HAUS2

Summary

HAUS2 (HAUS augmin like complex subunit 2, HGNC:25530) is a protein-coding gene on chromosome 15q15.2, encoding HAUS augmin-like complex subunit 2 (Q9NVX0). Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. It is a selective cancer dependency (DepMap: 26.3% of cell lines).

The protein encoded by this gene is a subunit of the augmin complex. The augmin complex plays a role in microtubule attachment to the kinetochore and central spindle formation.

Source: NCBI Gene 55142 — RefSeq curated summary.

At a glance

• GWAS associations: 2
• Clinical variants (ClinVar): 45 total
• Cancer dependency (DepMap): dependent in 26.3% of screened cell lines
• MANE Select transcript: NM_018097

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:25530
Approved symbol	HAUS2
Name	HAUS augmin like complex subunit 2
Location	15q15.2
Locus type	gene with protein product
Status	Approved
Aliases	FLJ10460, HsT17025
Ensembl gene	ENSG00000137814
Ensembl biotype	protein_coding
OMIM	613429
Entrez	55142

Gene structure

Transcript identifiers

Ensembl transcripts: 15 — 10 protein_coding, 5 nonsense_mediated_decay

ENST00000260372, ENST00000391623, ENST00000562398, ENST00000563479, ENST00000564279, ENST00000567640, ENST00000568846, ENST00000568876, ENST00000570178, ENST00000607467, ENST00000935098, ENST00000935099, ENST00000935100, ENST00000935101, ENST00000970518

RefSeq mRNA: 6 — MANE Select: NM_018097 NM_001130447, NM_001323629, NM_001323630, NM_001323631, NM_001323632, NM_018097

CCDS: CCDS10090, CCDS45247, CCDS81867

Canonical transcript exons

ENST00000260372 — 6 exons

Exon	Start	End
ENSE00000884150	42558198	42558290
ENSE00001924665	42566607	42569994
ENSE00002616258	42548838	42548965
ENSE00003545064	42561270	42561402
ENSE00003620542	42563749	42563857
ENSE00003697532	42559339	42559408

Expression profiles

Bgee: expression breadth ubiquitous, 285 present calls, max score 99.74.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 26.7711 / max 379.9191, expressed in 1817 samples.

FANTOM5 promoters (3 alternative TSS)

Promoter ID	TPM avg	Samples expressed
146276	25.0331	1816
146275	0.8936	586
146274	0.8444	223

Top tissues by expression

288 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
renal medulla	UBERON:0000362	99.74	gold quality
cardia of stomach	UBERON:0001162	99.67	gold quality
pylorus	UBERON:0001166	99.61	gold quality
ventral tegmental area	UBERON:0002691	99.61	gold quality
nipple	UBERON:0002030	99.60	gold quality
trigeminal ganglion	UBERON:0001675	99.58	gold quality
sperm	CL:0000019	99.54	gold quality
inferior vagus X ganglion	UBERON:0005363	99.54	gold quality
superior surface of tongue	UBERON:0007371	99.53	gold quality
subthalamic nucleus	UBERON:0001906	99.52	gold quality
superior vestibular nucleus	UBERON:0007227	99.52	gold quality
dorsal root ganglion	UBERON:0000044	99.48	gold quality
lateral globus pallidus	UBERON:0002476	99.46	silver quality
pericardium	UBERON:0002407	99.45	gold quality
buccal mucosa cell	CL:0002336	99.43	gold quality
endothelial cell	CL:0000115	99.37	silver quality
substantia nigra pars reticulata	UBERON:0001966	99.37	silver quality
male germ cell	CL:0000015	99.34	gold quality
substantia nigra pars compacta	UBERON:0001965	99.34	gold quality
periodontal ligament	UBERON:0008266	99.33	silver quality
lateral nuclear group of thalamus	UBERON:0002736	99.29	gold quality
saphenous vein	UBERON:0007318	99.23	gold quality
pharyngeal mucosa	UBERON:0000355	99.22	gold quality
urethra	UBERON:0000057	99.18	gold quality
pons	UBERON:0000988	99.17	gold quality
dorsal plus ventral thalamus	UBERON:0001897	99.14	gold quality
blood vessel layer	UBERON:0004797	99.00	gold quality
penis	UBERON:0000989	98.99	gold quality
body of tongue	UBERON:0011876	98.97	gold quality
synovial joint	UBERON:0002217	98.95	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	4.18

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

105 targeting HAUS2, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-8485	100.00	77.57	4731
HSA-MIR-3646	100.00	73.56	5283
HSA-MIR-3163	100.00	77.23	8605
HSA-MIR-4425	100.00	67.59	1049
HSA-MIR-4789-3P	99.99	70.75	2484
HSA-MIR-3662	99.99	73.82	5684
HSA-MIR-548C-3P	99.99	74.01	7587
HSA-MIR-548P	99.98	72.25	3784
HSA-MIR-548N	99.98	71.94	4170
HSA-MIR-12136	99.98	72.81	5713
HSA-MIR-4645-5P	99.98	65.81	1284
HSA-MIR-4775	99.98	75.00	6394
HSA-MIR-6891-5P	99.98	66.53	1372
HSA-MIR-548AN	99.97	70.91	2817
HSA-MIR-6778-3P	99.96	67.29	2693
HSA-MIR-570-3P	99.96	72.41	4910
HSA-MIR-3910	99.95	71.13	2227
HSA-MIR-545-3P	99.95	70.74	2783
HSA-MIR-651-3P	99.94	73.48	5177
HSA-MIR-9983-3P	99.94	71.48	3631
HSA-MIR-539-5P	99.93	70.30	2855
HSA-MIR-10527-5P	99.91	72.28	3754
HSA-MIR-5680	99.91	69.83	3421
HSA-MIR-153-5P	99.89	73.86	6317
HSA-MIR-548E-5P	99.89	72.73	4486
HSA-MIR-6780A-5P	99.88	66.69	2776
HSA-MIR-30A-3P	99.87	69.74	2928
HSA-MIR-30D-3P	99.87	69.92	2917
HSA-MIR-30E-3P	99.87	69.68	2942
HSA-MIR-3941	99.86	70.54	2735

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 26.3% of screened cell lines.

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	haus2	ENSDARG00000036781
mus_musculus	Haus2	ENSMUSG00000027285
rattus_norvegicus	Haus2	ENSRNOG00000048933
rattus_norvegicus		ENSRNOG00000076589

Protein

Protein identifiers

HAUS augmin-like complex subunit 2 — Q9NVX0 (reviewed: Q9NVX0)

Alternative names: Centrosomal protein of 27 kDa

All UniProt accessions (8): Q9NVX0, H3BP16, H3BP24, H3BQU2, H3BR32, H3BTG9, J3QT48, U3KQI5

UniProt curated annotations — full annotation on UniProt →

Function. Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.

Subunit / interactions. Component of the HAUS augmin-like complex. The complex interacts with the gamma-tubulin ring complex and this interaction is required for spindle assembly. Interacts with EML3 (phosphorylated at ‘Thr-881’).

Subcellular location. Cytoplasm. Cytoskeleton. Microtubule organizing center. Centrosome. Spindle.

Similarity. Belongs to the HAUS2 family.

Isoforms (3)

UniProt ID	Names	Canonical?
Q9NVX0-1	1	yes
Q9NVX0-2	2
Q9NVX0-3	3

RefSeq proteins (6): NP_001123919, NP_001310558, NP_001310559, NP_001310560, NP_001310561, NP_060567* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR026242	HAUS2_metazoa	Family
IPR028346	HAUS2	Family

Pfam: PF15003

UniProt features (5 total): splice variant 2, chain 1, coiled-coil region 1, sequence variant 1

Structure

Experimental structures (PDB)

1 structures.

PDB	Method	Resolution (Å)
7SQK	ELECTRON MICROSCOPY	8

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9NVX0-F1	80.95	0.44

Function

Pathways and Gene Ontology

Reactome pathways

7 pathways

ID	Pathway
R-HSA-2565942	Regulation of PLK1 Activity at G2/M Transition
R-HSA-380259	Loss of Nlp from mitotic centrosomes
R-HSA-380270	Recruitment of mitotic centrosome proteins and complexes
R-HSA-380284	Loss of proteins required for interphase microtubule organization from the centrosome
R-HSA-380320	Recruitment of NuMA to mitotic centrosomes
R-HSA-5620912	Anchoring of the basal body to the plasma membrane
R-HSA-8854518	AURKA Activation by TPX2

MSigDB gene sets: 182 (showing top): GOBP_REGULATION_OF_PROTEIN_POLYMERIZATION, GOBP_REGULATION_OF_MICROTUBULE_BASED_PROCESS, SHEPARD_CRASH_AND_BURN_MUTANT_UP, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_UP, GOBP_MICROTUBULE_NUCLEATION, GOBP_REGULATION_OF_CELLULAR_COMPONENT_BIOGENESIS, GOCC_MICROTUBULE_ORGANIZING_CENTER, WEI_MYCN_TARGETS_WITH_E_BOX, CADWELL_ATG16L1_TARGETS_DN, GOBP_REGULATION_OF_MICROTUBULE_POLYMERIZATION, GOCC_CENTROSOME, TCF11_01, COATES_MACROPHAGE_M1_VS_M2_UP, GOBP_REGULATION_OF_PROTEIN_CONTAINING_COMPLEX_ASSEMBLY, GOBP_ORGANELLE_ASSEMBLY

GO Biological Process (6): microtubule nucleation (GO:0007020), centrosome cycle (GO:0007098), regulation of microtubule nucleation (GO:0010968), spindle assembly (GO:0051225), cell division (GO:0051301), microtubule organizing center organization (GO:0031023)

GO Molecular Function (1): protein binding (GO:0005515)

GO Cellular Component (9): centrosome (GO:0005813), cytosol (GO:0005829), HAUS complex (GO:0070652), mitotic spindle microtubule (GO:1990498), cytoplasm (GO:0005737), spindle (GO:0005819), cytoskeleton (GO:0005856), microtubule (GO:0005874), spindle microtubule (GO:0005876)

Reactome top-level categories

Rollup of top-5 pathways:

Category	Pathways
G2/M Transition	2
Centrosome maturation	2
Loss of proteins required for interphase microtubule organization from the centrosome	1
Mitotic Prometaphase	1
Assembly of the 9+0 primary cilium	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
microtubule cytoskeleton organization	2
cellular anatomical structure	2
microtubule cytoskeleton	2
intracellular membraneless organelle	2
microtubule polymerization	1
cell cycle process	1
microtubule organizing center organization	1
microtubule nucleation	1
regulation of microtubule polymerization	1
spindle organization	1
chromosome segregation	1
membraneless organelle assembly	1
cellular process	1
microtubule-based process	1
cellular component organization	1
binding	1
centriole	1
microtubule organizing center	1
cytoplasm	1
microtubule associated complex	1
spindle microtubule	1
mitotic spindle	1
intracellular anatomical structure	1
polymeric cytoskeletal fiber	1
spindle	1
microtubule	1

Protein interactions and networks

STRING

902 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
HAUS2	HAUS6	Q7Z4H7	877
HAUS2	HAUS7	Q99871	848
HAUS2	HAUS1	Q96CS2	793
HAUS2	HAUS4	Q9H6D7	689
HAUS2	HAUS5	O94927	685
HAUS2	HAUS3	Q68CZ6	682
HAUS2	HAUS8	Q9BT25	678
HAUS2	NUMA1	Q14980	670
HAUS2	TUBG1	P23258	649
HAUS2	CEP290	O15078	646
HAUS2	PCNT	O95613	492
HAUS2	UBE2V2	Q15819	472
HAUS2	D6RC18	D6RC18	431
HAUS2	MRPS14	O60783	408
HAUS2	ISY1	Q9ULR0	398

IntAct

64 interactions, top by confidence:

A	B	Type	Score
MED4	MED19	psi-mi:“MI:2364”(proximity)	0.900
HAUS6	HAUS2	psi-mi:“MI:0915”(physical association)	0.880
HAUS2	HAUS6	psi-mi:“MI:0915”(physical association)	0.880
HAUS1	HAUS6	psi-mi:“MI:0915”(physical association)	0.810
HAUS1	HAUS5	psi-mi:“MI:0915”(physical association)	0.780
HAUS2	HAUS5	psi-mi:“MI:0914”(association)	0.730
HAUS6	HAUS5	psi-mi:“MI:0914”(association)	0.710
HAUS4	HAUS5	psi-mi:“MI:0914”(association)	0.640
ARFIP1	ARL1	psi-mi:“MI:0914”(association)	0.640
Haus4	HAUS5	psi-mi:“MI:0914”(association)	0.560
XRCC4	HAUS2	psi-mi:“MI:0915”(physical association)	0.560
Haus4	HAUS5	psi-mi:“MI:0915”(physical association)	0.560
SMC1A	PDS5B	psi-mi:“MI:0914”(association)	0.530
SYCE3	RER1	psi-mi:“MI:0914”(association)	0.530
KIAA0753	OFD1	psi-mi:“MI:2364”(proximity)	0.480
Haus6	HAUS5	psi-mi:“MI:0915”(physical association)	0.400
Haus1	GNAT3	psi-mi:“MI:0915”(physical association)	0.400
MAPK6	HAUS2	psi-mi:“MI:0915”(physical association)	0.370
Haus1	LTF	psi-mi:“MI:0914”(association)	0.350
HAUS2	SCAMP3	psi-mi:“MI:0914”(association)	0.350
Xpo1	IFT56	psi-mi:“MI:0914”(association)	0.350
HTT		psi-mi:“MI:0914”(association)	0.350
HAUS7	GOLIM4	psi-mi:“MI:0914”(association)	0.350
SYCE3	TRIM24	psi-mi:“MI:0914”(association)	0.350

BioGRID (174): HAUS2 (Affinity Capture-RNA), HAUS2 (Affinity Capture-MS), HAUS2 (Proximity Label-MS), HAUS2 (Proximity Label-MS), HAUS2 (Proximity Label-MS), HAUS2 (Proximity Label-MS), HAUS2 (Proximity Label-MS), HAUS2 (Proximity Label-MS), HAUS2 (Proximity Label-MS), HAUS2 (Proximity Label-MS), HAUS2 (Proximity Label-MS), HAUS2 (Proximity Label-MS), HAUS2 (Proximity Label-MS), HAUS2 (Proximity Label-MS), HAUS2 (Proximity Label-MS)

ESM2 similar proteins: A0A1B0GTZ2, A3RM20, A4UHQ4, A6H7E2, A6NGH7, A9JSR5, B0BK70, O55527, O74982, P04861, P04862, P06747, P0C137, P0C139, P0C142, P14253, P14254, P33493, P35940, P40167, P69479, P69480, P69738, Q0GBX8, Q13352, Q14BK3, Q2T9U9, Q2YDE5, Q32L17, Q3UYG1, Q4KLZ4, Q4VKV6, Q5I0J4, Q5RE16, Q66HB6, Q6AXY9, Q810N5, Q8IR45, Q8IYM0, Q8NCU1

Diamond homologs: Q5RE16, Q9CQS9, Q9NVX0

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
HAUS2	“form complex”	“HAUS complex”	binding

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 63 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Loss of Nlp from mitotic centrosomes	10	38.7×	4e-12
Loss of proteins required for interphase microtubule organization from the centrosome	10	38.7×	4e-12
AURKA Activation by TPX2	10	37.1×	5e-12
Recruitment of mitotic centrosome proteins and complexes	10	33.2×	1e-11
Anchoring of the basal body to the plasma membrane	12	33.1×	2e-13
Regulation of PLK1 Activity at G2/M Transition	10	30.9×	2e-11
Recruitment of NuMA to mitotic centrosomes	10	28.4×	4e-11

GO biological processes:

GO term	Partners	Fold	FDR
centrosome cycle	7	41.4×	5e-08
spindle assembly	5	38.9×	1e-05
cilium assembly	8	10.3×	7e-05
cell division	11	8.9×	4e-06

Disease & clinical

Clinical variants and AI predictions

ClinVar

45 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	32
Likely benign	1
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

555 predictions. Top by Δscore:

Variant	Effect	Δscore
15:42548962:TCAGG:T	donor_loss	1.0000
15:42548967:T:G	donor_loss	1.0000
15:42561267:T:G	acceptor_gain	1.0000
15:42561268:A:AG	acceptor_gain	1.0000
15:42561269:G:GA	acceptor_gain	1.0000
15:42561371:G:GT	donor_gain	1.0000
15:42561372:A:T	donor_gain	1.0000
15:42563743:TTTCA:T	acceptor_loss	1.0000
15:42563744:TTCA:T	acceptor_loss	1.0000
15:42563745:TCA:T	acceptor_loss	1.0000
15:42563746:CAGAT:C	acceptor_loss	1.0000
15:42563747:A:AC	acceptor_loss	1.0000
15:42563747:A:AG	acceptor_gain	1.0000
15:42563748:G:GA	acceptor_gain	1.0000
15:42563748:GA:G	acceptor_gain	1.0000
15:42563748:GAT:G	acceptor_gain	1.0000
15:42563748:GATA:G	acceptor_gain	1.0000
15:42563748:GATAT:G	acceptor_gain	1.0000
15:42563853:AAATG:A	donor_gain	1.0000
15:42563854:AATG:A	donor_gain	1.0000
15:42563855:ATG:A	donor_gain	1.0000
15:42563856:TG:T	donor_gain	1.0000
15:42563856:TGG:T	donor_loss	1.0000
15:42563857:GG:G	donor_gain	1.0000
15:42563858:G:C	donor_loss	1.0000
15:42563858:G:GG	donor_gain	1.0000
15:42563859:T:A	donor_loss	1.0000
15:42566596:A:AG	acceptor_gain	1.0000
15:42566596:AAAT:A	acceptor_gain	1.0000
15:42566597:A:G	acceptor_gain	1.0000

AlphaMissense

1564 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
15:42561321:T:C	L103P	0.941
15:42561351:T:C	L113P	0.925
15:42563783:T:C	F142L	0.924
15:42563785:C:A	F142L	0.924
15:42563785:C:G	F142L	0.924
15:42559346:T:C	L65P	0.923
15:42561309:T:C	L99S	0.914
15:42558276:G:C	A58P	0.911
15:42561339:T:C	L109P	0.911
15:42559361:T:C	L70P	0.907
15:42559358:T:C	L69P	0.906
15:42561288:T:C	L92P	0.899
15:42566656:T:C	L183P	0.895
15:42566676:T:A	W190R	0.889
15:42566676:T:C	W190R	0.889
15:42563808:T:C	L150P	0.875
15:42559381:G:C	A77P	0.869
15:42559367:T:C	L72P	0.865
15:42566658:G:C	A184P	0.860
15:42563775:C:A	A139D	0.853
15:42566678:G:C	W190C	0.853
15:42566678:G:T	W190C	0.853
15:42561314:G:C	A101P	0.844
15:42563774:G:C	A139P	0.835
15:42566680:G:C	R191P	0.833
15:42561378:T:C	L122S	0.832
15:42561351:T:A	L113Q	0.818
15:42561318:T:A	V102E	0.816
15:42558234:T:C	F44L	0.813
15:42558236:T:A	F44L	0.813

dbSNP variants (sampled 300 via entrez): RS1000033769 (15:42547765 A>C), RS1000085531 (15:42548182 CTTG>C), RS1000389007 (15:42554227 C>T), RS1000690049 (15:42565399 G>T), RS1000781138 (15:42563947 C>G,T), RS1000895691 (15:42564157 C>T), RS1001126324 (15:42557616 T>G), RS1001341324 (15:42558501 G>A), RS1001412235 (15:42557477 T>A,G), RS1001569294 (15:42565158 A>G), RS1001811949 (15:42546847 A>C,G), RS1001910669 (15:42553480 T>C), RS1001999922 (15:42552258 A>C,T), RS1002033646 (15:42547322 C>T), RS1002413514 (15:42564571 A>G)

Disease associations

OMIM: gene MIM:613429 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

2 associations (top):

Study	Trait	p-value
GCST002726_31	Glucose homeostasis traits	6.000000e-06
GCST008103_17	Bipolar disorder	9.000000e-09

EFO canonical traits (1, from GWAS)

EFO ID	Trait name
EFO:0004471	insulin sensitivity measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

40 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Cisplatin	affects cotreatment, decreases expression, increases expression	2
Hydrogen Peroxide	affects expression	2
afuresertib	decreases expression	1
dicrotophos	decreases expression	1
triphenyl phosphate	affects expression	1
alpha-pinene	affects cotreatment, decreases expression, increases abundance	1
deoxynivalenol	increases expression	1
glycidyl methacrylate	increases expression	1
lead acetate	affects cotreatment, increases expression	1
zinc protoporphyrin	affects cotreatment, increases expression	1
methacrylaldehyde	decreases expression, increases abundance, affects cotreatment	1
di-n-butylphosphoric acid	affects expression	1
K 7174	increases expression	1
torcetrapib	increases expression	1
ICG 001	increases expression	1
jinfukang	affects cotreatment, decreases expression	1
4-(4-((5-(4,5-dimethyl-2-nitrophenyl)-2-furanyl)methylene)-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-1-yl)benzoic acid	increases expression	1
Resveratrol	affects cotreatment, decreases expression, increases expression	1
Sunitinib	decreases expression	1
Acrolein	affects cotreatment, decreases expression, increases abundance	1
Air Pollutants	increases abundance, affects cotreatment, decreases expression	1
Coumestrol	decreases expression	1
Demecolcine	decreases expression	1
Doxorubicin	decreases expression	1
Estradiol	affects expression	1
Formaldehyde	increases expression	1
Lead	increases expression	1
Methyl Methanesulfonate	increases expression	1
Ozone	increases abundance, affects cotreatment, decreases expression	1
Plant Extracts	affects cotreatment, decreases expression, increases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.