KIF4B

Summary

KIF4B (kinesin family member 4B, HGNC:6322) is a protein-coding gene on chromosome 5q33.2, encoding Chromosome-associated kinesin KIF4B (Q2VIQ3). Iron-sulfur (Fe-S) cluster binding motor protein that has a role in chromosome segregation during mitosis.

This gene is an intronless retrocopy of kinesin family member 4A. The protein encoded by this gene is a microtubule-based motor protein that plays vital roles in anaphase spindle dynamics and cytokinesis.

Source: NCBI Gene 285643 — RefSeq curated summary.

At a glance

• GWAS associations: 4
• Clinical variants (ClinVar): 194 total
• MANE Select transcript: NM_001099293

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:6322
Approved symbol	KIF4B
Name	kinesin family member 4B
Location	5q33.2
Locus type	gene with protein product
Status	Approved
Ensembl gene	ENSG00000226650
Ensembl biotype	protein_coding
OMIM	609184
Entrez	285643

Gene structure

Transcript identifiers

Ensembl transcripts: 1 — 1 protein_coding

ENST00000435029

RefSeq mRNA: 1 — MANE Select: NM_001099293 NM_001099293

CCDS: CCDS47324

Canonical transcript exons

ENST00000435029 — 1 exons

Exon	Start	End
ENSE00001805089	155013755	155018141

Expression profiles

Bgee: expression breadth tissue_specific, 6 present calls, max score 72.17.

Top tissues by expression

128 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	72.17	gold quality
testis	UBERON:0000473	56.39	gold quality
left testis	UBERON:0004533	55.53	gold quality
ventricular zone	UBERON:0003053	54.37	gold quality
right testis	UBERON:0004534	53.89	gold quality
sural nerve	UBERON:0015488	47.15	gold quality
skeletal muscle tissue	UBERON:0001134	44.22	gold quality
ganglionic eminence	UBERON:0004023	43.86	gold quality
muscle tissue	UBERON:0002385	42.30	gold quality
colonic epithelium	UBERON:0000397	41.34	gold quality
superior frontal gyrus	UBERON:0002661	40.53	gold quality
cortical plate	UBERON:0005343	39.91	gold quality
bone marrow cell	CL:0002092	39.80	gold quality
stromal cell of endometrium	CL:0002255	39.01	gold quality
bone marrow	UBERON:0002371	38.91	gold quality
hindlimb stylopod muscle	UBERON:0004252	38.70	gold quality
tonsil	UBERON:0002372	37.77	gold quality
placenta	UBERON:0001987	37.74	gold quality
smooth muscle tissue	UBERON:0001135	37.71	gold quality
vermiform appendix	UBERON:0001154	37.67	gold quality
prefrontal cortex	UBERON:0000451	36.64	gold quality
granulocyte	CL:0000094	34.88	gold quality
lymph node	UBERON:0000029	33.27	gold quality
cerebellar cortex	UBERON:0002129	33.04	gold quality
cerebellum	UBERON:0002037	33.00	gold quality
cerebellar hemisphere	UBERON:0002245	32.92	gold quality
leukocyte	CL:0000738	32.73	gold quality
monocyte	CL:0000576	32.69	gold quality
frontal cortex	UBERON:0001870	32.57	gold quality
duodenum	UBERON:0002114	32.41	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 0.

Experiment	Marker?	Max mean expression
E-ANND-3	no	2.42

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

33 targeting KIF4B, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-6873-3P	100.00	71.42	2626
HSA-MIR-150-5P	99.99	66.69	1976
HSA-MIR-5696	99.98	72.36	4487
HSA-MIR-4760-3P	99.93	70.50	2385
HSA-MIR-3143	99.93	71.96	3104
HSA-MIR-6515-3P	99.82	68.19	1933
HSA-MIR-4719	99.73	72.10	3329
HSA-MIR-4699-3P	99.71	70.15	3142
HSA-MIR-6751-5P	99.56	64.99	1145
HSA-MIR-510-3P	99.54	70.06	2965
HSA-MIR-7159-5P	99.53	72.12	2472
HSA-MIR-1275	99.47	67.90	2749
HSA-MIR-3064-5P	99.26	66.13	1497
HSA-MIR-3085-3P	99.26	66.16	1490
HSA-MIR-6504-5P	99.26	65.95	1487
HSA-MIR-4291	99.20	68.88	2969
HSA-MIR-6803-5P	99.19	63.90	1026
HSA-MIR-7109-5P	99.18	66.13	1057
HSA-MIR-3619-5P	99.00	68.87	2308
HSA-MIR-4451	98.82	68.17	1455
HSA-MIR-942-3P	98.81	69.04	876
HSA-MIR-767-3P	98.61	67.69	1192
HSA-MIR-1178-3P	98.57	67.09	890
HSA-MIR-1248	98.47	67.54	1314
HSA-MIR-6792-5P	98.39	68.16	1330
HSA-MIR-4665-5P	97.91	67.69	1536
HSA-MIR-4724-3P	97.57	67.31	785
HSA-MIR-937-5P	97.43	68.39	667
HSA-MIR-2278	97.30	66.19	1130
HSA-MIR-6729-3P	96.91	66.79	703

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Kif4-ps	ENSMUSG00000092054
rattus_norvegicus	Kif4b	ENSRNOG00000064692

Paralogs (41): KIF1B (ENSG00000054523), KIF26A (ENSG00000066735), KIF2A (ENSG00000068796), KIF22 (ENSG00000079616), KIF3C (ENSG00000084731), KIF9 (ENSG00000088727), KIF16B (ENSG00000089177), KIF4A (ENSG00000090889), KIF3B (ENSG00000101350), KIF20A (ENSG00000112984), KIF21B (ENSG00000116852), KIF17 (ENSG00000117245), KIF14 (ENSG00000118193), KIF18A (ENSG00000121621), KIF25 (ENSG00000125337), KIF1C (ENSG00000129250), KIF1A (ENSG00000130294), KIF3A (ENSG00000131437), KIF12 (ENSG00000136883), KIF13A (ENSG00000137177), KIF23 (ENSG00000137807), KIF11 (ENSG00000138160), CENPE (ENSG00000138778), KIF21A (ENSG00000139116), KIFC3 (ENSG00000140859), KIF2B (ENSG00000141200), KIF2C (ENSG00000142945), KIF5A (ENSG00000155980), KIF26B (ENSG00000162849), KIF15 (ENSG00000163808), KIF6 (ENSG00000164627), KIF27 (ENSG00000165115), KIF7 (ENSG00000166813), KIFC2 (ENSG00000167702), KIF5C (ENSG00000168280), KIF5B (ENSG00000170759), KIF18B (ENSG00000186185), KIF24 (ENSG00000186638), KIF19 (ENSG00000196169), KIF13B (ENSG00000197892)

Protein

Protein identifiers

Chromosome-associated kinesin KIF4B — Q2VIQ3 (reviewed: Q2VIQ3)

Alternative names: Chromokinesin-B

All UniProt accessions (1): Q2VIQ3

UniProt curated annotations — full annotation on UniProt →

Function. Iron-sulfur (Fe-S) cluster binding motor protein that has a role in chromosome segregation during mitosis. Translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization.

Subcellular location. Nucleus matrix. Cytoplasm. Cytoskeleton.

Tissue specificity. Specifically expressed in testis.

Cofactor. Binds 1 [4Fe-4S] cluster. In the presence of oxygen, the [4Fe-4S] cluster may be converted to [2Fe-2S].

Similarity. Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Chromokinesin subfamily.

RefSeq proteins (1): NP_001092763* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001752	Kinesin_motor_dom	Domain
IPR019821	Kinesin_motor_CS	Conserved_site
IPR027417	P-loop_NTPase	Homologous_superfamily
IPR027640	Kinesin-like_fam	Family
IPR033467	Tesmin/TSO1-like_CXC	Domain
IPR036961	Kinesin_motor_dom_sf	Homologous_superfamily

Pfam: PF00225, PF25764

UniProt features (49 total): sequence conflict 15, modified residue 12, region of interest 9, sequence variant 4, compositionally biased region 3, chain 1, domain 1, coiled-coil region 1, short sequence motif 1, binding site 1, cross-link 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q2VIQ3-F1	71.87	0.20

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (1): 88–95

Post-translational modifications (13): 394, 799, 801, 951, 1001, 1013, 1017, 1028, 1128, 1183, 1188, 1227, 1196

Function

Pathways and Gene Ontology

Reactome pathways

16 pathways

ID	Pathway
R-HSA-2132295	MHC class II antigen presentation
R-HSA-437239	Recycling pathway of L1
R-HSA-6811434	COPI-dependent Golgi-to-ER retrograde traffic
R-HSA-983189	Kinesins
R-HSA-109582	Hemostasis
R-HSA-1266738	Developmental Biology
R-HSA-1280218	Adaptive Immune System
R-HSA-168256	Immune System
R-HSA-199991	Membrane Trafficking
R-HSA-373760	L1CAM interactions
R-HSA-422475	Axon guidance
R-HSA-5653656	Vesicle-mediated transport
R-HSA-6811442	Intra-Golgi and retrograde Golgi-to-ER traffic
R-HSA-8856688	Golgi-to-ER retrograde transport
R-HSA-9675108	Nervous system development
R-HSA-983231	Factors involved in megakaryocyte development and platelet production

MSigDB gene sets: 72 (showing top): GSE45365_NK_CELL_VS_CD8_TCELL_UP, GOBP_MITOTIC_CYTOKINESIS, GOBP_CHROMOSOME_ORGANIZATION, REACTOME_ADAPTIVE_IMMUNE_SYSTEM, REACTOME_MEMBRANE_TRAFFICKING, GOBP_MITOTIC_SPINDLE_ASSEMBLY, GOBP_ORGANELLE_FISSION, GOBP_CYTOKINESIS, GOMF_CYTOSKELETAL_MOTOR_ACTIVITY, GOBP_MITOTIC_NUCLEAR_DIVISION, GOBP_ORGANELLE_ASSEMBLY, GOBP_MITOTIC_CELL_CYCLE, GOBP_SPINDLE_ASSEMBLY, GOBP_NUCLEAR_CHROMOSOME_SEGREGATION, GOBP_CYTOSKELETON_DEPENDENT_CYTOKINESIS

GO Biological Process (5): mitotic cytokinesis (GO:0000281), microtubule-based movement (GO:0007018), mitotic spindle organization (GO:0007052), spindle elongation (GO:0051231), mitotic spindle midzone assembly (GO:0051256)

GO Molecular Function (7): DNA binding (GO:0003677), microtubule motor activity (GO:0003777), ATP binding (GO:0005524), microtubule binding (GO:0008017), metal ion binding (GO:0046872), iron-sulfur cluster binding (GO:0051536), nucleotide binding (GO:0000166)

GO Cellular Component (7): cytosol (GO:0005829), microtubule (GO:0005874), microtubule associated complex (GO:0005875), nuclear matrix (GO:0016363), nucleus (GO:0005634), cytoplasm (GO:0005737), cytoskeleton (GO:0005856)

Reactome top-level categories

Rollup of top-12 pathways:

Category	Pathways
Adaptive Immune System	1
L1CAM interactions	1
Golgi-to-ER retrograde transport	1
Factors involved in megakaryocyte development and platelet production	1
Immune System	1
Vesicle-mediated transport	1
Axon guidance	1
Nervous system development	1
Membrane Trafficking	1
Intra-Golgi and retrograde Golgi-to-ER traffic	1
Developmental Biology	1
Hemostasis	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	3
mitotic cell cycle	2
mitotic cell cycle process	2
microtubule-based process	2
spindle organization	2
microtubule cytoskeleton	2
cytoskeleton-dependent cytokinesis	1
microtubule cytoskeleton organization involved in mitosis	1
cell cycle process	1
nuclear chromosome segregation	1
mitotic spindle elongation	1
spindle midzone assembly	1
mitotic spindle assembly	1
mitotic nuclear division	1
nucleic acid binding	1
cytoskeletal motor activity	1
polypeptide conformation or assembly isomerase activity	1
ATP-dependent activity	1
adenyl ribonucleotide binding	1
purine ribonucleoside triphosphate binding	1
tubulin binding	1
cation binding	1
metal cluster binding	1
nucleoside phosphate binding	1
heterocyclic compound binding	1
cytoplasm	1
polymeric cytoskeletal fiber	1
protein-containing complex	1
nuclear lumen	1
intracellular membrane-bounded organelle	1
intracellular anatomical structure	1
intracellular membraneless organelle	1

Protein interactions and networks

STRING

912 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
KIF4B	GMCL2	Q8NEA9	494
KIF4B	MAPRE1	Q15691	473
KIF4B	TRIP6	Q15654	435
KIF4B	ZNF784	Q8NCA9	413
KIF4B	AMDHD1	Q96NU7	370
KIF4B	KIF2C	Q99661	368
KIF4B	SKA3	Q8IX90	363
KIF4B	RAC1	P15154	349
KIF4B	PRC1	O43663	342
KIF4B	GMCL1	Q96IK5	340
KIF4B	KIF22	Q14807	335
KIF4B	DYNLT1	P63172	317
KIF4B	CDC14B	O60729	314
KIF4B	KIFAP3	Q92845	303
KIF4B	ZNF492	Q9P255	301

IntAct

12 interactions, top by confidence:

A	B	Type	Score
KIF4B	SMC2	psi-mi:“MI:0915”(physical association)	0.400
KIF4B	H2BC9	psi-mi:“MI:0915”(physical association)	0.400
HDAC2	KIF4B	psi-mi:“MI:0915”(physical association)	0.400
KIF4B	KIF4A	psi-mi:“MI:0915”(physical association)	0.400
Kif4	UMPS	psi-mi:“MI:0914”(association)	0.350
M		psi-mi:“MI:0914”(association)	0.350
S	IGLL5	psi-mi:“MI:0914”(association)	0.350
ARMC6	DDX39A	psi-mi:“MI:0914”(association)	0.350
SFN	DDX39A	psi-mi:“MI:0914”(association)	0.350
VENTX	UBA6	psi-mi:“MI:0914”(association)	0.350
SF3B1	FAM83G	psi-mi:“MI:0914”(association)	0.350

BioGRID (17): KIF4B (Co-fractionation), KIF4B (Affinity Capture-MS), HDAC2 (Proximity Label-MS), KIF4B (Proximity Label-MS), HIST1H2BH (Proximity Label-MS), KIF4B (Affinity Capture-MS), KIF4B (Proximity Label-MS), KIF4A (Affinity Capture-MS), KIF4B (Affinity Capture-MS), KIF4B (Proximity Label-MS), KIF4B (Affinity Capture-MS), KIF4B (Affinity Capture-MS), KIF4B (Reconstituted Complex), KIF4B (Affinity Capture-MS), STMN1 (Cross-Linking-MS (XL-MS))

ESM2 similar proteins: A0JN40, B1AVY7, B9F2Y7, F1M4A4, F1M5N7, F4K0J3, O14782, O15066, O35066, O55165, O60282, O75037, O95239, P17210, P21613, P28738, P33173, P33174, P33175, P33176, P34540, P56536, Q12756, Q12840, Q2PQA9, Q2VIQ3, Q498L9, Q58G59, Q5R706, Q5R9K7, Q61768, Q6P9L6, Q6QLM7, Q7M6Z4, Q7M6Z5, Q7TSP2, Q7Z4S6, Q86VH2, Q90640, Q91784

Diamond homologs: A0A068FIK2, A0JN40, A1ZAJ2, A8BB91, A8BKD1, B1AVY7, B7EJ91, B7ZNG0, B9F2Y7, B9GE13, F1M4A4, F1M5N7, F1QN54, F4IIS5, F4J1U4, F4K0J3, G5EGS3, O14343, O14782, O15066, O23826, O35066, O35071, O35787, O43896, O45935, O55165, O60282, O60333, O75037, O88658, O95239, P17210, P21613, P23678, P28738, P28741, P33173, P33174, P33175

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

194 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	167
Likely benign	13
Benign	14

Top pathogenic / likely-pathogenic (0)

SpliceAI

31 predictions. Top by Δscore:

Variant	Effect	Δscore
5:155013773:TTA:T	donor_gain	0.6100
5:155013866:G:GT	donor_gain	0.4400
5:155013821:G:T	donor_gain	0.4000
5:155013873:TG:T	donor_gain	0.3900
5:155013982:G:GA	donor_gain	0.3500
5:155014214:A:AG	donor_gain	0.3400
5:155013821:G:GT	donor_gain	0.3300
5:155013981:T:TA	donor_gain	0.3300
5:155013891:G:GT	donor_gain	0.3000
5:155014637:GGTAT:G	donor_gain	0.2900
5:155013794:G:GT	donor_gain	0.2800
5:155013886:T:G	donor_gain	0.2800
5:155013875:AAG:A	donor_gain	0.2700
5:155014641:T:TG	donor_gain	0.2700
5:155013769:GCGGT:G	donor_gain	0.2600
5:155013887:G:GG	donor_gain	0.2600
5:155013772:GT:G	donor_gain	0.2400
5:155013773:T:G	donor_gain	0.2300
5:155013801:G:GT	donor_gain	0.2200
5:155013983:GTG:G	donor_gain	0.2200
5:155013988:T:A	donor_gain	0.2200
5:155014764:G:GT	donor_gain	0.2200
5:155017439:GA:G	acceptor_gain	0.2200
5:155013815:AACGG:A	donor_gain	0.2100
5:155013822:A:T	donor_gain	0.2100
5:155013876:A:G	donor_gain	0.2100
5:155013889:AAG:A	donor_gain	0.2100
5:155013890:AGA:A	donor_gain	0.2100
5:155014635:A:T	donor_gain	0.2000
5:155017438:A:AG	acceptor_gain	0.2000

AlphaMissense

8200 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
5:155014121:G:T	G88W	0.998
5:155014122:G:A	G88E	0.998
5:155014584:C:A	A242D	0.998
5:155014659:T:C	L267P	0.998
5:155014799:A:C	S314R	0.998
5:155014801:T:A	S314R	0.998
5:155014801:T:G	S314R	0.998
5:155014122:G:T	G88V	0.997
5:155014140:A:T	K94I	0.997
5:155014440:G:A	G194D	0.997
5:155014581:T:C	L241P	0.997
5:155014587:G:A	G243E	0.997
5:155014637:G:C	G260R	0.997
5:155014730:T:C	S291P	0.997
5:155014848:C:A	A330D	0.997
5:155014121:G:A	G88R	0.996
5:155014121:G:C	G88R	0.996
5:155014439:G:C	G194R	0.996
5:155014586:G:A	G243R	0.996
5:155014586:G:C	G243R	0.996
5:155014651:C:A	N264K	0.996
5:155014651:C:G	N264K	0.996
5:155014668:T:C	L270S	0.996
5:155014725:G:C	R289T	0.996
5:155014725:G:T	R289I	0.996
5:155014726:A:C	R289S	0.996
5:155014726:A:T	R289S	0.996
5:155014743:G:C	R295P	0.996
5:155014791:C:A	A311D	0.996
5:155014116:C:A	A86D	0.995

dbSNP variants (sampled 300 via entrez): RS1000223141 (5:155017800 C>A), RS1001077102 (5:155011850 G>A), RS1001102102 (5:155012565 G>A), RS1001731540 (5:155018372 C>A,G,T), RS1003572806 (5:155016147 A>G), RS1004932391 (5:155017120 G>T), RS1005061512 (5:155015779 A>G), RS1005279093 (5:155015538 A>G), RS1005342836 (5:155015852 A>G), RS1005398272 (5:155017416 C>T), RS10056252 (5:155015910 A>G), RS1006951262 (5:155012626 T>C), RS1007290681 (5:155013220 T>C), RS1007995188 (5:155018310 G>A,T), RS1008594323 (5:155017850 A>G)

Disease associations

OMIM: gene MIM:609184 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

4 associations (top):

Study	Trait	p-value
GCST002976_6	HIV-1 viral setpoint	8.000000e-06
GCST005782_2	Serum 25-Hydroxyvitamin D levels	3.000000e-08
GCST011116_7	Coronary artery disease in type 1 diabetes	9.000000e-07
GCST011703_72	Smoking initiation	2.000000e-09

EFO canonical traits (2, from GWAS)

EFO ID	Trait name
EFO:0006319	HIV viral set point measurement
EFO:0005670	smoking initiation

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

8 total (human), top 8 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
bisphenol A	decreases expression	1
2,2’,4,4’-tetrabromodiphenyl ether	affects expression	1
theaflavin-3,3’-digallate	affects expression	1
Resveratrol	affects cotreatment, decreases expression	1
Benzo(a)pyrene	decreases methylation	1
Copper	affects cotreatment, decreases expression	1
Lucanthone	decreases expression	1
Urethane	increases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.