

Ensembl transcripts: 5 — 5 protein_coding

ENST00000368107, ENST00000368108, ENST00000368109, ENST00000880030, ENST00000912050

RefSeq mRNA: 3 — MANE Select: NM_001319658 NM_001319658, NM_001319659, NM_017823

CCDS: CCDS1187

Canonical transcript exons

ENST00000368107 — 2 exons

Exon	Start	End
ENSE00001446336	159780962	159781367
ENSE00001446338	159782153	159782543

Expression profiles

Bgee: expression breadth ubiquitous, 240 present calls, max score 97.08.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 14.7701 / max 180.9927, expressed in 1693 samples.

FANTOM5 promoters (6 alternative TSS)

Promoter ID	TPM avg	Samples expressed
5984	7.4877	1662
5985	5.9481	1481
5988	0.8109	475
5986	0.3562	159
5989	0.0947	36
5987	0.0724	25

Top tissues by expression

249 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
left adrenal gland cortex	UBERON:0035825	97.08	gold quality
left adrenal gland	UBERON:0001234	96.99	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	96.94	gold quality
right lobe of liver	UBERON:0001114	96.81	gold quality
right adrenal gland	UBERON:0001233	96.76	gold quality
minor salivary gland	UBERON:0001830	96.67	gold quality
right adrenal gland cortex	UBERON:0035827	96.49	gold quality
adrenal cortex	UBERON:0001235	96.33	gold quality
mucosa of transverse colon	UBERON:0004991	96.21	gold quality
mouth mucosa	UBERON:0003729	96.03	gold quality
adult mammalian kidney	UBERON:0000082	95.67	gold quality
omental fat pad	UBERON:0010414	94.86	gold quality
peritoneum	UBERON:0002358	94.82	gold quality
metanephros cortex	UBERON:0010533	94.82	gold quality
saliva-secreting gland	UBERON:0001044	94.73	gold quality
esophagus mucosa	UBERON:0002469	94.71	gold quality
tendon of biceps brachii	UBERON:0008188	94.64	gold quality
left uterine tube	UBERON:0001303	94.60	gold quality
adipose tissue of abdominal region	UBERON:0007808	94.31	gold quality
adrenal gland	UBERON:0002369	94.25	gold quality
right lobe of thyroid gland	UBERON:0001119	94.10	gold quality
gingival epithelium	UBERON:0001949	93.94	gold quality
skin of abdomen	UBERON:0001416	93.73	gold quality
left coronary artery	UBERON:0001626	93.69	gold quality
endocervix	UBERON:0000458	93.68	gold quality
gingiva	UBERON:0001828	93.50	gold quality
left lobe of thyroid gland	UBERON:0001120	93.43	gold quality
cortex of kidney	UBERON:0001225	93.39	gold quality
lower esophagus mucosa	UBERON:0035834	93.37	gold quality
granulocyte	CL:0000094	93.28	gold quality

Single-cell (SCXA)

Detected in 10 experiment(s), a significant marker in 10.

Experiment	Marker?	Max mean expression
E-GEOD-124472	yes	1872.73
E-GEOD-135922	yes	285.07
E-MTAB-10287	yes	74.39
E-HCAD-1	yes	34.88
E-MTAB-6701	yes	30.06
E-CURD-114	yes	27.09
E-MTAB-8410	yes	24.96
E-HCAD-6	yes	20.18
E-HCAD-13	yes	7.30
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

17 targeting DUSP23, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3065-5P	99.97	71.56	3281
HSA-MIR-4725-3P	99.96	69.53	2520
HSA-MIR-6780B-5P	99.96	69.60	2562
HSA-MIR-4729	99.69	72.18	4233
HSA-MIR-6733-3P	99.54	67.80	1281
HSA-MIR-7159-5P	99.53	72.12	2472
HSA-MIR-3127-3P	98.94	67.34	1055
HSA-MIR-6756-3P	98.94	66.79	1104
HSA-MIR-3154	98.94	66.55	1455
HSA-MIR-629-5P	98.78	68.72	1032
HSA-MIR-4272	98.76	68.74	1810
HSA-MIR-6827-5P	98.46	64.88	1256
HSA-MIR-3157-5P	97.41	67.61	998
HSA-MIR-6726-5P	95.97	63.72	841
HSA-MIR-920	95.97	63.95	811
HSA-MIR-4300	95.85	64.56	1003
HSA-MIR-5591-5P	95.85	64.76	1002

Literature-anchored findings (GeneRIF, showing 4)

• DUSP23 showed distinctive phosphatase activity toward p-nitrophenyl phosphate, as well as oligopeptides containing phospho-tyrosine and phospho-threonine residues. Furthermore, DUSP23 could dephosphorylate p44ERK1 but not p38 and p54SAPKbeta in vitro (PMID:15147733)
• the smallest active protein-tyrosine phosphatase yet (only 16 kDa) belongs to the group of small Vaccinia virus VH1-related dual specific phosphatases; gene is located on human chromosome 1q23.1 and consists of only two coding exons (PMID:15201283)
• The upregulation of VHZ levels in primary human cancers supports the clinical relevance of this protein in cancers (PMID:20509867)
• this study shows that DUSP23 mRNA levels in CD4+ T cells are higher in the systemic lupus erythematosus patients (PMID:27737517)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	dusp23a	ENSDARG00000009844
danio_rerio	dusp23b	ENSDARG00000089245
mus_musculus	Dusp23	ENSMUSG00000026544
rattus_norvegicus	Dusp23	ENSRNOG00000022143

Protein

Protein identifiers

Dual specificity protein phosphatase 23 — Q9BVJ7 (reviewed: Q9BVJ7)

Alternative names: Low molecular mass dual specificity phosphatase 3, VH1-like phosphatase Z

All UniProt accessions (2): A0A140VJI5, Q9BVJ7

UniProt curated annotations — full annotation on UniProt →

Function. Protein phosphatase that mediates dephosphorylation of proteins phosphorylated on Tyr and Ser/Thr residues. In vitro, it can dephosphorylate p44-ERK1 (MAPK3) but not p54 SAPK-beta (MAPK10) in vitro. Able to enhance activation of JNK and p38 (MAPK14).

Subcellular location. Cytoplasm. Cytosol. Nucleus.

Tissue specificity. Widely expressed. Highly expressed in spleen, prostate, colon, adrenal gland, mammary gland, thyroid and trachea. Expressed at lower level in uterus, small intestine, bladder, bone marrow, brain, spinal cord and stomach.

Similarity. Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

RefSeq proteins (3): NP_001306587, NP_001306588, NP_060293 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR000387	Tyr_Pase_dom	Domain
IPR003595	Tyr_Pase_cat	Domain
IPR016130	Tyr_Pase_AS	Active_site
IPR020422	TYR_PHOSPHATASE_DUAL_dom	Domain
IPR029021	Prot-tyrosine_phosphatase-like	Homologous_superfamily
IPR050561	PTP	Family
IPR057023	PTP-SAK	Domain

Pfam: PF22784

Enzyme classification (BRENDA):

• EC 3.1.3.48 — protein-tyrosine-phosphatase (BRENDA: 59 organisms, 501 substrates, 1326 inhibitors, 270 Km, 165 kcat entries)

Substrate kinetics (BRENDA)

70 substrates with measured Km, best-characterized 15. Km ranges are aggregated across organisms/conditions.

Substrate	Km (mM)	Measurements
4-NITROPHENYL PHOSPHATE	0.0008–148	84
6,8-DIFLUORO-4-METHYLUMBELLIFERYL PHOSPHATE	0.0039–0.862	27
P-NITROPHENYL PHOSPHATE	0.0024–10	20
DADEPYLIPQQG	0.0003–0.1	12
PHOSPHOTYROSINE	0.012–30	11
LYSOZYME	0.0003–0.012	5
MYELIN BASIC PROTEIN	0.0001–0.022	5
ACETYL-DADEPY-NH2	0.0228–0.219	4
ACETYL-DADEPYL-NH2	1.1–97.5	4
4,6,8-TRIMETHYL-2-OXO-2H-CHROMEN-7-YL DIHYDROGEN	0.02–0.156	3
SASASPYSASA	0.53–2.3	3
1-NAPHTHYL PHOSPHATE	1.19–1.88	2
3,6-FLUORESCEIN DIPHOSPHATE	15–19	2
4-METHYLUMBELLIFERYL PHOSPHATE	0.953–2.41	2
BOVINE SERUM ALBUMIN	0.0001–0.0003	2

Catalyzed reactions (Rhea), 3 shown:

• O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate (RHEA:10684)
• O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate (RHEA:20629)
• O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate (RHEA:47004)

UniProt features (21 total): strand 7, helix 6, sequence variant 2, initiator methionine 1, chain 1, domain 1, active site 1, sequence conflict 1, turn 1

Structure

Experimental structures (PDB)

2 structures.

PDB	Method	Resolution (Å)
4ERC	X-RAY DIFFRACTION	1.15
2IMG	X-RAY DIFFRACTION	1.93

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9BVJ7-F1	97.55	0.98

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Catalytic / active sites (1): 95 (phosphocysteine intermediate)

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 81 (showing top): CREIGHTON_ENDOCRINE_THERAPY_RESISTANCE_1, WANG_LMO4_TARGETS_DN, GOBP_CILIUM_ORGANIZATION, GOBP_ORGANELLE_ASSEMBLY, GOBP_DEPHOSPHORYLATION, GOBP_CELL_PROJECTION_ORGANIZATION, ACEVEDO_LIVER_CANCER_UP, GOMF_HYDROLASE_ACTIVITY_ACTING_ON_ESTER_BONDS, GOMF_PROTEIN_SERINE_THREONINE_PHOSPHATASE_ACTIVITY, GOMF_PHOSPHORIC_ESTER_HYDROLASE_ACTIVITY, GOMF_PROTEIN_TYROSINE_SERINE_THREONINE_PHOSPHATASE_ACTIVITY, GOMF_PROTEIN_TYROSINE_PHOSPHATASE_ACTIVITY, GSE13762_CTRL_VS_125_VITAMIND_DAY12_DC_UP, CHEN_LIVER_METABOLISM_QTL_CIS, HORIUCHI_WTAP_TARGETS_UP

GO Biological Process (3): dephosphorylation (GO:0016311), cilium assembly (GO:0060271), protein dephosphorylation (GO:0006470)

GO Molecular Function (7): protein serine/threonine phosphatase activity (GO:0004722), protein tyrosine phosphatase activity (GO:0004725), protein tyrosine/serine/threonine phosphatase activity (GO:0008138), phosphatase activity (GO:0016791), phosphoprotein phosphatase activity (GO:0004721), protein binding (GO:0005515), hydrolase activity (GO:0016787)

GO Cellular Component (4): nucleoplasm (GO:0005654), cytoplasm (GO:0005737), cytosol (GO:0005829), nucleus (GO:0005634)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
phosphoprotein phosphatase activity	3
cellular anatomical structure	3
phosphate-containing compound metabolic process	1
axoneme assembly	1
intraciliary transport involved in cilium assembly	1
cilium organization	1
protein localization to cilium	1
organelle assembly	1
trans-Golgi to periciliary membrane compartment transport	1
plasma membrane bounded cell projection assembly	1
ciliary transition zone assembly	1
dephosphorylation	1
protein modification process	1
phosphoric ester hydrolase activity	1
phosphatase activity	1
catalytic activity, acting on a protein	1
binding	1
catalytic activity	1
nuclear lumen	1
intracellular anatomical structure	1
cytoplasm	1
intracellular membrane-bounded organelle	1

Protein interactions and networks

STRING

1030 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
DUSP23	DUSP15	Q9H1R2	728
DUSP23	DUSP3	P51452	671
DUSP23	DUSP22	Q9NRW4	584
DUSP23	PTS	Q03393	531
DUSP23	EPM2A	O95278	512
DUSP23	PTPN7	P35236	488
DUSP23	ACP1	P24666	476
DUSP23	DUSP1	P28562	475
DUSP23	ITGB4	P16144	459
DUSP23	PTPRA	P18433	456
DUSP23	DUSP29	Q68J44	451
DUSP23	DUSP19	Q8WTR2	438
DUSP23	DUSP10	Q9Y6W6	437
DUSP23	PTPN3	P26045	431
DUSP23	DUSP5	Q16690	430

IntAct

77 interactions, top by confidence:

A	B	Type	Score
EXOSC8	DUSP23	psi-mi:“MI:0915”(physical association)	0.810
DUSP23	EXOSC8	psi-mi:“MI:0915”(physical association)	0.810
DUSP23	NIF3L1	psi-mi:“MI:0915”(physical association)	0.560
NIF3L1	DUSP23	psi-mi:“MI:0915”(physical association)	0.560
UBA3	DUSP23	psi-mi:“MI:0915”(physical association)	0.560
DUSP23	BANF2	psi-mi:“MI:0915”(physical association)	0.560
TCF4	DUSP23	psi-mi:“MI:0915”(physical association)	0.560
DDIT4L	DUSP23	psi-mi:“MI:0915”(physical association)	0.560
PSMB8	DUSP23	psi-mi:“MI:0915”(physical association)	0.560
DUSP23	PSME2	psi-mi:“MI:0915”(physical association)	0.560
DUSP23	ASB6	psi-mi:“MI:0915”(physical association)	0.560
MRNIP	DUSP23	psi-mi:“MI:0915”(physical association)	0.560
DUSP23	POTEI	psi-mi:“MI:0915”(physical association)	0.400
DUSP23	PLK4	psi-mi:“MI:0915”(physical association)	0.400
ASB13	DUSP23	psi-mi:“MI:0915”(physical association)	0.370
DUSP23	CCDC186	psi-mi:“MI:0915”(physical association)	0.370
EDRF1	DUSP23	psi-mi:“MI:0915”(physical association)	0.370
DUSP23	DBI	psi-mi:“MI:0915”(physical association)	0.370
DBN1	DUSP23	psi-mi:“MI:0915”(physical association)	0.370
DUSP23	DNMT3B	psi-mi:“MI:0915”(physical association)	0.370
DUSP23	EED	psi-mi:“MI:0915”(physical association)	0.370
KAT5	DUSP23	psi-mi:“MI:0915”(physical association)	0.370
DUSP23	KBTBD7	psi-mi:“MI:0915”(physical association)	0.370

BioGRID (130): DUSP23 (Two-hybrid), NIF3L1 (Two-hybrid), EXOSC8 (Two-hybrid), PLK4 (Affinity Capture-MS), RALGAPA1 (Proximity Label-MS), DUSP23 (Affinity Capture-MS), DUSP23 (Affinity Capture-MS), DUSP23 (Affinity Capture-MS), RAD50 (Affinity Capture-MS), TMEM70 (Affinity Capture-MS), RAC1 (Affinity Capture-MS), RAP1GDS1 (Affinity Capture-MS), PGRMC2 (Affinity Capture-MS), AP3S1 (Affinity Capture-MS), VDAC3 (Affinity Capture-MS)

ESM2 similar proteins: A2VDS1, A4IHU7, A5GFY8, O95147, O95571, P0C089, P0C591, P0C595, P0C596, P0C5A1, P23919, P51452, P53607, Q0B569, Q0VCI1, Q17QM8, Q3T094, Q4KL92, Q4P7L6, Q5BJY6, Q5F389, Q5R9W5, Q5RCH4, Q5RD73, Q5ZJH6, Q5ZKP6, Q62951, Q66GT5, Q6AXW7, Q6JQN1, Q6NT99, Q6PHR2, Q8BK84, Q8BWU5, Q8JZV7, Q8VE01, Q91348, Q91WL8, Q94529, Q99N11

Diamond homologs: A0A0R4IVA4, A1L1R5, A2A3K4, A4D256, A6N3Q4, A7E379, O60729, P35821, P81299, Q00684, Q196Z3, Q4CUJ8, Q59NH8, Q63739, Q6GQT0, Q6NT99, Q6NZK8, Q78EG7, Q86IL4, Q93096, Q9BVJ7, Q9TSM6, Q9UNH5, Q6NKR2, Q9ZQP1, O10274, O55236, O60942, O75319, P24656, P34442, Q17607, Q22707, Q4KM79, Q5E999, Q6NXK5, Q6NY98, Q9P7H1, P70261, Q6DIR8

SIGNOR signaling

2 interactions.

A	Effect	B	Mechanism
DUSP23	“up-regulates quantity by stabilization”	GCM1	dephosphorylation
DUSP23	“down-regulates activity”	MAPK3	dephosphorylation

Disease & clinical

Clinical variants and AI predictions

ClinVar

39 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	35
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

486 predictions. Top by Δscore:

Variant	Effect	Δscore
1:159780992:AGG:A	donor_loss	0.9900
1:159780993:GG:G	donor_loss	0.9900
1:159780994:G:T	donor_loss	0.9900
1:159780995:T:G	donor_loss	0.9900
1:159781362:G:GT	donor_gain	0.9900
1:159781362:G:T	donor_gain	0.9900
1:159781364:AGAG:A	donor_loss	0.9900
1:159781365:GAG:G	donor_gain	0.9900
1:159781366:AG:A	donor_loss	0.9900
1:159781367:GG:G	donor_loss	0.9900
1:159781097:A:AG	acceptor_gain	0.9800
1:159781097:AGC:A	acceptor_gain	0.9800
1:159781098:G:GG	acceptor_gain	0.9800
1:159781098:GC:G	acceptor_gain	0.9800
1:159781098:GCG:G	acceptor_gain	0.9800
1:159781099:C:CA	acceptor_gain	0.9800
1:159781344:G:GT	donor_gain	0.9800
1:159781364:A:T	donor_gain	0.9800
1:159781391:G:GT	donor_gain	0.9800
1:159782152:GGCT:G	acceptor_gain	0.9800
1:159781094:CCCA:C	acceptor_loss	0.9700
1:159781095:CCAGC:C	acceptor_loss	0.9700
1:159781096:CA:C	acceptor_loss	0.9700
1:159781098:G:GC	acceptor_loss	0.9700
1:159781098:GCGAT:G	acceptor_gain	0.9700
1:159781098:GCGA:G	acceptor_gain	0.9600
1:159781363:GAGAG:G	donor_gain	0.9600
1:159782147:TCGTA:T	acceptor_loss	0.9600
1:159782148:CGTA:C	acceptor_loss	0.9600
1:159782149:GTAGG:G	acceptor_loss	0.9600

AlphaMissense

942 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
1:159782178:G:A	G98D	0.993
1:159782310:T:A	V142D	0.993
1:159782170:T:G	C95W	0.992
1:159782178:G:T	G98V	0.992
1:159782168:T:C	C95R	0.991
1:159782169:G:A	C95Y	0.991
1:159782177:G:C	G98R	0.990
1:159782186:C:A	R101S	0.990
1:159782205:C:A	A107D	0.988
1:159782207:T:C	C108R	0.988
1:159782209:T:G	C108W	0.988
1:159782299:G:C	Q138H	0.988
1:159782299:G:T	Q138H	0.988
1:159782160:G:A	G92E	0.987
1:159782184:G:A	G100D	0.987
1:159781216:T:C	L39P	0.986
1:159782193:G:A	G103D	0.986
1:159782177:G:T	G98C	0.985
1:159782262:G:C	R126P	0.985
1:159781153:G:A	G18E	0.984
1:159781219:T:A	V40E	0.984
1:159782208:G:A	C108Y	0.983
1:159782159:G:A	G92R	0.982
1:159782159:G:C	G92R	0.982
1:159782184:G:T	G100V	0.982
1:159782318:T:C	F145L	0.982
1:159782320:C:A	F145L	0.982
1:159782320:C:G	F145L	0.982
1:159782190:C:T	T102I	0.981
1:159782283:T:A	I133N	0.981

dbSNP variants (sampled 300 via entrez): RS1000747334 (1:159781044 A>G), RS1000833009 (1:159780481 A>C), RS1001201071 (1:159781311 C>A), RS1002125415 (1:159781870 A>G), RS1002200377 (1:159781564 AACTG>A), RS1002884646 (1:159782015 G>C), RS1002920911 (1:159781652 G>A,C,T), RS1003165704 (1:159782972 C>A), RS1003892984 (1:159780791 C>A,G,T), RS1005083944 (1:159779882 A>G), RS1006126451 (1:159781024 G>A,T), RS1006271169 (1:159779153 G>A,C), RS1006283777 (1:159779423 G>A), RS1007122679 (1:159782389 C>A,T), RS1007922430 (1:159781276 A>T)

Disease associations

OMIM: gene MIM:618361 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

5 associations (top):

Study	Trait	p-value
GCST001136_2	Interstitial lung disease	9.000000e-06
GCST001650_1	C-reactive protein	1.000000e-37
GCST001650_11	C-reactive protein	3.000000e-10
GCST001650_8	C-reactive protein	4.000000e-73
GCST003654_2	Bone mineral density (Ward’s triangle area)	1.000000e-06

EFO canonical traits (2, from GWAS)

EFO ID	Trait name
EFO:0004458	C-reactive protein measurement
EFO:0007785	femoral neck bone mineral density

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL2396506 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

Binding affinities (BindingDB)

1 measured of 1 human assays (1 total across all organisms); most potent 1 below. Values come from heterogeneous assays and are not directly comparable.

Ligand	Measure	Value	Patent
6-hydroxy-3-iodo-1-methyl-2-[3-[[2-oxo-2-(4-thiophen-3-ylanilino)acetyl]amino]phenyl]indole-5-carboxylic acid	IC50	2900 nM	US-9522881: Hydroxyindole carboxylic acid based inhibitors for oncogenic Src homology-2 domain containing protein tyrosine phosphatase-2 (SHP2)

ChEMBL bioactivities

3 potent at pChembl≥5 of 5 total, top 3 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
5.64	IC50	2300	nM	CHEMBL3319356
5.47	IC50	3400	nM	CHEMBL2396718
5.24	IC50	5700	nM	CHEMBL2396719

PubChem BioAssay actives

3 with measured affinity, of 17 total; 3 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
6-hydroxy-3-iodo-1-methyl-2-[3-[[2-oxo-2-(4-thiophen-3-ylanilino)acetyl]amino]phenyl]indole-5-carboxylic acid	1182548: Inhibition of VHZ (unknown origin)	ic50	2.3000	uM
3-[2-(3-chlorophenyl)ethynyl]-6-hydroxy-2-[4-[2-oxo-2-(propylamino)ethoxy]phenyl]-1-benzofuran-5-carboxylic acid	755760: Inhibition of recombinant VHZ (unknown origin) using pNPP as substrate by spectrophotometric analysis	ic50	3.4000	uM
3-[2-(3-chlorophenyl)ethynyl]-2-[4-[2-(cyclopropylamino)-2-oxoethoxy]phenyl]-6-hydroxy-1-benzofuran-5-carboxylic acid	755760: Inhibition of recombinant VHZ (unknown origin) using pNPP as substrate by spectrophotometric analysis	ic50	5.7000	uM

CTD chemical–gene interactions

38 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Valproic Acid	affects cotreatment, increases expression, affects expression	6
Air Pollutants	decreases expression, increases abundance	2
Cisplatin	affects cotreatment, affects expression, decreases expression	2
Smoke	decreases expression, increases abundance	2
Tobacco Smoke Pollution	decreases expression	2
GSK-J4	decreases expression	1
triphenyl phosphate	affects expression	1
bisphenol A	decreases expression	1
lead acetate	decreases expression	1
nitrophenylphosphate	decreases phosphorylation, decreases reaction	1
methylparaben	decreases expression	1
sodium arsenite	decreases expression	1
cobaltous chloride	decreases expression	1
zinc chromate	decreases expression, increases abundance	1
nickel sulfate	decreases expression	1
di-n-butylphosphoric acid	affects expression	1
chromium hexavalent ion	decreases expression, increases abundance	1
CGP 52608	affects binding, increases reaction	1
entinostat	increases expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, increases expression	1
nutlin 3	increases secretion, affects cotreatment	1
dorsomorphin	affects cotreatment, increases expression	1
Temozolomide	decreases expression	1
Sunitinib	decreases expression	1
Panobinostat	affects cotreatment, affects expression	1
Benzo(a)pyrene	decreases expression	1
Dactinomycin	increases secretion, affects cotreatment	1
Edetic Acid	decreases phosphorylation, decreases reaction	1
Ethylmaleimide	decreases phosphorylation, decreases reaction	1
Phthalic Acids	increases methylation	1

ChEMBL screening assays

6 unique, capped per target: 6 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL2401182	Binding	Inhibition of recombinant VHZ (unknown origin) using pNPP as substrate by spectrophotometric analysis	A potent and selective small-molecule inhibitor for the lymphoid-specific tyrosine phosphatase (LYP), a target associated with autoimmune diseases. — J Med Chem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.