LRWD1

Summary

LRWD1 (leucine rich repeats and WD repeat domain containing 1, HGNC:21769) is a protein-coding gene on chromosome 7q22.1, encoding Leucine-rich repeat and WD repeat-containing protein 1 (Q9UFC0). Required for G1/S transition.

The protein encoded by this gene interacts with components of the origin recognition complex (ORC) and regulates the formation of the prereplicative complex. The encoded protein stabilizes the ORC and therefore aids in DNA replication. This protein is required for the G1/S phase transition of the cell cycle. In addition, the encoded protein binds to trimethylated histone H3 in heterochromatin and recruits the ORC and lysine methyltransferases, which help maintain the repressive heterochromatic state. Two transcript variants encoding different isoforms have been found for this gene.

Source: NCBI Gene 222229 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 143 total
• Druggable target: yes
• MANE Select transcript: NM_152892

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:21769
Approved symbol	LRWD1
Name	leucine rich repeats and WD repeat domain containing 1
Location	7q22.1
Locus type	gene with protein product
Status	Approved
Aliases	DKFZp434K1815, ORCA, CENP-33
Ensembl gene	ENSG00000161036
Ensembl biotype	protein_coding
OMIM	615167
Entrez	222229

Gene structure

Transcript identifiers

Ensembl transcripts: 18 — 13 protein_coding, 3 nonsense_mediated_decay, 2 retained_intron

ENST00000292616, ENST00000463739, ENST00000464107, ENST00000468175, ENST00000473880, ENST00000476270, ENST00000485417, ENST00000485808, ENST00000626402, ENST00000896258, ENST00000896259, ENST00000896260, ENST00000896261, ENST00000896262, ENST00000896263, ENST00000922655, ENST00000922656, ENST00000965088

RefSeq mRNA: 2 — MANE Select: NM_152892 NM_001317721, NM_152892

CCDS: CCDS34715

Canonical transcript exons

ENST00000292616 — 15 exons

Exon	Start	End
ENSE00001056068	102472218	102472309
ENSE00001056070	102469742	102469882
ENSE00001056077	102472454	102472609
ENSE00001056079	102468554	102468654
ENSE00001056082	102468855	102469062
ENSE00001133591	102472909	102473168
ENSE00001133598	102464984	102465160
ENSE00003455861	102472692	102472804
ENSE00003461283	102469574	102469646
ENSE00003500614	102466154	102466270
ENSE00003510614	102465817	102466051
ENSE00003621584	102467339	102467479
ENSE00003669752	102468062	102468187
ENSE00003670699	102467719	102467823
ENSE00003789422	102468263	102468377

Expression profiles

Bgee: expression breadth ubiquitous, 235 present calls, max score 99.15.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 19.3848 / max 802.4802, expressed in 1794 samples.

FANTOM5 promoters (1 alternative TSS)

Promoter ID	TPM avg	Samples expressed
80256	19.3848	1794

Top tissues by expression

248 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
left testis	UBERON:0004533	99.15	gold quality
right testis	UBERON:0004534	99.01	gold quality
testis	UBERON:0000473	96.76	gold quality
adult organism	UBERON:0007023	95.19	gold quality
right uterine tube	UBERON:0001302	92.93	gold quality
nasal cavity epithelium	UBERON:0005384	92.47	silver quality
cerebellar vermis	UBERON:0004720	91.94	gold quality
cortical plate	UBERON:0005343	90.99	gold quality
oocyte	CL:0000023	90.55	gold quality
right hemisphere of cerebellum	UBERON:0014890	90.41	gold quality
cerebellar cortex	UBERON:0002129	90.02	gold quality
cerebellar hemisphere	UBERON:0002245	90.02	gold quality
cerebellum	UBERON:0002037	89.80	gold quality
bronchial epithelial cell	CL:0002328	89.39	gold quality
vena cava	UBERON:0004087	89.27	silver quality
bronchus	UBERON:0002185	89.24	gold quality
parotid gland	UBERON:0001831	89.05	silver quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	88.80	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	88.71	gold quality
blood	UBERON:0000178	88.57	gold quality
sperm	CL:0000019	88.55	gold quality
lateral nuclear group of thalamus	UBERON:0002736	88.20	gold quality
spleen	UBERON:0002106	87.93	gold quality
granulocyte	CL:0000094	87.90	gold quality
secondary oocyte	CL:0000655	87.67	gold quality
right adrenal gland	UBERON:0001233	87.27	gold quality
pituitary gland	UBERON:0000007	87.25	gold quality
kidney epithelium	UBERON:0004819	87.23	gold quality
left adrenal gland	UBERON:0001234	86.92	gold quality
left adrenal gland cortex	UBERON:0035825	86.92	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 2.

Experiment	Marker?	Max mean expression
E-GEOD-134144	yes	1915.71
E-ANND-3	yes	5.98

Regulation

Is transcription factor: no

Upstream regulators (CollecTRI, top): NFKB

miRNA regulators (miRDB)

6 targeting LRWD1, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-4420	99.82	70.08	1624
HSA-MIR-181B-2-3P	99.81	70.06	1646
HSA-MIR-181B-3P	99.81	70.06	1646
HSA-MIR-4427	99.34	70.33	1854
HSA-MIR-3121-5P	97.30	66.62	1146
HSA-MIR-6075	93.03	64.73	45

Literature-anchored findings (GeneRIF, showing 12)

• Results suggest ORCA-mediated association of ORC to chromatin is critical to initiate preRC assembly in G1 and chromatin organization in post-G1 cells. (PMID:20932478)
• Leucine-rich repeat and WD repeat-containing protein 1 is recruited to pericentric heterochromatin by trimethylated lysine 9 of histone H3 and maintains heterochromatin silencing (PMID:22427655)
• propose that the dynamic association of ORCA with pre-RC components modulates the assembly of its interacting partners on chromatin and facilitates DNA replication initiation (PMID:22645314)
• Orc2 protects ORCA from ubiquitin-mediated degradation in vivo. (PMID:22935713)
• This is the first study showing that the expression of the testis-enriched LRWD1 gene is regulated by NF-kappaB. (PMID:23275029)
• three coding single-nucleotide polymorphisms (SNP1-SNP3) could be detected in Japanese patients with Sertoli cell-only syndrome (PMID:23445371)
• Data (from studies using recombinant proteins) suggest that deletion of leucine rich (LRR) domain significantly down-regulates expression of LRWD1 protein; LRR domain is significantly correlated with stability of LRWD1 protein. (PMID:25170821)
• Data show that ORCA acts as a scaffold for the establishment of H3K9 KMT complex. (PMID:25922909)
• LRWD1 is a novel regulator of EWS-FLI1 driven cell proliferation in Ewing sarcoma cells. EWS-FLI1 regulates LRWD1 expression and LRWD1 may contribute to EWS-FLI1 mediated transcriptional repression. (PMID:27760381)
• a novel function of LRWD1 in controlling microtubule nucleation and cell cycle progression in the human testicular embryonic carcinoma cells. (PMID:28569402)
• Nuclear factor erythroid-2-related factor regulates LRWD1 expression and cellular adaptation to oxidative stress in human embryonal carcinoma cells (PMID:29544732)
• MicroRNA-320a enhances LRWD1 expression through the AGO2/FXR1-dependent pathway to affect cell behaviors and the oxidative stress response in human testicular embryonic carcinoma cells. (PMID:38385979)

Cross-species orthologs

4 orthologs

Organism	Symbol	Gene ID
danio_rerio	lrwd1	ENSDARG00000035147
mus_musculus	Lrwd1	ENSMUSG00000029703
rattus_norvegicus	Lrwd1	ENSRNOG00000001429
caenorhabditis_elegans		WBGENE00019351

Paralogs (1): OPTC (ENSG00000188770)

Protein

Protein identifiers

Leucine-rich repeat and WD repeat-containing protein 1 — Q9UFC0 (reviewed: Q9UFC0)

Alternative names: Centromere protein 33, Origin recognition complex-associated protein

All UniProt accessions (7): Q9UFC0, A0A140VJD0, F8WBP5, F8WDB4, H7C4Q9, H7C5N7, H7C5S6

UniProt curated annotations — full annotation on UniProt →

Function. Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability.

Subunit / interactions. Integral component of the ORC complex. Directly interacts with CDT1, GMNN and ORC2. Interacts with ORC2 only when non-ubiquitinated; this interaction prevents LRWD1 ubiquitination and degradation. Some of these interactions are regulated in a cell-cycle dependent manner. Interaction with ORC1 occurs predominantly during G1. Association with phosphorylated ORC1 during mitosis is not efficient. Interaction with CDT1 occurs during G1 phase, as well as during mitosis with phosphorylated CDT1. Interaction with GMNN occurs from G1/S to mitosis. Interaction with ORC2 is observed throughout the cell cycle. The stoichiometry of the ORCA/ORC/CDT1/GMNN complex is 1:1:1:2. Interacts with CUL4A and DDB1; this interaction may lead to ubiquitination.

Subcellular location. Nucleus. Chromosome. Centromere. Telomere. Cytoplasm. Cytoskeleton. Microtubule organizing center. Centrosome. Kinetochore.

Tissue specificity. Testis-specific. Drastically down-regulated in testis from patients with Sertoli cell-only syndrome (SCOS).

Post-translational modifications. Ubiquitinated; undergoes ‘Lys-48’-linked polyubiquitination leading to proteasomal degradation. Ubiquitination occurs within the WD repeats at the end of the G1 phase. Ubiquitination may be catalyzed by the CUL4-DDB1 E3 ubiquitin-protein ligase complex and other E3 ligases.

Domain organisation. The entire WD repeat region is required for the interaction with ORC, CDT1 and GMNN, as well as for association with chromatin and for binding to histone H3 and H4 trimethylation marks H3K9me3 and H4K20me3.

Similarity. Belongs to the LRWD1 family.

RefSeq proteins (2): NP_001304650, NP_690852* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR001611	Leu-rich_rpt	Repeat
IPR001680	WD40_rpt	Repeat
IPR003591	Leu-rich_rpt_typical-subtyp	Repeat
IPR015943	WD40/YVTN_repeat-like_dom_sf	Homologous_superfamily
IPR019775	WD40_repeat_CS	Conserved_site
IPR032675	LRR_dom_sf	Homologous_superfamily
IPR036322	WD40_repeat_dom_sf	Homologous_superfamily
IPR052489	LRWD1	Family
IPR056160	WD_LRWD1	Domain
IPR056363	LRR_LRWD1_dom	Domain

Pfam: PF23211, PF23215

UniProt features (19 total): repeat 11, modified residue 5, chain 1, region of interest 1, sequence conflict 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q9UFC0-F1	84.15	0.61

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Post-translational modifications (5): 212, 243, 251, 259, 264

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 136 (showing top): RRAGTTGT_UNKNOWN, GOBP_ESTABLISHMENT_OF_PROTEIN_LOCALIZATION_TO_CHROMOSOME, GRAESSMANN_APOPTOSIS_BY_DOXORUBICIN_UP, GOBP_ESTABLISHMENT_OF_PROTEIN_LOCALIZATION_TO_ORGANELLE, GOCC_MICROTUBULE_ORGANIZING_CENTER, GOCC_CENTROSOME, GOBP_DNA_REPLICATION, ACTWSNACTNY_UNKNOWN, GOMF_CHROMATIN_BINDING, GOCC_CHROMOSOMAL_REGION, GOCC_PERICENTRIC_HETEROCHROMATIN, GOCC_CONDENSED_CHROMOSOME_CENTROMERIC_REGION, GOCC_HETEROCHROMATIN, GOCC_CHROMOSOME_TELOMERIC_REGION, GOCC_NUCLEOLUS

GO Biological Process (4): DNA replication initiation (GO:0006270), chromatin organization (GO:0006325), establishment of protein localization to chromatin (GO:0071169), DNA replication (GO:0006260)

GO Molecular Function (4): chromatin binding (GO:0003682), methyl-CpG binding (GO:0008327), histone H3K27me3 reader activity (GO:0061628), protein binding (GO:0005515)

GO Cellular Component (12): kinetochore (GO:0000776), chromosome, telomeric region (GO:0000781), nucleus (GO:0005634), nucleoplasm (GO:0005654), nuclear origin of replication recognition complex (GO:0005664), pericentric heterochromatin (GO:0005721), nucleolus (GO:0005730), centrosome (GO:0005813), chromosome, centromeric region (GO:0000775), chromosome (GO:0005694), cytoplasm (GO:0005737), cytoskeleton (GO:0005856)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
intracellular membraneless organelle	4
DNA metabolic process	2
binding	2
chromosomal region	2
nuclear lumen	2
cellular anatomical structure	2
DNA-templated DNA replication	1
cellular component organization	1
establishment of protein localization to chromosome	1
DNA biosynthetic process	1
nucleotide binding	1
sequence-specific DNA binding	1
histone H3 reader activity	1
condensed chromosome, centromeric region	1
supramolecular complex	1
intracellular membrane-bounded organelle	1
nuclear chromosome	1
origin recognition complex	1
nuclear protein-containing complex	1
chromosome, centromeric region	1
heterochromatin	1
centriole	1
microtubule organizing center	1
intracellular anatomical structure	1

Protein interactions and networks

STRING

1092 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
LRWD1	ORC3	Q9UBD5	732
LRWD1	CDT1	Q9H211	703
LRWD1	ORC4	O43929	685
LRWD1	ORC5	O43913	683
LRWD1	TICRR	Q7Z2Z1	562
LRWD1	CLBA1	Q96F83	541
LRWD1	ORC2	Q13416	525
LRWD1	OBI1	Q5W0B1	518
LRWD1	KMT5A	Q9NQR1	509
LRWD1	ASPH	Q12797	506
LRWD1	VWA5B2	Q8N398	483
LRWD1	ORC6	Q9Y5N6	436
LRWD1	NAP1L4	Q99733	433
LRWD1	PHIP	Q8WWQ0	422
LRWD1	KIF9	Q9HAQ2	421

IntAct

51 interactions, top by confidence:

A	B	Type	Score
ORC2	ORC5	psi-mi:“MI:0914”(association)	0.810
DNAJC7	PLD2	psi-mi:“MI:0914”(association)	0.640
RPGR	NPHP1	psi-mi:“MI:0914”(association)	0.560
STN1	SMCO3	psi-mi:“MI:0914”(association)	0.530
ORC6	DCTN6	psi-mi:“MI:0914”(association)	0.530
H3C1	SMCHD1	psi-mi:“MI:2364”(proximity)	0.410
Orc1	ORC5	psi-mi:“MI:0915”(physical association)	0.400
	LRWD1	psi-mi:“MI:0915”(physical association)	0.400
LRWD1	NUDC	psi-mi:“MI:0915”(physical association)	0.400
Cbx1		psi-mi:“MI:0914”(association)	0.350
Orc2	FAM210A	psi-mi:“MI:0914”(association)	0.350
MPHOSPH8	HCFC1	psi-mi:“MI:0914”(association)	0.350
LRWD1	ORC5	psi-mi:“MI:0914”(association)	0.350
ORC1	ZNF768	psi-mi:“MI:0914”(association)	0.350
FOXB1	DDX39A	psi-mi:“MI:0914”(association)	0.350
FOXE1	DDX39A	psi-mi:“MI:0914”(association)	0.350
FOXG1	DDX39A	psi-mi:“MI:0914”(association)	0.350
FOXM1	SMARCA5	psi-mi:“MI:0914”(association)	0.350
FOXQ1	DDX39A	psi-mi:“MI:0914”(association)	0.350
FOXS1	DDX39A	psi-mi:“MI:0914”(association)	0.350
TEAD2	DDX39A	psi-mi:“MI:0914”(association)	0.350
ESR1	ESYT2	psi-mi:“MI:0914”(association)	0.350
LYPD4	DPYSL4	psi-mi:“MI:0914”(association)	0.350
RPGR	NPHP1	psi-mi:“MI:0914”(association)	0.350
RNF126	CAPN15	psi-mi:“MI:0914”(association)	0.350
ENG	IGKV2-28	psi-mi:“MI:0914”(association)	0.350

BioGRID (171): LRWD1 (Protein-peptide), LRWD1 (Protein-peptide), LRWD1 (Affinity Capture-MS), LRWD1 (Affinity Capture-MS), LRWD1 (Affinity Capture-MS), LRWD1 (Affinity Capture-MS), LRWD1 (Affinity Capture-MS), LRWD1 (Affinity Capture-MS), LRWD1 (Affinity Capture-MS), LRWD1 (Affinity Capture-MS), LRWD1 (Affinity Capture-MS), LRWD1 (Co-fractionation), LRWD1 (Co-fractionation), LRWD1 (Affinity Capture-MS), ADD1 (Affinity Capture-MS)

ESM2 similar proteins: A0JMH2, A1L251, A2ARP1, A7Z050, D3ZEY4, E7FCP8, E9QAM5, O00562, O35954, O42412, O95822, P0C644, P0C7A1, P12617, P16386, P40935, P49898, P49899, P52824, P53370, P54310, P70563, Q17QN2, Q2KI24, Q3URQ7, Q499U8, Q5EU90, Q5I0I8, Q5RAR6, Q5RDF1, Q5TGY1, Q5U2N3, Q5XIL6, Q68J42, Q6P5E8, Q6PD24, Q6PFW1, Q80YU0, Q8BX80, Q8CH40

Diamond homologs: B0JZ65, B0R160, Q6GM71, Q8BUI3, Q9UFC0, E3LB80, O16023, O43071, O75530, Q24338, Q26458, Q28DT7, Q2KJJ5, Q3SZ25, Q54JS5, Q54QU5, Q566T0, Q5I0B9, Q5U2W5, Q5ZKH3, Q676U5, Q6AZS2, Q6ZJW8, Q6ZJX0, Q756D0, Q7KWL3, Q8C4J7, Q8UUP2, Q8VZY6, Q8VZY7, Q921E6, Q9CWU9, Q9LT47, Q9NV06, U4PCM1, O22468, O60508, Q498F0, Q5JSH3, Q6NVE8

SIGNOR signaling

0 interactions.

Enriched among interaction partners

Reactome pathways and GO biological processes over-represented among this gene’s 67 IntAct physical interaction partners (hypergeometric vs the genome-wide background, BH-FDR, gene-set size 15–500, ranked by fold). A functional readout of the neighbourhood — distinct from this gene’s own memberships above, and biased toward well-studied / hub proteins, so read it as themes rather than proof.

Reactome pathways:

Pathway	Partners	Fold	FDR
Assembly of the ORC complex at the origin of replication	6	22.6×	2e-05
Orc1 removal from chromatin	5	20.3×	3e-04
Assembly of the pre-replicative complex	5	15.8×	6e-04

Disease & clinical

Clinical variants and AI predictions

ClinVar

143 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	118
Likely benign	7
Benign	1

Top pathogenic / likely-pathogenic (0)

SpliceAI

2677 predictions. Top by Δscore:

Variant	Effect	Δscore
7:102465143:G:GT	donor_gain	1.0000
7:102465143:G:T	donor_gain	1.0000
7:102465158:G:GT	donor_gain	1.0000
7:102465158:GGA:G	donor_gain	1.0000
7:102465159:GA:G	donor_gain	1.0000
7:102465159:GAG:G	donor_gain	1.0000
7:102465161:G:GG	donor_gain	1.0000
7:102465815:A:AG	acceptor_gain	1.0000
7:102465815:AGCCT:A	acceptor_gain	1.0000
7:102465816:G:GA	acceptor_gain	1.0000
7:102465816:GC:G	acceptor_gain	1.0000
7:102465816:GCC:G	acceptor_gain	1.0000
7:102465816:GCCT:G	acceptor_gain	1.0000
7:102465816:GCCTG:G	acceptor_gain	1.0000
7:102466047:TGACG:T	donor_gain	1.0000
7:102466048:GACG:G	donor_gain	1.0000
7:102466048:GACGG:G	donor_gain	1.0000
7:102466049:ACG:A	donor_gain	1.0000
7:102466050:CG:C	donor_gain	1.0000
7:102466050:CGGTA:C	donor_loss	1.0000
7:102466051:GG:G	donor_gain	1.0000
7:102466052:G:GG	donor_gain	1.0000
7:102466052:GT:G	donor_loss	1.0000
7:102466149:CCCA:C	acceptor_loss	1.0000
7:102466150:CCAG:C	acceptor_loss	1.0000
7:102466151:CAG:C	acceptor_loss	1.0000
7:102466152:A:AC	acceptor_loss	1.0000
7:102466152:A:AG	acceptor_gain	1.0000
7:102466153:G:GG	acceptor_gain	1.0000
7:102466153:G:GT	acceptor_loss	1.0000

AlphaMissense

4179 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
7:102468346:G:C	W296C	0.998
7:102468346:G:T	W296C	0.998
7:102468575:C:A	A314D	0.998
7:102468873:T:A	W347R	0.998
7:102468873:T:C	W347R	0.998
7:102468344:T:A	W296R	0.997
7:102468344:T:C	W296R	0.997
7:102469057:T:C	L408P	0.997
7:102469603:T:A	W420R	0.997
7:102469603:T:C	W420R	0.997
7:102472564:T:A	W549R	0.997
7:102472564:T:C	W549R	0.997
7:102468955:T:A	V374D	0.996
7:102472489:T:A	W524R	0.996
7:102472489:T:C	W524R	0.996
7:102468356:T:C	F300L	0.995
7:102468358:C:A	F300L	0.995
7:102468358:C:G	F300L	0.995
7:102472461:G:C	K514N	0.995
7:102472461:G:T	K514N	0.995
7:102472918:T:A	W605R	0.995
7:102472918:T:C	W605R	0.995
7:102465891:T:C	L52P	0.994
7:102466023:T:C	L96P	0.994
7:102468574:G:C	A314P	0.994
7:102469057:T:A	L408H	0.994
7:102469574:C:A	T410K	0.994
7:102465957:T:C	L74P	0.993
7:102466014:T:A	L93H	0.993
7:102466039:C:A	N101K	0.993

dbSNP variants (sampled 300 via entrez): RS1000046552 (7:102463744 T>C), RS1000182471 (7:102469169 C>T), RS1000515753 (7:102468149 G>A), RS1000594052 (7:102468009 G>A), RS1000654546 (7:102473124 G>A,C,T), RS1001262173 (7:102466472 T>C), RS1001262205 (7:102469986 G>A,C,T), RS1001361022 (7:102473426 G>A), RS1002196579 (7:102471759 T>C), RS1002390196 (7:102465153 A>C,T), RS1002417126 (7:102471665 G>A,C), RS1002981323 (7:102467942 C>A,T), RS1003029816 (7:102463670 G>A), RS1003054432 (7:102467718 G>C,T), RS1003318430 (7:102466666 C>A,T)

Disease associations

OMIM: gene MIM:615167 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL5724638 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

28 total (human), top 28 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Air Pollutants	affects expression, increases abundance, increases expression	2
Benzo(a)pyrene	affects methylation, increases expression	2
Smoke	decreases expression, increases abundance, increases expression	2
aristolochic acid I	increases expression	1
FR900359	affects phosphorylation	1
triphenyl phosphate	affects expression	1
bisphenol A	decreases expression	1
sodium arsenite	increases expression	1
cupric chloride	increases expression	1
coumarin	affects phosphorylation	1
Grape Seed Proanthocyanidins	decreases expression, affects cotreatment	1
bisphenol S	affects cotreatment, decreases expression	1
Acetaminophen	increases expression	1
Caffeine	increases phosphorylation	1
Catechin	affects cotreatment, decreases expression	1
Dexamethasone	affects cotreatment, decreases expression	1
Indomethacin	affects cotreatment, decreases expression	1
Ivermectin	decreases expression	1
Ozone	affects expression, increases abundance	1
Rotenone	decreases expression	1
Tobacco Smoke Pollution	decreases expression	1
Urethane	increases expression	1
Valproic Acid	increases expression, increases methylation	1
1-Methyl-3-isobutylxanthine	affects cotreatment, decreases expression	1
Cyclosporine	increases expression	1
Okadaic Acid	increases expression	1
Copper Sulfate	increases expression	1
Vitamin K 3	affects expression	1

ChEMBL screening assays

6 unique, capped per target: 6 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL5697377	Binding	Inhibition of LRWD1 (unknown origin) assessed as fold change at 10 uM incubated for 1 hr by colloidal coomassie staining based LC-MS/MS analysis	Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukaemia. — Nature

Cellosaurus cell lines

3 cell lines: 3 cancer cell line

First 10 cell lines (id-ordered, not curated):

Cellosaurus	Name	Category	Sex
CVCL_B1W7	Abcam HeLa LRWD1 KO	Cancer cell line	Female
CVCL_E2BL	HAP1 LRWD1 (-) 1	Cancer cell line	Male
CVCL_E2BM	HAP1 LRWD1 (-) 2	Cancer cell line	Male

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.