DYNLL2

Summary

DYNLL2 (dynein light chain LC8-type 2, HGNC:24596) is a protein-coding gene on chromosome 17q22, encoding Dynein light chain 2, cytoplasmic (Q96FJ2). Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function.

Predicted to enable dynein intermediate chain binding activity. Predicted to be involved in microtubule-based process. Located in 9+0 non-motile cilium and centrosome. Is active in glutamatergic synapse and postsynapse.

Source: NCBI Gene 140735 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 7 total
• Druggable target: yes
• MANE Select transcript: NM_080677

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:24596
Approved symbol	DYNLL2
Name	dynein light chain LC8-type 2
Location	17q22
Locus type	gene with protein product
Status	Approved
Aliases	MGC17810, Dlc2, DNCL1B, RSPH22
Ensembl gene	ENSG00000264364
Ensembl biotype	protein_coding
OMIM	608942
Entrez	140735

Gene structure

Transcript identifiers

Ensembl transcripts: 6 — 6 protein_coding

ENST00000579991, ENST00000908491, ENST00000908492, ENST00000908493, ENST00000931536, ENST00000945285

RefSeq mRNA: 1 — MANE Select: NM_080677 NM_080677

CCDS: CCDS11601

Canonical transcript exons

ENST00000579991 — 3 exons

Exon	Start	End
ENSE00000820250	58087082	58087222
ENSE00001291217	58083419	58083683
ENSE00002718456	58089142	58095542

Expression profiles

Bgee: expression breadth ubiquitous, 256 present calls, max score 99.19.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 68.4538 / max 598.9114, expressed in 1825 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
161893	68.4538	1825

Top tissues by expression

256 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
medial globus pallidus	UBERON:0002477	99.19	gold quality
sperm	CL:0000019	98.98	gold quality
left ventricle myocardium	UBERON:0006566	98.98	gold quality
globus pallidus	UBERON:0001875	98.82	gold quality
cardiac muscle of right atrium	UBERON:0003379	98.66	gold quality
oviduct epithelium	UBERON:0004804	98.56	gold quality
upper arm skin	UBERON:0004263	98.52	gold quality
ileal mucosa	UBERON:0000331	98.27	gold quality
pons	UBERON:0000988	98.18	gold quality
superior vestibular nucleus	UBERON:0007227	98.06	gold quality
substantia nigra pars compacta	UBERON:0001965	98.04	gold quality
ventral tegmental area	UBERON:0002691	97.95	gold quality
kidney epithelium	UBERON:0004819	97.94	gold quality
substantia nigra pars reticulata	UBERON:0001966	97.93	gold quality
medulla oblongata	UBERON:0001896	97.81	gold quality
lateral nuclear group of thalamus	UBERON:0002736	97.78	gold quality
dorsal plus ventral thalamus	UBERON:0001897	97.66	gold quality
lateral globus pallidus	UBERON:0002476	97.62	gold quality
midbrain	UBERON:0001891	97.55	gold quality
tendon of biceps brachii	UBERON:0008188	97.53	gold quality
substantia nigra	UBERON:0002038	97.50	gold quality
parietal lobe	UBERON:0001872	97.44	gold quality
myocardium	UBERON:0002349	97.42	gold quality
postcentral gyrus	UBERON:0002581	97.42	gold quality
subthalamic nucleus	UBERON:0001906	97.38	gold quality
superior frontal gyrus	UBERON:0002661	97.34	gold quality
inferior vagus X ganglion	UBERON:0005363	97.29	gold quality
occipital lobe	UBERON:0002021	97.18	gold quality
putamen	UBERON:0001874	97.17	gold quality
primary visual cortex	UBERON:0002436	97.13	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	5.18

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

95 targeting DYNLL2, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-8485	100.00	77.57	4731
HSA-MIR-4673	100.00	66.64	1490
HSA-MIR-4789-3P	99.99	70.75	2484
HSA-MIR-4789-5P	99.98	70.76	2721
HSA-MIR-27A-3P	99.98	72.13	2955
HSA-MIR-27B-3P	99.98	72.13	2955
HSA-MIR-9985	99.98	72.11	2939
HSA-MIR-4645-5P	99.98	65.81	1284
HSA-LET-7F-2-3P	99.98	70.98	2588
HSA-MIR-1185-1-3P	99.98	71.04	2593
HSA-MIR-1185-2-3P	99.98	71.04	2593
HSA-MIR-128-3P	99.95	71.17	2484
HSA-MIR-216A-3P	99.95	71.19	2505
HSA-MIR-454-3P	99.91	74.01	1925
HSA-MIR-329-3P	99.91	66.56	1234
HSA-MIR-362-3P	99.91	66.38	1267
HSA-MIR-130A-3P	99.90	73.31	1861
HSA-MIR-130B-3P	99.90	73.27	1850
HSA-MIR-301A-3P	99.90	73.15	1839
HSA-MIR-301B-3P	99.90	73.19	1836
HSA-MIR-3666	99.90	73.24	1833
HSA-MIR-4295	99.90	73.11	1838
HSA-MIR-3681-3P	99.88	70.46	2254
HSA-MIR-3941	99.86	70.54	2735
HSA-MIR-659-3P	99.85	70.69	1620
HSA-MIR-4639-5P	99.81	67.37	1028
HSA-MIR-6739-5P	99.80	67.87	2806
HSA-MIR-139-5P	99.80	69.50	1399
HSA-MIR-5002-5P	99.76	70.84	1763
HSA-MIR-623	99.76	68.16	1170

Literature-anchored findings (GeneRIF, showing 2)

• the thermodynamic and kinetic fine-tuning of binding of various ligands to DYNLL could have physiological relevance in its interaction network. (PMID:20889982)
• The DYNLL2 binding region, located in an intrinsically disordered domain of the myo5a tail, has a nascent helical character. (PMID:25312846)

Cross-species orthologs

7 orthologs

Organism	Symbol	Gene ID
danio_rerio	dynll2b	ENSDARG00000005172
danio_rerio	dynll2a	ENSDARG00000069790
mus_musculus	Dynll2	ENSMUSG00000020483
rattus_norvegicus	Dynll2	ENSRNOG00000008921
drosophila_melanogaster	ctp	FBGN0011760
drosophila_melanogaster	Cdlc2	FBGN0026141
caenorhabditis_elegans		WBGENE00001005

Paralogs (1): DYNLL1 (ENSG00000088986)

Protein

Protein identifiers

Dynein light chain 2, cytoplasmic — Q96FJ2 (reviewed: Q96FJ2)

Alternative names: 8 kDa dynein light chain b, Dynein light chain LC8-type 2

All UniProt accessions (1): Q96FJ2

UniProt curated annotations — full annotation on UniProt →

Function. Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures.

Subunit / interactions. Homodimer. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits which present intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. Dynein ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Interacts with DYNC1I1. Interacts with BMF. Component of the myosin V motor complex. Interacts with BCAS1. Interacts with Basson/BSN. Interacts with AMBRA1 (via TQT motifs); tethering AMBRA1 to the cytoskeleton. Interacts with IQUB.

Subcellular location. Cytoplasm. Cytoskeleton.

Similarity. Belongs to the dynein light chain family.

RefSeq proteins (1): NP_542408* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001372	Dynein_light_chain_typ-1/2	Family
IPR019763	Dynein_light_1/2_CS	Conserved_site
IPR037177	DLC_sf	Homologous_superfamily

Pfam: PF01221

UniProt features (9 total): strand 4, helix 2, chain 1, site 1, turn 1

Structure

Experimental structures (PDB)

4 structures.

PDB	Method	Resolution (Å)
2XQQ	X-RAY DIFFRACTION	1.31
4D07	X-RAY DIFFRACTION	1.85
7CNU	X-RAY DIFFRACTION	2
3P8M	X-RAY DIFFRACTION	2.9

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q96FJ2-F1	95.73	0.94

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Catalytic / active sites (1): 41 (interaction with myosin v motor complex)

Function

Pathways and Gene Ontology

Reactome pathways

15 pathways

ID	Pathway
R-HSA-139910	Activation of BMF and translocation to mitochondria
R-HSA-141444	Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-1632852	Macroautophagy
R-HSA-2132295	MHC class II antigen presentation
R-HSA-2467813	Separation of Sister Chromatids
R-HSA-2500257	Resolution of Sister Chromatid Cohesion
R-HSA-3371497	HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand
R-HSA-5620924	Intraflagellar transport
R-HSA-5663220	RHO GTPases Activate Formins
R-HSA-6807878	COPI-mediated anterograde transport
R-HSA-6811436	COPI-independent Golgi-to-ER retrograde traffic
R-HSA-68877	Mitotic Prometaphase
R-HSA-9609690	HCMV Early Events
R-HSA-9646399	Aggrephagy
R-HSA-9648025	EML4 and NUDC in mitotic spindle formation

MSigDB gene sets: 283 (showing top): GSE18804_BRAIN_VS_COLON_TUMORAL_MACROPHAGE_DN, GOBP_CHROMOSOME_ORGANIZATION, GOBP_REGULATION_OF_CELL_CYCLE_CHECKPOINT, REACTOME_ADAPTIVE_IMMUNE_SYSTEM, GOBP_REGULATION_OF_NUCLEAR_DIVISION, GOBP_CELL_CYCLE_PHASE_TRANSITION, ACEVEDO_NORMAL_TISSUE_ADJACENT_TO_LIVER_TUMOR_DN, GOBP_CHROMOSOME_SEPARATION, REACTOME_MEMBRANE_TRAFFICKING, GOCC_MICROTUBULE_ORGANIZING_CENTER, MONNIER_POSTRADIATION_TUMOR_ESCAPE_UP, GOBP_NEGATIVE_REGULATION_OF_CELLULAR_COMPONENT_ORGANIZATION, GOBP_POSITIVE_REGULATION_OF_INTRACELLULAR_TRANSPORT, SENESE_HDAC1_AND_HDAC2_TARGETS_DN, WEI_MYCN_TARGETS_WITH_E_BOX

GO Biological Process (1): microtubule-based process (GO:0007017)

GO Molecular Function (5): identical protein binding (GO:0042802), protein-containing complex binding (GO:0044877), dynein intermediate chain binding (GO:0045505), scaffold protein binding (GO:0097110), protein binding (GO:0005515)

GO Cellular Component (19): nucleus (GO:0005634), centrosome (GO:0005813), cytosol (GO:0005829), cytoskeleton (GO:0005856), cytoplasmic dynein complex (GO:0005868), microtubule (GO:0005874), plasma membrane (GO:0005886), cilium (GO:0005929), postsynaptic density (GO:0014069), membrane (GO:0016020), myosin V complex (GO:0031475), ciliary tip (GO:0097542), 9+0 non-motile cilium (GO:0097731), postsynapse (GO:0098794), glutamatergic synapse (GO:0098978), cytoplasm (GO:0005737), microtubule associated complex (GO:0005875), microtubule cytoskeleton (GO:0015630), dynein complex (GO:0030286)

Reactome top-level categories

Rollup of top-14 pathways:

Category	Pathways
Mitotic Prometaphase	2
Activation of BH3-only proteins	1
Amplification of signal from the kinetochores	1
Autophagy	1
Adaptive Immune System	1
Mitotic Anaphase	1
Cellular responses to stress	1
Assembly of the 9+0 primary cilium	1
RHO GTPase Effectors	1
ER to Golgi Anterograde Transport	1
Golgi-to-ER retrograde transport	1
M Phase	1
HCMV Infection	1
Selective autophagy	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	5
protein binding	3
binding	2
microtubule cytoskeleton	2
synapse	2
cellular process	1
intracellular membrane-bounded organelle	1
centriole	1
microtubule organizing center	1
cytoplasm	1
intracellular membraneless organelle	1
dynein complex	1
polymeric cytoskeletal fiber	1
membrane	1
cell periphery	1
intraciliary transport particle	1
membrane-bounded organelle	1
plasma membrane bounded cell projection	1
asymmetric synapse	1
postsynaptic specialization	1
unconventional myosin complex	1
cilium	1
non-motile cilium	1
intracellular anatomical structure	1
protein-containing complex	1
cytoskeleton	1
microtubule associated complex	1
catalytic complex	1

Protein interactions and networks

STRING

2518 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
DYNLL2	DYNLT3	P51808	825
DYNLL2	DYNLRB2	Q8TF09	794
DYNLL2	DYNLRB1	Q9NP97	785
DYNLL2	DYNC2I2	Q96EX3	784
DYNLL2	DYNLT1	P63172	769
DYNLL2	DYNC2LI1	Q8TCX1	751
DYNLL2	DYNC1H1	Q14204	695
DYNLL2	GPHN	Q9NQX3	686
DYNLL2	DYNLT2B	Q8WW35	642
DYNLL2	DYNC2I1	Q8WVS4	631
DYNLL2	DYNC1I1	O14576	628
DYNLL2	DYNC1I2	Q13409	628
DYNLL2	DYNC1LI2	O43237	627
DYNLL2	SYP	P08247	582
DYNLL2	DYNC1LI1	Q9Y6G9	571

IntAct

216 interactions, top by confidence:

A	B	Type	Score
CSNK1A1	FAM83G	psi-mi:“MI:0914”(association)	0.900
OFD1	DYNLL1	psi-mi:“MI:0914”(association)	0.890
FAM117B	DYNLL2	psi-mi:“MI:0915”(physical association)	0.880
DYNLL2	XIAP	psi-mi:“MI:0915”(physical association)	0.850
DYNLL2	TLK1	psi-mi:“MI:0915”(physical association)	0.840
DNAL4	DYNLL2	psi-mi:“MI:0915”(physical association)	0.800
DYNLL2	HMBOX1	psi-mi:“MI:0915”(physical association)	0.800
HMBOX1	DYNLL2	psi-mi:“MI:0915”(physical association)	0.800
DYNLL2	DNAL4	psi-mi:“MI:0915”(physical association)	0.800
DYNLL2	HOMER3	psi-mi:“MI:0915”(physical association)	0.800
KANK2	DYNLL2	psi-mi:“MI:0915”(physical association)	0.800
HOMER3	DYNLL2	psi-mi:“MI:0915”(physical association)	0.800
SECISBP2L	DYNLL2	psi-mi:“MI:0915”(physical association)	0.740
DYNLL2	SECISBP2L	psi-mi:“MI:0915”(physical association)	0.740
STRN4	STRN	psi-mi:“MI:0914”(association)	0.730
TLK1	DYNLL1	psi-mi:“MI:0914”(association)	0.730
DYNLL1	BLTP3B	psi-mi:“MI:0914”(association)	0.730
CFTR	ESYT2	psi-mi:“MI:2364”(proximity)	0.710
LCA5	SSNA1	psi-mi:“MI:0914”(association)	0.700
MORN3	DYNLL2	psi-mi:“MI:0915”(physical association)	0.670

BioGRID (436): DYNLL2 (Two-hybrid), DYNLL2 (Two-hybrid), DYNLL2 (Two-hybrid), DYNLL2 (Two-hybrid), DYNLL2 (Two-hybrid), DYNLL2 (Two-hybrid), DYNLL2 (Two-hybrid), MORN3 (Two-hybrid), C12orf40 (Two-hybrid), CCDC36 (Two-hybrid), DYNLL2 (Affinity Capture-MS), DYNLL2 (Affinity Capture-MS), DYNLL2 (Two-hybrid), DYNLL2 (Two-hybrid), DYNLL2 (Two-hybrid)

ESM2 similar proteins: A0A3Q7I7R4, A7YW45, O02414, O14744, O77210, O94111, O96860, P22953, P25840, P26639, P27322, P48612, P61273, P61285, P63167, P63168, P63169, P63170, Q02647, Q03389, Q16WA6, Q21557, Q22799, Q24117, Q39580, Q3MHR3, Q3ZBV8, Q4R5M3, Q58DV0, Q5R698, Q5RCE3, Q5U567, Q5XHY5, Q5XIP1, Q5ZK01, Q6BZF8, Q6CWX4, Q6FUJ0, Q6NUA1, Q759T0

Diamond homologs: A4F4L4, O02414, O94111, O96015, O96860, P61273, P61285, P63167, P63168, P63169, P63170, Q02647, Q22799, Q24117, Q32KN5, Q39580, Q3MHR3, Q6BZF8, Q6CWX4, Q6FUJ0, Q759T0, Q78P75, Q86A88, Q94758, Q96FJ2, Q9D0M5, Q9DCM4, Q9UR05, Q21557, Q39579, Q94748

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
DYNLL2	down-regulates	AMBRA1	binding

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 151 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Aggrephagy	7	18.9×	1e-05
Regulation of PLK1 Activity at G2/M Transition	12	16.6×	4e-09
AURKA Activation by TPX2	9	14.9×	2e-06
Loss of Nlp from mitotic centrosomes	8	13.8×	1e-05
Loss of proteins required for interphase microtubule organization from the centrosome	8	13.8×	1e-05
Recruitment of mitotic centrosome proteins and complexes	8	11.8×	3e-05
COPI-independent Golgi-to-ER retrograde traffic	5	11.3×	4e-03
Anchoring of the basal body to the plasma membrane	9	11.1×	1e-05

GO biological processes:

GO term	Partners	Fold	FDR
protein phosphorylation	10	5.3×	8e-03
cell division	12	4.3×	8e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

7 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	5
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

492 predictions. Top by Δscore:

Variant	Effect	Δscore
17:58084308:G:GT	donor_gain	1.0000
17:58087077:T:TA	acceptor_gain	1.0000
17:58087077:TGTA:T	acceptor_loss	1.0000
17:58087078:GTA:G	acceptor_loss	1.0000
17:58087079:TAG:T	acceptor_loss	1.0000
17:58087080:A:AG	acceptor_gain	1.0000
17:58087080:A:G	acceptor_loss	1.0000
17:58087080:AGT:A	acceptor_gain	1.0000
17:58087081:G:GA	acceptor_gain	1.0000
17:58087081:GT:G	acceptor_gain	1.0000
17:58087081:GTG:G	acceptor_gain	1.0000
17:58087081:GTGT:G	acceptor_gain	1.0000
17:58087081:GTGTC:G	acceptor_gain	1.0000
17:58087180:G:GT	donor_gain	1.0000
17:58087199:G:GT	donor_gain	1.0000
17:58087219:G:GT	donor_gain	1.0000
17:58087219:GAAG:G	donor_gain	1.0000
17:58087220:A:T	donor_gain	1.0000
17:58087222:GG:G	donor_loss	1.0000
17:58089368:G:GT	donor_gain	1.0000
17:58083595:G:GT	donor_gain	0.9900
17:58083683:GGT:G	donor_loss	0.9900
17:58083684:GTGAG:G	donor_loss	0.9900
17:58084317:GTGT:G	donor_gain	0.9900
17:58087183:G:GG	donor_gain	0.9900
17:58087195:G:GT	donor_gain	0.9900
17:58089136:TTTTA:T	acceptor_loss	0.9900
17:58089137:TTTAG:T	acceptor_loss	0.9900
17:58089138:TTA:T	acceptor_loss	0.9900
17:58089139:TAGGA:T	acceptor_loss	0.9900

AlphaMissense

601 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
17:58087206:C:A	A39D	1.000
17:58087219:G:C	K43N	1.000
17:58087219:G:T	K43N	1.000
17:58089169:T:A	W54R	1.000
17:58089169:T:C	W54R	1.000
17:58089175:T:C	C56R	1.000
17:58089176:G:A	C56Y	1.000
17:58089177:T:G	C56W	1.000
17:58089184:G:C	G59R	1.000
17:58089184:G:T	G59C	1.000
17:58089185:G:A	G59D	1.000
17:58089185:G:T	G59V	1.000
17:58089193:T:C	F62L	1.000
17:58089195:T:A	F62L	1.000
17:58089195:T:G	F62L	1.000
17:58089197:G:A	G63D	1.000
17:58089206:T:A	V66D	1.000
17:58089211:C:G	H68D	1.000
17:58089260:T:C	L84P	1.000
17:58089265:T:C	F86L	1.000
17:58089267:C:A	F86L	1.000
17:58089267:C:G	F86L	1.000
17:58087117:G:C	K9N	0.999
17:58087117:G:T	K9N	0.999
17:58087151:G:C	A21P	0.999
17:58087163:G:C	A25P	0.999
17:58087164:C:A	A25D	0.999
17:58087217:A:G	K43E	0.999
17:58087218:A:T	K43M	0.999
17:58089148:G:C	D47H	0.999

dbSNP variants (sampled 300 via entrez): RS1000743691 (17:58090587 A>G), RS1001340612 (17:58091970 C>T), RS1001392536 (17:58091569 G>T), RS1001570314 (17:58085826 G>A), RS1002012086 (17:58089493 AT>A,ATT), RS1002110391 (17:58084033 G>A), RS1002183521 (17:58095007 G>A), RS1002278200 (17:58095271 T>C), RS1002344366 (17:58090556 G>A), RS1002400133 (17:58090294 G>A), RS1002947483 (17:58082277 A>G), RS1003008967 (17:58093475 A>G), RS1003286236 (17:58086944 C>G,T), RS1003293342 (17:58092576 C>T), RS1003496690 (17:58083371 G>A,C,T)

Disease associations

OMIM: gene MIM:608942 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST006585_1709	Blood protein levels	3.000000e-10

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL6067226 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

4 potent at pChembl≥5 of 4 total, top 4 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
7.09	Kd	80.89	nM	CHEMBL3752910
6.83	ED50	148	nM	CHEMBL3752910
6.60	Kd	250.5	nM	CHEMBL5653589
6.34	ED50	458.5	nM	CHEMBL5653589

PubChem BioAssay actives

2 with measured affinity, of 4 total; 2 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
4-methyl-3-[(1-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2148275: Binding affinity to human DYNLL2 incubated for 45 mins by Kinobead based pull down assay	kd	0.0809	uM
4-methyl-3-[(2-methyl-6-pyridin-3-ylpyrazolo[3,4-d]pyrimidin-4-yl)amino]-N-[3-(trifluoromethyl)phenyl]benzamide	2148275: Binding affinity to human DYNLL2 incubated for 45 mins by Kinobead based pull down assay	kd	0.2505	uM

CTD chemical–gene interactions

37 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
sodium arsenite	increases reaction, increases expression, affects binding	3
bisphenol A	increases expression	2
Leflunomide	decreases expression	2
Valproic Acid	affects expression, increases expression	2
Aflatoxin B1	increases expression, decreases methylation	2
triphenyl phosphate	affects expression	1
lead acetate	increases expression	1
benzo(e)pyrene	decreases methylation	1
aflatoxin B2	increases methylation	1
cupric chloride	increases expression	1
resorcinol	decreases expression	1
beta-methylcholine	affects expression	1
di-n-butylphosphoric acid	affects expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, increases expression	1
nutlin 3	affects cotreatment, increases secretion	1
bisphenol S	increases expression	1
LDN 193189	affects cotreatment, increases expression	1
(+)-JQ1 compound	increases expression	1
Temozolomide	increases expression	1
Arsenic	affects expression	1
Benzo(a)pyrene	increases expression	1
Copper	affects binding, decreases expression	1
Dactinomycin	affects cotreatment, increases secretion	1
Disulfiram	affects binding, decreases expression	1
Colforsin	increases expression	1
Ivermectin	decreases expression	1
Methapyrilene	decreases methylation	1
Phenobarbital	affects expression	1
Phthalic Acids	increases methylation	1
Smoke	decreases expression	1

ChEMBL screening assays

1 unique, capped per target: 1 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5651317	Binding	Binding affinity to human DYNLL2 incubated for 45 mins by Kinobead based pull down assay	NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family. — ChemMedChem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.