GARRE1

Summary

GARRE1 (granule associated Rac and RHOG effector 1, HGNC:29016) is a protein-coding gene on chromosome 19q13.11, encoding Granule associated Rac and RHOG effector protein 1 (O15063). Acts as an effector of RAC1.

Enables CCR4-NOT complex binding activity and small GTPase binding activity. Involved in Rac protein signal transduction. Located in P-body.

Source: NCBI Gene 9710 — RefSeq curated summary.

At a glance

• GWAS associations: 4
• Clinical variants (ClinVar): 201 total
• MANE Select transcript: NM_014686

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:29016
Approved symbol	GARRE1
Name	granule associated Rac and RHOG effector 1
Location	19q13.11
Locus type	gene with protein product
Status	Approved
Aliases	MiniBAR
Ensembl gene	ENSG00000166398
Ensembl biotype	protein_coding
OMIM	619335
Entrez	9710

Gene structure

Transcript identifiers

Ensembl transcripts: 11 — 7 protein_coding, 3 protein_coding_CDS_not_defined, 1 retained_intron

ENST00000299505, ENST00000585833, ENST00000588338, ENST00000588470, ENST00000588974, ENST00000589583, ENST00000592124, ENST00000899720, ENST00000899721, ENST00000932925, ENST00000932926

RefSeq mRNA: 1 — MANE Select: NM_014686 NM_014686

CCDS: CCDS12436

Canonical transcript exons

ENST00000299505 — 14 exons

Exon	Start	End
ENSE00000401525	34347877	34348042
ENSE00000696163	34327990	34328151
ENSE00000696208	34330189	34330347
ENSE00000696256	34333704	34333801
ENSE00000696302	34339867	34339992
ENSE00001160214	34349016	34349153
ENSE00001160225	34341422	34342455
ENSE00001160305	34299679	34300968
ENSE00001184959	34352647	34355566
ENSE00002452690	34327421	34327561
ENSE00002498111	34319907	34320116
ENSE00002536110	34327771	34327866
ENSE00003587601	34351514	34351592
ENSE00003699210	34254554	34254614

Expression profiles

Bgee: expression breadth ubiquitous, 142 present calls, max score 90.80.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 13.3040 / max 162.8225, expressed in 1755 samples.

FANTOM5 promoters (2 alternative TSS)

Promoter ID	TPM avg	Samples expressed
175115	12.2452	1754
175117	1.0588	249

Top tissues by expression

142 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
placenta	UBERON:0001987	90.80	gold quality
calcaneal tendon	UBERON:0003701	90.80	gold quality
colonic epithelium	UBERON:0000397	89.46	gold quality
skeletal muscle tissue	UBERON:0001134	88.24	gold quality
omental fat pad	UBERON:0010414	87.55	gold quality
endometrium	UBERON:0001295	87.33	gold quality
adipose tissue	UBERON:0001013	87.05	gold quality
right lung	UBERON:0002167	86.81	gold quality
tonsil	UBERON:0002372	86.60	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	86.55	gold quality
subcutaneous adipose tissue	UBERON:0002190	86.55	gold quality
muscle tissue	UBERON:0002385	86.51	gold quality
thoracic mammary gland	UBERON:0005200	86.24	gold quality
muscle organ	UBERON:0001630	86.11	gold quality
skeletal muscle organ	UBERON:0014892	86.11	gold quality
muscle of leg	UBERON:0001383	86.03	gold quality
gall bladder	UBERON:0002110	85.90	gold quality
gastrocnemius	UBERON:0001388	85.83	gold quality
smooth muscle tissue	UBERON:0001135	85.42	gold quality
lung	UBERON:0002048	85.33	gold quality
uterus	UBERON:0000995	84.95	gold quality
hindlimb stylopod muscle	UBERON:0004252	84.62	gold quality
ascending aorta	UBERON:0001496	84.50	gold quality
adrenal tissue	UBERON:0018303	84.41	gold quality
thoracic aorta	UBERON:0001515	84.36	gold quality
lower esophagus mucosa	UBERON:0035834	84.35	gold quality
uterine cervix	UBERON:0000002	84.31	gold quality
left uterine tube	UBERON:0001303	84.15	gold quality
popliteal artery	UBERON:0002250	83.95	gold quality
tibial artery	UBERON:0007610	83.95	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	10.34

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

173 targeting GARRE1, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-6867-5P	100.00	82.21	3464
HSA-MIR-1252-5P	100.00	69.80	2774
HSA-MIR-3163	100.00	77.23	8605
HSA-MIR-4692	100.00	67.32	2066
HSA-MIR-5011-5P	100.00	83.46	5820
HSA-MIR-4795-3P	100.00	74.62	4024
HSA-MIR-30A-5P	100.00	76.31	3233
HSA-MIR-30B-5P	100.00	76.29	3248
HSA-MIR-30C-5P	100.00	76.29	3248
HSA-MIR-30D-5P	100.00	76.32	3233
HSA-MIR-30E-5P	100.00	76.32	3242
HSA-MIR-190A-3P	100.00	80.35	5520
HSA-MIR-200B-3P	100.00	73.31	2693
HSA-MIR-200C-3P	100.00	73.35	2685
HSA-MIR-429	100.00	73.44	2698
HSA-MIR-8485	100.00	77.57	4731
HSA-LET-7A-3P	100.00	74.03	3932
HSA-LET-7B-3P	100.00	74.08	3913
HSA-LET-7F-1-3P	100.00	74.02	3928
HSA-MIR-98-3P	100.00	74.08	3907
HSA-MIR-126-5P	100.00	72.71	3180
HSA-MIR-340-5P	100.00	72.50	4437
HSA-MIR-656-3P	100.00	72.15	2788
HSA-MIR-29A-3P	100.00	73.11	1835
HSA-MIR-29B-3P	100.00	73.18	1833
HSA-MIR-29C-3P	100.00	73.15	1833
HSA-MIR-4514	99.99	67.10	1870
HSA-MIR-4282	99.99	75.36	6408
HSA-MIR-186-5P	99.99	70.83	3707
HSA-MIR-223-3P	99.99	70.14	1140

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	garre1	ENSDARG00000102796
danio_rerio	GARRE1	ENSDARG00000105288
mus_musculus	Garre1	ENSMUSG00000066571
rattus_norvegicus	Garre1	ENSRNOG00000021129

Protein

Protein identifiers

Granule associated Rac and RHOG effector protein 1 — O15063 (reviewed: O15063)

All UniProt accessions (3): O15063, K7EPA0, U3KPV0

UniProt curated annotations — full annotation on UniProt →

Function. Acts as an effector of RAC1. Associates with CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. May also play a role in miRNA silencing machinery.

Subunit / interactions. Interacts with AGO2 and TNRC6A.

Subcellular location. Cytoplasm. P-body.

RefSeq proteins (1): NP_055501* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR031813	DUF4745	Domain
IPR043385	GARRE1	Family

Pfam: PF15923

UniProt features (46 total): helix 21, strand 8, compositionally biased region 6, turn 5, region of interest 3, chain 1, modified residue 1, sequence conflict 1

Structure

Experimental structures (PDB)

2 structures.

PDB	Method	Resolution (Å)
8BUY	X-RAY DIFFRACTION	1.6
8BUX	X-RAY DIFFRACTION	1.86

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-O15063-F1	54.87	0.17

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (1): 723

Function

Pathways and Gene Ontology

Reactome pathways

4 pathways

ID	Pathway
R-HSA-9013149	RAC1 GTPase cycle
R-HSA-9013404	RAC2 GTPase cycle
R-HSA-9013408	RHOG GTPase cycle
R-HSA-9013423	RAC3 GTPase cycle

MSigDB gene sets: 215 (showing top): TGGTGCT_MIR29A_MIR29B_MIR29C, MYOGENIN_Q6, GOMF_GTPASE_BINDING, ATGTTAA_MIR302C, PATIL_LIVER_CANCER, KOYAMA_SEMA3B_TARGETS_UP, BRN2_01, OCT1_06, ATTCTTT_MIR186, TGTTTAC_MIR30A5P_MIR30C_MIR30D_MIR30B_MIR30E5P, USF_02, CUI_TCF21_TARGETS_2_DN, DBP_Q6, FOXJ2_02, CART1_01

GO Biological Process (1): Rac protein signal transduction (GO:0016601)

GO Molecular Function (3): small GTPase binding (GO:0031267), CCR4-NOT complex binding (GO:1905762), protein binding (GO:0005515)

GO Cellular Component (3): P-body (GO:0000932), cytosol (GO:0005829), cytoplasm (GO:0005737)

Reactome top-level categories

Rollup of top-1 pathways:

Category	Pathways
RHO GTPase cycle	4

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	2
small GTPase-mediated signal transduction	1
GTPase binding	1
protein-containing complex binding	1
binding	1
cytoplasmic ribonucleoprotein granule	1
cytoplasm	1
intracellular anatomical structure	1

Protein interactions and networks

STRING

678 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
GARRE1	RESF1	Q9HCM1	543
GARRE1	CZIB	Q9NWV4	447
GARRE1	RSAD1	Q9HA92	433
GARRE1	LRATD1	Q96KN4	425
GARRE1	DOP1A	Q5JWR5	408
GARRE1	DOP1B	Q9Y3R5	402
GARRE1	DPM3	Q9P2X0	395
GARRE1	LRATD2	Q96KN1	391
GARRE1	DPM2	O94777	386
GARRE1	DPM1	O60762	375
GARRE1	FAM133B	Q5BKY9	358
GARRE1	P0DMU3	P0DMU3	353
GARRE1	PKNOX1	P55347	350
GARRE1	FAM135A	Q9P2D6	338
GARRE1	GPATCH8	Q9UKJ3	326

IntAct

43 interactions, top by confidence:

A	B	Type	Score
YWHAG	BLTP3B	psi-mi:“MI:0914”(association)	0.640
YWHAG	BLTP3B	psi-mi:“MI:2364”(proximity)	0.640
GARRE1	CAMKK1	psi-mi:“MI:0915”(physical association)	0.620
YWHAB	BLTP3B	psi-mi:“MI:2364”(proximity)	0.610
YWHAB	BLTP3B	psi-mi:“MI:0914”(association)	0.610
YWHAH	BLTP3B	psi-mi:“MI:0914”(association)	0.570
YWHAH	BLTP3B	psi-mi:“MI:2364”(proximity)	0.570
GARRE1		psi-mi:“MI:0915”(physical association)	0.550
GARRE1	APOD	psi-mi:“MI:0914”(association)	0.530
YWHAZ	BLTP3B	psi-mi:“MI:0914”(association)	0.530
KRBOX4	ASXL2	psi-mi:“MI:0914”(association)	0.350
Wdr5	MGA	psi-mi:“MI:0914”(association)	0.350
MYH13	C1orf226	psi-mi:“MI:0914”(association)	0.350
UNC93B1		psi-mi:“MI:0914”(association)	0.350
M		psi-mi:“MI:0914”(association)	0.350
	DENND11	psi-mi:“MI:0914”(association)	0.350
RYBP	PIPSL	psi-mi:“MI:0914”(association)	0.350
RYBP	FAM186A	psi-mi:“MI:0914”(association)	0.350
YWHAB	BRAF	psi-mi:“MI:0914”(association)	0.350
YWHAG	BRAF	psi-mi:“MI:0914”(association)	0.350
ATG16L1	ESYT2	psi-mi:“MI:0914”(association)	0.350
DUSP16	MEIOC	psi-mi:“MI:0914”(association)	0.350
SLC7A9	CDC7	psi-mi:“MI:0914”(association)	0.350
MKS1	GARRE1	psi-mi:“MI:2364”(proximity)	0.270
MKS1	AIP	psi-mi:“MI:2364”(proximity)	0.270
CPEB1	CNOT1	psi-mi:“MI:2364”(proximity)	0.270
FHIP1B	MED19	psi-mi:“MI:2364”(proximity)	0.270
YWHAB	E2F8	psi-mi:“MI:2364”(proximity)	0.270

BioGRID (213): KIAA0355 (Affinity Capture-MS), KIAA0355 (Proximity Label-MS), KIAA0355 (Affinity Capture-MS), KIAA0355 (Affinity Capture-MS), KIAA0355 (Affinity Capture-MS), CAMKK1 (Affinity Capture-MS), ABI2 (Affinity Capture-MS), CLU (Affinity Capture-MS), APOD (Affinity Capture-MS), NCKAP1 (Affinity Capture-MS), MUCL1 (Affinity Capture-MS), KIAA0355 (Affinity Capture-RNA), KIAA0355 (Proximity Label-MS), KIAA0355 (Affinity Capture-MS), KIAA0355 (Affinity Capture-MS)

ESM2 similar proteins: A0JMD2, A2AWL7, A2VCZ5, A6H5Y1, B8JKP6, D3ZMK9, F1MJR8, F1QB81, O14513, O15063, O60284, P08651, P15822, P31629, P35547, P59598, Q03172, Q08050, Q13029, Q15468, Q2YDH8, Q3UHF7, Q499E5, Q4V7H1, Q5REU9, Q5SPL2, Q5SW75, Q5SWW4, Q5T5Y3, Q5VWQ0, Q5W1J6, Q60988, Q63755, Q6JPI3, Q6NRK3, Q6NWJ0, Q71F56, Q76I76, Q76I79, Q80TY4

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 41 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Activation of BAD and translocation to mitochondria	7	190.3×	2e-13
Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex	7	167.9×	3e-13
SARS-CoV-1 targets host intracellular signalling and regulatory pathways	7	167.9×	3e-13
Activation of BH3-only proteins	7	124.1×	3e-12
RHO GTPases activate PKNs	7	79.3×	7e-11
Intrinsic Pathway for Apoptosis	7	73.2×	1e-10
FOXO-mediated transcription	5	60.0×	2e-07
SARS-CoV-1-host interactions	7	43.9×	3e-09

GO biological processes:

GO term	Partners	Fold	FDR
protein targeting	5	53.9×	5e-06
intracellular protein localization	7	21.6×	5e-06

Disease & clinical

Clinical variants and AI predictions

ClinVar

201 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	170
Likely benign	8
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

3362 predictions. Top by Δscore:

Variant	Effect	Δscore
19:34254612:CAGGT:C	donor_loss	1.0000
19:34254614:GG:G	donor_loss	1.0000
19:34254615:G:GA	donor_loss	1.0000
19:34320075:G:GT	donor_gain	1.0000
19:34320084:G:GT	donor_gain	1.0000
19:34327558:GCAA:G	donor_gain	1.0000
19:34327559:CAAG:C	donor_loss	1.0000
19:34327561:AGTA:A	donor_loss	1.0000
19:34327562:G:GG	donor_gain	1.0000
19:34327562:G:T	donor_loss	1.0000
19:34327563:TAA:T	donor_loss	1.0000
19:34327860:TTTGC:T	donor_gain	1.0000
19:34328144:T:G	donor_gain	1.0000
19:34328148:GACG:G	donor_gain	1.0000
19:34330187:A:AG	acceptor_gain	1.0000
19:34330188:G:GG	acceptor_gain	1.0000
19:34330188:GC:G	acceptor_gain	1.0000
19:34330188:GCAT:G	acceptor_gain	1.0000
19:34330188:GCATA:G	acceptor_gain	1.0000
19:34330348:G:GG	donor_gain	1.0000
19:34339861:GCCTA:G	acceptor_loss	1.0000
19:34339862:CCTAG:C	acceptor_loss	1.0000
19:34339863:CTA:C	acceptor_loss	1.0000
19:34339864:TAG:T	acceptor_loss	1.0000
19:34339865:A:AG	acceptor_gain	1.0000
19:34339865:A:C	acceptor_loss	1.0000
19:34339866:G:GC	acceptor_loss	1.0000
19:34339866:G:GG	acceptor_gain	1.0000
19:34339866:GGT:G	acceptor_gain	1.0000
19:34339989:GAAGG:G	donor_loss	1.0000

AlphaMissense

7020 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
19:34330294:T:A	W404R	1.000
19:34330294:T:C	W404R	1.000
19:34333783:T:A	V448D	1.000
19:34333800:T:A	W454R	1.000
19:34333800:T:C	W454R	1.000
19:34300724:T:C	L84P	0.999
19:34300733:T:C	L87P	0.999
19:34300753:A:C	S94R	0.999
19:34300755:T:A	S94R	0.999
19:34300755:T:G	S94R	0.999
19:34300763:T:C	L97P	0.999
19:34300823:T:C	L117P	0.999
19:34300844:T:A	V124D	0.999
19:34300865:T:C	L131P	0.999
19:34300877:T:A	I135K	0.999
19:34300901:T:C	L143P	0.999
19:34300910:T:C	L146P	0.999
19:34319919:T:C	C170R	0.999
19:34319921:T:G	C170W	0.999
19:34327828:T:C	F302L	0.999
19:34327829:T:C	F302S	0.999
19:34327830:C:A	F302L	0.999
19:34327830:C:G	F302L	0.999
19:34328148:G:C	K367N	0.999
19:34328148:G:T	K367N	0.999
19:34330205:T:C	L374P	0.999
19:34330282:T:C	C400R	0.999
19:34330308:C:G	C408W	0.999
19:34330311:G:C	K409N	0.999
19:34330311:G:T	K409N	0.999

dbSNP variants (sampled 300 via entrez): RS1000018510 (19:34268784 G>A,C), RS1000058391 (19:34353711 G>A), RS1000066670 (19:34311665 C>T), RS1000079598 (19:34298081 A>G), RS1000089909 (19:34269784 T>G), RS1000111999 (19:34311088 G>T), RS1000138045 (19:34304680 C>A,G), RS1000221531 (19:34316988 T>C), RS1000256209 (19:34275027 T>C), RS1000286084 (19:34347434 C>T), RS1000295430 (19:34315864 A>T), RS1000355245 (19:34275265 C>T), RS1000382649 (19:34298184 G>A), RS1000382696 (19:34344930 C>G), RS1000396955 (19:34323141 C>T)

Disease associations

OMIM: gene MIM:619335 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

4 associations (top):

Study	Trait	p-value
GCST000556_2	Functional MRI	7.000000e-06
GCST006979_679	Heel bone mineral density	2.000000e-12
GCST007269_174	Pulse pressure	2.000000e-08
GCST008554_9	Atorvastatin-induced myopathy	5.000000e-06

EFO canonical traits (3, from GWAS)

EFO ID	Trait name
EFO:0004550	amygdala reactivity measurement
EFO:0009270	heel bone mineral density
EFO:0005763	pulse pressure measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

24 total (human), top 24 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
GSK-J4	increases expression	1
bisphenol A	decreases methylation	1
trichostatin A	affects expression	1
methylparaben	increases expression	1
ICG 001	increases expression	1
(4-amino-1,4-dihydro-3-(2-pyridyl)-5-thioxo-1,2,4-triazole)copper(II)	decreases expression	1
4-(4-((5-(4,5-dimethyl-2-nitrophenyl)-2-furanyl)methylene)-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-1-yl)benzoic acid	increases expression	1
Temozolomide	increases expression	1
Leflunomide	increases expression	1
Acetaminophen	increases expression	1
Air Pollutants	increases abundance, decreases expression	1
Benzo(a)pyrene	affects methylation	1
Cadmium	decreases expression, increases abundance	1
Caffeine	decreases phosphorylation	1
Doxorubicin	decreases expression	1
Tobacco Smoke Pollution	increases expression	1
Tretinoin	decreases expression	1
Urethane	increases expression	1
Valproic Acid	affects expression	1
Cyclosporine	increases expression	1
Antirheumatic Agents	increases expression	1
Cadmium Chloride	decreases expression, increases abundance	1
Copper Sulfate	decreases expression	1
Particulate Matter	decreases expression, increases abundance	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.