KCNG4

Summary

KCNG4 (potassium voltage-gated channel modifier subfamily G member 4, HGNC:19697) is a protein-coding gene on chromosome 16q24.1, encoding Voltage-gated potassium channel regulatory subunit KCNG4 (Q8TDN1). Regulatory subunit of the voltage-gated potassium (Kv) channel which, when coassembled with KCNB1, modulates the kinetics parameters of the heterotetrameric channel namely the time course of activation, deactivation and inactivation and on the voltage-dependence of activation.

Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, subfamily G. This member functions as a modulatory subunit. The gene has strong expression in brain. Multiple alternatively spliced variants have been found in normal and cancerous tissues.

Source: NCBI Gene 93107 — RefSeq curated summary.

At a glance

• GWAS associations: 3
• Clinical variants (ClinVar): 134 total
• Druggable target: yes
• MANE Select transcript: NM_172347

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:19697
Approved symbol	KCNG4
Name	potassium voltage-gated channel modifier subfamily G member 4
Location	16q24.1
Locus type	gene with protein product
Status	Approved
Aliases	Kv6.4
Ensembl gene	ENSG00000168418
Ensembl biotype	protein_coding
OMIM	607603
Entrez	93107

Gene structure

Transcript identifiers

Ensembl transcripts: 2 — 2 protein_coding

ENST00000308251, ENST00000568181

RefSeq mRNA: 1 — MANE Select: NM_172347 NM_172347

CCDS: CCDS10945

Canonical transcript exons

ENST00000308251 — 3 exons

Exon	Start	End
ENSE00001190857	84236730	84237525
ENSE00001190862	84218657	84223020
ENSE00001860611	84239670	84240012

Expression profiles

Bgee: expression breadth broad, 40 present calls, max score 68.81.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 0.0170 / max 5.1472, expressed in 8 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
158343	0.0170	8

Top tissues by expression

244 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	68.81	silver quality
endothelial cell	CL:0000115	59.89	gold quality
epithelium of nasopharynx	UBERON:0001951	59.67	gold quality
secondary oocyte	CL:0000655	58.28	gold quality
sperm	CL:0000019	54.97	gold quality
skin of hip	UBERON:0001554	54.37	silver quality
amygdala	UBERON:0001876	50.97	gold quality
substantia nigra	UBERON:0002038	49.76	gold quality
midbrain	UBERON:0001891	48.92	gold quality
hypothalamus	UBERON:0001898	48.17	gold quality
lateral globus pallidus	UBERON:0002476	47.79	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	47.18	gold quality
oocyte	CL:0000023	46.59	gold quality
spinal cord	UBERON:0002240	46.51	gold quality
temporal lobe	UBERON:0001871	46.26	gold quality
buccal mucosa cell	CL:0002336	46.12	gold quality
prefrontal cortex	UBERON:0000451	46.04	gold quality
saphenous vein	UBERON:0007318	45.76	gold quality
Ammon’s horn	UBERON:0001954	45.73	gold quality
upper leg skin	UBERON:0004262	44.77	silver quality
putamen	UBERON:0001874	44.34	gold quality
bone marrow cell	CL:0002092	44.12	gold quality
amniotic fluid	UBERON:0000173	43.90	gold quality
anterior cingulate cortex	UBERON:0009835	43.56	gold quality
skeletal muscle tissue of rectus abdominis	UBERON:0004511	43.37	gold quality
nipple	UBERON:0002030	43.01	gold quality
trigeminal ganglion	UBERON:0001675	42.99	gold quality
sural nerve	UBERON:0015488	42.80	gold quality
trachea	UBERON:0003126	42.77	gold quality
neocortex	UBERON:0001950	42.42	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	3.59

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): E2F1

miRNA regulators (miRDB)

67 targeting KCNG4, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-4747-5P	100.00	67.90	2681
HSA-MIR-5196-5P	100.00	67.98	2761
HSA-MIR-4533	100.00	69.48	2758
HSA-MIR-4692	100.00	67.32	2066
HSA-MIR-574-5P	100.00	66.01	989
HSA-MIR-6758-5P	100.00	66.21	1470
HSA-MIR-6856-5P	100.00	65.47	1298
HSA-MIR-4514	99.99	67.10	1870
HSA-MIR-6825-5P	99.96	69.81	3431
HSA-MIR-6721-5P	99.93	68.92	2981
HSA-MIR-497-5P	99.92	71.83	2674
HSA-MIR-195-5P	99.90	72.81	2805
HSA-MIR-15A-5P	99.90	72.80	2787
HSA-MIR-15B-5P	99.90	72.78	2798
HSA-MIR-16-5P	99.90	72.80	2780
HSA-MIR-424-5P	99.89	71.90	2641
HSA-MIR-6838-5P	99.89	71.94	2690
HSA-MIR-7845-5P	99.88	64.88	771
HSA-MIR-6124	99.87	69.78	3551
HSA-MIR-3934-3P	99.76	65.51	1351
HSA-MIR-3150A-3P	99.76	64.44	1640
HSA-MIR-6763-5P	99.76	64.68	1767
HSA-MIR-6764-5P	99.75	67.89	2304
HSA-MIR-4530	99.69	66.47	1509
HSA-MIR-6766-5P	99.68	67.70	2325
HSA-MIR-7157-5P	99.66	69.33	1829
HSA-MIR-3175	99.65	66.30	2031
HSA-MIR-7150	99.62	66.80	1322
HSA-MIR-4310	99.59	68.84	2527
HSA-MIR-4524A-5P	99.57	71.73	1193

Literature-anchored findings (GeneRIF, showing 7)

• Obligatory heterotetramerization of three previously uncharacterized Kv channel subunits identified in human genome (Kv6.3)(Kv10.1) (Kv11.1) (PMID:12060745)
• This study suggests that the silent behaviour of Kv6.3 is largely caused by the C-terminal part of its sixth transmembrane domain that causes ER retention of the subunit. (PMID:16096342)
• Mutation of histidine 105 in the T1 domain of the potassium channel Kv2.1 disrupts heteromerization with Kv6.3 and Kv6.4. (PMID:19074135)
• KCNE5 subunits may affect Kv2.1 homotetramers and Kv2.1/Kv6.4 heterotetramers in vivo, resulting in more tissue-specific fine-tuning mechanisms. (PMID:26242757)
• KvS subunits modify the pharmacological response of Kv2 subunits when assembled in heterotetramers and illustrate the potential of KvS subunits to provide unique properties to the heterotetramers, as is the case for 4-AP on Kv2.1/Kv6.4 channels. (PMID:26505474)
• Human Labor Pain Is Influenced by the Voltage-Gated Potassium Channel KV6.4 Subunit. (PMID:32697988)
• KCNG4 Genetic Variant Linked to Migraine Prevents Expression of KCNB1. (PMID:39201645)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	kcng4b	ENSDARG00000051892
danio_rerio	kcng4a	ENSDARG00000062967
mus_musculus	Kcng4	ENSMUSG00000045246
rattus_norvegicus	Kcng4	ENSRNOG00000015746

Paralogs (31): KCNG1 (ENSG00000026559), KCNQ1 (ENSG00000053918), KCNQ2 (ENSG00000075043), KCND1 (ENSG00000102057), KCNA7 (ENSG00000104848), KCNA1 (ENSG00000111262), KCNC4 (ENSG00000116396), KCNQ4 (ENSG00000117013), KCNS1 (ENSG00000124134), KCNC1 (ENSG00000129159), KCNA5 (ENSG00000130037), KCNC3 (ENSG00000131398), KCNA10 (ENSG00000143105), KCNA6 (ENSG00000151079), KCNS2 (ENSG00000156486), KCNB1 (ENSG00000158445), KCNF1 (ENSG00000162975), KCNV1 (ENSG00000164794), KCNC2 (ENSG00000166006), KCNV2 (ENSG00000168263), KCNS3 (ENSG00000170745), KCNG3 (ENSG00000171126), KCND3 (ENSG00000171385), KCNA3 (ENSG00000177272), KCNA2 (ENSG00000177301), KCNG2 (ENSG00000178342), KCNA4 (ENSG00000182255), KCNB2 (ENSG00000182674), KCNQ3 (ENSG00000184156), KCND2 (ENSG00000184408), KCNQ5 (ENSG00000185760)

Protein

Protein identifiers

Voltage-gated potassium channel regulatory subunit KCNG4 — Q8TDN1 (reviewed: Q8TDN1)

Alternative names: Potassium voltage-gated channel subfamily G member 4, Voltage-gated potassium channel subunit Kv6.3, Voltage-gated potassium channel subunit Kv6.4

All UniProt accessions (2): Q8TDN1, Q547S7

UniProt curated annotations — full annotation on UniProt →

Function. Regulatory subunit of the voltage-gated potassium (Kv) channel which, when coassembled with KCNB1, modulates the kinetics parameters of the heterotetrameric channel namely the time course of activation, deactivation and inactivation and on the voltage-dependence of activation. Potassium channel subunit that does not form functional channels by itself. Reduces the deactivation rate. Modulates the threshold for activation by shifting by approximately 20 mV in hyperpolarizing direction. Markedly changes the inactivation by shifting the voltage dependence of inactivation by approximately 40 mV in hyperpolarizing direction. Acceleratee activation and enhances the time course of activation.

Subunit / interactions. Heterotetramer with KCNB1. Does not form homomultimer.

Subcellular location. Cell membrane.

Tissue specificity. Highly expressed in brain, and at lower levels in liver, small intestine and colon.

Domain organisation. The transmembrane segment S4 functions as a voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Channel opening and closing is effected by a conformation change that affects the position and orientation of the voltage-sensor paddle formed by S3 and S4 within the membrane. A transmembrane electric field that is positive inside would push the positively charged S4 segment outwards, thereby opening the pore, while a field that is negative inside would pull the S4 segment inwards and close the pore. Changes in the position and orientation of S4 are then transmitted to the activation gate formed by the inner helix bundle via the S4-S5 linker region.

Similarity. Belongs to the potassium channel family. G (TC 1.A.1.2) subfamily. Kv6.4/KCNG4 sub-subfamily.

Isoforms (2)

UniProt ID	Names	Canonical?
Q8TDN1-1	1	yes
Q8TDN1-2	2

RefSeq proteins (1): NP_758857* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR003131	T1-type_BTB	Domain
IPR003968	K_chnl_volt-dep_Kv	Family
IPR003971	K_chnl_volt-dep_Kv5/Kv9	Family
IPR005821	Ion_trans_dom	Domain
IPR011333	SKP1/BTB/POZ_sf	Homologous_superfamily
IPR027359	Volt_channel_dom_sf	Homologous_superfamily
IPR028325	VG_K_chnl	Family

Pfam: PF00520, PF02214

UniProt features (27 total): topological domain 8, transmembrane region 6, sequence variant 6, intramembrane region 2, splice variant 2, chain 1, region of interest 1, short sequence motif 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q8TDN1-F1	78.07	0.33

Function

Pathways and Gene Ontology

Reactome pathways

3 pathways

ID	Pathway
R-HSA-1296072	Voltage gated Potassium channels
R-HSA-112316	Neuronal System
R-HSA-1296071	Potassium Channels

MSigDB gene sets: 84 (showing top): GOBP_POTASSIUM_ION_TRANSPORT, REACTOME_VOLTAGE_GATED_POTASSIUM_CHANNELS, REACTOME_POTASSIUM_CHANNELS, TGACCTY_ERR1_Q2, GOBP_MONOATOMIC_CATION_TRANSPORT, GOBP_PROTEIN_HOMOOLIGOMERIZATION, GOBP_REGULATION_OF_TRANSPORT, GOBP_REGULATION_OF_MONOATOMIC_ION_TRANSPORT, GOBP_TRANSMEMBRANE_TRANSPORT, TGACCTTG_SF1_Q6, GOBP_REGULATION_OF_POTASSIUM_ION_TRANSPORT, AP4_01, GOBP_REGULATION_OF_TRANSMEMBRANE_TRANSPORT, LEIN_PONS_MARKERS, LEIN_MEDULLA_MARKERS

GO Biological Process (9): action potential (GO:0001508), regulation of potassium ion transport (GO:0043266), protein homooligomerization (GO:0051260), potassium ion transmembrane transport (GO:0071805), regulation of potassium ion transmembrane transport (GO:1901379), monoatomic ion transport (GO:0006811), potassium ion transport (GO:0006813), monoatomic ion transmembrane transport (GO:0034220), transmembrane transport (GO:0055085)

GO Molecular Function (5): voltage-gated potassium channel activity (GO:0005249), potassium channel regulator activity (GO:0015459), transmembrane transporter binding (GO:0044325), monoatomic ion channel activity (GO:0005216), potassium channel activity (GO:0005267)

GO Cellular Component (5): nucleoplasm (GO:0005654), plasma membrane (GO:0005886), voltage-gated potassium channel complex (GO:0008076), membrane (GO:0016020), monoatomic ion channel complex (GO:0034702)

Reactome top-level categories

Rollup of top-2 pathways:

Category	Pathways
Potassium Channels	1
Neuronal System	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
potassium ion transport	2
transport	2
potassium channel activity	2
cellular anatomical structure	2
regulation of membrane potential	1
regulation of metal ion transport	1
protein complex oligomerization	1
monoatomic cation transmembrane transport	1
regulation of potassium ion transport	1
potassium ion transmembrane transport	1
regulation of monoatomic cation transmembrane transport	1
metal ion transport	1
monoatomic ion transport	1
transmembrane transport	1
cellular process	1
voltage-gated monoatomic cation channel activity	1
ion channel regulator activity	1
protein binding	1
monoatomic ion transmembrane transporter activity	1
channel activity	1
monoatomic cation channel activity	1
potassium ion transmembrane transporter activity	1
nuclear lumen	1
membrane	1
cell periphery	1
potassium channel complex	1
plasma membrane protein complex	1
transmembrane transporter complex	1

Protein interactions and networks

STRING

1778 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
KCNG4	KCNB1	Q14721	860
KCNG4	CACNA1S	Q13698	487
KCNG4	KCNAB1	Q14722	485
KCNG4	KCNAB2	Q13303	462
KCNG4	KCNB2	Q92953	447
KCNG4	OPN4	Q9UHM6	443
KCNG4	NETO1	Q8TDF5	421
KCNG4	NXPH4	O95158	407
KCNG4	TMC8	Q8IU68	406
KCNG4	KCNH8	Q96L42	404
KCNG4	CNTN5	O94779	403
KCNG4	SDK2	Q58EX2	401
KCNG4	ZNF799	Q96GE5	395
KCNG4	LRRTM1	Q86UE6	388
KCNG4	KCNH7	Q9NS40	386

IntAct

3 interactions, top by confidence:

A	B	Type	Score
KCNG4	KCNB2	psi-mi:“MI:0915”(physical association)	0.400
KCNG4	CA1	psi-mi:“MI:0914”(association)	0.350

BioGRID (25): CST4 (Affinity Capture-MS), CST1 (Affinity Capture-MS), CST2 (Affinity Capture-MS), KCNB1 (FRET), KCNG4 (FRET), CST2 (Affinity Capture-MS), CST4 (Affinity Capture-MS), CST1 (Affinity Capture-MS), KCNG4 (Affinity Capture-MS), KCNB2 (Affinity Capture-MS), PPP1R15B (Affinity Capture-MS), AXL (Affinity Capture-MS), DCAKD (Affinity Capture-MS), ATF6 (Affinity Capture-MS), SREBF1 (Affinity Capture-MS)

ESM2 similar proteins: A2BDX4, A4K2T1, A4K2Y2, D4AD53, O15554, O73606, O88454, O89109, P15388, P17971, P17972, P35739, P48547, P59053, P59994, P59995, P97557, Q03719, Q0P583, Q17ST2, Q52PG9, Q5RC10, Q60565, Q63881, Q6IVV8, Q6PIU1, Q7TN37, Q80XM3, Q8BZN2, Q8CFS6, Q8HYZ1, Q8IV77, Q8R1P5, Q8R523, Q8TAE7, Q8TD43, Q8TDN1, Q8TDN2, Q96RP8, Q9ERS0

Diamond homologs: A2BDX4, A4K2M4, A4K2N8, A4K2P6, A4K2Q6, A4K2R3, A4K2S2, A4K2T1, A4K2V2, A4K2W6, A4K2X4, A4K2Y2, A6H8H5, D4AD53, D4ADX7, G5EFC3, O18868, O35173, O35174, O73606, O88758, O88759, P10499, P15384, P15385, P15387, P15388, P16388, P16390, P17970, P17971, P17972, P22001, P22459, P22739, P25122, P48547, P59053, P59994, P59995

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

134 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	123
Likely benign	5
Benign	5

Top pathogenic / likely-pathogenic (0)

SpliceAI

518 predictions. Top by Δscore:

Variant	Effect	Δscore
16:84237534:C:T	acceptor_gain	1.0000
16:84237534:C:CT	acceptor_gain	0.9900
16:84237535:A:T	acceptor_gain	0.9900
16:84239665:CTCA:C	donor_loss	0.9900
16:84239667:CA:C	donor_loss	0.9900
16:84239668:ACCT:A	donor_loss	0.9900
16:84239669:C:G	donor_loss	0.9900
16:84239669:CCTT:C	donor_gain	0.9900
16:84239668:A:AC	donor_gain	0.9800
16:84239669:C:CC	donor_gain	0.9800
16:84239668:ACCTT:A	donor_gain	0.9600
16:84239669:CCTTC:C	donor_gain	0.9600
16:84239668:AC:A	donor_gain	0.9500
16:84239669:CC:C	donor_gain	0.9500
16:84237533:C:CT	acceptor_gain	0.9400
16:84239669:CCT:C	donor_gain	0.9400
16:84237521:CCAGT:C	acceptor_gain	0.9100
16:84237522:CAGTC:C	acceptor_gain	0.9100
16:84236724:GCTCA:G	donor_loss	0.8900
16:84236725:CTCAC:C	donor_loss	0.8900
16:84236726:TCACC:T	donor_loss	0.8900
16:84236727:CACCT:C	donor_loss	0.8900
16:84236729:C:T	donor_loss	0.8900
16:84236722:CCGCT:C	donor_loss	0.8800
16:84236723:CGCTC:C	donor_loss	0.8800
16:84222216:G:C	donor_gain	0.8700
16:84222221:A:C	donor_gain	0.8700
16:84239145:T:TA	donor_gain	0.8700
16:84229747:CAGTG:C	donor_gain	0.8600
16:84236730:C:G	donor_loss	0.8500

AlphaMissense

3393 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
16:84222454:G:C	S441R	0.998
16:84222454:G:T	S441R	0.998
16:84222456:T:G	S441R	0.998
16:84222463:G:C	S438R	0.998
16:84222463:G:T	S438R	0.998
16:84222465:T:G	S438R	0.998
16:84222546:A:G	W411R	0.998
16:84222546:A:T	W411R	0.998
16:84222565:G:C	S404R	0.998
16:84222565:G:T	S404R	0.998
16:84222567:T:G	S404R	0.998
16:84222958:G:C	F273L	0.998
16:84222958:G:T	F273L	0.998
16:84222960:A:G	F273L	0.998
16:84222406:G:C	F457L	0.997
16:84222406:G:T	F457L	0.997
16:84222408:A:G	F457L	0.997
16:84222466:G:C	S437R	0.997
16:84222466:G:T	S437R	0.997
16:84222468:T:G	S437R	0.997
16:84222500:G:T	P426H	0.997
16:84222571:G:C	F402L	0.997
16:84222571:G:T	F402L	0.997
16:84222573:A:G	F402L	0.997
16:84222881:T:A	D299V	0.997
16:84222963:A:G	W272R	0.997
16:84222963:A:T	W272R	0.997
16:84222452:C:T	G442E	0.996
16:84222485:C:T	G431D	0.996
16:84222544:C:A	W411C	0.996

dbSNP variants (sampled 300 via entrez): RS1000012795 (16:84238881 C>A), RS1000016728 (16:84226772 G>A,C), RS1000106886 (16:84228495 C>G,T), RS1000118187 (16:84228591 C>A,T), RS1000206938 (16:84240863 T>A), RS1000263508 (16:84218339 C>A,G,T), RS1000293169 (16:84218173 G>A,T), RS1000308309 (16:84236869 C>A,T), RS1000382902 (16:84236709 A>G), RS1000400929 (16:84241897 C>G,T), RS1000464475 (16:84220941 A>G), RS1000540181 (16:84238211 G>A,C), RS1000600242 (16:84240682 A>G), RS1000725331 (16:84221207 C>G), RS1000907255 (16:84225649 T>C)

Disease associations

OMIM: gene MIM:607603 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

3 associations (top):

Study	Trait	p-value
GCST001523_23	Visceral adipose tissue adjusted for BMI	6.000000e-06
GCST003830_11	Response to bronchodilator in chronic obstructive pulmonary disease (change in FEV1)	7.000000e-07
GCST011891_4	Systolic blood pressure (baseline)	2.000000e-08

EFO canonical traits (3, from GWAS)

EFO ID	Trait name
EFO:0004340	body mass index
EFO:0005921	FEV change measurement
EFO:0006335	systolic blood pressure

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL2362996 (PROTEIN FAMILY)

PharmGKB: 1 entry (VIP=true, CPIC=false)

GtoPdb / IUPHAR curated pharmacology

(IUPHAR/BPS Guide to Pharmacology — expert-curated)

Target class: vgic — Voltage-gated potassium channels (K_v)

ChEMBL bioactivities

2 potent at pChembl≥5 of 2 total, top 2 by pChembl (potency: 10 = 0.1 nM, 6 = 1 µM).

pChembl	Type	Value	Unit	Molecule
9.82	Ki	0.15	nM	CHEMBL5722941
9.74	IC50	0.18	nM	CHEMBL5722941

PubChem BioAssay actives

2 with measured affinity, of 34 total; 1 most potent distinct compounds. Largely complementary to BindingDB; screening values are coarse (µM, 4 dp), so sub-nM hits tie at the floor.

Compound	Assay	Type	Value	Unit
3-[(1R,2aS,4S,5aS,8aS,10S,11aR,14aS,16S,17aS,19S,20aS,22S,23aS,25S,26aS,28S,29aS,31R,32aS,35aS,36R,38aS,39S,41aS,42S,44aS,45S,48R,50aS,51S,53aS,54S,56aS,57S,59aS,60S,63S,66S,69S,72S,75S,78S,87R,93S,96S,99S)-17a,20a,23a,53a,63-pentakis(4-aminobutyl)-31-[[(2S)-2-[[(2S)-6-amino-2-[[(2S)-5-carbamimidamido-2-[[(2S)-5-carbamimidamido-2-[[(2S)-1-[(2S)-5-oxopyrrolidine-2-carbonyl]pyrrolidine-2-carbonyl]amino]pentanoyl]amino]pentanoyl]amino]hexanoyl]amino]-4-methylpentanoyl]amino]-16,29a,72,78-tetrakis(2-amino-2-oxoethyl)-14a,26a-bis(3-amino-3-oxopropyl)-2a,38a,66-tribenzyl-28,50a,57-tris[(2S)-butan-2-yl]-4,5a,19,42,45,69-hexakis(3-carbamimidamidopropyl)-51,54-bis(2-carboxyethyl)-56a,99-bis(carboxymethyl)-36-[[(2S,3S)-1-(carboxymethylamino)-3-methyl-1-oxopentan-2-yl]carbamoyl]-39,60-bis[(1R)-1-hydroxyethyl]-75,93-bis(hydroxymethyl)-32a,35a,59a-tris[(4-hydroxyphenyl)methyl]-22-(1H-imidazol-4-ylmethyl)-96-(1H-indol-3-ylmethyl)-41a-methyl-25-(2-methylpropyl)-1a,3,4a,6,7a,9,10a,13a,15,16a,18,19a,21,22a,24,25a,27,28a,30,31a,34a,37a,38,40a,41,43a,44,47,49a,50,52a,53,55a,56,58a,59,61a,62,65,68,71,74,77,80,83,86,89,92,95,98-pentacontaoxo-33,34,63a,64a,67a,68a-hexathia-a,2,3a,5,6a,8,9a,12a,14,15a,17,18a,20,21a,23,24a,26,27a,29,30a,33a,36a,37,39a,40,42a,43,46,48a,49,51a,52,54a,55,57a,58,60a,61,64,67,70,73,76,79,82,85,88,91,94,97-pentacontazapentacyclo[85.74.4.448,111.010,14.0144,148]nonahexacontahectan-8a-yl]propanoic acid	2198828: Binding affinity to KV channel (unknown origin) assessed as inhibition constant	ki	0.0001	uM

CTD chemical–gene interactions

10 total (human), top 10 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Resveratrol	affects cotreatment, decreases expression	2
bisphenol A	decreases methylation	1
S-(1,2-dichlorovinyl)cysteine	increases expression, affects response to substance	1
bisphenol S	decreases methylation	1
Acetaminophen	increases expression	1
Benzo(a)pyrene	affects methylation, increases methylation	1
Copper	affects cotreatment, decreases expression	1
Lipopolysaccharides	decreases expression, affects response to substance, increases expression	1
Plant Extracts	affects cotreatment, decreases expression	1
Valproic Acid	increases methylation	1

ChEMBL screening assays

21 unique, capped per target: 20 binding, 1 toxicity

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL1787442	Binding	Inhibition of human recombinant Kv channel at 10 uM by radioligand binding assay	Structure-activity relationships of pyrrole based S-nitrosoglutathione reductase inhibitors: pyrrole regioisomers and propionic acid replacement. — Bioorg Med Chem Lett
CHEMBL5522525	Toxicity	Inhibition of human K+ channel by automated electrophysiology	Discovery of Clinical Candidate AZD5462, a Selective Oral Allosteric RXFP1 Agonist for Treatment of Heart Failure. — J Med Chem

Cellosaurus cell lines

1 cell lines: 1 transformed cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_YA61	IDG-HEK293T-KCNG4-V5-OE	Transformed cell line	Female

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.