FAM174B

Summary

FAM174B (family with sequence similarity 174 member B, HGNC:34339) is a protein-coding gene on chromosome 15q26.1, encoding Membrane protein FAM174B (Q3ZCQ3). Essential for Golgi structural integrity.

Involved in Golgi organization. Located in Golgi apparatus and plasma membrane.

Source: NCBI Gene 400451 — RefSeq curated summary.

At a glance

• GWAS associations: 5
• Clinical variants (ClinVar): 26 total
• Cancer driver (intOGen): activating (oncogene-like) across 3 cancer types
• MANE Select transcript: NM_207446

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:34339
Approved symbol	FAM174B
Name	family with sequence similarity 174 member B
Location	15q26.1
Locus type	gene with protein product
Status	Approved
Aliases	LOC400451, MGC102891
Ensembl gene	ENSG00000185442
Ensembl biotype	protein_coding
Entrez	400451

Gene structure

Transcript identifiers

Ensembl transcripts: 13 — 9 protein_coding, 3 protein_coding_CDS_not_defined, 1 retained_intron

ENST00000327355, ENST00000553393, ENST00000554082, ENST00000555064, ENST00000555613, ENST00000555696, ENST00000555748, ENST00000555971, ENST00000556824, ENST00000557398, ENST00000557480, ENST00000557545, ENST00000896008

RefSeq mRNA: 1 — MANE Select: NM_207446 NM_207446

CCDS: CCDS45355

Canonical transcript exons

ENST00000327355 — 3 exons

Exon	Start	End
ENSE00001314585	92655316	92655775
ENSE00002532558	92617448	92619459
ENSE00003459935	92630214	92630345

Expression profiles

Bgee: expression breadth ubiquitous, 251 present calls, max score 95.81.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 10.7188 / max 191.1658, expressed in 1196 samples.

FANTOM5 promoters (4 alternative TSS)

Promoter ID	TPM avg	Samples expressed
151626	9.9471	1149
151627	0.2888	162
151640	0.2883	151
151625	0.1946	106

Top tissues by expression

280 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
body of pancreas	UBERON:0001150	95.81	gold quality
islet of Langerhans	UBERON:0000006	94.50	gold quality
pancreas	UBERON:0001264	94.26	gold quality
pancreatic ductal cell	CL:0002079	93.50	gold quality
parotid gland	UBERON:0001831	93.27	gold quality
pigmented layer of retina	UBERON:0001782	92.11	gold quality
right lobe of thyroid gland	UBERON:0001119	91.44	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	91.23	gold quality
left lobe of thyroid gland	UBERON:0001120	91.21	gold quality
thyroid gland	UBERON:0002046	90.94	gold quality
body of stomach	UBERON:0001161	89.51	gold quality
right lung	UBERON:0002167	89.47	gold quality
stomach	UBERON:0000945	88.30	gold quality
pituitary gland	UBERON:0000007	88.29	gold quality
adenohypophysis	UBERON:0002196	88.17	gold quality
saliva-secreting gland	UBERON:0001044	88.02	gold quality
prostate gland	UBERON:0002367	87.84	gold quality
upper lobe of left lung	UBERON:0008952	87.58	gold quality
secondary oocyte	CL:0000655	87.37	gold quality
middle temporal gyrus	UBERON:0002771	87.21	gold quality
minor salivary gland	UBERON:0001830	87.13	gold quality
endothelial cell	CL:0000115	87.09	silver quality
upper lobe of lung	UBERON:0008948	86.97	gold quality
calcaneal tendon	UBERON:0003701	86.78	gold quality
oocyte	CL:0000023	86.70	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	86.36	gold quality
palpebral conjunctiva	UBERON:0001812	86.34	gold quality
rectum	UBERON:0001052	86.25	gold quality
right uterine tube	UBERON:0001302	85.92	gold quality
right testis	UBERON:0004534	85.00	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 2.

Experiment	Marker?	Max mean expression
E-GEOD-83139	yes	3.86
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): AR

miRNA regulators (miRDB)

94 targeting FAM174B, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-8485	100.00	77.57	4731
HSA-MIR-518D-5P	100.00	67.51	979
HSA-MIR-518E-5P	100.00	67.66	954
HSA-MIR-518F-5P	100.00	67.51	979
HSA-MIR-519A-5P	100.00	67.66	954
HSA-MIR-519B-5P	100.00	67.66	954
HSA-MIR-519C-5P	100.00	67.66	954
HSA-MIR-520C-5P	100.00	67.51	979
HSA-MIR-522-5P	100.00	67.66	954
HSA-MIR-523-5P	100.00	67.66	954
HSA-MIR-526A-5P	100.00	67.51	979
HSA-MIR-3689D	100.00	66.14	1181
HSA-MIR-6851-5P	100.00	65.63	1294
HSA-MIR-3613-3P	100.00	76.36	7965
HSA-MIR-6870-5P	99.99	68.55	2115
HSA-MIR-4534	99.99	66.58	1907
HSA-MIR-4715-3P	99.98	66.03	670
HSA-MIR-4723-5P	99.97	68.70	2034
HSA-MIR-5698	99.97	68.49	2029
HSA-MIR-7111-5P	99.97	68.48	2062
HSA-MIR-3065-5P	99.97	71.56	3281
HSA-MIR-4267	99.96	66.53	2368
HSA-MIR-6825-5P	99.96	69.81	3431
HSA-MIR-8082	99.95	67.27	1170
HSA-MIR-6835-3P	99.93	70.49	2904
HSA-MIR-145-5P	99.92	71.13	1836
HSA-MIR-5195-3P	99.92	70.92	1877
HSA-MIR-497-5P	99.92	71.83	2674
HSA-MIR-15A-5P	99.90	72.80	2787
HSA-MIR-15B-5P	99.90	72.78	2798

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	fam174b	ENSDARG00000068709
mus_musculus	Fam174b	ENSMUSG00000078670
rattus_norvegicus	Fam174b	ENSRNOG00000012369

Protein

Protein identifiers

Membrane protein FAM174B — Q3ZCQ3 (reviewed: Q3ZCQ3)

All UniProt accessions (6): G3V5D1, H3BP98, H3BPY1, H3BR69, H3BTJ4, Q3ZCQ3

UniProt curated annotations — full annotation on UniProt →

Function. Essential for Golgi structural integrity.

Subcellular location. Cell membrane. Golgi apparatus.

Induction. Up-regulated by Golgi stress-inducing agent nigericin.

Similarity. Belongs to the FAM174 family.

RefSeq proteins (1): NP_997329* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR009565	FAM174-like	Family

Pfam: PF06679

UniProt features (11 total): sequence conflict 3, topological domain 2, signal peptide 1, chain 1, transmembrane region 1, region of interest 1, glycosylation site 1, sequence variant 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q3ZCQ3-F1	63.88	0.06

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Glycosylation sites (1): 53

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 132 (showing top): YANG_BREAST_CANCER_ESR1_BULK_UP, ZHAN_MULTIPLE_MYELOMA_MF_UP, LINDGREN_BLADDER_CANCER_CLUSTER_3_DN, GTGCCTT_MIR506, ONKEN_UVEAL_MELANOMA_UP, SENGUPTA_NASOPHARYNGEAL_CARCINOMA_DN, GOBP_ENDOMEMBRANE_SYSTEM_ORGANIZATION, WANG_TARGETS_OF_MLL_CBP_FUSION_UP, CUI_TCF21_TARGETS_2_DN, HAN_SATB1_TARGETS_DN, PARENT_MTOR_SIGNALING_UP, GOBP_GOLGI_ORGANIZATION, CAIRO_LIVER_DEVELOPMENT_UP, CHARAFE_BREAST_CANCER_LUMINAL_VS_MESENCHYMAL_UP, DOANE_BREAST_CANCER_CLASSES_UP

GO Biological Process (1): Golgi organization (GO:0007030)

GO Molecular Function (0):

GO Cellular Component (3): Golgi apparatus (GO:0005794), plasma membrane (GO:0005886), membrane (GO:0016020)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
organelle organization	1
endomembrane system organization	1
cytoplasm	1
endomembrane system	1
intracellular membrane-bounded organelle	1
membrane	1
cell periphery	1
cellular anatomical structure	1

Protein interactions and networks

STRING

534 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
FAM174B	LYSMD4	Q5XG99	574
FAM174B	ST8SIA2	Q92186	523
FAM174B	FAM124B	Q9H5Z6	518
FAM174B	LYSMD3	Q7Z3D4	496
FAM174B	SCAMP4	Q969E2	477
FAM174B	NUDT8	Q8WV74	467
FAM174B	GAK	O14976	454
FAM174B	DEPDC1	Q5TB30	453
FAM174B	XXYLT1	Q8NBI6	445
FAM174B	LCN10	Q6JVE6	439
FAM174B	CHD2	O14647	437
FAM174B	RAD9A	Q99638	429
FAM174B	RGMA	Q96B86	428
FAM174B	TBC1D16	Q8TBP0	425
FAM174B	TSPO2	Q5TGU0	421

IntAct

0 interactions, top by confidence:

BioGRID (1): FAM174B (Co-fractionation)

ESM2 similar proteins: A0A8I5KY20, A2A9T0, A2IDD5, B0BNK9, B8ZZ34, C9JI98, C9JLR9, F5GYI3, O18734, P0CG25, P84157, Q0IIA6, Q0PHV7, Q0X0E2, Q13387, Q1RMK9, Q2M3D2, Q2TAM9, Q3ZCQ3, Q4VA45, Q673H1, Q69YZ2, Q6NS60, Q6P6N5, Q6PJ61, Q7Z6J2, Q80ZJ8, Q810I0, Q86SX3, Q86UD0, Q86XT2, Q8BNN1, Q8IUW3, Q8N4Y2, Q8N6N2, Q8QZV0, Q8R4T5, Q8TF61, Q8VCR9, Q8WXF8

Diamond homologs: Q1LVN1, Q1RMK9, Q3ZCQ3, Q5FVQ7, Q8K064, Q8TBP5, Q9D3L0, Q640B5, A0JPB5

SIGNOR signaling

0 interactions.

Disease & clinical

Cancer significance

From intOGen — cancer-driver classification: activating (oncogene-like) across 3 cancer types — MBL, PAST, PRAD.

Clinical variants and AI predictions

ClinVar

26 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	17
Likely benign	2
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

966 predictions. Top by Δscore:

Variant	Effect	Δscore
15:92619456:TTAC:T	acceptor_gain	1.0000
15:92619457:TAC:T	acceptor_gain	1.0000
15:92619458:ACC:A	acceptor_loss	1.0000
15:92619460:CTA:C	acceptor_loss	1.0000
15:92619461:T:A	acceptor_loss	1.0000
15:92630208:CTGTA:C	donor_loss	1.0000
15:92630210:GTAC:G	donor_loss	1.0000
15:92630211:TAC:T	donor_loss	1.0000
15:92630212:A:AT	donor_loss	1.0000
15:92630341:CCGAC:C	acceptor_gain	1.0000
15:92630342:CGAC:C	acceptor_gain	1.0000
15:92630342:CGACC:C	acceptor_gain	1.0000
15:92630343:GAC:G	acceptor_gain	1.0000
15:92630344:AC:A	acceptor_gain	1.0000
15:92630345:CC:C	acceptor_gain	1.0000
15:92630345:CCT:C	acceptor_loss	1.0000
15:92630346:C:CC	acceptor_gain	1.0000
15:92630347:T:A	acceptor_loss	1.0000
15:92619455:TTTAC:T	acceptor_gain	0.9900
15:92619460:C:CC	acceptor_gain	0.9900
15:92655310:GCCCA:G	donor_loss	0.9900
15:92655311:CCCAC:C	donor_loss	0.9900
15:92655312:CCACC:C	donor_loss	0.9900
15:92655313:CA:C	donor_loss	0.9900
15:92655315:C:CT	donor_loss	0.9900
15:92619458:AC:A	acceptor_gain	0.9800
15:92619459:CC:C	acceptor_gain	0.9800
15:92621955:C:CC	acceptor_gain	0.9800
15:92630212:A:AC	donor_gain	0.9800
15:92630213:C:CC	donor_gain	0.9800

AlphaMissense

1004 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
15:92630228:G:C	F154L	0.999
15:92630228:G:T	F154L	0.999
15:92630230:A:G	F154L	0.999
15:92630229:A:C	F154C	0.998
15:92630277:A:G	M138T	0.998
15:92630276:C:A	M138I	0.997
15:92630276:C:G	M138I	0.997
15:92630276:C:T	M138I	0.997
15:92630313:T:C	Y126C	0.997
15:92630314:A:G	Y126H	0.997
15:92630277:A:C	M138R	0.996
15:92630229:A:G	F154S	0.995
15:92630304:A:T	I129N	0.995
15:92630304:A:G	I129T	0.994
15:92630230:A:C	F154V	0.993
15:92630230:A:T	F154I	0.993
15:92630304:A:C	I129S	0.993
15:92630277:A:T	M138K	0.992
15:92630307:A:C	I128S	0.991
15:92630313:T:G	Y126S	0.991
15:92630268:A:G	L141P	0.990
15:92630268:A:T	L141Q	0.990
15:92630307:A:T	I128N	0.990
15:92630315:C:A	K125N	0.990
15:92630315:C:G	K125N	0.990
15:92630320:G:T	R124S	0.989
15:92630314:A:C	Y126D	0.988
15:92630242:C:G	D150H	0.987
15:92630307:A:G	I128T	0.986
15:92630310:T:A	D127V	0.986

dbSNP variants (sampled 300 via entrez): RS1000013663 (15:92636366 T>G), RS1000088523 (15:92636081 C>T), RS1000090357 (15:92653836 C>A,T), RS1000162831 (15:92638530 A>C), RS1000242620 (15:92656878 C>T), RS1000251557 (15:92628089 C>T), RS1000308394 (15:92621985 C>A), RS1000337397 (15:92633018 G>A), RS1000432628 (15:92626380 T>A,G), RS1000453538 (15:92633394 G>A), RS1000519094 (15:92655242 G>A,C), RS1000592886 (15:92645743 G>A), RS1000619733 (15:92650901 C>T), RS1000682092 (15:92638738 C>T), RS1000692729 (15:92645336 G>A)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

5 associations (top):

Study	Trait	p-value
GCST000918_2	HIV-1 susceptibility	7.000000e-06
GCST001521_13	Subcutaneous adipose tissue	3.000000e-06
GCST003542_96	Night sleep phenotypes	4.000000e-06
GCST007267_121	Systolic blood pressure	2.000000e-10
GCST007269_152	Pulse pressure	9.000000e-10

EFO canonical traits (3, from GWAS)

EFO ID	Trait name
EFO:0000180	HIV-1 infection
EFO:0006335	systolic blood pressure
EFO:0005763	pulse pressure measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

30 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Valproic Acid	affects cotreatment, decreases expression, affects expression	4
sodium arsenite	increases expression	2
Tunicamycin	increases expression	2
methylmercuric chloride	decreases expression	1
triphenyl phosphate	affects expression	1
trichostatin A	decreases expression	1
beta-lapachone	decreases expression	1
ferrous chloride	decreases expression	1
2-palmitoylglycerol	increases expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, decreases expression	1
belinostat	decreases expression	1
ICG 001	increases expression	1
abrine	decreases expression	1
dorsomorphin	affects cotreatment, decreases expression	1
(+)-JQ1 compound	decreases expression	1
4-(4-((5-(4,5-dimethyl-2-nitrophenyl)-2-furanyl)methylene)-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-1-yl)benzoic acid	increases expression	1
Benzo(a)pyrene	affects methylation, decreases methylation, increases methylation	1
Cadmium	increases abundance, increases expression	1
Coumestrol	decreases expression	1
Doxorubicin	increases expression	1
Naled	affects expression	1
Plant Oils	decreases expression	1
Silicon Dioxide	decreases expression	1
Dihydrotestosterone	increases expression	1
Cyclosporine	increases expression	1
Aflatoxin B1	decreases expression	1
Cadmium Chloride	increases abundance, increases expression	1
Thapsigargin	increases expression	1
Okadaic Acid	decreases expression	1
Vitamin K 3	affects expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.