HAUS3

Summary

HAUS3 (HAUS augmin like complex subunit 3, HGNC:28719) is a protein-coding gene on chromosome 4p16.3, encoding HAUS augmin-like complex subunit 3 (Q68CZ6). Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. It is a common-essential gene (DepMap: required in 94.3% of cancer cell lines).

This gene encodes a component of the HAUS augmin-like protein complex, which plays a key role in cytokinesis and mitosis. Disruption of the encoded protein causes mitotic defects resulting from fragmentation of centrosomes and microtubule destabilization. This gene shares its 5’ exons with some transcripts from overlapping GeneID: 353497, which encodes a DNA polymerase. Alternative splicing results in multiple transcript variants.

Source: NCBI Gene 79441 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 97 total
• Cancer dependency (DepMap): dependent in 94.3% of screened cell lines (common-essential)
• MANE Select transcript: NM_001303143

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:28719
Approved symbol	HAUS3
Name	HAUS augmin like complex subunit 3
Location	4p16.3
Locus type	gene with protein product
Status	Approved
Aliases	MGC4701, IT1, dgt3
Ensembl gene	ENSG00000214367
Ensembl biotype	protein_coding
OMIM	613430
Entrez	79441

Gene structure

Transcript identifiers

Ensembl transcripts: 5 — 5 protein_coding

ENST00000243706, ENST00000443786, ENST00000502440, ENST00000506763, ENST00000514395

RefSeq mRNA: 2 — MANE Select: NM_001303143 NM_001303143, NM_024511

CCDS: CCDS33941

Canonical transcript exons

ENST00000443786 — 6 exons

Exon	Start	End
ENSE00000843049	2236228	2236456
ENSE00000843050	2238604	2239043
ENSE00000843051	2240038	2241093
ENSE00001388589	2228349	2232160
ENSE00003472748	2241520	2241790
ENSE00003655151	2242051	2242121

Expression profiles

Bgee: expression breadth ubiquitous, 261 present calls, max score 88.27.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 4.0187 / max 44.9892, expressed in 1482 samples.

FANTOM5 promoters (5 alternative TSS)

Promoter ID	TPM avg	Samples expressed
51109	4.0187	1482
51107	2.4718	874
51108	1.5590	922
51106	0.5361	216
51105	0.1876	48

Top tissues by expression

286 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
secondary oocyte	CL:0000655	88.27	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	87.48	gold quality
oocyte	CL:0000023	83.52	gold quality
calcaneal tendon	UBERON:0003701	83.17	gold quality
ventricular zone	UBERON:0003053	83.10	gold quality
oral cavity	UBERON:0000167	82.10	gold quality
visceral pleura	UBERON:0002401	81.76	gold quality
parietal pleura	UBERON:0002400	81.54	gold quality
ganglionic eminence	UBERON:0004023	81.24	gold quality
cortical plate	UBERON:0005343	81.23	gold quality
bone marrow	UBERON:0002371	81.11	gold quality
pleura	UBERON:0000977	80.85	gold quality
upper leg skin	UBERON:0004262	80.37	gold quality
granulocyte	CL:0000094	79.98	gold quality
mucosa of sigmoid colon	UBERON:0004993	79.87	gold quality
body of pancreas	UBERON:0001150	79.86	gold quality
palpebral conjunctiva	UBERON:0001812	79.68	gold quality
skin of hip	UBERON:0001554	79.62	gold quality
leukocyte	CL:0000738	79.22	gold quality
corpus epididymis	UBERON:0004359	79.22	gold quality
adrenal tissue	UBERON:0018303	79.06	gold quality
mononuclear cell	CL:0000842	79.00	gold quality
epithelium of nasopharynx	UBERON:0001951	78.99	gold quality
monocyte	CL:0000576	78.91	gold quality
esophagus squamous epithelium	UBERON:0006920	78.85	gold quality
tibia	UBERON:0000979	78.43	gold quality
jejunal mucosa	UBERON:0000399	78.36	gold quality
pancreas	UBERON:0001264	78.19	gold quality
lymph node	UBERON:0000029	78.16	gold quality
germinal epithelium of ovary	UBERON:0001304	78.01	gold quality

Single-cell (SCXA)

Detected in 5 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	4.85
E-MTAB-6379	no	4061.74
E-GEOD-110499	no	436.92
E-MTAB-7249	no	266.98
E-MTAB-6142	no	153.05

Regulation

Is transcription factor: no

Functional genomics

DepMap (CRISPR cell-line fitness): dependent in 94.3% of screened cell lines, common-essential.

Literature-anchored findings (GeneRIF, showing 2)

• PDK1-stabilized LncRNA SPRY4-IT1 promotes breast cancer progression via activating NF-kappaB signaling pathway. (PMID:37042573)
• RUNX1-IT1 acts as a scaffold of STAT1 and NuRD complex to promote ROS-mediated NF-kappaB activation and ovarian cancer progression. (PMID:38092960)

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
danio_rerio	haus3	ENSDARG00000061385
mus_musculus	Haus3	ENSMUSG00000079555

Protein

Protein identifiers

HAUS augmin-like complex subunit 3 — Q68CZ6 (reviewed: Q68CZ6)

All UniProt accessions (3): A0A1D5RMS3, D6R993, Q68CZ6

UniProt curated annotations — full annotation on UniProt →

Function. Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.

Subunit / interactions. Component of the HAUS augmin-like complex. The complex interacts with the gamma-tubulin ring complex and this interaction is required for spindle assembly. Interacts with EML3 (phosphorylated at ‘Thr-881’).

Subcellular location. Cytoplasm. Cytoskeleton. Microtubule organizing center. Centrosome. Spindle.

Similarity. Belongs to the HAUS3 family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q68CZ6-1	1	yes
Q68CZ6-2	2

RefSeq proteins (2): NP_001290072, NP_078787 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR026206	HAUS3	Family
IPR032733	HAUS3_N	Domain

Pfam: PF14932

UniProt features (10 total): coiled-coil region 4, splice variant 2, initiator methionine 1, chain 1, modified residue 1, sequence variant 1

Structure

Experimental structures (PDB)

3 structures.

PDB	Method	Resolution (Å)
6PTE	X-RAY DIFFRACTION	1.9
6PTB	X-RAY DIFFRACTION	2.15
7SQK	ELECTRON MICROSCOPY	8

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q68CZ6-F1	78.85	0.29

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (1): 2

Function

Pathways and Gene Ontology

Reactome pathways

7 pathways

ID	Pathway
R-HSA-2565942	Regulation of PLK1 Activity at G2/M Transition
R-HSA-380259	Loss of Nlp from mitotic centrosomes
R-HSA-380270	Recruitment of mitotic centrosome proteins and complexes
R-HSA-380284	Loss of proteins required for interphase microtubule organization from the centrosome
R-HSA-380320	Recruitment of NuMA to mitotic centrosomes
R-HSA-5620912	Anchoring of the basal body to the plasma membrane
R-HSA-8854518	AURKA Activation by TPX2

MSigDB gene sets: 214 (showing top): RODRIGUES_THYROID_CARCINOMA_ANAPLASTIC_UP, GOBP_REGULATION_OF_PROTEIN_POLYMERIZATION, GOBP_REGULATION_OF_MICROTUBULE_BASED_PROCESS, PAL_PRMT5_TARGETS_UP, DACOSTA_UV_RESPONSE_VIA_ERCC3_UP, GOBP_MICROTUBULE_NUCLEATION, BROWNE_HCMV_INFECTION_12HR_UP, GOBP_REGULATION_OF_CELLULAR_COMPONENT_BIOGENESIS, GGGTGGRR_PAX4_03, GOCC_MICROTUBULE_ORGANIZING_CENTER, PATIL_LIVER_CANCER, PUJANA_CHEK2_PCC_NETWORK, ONKEN_UVEAL_MELANOMA_UP, DEURIG_T_CELL_PROLYMPHOCYTIC_LEUKEMIA_DN, GOBP_REGULATION_OF_MICROTUBULE_POLYMERIZATION

GO Biological Process (5): centrosome cycle (GO:0007098), regulation of microtubule nucleation (GO:0010968), microtubule organizing center organization (GO:0031023), spindle assembly (GO:0051225), cell division (GO:0051301)

GO Molecular Function (1): protein binding (GO:0005515)

GO Cellular Component (16): nucleoplasm (GO:0005654), mitochondrion (GO:0005739), centrosome (GO:0005813), microtubule organizing center (GO:0005815), cytosol (GO:0005829), cilium (GO:0005929), microtubule cytoskeleton (GO:0015630), ciliary basal body (GO:0036064), intercellular bridge (GO:0045171), HAUS complex (GO:0070652), mitotic spindle (GO:0072686), mitotic spindle microtubule (GO:1990498), cytoplasm (GO:0005737), spindle (GO:0005819), cytoskeleton (GO:0005856), microtubule (GO:0005874)

Reactome top-level categories

Rollup of top-5 pathways:

Category	Pathways
G2/M Transition	2
Centrosome maturation	2
Loss of proteins required for interphase microtubule organization from the centrosome	1
Mitotic Prometaphase	1
Assembly of the 9+0 primary cilium	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	5
microtubule cytoskeleton	3
cytoplasm	2
microtubule organizing center	2
intracellular membraneless organelle	2
cell cycle process	1
microtubule organizing center organization	1
microtubule nucleation	1
regulation of microtubule polymerization	1
microtubule cytoskeleton organization	1
microtubule-based process	1
cellular component organization	1
spindle organization	1
chromosome segregation	1
membraneless organelle assembly	1
cellular process	1
binding	1
nuclear lumen	1
intracellular membrane-bounded organelle	1
centriole	1
intraciliary transport particle	1
membrane-bounded organelle	1
plasma membrane bounded cell projection	1
cytoskeleton	1
cilium	1
microtubule associated complex	1
spindle	1
spindle microtubule	1
mitotic spindle	1
intracellular anatomical structure	1
polymeric cytoskeletal fiber	1

Protein interactions and networks

STRING

2242 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
HAUS3	HAUS6	Q7Z4H7	971
HAUS3	HAUS5	O94927	854
HAUS3	HAUS4	Q9H6D7	827
HAUS3	HAUS8	Q9BT25	824
HAUS3	HAUS7	Q99871	781
HAUS3	NUMA1	Q14980	709
HAUS3	NEDD1	Q8NHV4	706
HAUS3	TUBG1	P23258	693
HAUS3	HAUS2	Q9NVX0	682
HAUS3	HAUS1	Q96CS2	667
HAUS3	HELQ	Q8TDG4	532
HAUS3	RELCH	Q9P260	512
HAUS3	TAPT1	Q6NXT6	487
HAUS3	NSUN7	Q8NE18	457
HAUS3	POLN	Q7Z5Q5	453

IntAct

159 interactions, top by confidence:

A	B	Type	Score
MED4	MED19	psi-mi:“MI:2364”(proximity)	0.900
CEP290	CCP110	psi-mi:“MI:2364”(proximity)	0.890
HAUS1	HAUS5	psi-mi:“MI:0915”(physical association)	0.780
HAUS1	HAUS5	psi-mi:“MI:0914”(association)	0.780
HAUS2	HAUS5	psi-mi:“MI:0914”(association)	0.730
TXLNA	HAUS3	psi-mi:“MI:0915”(physical association)	0.720
HAUS3	TXLNA	psi-mi:“MI:0915”(physical association)	0.720
HAUS6	HAUS3	psi-mi:“MI:0915”(physical association)	0.710
HAUS6	HAUS5	psi-mi:“MI:0914”(association)	0.710
LUZP1	DAPK3	psi-mi:“MI:0914”(association)	0.670
CEP170	KIF2A	psi-mi:“MI:2364”(proximity)	0.650
COG3	COG7	psi-mi:“MI:0914”(association)	0.640
HAUS4	HAUS5	psi-mi:“MI:0914”(association)	0.640
BRME1	HAUS3	psi-mi:“MI:0915”(physical association)	0.560
TXLNB	HAUS3	psi-mi:“MI:0915”(physical association)	0.560
HAUS3	BRME1	psi-mi:“MI:0915”(physical association)	0.560
Haus4	HAUS5	psi-mi:“MI:0914”(association)	0.560
HAUS3	GNG13	psi-mi:“MI:0915”(physical association)	0.560
CIAO1	HAUS3	psi-mi:“MI:0915”(physical association)	0.560
HAUS3	ANKRD11	psi-mi:“MI:0915”(physical association)	0.560
HAUS3	RCOR3	psi-mi:“MI:0915”(physical association)	0.560

BioGRID (149): HAUS3 (Two-hybrid), TXLNB (Two-hybrid), TXLNA (Two-hybrid), HAUS3 (Affinity Capture-MS), HAUS3 (Affinity Capture-MS), HAUS3 (Proximity Label-MS), HAUS3 (Proximity Label-MS), HAUS3 (Proximity Label-MS), HAUS3 (Proximity Label-MS), HAUS3 (Proximity Label-MS), HAUS3 (Proximity Label-MS), HAUS3 (Proximity Label-MS), HAUS3 (Proximity Label-MS), HAUS3 (Proximity Label-MS), HAUS3 (Proximity Label-MS)

ESM2 similar proteins: A0JMY4, A2AJB1, A3KQH2, A7RNG8, B1H228, E9Q9F7, F1N2N9, F1RKB1, M1V4Y8, Q0VFX2, Q16VW9, Q17QH9, Q28G12, Q2TA00, Q2TAA8, Q3KPZ2, Q3SZX9, Q3T0L1, Q3USS3, Q3UX62, Q3UZ57, Q4R6I5, Q4R8Y5, Q4V7B0, Q502W7, Q5T1B0, Q5T5S1, Q5XIR6, Q5XIR8, Q66H60, Q68CZ6, Q6DCY9, Q6DHI2, Q6PA15, Q8BSN3, Q8C5T8, Q8CDN8, Q8CDV6, Q8IWF9, Q8NA47

Diamond homologs: Q68CZ6, Q6DCY9, Q8QZX2

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
HAUS3	“form complex”	“HAUS complex”	binding

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 159 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Loss of Nlp from mitotic centrosomes	19	33.1×	2e-22
Loss of proteins required for interphase microtubule organization from the centrosome	19	33.1×	2e-22
AURKA Activation by TPX2	19	31.8×	4e-22
Anchoring of the basal body to the plasma membrane	23	28.6×	2e-25
Recruitment of mitotic centrosome proteins and complexes	19	28.4×	4e-21
Regulation of PLK1 Activity at G2/M Transition	19	26.5×	1e-20
Recruitment of NuMA to mitotic centrosomes	19	24.3×	6e-20
Centrosome maturation	5	13.9×	1e-03

GO biological processes:

GO term	Partners	Fold	FDR
centriole replication	8	42.5×	2e-09
centrosome cycle	12	29.3×	4e-12
spindle assembly	8	25.7×	1e-07
non-motile cilium assembly	7	14.7×	5e-05
mitotic spindle assembly	5	12.5×	3e-03
cilium assembly	14	7.5×	7e-07
cell division	20	6.7×	3e-09

Disease & clinical

Clinical variants and AI predictions

ClinVar

97 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	90
Likely benign	1
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1190 predictions. Top by Δscore:

Variant	Effect	Δscore
4:2231943:ACAG:A	donor_gain	1.0000
4:2231944:CAGC:C	donor_gain	1.0000
4:2232033:T:A	donor_gain	1.0000
4:2236223:CCCA:C	donor_loss	1.0000
4:2236224:CCAC:C	donor_loss	1.0000
4:2236226:A:T	donor_loss	1.0000
4:2236227:C:G	donor_loss	1.0000
4:2236455:GC:G	acceptor_gain	1.0000
4:2236456:CC:C	acceptor_gain	1.0000
4:2236457:C:CA	acceptor_loss	1.0000
4:2236457:C:CC	acceptor_gain	1.0000
4:2236458:T:A	acceptor_loss	1.0000
4:2238602:A:AC	donor_gain	1.0000
4:2238603:C:CC	donor_gain	1.0000
4:2239040:CAGC:C	acceptor_gain	1.0000
4:2239042:GCC:G	acceptor_loss	1.0000
4:2239043:CCTAT:C	acceptor_loss	1.0000
4:2239044:C:CC	acceptor_gain	1.0000
4:2239044:CTAT:C	acceptor_loss	1.0000
4:2240033:CTTA:C	donor_loss	1.0000
4:2240034:TTA:T	donor_loss	1.0000
4:2240035:TA:T	donor_loss	1.0000
4:2240036:ACCT:A	donor_loss	1.0000
4:2240037:C:A	donor_loss	1.0000
4:2229098:CT:C	donor_gain	0.9900
4:2229098:CTCT:C	donor_gain	0.9900
4:2231944:CAG:C	donor_gain	0.9900
4:2232158:CTC:C	acceptor_gain	0.9900
4:2232159:TCC:T	acceptor_loss	0.9900
4:2232161:C:G	acceptor_loss	0.9900

AlphaMissense

4025 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
4:2240862:A:G	W29R	0.961
4:2240862:A:T	W29R	0.961
4:2238923:C:G	A344P	0.959
4:2240153:A:G	L265P	0.958
4:2238809:C:G	A382P	0.954
4:2238796:A:G	L386P	0.952
4:2240823:A:G	W42R	0.950
4:2240823:A:T	W42R	0.950
4:2240281:G:C	F222L	0.941
4:2240281:G:T	F222L	0.941
4:2240283:A:G	F222L	0.941
4:2240588:C:G	R120P	0.937
4:2238823:A:G	L377S	0.933
4:2240819:A:G	F43S	0.932
4:2240591:C:G	R119P	0.929
4:2240927:A:G	F7S	0.929
4:2238813:T:A	Q380H	0.927
4:2238813:T:G	Q380H	0.927
4:2238843:T:A	Q370H	0.925
4:2238843:T:G	Q370H	0.925
4:2240854:A:C	F31L	0.921
4:2240854:A:T	F31L	0.921
4:2240856:A:G	F31L	0.921
4:2238872:C:G	A361P	0.914
4:2240866:A:C	F27L	0.912
4:2240866:A:T	F27L	0.912
4:2240868:A:G	F27L	0.912
4:2238835:A:T	V373D	0.905
4:2238880:A:G	L358P	0.905
4:2240860:C:A	W29C	0.904

dbSNP variants (sampled 300 via entrez): RS1000123561 (4:2232670 TAC>T), RS1000495893 (4:2232860 CAA>C), RS1000535985 (4:2228676 C>T), RS1000991345 (4:2242658 A>C), RS1001277229 (4:2243724 G>A), RS1001320293 (4:2242425 G>A), RS1001410288 (4:2237675 T>C), RS1001487087 (4:2240721 C>A,T), RS1001536114 (4:2232170 G>A), RS1001567118 (4:2233843 G>C), RS1001915491 (4:2233342 G>A), RS1001915797 (4:2241848 C>T), RS1002037598 (4:2227984 A>G), RS1002302563 (4:2235131 G>A,T), RS1002307537 (4:2229553 G>A)

Disease associations

OMIM: gene MIM:613430 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

25 total (human), top 25 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Valproic Acid	decreases expression, decreases methylation	3
Cyclosporine	decreases expression, increases expression	2
3-((6-(2-methoxyphenyl)pyrimidin-4-yl)amino)phenyl)methane sulfonamide	decreases expression	1
triphenyl phosphate	affects expression	1
bisphenol A	affects cotreatment, decreases methylation	1
beta-lapachone	increases expression	1
sodium arsenite	decreases expression	1
pentanal	decreases expression	1
K 7174	increases expression	1
Fulvestrant	affects cotreatment, decreases methylation	1
Acetaminophen	increases expression	1
Aldehydes	decreases expression	1
Arsenic	affects expression	1
Dimethyl Sulfoxide	increases expression	1
Estradiol	affects expression	1
Quercetin	decreases expression	1
Rotenone	decreases expression	1
Selenium	affects cotreatment, increases expression, decreases expression	1
Urethane	increases expression	1
Vitamin E	affects cotreatment, increases expression, decreases expression	1
7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide	increases expression	1
Oxyquinoline	decreases expression	1
Aflatoxin B1	affects expression	1
Sodium Selenite	increases expression	1
Copper Sulfate	decreases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.