KIF25

Summary

KIF25 (kinesin family member 25, HGNC:6390) is a protein-coding gene on chromosome 6q27, encoding Kinesin-like protein KIF25 (Q9UIL4). Minus-end microtubule-dependent motor protein.

The protein encoded by this gene is a member of the kinesin-like protein family. Protein family members are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. However, the particular function of this gene product has not yet been determined. Two alternatively spliced transcript variants which encode products have been described. Other splice variants have been found that lack exon 2 and the initiation codon for translation.

Source: NCBI Gene 3834 — RefSeq curated summary.

At a glance

• GWAS associations: 5
• Clinical variants (ClinVar): 84 total
• MANE Select transcript: NM_030615

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:6390
Approved symbol	KIF25
Name	kinesin family member 25
Location	6q27
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000125337
Ensembl biotype	protein_coding
OMIM	603815
Entrez	3834

Gene structure

Transcript identifiers

Ensembl transcripts: 9 — 4 protein_coding, 4 retained_intron, 1 protein_coding_CDS_not_defined

ENST00000443060, ENST00000496008, ENST00000504593, ENST00000515361, ENST00000643607, ENST00000644536, ENST00000645382, ENST00000652547, ENST00000955753

RefSeq mRNA: 2 — MANE Select: NM_030615 NM_005355, NM_030615

CCDS: CCDS5305

Canonical transcript exons

ENST00000643607 — 13 exons

Exon	Start	End
ENSE00001150227	168042561	168042716
ENSE00001150232	168041969	168042151
ENSE00001150241	168040065	168040216
ENSE00001150248	168038553	168038729
ENSE00001150259	168030773	168030847
ENSE00001164042	168029492	168029677
ENSE00002029735	168003614	168003703
ENSE00002061661	168017973	168018040
ENSE00002068602	167999092	167999320
ENSE00002083639	168002543	168002659
ENSE00003499889	168033882	168034031
ENSE00003815819	167997671	167998870
ENSE00003820921	168044827	168045091

Expression profiles

Bgee: expression breadth ubiquitous, 151 present calls, max score 87.05.

Top tissues by expression

249 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	87.05	gold quality
buccal mucosa cell	CL:0002336	77.45	silver quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	76.91	silver quality
myocardium	UBERON:0002349	73.68	gold quality
right hemisphere of cerebellum	UBERON:0014890	72.01	gold quality
cerebellar hemisphere	UBERON:0002245	71.36	gold quality
cerebellar cortex	UBERON:0002129	71.26	gold quality
apex of heart	UBERON:0002098	70.70	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	70.70	gold quality
cerebellum	UBERON:0002037	70.48	gold quality
adipose tissue of abdominal region	UBERON:0007808	70.22	gold quality
omental fat pad	UBERON:0010414	70.03	gold quality
peritoneum	UBERON:0002358	70.01	gold quality
right frontal lobe	UBERON:0002810	69.01	gold quality
spinal cord	UBERON:0002240	68.08	gold quality
putamen	UBERON:0001874	67.54	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	67.11	gold quality
Brodmann (1909) area 9	UBERON:0013540	67.03	gold quality
parotid gland	UBERON:0001831	66.94	gold quality
adipose tissue	UBERON:0001013	66.44	gold quality
caudate nucleus	UBERON:0001873	66.10	gold quality
substantia nigra	UBERON:0002038	65.05	gold quality
nucleus accumbens	UBERON:0001882	65.02	gold quality
anterior cingulate cortex	UBERON:0009835	65.01	gold quality
skeletal muscle tissue of rectus abdominis	UBERON:0004511	64.67	gold quality
prefrontal cortex	UBERON:0000451	64.65	gold quality
amygdala	UBERON:0001876	64.63	gold quality
periodontal ligament	UBERON:0008266	64.39	gold quality
hypothalamus	UBERON:0001898	64.32	gold quality
frontal cortex	UBERON:0001870	63.95	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	2.78

Regulation

Is transcription factor: no

Literature-anchored findings (GeneRIF, showing 1)

• The activity of Kif25 during interphase is required to maintain a centred nucleus to ensure the spindle is stably oriented at the onset of mitosis. (PMID:28263957)

Cross-species orthologs

1 orthologs

Organism	Symbol	Gene ID
danio_rerio	kif25	ENSDARG00000017532

Paralogs (41): KIF1B (ENSG00000054523), KIF26A (ENSG00000066735), KIF2A (ENSG00000068796), KIF22 (ENSG00000079616), KIF3C (ENSG00000084731), KIF9 (ENSG00000088727), KIF16B (ENSG00000089177), KIF4A (ENSG00000090889), KIF3B (ENSG00000101350), KIF20A (ENSG00000112984), KIF21B (ENSG00000116852), KIF17 (ENSG00000117245), KIF14 (ENSG00000118193), KIF18A (ENSG00000121621), KIF1C (ENSG00000129250), KIF1A (ENSG00000130294), KIF3A (ENSG00000131437), KIF12 (ENSG00000136883), KIF13A (ENSG00000137177), KIF23 (ENSG00000137807), KIF11 (ENSG00000138160), CENPE (ENSG00000138778), KIF21A (ENSG00000139116), KIFC3 (ENSG00000140859), KIF2B (ENSG00000141200), KIF2C (ENSG00000142945), KIF5A (ENSG00000155980), KIF26B (ENSG00000162849), KIF15 (ENSG00000163808), KIF6 (ENSG00000164627), KIF27 (ENSG00000165115), KIF7 (ENSG00000166813), KIFC2 (ENSG00000167702), KIF5C (ENSG00000168280), KIF5B (ENSG00000170759), KIF18B (ENSG00000186185), KIF24 (ENSG00000186638), KIF19 (ENSG00000196169), KIF13B (ENSG00000197892), KIF4B (ENSG00000226650)

Protein

Protein identifiers

Kinesin-like protein KIF25 — Q9UIL4 (reviewed: Q9UIL4)

Alternative names: Kinesin-like protein 3

All UniProt accessions (2): Q9UIL4, A0A494C0P5

UniProt curated annotations — full annotation on UniProt →

Function. Minus-end microtubule-dependent motor protein. Acts as a negative regulator of centrosome separation required to prevent premature centrosome separation during interphase. Required to maintain a centered nucleus to ensure that the spindle is stably oriented at the onset of mitosis. May also act as a negative regulator of amino acid starvation-induced autophagy.

Subunit / interactions. Homotetramer.

Subcellular location. Cytoplasm. Cytoskeleton. Microtubule organizing center. Centrosome.

Similarity. Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q9UIL4-1	1	yes
Q9UIL4-2	2

RefSeq proteins (2): NP_005346, NP_085118* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001752	Kinesin_motor_dom	Domain
IPR019821	Kinesin_motor_CS	Conserved_site
IPR027417	P-loop_NTPase	Homologous_superfamily
IPR027640	Kinesin-like_fam	Family
IPR036961	Kinesin_motor_dom_sf	Homologous_superfamily

Pfam: PF00225

UniProt features (12 total): sequence variant 4, region of interest 3, chain 1, domain 1, sequence conflict 1, binding site 1, splice variant 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9UIL4-F1	71.90	0.30

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (1): 65–72

Function

Pathways and Gene Ontology

Reactome pathways

8 pathways

ID	Pathway
R-HSA-6811434	COPI-dependent Golgi-to-ER retrograde traffic
R-HSA-983189	Kinesins
R-HSA-109582	Hemostasis
R-HSA-199991	Membrane Trafficking
R-HSA-5653656	Vesicle-mediated transport
R-HSA-6811442	Intra-Golgi and retrograde Golgi-to-ER traffic
R-HSA-8856688	Golgi-to-ER retrograde transport
R-HSA-983231	Factors involved in megakaryocyte development and platelet production

MSigDB gene sets: 101 (showing top): GOBP_CHROMOSOME_ORGANIZATION, GOBP_ESTABLISHMENT_OF_SPINDLE_ORIENTATION, GOBP_REGULATION_OF_AUTOPHAGY, GOBP_ESTABLISHMENT_OR_MAINTENANCE_OF_CELL_POLARITY, GOBP_PROTEIN_HOMOTETRAMERIZATION, GOBP_SPINDLE_LOCALIZATION, GOCC_KINESIN_COMPLEX, REACTOME_MEMBRANE_TRAFFICKING, GOCC_MICROTUBULE_ORGANIZING_CENTER, GOBP_NEGATIVE_REGULATION_OF_AUTOPHAGY, GOBP_ESTABLISHMENT_OF_CELL_POLARITY, GOBP_ORGANELLE_FISSION, GOBP_NEGATIVE_REGULATION_OF_CELL_CYCLE_PROCESS, GOBP_REGULATION_OF_CATABOLIC_PROCESS, GOBP_NEGATIVE_REGULATION_OF_CELL_CYCLE

GO Biological Process (8): mitotic sister chromatid segregation (GO:0000070), organelle organization (GO:0006996), microtubule-based movement (GO:0007018), negative regulation of autophagy (GO:0010507), negative regulation of mitotic centrosome separation (GO:0046603), protein homotetramerization (GO:0051289), establishment of spindle orientation (GO:0051294), nucleus localization (GO:0051647)

GO Molecular Function (7): microtubule motor activity (GO:0003777), ATP binding (GO:0005524), microtubule binding (GO:0008017), minus-end-directed microtubule motor activity (GO:0008569), ATP hydrolysis activity (GO:0016887), nucleotide binding (GO:0000166), cytoskeletal motor activity (GO:0003774)

GO Cellular Component (5): cytoplasm (GO:0005737), centrosome (GO:0005813), kinesin complex (GO:0005871), microtubule (GO:0005874), cytoskeleton (GO:0005856)

Reactome top-level categories

Rollup of top-6 pathways:

Category	Pathways
Golgi-to-ER retrograde transport	1
Factors involved in megakaryocyte development and platelet production	1
Vesicle-mediated transport	1
Membrane Trafficking	1
Intra-Golgi and retrograde Golgi-to-ER traffic	1
Hemostasis	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
ATP-dependent activity	2
sister chromatid segregation	1
mitotic nuclear division	1
mitotic cell cycle process	1
cellular component organization	1
microtubule-based process	1
autophagy	1
negative regulation of catabolic process	1
regulation of autophagy	1
mitotic centrosome separation	1
negative regulation of cell cycle process	1
regulation of mitotic centrosome separation	1
protein homooligomerization	1
protein tetramerization	1
establishment of cell polarity	1
establishment of spindle localization	1
organelle localization	1
cytoskeletal motor activity	1
polypeptide conformation or assembly isomerase activity	1
adenyl ribonucleotide binding	1
purine ribonucleoside triphosphate binding	1
tubulin binding	1
microtubule motor activity	1
ribonucleoside triphosphate phosphatase activity	1
nucleoside phosphate binding	1
heterocyclic compound binding	1
molecular_function	1
intracellular anatomical structure	1
cellular anatomical structure	1
centriole	1
microtubule organizing center	1
microtubule associated complex	1
microtubule cytoskeleton	1
polymeric cytoskeletal fiber	1
intracellular membraneless organelle	1

Protein interactions and networks

STRING

1699 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
KIF25	FRMD1	Q8N878	627
KIF25	DYNLT2	Q8IZS6	558
KIF25	DACT2	Q5SW24	557
KIF25	CEP170	Q5SW79	500
KIF25	ADAMTS18	Q8TE60	491
KIF25	PHF10	Q8WUB8	484
KIF25	WDR62	O43379	462
KIF25	SMOC2	Q9H3U7	459
KIF25	WDR27	A2RRH5	457
KIF25	CHRM5	P08912	437
KIF25	KRTAP19-7	Q3SYF9	433
KIF25	SFT2D1	Q8WV19	418
KIF25	PRG4	Q92954	411
KIF25	C1orf232	A0A0U1RR37	411
KIF25	LRG1	P02750	400
KIF25	A0A1W2PQ80	A0A1W2PQ80	400

IntAct

3 interactions, top by confidence:

A	B	Type	Score
KIF25	DDX19B	psi-mi:“MI:0915”(physical association)	0.400
MYC	PDZD2	psi-mi:“MI:0914”(association)	0.350

BioGRID (1): DDX19B (Affinity Capture-MS)

ESM2 similar proteins: A8WFU8, B7ZC32, B7ZNG0, D3YXS5, F4JGP4, F8WLE0, O35071, O35231, O35787, O43896, O95235, P18105, P38935, P40694, P46864, P46875, P79955, P97329, Q00653, Q07970, Q0J9V3, Q14147, Q15027, Q1HG60, Q29RT6, Q2M1P5, Q2R2P7, Q4R918, Q58G59, Q5I0E8, Q5XI63, Q5ZLK6, Q60443, Q60560, Q6P3R1, Q7ZYL5, Q80SX8, Q80TF6, Q8K2H4, Q96FN5

Diamond homologs: B7ZNG0, E9Q5G3, O16844, O95239, P27895, Q02241, Q292S8, Q2M1P5, Q4R918, Q6FXI5, Q91784, Q9UIL4, F4IIS5, F4JX00, O23826, Q58G59, A2ZRG4, A3BFT0, A8BB91, B2GU58, B3H6Z8, B7EJ91, B9EUM5, B9F7C8, B9FAF3, B9FL70, B9FTR1, B9G2X9, B9G8P1, F4HZF0, F4I1T9, F4IAR2, F4IBQ9, F4IJK6, F4IL57, F4J2M6, F4JGP4, F4K0J3, F4K4C5, G5EGS3

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

84 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	69
Likely benign	6
Benign	3

Top pathogenic / likely-pathogenic (0)

SpliceAI

1997 predictions. Top by Δscore:

Variant	Effect	Δscore
6:168017967:TCTCA:T	acceptor_loss	1.0000
6:168017968:CTCA:C	acceptor_loss	1.0000
6:168017969:TCAGG:T	acceptor_loss	1.0000
6:168017970:CAGGA:C	acceptor_loss	1.0000
6:168017971:A:AG	acceptor_gain	1.0000
6:168017971:AG:A	acceptor_gain	1.0000
6:168017972:G:GG	acceptor_gain	1.0000
6:168017972:GG:G	acceptor_gain	1.0000
6:168017972:GGAA:G	acceptor_gain	1.0000
6:168018036:GAAAG:G	donor_gain	1.0000
6:168018038:AAGGT:A	donor_loss	1.0000
6:168018039:AGGTA:A	donor_loss	1.0000
6:168018040:GGTA:G	donor_loss	1.0000
6:168018041:G:GG	donor_gain	1.0000
6:168018042:T:G	donor_loss	1.0000
6:168038548:CGCA:C	acceptor_loss	1.0000
6:168038549:GCAG:G	acceptor_loss	1.0000
6:168038550:CAGGC:C	acceptor_loss	1.0000
6:168038551:A:AG	acceptor_gain	1.0000
6:168038551:AG:A	acceptor_gain	1.0000
6:168038552:G:GT	acceptor_gain	1.0000
6:168038552:GG:G	acceptor_gain	1.0000
6:168038552:GGC:G	acceptor_gain	1.0000
6:168038552:GGCT:G	acceptor_gain	1.0000
6:168038552:GGCTC:G	acceptor_gain	1.0000
6:168038728:GA:G	donor_gain	1.0000
6:168038729:AGT:A	donor_loss	1.0000
6:168038730:G:GG	donor_gain	1.0000
6:168038730:GTG:G	donor_loss	1.0000
6:168038731:TGA:T	donor_loss	1.0000

AlphaMissense

0 scored. Top likely-pathogenic:

dbSNP variants (sampled 300 via entrez): RS1000026375 (6:168040419 A>G), RS1000075927 (6:168045228 G>A,C,T), RS1000160743 (6:168035252 C>G,T), RS1000210407 (6:167997966 T>C), RS1000233341 (6:168030192 G>A), RS1000303294 (6:167996199 C>A,G), RS1000359225 (6:168035706 T>C), RS1000517887 (6:168041292 C>A,G,T), RS1000530541 (6:168036819 T>G), RS1000603281 (6:167997718 A>G), RS1000743495 (6:168029326 A>G), RS1000778430 (6:168014607 CA>C), RS1000897415 (6:168020168 C>T), RS1000905711 (6:168020418 G>A), RS1000949782 (6:167998968 C>T)

Disease associations

OMIM: gene MIM:603815 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

5 associations (top):

Study	Trait	p-value
GCST001820_2	Metabolite levels (5-HIAA)	6.000000e-06
GCST006979_720	Heel bone mineral density	1.000000e-34
GCST012146_1	Hemoglobin levels	5.000000e-06
GCST012146_15	Hemoglobin levels	5.000000e-07
GCST90014243_2	Kawasaki disease	4.000000e-08

EFO canonical traits (3, from GWAS)

EFO ID	Trait name
EFO:0005132	5-HIAA measurement
EFO:0009270	heel bone mineral density
EFO:0004509	hemoglobin measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

18 total (human), top 18 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Benzo(a)pyrene	affects methylation	2
ethyl-p-hydroxybenzoate	decreases expression	1
mono-(2-ethylhexyl)phthalate	increases abundance, increases methylation	1
benzo(e)pyrene	increases methylation	1
aflatoxin B2	increases methylation	1
S-(1,2-dichlorovinyl)cysteine	decreases expression, affects response to substance, increases expression, affects cotreatment	1
bisphenol S	increases methylation, affects cotreatment	1
Fulvestrant	affects cotreatment, increases methylation	1
Arsenic	increases methylation	1
Diethylhexyl Phthalate	increases abundance, increases methylation	1
Lipopolysaccharides	decreases expression, affects response to substance, increases expression, affects cotreatment	1
Methapyrilene	increases methylation	1
Phthalic Acids	decreases methylation	1
Silicon Dioxide	decreases expression	1
Smoke	decreases expression	1
Tobacco Smoke Pollution	increases expression	1
Valproic Acid	decreases methylation	1
Aflatoxin B1	decreases methylation	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.