MARCHF10

Summary

MARCHF10 (membrane associated ring-CH-type finger 10, HGNC:26655) is a protein-coding gene on chromosome 17q23.2, encoding Probable E3 ubiquitin-protein ligase MARCHF10 (Q8NA82). E3 ubiquitin-protein ligase.

MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Morokuma et al., 2007 [PubMed 17604280]).

Source: NCBI Gene 162333 — RefSeq curated summary.

At a glance

• Clinical variants (ClinVar): 135 total
• MANE Select transcript: NM_152598

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:26655
Approved symbol	MARCHF10
Name	membrane associated ring-CH-type finger 10
Location	17q23.2
Locus type	gene with protein product
Status	Approved
Aliases	FLJ35757, MARCH-X
Ensembl gene	ENSG00000173838
Ensembl biotype	protein_coding
OMIM	613337
Entrez	162333

Gene structure

Transcript identifiers

Ensembl transcripts: 14 — 11 protein_coding, 3 protein_coding_CDS_not_defined

ENST00000311269, ENST00000456609, ENST00000544856, ENST00000578346, ENST00000579620, ENST00000580520, ENST00000582358, ENST00000582568, ENST00000583600, ENST00000583803, ENST00000858437, ENST00000858438, ENST00000948244, ENST00000948245

RefSeq mRNA: 4 — MANE Select: NM_152598 NM_001100875, NM_001288779, NM_001288780, NM_152598

CCDS: CCDS11635, CCDS74122, CCDS74123

Canonical transcript exons

ENST00000311269 — 11 exons

Exon	Start	End
ENSE00001181022	62722488	62722597
ENSE00001181026	62724938	62725104
ENSE00001181031	62735931	62737332
ENSE00001235128	62705539	62705581
ENSE00001235133	62711231	62711344
ENSE00001235159	62744376	62744528
ENSE00001235168	62759835	62760006
ENSE00002703894	62808077	62808314
ENSE00003556993	62788480	62788599
ENSE00003564918	62701314	62701758
ENSE00003594122	62801646	62801752

Expression profiles

Bgee: expression breadth ubiquitous, 153 present calls, max score 98.05.

FANTOM5 (CAGE): breadth tissue_specific, TPM avg 0.5136 / max 242.6837, expressed in 143 samples.

FANTOM5 promoters (6 alternative TSS)

Promoter ID	TPM avg	Samples expressed
167439	0.2141	32
167435	0.1285	62
167438	0.1040	36
167440	0.0386	5
167437	0.0175	4
167441	0.0110	6

Top tissues by expression

253 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
left testis	UBERON:0004533	98.05	gold quality
right testis	UBERON:0004534	98.03	gold quality
right uterine tube	UBERON:0001302	96.82	gold quality
testis	UBERON:0000473	94.46	gold quality
olfactory segment of nasal mucosa	UBERON:0005386	88.64	gold quality
sperm	CL:0000019	85.77	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	84.76	gold quality
adenohypophysis	UBERON:0002196	84.32	gold quality
male germ line stem cell (sensu Vertebrata) in testis	CL:0000089 ∩ UBERON:0000473	84.23	gold quality
pituitary gland	UBERON:0000007	83.37	gold quality
bronchial epithelial cell	CL:0002328	79.52	gold quality
buccal mucosa cell	CL:0002336	79.01	gold quality
bronchus	UBERON:0002185	77.99	gold quality
fallopian tube	UBERON:0003889	73.82	gold quality
nerve	UBERON:0001021	72.99	gold quality
tibial nerve	UBERON:0001323	72.99	gold quality
nasal cavity mucosa	UBERON:0001826	72.69	gold quality
oviduct epithelium	UBERON:0004804	71.80	gold quality
adult organism	UBERON:0007023	71.72	gold quality
right lung	UBERON:0002167	71.66	gold quality
nasal cavity epithelium	UBERON:0005384	70.29	gold quality
caudate nucleus	UBERON:0001873	70.12	gold quality
myocardium	UBERON:0002349	69.93	gold quality
nucleus accumbens	UBERON:0001882	68.04	gold quality
putamen	UBERON:0001874	67.91	gold quality
left uterine tube	UBERON:0001303	67.33	gold quality
pancreatic ductal cell	CL:0002079	66.86	silver quality
metanephros cortex	UBERON:0010533	66.81	gold quality
islet of Langerhans	UBERON:0000006	66.40	gold quality
left ventricle myocardium	UBERON:0006566	66.39	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	yes	8.59

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

22 targeting MARCHF10, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-223-3P	99.99	70.14	1140
HSA-MIR-30A-3P	99.87	69.74	2928
HSA-MIR-30D-3P	99.87	69.92	2917
HSA-MIR-30E-3P	99.87	69.68	2942
HSA-MIR-4492	99.87	68.25	3611
HSA-MIR-1321	99.84	65.30	1811
HSA-MIR-4739	99.84	65.25	1832
HSA-MIR-4756-5P	99.84	64.98	1809
HSA-MIR-378G	99.71	64.90	1106
HSA-MIR-29B-2-5P	99.67	68.98	1726
HSA-MIR-545-5P	99.66	70.18	2308
HSA-MIR-6132	99.60	65.83	1554
HSA-MIR-6836-5P	99.60	65.62	1538
HSA-MIR-4328	99.57	71.06	4094
HSA-MIR-1207-5P	99.49	69.11	2983
HSA-MIR-3191-3P	99.45	63.94	356
HSA-MIR-942-3P	98.81	69.04	876
HSA-MIR-513B-3P	98.76	68.12	1577
HSA-MIR-583	98.71	67.44	1791
HSA-MIR-4463	98.56	66.05	1071
HSA-MIR-6810-5P	98.29	66.21	975
HSA-MIR-6880-5P	98.08	65.59	1282

Cross-species orthologs

2 orthologs

Organism	Symbol	Gene ID
mus_musculus	Marchf10	ENSMUSG00000078627
rattus_norvegicus	Marchf10	ENSRNOG00000007084

Paralogs (1): MARCHF7 (ENSG00000136536)

Protein

Protein identifiers

Probable E3 ubiquitin-protein ligase MARCHF10 — Q8NA82 (reviewed: Q8NA82)

Alternative names: Membrane-associated RING finger protein 10, Membrane-associated RING-CH protein X, RING finger protein 190, RING-type E3 ubiquitin transferase MARCHF10

All UniProt accessions (7): Q8NA82, A0A140VKA1, G3V1Q5, J3KTK3, J3KTN9, J3QLJ2, J3QRH7

UniProt curated annotations — full annotation on UniProt →

Function. E3 ubiquitin-protein ligase. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Domain organisation. The RING-CH-type zinc finger domain is required for E3 ligase activity.

Pathway. Protein modification; protein ubiquitination.

RefSeq proteins (4): NP_001094345, NP_001275708, NP_001275709, NP_689811* (*=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR011016	Znf_RING-CH	Domain
IPR013083	Znf_RING/FYVE/PHD	Homologous_superfamily
IPR042583	MARCH10_RING_CH-C4HC3	Domain
IPR052297	RING-CH-type_E3_ubiq-ligase	Family

Pfam: PF12906

UniProt features (23 total): binding site 8, region of interest 4, compositionally biased region 4, sequence variant 3, sequence conflict 2, chain 1, zinc finger region 1

Structure

Experimental structures (PDB)

0 structures.

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q8NA82-F1	50.33	0.08

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (8): 659; 662; 677; 679; 687; 690; 711; 714

Function

Pathways and Gene Ontology

Reactome pathways

0 pathways

MSigDB gene sets: 68 (showing top): TCF4_Q5, TGACATY_UNKNOWN, GOBP_POST_TRANSLATIONAL_PROTEIN_MODIFICATION, PPARA_01, NIKOLSKY_BREAST_CANCER_17Q21_Q25_AMPLICON, IVANOVA_HEMATOPOIESIS_STEM_CELL_LONG_TERM, STAT5A_01, GOMF_ACYLTRANSFERASE_ACTIVITY, GOMF_AMINOACYLTRANSFERASE_ACTIVITY, GOMF_UBIQUITIN_LIKE_PROTEIN_LIGASE_ACTIVITY, HSF2_01, DODD_NASOPHARYNGEAL_CARCINOMA_DN, MEISSNER_NPC_HCP_WITH_H3K4ME2, MIKKELSEN_IPS_HCP_WITH_H3_UNMETHYLATED, HSF1_01

GO Biological Process (1): protein ubiquitination (GO:0016567)

GO Molecular Function (5): zinc ion binding (GO:0008270), ubiquitin protein ligase activity (GO:0061630), protein binding (GO:0005515), transferase activity (GO:0016740), metal ion binding (GO:0046872)

GO Cellular Component (0):

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
protein modification by small protein conjugation	1
transition metal ion binding	1
ubiquitin-protein transferase activity	1
ubiquitin-like protein ligase activity	1
binding	1
catalytic activity	1
cation binding	1

Protein interactions and networks

STRING

634 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
MARCHF10	MARCHF11	A6NNE9	742
MARCHF10	MARCHF9	Q86YJ5	643
MARCHF10	MARCHF3	Q86UD3	627
MARCHF10	MARCHF4	Q9P2E8	582
MARCHF10	EFCAB3	Q8N7B9	570
MARCHF10	TSSK4	Q6SA08	514
MARCHF10	QRICH1	Q2TAL8	510
MARCHF10	CFAP100	Q494V2	471
MARCHF10	TEKT4	Q8WW24	466
MARCHF10	ROPN1	Q9HAT0	464
MARCHF10	CFAP44	Q96MT7	462
MARCHF10	CABYR	O75952	462
MARCHF10	METTL2A	Q96IZ6	453
MARCHF10	ODF2	Q5BJF6	452
MARCHF10	SPATA6	Q9NWH7	447

IntAct

34 interactions, top by confidence:

A	B	Type	Score
UNC45A	MARCHF10	psi-mi:“MI:0915”(physical association)	0.780
MARCHF10	UNC45A	psi-mi:“MI:0915”(physical association)	0.780
MARCHF10	NTAQ1	psi-mi:“MI:0915”(physical association)	0.560
MARCHF10	ANLN	psi-mi:“MI:0915”(physical association)	0.560
NTAQ1	MARCHF10	psi-mi:“MI:0915”(physical association)	0.560
ANLN	MARCHF10	psi-mi:“MI:0915”(physical association)	0.560
GCD7	MARCHF10	psi-mi:“MI:0915”(physical association)	0.560
MARCHF10	GCD7	psi-mi:“MI:0915”(physical association)	0.560
MARCHF10		psi-mi:“MI:0915”(physical association)	0.560
MARCHF10	HTRA2	psi-mi:“MI:0915”(physical association)	0.560
MARCHF10	JPH3	psi-mi:“MI:0915”(physical association)	0.560
MARCHF10	SPRED1	psi-mi:“MI:0915”(physical association)	0.560
SRPK2	MARCHF10	psi-mi:“MI:0217”(phosphorylation reaction)	0.440
MARCHF10	Dlg4	psi-mi:“MI:0407”(direct interaction)	0.440
CTBP1	MARCHF10	psi-mi:“MI:0915”(physical association)	0.370

BioGRID (10): MARCH10 (Two-hybrid), MARCH10 (Two-hybrid), MARCH10 (Two-hybrid), MARCH10 (Two-hybrid), MARCH10 (Two-hybrid), MARCH10 (Affinity Capture-MS), MARCH10 (Two-hybrid), APP (Reconstituted Complex), MARCH10 (Biochemical Activity), MARCH10 (Two-hybrid)

ESM2 similar proteins: A0A1B0GTS1, A0A1B0GWH4, A1A4L6, A1YGI6, A6NDR6, B8QB46, F1MJR8, F1QDF8, O35892, O35893, P09015, P15036, P15037, P23497, P52729, P59598, Q32NH9, Q3KRF1, Q3UM89, Q4G112, Q4V7E1, Q5M7N6, Q5ND04, Q5XIV2, Q5ZHX5, Q66IG8, Q6P1R3, Q6PCX9, Q6PJQ5, Q708W2, Q76I76, Q76I79, Q76N89, Q7M6U3, Q8AXQ4, Q8BVK9, Q8IUE0, Q8IUE1, Q8IWB6, Q8IXJ9

Diamond homologs: A0JN69, F4JKK0, P32225, P40318, P90489, Q0IH10, Q0P496, Q0VD59, Q1LVZ2, Q28EX7, Q28IK8, Q32L65, Q3TZ87, Q5I0I2, Q5PQ35, Q5R9W2, Q5T0T0, Q5XH39, Q5XI50, Q5XIE5, Q5XIV2, Q68FA7, Q6NZQ8, Q80TE3, Q86UD3, Q86YJ5, Q8BRX9, Q8NA82, Q8TCQ1, Q93227, Q99M02, Q9DBD2, Q9DHV7, Q9H992, Q9P0N8, Q9P2E8, Q9WV66, A6NNE9, A6P320, O60103

SIGNOR signaling

1 interactions.

A	Effect	B	Mechanism
Ub:E2	“up-regulates activity”	MARCHF10	ubiquitination

Disease & clinical

Clinical variants and AI predictions

ClinVar

135 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	89
Likely benign	19
Benign	17

Top pathogenic / likely-pathogenic (0)

SpliceAI

2615 predictions. Top by Δscore:

Variant	Effect	Δscore
17:62711225:ACTT:A	donor_loss	1.0000
17:62711226:CTTA:C	donor_loss	1.0000
17:62711227:TTACC:T	donor_loss	1.0000
17:62711228:TACC:T	donor_loss	1.0000
17:62711229:A:C	donor_loss	1.0000
17:62711229:AC:A	donor_gain	1.0000
17:62711230:CC:C	donor_gain	1.0000
17:62711230:CCCT:C	donor_gain	1.0000
17:62788595:CAAGC:C	acceptor_gain	1.0000
17:62801641:ATTAC:A	donor_loss	1.0000
17:62801642:TTAC:T	donor_loss	1.0000
17:62801643:TA:T	donor_loss	1.0000
17:62801644:AC:A	donor_loss	1.0000
17:62801645:CCTGA:C	donor_loss	1.0000
17:62711224:GACTT:G	donor_loss	0.9900
17:62711229:A:AC	donor_gain	0.9900
17:62711230:C:CC	donor_gain	0.9900
17:62711240:TTCC:T	donor_gain	0.9900
17:62711341:GTGCC:G	acceptor_loss	0.9900
17:62711342:TGCC:T	acceptor_loss	0.9900
17:62711343:GCC:G	acceptor_loss	0.9900
17:62711345:C:A	acceptor_loss	0.9900
17:62711345:C:CC	acceptor_gain	0.9900
17:62722482:TCTTA:T	donor_loss	0.9900
17:62722483:CTTAC:C	donor_loss	0.9900
17:62722484:TTACC:T	donor_loss	0.9900
17:62722485:TACC:T	donor_loss	0.9900
17:62722486:ACCT:A	donor_loss	0.9900
17:62722487:CC:C	donor_loss	0.9900
17:62722599:T:C	acceptor_gain	0.9900

AlphaMissense

5344 scored. Top likely-pathogenic:

Variant	Protein change	am_pathogenicity
17:62724960:C:A	W694C	0.996
17:62724960:C:G	W694C	0.996
17:62724962:A:G	W694R	0.995
17:62724962:A:T	W694R	0.995
17:62724974:A:G	C690R	0.994
17:62724985:A:T	V686D	0.994
17:62722561:C:G	C714S	0.993
17:62722562:A:T	C714S	0.993
17:62724958:A:G	L695P	0.992
17:62724972:G:C	C690W	0.991
17:62724973:C:G	C690S	0.991
17:62724974:A:T	C690S	0.991
17:62725067:A:G	C659R	0.991
17:62725060:A:G	I661T	0.990
17:62725066:C:G	C659S	0.990
17:62725067:A:T	C659S	0.990
17:62722562:A:G	C714R	0.989
17:62725007:A:G	C679R	0.989
17:62724996:G:C	S682R	0.988
17:62724996:G:T	S682R	0.988
17:62724998:T:G	S682R	0.988
17:62724970:A:G	L691P	0.987
17:62725057:C:G	C662S	0.986
17:62725058:A:G	C662R	0.986
17:62725058:A:T	C662S	0.986
17:62725065:A:C	C659W	0.985
17:62722560:A:C	C714W	0.984
17:62722570:C:T	C711Y	0.984
17:62722571:A:G	C711R	0.984
17:62724981:A:C	H687Q	0.984

dbSNP variants (sampled 300 via entrez): RS1000026204 (17:62787256 A>C), RS1000085879 (17:62701049 T>C), RS1000088082 (17:62740769 G>C), RS1000091224 (17:62799645 C>T), RS1000152299 (17:62701234 T>C), RS1000159338 (17:62787032 A>C), RS1000182228 (17:62701508 G>T), RS1000226476 (17:62768881 T>C), RS1000251984 (17:62737620 T>C), RS1000335183 (17:62757060 A>C), RS1000336203 (17:62725842 C>T), RS1000364693 (17:62739941 G>T), RS1000373874 (17:62779917 C>A), RS1000397620 (17:62773135 G>A), RS1000424940 (17:62707002 G>A)

Disease associations

OMIM: gene MIM:613337 | disease phenotypes: MIM:618906

GenCC curated gene-disease

Mondo (1): intellectual developmental disorder with autistic features and language delay, with or without seizures (MONDO:0030051)

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

0 associations (top):

Drugs & pharmacology

Drug and pharmacology data

Is drug target: no

PharmGKB: 1 entry (VIP=true, CPIC=false)

CTD chemical–gene interactions

12 total (human), top 12 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
ethyl-p-hydroxybenzoate	decreases expression	1
sodium arsenite	increases expression	1
benzo(e)pyrene	increases methylation	1
abrine	increases expression	1
Resveratrol	affects cotreatment, decreases expression	1
Air Pollutants	increases abundance, increases expression	1
Benzo(a)pyrene	affects methylation, decreases methylation, increases methylation	1
Copper	affects cotreatment, decreases expression	1
Methapyrilene	increases methylation	1
Smoke	increases expression	1
Tobacco Smoke Pollution	decreases expression	1
Particulate Matter	increases abundance, increases expression	1

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.