METTL21A

Summary

METTL21A (methyltransferase 21A, HSPA lysine, HGNC:30476) is a protein-coding gene on chromosome 2q33.3, encoding Protein N-lysine methyltransferase METTL21A (Q8WXB1). Protein-lysine methyltransferase that selectively trimethylates residues in heat shock protein 70 (HSP70) family members.

Enables ATPase binding activity; Hsp70 protein binding activity; and protein-lysine N-methyltransferase activity. Involved in peptidyl-lysine methylation. Part of protein-containing complex.

Source: NCBI Gene 151194 — RefSeq curated summary.

At a glance

• GWAS associations: 7
• Clinical variants (ClinVar): 30 total
• Druggable target: yes
• MANE Select transcript: NM_001127395

Identifiers

Gene identifiers

Field	Value
HGNC ID	HGNC:30476
Approved symbol	METTL21A
Name	methyltransferase 21A, HSPA lysine
Location	2q33.3
Locus type	gene with protein product
Status	Approved
Aliases	LOC151194, HCA557b, HSPA-KMT
Ensembl gene	ENSG00000144401
Ensembl biotype	protein_coding
OMIM	615257
Entrez	151194

Gene structure

Transcript identifiers

Ensembl transcripts: 22 — 19 protein_coding, 3 protein_coding_CDS_not_defined

ENST00000272839, ENST00000406927, ENST00000411432, ENST00000425132, ENST00000426075, ENST00000432416, ENST00000442521, ENST00000448007, ENST00000448823, ENST00000458426, ENST00000461419, ENST00000471128, ENST00000477919, ENST00000861060, ENST00000861061, ENST00000861062, ENST00000861063, ENST00000861064, ENST00000921189, ENST00000921190, ENST00000921191, ENST00000951163

RefSeq mRNA: 25 — MANE Select: NM_001127395 NM_001127395, NM_001308021, NM_001330130, NM_001330131, NM_001330132, NM_001330133, NM_001330134, NM_001330135, NM_001330136, NM_001330137, NM_001388427, NM_001388439, NM_001393563, NM_001393564, NM_001393565, NM_001393566, NM_001393567, NM_001393569, NM_001393570, NM_001393571, NM_001393572, NM_001393573, NM_001393574, NM_001393575, NM_145280

CCDS: CCDS2376, CCDS77514, CCDS82562, CCDS82563

Canonical transcript exons

ENST00000411432 — 4 exons

Exon	Start	End
ENSE00001623898	207625062	207625394
ENSE00001804558	207612739	207613443
ENSE00003460231	207624229	207624404
ENSE00003571470	207621806	207621917

Expression profiles

Bgee: expression breadth ubiquitous, 241 present calls, max score 98.10.

FANTOM5 (CAGE): breadth ubiquitous, TPM avg 8.8683 / max 58.3984, expressed in 1756 samples.

FANTOM5 promoters (4 alternative TSS)

Promoter ID	TPM avg	Samples expressed
33408	3.9625	1648
33409	3.1973	1179
33407	0.8808	404
33410	0.8277	450

Top tissues by expression

253 total, by Bgee expression score (0-100, higher = more expressed):

Tissue	Anatomy ID	Expression score	Quality
oocyte	CL:0000023	98.10	gold quality
secondary oocyte	CL:0000655	97.47	gold quality
monocyte	CL:0000576	89.86	gold quality
leukocyte	CL:0000738	89.76	gold quality
C1 segment of cervical spinal cord	UBERON:0006469	88.02	gold quality
primordial germ cell in gonad	CL:0000670 ∩ UBERON:0000991	87.95	gold quality
mucosa of transverse colon	UBERON:0004991	87.28	gold quality
prefrontal cortex	UBERON:0000451	87.22	gold quality
spinal cord	UBERON:0002240	86.97	gold quality
right lobe of liver	UBERON:0001114	86.78	gold quality
anterior cingulate cortex	UBERON:0009835	86.24	gold quality
Brodmann (1909) area 9	UBERON:0013540	86.19	gold quality
granulocyte	CL:0000094	86.09	gold quality
adrenal tissue	UBERON:0018303	85.97	gold quality
putamen	UBERON:0001874	85.87	gold quality
bone marrow	UBERON:0002371	85.87	gold quality
caudate nucleus	UBERON:0001873	85.84	gold quality
biceps brachii	UBERON:0001507	85.81	gold quality
dorsolateral prefrontal cortex	UBERON:0009834	85.81	gold quality
hypothalamus	UBERON:0001898	85.56	gold quality
ventricular zone	UBERON:0003053	85.26	gold quality
calcaneal tendon	UBERON:0003701	85.26	gold quality
lymph node	UBERON:0000029	84.75	gold quality
skeletal muscle tissue of biceps brachii	UBERON:0004502	84.74	silver quality
right frontal lobe	UBERON:0002810	84.73	gold quality
bone marrow cell	CL:0002092	84.72	gold quality
amygdala	UBERON:0001876	84.67	gold quality
nucleus accumbens	UBERON:0001882	84.63	gold quality
neocortex	UBERON:0001950	84.51	gold quality
frontal cortex	UBERON:0001870	84.49	gold quality

Single-cell (SCXA)

Detected in 1 experiment(s), a significant marker in 1.

Experiment	Marker?	Max mean expression
E-ANND-3	no	0.00

Regulation

Is transcription factor: no

miRNA regulators (miRDB)

149 targeting METTL21A, top 30 by miRDB confidence (max_score; target_count = how many genes the miRNA targets in total — lower means more specific):

miRNA	Max score	Avg score	miRNA target_count
HSA-MIR-5011-5P	100.00	83.46	5820
HSA-MIR-6867-5P	100.00	82.21	3464
HSA-MIR-190A-3P	100.00	80.35	5520
HSA-MIR-6851-5P	100.00	65.63	1294
HSA-MIR-3689D	100.00	66.14	1181
HSA-MIR-4510	100.00	66.60	2050
HSA-MIR-6127	100.00	66.76	2188
HSA-MIR-6129	100.00	66.46	2080
HSA-MIR-6130	100.00	66.69	2012
HSA-MIR-6133	100.00	66.48	2064
HSA-MIR-4747-5P	100.00	67.90	2681
HSA-MIR-5196-5P	100.00	67.98	2761
HSA-MIR-33A-5P	99.99	68.62	1055
HSA-MIR-33B-5P	99.99	68.58	1062
HSA-MIR-6077	99.99	68.04	2299
HSA-MIR-548C-3P	99.99	74.01	7587
HSA-MIR-12136	99.98	72.81	5713
HSA-MIR-548N	99.98	71.94	4170
HSA-MIR-6778-3P	99.96	67.29	2693
HSA-MIR-2110	99.96	66.68	1930
HSA-MIR-1-3P	99.93	72.35	1914
HSA-MIR-206	99.93	72.50	1893
HSA-MIR-218-5P	99.93	72.22	2103
HSA-MIR-335-3P	99.93	73.36	4958
HSA-MIR-205-3P	99.92	69.92	3165
HSA-MIR-613	99.91	71.50	1710
HSA-MIR-374A-5P	99.90	71.34	2923
HSA-MIR-374B-5P	99.90	69.98	2734
HSA-MIR-9902	99.89	69.15	2250
HSA-MIR-95-5P	99.89	72.17	3973

Literature-anchored findings (GeneRIF, showing 2)

• METTL21A trimethylates Lys-561 in Hsp70 and corresponding residues in other isoforms (Hsc70, BiP) (PMID:23349634)
• methyltransferase METTL21A is the enzyme responsible for trimethylation of a conserved lysine residue found in several human Hsp70 (HSPA) proteins (PMID:23921388)

Cross-species orthologs

3 orthologs

Organism	Symbol	Gene ID
danio_rerio	mettl21a	ENSDARG00000012051
mus_musculus	Mettl21a	ENSMUSG00000025956
rattus_norvegicus	Mettl21a	ENSRNOG00000014212

Paralogs (5): VCPKMT (ENSG00000100483), EEF1AKMT3 (ENSG00000123427), METTL21C (ENSG00000139780), METTL23 (ENSG00000181038), METTL21EP (ENSG00000250878)

Protein

Protein identifiers

Protein N-lysine methyltransferase METTL21A — Q8WXB1 (reviewed: Q8WXB1)

Alternative names: HSPA lysine methyltransferase, HSPA-KMT, Hepatocellular carcinoma-associated antigen 557b, Methyltransferase-like protein 21A

All UniProt accessions (4): Q8WXB1, C9JES0, C9JSX1, H7BXH9

UniProt curated annotations — full annotation on UniProt →

Function. Protein-lysine methyltransferase that selectively trimethylates residues in heat shock protein 70 (HSP70) family members. Contributes to the in vivo trimethylation of Lys residues in HSPA1 and HSPA8. In vitro methylates ‘Lys-561’ in HSPA1, ‘Lys-564’ in HSPA2, ‘Lys-585’ in HSPA5, ‘Lys-563’ in HSPA6 and ‘Lys-561’ in HSPA8.

Subunit / interactions. Interacts with heat shock protein 70 family members; at least some of these proteins are methylation substrates.

Subcellular location. Cytoplasm.

Similarity. Belongs to the methyltransferase superfamily. METTL21 family.

Isoforms (2)

UniProt ID	Names	Canonical?
Q8WXB1-1	1	yes
Q8WXB1-2	2

RefSeq proteins (25): NP_001120867, NP_001294950, NP_001317059, NP_001317060, NP_001317061, NP_001317062, NP_001317063, NP_001317064, NP_001317065, NP_001317066, NP_001375356, NP_001375368, NP_001380492, NP_001380493, NP_001380494, NP_001380495, NP_001380496, NP_001380498, NP_001380499, NP_001380500, NP_001380501, NP_001380502, NP_001380503, NP_001380504, NP_660323 (=MANE)

Domains & families (InterPro)

ID	Name	Type
IPR019410	Methyltransf_16	Family
IPR029063	SAM-dependent_MTases_sf	Homologous_superfamily

Pfam: PF10294

Catalyzed reactions (Rhea), 1 shown:

• L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+) (RHEA:54192)

UniProt features (32 total): helix 10, strand 9, binding site 5, turn 2, splice variant 2, chain 1, sequence conflict 1, sequence variant 1, mutagenesis site 1

Structure

Experimental structures (PDB)

1 structures.

PDB	Method	Resolution (Å)
4LEC	X-RAY DIFFRACTION	2.28

Predicted structure (AlphaFold)

Model	pLDDT	Fraction very-high
AF-Q8WXB1-F1	91.57	0.85

Functional residue map

Curated UniProt residues grouped by drug-discovery relevance — catalytic, ligand-binding, modification, and mutation-validated positions. Source: UniProtKB sequence features.

Ligand- & substrate-binding residues (5): 47; 73–75; 94; 125; 143

Mutagenesis-validated functional residues (1):

Position	Phenotype
94	abolishes methyltransferase activity.

Function

Pathways and Gene Ontology

Reactome pathways

3 pathways

ID	Pathway
R-HSA-8876725	Protein methylation
R-HSA-392499	Metabolism of proteins
R-HSA-597592	Post-translational protein modification

MSigDB gene sets: 140 (showing top): GSE45365_CD8A_DC_VS_CD11B_DC_IFNAR_KO_DN, GSE45365_NK_CELL_VS_CD8A_DC_DN, WEI_MYCN_TARGETS_WITH_E_BOX, GOBP_PROTEIN_MATURATION, BILD_E2F3_ONCOGENIC_SIGNATURE, GOBP_PROTEIN_FOLDING, DODD_NASOPHARYNGEAL_CARCINOMA_UP, GOBP_METHYLATION, MARSON_BOUND_BY_FOXP3_UNSTIMULATED, LEE_RECENT_THYMIC_EMIGRANT, GOMF_HEAT_SHOCK_PROTEIN_BINDING, GOMF_N_METHYLTRANSFERASE_ACTIVITY, GOMF_PROTEIN_METHYLTRANSFERASE_ACTIVITY, GOMF_ATPASE_BINDING, GOMF_PROTEIN_FOLDING_CHAPERONE_BINDING

GO Biological Process (3): protein methylation (GO:0006479), negative regulation of protein folding (GO:1903333), methylation (GO:0032259)

GO Molecular Function (8): protein methyltransferase activity (GO:0008276), protein-lysine N-methyltransferase activity (GO:0016279), Hsp70 protein binding (GO:0030544), heat shock protein binding (GO:0031072), ATPase binding (GO:0051117), protein binding (GO:0005515), methyltransferase activity (GO:0008168), transferase activity (GO:0016740)

GO Cellular Component (4): nucleoplasm (GO:0005654), cytosol (GO:0005829), protein-containing complex (GO:0032991), cytoplasm (GO:0005737)

Reactome top-level categories

Rollup of top-2 pathways:

Category	Pathways
Post-translational protein modification	1
Metabolism of proteins	1

GO top-level categories

Rollup of top GO terms by namespace:

Category	Terms
cellular anatomical structure	3
protein alkylation	1
macromolecule methylation	1
protein folding	1
negative regulation of cellular process	1
regulation of protein folding	1
metabolic process	1
methyltransferase activity	1
catalytic activity, acting on a protein	1
protein methyltransferase activity	1
lysine N-methyltransferase activity	1
heat shock protein binding	1
protein-folding chaperone binding	1
protein binding	1
enzyme binding	1
binding	1
transferase activity, transferring one-carbon groups	1
catalytic activity	1
nuclear lumen	1
cytoplasm	1
cellular_component	1
intracellular anatomical structure	1

Protein interactions and networks

STRING

562 interactions, top by confidence (×1000):

Protein A	Protein B	Partner UniProt	Score
METTL21A	METTL22	Q9BUU2	797
METTL21A	METTL18	O95568	735
METTL21A	EEF1AKMT2	Q5JPI9	735
METTL21A	ETFBKMT	Q8IXQ9	716
METTL21A	KIN	O60870	687
METTL21A	EEF2KMT	Q96G04	674
METTL21A	EEF1AKMT1	Q8WVE0	666
METTL21A	CAMKMT	Q7Z624	649
METTL21A	CCNYL1	Q8N7R7	565
METTL21A	NTMT1	Q9BV86	537
METTL21A	METTL13	Q8N6R0	536
METTL21A	VCP	P55072	519
METTL21A	CSKMT	A8MUP2	501
METTL21A	EEF1AKMT4	P0DPD7	500
METTL21A	ANTKMT	Q9BQD7	489

IntAct

47 interactions, top by confidence:

A	B	Type	Score
METTL21A	FAM200C	psi-mi:“MI:0915”(physical association)	0.740
FAM200C	METTL21A	psi-mi:“MI:0915”(physical association)	0.740
LNX1	METTL21A	psi-mi:“MI:0915”(physical association)	0.670
METTL21A	LNX1	psi-mi:“MI:0915”(physical association)	0.670
ATRIP	METTL21A	psi-mi:“MI:0915”(physical association)	0.560
METTL21A	ATRIP	psi-mi:“MI:0915”(physical association)	0.560
ZNF655	METTL21A	psi-mi:“MI:0915”(physical association)	0.560
METTL21A	ZNF620	psi-mi:“MI:0915”(physical association)	0.560
METTL21A	MEI4	psi-mi:“MI:0915”(physical association)	0.560
METTL21A	ZNF655	psi-mi:“MI:0915”(physical association)	0.560
METTL21A	LZTS1	psi-mi:“MI:0915”(physical association)	0.560
MEOX2	METTL21A	psi-mi:“MI:0915”(physical association)	0.560
METTL21A	ZRANB1	psi-mi:“MI:0915”(physical association)	0.560
METTL21A	PFDN5	psi-mi:“MI:0915”(physical association)	0.560
MSRB3	METTL21A	psi-mi:“MI:0915”(physical association)	0.560
ZNF620	METTL21A	psi-mi:“MI:0915”(physical association)	0.560
MEI4	METTL21A	psi-mi:“MI:0915”(physical association)	0.560
ARPC4	METTL21A	psi-mi:“MI:0915”(physical association)	0.560
METTL21A	BAG3	psi-mi:“MI:0914”(association)	0.530
hspa1a_hspa1b_human-1	SHTN1	psi-mi:“MI:0914”(association)	0.350
METTL21A	BAG2	psi-mi:“MI:0914”(association)	0.350
METTL21A	HSPA4L	psi-mi:“MI:0914”(association)	0.350

BioGRID (56): METTL21A (Two-hybrid), METTL21A (Two-hybrid), HSPA8 (Affinity Capture-MS), HSPA4 (Affinity Capture-MS), ZBED8 (Two-hybrid), HSPA8 (Affinity Capture-MS), HSPBP1 (Affinity Capture-MS), BAG3 (Affinity Capture-MS), HSPA4 (Affinity Capture-MS), SSA1 (Biochemical Activity), SSA3 (Biochemical Activity), HSPA8 (Biochemical Activity), METTL21A (Two-hybrid), METTL21A (Two-hybrid), METTL21A (Two-hybrid)

ESM2 similar proteins: A2RU49, A3KCL7, A4FV42, A7MBI7, A7RDN6, D3ZDM7, D3ZLY0, O88587, O95671, P11172, P13439, P13676, P13798, P17256, P21964, P22734, P25409, P31228, P31754, P51175, P80227, Q03426, Q0V8R7, Q14CH1, Q29RU9, Q3TY86, Q4JIJ2, Q5E9T8, Q5H879, Q5R514, Q5U2W9, Q6GM82, Q86TI2, Q86WA6, Q8BGT5, Q8BVG4, Q8QZR5, Q8R164, Q8WXB1, Q922Z0

Diamond homologs: A2AA28, A4FV42, A4FV98, A4IGU3, D3YWP0, O14118, Q28IN4, Q5BLD8, Q5RE14, Q5RJL2, Q6DJF8, Q86XA0, Q8CDZ2, Q8WXB1, Q96AZ1, Q9CQL0, A6NDL7, A6QP81, A7IQW5, P0CU27, P53970, Q2KIJ2, Q58DC7, Q5VZV1, Q8BLU2, Q8C436, Q8R1C6, Q9BUU2, Q9H867, F4JNX3, O95568, P40389, P47163, Q4KM84, Q55DL2, Q9CZ09, Q7S634, P0CP44, P0CP45, P64840

SIGNOR signaling

0 interactions.

Disease & clinical

Clinical variants and AI predictions

ClinVar

30 variants total. Per-class counts are floors (≥ shown; pagination cap):

Classification	Count (floor)
Pathogenic	0
Likely pathogenic	0
Uncertain significance	25
Likely benign	0
Benign	0

Top pathogenic / likely-pathogenic (0)

SpliceAI

964 predictions. Top by Δscore:

Variant	Effect	Δscore
2:207625060:ACC:A	donor_gain	1.0000
2:207625061:CCC:C	donor_gain	1.0000
2:207625090:T:TA	donor_gain	1.0000
2:207613056:T:TA	donor_gain	0.9900
2:207624224:CTTA:C	donor_gain	0.9900
2:207624225:TTA:T	donor_loss	0.9900
2:207624226:TACCG:T	donor_loss	0.9900
2:207624227:A:AC	donor_gain	0.9900
2:207624227:A:C	donor_loss	0.9900
2:207624228:C:CA	donor_loss	0.9900
2:207624228:C:CC	donor_gain	0.9900
2:207624228:CCG:C	donor_gain	0.9900
2:207624294:T:C	acceptor_gain	0.9900
2:207624294:T:TC	acceptor_gain	0.9900
2:207625061:CCCCT:C	donor_gain	0.9900
2:207596912:A:AG	acceptor_gain	0.9800
2:207596912:AG:A	acceptor_gain	0.9800
2:207596913:G:GG	acceptor_gain	0.9800
2:207596913:GG:G	acceptor_gain	0.9800
2:207621804:AC:A	donor_gain	0.9800
2:207621805:CC:C	donor_gain	0.9800
2:207624403:ACCTG:A	acceptor_loss	0.9800
2:207624404:CCTGC:C	acceptor_loss	0.9800
2:207624405:CTGC:C	acceptor_loss	0.9800
2:207624406:T:A	acceptor_loss	0.9800
2:207625060:AC:A	donor_gain	0.9800
2:207625061:CC:C	donor_gain	0.9800
2:207625170:AGGAT:A	donor_gain	0.9800
2:207596908:TTGTA:T	acceptor_loss	0.9700
2:207596909:TGTA:T	acceptor_loss	0.9700

AlphaMissense

0 scored. Top likely-pathogenic:

dbSNP variants (sampled 300 via entrez): RS1000146686 (2:207598956 C>G,T), RS1000180340 (2:207621257 A>T), RS1000195748 (2:207620975 A>G), RS1000408351 (2:207614759 A>T), RS1000480716 (2:207619164 A>G), RS1000502749 (2:207586319 A>G), RS1000512868 (2:207619586 C>T), RS1000793142 (2:207612972 A>G), RS1000804015 (2:207591328 C>G,T), RS1000888066 (2:207584847 T>G), RS1000954407 (2:207625502 C>A,G,T), RS1001006886 (2:207625794 G>A), RS1001067516 (2:207592545 T>C), RS1001168967 (2:207591427 GTTTTGT>G), RS1001253481 (2:207584513 C>G,T)

Disease associations

OMIM: gene MIM:615257 | disease phenotypes:

GenCC curated gene-disease

Mondo (0):

Orphanet (0):

HPO phenotypes

0 total (0 of 0 shown, HPO-id order):

GWAS associations

7 associations (top):

Study	Trait	p-value
GCST001751_1	Opioid sensitivity	8.000000e-07
GCST002337_115	Amyotrophic lateral sclerosis (sporadic)	2.000000e-07
GCST004279_34	Systolic blood pressure	6.000000e-16
GCST004776_34	Systolic blood pressure	9.000000e-11
GCST004776_84	Systolic blood pressure	1.000000e-07
GCST007267_294	Systolic blood pressure	1.000000e-10
GCST010242_253	HDL cholesterol levels	1.000000e-09

EFO canonical traits (2, from GWAS)

EFO ID	Trait name
EFO:0006335	systolic blood pressure
EFO:0004612	high density lipoprotein cholesterol measurement

Drugs & pharmacology

Drug and pharmacology data

Is drug target: yes

ChEMBL targets (1): CHEMBL3588741 (SINGLE PROTEIN)

PharmGKB: 1 entry (VIP=true, CPIC=false)

PharmGKB clinical annotations

1 annotations.

Variant	Type	Level	Drugs	Phenotypes
rs7569963	Efficacy,Toxicity	3	antidepressants;citalopram	Depression

PharmGKB variants

1 variants.

Variant	Genes	Level	Score	#Clin annots	Drugs
rs7569963	CREB1, METTL21A	3	5.00	1	antidepressants;citalopram

CTD chemical–gene interactions

42 total (human), top 30 by PubMed support.

Chemical	Actions (top 5)	PubMed papers
Valproic Acid	affects cotreatment, decreases expression, affects expression	6
trichostatin A	affects cotreatment, decreases expression	2
Phenylmercuric Acetate	affects cotreatment, decreases expression	2
Tetrachlorodibenzodioxin	decreases expression	2
aristolochic acid I	decreases expression	1
bisphenol A	affects cotreatment, decreases expression	1
beta-lapachone	decreases expression	1
sodium arsenite	decreases expression	1
butyraldehyde	increases expression	1
hydroquinone	decreases expression	1
avobenzone	increases expression	1
entinostat	affects cotreatment, decreases expression	1
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide	affects cotreatment, decreases expression	1
erucylphospho-N,N,N-trimethylpropylammonium	increases expression	1
ICG 001	increases expression	1
dorsomorphin	affects cotreatment, decreases expression	1
2-(1H-indazol-4-yl)-6-(4-methanesulfonylpiperazin-1-ylmethyl)-4-morpholin-4-ylthieno(3,2-d)pyrimidine	decreases expression, increases response to substance	1
bisphenol S	affects cotreatment, decreases expression	1
4-(4-((5-(4,5-dimethyl-2-nitrophenyl)-2-furanyl)methylene)-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-1-yl)benzoic acid	increases expression	1
Bortezomib	increases expression	1
Temozolomide	decreases expression	1
Panobinostat	affects cotreatment, decreases expression	1
Acetaminophen	increases expression	1
Arsenic	affects methylation	1
Caffeine	decreases phosphorylation	1
Dexamethasone	affects cotreatment, decreases expression	1
Ethyl Methanesulfonate	decreases expression	1
Gasoline	affects cotreatment, increases abundance, increases expression	1
Hydrogen Peroxide	affects expression	1
Indomethacin	affects cotreatment, decreases expression	1

ChEMBL screening assays

8 unique, capped per target: 8 binding

Representative assays (with source publication via chembl_document):

Assay ID	Type	Description	Source paper
CHEMBL3592419	Binding	Inhibition of METTL21A (unknown origin) assessed as incorporation of tritium labeled methyl group from [3H]-SAM to substrate at 1 uM after 1 hr by scintillation proximity assay relative to control	Discovery of A-893, A New Cell-Active Benzoxazinone Inhibitor of Lysine Methyltransferase SMYD2. — ACS Med Chem Lett

Clinical trials (associated diseases)

0 trials via MONDO — disease-level, not drug-specific.