HSD17B14

gene
On this page

Also known as retSDR3SDR47C1

Summary

HSD17B14 (hydroxysteroid 17-beta dehydrogenase 14, HGNC:23238) is a protein-coding gene on chromosome 19q13.33, encoding L-fucose dehydrogenase (Q9BPX1). Catalyzes the NAD(+)-dependent oxidation of L-fucose, yielding L-fucono-1,5-lactone, which rapidly converts spontaneously to L-fucone-1,4-lactone.

17-beta-hydroxysteroid dehydrogenases, such as HSD17B14, are primarily involved in metabolism of steroids at the C17 position and also of other substrates, such as fatty acids, prostaglandins, and xenobiotics (Lukacik et al., 2007 [PubMed 17067289]).

Source: NCBI Gene 51171 — RefSeq curated summary.

At a glance

  • GWAS associations: 2
  • Clinical variants (ClinVar): 52 total
  • Druggable target: yes
  • MANE Select transcript: NM_016246

Identifiers

Gene identifiers

FieldValue
HGNC IDHGNC:23238
Approved symbolHSD17B14
Namehydroxysteroid 17-beta dehydrogenase 14
Location19q13.33
Locus typegene with protein product
StatusApproved
AliasesretSDR3, SDR47C1
Ensembl geneENSG00000087076
Ensembl biotypeprotein_coding
OMIM612832
Entrez51171

Gene structure

Transcript identifiers

Ensembl transcripts: 13 — 13 protein_coding

ENST00000263278, ENST00000595764, ENST00000596349, ENST00000599157, ENST00000867477, ENST00000867478, ENST00000867479, ENST00000867480, ENST00000867481, ENST00000867482, ENST00000867483, ENST00000931891, ENST00000931892

RefSeq mRNA: 1 — MANE Select: NM_016246 NM_016246

CCDS: CCDS12736

Canonical transcript exons

ENST00000263278 — 9 exons

ExonStartEnd
ENSE000005548724883166848831759
ENSE000007182414881345648813552
ENSE000007182474881366348813730
ENSE000007182534881503748815141
ENSE000007182644883266648832732
ENSE000007182684883427648834358
ENSE000007182744883580548835843
ENSE000012351204883632448836491
ENSE000031175354881301848813348

Expression profiles

Bgee: expression breadth ubiquitous, 227 present calls, max score 94.48.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 8.6333 / max 140.5108, expressed in 1311 samples.

FANTOM5 promoters (4 alternative TSS)

Promoter IDTPM avgSamples expressed
1819385.74181119
1819361.5152570
1819371.0011511
1819390.3751217

Top tissues by expression

280 total, by Bgee expression score (0-100, higher = more expressed):

TissueAnatomy IDExpression scoreQuality
mucosa of stomachUBERON:000119994.48gold quality
right adrenal gland cortexUBERON:003582794.22gold quality
right lobe of liverUBERON:000111493.70gold quality
left ovaryUBERON:000211993.62gold quality
left adrenal gland cortexUBERON:003582592.54gold quality
adrenal cortexUBERON:000123592.22gold quality
left adrenal glandUBERON:000123492.20gold quality
right adrenal glandUBERON:000123392.11gold quality
nucleus accumbensUBERON:000188291.85gold quality
male germ line stem cell (sensu Vertebrata) in testisCL:0000089 ∩ UBERON:000047391.24gold quality
right ovaryUBERON:000211891.21gold quality
right hemisphere of cerebellumUBERON:001489091.16gold quality
caudate nucleusUBERON:000187391.07gold quality
amygdalaUBERON:000187690.75gold quality
cerebellar hemisphereUBERON:000224590.68gold quality
cerebellar cortexUBERON:000212990.59gold quality
endocervixUBERON:000045890.42gold quality
putamenUBERON:000187489.98gold quality
adult mammalian kidneyUBERON:000008289.89gold quality
body of uterusUBERON:000985389.74gold quality
left uterine tubeUBERON:000130389.52gold quality
anterior cingulate cortexUBERON:000983589.51gold quality
cingulate cortexUBERON:000302789.45gold quality
adrenal glandUBERON:000236989.34gold quality
right frontal lobeUBERON:000281089.11gold quality
pigmented layer of retinaUBERON:000178289.03gold quality
retinaUBERON:000096689.01gold quality
cerebellumUBERON:000203789.00gold quality
adenohypophysisUBERON:000219688.93gold quality
left lobe of thyroid glandUBERON:000112088.78gold quality

Single-cell (SCXA)

Detected in 3 experiment(s), a significant marker in 3.

ExperimentMarker?Max mean expression
E-MTAB-6701yes45.19
E-MTAB-5061yes25.94
E-ANND-3no0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

14 targeting HSD17B14, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNAMax scoreAvg scoremiRNA target_count
HSA-MIR-378G99.7164.901106
HSA-MIR-569799.3967.741249
HSA-MIR-3064-5P99.2666.131497
HSA-MIR-3085-3P99.2666.161490
HSA-MIR-6504-5P99.2665.951487
HSA-MIR-465199.0667.572002
HSA-MIR-60898.9367.832013
HSA-MIR-6829-5P98.8665.121480
HSA-MIR-6819-5P97.9666.591071
HSA-MIR-5007-5P97.9564.71614
HSA-MIR-6737-5P97.7566.541044
HSA-MIR-296-5P97.6164.02851
HSA-MIR-6812-5P97.5665.391059
HSA-MIR-874-5P96.9363.921014

Literature-anchored findings (GeneRIF, showing 5)

  • crystal structure of the DHRS10 apoenzyme exhibits secondary structure of the SDR (short-chain dehydrogenase/reductase) family (PMID:17067289)
  • Results show that tumoural expression levels of 17betaHSD14 can predict the outcome of adjuvant tamoxifen treatment in terms of local recurrence-free survival in patients with lymph node-negative ER+ breast cancer. (PMID:22792371)
  • Five new variants of HSD17B14 characterized by crystallography and enzyme kinetics. Tyr253’ from the adjacent monomer ties the two monomers together. Disulfide bridge formed by Cys255 and Cys255’ is not crucial for dimer stabilization. (PMID:30836176)
  • Association of Coding Variants in Hydroxysteroid 17-beta Dehydrogenase 14 (HSD17B14) with Reduced Progression to End Stage Kidney Disease in Type 1 Diabetes. (PMID:34261756)
  • Hydroxysteroid 17-beta dehydrogenase 14 (HSD17B14) is an L-fucose dehydrogenase, the initial enzyme of the L-fucose degradation pathway. (PMID:38944119)

Cross-species orthologs

4 orthologs

OrganismSymbolGene ID
danio_reriohsd17b14ENSDARG00000054842
mus_musculusHsd17b14ENSMUSG00000030825
rattus_norvegicusHsd17b14ENSRNOG00000020949
caenorhabditis_elegansWBGENE00014093

Paralogs (25): HSD17B6 (ENSG00000025423), RDH11 (ENSG00000072042), HSD17B10 (ENSG00000072506), DHRS9 (ENSG00000073737), HSD17B2 (ENSG00000086696), DHRS12 (ENSG00000102796), HSDL1 (ENSG00000103160), HSD17B1 (ENSG00000108786), RDH10 (ENSG00000121039), HSD17B3 (ENSG00000130948), HSD17B7 (ENSG00000132196), HSD17B4 (ENSG00000133835), RDH5 (ENSG00000135437), RDH16 (ENSG00000139547), RDH12 (ENSG00000139988), HSD17B12 (ENSG00000149084), BDH1 (ENSG00000161267), DHRS3 (ENSG00000162496), SDR9C7 (ENSG00000170426), HSD17B13 (ENSG00000170509), SDR16C5 (ENSG00000170786), HSD11B2 (ENSG00000176387), WWOX (ENSG00000186153), HSD17B11 (ENSG00000198189), HSD17B8 (ENSG00000204228)

Protein

Protein identifiers

L-fucose dehydrogenaseQ9BPX1 (reviewed: Q9BPX1)

Alternative names: 17-beta-hydroxysteroid dehydrogenase DHRS10, Dehydrogenase/reductase SDR family member 10, Retinal short-chain dehydrogenase/reductase retSDR3, Short chain dehydrogenase/reductase family 47C member 1

All UniProt accessions (5): Q9BPX1, A0A140VJH8, M0QY35, M0R147, M0R2R3

UniProt curated annotations — full annotation on UniProt →

Function. Catalyzes the NAD(+)-dependent oxidation of L-fucose, yielding L-fucono-1,5-lactone, which rapidly converts spontaneously to L-fucone-1,4-lactone. Can also act on D-arabinose and L-galactose, with lower catalytic efficiency. Does not use NADPH. May be the initial enzyme of the L-fucose degradation pathway in mammals.

Subunit / interactions. Homotetramer.

Subcellular location. Cytoplasm.

Tissue specificity. Highly expressed in brain, placenta, liver and kidney.

Pathway. Carbohydrate degradation; L-fucose degradation.

Similarity. Belongs to the short-chain dehydrogenases/reductases (SDR) family.

RefSeq proteins (1): NP_057330* (*=MANE)

Domains & families (InterPro)

IDNameType
IPR002347SDR_famFamily
IPR020904Sc_DH/Rdtase_CSConserved_site
IPR036291NAD(P)-bd_dom_sfHomologous_superfamily

Pfam: PF13561

Enzyme classification (BRENDA):

  • EC 1.1.1.62 — 17beta-estradiol 17-dehydrogenase (BRENDA: 20 organisms, 283 substrates, 790 inhibitors, 95 Km, 44 kcat entries)

Substrate kinetics (BRENDA)

32 substrates with measured Km, best-characterized 15. Km ranges are aggregated across organisms/conditions.

SubstrateKm (mM)Measurements
ESTRADIOL-17BETA0.0008–0.02514
ESTRONE10
NADP+0.0001–99
17BETA-ESTRADIOL0.0006–0.0827
NADPH0.0003–0.167
ESTRADIOL0.0036–0.1186
TESTOSTERONE0.0071–0.2634
DEHYDROEPIANDROSTERONE0.0172–0.05983
5-ANDROSTENE-3BETA,17BETA-DIOL0.0066–0.00782
5ALPHA-DIHYDROTESTOSTERONE0.0067–0.1182
5BETA-PREGNAN-20ALPHA-OL-3-ONE0.0011–0.00332
DIHYDROTESTOSTERONE0.0043–0.0332
NAD+0.357–0.52
(S)-INDAN-1-OL0.5081
1-METHYL-6-DEHYDROESTRADIOL0.00851

Catalyzed reactions (Rhea), 3 shown:

  • L-fucose + NAD(+) = L-fucono-1,5-lactone + NADH + H(+) (RHEA:81515)
  • D-arabinose + NAD(+) = D-arabinono-1,5-lactone + NADH + H(+) (RHEA:81519)
  • L-galactose + NAD(+) = L-galactono-1,5-lactone + NADH + H(+) (RHEA:81523)

UniProt features (45 total): binding site 12, helix 12, strand 8, mutagenesis site 5, turn 3, sequence variant 2, chain 1, active site 1, sequence conflict 1

Structure

Experimental structures (PDB)

31 structures, top 30 by resolution.

PDBMethodResolution (Å)
6H0MX-RAY DIFFRACTION1.25
5O6XX-RAY DIFFRACTION1.35
6G4LX-RAY DIFFRACTION1.44
5O6OX-RAY DIFFRACTION1.45
5O43X-RAY DIFFRACTION1.5
6ZR6X-RAY DIFFRACTION1.5
5EN4X-RAY DIFFRACTION1.52
5ICSX-RAY DIFFRACTION1.52
5O6ZX-RAY DIFFRACTION1.57
5O7CX-RAY DIFFRACTION1.6
6GTBX-RAY DIFFRACTION1.62
6FFBX-RAY DIFFRACTION1.65
5ICMX-RAY DIFFRACTION1.68
6HNOX-RAY DIFFRACTION1.68
6QCKX-RAY DIFFRACTION1.68
6ZRAX-RAY DIFFRACTION1.73
5L7WX-RAY DIFFRACTION1.76
5O42X-RAY DIFFRACTION1.76
5JSFX-RAY DIFFRACTION1.84
6ZDEX-RAY DIFFRACTION1.87
5O72X-RAY DIFFRACTION1.91
5L7YX-RAY DIFFRACTION1.91
6ZT2X-RAY DIFFRACTION1.95
5L7TX-RAY DIFFRACTION1.98
5JS6X-RAY DIFFRACTION2
5HS6X-RAY DIFFRACTION2.02
6GBTX-RAY DIFFRACTION2.1
6ZDIX-RAY DIFFRACTION2.13
6GTUX-RAY DIFFRACTION2.25
1YDEX-RAY DIFFRACTION2.4

Predicted structure (AlphaFold)

ModelpLDDTFraction very-high
AF-Q9BPX1-F196.670.94

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Catalytic / active sites (1): 154 (proton acceptor)

Ligand- & substrate-binding residues (12): 158; 187; 189; 191; 19; 21; 40; 41; 62; 63; 89; 154

Mutagenesis-validated functional residues (5):

PositionPhenotype
93increases kcat for androst-5-en-3beta,17beta-diol and 17beta-estradioll.
148the catalytic efficiency (kcat/km) is 30-fold increase for 17beta-estradiol and 11-fold for androst-5-en-3beta,17beta-di
158lacks of activity of testosterone 17-beta-dehydrogenase (nadp+) and estradiol 17-beta-dehydrogenase [nad(p)+] activities
253lacks of activity of testosterone 17-beta-dehydrogenase (nadp+) and estradiol 17-beta-dehydrogenase [nad(p)+] activities
255does not affect kcat for androst-5-en-3beta,17beta-diol and 17beta-estradiol.

Function

Pathways and Gene Ontology

Reactome pathways

1 pathways

IDPathway
R-HSA-193144Estrogen biosynthesis

MSigDB gene sets: 88 (showing top): ACEVEDO_NORMAL_TISSUE_ADJACENT_TO_LIVER_TUMOR_DN, GOBP_MONOSACCHARIDE_CATABOLIC_PROCESS, CHANDRAN_METASTASIS_DN, KOYAMA_SEMA3B_TARGETS_UP, GOBP_CARBOHYDRATE_METABOLIC_PROCESS, NELSON_RESPONSE_TO_ANDROGEN_UP, GOBP_LIPID_METABOLIC_PROCESS, GOBP_SMALL_MOLECULE_CATABOLIC_PROCESS, AACTTT_UNKNOWN, GOBP_CARBOHYDRATE_CATABOLIC_PROCESS, GOBP_FUCOSE_METABOLIC_PROCESS, GOMF_OXIDOREDUCTASE_ACTIVITY_ACTING_ON_CH_OH_GROUP_OF_DONORS, GOBP_MONOSACCHARIDE_METABOLIC_PROCESS, GOBP_STEROID_METABOLIC_PROCESS, MIKKELSEN_IPS_LCP_WITH_H3K4ME3

GO Biological Process (4): steroid metabolic process (GO:0008202), L-fucose catabolic process (GO:0042355), fucose metabolic process (GO:0006004), lipid metabolic process (GO:0006629)

GO Molecular Function (5): identical protein binding (GO:0042802), D-threo-aldose 1-dehydrogenase activity (GO:0047834), estradiol 17-beta-dehydrogenase [NAD(P)+] activity (GO:0004303), protein binding (GO:0005515), oxidoreductase activity (GO:0016491)

GO Cellular Component (2): cytosol (GO:0005829), cytoplasm (GO:0005737)

Reactome top-level categories

Rollup of top-1 pathways:

CategoryPathways
Metabolism of steroid hormones1

GO top-level categories

Rollup of top GO terms by namespace:

CategoryTerms
cellular anatomical structure2
lipid metabolic process1
hexose catabolic process1
L-fucose metabolic process1
hexose metabolic process1
primary metabolic process1
protein binding1
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor1
steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor1
binding1
catalytic activity1
cytoplasm1
intracellular anatomical structure1

Protein interactions and networks

STRING

3281 interactions, top by confidence (×1000):

Protein AProtein BPartner UniProtScore
HSD17B14HSD17B1P14061599
HSD17B14HSD17B3P37058583
HSD17B14HSD17B12Q53GQ0538
HSD17B14FRRS1Q6ZNA5507
HSD17B14AKR1C3P42330445
HSD17B14HSD17B4P51659411
HSD17B14TNFSF12O43508400
HSD17B14ITPRID2P28290397
HSD17B14TNFSF11O14788394
HSD17B14MIOXQ9UGB7391
HSD17B14PLEKHA4Q9H4M7388
HSD17B14DHDHQ9UQ10387
HSD17B14GPER1Q99527387
HSD17B14HSD3B1P14060377
HSD17B14ECHDC1Q9NTX5366

IntAct

149 interactions, top by confidence:

ABTypeScore
NTAQ1HSD17B14psi-mi:“MI:0915”(physical association)0.920
HSD17B14NTAQ1psi-mi:“MI:0915”(physical association)0.920
HSD17B14CA8psi-mi:“MI:0915”(physical association)0.890
CA8HSD17B14psi-mi:“MI:0915”(physical association)0.890
HSD17B14HSD17B14psi-mi:“MI:0915”(physical association)0.860
HSD17B14CDKN2Dpsi-mi:“MI:0915”(physical association)0.790

BioGRID (75): HSD17B14 (Two-hybrid), HSD17B14 (Two-hybrid), HSD17B14 (Two-hybrid), HSD17B14 (Two-hybrid), HSD17B14 (Two-hybrid), HSD17B14 (Two-hybrid), HSD17B14 (Two-hybrid), HSD17B14 (Two-hybrid), HSD17B14 (Two-hybrid), WDYHV1 (Two-hybrid), TBC1D22B (Two-hybrid), LINC00152 (Two-hybrid), SREK1IP1 (Two-hybrid), MIR4435-1HG (Two-hybrid), HSD17B14 (Two-hybrid)

ESM2 similar proteins: A0A097ZPC9, A0A0F7U1Z1, A0A0H3KNE7, A0A1E1FFP5, A0A1L9WLH9, A0A1Y0BRF8, A0A2I1BSW8, A0A2I1C3T5, A0A3G9HAL8, A0A4P8DJW8, A0A6S6QNE4, A0A7T8F1N2, A0A8D5M6H6, A5W4G5, A7AZH2, B5Z114, B6HV34, C8WGQ3, C8WJW0, H1VN83, M1W270, O52384, O69264, P08088, P08694, P0AET8, P0AET9, P0C622, P13859, P19871, P23102, P47227, P50206, P55435, P72220, P9WEQ5, P9WGQ4, P9WGQ5, P9WGQ8, P9WGQ9

Diamond homologs: A0A075TRB3, A0A1L5BU05, A0A2U8U2K8, A0A8F5XX49, A1CFM1, A4FUZ6, C1DMX5, D4Z260, D7PI11, E0D7H5, E9Q3D4, F4JZN6, H1VN83, H9XP47, O24990, O31680, P06235, P07914, P08694, P0A2D1, P0A2D2, P0AG84, P0AG85, P10528, P19337, P39333, P39483, P39484, P39485, P39640, P40398, P42556, P54616, P54795, P9WGQ4, P9WGQ5, Q05069, Q0WRJ2, Q1NEJ0, Q2FZQ3

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

52 variants total. Per-class counts are floors (≥ shown; pagination cap):

ClassificationCount (floor)
Pathogenic0
Likely pathogenic0
Uncertain significance36
Likely benign2
Benign0

Top pathogenic / likely-pathogenic (0)

SpliceAI

1129 predictions. Top by Δscore:

VariantEffectΔscore
19:48813344:AGTGG:Aacceptor_gain1.0000
19:48813345:GTGG:Gacceptor_gain1.0000
19:48813346:TGG:Tacceptor_gain1.0000
19:48813347:GG:Gacceptor_gain1.0000
19:48813348:GC:Gacceptor_loss1.0000
19:48813349:C:CCacceptor_gain1.0000
19:48813349:CTGGG:Cacceptor_loss1.0000
19:48813550:ATA:Aacceptor_gain1.0000
19:48813551:TA:Tacceptor_gain1.0000
19:48813553:C:CCacceptor_gain1.0000
19:48813656:AGCTC:Adonor_loss1.0000
19:48813657:GCTCA:Gdonor_loss1.0000
19:48813658:CTCA:Cdonor_loss1.0000
19:48813659:TCA:Tdonor_loss1.0000
19:48813660:CACCA:Cdonor_loss1.0000
19:48813661:A:ACdonor_gain1.0000
19:48813661:A:AGdonor_loss1.0000
19:48813662:C:CAdonor_loss1.0000
19:48813662:C:CCdonor_gain1.0000
19:48813671:TCGGA:Tdonor_gain1.0000
19:48813672:CGGAC:Cdonor_gain1.0000
19:48813727:CCCC:Cacceptor_gain1.0000
19:48813728:CCC:Cacceptor_gain1.0000
19:48813728:CCCC:Cacceptor_gain1.0000
19:48813729:CC:Cacceptor_gain1.0000
19:48813729:CCC:Cacceptor_gain1.0000
19:48813729:CCCT:Cacceptor_loss1.0000
19:48813730:CC:Cacceptor_gain1.0000
19:48813731:C:CCacceptor_gain1.0000
19:48813731:C:CGacceptor_loss1.0000

AlphaMissense

1714 scored. Top likely-pathogenic:

VariantProtein changeam_pathogenicity
19:48813665:G:CN180K0.992
19:48813665:G:TN180K0.992
19:48815037:C:AK158N0.990
19:48815037:C:GK158N0.990
19:48815088:G:CS141R0.988
19:48815088:G:TS141R0.988
19:48815090:T:GS141R0.988
19:48832679:G:CN88K0.986
19:48832679:G:TN88K0.986
19:48813550:A:TI182N0.985
19:48815097:G:CN138K0.985
19:48815097:G:TN138K0.985
19:48813550:A:CI182S0.977
19:48813550:A:GI182T0.977
19:48813298:G:CF230L0.976
19:48813298:G:TF230L0.976
19:48813300:A:GF230L0.976
19:48813552:A:CC181W0.976
19:48813675:A:TV177D0.974
19:48813711:G:AT165I0.973
19:48815044:G:TA156D0.973
19:48815089:C:AS141I0.973
19:48813315:C:AG225W0.972
19:48815051:A:GY154H0.972
19:48813277:G:CF237L0.971
19:48813277:G:TF237L0.971
19:48813279:A:GF237L0.971
19:48813707:T:AK166N0.971
19:48813707:T:GK166N0.971
19:48813548:A:GS183P0.968

dbSNP variants (sampled 300 via entrez): RS1000041482 (19:48817233 A>G), RS1000051435 (19:48821165 A>T), RS1000109350 (19:48828197 C>T), RS1000413451 (19:48827262 C>T), RS1000431597 (19:48823034 G>A), RS1000755666 (19:48838285 A>C), RS1000794439 (19:48821337 A>G), RS1001102979 (19:48837840 G>A), RS1001325871 (19:48832442 C>T), RS1001350273 (19:48814953 G>A,C), RS1001706161 (19:48821924 T>C), RS1002017154 (19:48814741 G>A), RS1002123819 (19:48837322 A>C,G), RS1002150582 (19:48820977 C>G), RS1002241311 (19:48837689 C>T)

Disease associations

OMIM: gene MIM:612832 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

2 associations (top):

StudyTraitp-value
GCST004523_4Resting metabolic rate3.000000e-06
GCST006585_2580Blood protein levels3.000000e-08

EFO canonical traits (1, from GWAS)

EFO IDTrait name
EFO:0008004resting metabolic rate measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL3712868 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

ChEMBL bioactivities

49 potent at pChembl≥5 of 49 total, top 47 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChemblTypeValueUnitMolecule
8.82Ki1.5nMCHEMBL4175585
8.22Ki6nMCHEMBL4165659
8.22Ki6nMCHEMBL4176263
8.22Ki6nMCHEMBL4164239
8.15Ki7nMCHEMBL3827618
8.15Ki7nMCHEMBL3894507
8.15Ki7nMCHEMBL3965905
8.05Ki9nMCHEMBL3941236
8.05Ki9nMCHEMBL3932068
8.05Ki9nMCHEMBL4168004
8.00Ki10nMCHEMBL4172463
7.96Ki11nMCHEMBL3913249
7.92Ki12nMCHEMBL4171902
7.89Ki13nMCHEMBL3949996
7.82Ki15nMCHEMBL3938843
7.77Ki17nMCHEMBL3911402
7.68Ki21nMCHEMBL3923116
7.64Ki23nMCHEMBL4174579
7.62Ki24nMCHEMBL1928186
7.58Ki26nMCHEMBL1928172
7.47Ki34nMCHEMBL4164576
7.44Ki36nMCHEMBL3922253
7.38Ki42nMCHEMBL4169325
7.36Ki44nMCHEMBL3962753
7.33Ki47nMCHEMBL3954708
7.33Ki47nMCHEMBL4177499
7.30Ki50nMCHEMBL3895144
7.24Ki58nMCHEMBL4171183
7.20Ki63nMCHEMBL3974385
7.19Ki64nMCHEMBL3904245
7.07Ki86nMCHEMBL3968387
7.01Ki97nMCHEMBL3964826
6.92Ki119nMCHEMBL4175870
6.88Ki132nMCHEMBL3904073
6.87Ki135nMCHEMBL3932242
6.72Ki190nMCHEMBL3946020
6.72Ki190nMCHEMBL3914131
6.66Ki221nMCHEMBL3975350
6.61Ki245nMCHEMBL1928188
6.47Ki336nMCHEMBL4160081
6.39Ki405nMCHEMBL3924502
6.39Ki407nMCHEMBL3983947
6.33Ki467nMCHEMBL1928057
6.16Ki686nMCHEMBL4167395
6.10Ki796nMCHEMBL3923295
5.81Ki1541nMCHEMBL3975921
5.69Ki2030nMCHEMBL4170719

PubChem BioAssay actives

49 with measured affinity, of 155 total; 47 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

CompoundAssayTypeValueUnit
2-fluoro-3-[6-[1-(4-fluoro-3-hydroxyphenyl)ethenyl]-2-pyridinyl]phenol1360338: Binding affinity to recombinant 17beta-HSD14 (unknown origin) expressed in Escherichia coli BL21 pLysS strain using E2 as substrate in presence of NAD measured continuously for 15 mins by fluorimetric methodki0.0015uM
(4-fluoro-3-hydroxyphenyl)-(6-methylquinolin-2-yl)methanone1360338: Binding affinity to recombinant 17beta-HSD14 (unknown origin) expressed in Escherichia coli BL21 pLysS strain using E2 as substrate in presence of NAD measured continuously for 15 mins by fluorimetric methodki0.0060uM
(4-fluoro-3-hydroxyphenyl)-naphthalen-2-ylmethanone1360338: Binding affinity to recombinant 17beta-HSD14 (unknown origin) expressed in Escherichia coli BL21 pLysS strain using E2 as substrate in presence of NAD measured continuously for 15 mins by fluorimetric methodki0.0060uM
(4-fluoro-3-hydroxyphenyl)-[7-(4-hydroxy-3-methylphenyl)quinolin-2-yl]methanone1360338: Binding affinity to recombinant 17beta-HSD14 (unknown origin) expressed in Escherichia coli BL21 pLysS strain using E2 as substrate in presence of NAD measured continuously for 15 mins by fluorimetric methodki0.0060uM
[6-[3-(dimethylamino)phenyl]-2-pyridinyl]-(4-fluoro-3-hydroxyphenyl)methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.0070uM
(4-fluoro-3-hydroxyphenyl)-[6-(3-hydroxyphenyl)-2-pyridinyl]methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.0070uM
[6-(3,4-dihydroxyphenyl)-2-pyridinyl]-(4-fluoro-3-hydroxyphenyl)methanone1313658: Inhibition of human 17-beta-HSD14 using estradiol as substrate after 15 mins by fluorimetric assayki0.0070uM
(2,4-difluoro-3-hydroxyphenyl)-[6-(2-fluoro-3-hydroxyphenyl)-2-pyridinyl]methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.0090uM
2-(4-fluoro-3-hydroxybenzoyl)quinoline-7-carbonitrile1360338: Binding affinity to recombinant 17beta-HSD14 (unknown origin) expressed in Escherichia coli BL21 pLysS strain using E2 as substrate in presence of NAD measured continuously for 15 mins by fluorimetric methodki0.0090uM
[5,6-bis(4-hydroxy-3-methylphenyl)-2-pyridinyl]-(4-fluoro-3-hydroxyphenyl)methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.0090uM
(4-fluoro-3-hydroxyphenyl)-[7-(2H-tetrazol-5-yl)quinolin-2-yl]methanone1360338: Binding affinity to recombinant 17beta-HSD14 (unknown origin) expressed in Escherichia coli BL21 pLysS strain using E2 as substrate in presence of NAD measured continuously for 15 mins by fluorimetric methodki0.0100uM
(4-fluoro-2,3-dihydroxyphenyl)-[6-(2-fluoro-3-hydroxyphenyl)-2-pyridinyl]methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.0110uM
(4-fluoro-3-hydroxyphenyl)-quinolin-2-ylmethanone1360338: Binding affinity to recombinant 17beta-HSD14 (unknown origin) expressed in Escherichia coli BL21 pLysS strain using E2 as substrate in presence of NAD measured continuously for 15 mins by fluorimetric methodki0.0120uM
(4-fluoro-3-hydroxyphenyl)-[6-(2-fluoro-3-hydroxyphenyl)-2-pyridinyl]methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.0130uM
(4-fluoro-3-hydroxyphenyl)-[5-(2-fluoro-3-hydroxyphenyl)-6-(4-hydroxy-3-methylphenyl)-2-pyridinyl]methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.0150uM
(4-fluoro-3-hydroxyphenyl)-[5-(3-fluoro-4-hydroxyphenyl)-2-pyridinyl]methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.0170uM
(2,3-dihydroxyphenyl)-[3-(2-fluoro-3-hydroxyphenyl)phenyl]methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.0210uM
2-[[2-(4-fluoro-3-hydroxybenzoyl)quinolin-7-yl]amino]acetic acid1360338: Binding affinity to recombinant 17beta-HSD14 (unknown origin) expressed in Escherichia coli BL21 pLysS strain using E2 as substrate in presence of NAD measured continuously for 15 mins by fluorimetric methodki0.0230uM
(4-fluoro-3-hydroxyphenyl)-[5-(4-hydroxy-3-methylphenyl)-2-pyridinyl]methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.0240uM
(4-fluoro-3-hydroxyphenyl)-[6-(4-hydroxy-3-methylphenyl)-2-pyridinyl]methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.0260uM
(7-aminoquinolin-2-yl)-(4-fluoro-3-hydroxyphenyl)methanone1360338: Binding affinity to recombinant 17beta-HSD14 (unknown origin) expressed in Escherichia coli BL21 pLysS strain using E2 as substrate in presence of NAD measured continuously for 15 mins by fluorimetric methodki0.0340uM
(4-fluoro-3-hydroxyphenyl)-[6-(3-fluoro-4-hydroxyphenyl)-2-pyridinyl]methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.0360uM
2-fluoro-3-[6-(4-fluoro-3-hydroxy-N-methylanilino)-2-pyridinyl]phenol1360338: Binding affinity to recombinant 17beta-HSD14 (unknown origin) expressed in Escherichia coli BL21 pLysS strain using E2 as substrate in presence of NAD measured continuously for 15 mins by fluorimetric methodki0.0420uM
(2,3-dihydroxyphenyl)-[6-(3-hydroxyphenyl)-2-pyridinyl]methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.0440uM
(4-fluoro-3-hydroxyphenyl)-[6-(3-hydroxy-4-methylphenyl)-2-pyridinyl]methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.0470uM
2-fluoro-3-[6-(4-fluoro-3-hydroxyanilino)-2-pyridinyl]phenol1360338: Binding affinity to recombinant 17beta-HSD14 (unknown origin) expressed in Escherichia coli BL21 pLysS strain using E2 as substrate in presence of NAD measured continuously for 15 mins by fluorimetric methodki0.0470uM
(4-fluoro-3-hydroxyphenyl)-(6-phenoxy-2-pyridinyl)methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.0500uM
2-fluoro-3-[6-(4-fluoro-3-hydroxyphenoxy)-2-pyridinyl]phenol1360338: Binding affinity to recombinant 17beta-HSD14 (unknown origin) expressed in Escherichia coli BL21 pLysS strain using E2 as substrate in presence of NAD measured continuously for 15 mins by fluorimetric methodki0.0580uM
(4-fluoro-3-hydroxyphenyl)-(6-phenyl-2-pyridinyl)methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.0630uM
(2,3-dihydroxyphenyl)-[6-(2-fluoro-3-hydroxyphenyl)-2-pyridinyl]methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.0640uM
(4-fluoro-3-hydroxyphenyl)-[6-(2-fluoro-3-hydroxyphenyl)-4-(hydroxymethyl)-2-pyridinyl]methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.0860uM
(4-fluoro-3-hydroxyphenyl)-(6-thiophen-3-yl-2-pyridinyl)methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.0970uM
(4-fluoro-3-hydroxyphenyl)-(6-hydroxyquinolin-2-yl)methanone1360338: Binding affinity to recombinant 17beta-HSD14 (unknown origin) expressed in Escherichia coli BL21 pLysS strain using E2 as substrate in presence of NAD measured continuously for 15 mins by fluorimetric methodki0.1190uM
3-[(Z)-[6-(2-fluoro-3-hydroxyphenyl)-1-hydroxy-2-pyridinylidene]-hydroxymethyl]cyclohexa-3,5-diene-1,2-dione1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.1320uM
[6-(2-fluoro-3-hydroxyphenyl)-2-pyridinyl]-(2-hydroxyphenyl)methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.1350uM
(4-fluoro-3-hydroxyphenyl)-[6-(4-methylpiperazin-1-yl)-2-pyridinyl]methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.1900uM
[6-(3-chloro-4-fluorophenyl)-2-pyridinyl]-(4-fluoro-3-hydroxyphenyl)methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.1900uM
(4-fluoro-3-hydroxyphenyl)-[6-(4-fluorophenyl)-2-pyridinyl]methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.2210uM
[6-(4-hydroxy-3-methylphenyl)-2-pyridinyl]-(3-hydroxyphenyl)methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.2450uM
6-(2-fluoro-3-hydroxyphenyl)-N-(4-fluoro-3-hydroxyphenyl)pyridine-2-carboxamide1360338: Binding affinity to recombinant 17beta-HSD14 (unknown origin) expressed in Escherichia coli BL21 pLysS strain using E2 as substrate in presence of NAD measured continuously for 15 mins by fluorimetric methodki0.3360uM
[6-(2-fluoro-3-hydroxyphenyl)-2-pyridinyl]-(2,3,4-trihydroxyphenyl)methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.4050uM
(4-fluoro-3-hydroxyphenyl)-(6-piperidin-1-yl-2-pyridinyl)methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.4070uM
[6-(3-fluoro-4-hydroxyphenyl)-2-pyridinyl]-(3-hydroxyphenyl)methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.4670uM
4-fluoro-N-[6-(2-fluoro-3-hydroxyphenyl)-2-pyridinyl]-3-hydroxybenzamide1360338: Binding affinity to recombinant 17beta-HSD14 (unknown origin) expressed in Escherichia coli BL21 pLysS strain using E2 as substrate in presence of NAD measured continuously for 15 mins by fluorimetric methodki0.6860uM
[6-(2-fluoro-3-hydroxyphenyl)-2-pyridinyl]-(2,3,6-trihydroxyphenyl)methanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki0.7960uM
(4-fluoro-3-hydroxyphenyl)-pyridin-2-ylmethanone1322642: Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assayki1.5410uM
4-fluoro-N-[6-(2-fluoro-3-hydroxyphenyl)-2-pyridinyl]-3-hydroxy-N-methylbenzamide1360338: Binding affinity to recombinant 17beta-HSD14 (unknown origin) expressed in Escherichia coli BL21 pLysS strain using E2 as substrate in presence of NAD measured continuously for 15 mins by fluorimetric methodki2.0300uM

CTD chemical–gene interactions

41 total (human), top 30 by PubMed support.

ChemicalActions (top 5)PubMed papers
bisphenol Adecreases expression, increases expression3
Benzo(a)pyreneaffects methylation2
Cisplatinaffects expression, affects cotreatment, increases expression2
Valproic Aciddecreases expression2
aristolochic acid Iincreases expression1
sotorasibdecreases expression, affects cotreatment1
trichostatin Adecreases expression1
tris(1,3-dichloro-2-propyl)phosphateincreases expression1
sodium arseniteincreases expression1
benzo(e)pyreneincreases methylation1
aflatoxin B2increases methylation1
avobenzoneincreases expression1
fipronilaffects cotreatment, increases expression1
abrineincreases expression1
jinfukangaffects cotreatment, increases expression1
trametinibaffects cotreatment, decreases expression1
NVP-BKM120affects cotreatment, decreases expression1
Temozolomidedecreases expression1
Decitabineaffects expression1
Sunitinibincreases expression1
Glyphosateincreases expression1
DEETaffects cotreatment, increases expression1
Estradioldecreases expression1
Fluoxetineincreases expression1
Hydrogen Peroxideaffects cotreatment, decreases expression1
Methapyrileneincreases methylation1
NADaffects binding, increases activity1
Niclosamideincreases expression1
Rotenoneincreases expression1
Silicon Dioxideincreases expression1

ChEMBL screening assays

13 unique, capped per target: 13 binding

Representative assays (with source publication via chembl_document):

Assay IDTypeDescriptionSource paper
CHEMBL3830811BindingInhibition of human 17-beta-HSD14 using estradiol as substrate after 15 mins by fluorimetric assayNew Insights into Human 17β-Hydroxysteroid Dehydrogenase Type 14: First Crystal Structures in Complex with a Steroidal Ligand and with a Potent Nonsteroidal Inhibitor. — J Med Chem

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.

No linked Atlas pages yet — the cross-entity mesh grows as the corpus expands.