EID2B

Summary

EID2B (EP300 interacting inhibitor of differentiation 2B, HGNC:26796) is a protein-coding gene on chromosome 19q13.2, encoding EP300-interacting inhibitor of differentiation 2B (Q96D98). Acts as a repressor of MYOD-dependent transcription, glucocorticoid receptor-dependent transcription, and muscle differentiation.

Enables identical protein binding activity. Involved in negative regulation of DNA-templated transcription and negative regulation of myoblast differentiation. Located in nucleus.

Source: NCBI Gene 126272 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 26 total
• MANE Select transcript: NM_152361

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:26796
Approved symbol	EID2B
Name	EP300 interacting inhibitor of differentiation 2B
Location	19q13.2
Locus type	gene with protein product
Status	Approved
Aliases	EID-3, FLJ38944
Ensembl gene	ENSG00000176401
Ensembl biotype	protein_coding
OMIM	617355
Entrez	126272

Gene structure

Transcript identifiers

Ensembl transcripts: 2 — 1 protein_coding, 1 protein_coding_CDS_not_defined

ENST00000326282, ENST00000601837

RefSeq mRNA: 1 — MANE Select: NM_152361 NM_152361

CCDS: CCDS12539

Canonical transcript exons

ENST00000326282 — 1 exons

Exon	Start	End
ENSE00001268682	39530987	39532852

Expression profiles

Bgee: expression breadth ubiquitous, 206 present calls, max score 84.68.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 4.5122 / max 54.2742, expressed in 1536 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
180892	3.6377	1455
180893	0.8745	443

Top tissues by expression

248 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	84.68	gold quality
cerebellar cortex	UBERON:0002129	82.55	gold quality
cerebellar hemisphere	UBERON:0002245	82.55	gold quality
Brodmann (1909) area 9	UBERON:0013540	82.54	gold quality
right hemisphere of cerebellum	UBERON:0014890	82.34	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	82.20	gold quality
cerebellum	UBERON:0002037	82.13	gold quality
hypothalamus	UBERON:0001898	81.93	gold quality
prefrontal cortex	UBERON:0000451	81.68	gold quality
cerebellar vermis	UBERON:0004720	81.24	gold quality
pons	UBERON:0000988	80.72	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	80.58	gold quality
anterior cingulate cortex	UBERON:0009835	80.39	gold quality
nucleus accumbens	UBERON:0001882	80.35	gold quality
right frontal lobe	UBERON:0002810	80.13	gold quality
pituitary gland	UBERON:0000007	80.01	gold quality
adenohypophysis	UBERON:0002196	79.98	gold quality
neocortex	UBERON:0001950	79.25	gold quality
frontal cortex	UBERON:0001870	79.16	gold quality
cortical plate	UBERON:0005343	78.56	gold quality
cerebral cortex	UBERON:0000956	77.96	gold quality
brain	UBERON:0000955	77.79	gold quality
amygdala	UBERON:0001876	77.73	gold quality
forebrain	UBERON:0001890	77.63	gold quality
caudate nucleus	UBERON:0001873	76.78	gold quality
putamen	UBERON:0001874	76.35	gold quality
calcaneal tendon	UBERON:0003701	76.18	gold quality
ganglionic eminence	UBERON:0004023	76.17	gold quality
primary visual cortex	UBERON:0002436	76.05	gold quality
ventricular zone	UBERON:0003053	75.32	gold quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-MTAB-6142	no	71.66
E-CURD-6	no	24.62
E-ANND-3	no	1.90

Regulation

Is transcription factor: yes

Downstream targets (CollecTRI)

1 targets.

Target	Regulation
MYOD1	Repression

miRNA regulators (miRDB)

34 targeting EID2B, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-5692A	100.00	74.40	6850
HSA-MIR-4682	100.00	68.89	1258
HSA-MIR-4715-3P	99.98	66.03	670
HSA-MIR-4482-3P	99.98	72.50	3147
HSA-MIR-25-3P	99.98	74.60	1817
HSA-MIR-32-5P	99.98	75.21	1964
HSA-MIR-363-3P	99.98	74.72	1821
HSA-MIR-367-3P	99.98	74.83	1819
HSA-MIR-92A-3P	99.98	75.21	1960
HSA-MIR-92B-3P	99.98	75.25	1955
HSA-MIR-570-3P	99.96	72.41	4910
HSA-MIR-302E	99.96	70.74	2669
HSA-MIR-141-3P	99.94	72.79	2421
HSA-MIR-200A-3P	99.94	72.68	2420
HSA-MIR-369-3P	99.85	70.52	2264
HSA-MIR-4639-5P	99.81	67.37	1028
HSA-MIR-4517	99.76	69.19	1867
HSA-MIR-4320	99.75	65.80	793
HSA-MIR-8084	99.73	69.57	1760
HSA-MIR-4719	99.73	72.10	3329
HSA-MIR-548AV-5P	99.60	70.84	2107
HSA-MIR-548K	99.60	70.84	2107
HSA-MIR-8054	99.48	70.81	2084
HSA-MIR-6853-3P	99.36	70.79	1558
HSA-MIR-138-2-3P	98.91	68.33	1643
HSA-MIR-520G-3P	98.91	67.38	1914
HSA-MIR-520H	98.91	67.38	1914
HSA-MIR-4709-5P	98.51	67.25	1335
HSA-MIR-5088-3P	98.29	66.63	1310
HSA-MIR-3614-3P	97.81	67.15	582

Literature-anchored findings (GeneRIF, showing 1)

• EID-3 inhibits MyoD- and GRalpha-dependent transcription and blocked muscle differentiation in cultured cells. It also associates with class I HDACs. (PMID:15970276)

Cross-species orthologs

1 orthologs

Organism	Symbol	Gene ID
mus_musculus	Eid2b	ENSMUSG00000070705

Paralogs (2): EID2 (ENSG00000176396), EID1 (ENSG00000255302)

Protein

Protein identifiers

EP300-interacting inhibitor of differentiation 2B — Q96D98 (reviewed: Q96D98)

Alternative names: EID-2-like inhibitor of differentiation 3

All UniProt accessions (1): Q96D98

UniProt curated annotations — full annotation on UniProt →

Function. Acts as a repressor of MYOD-dependent transcription, glucocorticoid receptor-dependent transcription, and muscle differentiation.

Subunit / interactions. Homodimer and heterodimer with EID2. Interacts with HDAC1 and HDAC2.

Subcellular location. Nucleus.

Domain organisation. The C-terminal portion of EID2B is required for nuclear localization.

RefSeq proteins (1): NP_689574* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR033258	EID	Family

UniProt features (3 total): region of interest 2, chain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q96D98-F1	70.26	0.28

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 63 (showing top): TGCGCANK_UNKNOWN, GOBP_REGULATION_OF_MYOBLAST_DIFFERENTIATION, GOBP_MUSCLE_STRUCTURE_DEVELOPMENT, GOBP_NEGATIVE_REGULATION_OF_MYOBLAST_DIFFERENTIATION, GOBP_MYOBLAST_DIFFERENTIATION, KUUSELO_PANCREATIC_CANCER_19Q13_AMPLIFICATION, GOBP_NEGATIVE_REGULATION_OF_DEVELOPMENTAL_PROCESS, NUYTTEN_EZH2_TARGETS_DN, GOMF_TRANSCRIPTION_COREPRESSOR_ACTIVITY, GARGALOVIC_RESPONSE_TO_OXIDIZED_PHOSPHOLIPIDS_TURQUOISE_DN, GOBP_NEGATIVE_REGULATION_OF_NUCLEOBASE_CONTAINING_COMPOUND_METABOLIC_PROCESS, GOMF_TRANSCRIPTION_COREGULATOR_ACTIVITY, ZWANG_TRANSIENTLY_UP_BY_2ND_EGF_PULSE_ONLY, NRF1_Q6, GOMF_TRANSCRIPTION_REGULATOR_ACTIVITY

GO Biological Process (4): muscle organ development (GO:0007517), cell differentiation (GO:0030154), negative regulation of myoblast differentiation (GO:0045662), negative regulation of DNA-templated transcription (GO:0045892)

GO Molecular Function (3): transcription corepressor activity (GO:0003714), identical protein binding (GO:0042802), protein binding (GO:0005515)

GO Cellular Component (2): nucleus (GO:0005634), nucleoplasm (GO:0005654)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
animal organ development	1
muscle structure development	1
cellular developmental process	1
myoblast differentiation	1
negative regulation of cell differentiation	1
regulation of myoblast differentiation	1
DNA-templated transcription	1
regulation of DNA-templated transcription	1
negative regulation of RNA biosynthetic process	1
transcription coregulator activity	1
negative regulation of DNA-templated transcription	1
protein binding	1
binding	1
intracellular membrane-bounded organelle	1
nuclear lumen	1
cellular anatomical structure	1

Protein interactions and networks

STRING

216 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
EID2B	NFE4	Q86UQ8	599
EID2B	NSMCE3	Q96MG7	554
EID2B	EID3	Q8N140	542
EID2B	LRIT1	Q9P2V4	471
EID2B	NSMCE4A	Q9NXX6	412
EID2B	C12orf43	Q96C57	400
EID2B	XTBD1	Q96HQ2	400
EID2B	MFSD11	O43934	391
EID2B	CLMB	Q96GX8	374
EID2B	EID1	Q9Y6B2	367
EID2B	SPINK7	P58062	327
EID2B	TMEM50B	P56557	326
EID2B	ADCK2	Q7Z695	325
EID2B	ZBTB5	O15062	324
EID2B	AK9	Q5TCS8	322

IntAct

30 interactions, top by confidence:

A	B	Type	Score
EID2B	MAGEE1	psi-mi:“MI:0915”(physical association)	0.770
EID2B	CTNNA3	psi-mi:“MI:0915”(physical association)	0.560
	EID2B	psi-mi:“MI:0915”(physical association)	0.560
POGZ	EID2B	psi-mi:“MI:0915”(physical association)	0.560
ARSA	EID2B	psi-mi:“MI:0915”(physical association)	0.560
EID2B	MAGEA1	psi-mi:“MI:0915”(physical association)	0.400
EID2B	MAGEF1	psi-mi:“MI:0915”(physical association)	0.400
EID2B	NDN	psi-mi:“MI:0915”(physical association)	0.400
EID2B	HDAC1	psi-mi:“MI:0915”(physical association)	0.400
EID2B	EID2B	psi-mi:“MI:0915”(physical association)	0.400
EID2B	EID2	psi-mi:“MI:0915”(physical association)	0.400
EID2B	EID3	psi-mi:“MI:0915”(physical association)	0.400
MAGEE1	FYN	psi-mi:“MI:0914”(association)	0.350
EID2B	MAGEE1	psi-mi:“MI:0915”(physical association)	0.000
CTNNA3	EID2B	psi-mi:“MI:0915”(physical association)	0.000
EID2B		psi-mi:“MI:0915”(physical association)	0.000
POGZ	EID2B	psi-mi:“MI:0915”(physical association)	0.000
EID2B	ARSA	psi-mi:“MI:0915”(physical association)	0.000
EID2B	EEF1G	psi-mi:“MI:0915”(physical association)	0.000

BioGRID (14): MAGEE1 (Affinity Capture-MS), SHPK (Affinity Capture-MS), MAGEE1 (Affinity Capture-MS), EID2B (Two-hybrid), EID2B (Two-hybrid), EID2B (Two-hybrid), CTNNA3 (Two-hybrid), MAGEE1 (Two-hybrid), MAGEE1 (Affinity Capture-MS), EID2B (Affinity Capture-MS), EID2B (Affinity Capture-RNA), EID2B (Affinity Capture-Western), EID2B (Affinity Capture-Western), EID2B (Affinity Capture-Western)

ESM2 similar proteins: A8E1G1, B8NI18, F5HEF2, F5HGN8, F5HIC6, O36368, O95424, P03256, P04486, P05729, P06492, P09047, P09301, P0C6J0, P0C6U7, P0C6X6, P0DJX0, P10580, P12418, P12535, P16726, P21698, P22656, P24414, P24635, P25073, P25214, P28938, P28991, P52511, P52512, P68335, P68336, P83978, P89430, Q00028, Q00106, Q00335, Q13323, Q2GF15

Diamond homologs: Q17QW4, Q5RDL6, Q6X7S9, Q8N6I1, Q96D98, Q9Y6B2

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

26 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	24
Likely benign	2
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

315 predictions. Top by Δscore:

Variant	Effect	Δscore
19:39532418:C:A	donor_gain	0.9800
19:39532285:T:TA	donor_gain	0.9700
19:39532290:G:A	donor_gain	0.9400
19:39532289:TGCC:T	donor_gain	0.9300
19:39532235:A:C	donor_gain	0.9100
19:39532417:T:TA	donor_gain	0.9100
19:39531510:TATT:T	acceptor_gain	0.8900
19:39532291:C:CT	donor_gain	0.8700
19:39531514:C:CC	acceptor_gain	0.8600
19:39532288:TTGC:T	donor_gain	0.8500
19:39532336:AGGG:A	donor_gain	0.8400
19:39532435:T:TA	donor_gain	0.8400
19:39531512:TT:T	acceptor_gain	0.8300
19:39531677:CA:C	donor_gain	0.8300
19:39531991:ACG:A	donor_gain	0.8200
19:39531992:CGC:C	donor_gain	0.8200
19:39532511:C:CT	acceptor_gain	0.8200
19:39532248:G:A	donor_gain	0.8000
19:39531866:T:TA	donor_gain	0.7900
19:39531948:TGGG:T	donor_gain	0.7900
19:39532292:C:CT	donor_gain	0.7700
19:39532166:T:TA	donor_gain	0.7600
19:39532244:G:GA	donor_gain	0.7600
19:39532283:ATT:A	donor_gain	0.7400
19:39532305:A:AC	donor_gain	0.7300
19:39532319:A:AC	donor_gain	0.7300
19:39532423:G:A	donor_gain	0.7300
19:39532510:TC:T	acceptor_gain	0.7300
19:39532320:G:C	donor_gain	0.7200
19:39531852:T:C	donor_gain	0.7100

AlphaMissense

1034 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
19:39532440:A:C	F121L	0.987
19:39532440:A:T	F121L	0.987
19:39532441:A:C	F121C	0.987
19:39532442:A:G	F121L	0.987
19:39532441:A:G	F121S	0.986
19:39532407:A:C	F132L	0.983
19:39532407:A:T	F132L	0.983
19:39532409:A:G	F132L	0.983
19:39532419:C:A	R128S	0.978
19:39532419:C:G	R128S	0.978
19:39532451:G:T	R118S	0.977
19:39532486:A:G	I106T	0.975
19:39532365:A:C	F146L	0.974
19:39532365:A:T	F146L	0.974
19:39532367:A:G	F146L	0.974
19:39532415:C:G	A130P	0.973
19:39532420:C:A	R128M	0.971
19:39532450:C:G	R118P	0.970
19:39532429:C:T	C125Y	0.969
19:39532408:A:G	F132S	0.963
19:39532430:A:G	C125R	0.963
19:39532366:A:G	F146S	0.961
19:39532424:C:G	A127P	0.959
19:39532428:G:C	C125W	0.956
19:39532420:C:G	R128T	0.954
19:39532483:G:A	P107L	0.954
19:39532408:A:C	F132C	0.953
19:39532416:T:A	E129D	0.952
19:39532416:T:G	E129D	0.952
19:39532412:C:G	A131P	0.950

dbSNP variants (sampled 300 via entrez): RS1000412867 (19:39532551 C>G,T), RS1001216997 (19:39531085 C>G,T), RS1002893859 (19:39532570 G>A), RS1003222618 (19:39534095 T>G), RS1003253775 (19:39533672 C>T), RS1003335391 (19:39533319 C>G,T), RS1005304076 (19:39531994 C>G,T), RS1005995042 (19:39531681 A>G), RS1006110912 (19:39532057 CG>C), RS1008667390 (19:39533675 G>C), RS1008969107 (19:39533310 C>G), RS1009022949 (19:39534164 G>A,T), RS1009039009 (19:39531524 A>G), RS1009070231 (19:39531186 T>C,G), RS1010166579 (19:39534273 C>T)

Disease associations

OMIM: gene MIM:617355 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

35 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Valproic Acid	affects cotreatment, decreases expression	5
Aflatoxin B1	increases methylation, affects expression, increases expression	4
trichostatin A	affects cotreatment, decreases expression	3
Air Pollutants	affects expression, increases abundance, decreases expression	2
Benzo(a)pyrene	increases expression, increases methylation	2
Formaldehyde	decreases expression, increases expression	2
Phenylmercuric Acetate	affects cotreatment, decreases expression	2
Tobacco Smoke Pollution	decreases expression, increases expression	2
Cyclosporine	decreases expression, increases expression	2
methylmercuric chloride	decreases expression	1
di-n-butylphosphoric acid	affects expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, decreases expression	1
erucylphospho-N,N,N-trimethylpropylammonium	increases expression	1
dorsomorphin	affects cotreatment, decreases expression	1
jinfukang	affects cotreatment, increases expression	1
4-(4-((5-(4,5-dimethyl-2-nitrophenyl)-2-furanyl)methylene)-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-1-yl)benzoic acid	increases expression	1
Temozolomide	decreases expression	1
Sunitinib	increases expression	1
Cisplatin	affects cotreatment, increases expression	1
Ethyl Methanesulfonate	increases expression	1
Lead	affects expression	1
Methyl Methanesulfonate	increases expression	1
Ozone	affects expression, increases abundance	1
Phthalic Acids	increases methylation	1
Quercetin	decreases expression	1
Smoke	decreases expression	1
Tetrachlorodibenzodioxin	affects expression	1
Urethane	decreases expression	1
Gold Compounds	decreases methylation	1
Asbestos, Crocidolite	decreases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.