EIF1B

Summary

EIF1B (eukaryotic translation initiation factor 1B, HGNC:30792) is a protein-coding gene on chromosome 3p22.1, encoding Eukaryotic translation initiation factor 1b (O60739). Probably involved in translation.

Enables RNA binding activity. Predicted to be involved in regulation of translational initiation. Predicted to be part of eukaryotic 43S preinitiation complex.

Source: NCBI Gene 10289 — RefSeq curated summary.

At a glance

• GWAS associations: 1
• Clinical variants (ClinVar): 11 total
• MANE Select transcript: NM_005875

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:30792
Approved symbol	EIF1B
Name	eukaryotic translation initiation factor 1B
Location	3p22.1
Locus type	gene with protein product
Status	Approved
Aliases	GC20
Ensembl gene	ENSG00000114784
Ensembl biotype	protein_coding
Entrez	10289

Gene structure

Transcript identifiers

Ensembl transcripts: 4 — 3 retained_intron, 1 protein_coding

ENST00000232905, ENST00000462088, ENST00000487151, ENST00000488260

RefSeq mRNA: 1 — MANE Select: NM_005875 NM_005875

CCDS: CCDS2690

Canonical transcript exons

ENST00000232905 — 4 exons

Exon	Start	End
ENSE00000825747	40311970	40312424
ENSE00001030073	40309707	40309972
ENSE00001167219	40311470	40311571
ENSE00003538070	40310893	40311056

Expression profiles

Bgee: expression breadth ubiquitous, 294 present calls, max score 99.31.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 174.0310 / max 3842.7949, expressed in 1827 samples.

FANTOM5 promoters (5 alternative TSS)

Promoter ID	TPM avg	Samples expressed
36173	108.2803	1821
36175	26.1850	1810
36176	22.2835	1814
36174	17.0001	1791
36177	0.2821	114

Top tissues by expression

295 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
heart right ventricle	UBERON:0002080	99.31	gold quality
adult organism	UBERON:0007023	99.15	gold quality
cardiac ventricle	UBERON:0002082	99.13	gold quality
heart left ventricle	UBERON:0002084	99.13	gold quality
pons	UBERON:0000988	99.11	gold quality
myocardium	UBERON:0002349	99.09	gold quality
left ventricle myocardium	UBERON:0006566	99.09	gold quality
cardiac atrium	UBERON:0002081	99.05	gold quality
right atrium auricular region	UBERON:0006631	99.05	gold quality
endothelial cell	CL:0000115	98.95	gold quality
substantia nigra pars compacta	UBERON:0001965	98.86	gold quality
heart	UBERON:0000948	98.81	gold quality
apex of heart	UBERON:0002098	98.81	gold quality
lateral nuclear group of thalamus	UBERON:0002736	98.81	gold quality
cardiac muscle of right atrium	UBERON:0003379	98.76	gold quality
vena cava	UBERON:0004087	98.75	gold quality
ganglionic eminence	UBERON:0004023	98.69	gold quality
superior vestibular nucleus	UBERON:0007227	98.69	gold quality
trabecular bone tissue	UBERON:0002483	98.68	gold quality
skeletal muscle tissue of biceps brachii	UBERON:0004502	98.64	gold quality
pigmented layer of retina	UBERON:0001782	98.62	gold quality
Brodmann (1909) area 23	UBERON:0013554	98.60	gold quality
dorsal root ganglion	UBERON:0000044	98.59	gold quality
substantia nigra pars reticulata	UBERON:0001966	98.52	gold quality
trigeminal ganglion	UBERON:0001675	98.45	gold quality
biceps brachii	UBERON:0001507	98.42	gold quality
cortical plate	UBERON:0005343	98.42	gold quality
cartilage tissue	UBERON:0002418	98.40	gold quality
postcentral gyrus	UBERON:0002581	98.37	gold quality
ventricular zone	UBERON:0003053	98.37	gold quality

Single-cell (SCXA)

Detected in 5 experiment(s), a significant marker in 4.

Experiment	Marker?	Max mean expression
E-MTAB-9221	yes	11.33
E-MTAB-10042	yes	5.99
E-MTAB-8410	yes	3.90
E-HCAD-5	no	7.70
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

49 targeting EIF1B, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-3613-3P	100.00	76.36	7965
HSA-MIR-3646	100.00	73.56	5283
HSA-MIR-196A-1-3P	99.99	72.15	2772
HSA-MIR-513B-5P	99.99	69.96	2150
HSA-MIR-511-3P	99.99	68.85	1467
HSA-MIR-4282	99.99	75.36	6408
HSA-MIR-3065-5P	99.97	71.56	3281
HSA-MIR-515-5P	99.92	69.82	2343
HSA-MIR-519E-5P	99.92	69.62	2358
HSA-MIR-3529-3P	99.90	73.55	3045
HSA-MIR-3686	99.90	70.53	2432
HSA-MIR-1323	99.83	69.89	2471
HSA-MIR-374C-5P	99.80	72.06	2910
HSA-MIR-655-3P	99.80	72.19	2909
HSA-MIR-548O-3P	99.74	69.30	2228
HSA-MIR-4719	99.73	72.10	3329
HSA-MIR-4802-3P	99.72	70.13	1273
HSA-MIR-494-3P	99.70	71.45	2795
HSA-MIR-7161-5P	99.68	68.92	1592
HSA-MIR-548U	99.65	67.78	1463
HSA-MIR-1303	99.65	69.77	1662
HSA-MIR-587	99.64	70.86	2611
HSA-MIR-4261	99.59	70.30	3415
HSA-MIR-6513-3P	99.59	69.77	1102
HSA-MIR-5571-5P	99.49	66.99	1764
HSA-MIR-147B-5P	99.45	70.62	2432
HSA-MIR-6507-3P	99.35	67.32	1059
HSA-MIR-580-5P	99.28	70.94	1776
HSA-MIR-3978	99.24	68.39	2201
HSA-MIR-223-5P	99.24	68.82	1206

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	eif1b	ENSDARG00000029445
mus_musculus	Eif1b	ENSMUSG00000006941
rattus_norvegicus	Eif1b	ENSRNOG00000018848
caenorhabditis_elegans		WBGENE00020868

Paralogs (1): EIF1 (ENSG00000173812)

Protein

Protein identifiers

Eukaryotic translation initiation factor 1b — O60739 (reviewed: O60739)

Alternative names: Protein translation factor SUI1 homolog GC20

All UniProt accessions (2): O60739, Q6FG85

UniProt curated annotations — full annotation on UniProt →

Function. Probably involved in translation.

Similarity. Belongs to the SUI1 family.

RefSeq proteins (1): NP_005866* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001950	SUI1	Domain
IPR005874	SUI1_euk	Family
IPR036877	SUI1_dom_sf	Homologous_superfamily

Pfam: PF01253

UniProt features (6 total): modified residue 2, sequence conflict 2, initiator methionine 1, chain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-O60739-F1	80.41	0.29

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (2): 2, 9

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 157 (showing top): YAO_TEMPORAL_RESPONSE_TO_PROGESTERONE_CLUSTER_10, ENK_UV_RESPONSE_KERATINOCYTE_UP, GOBP_TRANSLATIONAL_INITIATION, GOMF_TRANSLATION_INITIATION_FACTOR_ACTIVITY, GOBP_TRANSLATION, GOBP_POST_TRANSCRIPTIONAL_REGULATION_OF_GENE_EXPRESSION, GROSS_HYPOXIA_VIA_HIF1A_DN, chr3p22, LASTOWSKA_NEUROBLASTOMA_COPY_NUMBER_DN, HU_GENOTOXIC_DAMAGE_24HR, ACEVEDO_LIVER_CANCER_UP, LEE_CALORIE_RESTRICTION_MUSCLE_DN, ATGTTTC_MIR494, GOBP_REGULATION_OF_TRANSLATION, GOBP_REGULATION_OF_TRANSLATIONAL_INITIATION

GO Biological Process (5): regulation of translational initiation (GO:0006446), translation (GO:0006412), translational initiation (GO:0006413), regulation of gene expression (GO:0010468), regulation of primary metabolic process (GO:0080090)

GO Molecular Function (4): RNA binding (GO:0003723), translation initiation factor activity (GO:0003743), ribosomal small subunit binding (GO:0043024), protein binding (GO:0005515)

GO Cellular Component (1): eukaryotic 43S preinitiation complex (GO:0016282)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
translational initiation	3
regulation of translation	1
peptidyltransferase activity	1
translational elongation	1
translational termination	1
macromolecule biosynthetic process	1
protein metabolic process	1
protein biosynthetic process	1
formation of translation initiation ternary complex	1
translation	1
metabolic process	1
gene expression	1
regulation of macromolecule biosynthetic process	1
regulation of metabolic process	1
primary metabolic process	1
nucleic acid binding	1
translation factor activity	1
ribosome binding	1
binding	1
cytosolic small ribosomal subunit	1
cytosolic translation preinitiation complex	1

Protein interactions and networks

STRING

2073 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
EIF1B	ATP5IF1	Q9UII2	609
EIF1B	LMCD1	Q9NZU5	571
EIF1B	EIF3K	Q9UBQ5	484
EIF1B	EIF2S2	P20042	480
EIF1B	MRPS21	P82921	480
EIF1B	EIF5B	O60841	454
EIF1B	EIF5	P55010	453
EIF1B	LTA4H	P09960	453
EIF1B	ECM1	Q16610	453
EIF1B	RBM23	Q86U06	448
EIF1B	HTR2B	P41595	447
EIF1B	RAB31	Q13636	446
EIF1B	EIF2S1	P05198	440
EIF1B	ROBO1	Q9Y6N7	435
EIF1B	EIF3J	O75822	430

IntAct

193 interactions, top by confidence:

A	B	Type	Score
UCHL1	EIF1B	psi-mi:“MI:0915”(physical association)	0.680
EIF1B	FUS	psi-mi:“MI:0915”(physical association)	0.680
EIF1B	DVL3	psi-mi:“MI:0915”(physical association)	0.560
EIF1B	SPG21	psi-mi:“MI:0915”(physical association)	0.560
EIF1B		psi-mi:“MI:0915”(physical association)	0.370
EIF1B	iglC2	psi-mi:“MI:0915”(physical association)	0.370
RHOB	EIF1B	psi-mi:“MI:0915”(physical association)	0.370
PEX19	EIF1B	psi-mi:“MI:0915”(physical association)	0.370
ATXN1	EIF1B	psi-mi:“MI:0915”(physical association)	0.370
EIF1B	DVL2	psi-mi:“MI:0915”(physical association)	0.370
CD74		psi-mi:“MI:0914”(association)	0.350
DDX60	G6PD	psi-mi:“MI:0914”(association)	0.350
EIF3B	EIF3CL	psi-mi:“MI:0914”(association)	0.350
NPM1	RPS3A	psi-mi:“MI:0914”(association)	0.350
PSPC1	MCRIP1	psi-mi:“MI:0914”(association)	0.350
RPS16	MCRIP1	psi-mi:“MI:0914”(association)	0.350
EIF1B	POTEI	psi-mi:“MI:0914”(association)	0.350
NPPB	ACOT7	psi-mi:“MI:0914”(association)	0.350
EIF1B	EIF3E	psi-mi:“MI:0915”(physical association)	0.000
EIF1B	EIF3A	psi-mi:“MI:0915”(physical association)	0.000
EIF1B	EIF3K	psi-mi:“MI:0915”(physical association)	0.000
EIF1B	ACTR3	psi-mi:“MI:0915”(physical association)	0.000
EIF1B	VEPH1	psi-mi:“MI:0915”(physical association)	0.000
EIF1B	EIF3H	psi-mi:“MI:0915”(physical association)	0.000
EIF1B	EIF3G	psi-mi:“MI:0915”(physical association)	0.000

BioGRID (59): TRIM38 (Two-hybrid), EIF1B (Co-fractionation), EIF1B (Co-fractionation), EIF2D (Co-fractionation), EIF3G (Co-fractionation), TJP2 (Co-fractionation), DVL2 (Two-hybrid), EIF3A (Affinity Capture-MS), EIF3L (Affinity Capture-MS), EIF3M (Affinity Capture-MS), EIF3F (Affinity Capture-MS), EIF3D (Affinity Capture-MS), EIF3E (Affinity Capture-MS), ACTR3 (Affinity Capture-MS), RPS11 (Affinity Capture-MS)

ESM2 similar proteins: A0A324, A1XQR9, A4FUI2, A5JSS2, A6MZM2, G1SHQ2, O09167, O14602, O35900, O60739, P20280, P25800, P41567, P46778, P47813, P48024, P49666, P51971, P61220, P62303, P62304, P62305, P62308, P62309, Q09028, Q0D5W6, Q0P5B3, Q2KIA3, Q3B8H4, Q3ZBL0, Q4R4X9, Q503U0, Q5E938, Q5RA42, Q5RBW7, Q5RFF4, Q60872, Q60972, Q6GVM3, Q6QN05

Diamond homologs: A0B635, A0RWW9, A2BIX0, A2SQ91, A3CX23, A4FWB4, A4YIU6, A6VGZ2, A7I9K6, A8AC05, A9A389, A9A9Q7, B0R868, B6YSM1, B8GDE2, B9LR02, C5A278, O26118, O29348, O60739, P08245, P0A2G7, P0A2G8, P14021, P41567, P42678, P45116, P48024, P51971, P58193, P61220, P79060, Q12UF1, Q2FTC8, Q2NFW4, Q3IU96, Q46F27, Q4JAE7, Q4R4X9, Q55397

SIGNOR signaling

2 interactions.

A	Effect	B	Mechanism
EIF1B	“up-regulates activity”	“40S cytosolic small ribosomal subunit”	relocalization
EIF1B	“down-regulates activity”	“60S cytosolic large ribosomal subunit”

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 176 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Formation of the ternary complex, and subsequently, the 43S complex	25	36.4×	8e-32
Translation initiation complex formation	26	33.4×	7e-32
Ribosomal scanning and start codon recognition	26	33.4×	7e-32
Eukaryotic Translation Elongation	13	24.5×	1e-13
Formation of a pool of free 40S subunits	32	24.2×	5e-34
L13a-mediated translational silencing of Ceruloplasmin expression	35	23.9×	7e-37
GTP hydrolysis and joining of the 60S ribosomal subunit	35	23.7×	7e-37
Antigen processing: Ub, ATP-independent proteasomal degradation	6	23.1×	2e-06

GO biological processes:

GO term	Partners	Fold	FDR
formation of cytoplasmic translation initiation complex	12	77.9×	1e-19
translational initiation	17	35.2×	8e-20
cytoplasmic translation	21	22.5×	6e-20
regulation of translational initiation	5	13.5×	4e-03
translation	22	13.1×	4e-16
ribosomal small subunit biogenesis	8	10.5×	2e-04
positive regulation of translation	6	7.9×	8e-03
protein folding	9	5.4×	5e-03

Disease & clinical

Clinical variants and AI predictions

ClinVar

11 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	6
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

374 predictions. Top by Δscore:

Variant	Effect	Δscore
3:40310881:A:AG	acceptor_gain	1.0000
3:40310882:T:G	acceptor_gain	1.0000
3:40310885:A:AG	acceptor_gain	1.0000
3:40310886:T:G	acceptor_gain	1.0000
3:40310891:A:AG	acceptor_gain	1.0000
3:40310892:G:GG	acceptor_gain	1.0000
3:40311456:AT:A	acceptor_gain	1.0000
3:40311457:T:TA	acceptor_gain	1.0000
3:40311460:A:AG	acceptor_gain	1.0000
3:40311461:C:G	acceptor_gain	1.0000
3:40311464:TTCCA:T	acceptor_loss	1.0000
3:40311465:TCCAG:T	acceptor_loss	1.0000
3:40311466:CCAG:C	acceptor_loss	1.0000
3:40311467:CAGAA:C	acceptor_loss	1.0000
3:40311468:A:AG	acceptor_gain	1.0000
3:40311468:AGAAA:A	acceptor_loss	1.0000
3:40311469:G:GT	acceptor_gain	1.0000
3:40311469:GA:G	acceptor_gain	1.0000
3:40311469:GAA:G	acceptor_gain	1.0000
3:40311469:GAAA:G	acceptor_gain	1.0000
3:40311469:GAAAT:G	acceptor_gain	1.0000
3:40311568:GGAG:G	donor_gain	1.0000
3:40311569:GAG:G	donor_gain	1.0000
3:40311569:GAGG:G	donor_gain	1.0000
3:40311570:AGGT:A	donor_loss	1.0000
3:40311571:GGTG:G	donor_loss	1.0000
3:40311572:G:GG	donor_gain	1.0000
3:40311573:T:G	donor_loss	1.0000
3:40310885:ATTC:A	acceptor_gain	0.9900
3:40310888:C:A	acceptor_gain	0.9900

AlphaMissense

751 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
3:40310962:G:C	R34T	1.000
3:40310963:A:C	R34S	1.000
3:40310963:A:T	R34S	1.000
3:40310972:A:C	Q37H	1.000
3:40310972:A:T	Q37H	1.000
3:40310974:G:C	R38P	1.000
3:40310980:G:A	G40D	1.000
3:40310983:G:C	R41T	1.000
3:40310983:G:T	R41I	1.000
3:40310984:A:C	R41S	1.000
3:40310984:A:T	R41S	1.000
3:40310985:A:G	K42E	1.000
3:40310987:G:C	K42N	1.000
3:40310987:G:T	K42N	1.000
3:40310992:T:C	L44P	1.000
3:40311001:T:A	V47D	1.000
3:40311051:A:G	K64E	1.000
3:40311053:A:C	K64N	1.000
3:40311053:A:T	K64N	1.000
3:40311480:G:A	C69Y	1.000
3:40311481:T:G	C69W	1.000
3:40311485:G:C	G71R	1.000
3:40311486:G:A	G71D	1.000
3:40311528:T:C	L85P	1.000
3:40311533:G:A	G87S	1.000
3:40311533:G:C	G87R	1.000
3:40311533:G:T	G87C	1.000
3:40311534:G:A	G87D	1.000
3:40311534:G:T	G87V	1.000
3:40311536:G:C	D88H	1.000

dbSNP variants (sampled 300 via entrez): RS1001009956 (3:40308614 T>C), RS1001519270 (3:40312166 T>C), RS1001629319 (3:40311308 C>A,T), RS1002083067 (3:40311669 G>A,C,T), RS1002086741 (3:40310114 C>A,T), RS1003351369 (3:40310201 C>A,G,T), RS1003710292 (3:40309967 C>T), RS1004026271 (3:40307998 A>G), RS1004975924 (3:40309214 C>G), RS1004980940 (3:40311738 T>C), RS1005273038 (3:40311290 T>C), RS1005338817 (3:40308517 A>G), RS1005438045 (3:40309448 T>C), RS1005557999 (3:40308195 A>G), RS1006137873 (3:40311824 T>A)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

1 associations (top):

Study	Trait	p-value
GCST010002_421	Refractive error	3.000000e-27

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

38 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Air Pollutants	decreases expression, increases abundance, increases expression	2
Cadmium	decreases expression, increases abundance, increases expression	2
Tretinoin	increases expression	2
Valproic Acid	increases expression, decreases expression	2
Cadmium Chloride	increases abundance, increases expression, decreases expression	2
Particulate Matter	increases abundance, increases expression, decreases expression	2
triphenyl phosphate	affects expression	1
bisphenol A	affects expression	1
arsenite	affects binding, increases reaction	1
sodium arsenite	increases expression	1
perfluorooctane sulfonic acid	increases expression	1
CGP 52608	affects binding, increases reaction	1
chloropicrin	affects expression	1
erucylphospho-N,N,N-trimethylpropylammonium	increases expression	1
abrine	increases expression	1
(4-amino-1,4-dihydro-3-(2-pyridyl)-5-thioxo-1,2,4-triazole)copper(II)	increases expression	1
eprenetapopt	affects expression, affects reaction	1
Air Pollutants, Occupational	increases expression	1
Benzo(a)pyrene	increases methylation	1
Caffeine	affects phosphorylation	1
Cisplatin	increases expression	1
Dichlorodiphenyl Dichloroethylene	decreases expression	1
Doxorubicin	decreases expression	1
Estradiol	decreases expression	1
Nickel	increases expression	1
Phenobarbital	affects expression	1
Phthalic Acids	increases methylation	1
Rotenone	decreases expression	1
Selenium	decreases expression	1
Theophylline	decreases expression	1

Cellosaurus cell lines

2 cell lines: 2 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_SL60	HAP1 EIF1B (-) 1	Cancer cell line	Male
CVCL_SL61	HAP1 EIF1B (-) 2	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.