MAGEA10

Summary

MAGEA10 (MAGE family member A10, HGNC:6797) is a protein-coding gene on chromosome Xq28, encoding Melanoma-associated antigen 10 (P43363). Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream melanoma antigen family A, 5 (MAGEA5) gene.

Source: NCBI Gene 4109 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 1 total
• MANE Select transcript: NM_021048

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:6797
Approved symbol	MAGEA10
Name	MAGE family member A10
Location	Xq28
Locus type	gene with protein product
Status	Approved
Aliases	MGC10599, CT1.10
Ensembl gene	ENSG00000124260
Ensembl biotype	protein_coding
OMIM	300343
Entrez	4109

Gene structure

Transcript identifiers

Ensembl transcripts: 6 — 6 protein_coding

ENST00000244096, ENST00000370323, ENST00000427322, ENST00000444834, ENST00000579960, ENST00000583480

RefSeq mRNA: 3 — MANE Select: NM_021048 NM_001011543, NM_001251828, NM_021048

CCDS: CCDS14705

Canonical transcript exons

ENST00000370323 — 4 exons

Exon	Start	End
ENSE00001310504	152138475	152138577
ENSE00001840920	152133310	152135685
ENSE00003625659	152136871	152136969
ENSE00003673069	152135761	152135834

Expression profiles

Bgee: expression breadth broad, 18 present calls, max score 80.66.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 0.5643 / max 130.3082, expressed in 26 samples.

FANTOM5 promoters (3 alternative TSS)

Promoter ID	TPM avg	Samples expressed
200844	0.4556	24
200842	0.0729	20
200843	0.0358	16

Top tissues by expression

260 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	80.66	gold quality
placenta	UBERON:0001987	78.61	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	77.67	gold quality
endometrium epithelium	UBERON:0004811	63.86	gold quality
CA1 field of hippocampus	UBERON:0003881	59.17	gold quality
decidua	UBERON:0002450	58.44	gold quality
tongue squamous epithelium	UBERON:0006919	58.38	gold quality
right testis	UBERON:0004534	56.07	gold quality
vena cava	UBERON:0004087	55.73	gold quality
hair follicle	UBERON:0002073	55.17	gold quality
skeletal muscle tissue of biceps brachii	UBERON:0004502	54.99	gold quality
testis	UBERON:0000473	54.76	gold quality
vastus lateralis	UBERON:0001379	54.30	gold quality
cerebellar vermis	UBERON:0004720	53.98	gold quality
quadriceps femoris	UBERON:0001377	53.95	gold quality
tibia	UBERON:0000979	53.70	gold quality
sperm	CL:0000019	53.57	gold quality
male germ cell	CL:0000015	53.56	gold quality
left testis	UBERON:0004533	53.32	gold quality
metanephric glomerulus	UBERON:0004736	52.27	gold quality
parotid gland	UBERON:0001831	50.91	gold quality
oral cavity	UBERON:0000167	50.68	gold quality
frontal pole	UBERON:0002795	50.41	gold quality
middle frontal gyrus	UBERON:0002702	50.30	gold quality
paraflocculus	UBERON:0005351	50.18	gold quality
Brodmann (1909) area 10	UBERON:0013541	50.18	gold quality
cardiac muscle of right atrium	UBERON:0003379	49.95	gold quality
kidney epithelium	UBERON:0004819	49.60	gold quality
myocardium	UBERON:0002349	49.51	gold quality
thymus	UBERON:0002370	49.33	gold quality

Single-cell (SCXA)

Detected in 2 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-MTAB-7249	yes	10.91
E-ANND-3	no	2.30

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

47 targeting MAGEA10, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-5692A	100.00	74.40	6850
HSA-MIR-3185	99.99	68.12	1959
HSA-MIR-196A-1-3P	99.99	72.15	2772
HSA-MIR-4500	99.99	72.72	2367
HSA-MIR-4775	99.98	75.00	6394
HSA-MIR-5696	99.98	72.36	4487
HSA-MIR-3692-3P	99.98	70.27	2139
HSA-MIR-4803	99.98	71.99	3117
HSA-MIR-4666A-3P	99.96	71.71	3434
HSA-MIR-128-3P	99.95	71.17	2484
HSA-MIR-216A-3P	99.95	71.19	2505
HSA-MIR-335-3P	99.93	73.36	4958
HSA-MIR-338-5P	99.92	72.34	2951
HSA-MIR-3681-3P	99.88	70.46	2254
HSA-MIR-548AR-3P	99.85	71.26	3889
HSA-MIR-548AZ-3P	99.82	70.56	3549
HSA-MIR-548BC	99.82	70.61	3524
HSA-MIR-548E-3P	99.82	70.59	3514
HSA-MIR-548F-3P	99.82	70.59	3540
HSA-MIR-4760-5P	99.80	69.88	1619
HSA-MIR-548A-3P	99.76	70.58	3524
HSA-MIR-8084	99.73	69.57	1760
HSA-MIR-4729	99.69	72.18	4233
HSA-MIR-6516-3P	99.65	68.57	1238
HSA-MIR-582-5P	99.47	70.79	2635
HSA-MIR-580-5P	99.28	70.94	1776
HSA-MIR-2054	99.20	68.89	1699
HSA-MIR-10399-5P	99.17	69.87	2610
HSA-MIR-6504-3P	99.17	69.31	2891
HSA-MIR-628-3P	99.04	68.37	814

Literature-anchored findings (GeneRIF, showing 8)

• This is the first report of these MAGE-A10 alternative splice sequences, and characterization of MAGE-A expression (PMID:15355897)
• High MAGE-A10 is associated with lung, skin and urothelial malignancies. (PMID:21710496)
• We clearly showed that MAGE-A10 is frequently expressed in the group of triple-negative breast cancer patients (PMID:22116775)
• By using Molecular-Docking, 3 MAGEA10 peptides were found binding to the groove position of HLA-A( *)0210 same as MAGEA4 peptide co-crystallized with HLA-A( *)0210, which indicates they could be promising for HLA-A( *)0201 presentation in immunotherapy. (PMID:26058806)
• CTAs (MAGE-A4, NY-ESO-1, MAGE-A10) were more likely expressed in patients with squamous cell carcinoma of the lung and when CTAs combined with CD133, they can be better prognostic factors. (PMID:26191258)
• MAGEA10 expression is a predictive marker of early hepatic recurrence after curative gastrectomy for gastric and gastroesophageal junction cancer. (PMID:32965606)
• How the Intrinsically Disordered N-Terminus of Cancer/Testis Antigen MAGEA10 Is Responsible for Its Expression, Nuclear Localisation and Aberrant Migration. (PMID:38136576)
• Expression of MAGE A1 to MAGE A10 in the Forceps Biopsy and Bronchoalveolar Lavage Specimens from Patients with the Central Lung Tumor. (PMID:38285780)

Cross-species orthologs

20 orthologs

Organism	Symbol	Gene ID
danio_rerio	ndnl2	ENSDARG00000058212
mus_musculus	Magea14	ENSMUSG00000031118
mus_musculus	Magea9	ENSMUSG00000031179
mus_musculus	Magea4	ENSMUSG00000033343
mus_musculus	Magea10	ENSMUSG00000043453
mus_musculus	Magea2	ENSMUSG00000055746
mus_musculus	Magea6	ENSMUSG00000063728
mus_musculus	Magea5	ENSMUSG00000079349
mus_musculus	Magea8	ENSMUSG00000079350
mus_musculus	Magea3	ENSMUSG00000094196
mus_musculus	Magea1	ENSMUSG00000096644
rattus_norvegicus	Magea9	ENSRNOG00000023016
rattus_norvegicus	Magea14	ENSRNOG00000032629
rattus_norvegicus	Magea10	ENSRNOG00000052773
rattus_norvegicus	Magea10-ps3	ENSRNOG00000062420
rattus_norvegicus		ENSRNOG00000073163
rattus_norvegicus		ENSRNOG00000075783
rattus_norvegicus		ENSRNOG00000080175
rattus_norvegicus		ENSRNOG00000082604
drosophila_melanogaster	MAGE	FBGN0037481

Paralogs (37): MAGEC2 (ENSG00000046774), TRO (ENSG00000067445), MAGEB2 (ENSG00000099399), MAGED2 (ENSG00000102316), MAGEB4 (ENSG00000120289), MAGEA9 (ENSG00000123584), MAGEA4 (ENSG00000147381), MAGED4 (ENSG00000154545), MAGEC1 (ENSG00000155495), MAGEA8 (ENSG00000156009), MAGEC3 (ENSG00000165509), MAGEB6 (ENSG00000176746), MAGEB18 (ENSG00000176774), MAGEF1 (ENSG00000177383), MAGEB10 (ENSG00000177689), MAGED1 (ENSG00000179222), NDN (ENSG00000182636), MAGEB17 (ENSG00000182798), MAGEA2B (ENSG00000183305), NSMCE3 (ENSG00000185115), MAGEA11 (ENSG00000185247), MAGEE2 (ENSG00000186675), MAGED4B (ENSG00000187243), MAGEH1 (ENSG00000187601), MAGEB5 (ENSG00000188408), MAGEB16 (ENSG00000189023), MAGEA6 (ENSG00000197172), MAGEA1 (ENSG00000198681), MAGEB3 (ENSG00000198798), MAGEE1 (ENSG00000198934), MAGEA12 (ENSG00000213401), MAGEB1 (ENSG00000214107), MAGEA3 (ENSG00000221867), MAGEB6B (ENSG00000232030), MAGEL2 (ENSG00000254585), MAGEA9B (ENSG00000267978), MAGEA2 (ENSG00000268606)

Protein

Protein identifiers

Melanoma-associated antigen 10 — P43363 (reviewed: P43363)

Alternative names: Cancer/testis antigen 1.10, MAGE-10 antigen

All UniProt accessions (5): C9J958, C9J9A2, P43363, J3KT71, J3QRD7

UniProt curated annotations — full annotation on UniProt →

Function. Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.

Subcellular location. Nucleus.

Tissue specificity. Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for spermatogonia, spermatocytes and placenta.

RefSeq proteins (3): NP_001011543, NP_001238757, NP_066386* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR002190	MHD_dom	Domain
IPR021072	MAGE_N	Domain
IPR037445	MAGE	Family
IPR041898	MAGE_WH1	Homologous_superfamily
IPR041899	MAGE_WH2	Homologous_superfamily

Pfam: PF01454, PF12440

UniProt features (10 total): compositionally biased region 4, region of interest 2, sequence variant 2, chain 1, domain 1

Structure

Experimental structures (PDB)

3 structures.

PDB	Method	Resolution (Å)
7QPJ	X-RAY DIFFRACTION	1.54
7PDW	X-RAY DIFFRACTION	1.82
7PBC	X-RAY DIFFRACTION	2.04

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-P43363-F1	74.28	0.51

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 43 (showing top): MODULE_59, BROWNE_HCMV_INFECTION_48HR_DN, KOYAMA_SEMA3B_TARGETS_UP, BROWNE_HCMV_INFECTION_14HR_DN, SHEN_SMARCA2_TARGETS_DN, GOMF_HISTONE_DEACETYLASE_BINDING, ZHOU_INFLAMMATORY_RESPONSE_LIVE_UP, chrXq28, GOBP_NEGATIVE_REGULATION_OF_TRANSCRIPTION_BY_RNA_POLYMERASE_II, PURBEY_TARGETS_OF_CTBP1_NOT_SATB1_DN, GOBP_NEGATIVE_REGULATION_OF_NUCLEOBASE_CONTAINING_COMPOUND_METABOLIC_PROCESS, FOSTER_KDM1A_TARGETS_UP, KRAS.KIDNEY_UP.V1_DN, MIR335_3P, MIR196A_1_3P

GO Biological Process (1): negative regulation of transcription by RNA polymerase II (GO:0000122)

GO Molecular Function (1): histone deacetylase binding (GO:0042826)

GO Cellular Component (3): nucleus (GO:0005634), nucleoplasm (GO:0005654), cytosol (GO:0005829)

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	2
regulation of transcription by RNA polymerase II	1
transcription by RNA polymerase II	1
negative regulation of DNA-templated transcription	1
enzyme binding	1
intracellular membrane-bounded organelle	1
nuclear lumen	1
cytoplasm	1

Protein interactions and networks

STRING

640 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
MAGEA10	CTAG1A	P78358	720
MAGEA10	PMEL	P40967	665
MAGEA10	CTAG2	O75638	663
MAGEA10	CSAG2	Q9Y5P2	657
MAGEA10	SSX4	O60224	594
MAGEA10	GAGE4	P0DSO3	574
MAGEA10	CSAG1	Q6PB30	486
MAGEA10	CT45A1	Q5HYN5	482
MAGEA10	CT55	Q8WUE5	480
MAGEA10	XAGE1B	Q9HD64	480
MAGEA10	PRAME	P78395	479
MAGEA10	SSX1	Q16384	479
MAGEA10	CT47A11	Q5JQC4	474
MAGEA10	CSF2	P04141	462
MAGEA10	A0A1W2PQG5	A0A1W2PQG5	447

IntAct

12 interactions, top by confidence:

A	B	Type	Score
MAGEA4	MAGEB16	psi-mi:“MI:0914”(association)	0.530
MAGEB10	GTPBP10	psi-mi:“MI:0914”(association)	0.530
MAGEA10	POTEF	psi-mi:“MI:0914”(association)	0.530
MAGEA4	MAGEA8	psi-mi:“MI:0914”(association)	0.530
MAGEA10	XPO1	psi-mi:“MI:0914”(association)	0.350
NEK4	E2F8	psi-mi:“MI:0914”(association)	0.350
P		psi-mi:“MI:0914”(association)	0.350
MAGEA10	KANSL1L	psi-mi:“MI:0914”(association)	0.350

BioGRID (113): KAT2A (Affinity Capture-MS), KAT2B (Affinity Capture-MS), NCOA2 (Affinity Capture-MS), CSRP2BP (Affinity Capture-MS), YEATS2 (Affinity Capture-MS), MAGEA9B (Affinity Capture-MS), POTEF (Affinity Capture-MS), ACTB (Affinity Capture-MS), DDX42 (Affinity Capture-MS), CUX1 (Affinity Capture-MS), ANKRD32 (Affinity Capture-MS), TRIM24 (Affinity Capture-MS), PHF8 (Affinity Capture-MS), SATB2 (Affinity Capture-MS), NRIP1 (Affinity Capture-MS)

ESM2 similar proteins: A0A0J9YX57, A1A5P9, A2A368, A2A9R3, A8MXT2, B2KFW1, O15479, O15480, O15481, O15553, P0C6Y7, P10073, P17040, P25233, P43355, P43356, P43357, P43358, P43360, P43362, P43363, P43364, P43366, Q13342, Q16666, Q4R998, Q5PPP4, Q5RD14, Q6AY37, Q6PCZ4, Q8BQR7, Q8IWY8, Q8IX06, Q8N660, Q8N7X4, Q8TD90, Q96DU7, Q96LZ2, Q96M61, Q99608

Diamond homologs: A0A0J9YX57, A1A5P9, A2A368, A2A9R3, A6NCF6, A6QLI5, A8MXT2, O15479, O15480, O15481, O60732, P25233, P43355, P43356, P43357, P43358, P43360, P43361, P43362, P43363, P43364, P43365, P43366, Q12816, Q4R998, Q5PPP4, Q5RFC2, Q6AY37, Q6ITT4, Q6PCZ4, Q8BQR7, Q8N7X4, Q8TD90, Q8TD91, Q96JG8, Q96LZ2, Q96M61, Q96MG7, Q99608, Q9BE18

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

1 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	1
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

639 predictions. Top by Δscore:

Variant	Effect	Δscore
X:152135681:CAGGC:C	acceptor_gain	1.0000
X:152135759:A:AC	donor_gain	1.0000
X:152135760:C:CC	donor_gain	1.0000
X:152135683:GGCC:G	acceptor_loss	0.9900
X:152135684:GCC:G	acceptor_loss	0.9900
X:152135686:C:CA	acceptor_loss	0.9900
X:152135686:C:CC	acceptor_gain	0.9900
X:152135687:T:G	acceptor_loss	0.9900
X:152135760:CAGGT:C	donor_gain	0.9900
X:152135760:CAG:C	donor_gain	0.9800
X:152137469:T:TA	donor_gain	0.9800
X:152135760:CA:C	donor_gain	0.9700
X:152135760:CAGG:C	donor_gain	0.9700
X:152136965:CCTCC:C	acceptor_gain	0.9700
X:152136966:CTCCC:C	acceptor_gain	0.9700
X:152136967:TCCCT:T	acceptor_gain	0.9700
X:152138471:TCA:T	donor_loss	0.9700
X:152138472:CA:C	donor_loss	0.9700
X:152138474:CCTT:C	donor_loss	0.9700
X:152138478:G:A	donor_gain	0.9700
X:152138481:T:TA	donor_gain	0.9600
X:152137470:C:A	donor_gain	0.9500
X:152137849:T:C	donor_gain	0.9500
X:152138114:C:A	donor_gain	0.9500
X:152135683:GGC:G	acceptor_gain	0.9400
X:152135835:CTGA:C	acceptor_loss	0.9400
X:152137823:C:CT	donor_gain	0.9400
X:152137885:T:TA	donor_gain	0.9400
X:152136329:CAT:C	donor_gain	0.9300
X:152136968:CC:C	acceptor_gain	0.9300

AlphaMissense

2437 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
X:152135060:A:C	F187L	0.978
X:152135060:A:T	F187L	0.978
X:152135062:A:G	F187L	0.978
X:152134925:G:C	S232R	0.975
X:152134925:G:T	S232R	0.975
X:152134927:T:G	S232R	0.975
X:152134734:A:G	F296S	0.970
X:152134679:A:C	F314L	0.959
X:152134679:A:T	F314L	0.959
X:152134681:A:G	F314L	0.959
X:152134798:A:G	W275R	0.958
X:152134798:A:T	W275R	0.958
X:152134726:C:G	G299R	0.952
X:152134733:A:C	F296L	0.950
X:152134733:A:T	F296L	0.950
X:152134735:A:G	F296L	0.950
X:152134729:A:G	W298R	0.948
X:152134729:A:T	W298R	0.948
X:152134643:G:C	F326L	0.944
X:152134643:G:T	F326L	0.944
X:152134645:A:G	F326L	0.944
X:152134770:C:G	R284P	0.939
X:152135058:C:A	G188V	0.936
X:152135177:C:A	K148N	0.931
X:152135177:C:G	K148N	0.931
X:152134779:A:G	L281P	0.930
X:152134956:G:T	P222H	0.925
X:152134880:C:A	W247C	0.923
X:152134880:C:G	W247C	0.923
X:152134725:C:T	G299D	0.921

dbSNP variants (sampled 300 via entrez): RS1000042912 (X:152133846 C>A,T), RS1000429471 (X:152139213 C>T), RS1000679200 (X:152136920 G>A), RS1001364862 (X:152137428 G>C,T), RS1001838664 (X:152137642 A>G), RS1002323034 (X:152138434 T>A), RS1002565872 (X:152137970 C>A,T), RS1003435212 (X:152134116 A>G), RS1003487948 (X:152134467 T>C), RS1003832195 (X:152140462 T>C), RS1004170917 (X:152138483 C>G,T), RS1004221872 (X:152138652 G>A), RS1005437411 (X:152135021 G>A,T), RS1005836998 (X:152135675 G>A,C), RS1006442000 (X:152132853 T>C)

Disease associations

OMIM: gene MIM:300343 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

14 total (human), top 14 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Decitabine	affects expression, increases expression	2
FR900359	increases phosphorylation	1
2,4,6-tribromophenol	decreases expression	1
bisphenol A	decreases expression	1
decabromobiphenyl ether	decreases expression	1
tetrabromobisphenol A	decreases expression	1
CGP 52608	increases reaction, affects binding	1
2,2’,4,4’-tetrabromodiphenyl ether	decreases expression	1
pentabrominated diphenyl ether 100	decreases expression	1
hexabrominated diphenyl ether 153	decreases expression	1
Gemcitabine	decreases expression	1
Benzo(a)pyrene	increases expression	1
Silicon Dioxide	decreases expression	1
Valproic Acid	increases methylation	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.