GOLGA8N

Summary

GOLGA8N (golgin A8 family member N, HGNC:44405) is a protein-coding gene on chromosome 15q13.3, encoding Golgin subfamily A member 8N (F8WBI6). It is a selective cancer dependency (DepMap: 28.0% of cell lines).

Predicted to be involved in Golgi organization. Predicted to be located in Golgi apparatus. Predicted to be active in Golgi cis cisterna; Golgi cisterna membrane; and cis-Golgi network.

Source: NCBI Gene 643699 — RefSeq curated summary.

At a glance

• Cancer dependency (DepMap): dependent in 28.0% of screened cell lines
• MANE Select transcript: NM_001282494

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:44405
Approved symbol	GOLGA8N
Name	golgin A8 family member N
Location	15q13.3
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000232653
Ensembl biotype	protein_coding
Entrez	643699

Gene structure

Transcript identifiers

Ensembl transcripts: 8 — 4 retained_intron, 2 protein_coding, 2 protein_coding_CDS_not_defined

ENST00000448387, ENST00000453952, ENST00000567075, ENST00000569536, ENST00000569659, ENST00000605665, ENST00000611209, ENST00000695847

RefSeq mRNA: 1 — MANE Select: NM_001282494 NM_001282494

CCDS: CCDS61578

Canonical transcript exons

ENST00000448387 — 19 exons

Exon	Start	End
ENSE00001694830	32599723	32599810
ENSE00001704010	32596519	32596599
ENSE00001754275	32600187	32600443
ENSE00001805626	32603186	32603286
ENSE00002203239	32598068	32598177
ENSE00002211505	32602013	32602088
ENSE00002215765	32600699	32600767
ENSE00002239036	32597451	32597489
ENSE00002265241	32597888	32597972
ENSE00002267283	32593456	32593601
ENSE00002271021	32599227	32599313
ENSE00002298046	32599420	32599527
ENSE00002317425	32603562	32603717
ENSE00002318601	32603369	32603466
ENSE00002320186	32597752	32597799
ENSE00003515587	32602520	32602611
ENSE00003549973	32596278	32596337
ENSE00003577356	32603803	32607237
ENSE00003671596	32595268	32595387

Expression profiles

Bgee: expression breadth ubiquitous, 134 present calls, max score 96.63.

Top tissues by expression

134 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
sural nerve	UBERON:0015488	96.63	gold quality
right lobe of thyroid gland	UBERON:0001119	92.02	gold quality
thyroid gland	UBERON:0002046	90.82	gold quality
left lobe of thyroid gland	UBERON:0001120	90.74	gold quality
body of pancreas	UBERON:0001150	90.46	gold quality
left ovary	UBERON:0002119	87.30	gold quality
pituitary gland	UBERON:0000007	87.27	gold quality
mucosa of stomach	UBERON:0001199	87.18	gold quality
ovary	UBERON:0000992	86.52	gold quality
bone marrow	UBERON:0002371	86.06	gold quality
left testis	UBERON:0004533	86.03	gold quality
right ovary	UBERON:0002118	85.83	gold quality
adenohypophysis	UBERON:0002196	85.76	gold quality
granulocyte	CL:0000094	85.74	gold quality
pancreas	UBERON:0001264	85.47	gold quality
testis	UBERON:0000473	85.32	gold quality
descending thoracic aorta	UBERON:0002345	85.15	gold quality
right testis	UBERON:0004534	84.88	gold quality
right coronary artery	UBERON:0001625	84.64	gold quality
tibial artery	UBERON:0007610	84.46	gold quality
popliteal artery	UBERON:0002250	84.45	gold quality
bone marrow cell	CL:0002092	84.24	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	84.14	gold quality
endocervix	UBERON:0000458	84.03	gold quality
left uterine tube	UBERON:0001303	83.71	gold quality
thoracic aorta	UBERON:0001515	83.65	gold quality
ascending aorta	UBERON:0001496	83.64	gold quality
uterine cervix	UBERON:0000002	83.45	gold quality
endometrium	UBERON:0001295	83.12	gold quality
body of uterus	UBERON:0009853	83.01	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	1.72

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

112 targeting GOLGA8N, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-4262	100.00	73.26	3931
HSA-MIR-656-3P	100.00	72.15	2788
HSA-MIR-3646	100.00	73.56	5283
HSA-MIR-5692A	100.00	74.40	6850
HSA-MIR-3662	99.99	73.82	5684
HSA-MIR-4282	99.99	75.36	6408
HSA-MIR-181A-5P	99.99	72.96	2995
HSA-MIR-181B-5P	99.99	72.97	2996
HSA-MIR-181C-5P	99.99	72.95	2996
HSA-MIR-181D-5P	99.99	73.04	2997
HSA-MIR-6077	99.99	68.04	2299
HSA-MIR-4531	99.99	69.70	3181
HSA-MIR-548C-3P	99.99	74.01	7587
HSA-MIR-520D-5P	99.98	73.34	4883
HSA-MIR-524-5P	99.98	73.43	4882
HSA-MIR-4482-3P	99.98	72.50	3147
HSA-MIR-507	99.97	70.11	1915
HSA-MIR-607	99.97	73.62	5593
HSA-MIR-557	99.96	70.01	1640
HSA-MIR-3910	99.95	71.13	2227
HSA-MIR-144-3P	99.94	73.98	2698
HSA-MIR-3143	99.93	71.96	3104
HSA-MIR-4760-3P	99.93	70.50	2385
HSA-MIR-205-3P	99.92	69.92	3165
HSA-MIR-589-3P	99.91	69.62	2088
HSA-MIR-1305	99.91	71.43	3443
HSA-MIR-124-3P	99.89	73.74	3043
HSA-MIR-506-3P	99.89	73.55	3057
HSA-MIR-548AZ-5P	99.83	69.94	3230
HSA-MIR-548T-5P	99.83	69.91	3220

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 28.0% of screened cell lines.

Cross-species orthologs

5 orthologs

Organism	Symbol	Gene ID
danio_rerio	golga2	ENSDARG00000063197
mus_musculus	Golga2	ENSMUSG00000002546
rattus_norvegicus	Golga2l1	ENSRNOG00000050262
drosophila_melanogaster	GM130	FBGN0034697
caenorhabditis_elegans		WBGENE00018017

Paralogs (18): GOLGA6D (ENSG00000140478), GOLGA8F (ENSG00000153684), GOLGA6A (ENSG00000159289), GOLGA2 (ENSG00000167110), GOLGA6C (ENSG00000167195), GOLGA8A (ENSG00000175265), GOLGA8Q (ENSG00000178115), GOLGA8J (ENSG00000179938), GOLGA8G (ENSG00000183629), GOLGA8R (ENSG00000186399), GOLGA8M (ENSG00000188626), GOLGA8O (ENSG00000206127), GOLGA6B (ENSG00000215186), GOLGA8B (ENSG00000215252), GOLGA8K (ENSG00000249931), GOLGA8T (ENSG00000261247), GOLGA8S (ENSG00000261739), GOLGA8H (ENSG00000261794)

Protein

Protein identifiers

Golgin subfamily A member 8N — F8WBI6 (reviewed: F8WBI6)

All UniProt accessions (2): F8WBI6, H3BMP0

UniProt curated annotations — full annotation on UniProt →

Similarity. Belongs to the GOLGA8 family.

RefSeq proteins (1): NP_001269423* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR024858	GOLGA	Family
IPR043937	GOLGA_C	Conserved_site
IPR043976	GOLGA_cons_dom	Domain

Pfam: PF15070, PF19046

UniProt features (9 total): region of interest 4, coiled-coil region 2, compositionally biased region 2, chain 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-F8WBI6-F1	71.19	0.39

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 43 (showing top): chr15q13, GOBP_ENDOMEMBRANE_SYSTEM_ORGANIZATION, NAKAMURA_TUMOR_ZONE_PERIPHERAL_VS_CENTRAL_DN, BILBAN_B_CLL_LPL_DN, RODRIGUES_THYROID_CARCINOMA_POORLY_DIFFERENTIATED_DN, GOBP_GOLGI_ORGANIZATION, GOCC_GOLGI_STACK, GOCC_GOLGI_CISTERNA, GOCC_CIS_GOLGI_NETWORK, GOCC_GOLGI_CISTERNA_MEMBRANE, GOCC_ORGANELLE_SUBCOMPARTMENT, MULLIGHAN_MLL_SIGNATURE_1_UP, RODRIGUES_THYROID_CARCINOMA_ANAPLASTIC_DN, KRIGE_RESPONSE_TO_TOSEDOSTAT_6HR_DN, GOCC_GOLGI_CIS_CISTERNA

GO Biological Process (1): Golgi organization (GO:0007030)

GO Molecular Function (1): protein binding (GO:0005515)

GO Cellular Component (4): Golgi cis cisterna (GO:0000137), cis-Golgi network (GO:0005801), Golgi cisterna membrane (GO:0032580), Golgi apparatus (GO:0005794)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
Golgi cisterna	2
intracellular membrane-bounded organelle	2
organelle organization	1
endomembrane system organization	1
binding	1
Golgi apparatus	1
organelle membrane	1
cytoplasm	1
endomembrane system	1

Protein interactions and networks

STRING

366 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
GOLGA8N	MTMR10	Q9NXD2	544
GOLGA8N	GTF3C6	Q969F1	518
GOLGA8N	OTUD7A	Q8TE49	474
GOLGA8N	GTF3C5	Q9Y5Q8	447
GOLGA8N	CCDC144NL	Q6NUI1	436
GOLGA8N	TRMT5	Q32P41	406
GOLGA8N	GPHRA	B7ZAQ6	399
GOLGA8N	CCDC82	Q8N4S0	396
GOLGA8N	PCYT2	Q99447	370
GOLGA8N	TRMT6	Q9UJA5	369
GOLGA8N	TRPM1	Q7Z4N2	354
GOLGA8N	TMED2	Q15363	350
GOLGA8N	DHX38	Q92620	350
GOLGA8N	COMMD2	Q86X83	349
GOLGA8N	KLF13	Q9Y2Y9	348
GOLGA8N	RAPGEFL1	Q9UHV5	348

IntAct

0 interactions, top by confidence:

BioGRID (2): GOLGA8N (Affinity Capture-RNA), GOLGA8N (Negative Genetic)

ESM2 similar proteins: A2VD12, A6NCC3, A6NN73, A6QLY7, D6RF30, E1U8D0, F8WBI6, H3BPF8, H3BQL2, H3BSY2, H3BV12, I6L899, O75154, P0DX52, P0DX53, P10354, Q0D2H9, Q0KK56, Q2NL23, Q2TAC2, Q2YDF7, Q3MI48, Q3TVI8, Q3UHU5, Q3UPH7, Q5DU05, Q5JYT7, Q5R6R3, Q5SX79, Q640L3, Q6P2H3, Q6PHN1, Q7Z591, Q7Z6P3, Q80VW7, Q8BWG4, Q8CB62, Q8CB87, Q8IY92, Q8N137

Diamond homologs: A0A1B0GV03, A1IH00, A6NC78, A6NCC3, A6NDK9, A6NDN3, A6NEF3, A6NEM1, A6NI86, A6NN73, A7E2F4, A8MQT2, A8MZA4, F8WBI6, H0YKK7, H0YM25, H3BPF8, H3BSY2, H3BV12, I6L899, P0CG33, P0CJ92, P0DX00, P0DX01, P0DX02, P0DX52, P0DX53, Q08379, Q62839, Q8N7Z2, Q8N9W4, Q921M4, Q9NYA3, A6NMD2, D6RF30, H3BQL2, Q0D2H9, Q9HBQ8

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

0 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	0
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

2685 predictions. Top by Δscore:

Variant	Effect	Δscore
15:32595266:A:AG	acceptor_gain	1.0000
15:32595267:G:GG	acceptor_gain	1.0000
15:32595338:G:GT	donor_gain	1.0000
15:32595383:GGGAT:G	donor_gain	1.0000
15:32595384:GGAT:G	donor_gain	1.0000
15:32595384:GGATG:G	donor_gain	1.0000
15:32595385:G:T	donor_gain	1.0000
15:32595385:GAT:G	donor_gain	1.0000
15:32595385:GATG:G	donor_gain	1.0000
15:32595388:G:GG	donor_gain	1.0000
15:32596276:A:AG	acceptor_gain	1.0000
15:32596277:G:GG	acceptor_gain	1.0000
15:32596481:A:AG	acceptor_gain	1.0000
15:32596482:C:G	acceptor_gain	1.0000
15:32596486:T:A	acceptor_gain	1.0000
15:32596492:A:AG	acceptor_gain	1.0000
15:32596493:C:G	acceptor_gain	1.0000
15:32596505:T:TA	acceptor_gain	1.0000
15:32596508:A:AG	acceptor_gain	1.0000
15:32596508:AT:A	acceptor_gain	1.0000
15:32596509:T:G	acceptor_gain	1.0000
15:32596509:T:TA	acceptor_gain	1.0000
15:32596512:A:AG	acceptor_gain	1.0000
15:32596513:C:G	acceptor_gain	1.0000
15:32596517:A:AG	acceptor_gain	1.0000
15:32596517:A:T	acceptor_loss	1.0000
15:32596518:G:GA	acceptor_gain	1.0000
15:32596518:GA:G	acceptor_gain	1.0000
15:32596518:GAGC:G	acceptor_gain	1.0000
15:32596518:GAGCC:G	acceptor_gain	1.0000

AlphaMissense

4164 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
15:32600277:T:C	L322P	0.912
15:32600256:T:C	L315P	0.866
15:32600244:T:C	L311P	0.841
15:32599497:G:C	W252C	0.808
15:32599497:G:T	W252C	0.808
15:32603630:T:C	F546L	0.808
15:32603632:C:A	F546L	0.808
15:32603632:C:G	F546L	0.808
15:32603943:T:C	F622L	0.807
15:32603945:T:A	F622L	0.807
15:32603945:T:G	F622L	0.807
15:32596577:T:C	L96P	0.803
15:32599495:T:A	W252R	0.799
15:32599495:T:C	W252R	0.799
15:32593590:G:C	A13P	0.792
15:32595273:A:C	K18N	0.791
15:32595273:A:T	K18N	0.791
15:32599775:T:C	L280P	0.789
15:32593595:G:C	K14N	0.782
15:32593595:G:T	K14N	0.782
15:32603255:T:C	F480L	0.781
15:32603257:C:A	F480L	0.781
15:32603257:C:G	F480L	0.781
15:32596598:T:C	L103S	0.780
15:32597482:T:C	L114P	0.779
15:32597895:T:C	I135T	0.774
15:32597925:T:C	L145P	0.768
15:32595269:T:C	L17S	0.764
15:32599517:T:C	M259T	0.757
15:32600329:A:C	Q339H	0.748

dbSNP variants (sampled 300 via entrez): RS1001240309 (15:32606310 TA>T), RS1001678139 (15:32607172 T>C), RS1002304943 (15:32603992 C>G), RS1003676513 (15:32595420 G>T), RS1005047471 (15:32606846 C>G,T), RS1005307914 (15:32598060 C>T), RS1005381421 (15:32603055 C>G), RS1006516981 (15:32597420 C>A), RS1006999894 (15:32597970 A>T), RS1008261511 (15:32593484 G>C), RS1008607408 (15:32592421 G>A,C,T), RS1009609364 (15:32604702 G>A), RS1009640393 (15:32606150 T>G), RS1009943825 (15:32595780 A>AG), RS1009976484 (15:32597410 A>T)

Disease associations

OMIM: gene `` | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

11 total (human), top 11 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
GSK-J4	decreases expression	1
triphenyl phosphate	affects expression	1
bisphenol A	increases expression	1
di-n-butylphosphoric acid	affects expression	1
abrine	decreases expression	1
Silicon Dioxide	decreases expression	1
Smoke	decreases expression	1
Tobacco Smoke Pollution	decreases expression	1
Asbestos, Crocidolite	decreases expression	1
Okadaic Acid	decreases expression	1
Particulate Matter	increases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.