GAGE4

Summary

GAGE4 (G antigen 4, HGNC:4101) is a protein-coding gene on chromosome Xp11.4-p11.2 not on reference assembly, encoding G antigen 4 (P0DSO3). Antigen, recognized on melanoma by autologous cytolytic T-lymphocytes.

This gene belongs to a family of genes that are expressed in a variety of tumors but not in normal tissues, except for the testis. The sequences of the family members are highly related but differ by scattered nucleotide substitutions. The antigenic peptide YYWPRPRRY, which is also encoded by several other family members, is recognized by autologous cytolytic T lymphocytes.

Source: NCBI Gene 2576 — RefSeq curated summary.

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:4101
Approved symbol	GAGE4
Name	G antigen 4
Location	Xp11.4-p11.2 not on reference assembly
Locus type	gene with protein product
Status	Approved
Aliases	CT4.4
OMIM	300597
Entrez	2576

Gene structure

Transcript identifiers

Ensembl transcripts: 0

RefSeq mRNA: 0 — MANE Select: None

Canonical transcript exons

None — 0 exons

Expression profiles

Top tissues by expression

0 total, by Bgee expression score (0-100, higher = more expressed):

Regulation

Is transcription factor: no

Cross-species orthologs

0 orthologs

Protein

Protein identifiers

G antigen 4 — P0DSO3 (reviewed: P0DSO3)

Alternative names: Cancer/testis antigen 4.4

All UniProt accessions (0):

UniProt curated annotations — full annotation on UniProt →

Function. Antigen, recognized on melanoma by autologous cytolytic T-lymphocytes.

Tissue specificity. Expressed in a variety of tumor tissues but not in normal tissues, except testis.

Miscellaneous. This gene belongs to a family of genes organized in clustered repeats. They have a high degree of predicted sequence identity, but differ by scattered single nucleotide substitution. Their sequences contain either the antigenic peptide YYWPRPRRY or YRPRPRRY which is recognized by cytotoxic T-cells.

Similarity. Belongs to the GAGE family.

RefSeq proteins (0): (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR008625	GAGE_fam	Family
IPR031320	GAGE	Domain

Pfam: PF05831

UniProt features (5 total): compositionally biased region 3, chain 1, region of interest 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-P0DSO3-F1	62.66	0.00

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 0 (showing top):

GO Biological Process (0):

GO Molecular Function (0):

GO Cellular Component (0):

Protein interactions and networks

STRING

1417 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
GAGE4	MAGEA1	P43355	961
GAGE4	MAGEA3	P43357	944
GAGE4	SAGE1	Q9NXZ1	714
GAGE4	DDX43	Q9NXZ2	681
GAGE4	CTAG2	O75638	672
GAGE4	MAGED4B	Q96JG8	666
GAGE4	MAGEA2B	P43356	662
GAGE4	MAGEA4	P43358	608
GAGE4	CTAG1A	P78358	604
GAGE4	SPANXA1	Q9NS26	581
GAGE4	XAGE1B	Q9HD64	577
GAGE4	MAGEA10	P43363	574
GAGE4	SSX1	Q16384	573
GAGE4	MAGEB1	P43366	571
GAGE4	MAGEB2	O15479	571

IntAct

0 interactions, top by confidence:

ESM2 similar proteins: A0A0U1RQG5, A1L429, A6NDE8, A6NER3, A6NGK3, E1AZ71, O08664, O60829, O75459, O76087, P0C2W7, P0CL80, P0CL81, P0CL82, P0DSO3, P0DTW1, P52651, P62521, P86478, P86479, P86480, P86481, P86496, Q13066, Q13069, Q13070, Q17QW4, Q28181, Q2T9P9, Q32PA2, Q4V321, Q4V326, Q5JQC4, Q5U2Y8, Q62100, Q63803, Q64256, Q6NT46, Q6X7S9, Q7Z2X7

Diamond homologs: A1L429, A6NDE8, A6NER3, A6NGK3, O75459, O76087, P0CL80, P0CL81, P0CL82, P0DSO3, P0DTW1, Q13066, Q13069, Q13070, Q4V321, Q4V326, Q6NT46, Q8WTP9, Q96GT9, Q9UEU5, Q8WWM1, Q5JUK9, Q5JRK9, Q7Z2X7, Q9HD64

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

0 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	0
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

0 predictions. Top by Δscore:

AlphaMissense

0 scored. Top likely-pathogenic:

Disease associations

OMIM: gene MIM:300597 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

4 total (human), top 4 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
FR900359	decreases phosphorylation	1
Decitabine	increases expression	1
Zoledronic Acid	decreases expression	1
Mitoxantrone	affects response to substance	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.